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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/ena_webin_cli commit 35b4a063e227ea4a2440e23c4df5c27e42c077cb
| author | iuc |
|---|---|
| date | Tue, 05 Aug 2025 11:53:57 +0000 |
| parents | |
| children | 93fac0ae859f |
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<tool id="ena_webin_cli" name="ENA Webin CLI" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" license="MIT" profile="24.2"> <description>Submission of consensus sequences to the European Nucleotide Archive (ENA)</description> <macros> <token name="@TOOL_VERSION@">8.1.1</token> <token name="@VERSION_SUFFIX@">0</token> </macros> <requirements> <requirement type="package" version="@TOOL_VERSION@">ena-webin-cli</requirement> <requirement type="package" version="1.76">biopython</requirement> <requirement type="package" version="5.3">pyyaml</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ #if $dry_run == "True": #set $credentials='./test_fake_path'; touch $credentials; echo "username:test_user" >> $credentials; echo "password:test_password" >> $credentials; #end if webin_id=`grep 'username' $credentials | cut -d':' -f2,2`; if [ "\$webin_id" = "" ]; then ## No credentials in user defined preferences ## Fallback to global defined credentials (if exist) #import os #if os.path.isfile(os.environ.get('GALAXY_ENA_SECRETS', '')): credentials_path=\${GALAXY_ENA_SECRETS}; webin_id=`grep 'username' \$GALAXY_ENA_SECRETS | cut -d':' -f2,2` | sed -e 's/^[[:space:]]*//'`; password=`grep 'password' \$GALAXY_ENA_SECRETS | cut -d':' -f2,2` | sed -e 's/^[[:space:]]*//'`; if [ "\$webin_id" = "" ]; then echo "No global credentials defined. Check your GALAXY_ENA_SECRETS file or set your credentials via: User -> Preferences -> Manage Information"; exit 1; fi; #else: echo "No ENA credentials defined. Set your credentials via: User -> Preferences -> Manage Information"; exit 1; #end if else password=`grep 'password' $credentials | cut -d':' -f2,2`; fi; ## create a manifest base file with the parameters that are identical for all sequences that will be submited (if submitting a multifasta) #set $manifest_base = 'manifest_base.tab' mkdir manifests; mkdir fasta; echo -e 'ASSEMBLY_TYPE\t$assembly_type' > $manifest_base; echo -e 'COVERAGE\t$coverage' >> $manifest_base; echo -e 'PROGRAM\t$assembly_program' >> $manifest_base; #if $min_gap_length: echo -e 'MINGAPLENGTH\t$min_gap_length' >> $manifest_base; #end if echo -e 'MOLECULETYPE\t$molecule_type' >> $manifest_base; #if $metadata_file_or_form.metadata_format == "file": #import re #for $file in $metadata_file_or_form.genome_fasta: #if $file.is_of_type('fasta'): #set $full_name = $file.element_identifier + '.gz' gzip -c '$file' > './fasta/$full_name'; #else: ln -s '$file' './fasta/$file.element_identifier'; #end if #end for ## process the input tables, this creates an intermediate file with information python3 '$__tool_directory__/process_input.py' $metadata_file_or_form.ena_receipt $genome_fasta_files './manifests' $manifest_base >> $webin_cli_log; center_name=`grep 'center_name' $metadata_file_or_form.ena_receipt | cut -f2,2 | tr -d '\n'`; #else: #set $generated_manifest='./manifests/generated_manifest.txt' cp $manifest_base $generated_manifest; #set $study_id = $metadata_file_or_form.study_accession #set $sample_id = $metadata_file_or_form.sample_accession echo -e 'STUDY\t$study_id' >> $generated_manifest; echo -e 'SAMPLE\t$sample_id' >> $generated_manifest; center_name='$metadata_file_or_form.center_name'; echo -e 'NAME\t$metadata_file_or_form.assembly_name' >> $generated_manifest; echo -e 'PLATFORM\t$metadata_file_or_form.sequencing_platform' >> $generated_manifest; #if $metadata_file_or_form.genome_fasta.is_of_type('fasta'): gzip -c $metadata_file_or_form.genome_fasta > consensus.fasta.gz; #else: ln -s $metadata_file_or_form.genome_fasta consensus.fasta.gz; #end if echo -e 'FASTA\tconsensus.fasta.gz' >> $generated_manifest; #end if #set $outputs_dir = 'outputs' mkdir $outputs_dir; #if $metadata_file_or_form.metadata_format == "file": ## iterate over the list of manifest - fasta generated by the process_input ## in case of errors, this list is empty while read line; do manifest=`echo \$line | cut -d' ' -f1,1`; echo "Submitting manifest \$manifest" >> $webin_cli_log; ena-webin-cli -context genome -userName "'\$webin_id'" -password "'\$password'" -centerName "'\$center_name'" -manifest \$manifest -inputDir './fasta' #if $submit_test == "true": -test #end if #if $dry_run == "true": -validate #else: -submit #end if -outputDir $outputs_dir >> $webin_cli_log; done < submit_list.tab; #else: ena-webin-cli #if $submit_test == "true": -test #end if #if $dry_run == "true": -validate #else: -submit #end if -context genome -manifest $generated_manifest -password "'\$password'" -userName "'\$webin_id'" -inputDir "./" -centerName "'\$center_name'" -outputDir $outputs_dir >> $webin_cli_log ; #end if tar -cf $webin_cli_outputs $outputs_dir ; ]]></command> <configfiles> <configfile name="credentials"><![CDATA[ #set $webin_id = $__user__.extra_preferences.get('ena_webin_account|webin_id', "").strip() #set $password = $__user__.extra_preferences.get('ena_webin_account|password', "").strip() #if $webin_id != "": username:$webin_id password:$password #end if ]]></configfile> <configfile name="genome_fasta_files"> #import json #import re #if $metadata_file_or_form.metadata_format == "file": #set $fasta_files_list = list() #for $file in $metadata_file_or_form.genome_fasta: $fasta_files_list.append(str($file.element_identifier)) #end for #echo json.dumps($fasta_files_list) #end if </configfile> </configfiles> <inputs> <param name="assembly_type" type="select" label="Assembly type"> <option value="clone">Clone</option> <option value="isolate">Isolate</option> <option value="COVID-19 outbreak">COVID-19 outbreak</option> </param> <param name="assembly_program" type="text" optional="False" label="Assembly program"/> <param name="molecule_type" type="select" label="Molecule type"> <option value="genomic RNA" selected="True">genomic RNA</option> <option value="viral cRNA">viral cRNA</option> <option value="genomic DNA">genomic DNA</option> </param> <param name="coverage" type="float" optional="False" value="10000" label="Coverage"/> <param name="min_gap_length" type="text" optional="True" label="Minimum gap length (optional)"/> <conditional name="metadata_file_or_form"> <param name="metadata_format" type="select" label="Select the method to load study and sample metadata"> <option value="form" selected="True">Fill in required submission metadata</option> <option value="file">I used the Galaxy ENA upload tool to submit the raw data: parse the received submission receipt</option> </param> <when value="file"> <param type="data" format="txt" name="ena_receipt" label="Submission receipt obtained from ENA upload tool"/> <param name="genome_fasta" type="data" label="Select the consensus sequence assembly files or a collection of them" format="fasta,fasta.gz" multiple="true"/> </when> <when value="form"> <param name="assembly_name" type="text" optional="False" label="Assembly name"/> <param name="study_accession" type="text" optional="False" label="Study accession"/> <param name="sample_accession" type="text" optional="False" label="Sample accession"/> <param name="sequencing_platform" type="text" optional="False" label="Sequencing platform"/> <param name="description" type="text" optional="True" value="" label="Description" help="Free text description of the genome assembly (optional)"/> <param name="center_name" type="text" optional="False" label="Center name"/> <param name="genome_fasta" type="data" label="Select the consensus sequence assembly file" format="fasta,fasta.gz"/> </when> </conditional> <param name="submit_test" type="boolean" truevalue="true" falsevalue="false" label="Submit to ENA test server" help="Uploads to the test server of ENA will not be made public and will be removed automatically in 24 hours. Performing a preliminary test upload is advised to check for errors with metadata structure. You can find these uploads at https://wwwdev.ebi.ac.uk/ena/." /> <param name="dry_run" type="boolean" truevalue="true" falsevalue="false" label="Validate files and metadata but do not submit" help="Generate input files and run Webin-CLI with -validate option. If 'No' is selected then it will validate and submit (-submit flag)"/> </inputs> <outputs> <collection name="generated_manifests" type="list" label="Generated manifests"> <discover_datasets pattern="(?P<designation>.