Mercurial > repos > iuc > ena_upload
view ena_upload.xml @ 4:26ccb678abc8 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ena_upload commit ba358013c83e7dfffec895946d36585f237e54c5"
author | iuc |
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date | Tue, 19 Oct 2021 15:57:14 +0000 |
parents | 59bb6d34fca6 |
children | e1b3b37aa69f |
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<tool id="ena_upload" name="ENA Upload tool" version="@VERSION@" profile="20.01" license="MIT"> <macros> <token name="@VERSION@">0.4.1</token> <import>samples_macros.xml</import> </macros> <requirements> <requirement type="package" version="@VERSION@">ena-upload-cli</requirement> <requirement type="package" version="1.2.0">xlrd</requirement> </requirements> <stdio> <regex match="Oops" source="stderr" level="fatal"/> <regex match="different file names between command line and RUN table" source="stderr" level="fatal"/> </stdio> <command detect_errors="exit_code"><![CDATA[ mkdir ./submission_files; #set $studies_table_path = './submission_files/studies.tsv' #set $samples_table_path = './submission_files/samples.tsv' #set $experiments_table_path = './submission_files/experiments.tsv' #set $runs_table_path = './submission_files/runs.tsv' #set $studies_table_path_updated = './submission_files/studies_updated.tsv' #set $samples_table_path_updated = './submission_files/samples_updated.tsv' #set $experiments_table_path_updated = './submission_files/experiments_updated.tsv' #set $runs_table_path_updated = './submission_files/runs_updated.tsv' #set working_dir = os.getcwd() #if $action_options.input_format_conditional.input_format == "build_tables": python '$__tool_directory__/extract_tables.py' --action $action_options.action --out_dir ./submission_files --studies $studies_json; #end if #if $action_options.input_format_conditional.input_format == "excel_tables": python '$__tool_directory__/process_xlsx.py' #if $action_options.input_format_conditional.viral_submission == "true": --vir #end if #if $action_options.test_submit_parameters.submit_dev == "true": --dev #end if --action '$action_options.action' --form '$action_options.input_format_conditional.xlsx_file' --out_dir ./submission_files --verbose > '$output'; #end if #if $action_options.input_format_conditional.input_format != "user_generated_tables": cp $studies_table_path $studies_table_out && cp $samples_table_path $samples_table_out && cp $experiments_table_path $experiments_table_out && cp $runs_table_path $runs_table_out && #else: ln -s '$action_options.input_format_conditional.experiments_users_table' $experiments_table_path && ln -s '$action_options.input_format_conditional.studies_users_table' $studies_table_path && ln -s '$action_options.input_format_conditional.runs_users_table' $runs_table_path && ln -s '$action_options.input_format_conditional.samples_users_table' $samples_table_path && #end if #if $action_options.test_submit_parameters.dry_run == "false" and $action_options.test_submit == "False": webin_id=`grep 'username' $credentials`; if [ "\$webin_id" = "" ]; then ## No credentials in user defined preferences ## Fallback to global defined credentials (if exist) #import os #if os.path.isfile(os.environ.get('GALAXY_ENA_SECRETS', '')): credentials_path=\${GALAXY_ENA_SECRETS}; webin_id=`grep 'username' \$GALAXY_ENA_SECRETS`; if [ "\$webin_id" = "" ]; then echo "No global credentials defined. Check your GALAXY_ENA_SECRETS file or set your credentials via: User -> Preferences -> Manage Information"; exit 1; fi; #else: echo "No ENA credentials defined. Set your credentials via: User -> Preferences -> Manage Information"; exit 1; #end if else credentials_path='$credentials'; fi; #end if #if $action_options.test_submit == "True": credentials_path='test_fake_path'; #end if ## create the list of files to upload and make the symlinks #import re #set $files_to_upload = list() #if $action_options.input_format_conditional.input_format == "build_tables": #for $study in $action_options.input_format_conditional.conditional_viral_metadata.rep_study: #for $sample in $study.rep_sample: #for $experiment in $sample.rep_experiment: #for $run in $experiment.rep_runs: #for $file in $run.upload_files: #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) #if $action_options.input_format_conditional.add_extension == "true": #set $extension = '.fastq' #else #set $extension = '' #end if #if $file.is_of_type('fastq', 'fastqsanger'): ## compression output is defined as safename_reads_file so no need to symlink #set $safename_reads_file = $safename_reads_file + $extension + '.gz' gzip -c '$file' > $safename_reads_file && #else: #if $action_options.input_format_conditional.add_extension == "true": #if $file.is_of_type('fastq.gz', 'fastqsanger.gz'): #set $compression = '.gz' #elif $file.is_of_type('fastqsanger.bz2', 'fastq.bz2'): #set $compression = '.bz2' #end if #set $safename_reads_file = $safename_reads_file + $extension + $compression #end if ln -s '$file' $safename_reads_file && #end if $files_to_upload.append(str($safename_reads_file)) #end for #end for #end for #end for #end for #else: #if $action_options.input_format_conditional.run_input_format_conditional.run_input_format == 'paired_list': #for $pair in $action_options.input_format_conditional.run_input_format_conditional.paired_end_collection: #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $pair.name) ## Always need to add .fastq + compression suffix because the name is based on the pair name which has no extensions #if $pair.forward.is_of_type('fastq', 'fastqsanger'): ## compress the file, no need to create the link then ## always add the compression suffix (.gz) #set $safename_fwd_reads_file = $safename_reads_file + '_1' + 'fastq' + '.gz' gzip -c '$file' > $safename_fwd_reads_file && #else #if $pair.forward.is_of_type('fastq.gz', 'fastqsanger.gz'): #set $compression = '.gz' #elif $pair.forward.is_of_type('fastqsanger.bz2', 'fastq.bz2'): #set $compression = '.bz2' #end if #set $safename_fwd_reads_file = $safename_reads_file + '_1' + '.fastq' + $compression ln -s '$pair.forward' $safename_fwd_reads_file && #end if #if $pair.reverse.is_of_type('fastq', 'fastqsanger'): ## compress the file, no need to create the link then #set $safename_reverse_reads_file = $safename_reads_file + '_1' + '.fastq' + '.gz' gzip -c '$file' > $safename_rev_reads_file && #else #if $pair.reverse.is_of_type('fastqsanger.bz2', 'fastq.bz2'): #set $compression = '.bz2' #elif $pair.reverse.is_of_type('fastqsanger.gz', 'fastq.gz'): #set $compression = '.gz' #end if #set $safename_rev_reads_file = $safename_reads_file + '_2' + '.fastq' + $compression ln -s '$pair.reverse' $safename_rev_reads_file && #end if $files_to_upload.append(str($safename_fwd_reads_file)) $files_to_upload.append(str($safename_rev_reads_file)) #end for #end if #if $action_options.input_format_conditional.run_input_format_conditional.run_input_format == 'multiple_selection_list': #for $file in $action_options.input_format_conditional.run_input_format_conditional.data: #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) #if $file.is_of_type('fastq', 'fastqsanger'): ## always compress add the gz extension #if $action_options.input_format_conditional.run_input_format_conditional.add_extension == "true": #set $safename_reads_file = $safename_reads_file + 'fastq.gz' #else #set $safename_reads_file = $safename_reads_file + '.gz' #end if gzip -c '$file' > $safename_reads_file && #else #if $action_options.input_format_conditional.run_input_format_conditional.add_extension == "true": #if $file.is_of_type('fastq.gz', 'fastqsanger.gz'): #set $extension = 'fastq.gz' #elif $file.is_of_type('fastqsanger.bz2', 'fastq.bz2'): #set $extension = 'fastq.bz2' #end if #set $safename_reads_file = $safename_reads_file + $extension #end if ln -s '$file' $safename_reads_file && #end if $files_to_upload.append(str($safename_reads_file)) #end for #end if #end if #if $action_options.test_submit_parameters.dry_run == "false": #if $action_options.action == "add": ena-upload-cli --tool 'ena-upload-cli v@VERSION@ @ Galaxy' --action '$action_options.action' --center '$action_options.center' --secret \${credentials_path} --data #for $dataset in $files_to_upload: '$dataset' #end for --action add --experiment '$experiments_table_path' --study '$studies_table_path' --run '$runs_table_path' --sample '$samples_table_path' #if $action_options.input_format_conditional.input_format == "user_generated_tables": #if "$action_options.input_format_conditional.viral_submission" == "true": --checklist ERC000033 #end if #else: #if $action_options.input_format_conditional.input_format == "build_tables": #if $action_options.input_format_conditional.conditional_viral_metadata.viral_sample == "true": --checklist