Mercurial > repos > iuc > deg_annotate
comparison deg_annotate.xml @ 1:e98d4ab5b5bc draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deg_annotate commit 44d3dae188cabf4a64dee7c1ebe41c855d95d1b0
author | iuc |
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date | Wed, 23 Jan 2019 07:47:22 -0500 |
parents | b42373cddb77 |
children |
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0:b42373cddb77 | 1:e98d4ab5b5bc |
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1 <tool id="deg_annotate" name="Annotate DESeq2/DEXSeq output tables" version="1.0"> | 1 <tool id="deg_annotate" name="Annotate DESeq2/DEXSeq output tables" version="1.1.0"> |
2 <description>Append useful information from annotation files to DESeq2/DEXSeq outputs</description> | 2 <description>Append annotation from GTF to differential expression tool outputs</description> |
3 <requirements> | 3 <requirements> |
4 <requirement type="package" version="2.27.0">bedtools</requirement> | 4 <requirement type="package" version="2.27.0">bedtools</requirement> |
5 <requirement type="package" version="0.6.4">bcbiogff</requirement> | 5 <requirement type="package" version="0.6.4">bcbiogff</requirement> |
6 </requirements> | 6 </requirements> |
7 <command> | 7 <command> |
19 <inputs> | 19 <inputs> |
20 <param name="input_table" | 20 <param name="input_table" |
21 type="data" | 21 type="data" |
22 format="tabular" | 22 format="tabular" |
23 argument="-in" | 23 argument="-in" |
24 label="Tabular output of DESeq2 or DEXSeq"/> | 24 label="Tabular output of DESeq2/edgeR/limma/DEXSeq"/> |
25 | 25 |
26 <param name="mode" type="select" argument="-m" label="Input file type"> | 26 <param name="mode" type="select" argument="-m" label="Input file type"> |
27 <option value="deseq2">DESeq2</option> | 27 <option value="degseq">DESeq2/edgeR/limma</option> |
28 <option value="dexseq">DEXseq</option> | 28 <option value="dexseq">DEXseq</option> |
29 </param> | 29 </param> |
30 | 30 |
31 <param name="annotation" | 31 <param name="annotation" |
32 type="data" | 32 type="data" |
90 <assert_contents> | 90 <assert_contents> |
91 <has_text_matching expression="FBgn0025111\+FBgn0003360:E005\tFBgn0025111\+FBgn0003360\tE005\t0.273966640920426\t6.62572321505791\t0.774068626605711\t0.378961325638675\tNA\t0.41523701984849\t1.17020080867011\t2.99101950917789\tchrX\t10780596\t10780661\t66\t-\t10\t0\t0\t0\t0\t0\t2\tFBtr0073425, FBtr0333963\tprotein_coding\+protein_coding\tAnt2\+sesB\tFBtr0073425:1,FBtr0333963:1"/> | 91 <has_text_matching expression="FBgn0025111\+FBgn0003360:E005\tFBgn0025111\+FBgn0003360\tE005\t0.273966640920426\t6.62572321505791\t0.774068626605711\t0.378961325638675\tNA\t0.41523701984849\t1.17020080867011\t2.99101950917789\tchrX\t10780596\t10780661\t66\t-\t10\t0\t0\t0\t0\t0\t2\tFBtr0073425, FBtr0333963\tprotein_coding\+protein_coding\tAnt2\+sesB\tFBtr0073425:1,FBtr0333963:1"/> |
92 </assert_contents> | 92 </assert_contents> |
93 </output> | 93 </output> |
94 </test> | 94 </test> |
95 <test expect_num_outputs="1"> | |
96 <param name="input_table" | |
97 value="edger_output.tabular"/> | |
98 <param name="annotation" | |
99 value="annotation.gtf"/> | |
100 <param name="mode" | |
101 value="degseq"/> | |
102 <output name="output"> | |
103 <assert_contents> | |
104 <has_text_matching expression="FBgn0039155\t-4.40480020002641\t5.8344799947229\t573.433304439283\t1.62187751744916e-36\t2.54342832286378e-32\tchr3R\t24141394\t24147490\t\+\tprotein_coding\tKal1"/> | |
105 </assert_contents> | |
106 </output> | |
107 </test> | |
95 </tests> | 108 </tests> |
96 | 109 |
97 <help> | 110 <help> |
98 <![CDATA[ | 111 <![CDATA[ |
99 | 112 |
100 **What it does** | 113 **What it does** |
101 | 114 |
102 This tool appends the output table of DESeq2 or DEXSeq with gene symbols, biotypes, positions etc. The information | 115 This tool appends the output table of DESeq2/edgeR/limma/DEXSeq with gene symbols, biotypes, positions etc. The information |
103 you want to add is configurable. This information should present in the input GTF/GFF file as attributes of feature | 116 you want to add is configurable. This information should present in the input GTF/GFF file as attributes of feature |
104 you choose. | 117 you choose. |
105 DEXSeq-Count tool is used to prepare the DEXSeq compatible annotation (flattened GTF file) from input GTF/GFF. In | 118 DEXSeq-Count tool is used to prepare the DEXSeq compatible annotation (flattened GTF file) from input GTF/GFF. In |
106 this process, the exons that appear multiple times, once for each transcript are collapsed to so called | 119 this process, the exons that appear multiple times, once for each transcript are collapsed to so called |
107 *exon counting bins*. Counting bins for parts of exons arise when an exonic region appears with different | 120 *exon counting bins*. Counting bins for parts of exons arise when an exonic region appears with different |