diff scripts/generateTestData.Rscript @ 0:4cd9f0008d9c draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
author iuc
date Tue, 04 Jun 2019 17:19:52 -0400
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/scripts/generateTestData.Rscript	Tue Jun 04 17:19:52 2019 -0400
@@ -0,0 +1,38 @@
+##
+## conda install bioconductor-dropletutils bioconductor-dnabarcodes
+##
+
+suppressWarnings(suppressPackageStartupMessages(library(DropletUtils)))
+suppressWarnings(suppressPackageStartupMessages(library(DNABarcodes)))
+library(Matrix)
+
+## Matrix with no column barcodes
+set.seed(100)
+##my.counts <- DropletUtils:::simCounts()
+my.counts <- DropletUtils:::simCounts(large.rate=0.01, nlarge=500, nsmall=50, nempty=500)
+
+## Generate barcodes (size=10, edit=3) and set as headers
+barcodes <- create.pool(10)
+colnames(my.counts) <- barcodes[1:ncol(my.counts)]
+
+sce <- SingleCellExperiment(assays = list(counts = as.matrix(my.counts)))
+
+writeTSV <- function(fileout, obj){
+    write.table(as.matrix(obj), file=fileout, col.names=NA, sep='\t', quote=FALSE)
+}
+
+writeOut <- function(counts, fileout, typeout){
+    if (typeout == "tsv"){
+        writeTSV(fileout, counts)
+    }
+    else if (typeout == "h5ad"){
+        write10xCounts(fileout, counts, type="HDF5", overwrite=TRUE)
+    }
+    else if (typeout == "directory"){
+        write10xCounts(fileout, Matrix(counts), type="sparse", overwrite=TRUE)
+    }
+}
+
+writeOut(Matrix(counts(sce)), "test-data", type="directory")
+writeOut(counts(sce), "test-data.h5ad", type="h5ad")
+writeOut(counts(sce), "test-data.tsv", type="tsv")