ERC000033 #end if #else: #if $action_options.input_format_conditional.viral_submission == "true": --checklist ERC000033 #end if #end if #end if #if $action_options.test_submit_parameters.submit_dev == "true": -d #end if >> '$output'; #end if #if $action_options.action == "modify": ena-upload-cli --tool 'ena-upload-cli v@VERSION@ @ Galaxy' --action '$action_options.action' --center '$action_options.center' --secret \${credentials_path} --data #for $dataset in $files_to_upload: '$dataset' #end for --action 'modify' --experiment '$experiments_table_path' --study '$studies_table_path' --run '$runs_table_path' --sample '$samples_table_path' #if $action_options.input_format_conditional.input_format == "user_generated_tables": #if "$action_options.input_format_conditional.viral_submission" == "true": --checklist ERC000033 #end if #else: #if $action_options.input_format_conditional.input_format == "build_tables": #if $action_options.input_format_conditional.conditional_viral_metadata.viral_sample == "true": --checklist ERC000033 #end if #else: #if $action_options.input_format_conditional.viral_submission == "true": --checklist ERC000033 #end if #end if #end if >> '$output'; #end if echo -e 'center_name\t$action_options.center' >> '$output'; echo -e 'action_option\t$action_options.action' >> '$output'; #if $action_options.input_format_conditional.input_format != "user_generated_tables": cp $studies_table_path_updated $studies_table_out 2>/dev/null; cp $samples_table_path_updated $samples_table_out 2>/dev/null; cp $experiments_table_path_updated $experiments_table_out 2>/dev/null; cp $runs_table_path_updated $runs_table_out 2>/dev/null; #end if #else: exit 0; #end if ]]></command> <configfiles> <configfile name="credentials"><![CDATA[ #set $webin_id = $__user__.extra_preferences.get('ena_webin_account|webin_id', "").strip() #set $password = $__user__.extra_preferences.get('ena_webin_account|password', "").strip() #if $webin_id != "": username: "$webin_id" password: "$password" #end if ]]></configfile> <configfile name="studies_json"> #import json #import re #if $action_options.input_format_conditional.input_format == "build_tables": #set $files_to_upload = list() #set $studies = list() #for $study in $action_options.input_format_conditional.conditional_viral_metadata.rep_study: #set samples = list() #for $sample in $study.rep_sample: #set experiments = list() #for $experiment in $sample.rep_experiment: #set runs = list() #for $run in $experiment.rep_runs: #set run_files = list() #for $file in $run.upload_files: #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) $run_files.append(str($safename_reads_file)) #end for $runs.append((str($run.run_base_name),$run_files)) #end for $experiments.append({'title':str($experiment.experiment_title),'experiment_design':str($experiment.experiment_design),'library_strategy':str($experiment.library_strategy),'library_source':str($experiment.library_source),'library_selection':str($experiment.library_selection),'library_layout':str($experiment.library_layout),'insert_size':str($experiment.insert_size),'library_construction_protocol':str($experiment.library_construction_protocol),'platform':str($experiment.platform),'instrument_model':str($experiment.instrument_model),'runs':$runs}) #end for #if $action_options.input_format_conditional.conditional_viral_metadata.viral_sample == "true": $samples.append({'title':str($sample.sample_title),'description':str($sample.sample_description),'tax_name':str($sample.scientific_name),'tax_id':str($sample.tax_id),'collection_date':str($sample.collection_date),'geo_location':str($sample.geo_location_country),'host_common_name':str($sample.host_common_name),'host_subject_id':str($sample.host_subject_id),'host_health_state':str($sample.host_health_state),'host_sex':str($sample.host_sex),'host_scientific_name':str($sample.host_scientific_name),'collector_name':str($sample.collector_name),'collecting_institution':str($sample.collecting_institution),'isolate':str($sample.isolate),'experiments':$experiments}) #else: $samples.append({'title':str($sample.sample_title),'description':str($sample.sample_description),'tax_name':str($sample.scientific_name),'tax_id':str($sample.tax_id),'experiments':$experiments}) #end if #end for $studies.append({'title':str($study.study_title),'type':str($study.study_type),'abstract':str($study.study_abstract),'pubmed_id':str($study.study_pubmed_id),'samples':$samples}) #end