+)\.txt" ext="txt" directory="manifests/"/> </collection> <data name="webin_cli_log" label="ENA submission log" format="txt"/> <data name="webin_cli_outputs" label="Webin cli outputs" format="tar"/> </outputs> <tests> <test> <param name="submit_test" value="true" /> <param name="dry_run" value="true" /> <param name="assembly_type" value="isolate"/> <param name="assembly_program" value="Test assembly program"/> <param name="molecule_type" value="viral cRNA"/> <param name="coverage" value="10000"/> <conditional name="metadata_file_or_form"> <param name="metadata_format" value="form"/> <param name="assembly_name" value="Test assembly name"/> <param name="study_accession" value="PRJEB49173"/> <param name="sample_accession" value="SAMEA11953908"/> <param name="sequencing_platform" value="Nanopore 0011"/> <param name="description" value="Test Description"/> <param name="center_name" value="Test center name"/> <param name="genome_fasta" value="phiX2.fasta"/> </conditional> <param name="min_gap_length" value="30"/> <output name="webin_cli_log"> <assert_contents> <has_n_lines n="4"/> <has_text_matching expression="ERROR: Invalid submission account user name or password."/> </assert_contents> </output> </test> <test> <param name="submit_test" value="true" /> <param name="dry_run" value="true" /> <param name="assembly_type" value="isolate"/> <param name="assembly_program" value="Test assembly program"/> <param name="molecule_type" value="viral cRNA"/> <param name="coverage" value="10000"/> <conditional name="metadata_file_or_form"> <param name="metadata_format" value="file"/> <param name="ena_receipt" value="receipt_sample_noPhiX.txt"/> <param name="genome_fasta" value="phiX2.fasta.gz,sample_alias_001.fasta.gz"/> </conditional> <param name="min_gap_length" value="30"/> <output name="webin_cli_log"> <assert_contents> <has_text_matching expression="Processing phiX2"/> <has_text_matching expression="No metadata found for sample phiX2"/> <has_text_matching expression="Processing sample_alias_001"/> <has_text_matching expression="Submitting manifest ./manifests/sample_alias_001.manifest.txt"/> <has_text_matching expression="ERROR: Invalid submission account user name or password. Please try enclosing your password in single quotes."/> </assert_contents> </output> </test> <test> <param name="submit_test" value="true" /> <param name="dry_run" value="true" /> <param name="assembly_type" value="isolate"/> <param name="assembly_program" value="Test assembly program"/> <param name="molecule_type" value="viral cRNA"/> <param name="coverage" value="10000"/> <conditional name="metadata_file_or_form"> <param name="metadata_format" value="file"/> <param name="ena_receipt" value="receipt_sample.txt"/> <param name="genome_fasta" value="sample_alias_001.fasta.gz"/> </conditional> <param name="min_gap_length" value="30"/> <output name="webin_cli_log"> <assert_contents> <has_text_matching expression="ERROR: Invalid submission account user name or password. Please try enclosing your password in single quotes."/> </assert_contents> </output> </test> </tests> <help><![CDATA[ This tool is a wrapper for the ENA Webin CLI submission tool (https://ena-docs.readthedocs.io/en/latest/submit/general-guide/webin-cli.html). .. class:: warningmark The ENA upload tool won't work unless you have provided an ENA Webin ID in User > Preferences > Manage Information > ENA Webin account details.]]></help> <citations> <citation type="doi">10.1093/nar/gkac1051</citation> </citations> </tool>
