Mercurial > repos > iuc > dropletutils
diff scripts/generateTestData.Rscript @ 0:4cd9f0008d9c draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
author | iuc |
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date | Tue, 04 Jun 2019 17:19:52 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/scripts/generateTestData.Rscript Tue Jun 04 17:19:52 2019 -0400 @@ -0,0 +1,38 @@ +## +## conda install bioconductor-dropletutils bioconductor-dnabarcodes +## + +suppressWarnings(suppressPackageStartupMessages(library(DropletUtils))) +suppressWarnings(suppressPackageStartupMessages(library(DNABarcodes))) +library(Matrix) + +## Matrix with no column barcodes +set.seed(100) +##my.counts <- DropletUtils:::simCounts() +my.counts <- DropletUtils:::simCounts(large.rate=0.01, nlarge=500, nsmall=50, nempty=500) + +## Generate barcodes (size=10, edit=3) and set as headers +barcodes <- create.pool(10) +colnames(my.counts) <- barcodes[1:ncol(my.counts)] + +sce <- SingleCellExperiment(assays = list(counts = as.matrix(my.counts))) + +writeTSV <- function(fileout, obj){ + write.table(as.matrix(obj), file=fileout, col.names=NA, sep='\t', quote=FALSE) +} + +writeOut <- function(counts, fileout, typeout){ + if (typeout == "tsv"){ + writeTSV(fileout, counts) + } + else if (typeout == "h5ad"){ + write10xCounts(fileout, counts, type="HDF5", overwrite=TRUE) + } + else if (typeout == "directory"){ + write10xCounts(fileout, Matrix(counts), type="sparse", overwrite=TRUE) + } +} + +writeOut(Matrix(counts(sce)), "test-data", type="directory") +writeOut(counts(sce), "test-data.h5ad", type="h5ad") +writeOut(counts(sce), "test-data.tsv", type="tsv")