for #echo $json.dumps($studies) #end if </configfile> </configfiles> <inputs> <conditional name="action_options"> <param name="action" type="select" label="Action to execute"> <option value="add" selected="True">Add new data</option> <option value="modify">Modify metadata</option> </param> <when value="add"> <expand macro="test_submit_section"/> <param name="test_submit" type="hidden" value="False" /> <expand macro="table_inputs_macro" /> </when> <when value="modify"> <expand macro="test_submit_section"/> <param name="test_submit" type="hidden" value="False" /> <expand macro="table_inputs_macro" /> </when> </conditional> </inputs> <outputs> <data name="output" format="txt" label="ENA submission receipt"/> <data name="studies_table_out" format="tabular" label="Studies table"> <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> </data> <data name="samples_table_out" format="tabular" label="Samples table"> <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> </data> <data name="experiments_table_out" format="tabular" label="Experiments table"> <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> </data> <data name="runs_table_out" format="tabular" label="Runs table"> <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> </data> </outputs> <tests> <!--Test 1: excel input of VIRAL samples --> <test> <conditional name="action_options"> <param name="action" value="add"/> <section name="test_submit_parameters"> <param name="submit_dev" value="false" /> <param name="dry_run" value="true" /> </section> <conditional name="input_format_conditional"> <param name="input_format" value="excel_tables"/> <param name="viral_submission" value="True"/> <param name="xlsx_file" value="metadata_test_viral.xlsx"/> <conditional name="run_input_format_conditional"> <param name="add_extension" value="true"/> <param name="run_input_format" value="multiple_selection_list"/> <param name="data" value="sample.fq"/> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <output name="experiments_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="17"/> <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_alias\tsample_alias\tdesign_description\tlibrary_name\tlibrary_strategy\tlibrary_source\tlibrary_selection\tlibrary_layout\tinsert_size\tlibrary_construction_protocol\tplatform\tinstrument_model\tsubmission_date" /> <has_line_matching expression="e_(.*)_026\tmodify\taccession_ena\tNanopore sequencing\tSARS-CoV-2_genomes_01\ts_(.*)"/> </assert_contents> </output> <output name="studies_table_out"> <assert_contents> <has_n_lines n="2"/> <has_n_columns n="8"/> <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> <has_line_matching expression="SARS-CoV-2_genomes_01\tmodify\tENA_accession\tWhole-genome sequencing of SARS-CoV-2 from Covid-19 patients\tWhole Genome Sequencing\tWhole-genome sequences of SARS-CoV-2 from oro-pharyngeal swabs obtained from Covid-19 patients(.*)"/> </assert_contents> </output> <output name="samples_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="18"/> </assert_contents> </output> <output name="runs_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="8"/> <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> <has_line_matching expression="r_(.*)_026\tmodify\tena_run_accession\te_(.*)_026\tC026_exp5_clean.fastq.gz\tfastq\t\tsubmission_date_ENA"/> </assert_contents> </output> </test> <!--Test 2: excel input of VIRAL samples with extended columns--> <test> <conditional name="action_options"> <param name="action" value="add"/> <section name="test_submit_parameters"> <param name="submit_dev" value="false" /> <param name="dry_run" value="true" /> </section> <conditional name="input_format_conditional"> <param name="input_format" value="excel_tables"/> <param name="viral_submission" value="True"/> <param name="xlsx_file" value="metadata_test_viral_optional_columns.xlsx"/> <conditional name="run_input_format_conditional"> <param name="add_extension" value="true"/> <param name="run_input_format" value="multiple_selection_list"/> <param name="data" value="sample.fq"/> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <output name="experiments_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="17"/> <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_alias\tsample_alias\tdesign_description\tlibrary_name\tlibrary_strategy\tlibrary_source\tlibrary_selection\tlibrary_layout\tinsert_size\tlibrary_construction_protocol\tplatform\tinstrument_model\tsubmission_date" /> <has_line_matching expression="e_(.