# HG changeset patch # User iuc # Date 1549714820 18000 # Node ID 555659de7321dde18c895042efcdce2745ed2ed3 # Parent fb9b9f0f2f06443cc51917acb5c8e7feb532d4e6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/edger commit 21f0bc6c64129122212a6d182fdf3e360617e629 diff -r fb9b9f0f2f06 -r 555659de7321 edger.R --- a/edger.R Tue Feb 05 20:57:30 2019 -0500 +++ b/edger.R Sat Feb 09 07:20:20 2019 -0500 @@ -526,7 +526,7 @@ flatCount[i] <- sumStatus["NotSig", ] # Write top expressions table - top <- topTags(res, n=Inf, sort.by="PValue") + top <- topTags(res, adjust.method=opt$pAdjOpt, n=Inf, sort.by="PValue") write.table(top, file=topOut[i], row.names=FALSE, sep="\t", quote=FALSE) linkName <- paste0("edgeR_", contrastData[i], ".tsv") diff -r fb9b9f0f2f06 -r 555659de7321 edger.xml --- a/edger.xml Tue Feb 05 20:57:30 2019 -0500 +++ b/edger.xml Sat Feb 09 07:20:20 2019 -0500 @@ -113,14 +113,14 @@ - + + help="Name of group that the counts files belong to (e.g. WT or Mut). NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each group must be a letter"> @@ -135,7 +135,7 @@ + help="You can choose to input the factor and group information for the samples from a file or manually enter below. NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each sample, factor and group must be a letter"> @@ -145,12 +145,12 @@ + help="Name of experiment factor of interest (e.g. Genotype). One factor must be entered and there must be two or more groups per factor. Additional factors (e.g. Batch) can be entered using the Insert Factor button below, see Help section below. NOTE: Please only use letters, numbers or underscores, and the first character of each factor must be a letter"> ^[\w]+$ + help="Enter the group names for the samples separated with commas e.g. WT,WT,WT,Mut,Mut,Mut. The order of the names must match the order of the samples in the columns of the count matrix. NOTE: Please only use letters, numbers or underscores (case sensitive), and the first character of each group must be a letter"> ^[\w,]+$ @@ -635,7 +635,7 @@ **Counts Data:** -The counts data can either be input as separate counts files (one sample per file) or a single count matrix (one sample per column). The rows correspond to genes, and columns correspond to the counts for the samples. Values must be tab separated, with the first row containing the sample/column labels and the first column containing the row/gene labels. Gene identifiers can be of any type but must be unique and not repeated within a counts file. +The counts data can either be input as separate counts files (one sample per file) or a single count matrix (one sample per column). The rows correspond to genes, and columns correspond to the counts for the samples. Values must be tab separated, with the first row containing the sample/column labels and the first column containing the row/gene labels. The sample labels must start with a letter. Gene identifiers can be of any type but must be unique and not repeated within a counts file. Example - **Separate Count Files**: @@ -698,10 +698,9 @@ Mut3 Mut b3 ========== ============ ========= -*Factor Name:* The name of the experimental factor being investigated e.g. Genotype, Treatment. One factor must be entered and spaces must not be used. Optionally, additional factors can be included, these are variables that might influence your experiment e.g. Batch, Gender, Subject. If additional factors are entered, an additive linear model will be used. +*Factor Name:* The name of the experimental factor being investigated e.g. Genotype, Treatment. One factor must be entered, the name should start with a letter and spaces must not be used. Optionally, additional factors can be included, these are variables that might influence your experiment e.g. Batch, Gender, Subject. If additional factors are entered, an additive linear model will be used. -*Groups:* The names of the groups for the factor. These must be entered in the same order as the samples (to which the groups correspond) are listed in the columns of the counts matrix. Spaces must not be used and if entered into the tool form above, the values should be separated by commas. - +*Groups:* The names of the groups for the factor. The names should start with a letter, and only contain letters, numbers and underscores, other characters such as spaces and hyphens must not be used. If entered into the tool form above, the order must be the same as the samples (to which the groups correspond) are listed in the columns of the counts matrix, with the values separated by commas. **Contrasts of Interest:** The contrasts you wish to make between levels.