Mercurial > repos > iuc > deseq2

annotate get_deseq_dataset.R @ 25:de44f8eff84a draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/deseq2 commit 9eb6d07510ccf27d6499172d62c81661078ec57b"
author iuc
date Wed, 25 Nov 2020 18:36:55 +0000
parents 0696db066a5b
children 8fe98f7094de
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1 get_deseq_dataset <- function(sample_table, header, design_formula, tximport, txtype, tx2gene) {
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3 dir <- ""
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5 has_header <- !is.null(header)
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6 use_txi <- !is.null(tximport)
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7 if (use_txi) {
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8 if (is.null(tx2gene)) stop("A transcript-to-gene map or a GTF/GFF3 file is required for tximport")
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9 if (tolower(file_ext(tx2gene)) == "gff") {
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10 gff_file <- tx2gene
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11 } else {
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12 gff_file <- NULL
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13 tx2gene <- read.table(tx2gene, header = has_header)
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14 }
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15 }
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17 if (!use_txi & has_header) {
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18 countfiles <- lapply(as.character(sample_table$filename), read.delim, row.names = 1)
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19 tbl <- do.call("cbind", countfiles)
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20 colnames(tbl) <- rownames(sample_table) # take sample ids from header
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22 # check for htseq report lines (from DESeqDataSetFromHTSeqCount function)
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23 old_special_names <- c(
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24 "no_feature",
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25 "ambiguous",
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26 "too_low_aQual",
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27 "not_aligned",
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28 "alignment_not_unique"
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29 )
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30 special_rows <- (substr(rownames(tbl), 1, 1) == "_") | rownames(tbl) %in% old_special_names
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31 tbl <- tbl[!special_rows, , drop = FALSE]
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32
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33 dds <- DESeqDataSetFromMatrix(
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34 countData = tbl,
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35 colData = subset(sample_table, select = -filename),
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36 design = design_formula
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37 )
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38 } else if (!use_txi & !has_header) {
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40 # construct the object from HTSeq files
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41 dds <- DESeqDataSetFromHTSeqCount(
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42 sampleTable = sample_table,
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43 directory = dir,
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44 design = design_formula
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45 )
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46 colnames(dds) <- row.names(sample_table)
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48 } else {
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49 # construct the object using tximport
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50 library("tximport")
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51 txi_files <- as.character(sample_table$filename)
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52 labs <- row.names(sample_table)
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53 names(txi_files) <- labs
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54 if (!is.null(gff_file)) {
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55 # first need to make the tx2gene table
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56 # this takes ~2-3 minutes using Bioconductor functions
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57 suppressPackageStartupMessages({
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58 library("GenomicFeatures")
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59 })
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60 txdb <- makeTxDbFromGFF(gff_file)
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61 k <- keys(txdb, keytype = "TXNAME")
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62 tx2gene <- select(txdb, keys = k, columns = "GENEID", keytype = "TXNAME")
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63 # Remove 'transcript:' from transcript IDs (when gff_file is a GFF3 from Ensembl and the transcript does not have a Name)
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64 tx2gene$TXNAME <- sub("^transcript:", "", tx2gene$TXNAME) # nolint
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65 }
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66 try(txi <- tximport(txi_files, type = txtype, tx2gene = tx2gene))
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67 if (!exists("txi")) {
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68 # Remove version from transcript IDs in tx2gene...
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69 tx2gene$TXNAME <- sub("\\.[0-9]+$", "", tx2gene$TXNAME) # nolint
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70 # ...and in txi_files
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71 txi <- tximport(txi_files, type = txtype, tx2gene = tx2gene, ignoreTxVersion = TRUE)
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72 }
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73 dds <- DESeqDataSetFromTximport(
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74 txi,
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75 subset(sample_table, select = -c(filename)),
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76 design_formula
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77 )
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78 }
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79 return(dds)
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80 }