*)_026\tmodify\taccession_ena\tNanopore sequencing\tSARS-CoV-2_genomes_01\ts_(.*)"/> </assert_contents> </output> <output name="studies_table_out"> <assert_contents> <has_n_lines n="2"/> <has_n_columns n="8"/> <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> <has_line_matching expression="SARS-CoV-2_genomes_01\tmodify\tENA_accession\tWhole-genome sequencing of SARS-CoV-2 from Covid-19 patients\tWhole Genome Sequencing\tWhole-genome sequences of SARS-CoV-2 from oro-pharyngeal swabs obtained from Covid-19 patients(.*)"/> </assert_contents> </output> <output name="samples_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="42"/> </assert_contents> </output> <output name="runs_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="8"/> <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> <has_line_matching expression="r_(.*)_026\tmodify\tena_run_accession\te_(.*)_026\tC026_exp5_clean.fastq.gz\tfastq\t\tsubmission_date_ENA"/> </assert_contents> </output> </test> <!--Test 3: excel input of NON-VIRAL samples--> <test> <conditional name="action_options"> <param name="action" value="add"/> <section name="test_submit_parameters"> <param name="submit_dev" value="false" /> <param name="dry_run" value="true" /> </section> <conditional name="input_format_conditional"> <param name="input_format" value="excel_tables"/> <param name="viral_submission" value="False"/> <param name="xlsx_file" value="metadata_test_nonviral.xlsx"/> <conditional name="run_input_format_conditional"> <param name="add_extension" value="true"/> <param name="run_input_format" value="multiple_selection_list"/> <param name="data" value="sample.fq"/> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <output name="experiments_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="17"/> <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_alias\tsample_alias\tdesign_description\tlibrary_name\tlibrary_strategy\tlibrary_source\tlibrary_selection\tlibrary_layout\tinsert_size\tlibrary_construction_protocol\tplatform\tinstrument_model\tsubmission_date" /> </assert_contents> </output> <output name="studies_table_out"> <assert_contents> <has_n_lines n="2"/> <has_n_columns n="8"/> <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> </assert_contents> </output> <output name="samples_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="8"/> <has_line_matching expression="alias\ttitle\tscientific_name\tsample_description\tstatus\taccession\ttaxon_id\tsubmission_date"/> </assert_contents> </output> <output name="runs_table_out"> <assert_contents> <has_n_lines n="5"/> <has_n_columns n="8"/> <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> <has_line_matching expression="r_(.*)_026\tmodify\tena_run_accession\te_(.*)_026\tC026_exp5_clean.fastq.gz\tfastq\t\tsubmission_date_ENA"/> </assert_contents> </output> </test> <!--Test 4: failure on excel input of NON-VIRAL samples with runs PAIRED collection --> <test expect_failure="true"> <conditional name="action_options"> <param name="action" value="add"/> <section name="test_submit_parameters"> <param name="submit_dev" value="false" /> <param name="dry_run" value="false" /> </section> <conditional name="input_format_conditional"> <param name="add_extension" value="true"/> <param name="input_format" value="excel_tables"/> <param name="viral_submission" value="False"/> <param name="xlsx_file" value="metadata_test_nonviral.xlsx"/> <conditional name="run_input_format_conditional"> <param name="run_input_format" value="paired_list"/> <param name="paired_end_collection"> <collection type="list:paired"> <element name="paired_run_name"> <collection type="paired"> <element name="forward" value="1.fastqsanger.gz" ftype="fastqsanger.gz" /> <element name="reverse" value="2.fastqsanger.gz" ftype="fastqsanger.gz" /> </collection> </element> </collection> </param> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <assert_command> <has_text_matching expression="ena-upload-cli"/> <has_text_matching expression="--data 'paired_run_name_1.fastq.gz' 'paired_run_name_2.fastq.gz'"/> <has_text_matching expression="--action 'add' --center 'Some research center'"/> </assert_command> </test> <!--Test 5: build tables from user input fields NON-VIRAL samples--> <test> <conditional name="action_options"> <param name="action" value="add"/> <section name="test_submit_parameters"> <param name="submit_dev" value="false" /> <param name="dry_run" value="true" /> </section> <conditional name="input_format_conditional"> <param name="input_format" value="build_tables"/> <param name="add_extension" value="true"/> <conditional name="conditional_viral_metadata"> <param name="viral_sample" value="False"/> <repeat name="rep_study"> <param name="study_title" value="Test study title"/> <param name="study_abstract" value="Test study abstract"/> <param name="study_type" value="Epigenetics"/> <param name="study_pubmed_id" value="Test study pubmedID"/> <repeat name="rep_sample"> <param name="sample_title" value="Test Sample title"/> <param name="sample_description" value="Test Sample description"/> <param name="scientific_name" value="Test Sample scientific name"/> <param name="tax_id" value="Test Sample tax_id"/> <repeat name="rep_experiment"> <param name="experiment_title" value="Test experiment title"/> <param name="experiment_design" value="Test experiment design description"/> <param name="library_strategy" value="CTS"/> <param name="library_source" value="GENOMIC"/> <param name="library_selection" value="PCR"/> <param name="library_layout" value="SINGLE"/> <param name="insert_size" value="150"/> <param name="library_construction_protocol" value="Test library construction"/> <param name="platform" value="ILLUMINA"/> <param name="instrument_model" value="Illumina HiSeq 4000"/> <repeat name="rep_runs"> <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> </repeat> </repeat> </repeat> </repeat> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <output name="experiments_table_out"> <assert_contents> <has_n_lines n="2"/> <has_n_columns n="17"/> </assert_contents> </output> <output name="studies_table_out"> <assert_contents> <has_n_lines n="2"/> <has_n_columns n="8"/> <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> </assert_contents> </output> <output name="samples_table_out"> <assert_contents> <has_n_lines n="2"/> <has_n_columns n="8"/> </assert_contents> </output> <output name="runs_table_out"> <assert_contents> <has_n_lines n="3"/> <has_n_columns n="8"/> <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> </assert_contents> </output> </test> <!--Test 6: RUN failing build tables from user input fields NON-VIRAL samples--> <test expect_failure="true"> <conditional name="action_options"> <param name="action" value="add"/> <section name="test_submit_parameters"> <param name="submit_dev" value="true" /> <param name="dry_run" value="false" /> </section> <conditional name="input_format_conditional"> <param name="input_format" value="build_tables"/> <param name="add_extension" value="true"/> <conditional name="conditional_viral_metadata"> <param name="viral_sample" value="false"/> <repeat name="rep_study"> <param name="study_title" value="Test study title"/> <param name="study_abstract" value="Test study abstract"/> <param name="study_type" value="Epigenetics"/> <param name="study_pubmed_id" value="Test study pubmedID"/> <repeat name="rep_sample"> <param name="sample_title" value="Test Sample title"/> <param name="sample_description" value="Test Sample description"/> <param name="scientific_name" value="Test Sample scientific name"/> <param name="tax_id" value="Test Sample tax_id"/> <repeat name="rep_experiment"> <param name="experiment_title" value="Test experiment title"/> <param name="experiment_design" value="Test experiment design description"/> <param name="library_strategy" value="CTS"/> <param name="library_source" value="GENOMIC"/> <param name="library_selection" value="PCR"/> <param name="library_layout" value="SINGLE"/> <param name="insert_size" value="150"/> <param name="library_construction_protocol" value="Test library construction"/> <param name="platform" value="ILLUMINA"/> <param name="instrument_model" value="Illumina HiSeq 4000"/> <repeat name="rep_runs"> <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> </repeat> </repeat> </repeat> </repeat> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <assert_stdout> <has_text_matching expression="No ENA credentials defined"/> </assert_stdout> </test> <!--Test 7: with submit_test to skip credentials checksRUN failing build tables from user input fields NON-VIRAL samples also tests compression of uncompressed inputs and adding the .gz suffix --> <test expect_failure="true"> <conditional name="action_options"> <param name="action" value="add"/> <section name="test_submit_parameters"> <param name="submit_dev" value="true" /> <param name="dry_run" value="false" /> </section> <param name="test_submit" value="True"/> <conditional name="input_format_conditional"> <param name="add_extension" value="true"/> <param name="input_format" value="build_tables"/> <conditional name="conditional_viral_metadata"> <param name="viral_sample" value="false"/> <repeat name="rep_study"> <param name="study_title" value="Test study title"/> <param name="study_abstract" value="Test study abstract"/> <param name="study_type" value="Epigenetics"/> <param name="study_pubmed_id" value="Test study pubmedID"/> <repeat name="rep_sample"> <param name="sample_title" value="Test Sample title"/> <param name="sample_description" value="Test Sample description"/> <param name="scientific_name" value="Test Sample scientific name"/> <param name="tax_id" value="Test Sample tax_id"/> <repeat name="rep_experiment"> <param name="experiment_title" value="Test experiment title"/> <param name="experiment_design" value="Test experiment design description"/> <param name="library_strategy" value="CTS"/> <param name="library_source" value="GENOMIC"/> <param name="library_selection" value="PCR"/> <param name="library_layout" value="SINGLE"/> <param name="insert_size" value="150"/> <param name="library_construction_protocol" value="Test library construction"/> <param name="platform" value="ILLUMINA"/> <param name="instrument_model" value="Illumina HiSeq 4000"/> <repeat name="rep_runs"> <param name="run_base_name" value="run_from_hospital_X"/> <param name="upload_files" value="sample.fq,sample.fq" ftype="fastqsanger"/> </repeat> </repeat> </repeat> </repeat> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <assert_command> <has_text_matching expression="ena-upload-cli"/> <has_text_matching expression="--data 'sample.fq.fastq.gz' 'sample.fq.fastq.gz'"/> <has_text_matching expression="--action 'add' --center 'Some research center'"/> <not_has_text text="modify" /> </assert_command> <assert_stderr> <has_text_matching expression="Oops, the file test_fake_path does not exist"/> </assert_stderr> </test> <!--Test 8: viral submission - User input metadata - Add extension = False--> <test expect_failure="true"> <conditional name="action_options"> <param name="action" value="add"/> <section name="test_submit_parameters"> <param name="submit_dev" value="false" /> <param name="dry_run" value="false" /> </section> <param name="test_submit" value="True"/> <conditional name="input_format_conditional"> <param name="add_extension" value="False"/> <param name="input_format" value="build_tables"/> <conditional name="conditional_viral_metadata"> <param name="viral_sample" value="true"/> <repeat name="rep_study"> <param name="study_title" value="Test study title"/> <param name="study_abstract" value="Test study abstract"/> <param name="study_type" value="Epigenetics"/> <param name="study_pubmed_id" value="Test study pubmedID"/> <repeat name="rep_sample"> <param name="sample_title" value="Test Sample title"/> <param name="sample_description" value="Test Sample description"/> <param name="scientific_name" value="Test Sample scientific name"/> <param name="tax_id" value="Test Sample tax_id"/> <param name="collection_date" value="2020"/> <param name="geo_location_country" value="Belgium"/> <param name="host_common_name" value="Human"/> <param name="host_subject_id" value="Patient_001"/> <param name="host_health_state" value="healthy"/> <param name="host_sex" value="female"/> <param name="host_scientific_name" value="homo sapiens"/> <param name="collector_name" value="John The Collector"/> <param name="collecting_institution" value="Hospital 01"/> <param name="isolate" value="sample_001"/> <repeat name="rep_experiment"> <param name="experiment_title" value="Test experiment title"/> <param name="experiment_design" value="Test experiment design description"/> <param name="library_strategy" value="CTS"/> <param name="library_source" value="GENOMIC"/> <param name="library_selection" value="PCR"/> <param name="library_layout" value="SINGLE"/> <param name="insert_size" value="150"/> <param name="library_construction_protocol" value="Test library construction"/> <param name="platform" value="ILLUMINA"/> <param name="instrument_model" value="Illumina HiSeq 4000"/> <repeat name="rep_runs"> <param name="run_base_name" value="run_from_hospital_X"/> <param name="upload_files" value="1.fastqsanger.gz,2.fastqsanger.gz" ftype="fastqsanger.gz"/> </repeat> </repeat> </repeat> </repeat> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <assert_command> <has_text_matching expression="ena-upload-cli"/> <has_text_matching expression="--data '1.fastqsanger.gz' '2.fastqsanger.gz'"/> <has_text_matching expression="--action 'add' --center 'Some research center'"/> <has_text_matching expression="--checklist ERC000033"/> </assert_command> <assert_stderr> <has_text_matching expression="Oops, the file test_fake_path does not exist"/> </assert_stderr> </test> <!--Test 9: modify option and auto compression - viral submission - User input metadata--> <test expect_failure="true"> <conditional name="action_options"> <param name="action" value="modify"/> <section name="test_submit_parameters"> <param name="submit_dev" value="false" /> <param name="dry_run" value="false" /> </section> <param name="test_submit" value="True"/> <conditional name="input_format_conditional"> <param name="add_extension" value="False"/> <param name="input_format" value="build_tables"/> <conditional name="conditional_viral_metadata"> <param name="viral_sample" value="True"/> <repeat name="rep_study"> <param name="study_title" value="Test study title"/> <param name="study_abstract" value="Test study abstract"/> <param name="study_type" value="Epigenetics"/> <param name="study_pubmed_id" value="Test study pubmedID"/> <repeat name="rep_sample"> <param name="sample_title" value="Test Sample title"/> <param name="sample_description" value="Test Sample description"/> <param name="scientific_name" value="Test Sample scientific name"/> <param name="tax_id" value="Test Sample tax_id"/> <param name="collection_date" value="2020"/> <param name="geo_location_country" value="Belgium"/> <param name="host_common_name" value="Human"/> <param name="host_subject_id" value="Patient_001"/> <param name="host_health_state" value="healthy"/> <param name="host_sex" value="female"/> <param name="host_scientific_name" value="homo sapiens"/> <param name="collector_name" value="John The Collector"/> <param name="collecting_institution" value="Hospital 01"/> <param name="isolate" value="sample_001"/> <repeat name="rep_experiment"> <param name="experiment_title" value="Test experiment title"/> <param name="experiment_design" value="Test experiment design description"/> <param name="library_strategy" value="CTS"/> <param name="library_source" value="GENOMIC"/> <param name="library_selection" value="PCR"/> <param name="library_layout" value="SINGLE"/> <param name="insert_size" value="150"/> <param name="library_construction_protocol" value="Test library construction"/> <param name="platform" value="ILLUMINA"/> <param name="instrument_model" value="Illumina HiSeq 4000"/> <repeat name="rep_runs"> <param name="run_base_name" value="run_from_hospital_X"/> <param name="upload_files" value="sample.fq" ftype="fastqsanger"/> </repeat> </repeat> </repeat> </repeat> </conditional> </conditional> </conditional> <param name="center" value="Some research center"/> <assert_command> <has_text_matching expression="ena-upload-cli"/> <has_text_matching expression="--data 'sample.fq.gz'"/> <has_text_matching expression="--action 'modify' --center 'Some research center'"/> <has_text_matching expression="--checklist ERC000033"/> <not_has_text text="add" /> </assert_command> <assert_stderr> <has_text_matching expression="Oops, the file test_fake_path does not exist"/> </assert_stderr> </test> </tests> <help><![CDATA[ This is a wrapper for the ENA upload tool in https://github.com/usegalaxy-eu/ena-upload-cli The input metadata can be submitted following the tabular format of the templates in https://github.com/usegalaxy-eu/ena-upload-cli/tree/master/example_tables It is also possible to submit an excel file by following the template in https://drive.google.com/file/d/1ncC22--tW2v-EI-te_r86sAZujIPAjlX/view?usp=sharing For viral submissions a larger set of metadata is required, you can find the template in https://drive.google.com/file/d/1Gx78GKh58PmRjdmJ05DBbpObAL-3oUFX/view?usp=sharing ]]></help> <citations> </citations> </tool>