Mercurial > repos > iuc > gatk2
annotate depth_of_coverage.xml @ 6:35c00763cb5c draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
author | iuc |
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date | Mon, 04 Jun 2018 05:38:15 -0400 |
parents | f244b8209eb8 |
children |
rev | line source |
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35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
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1 <tool id="gatk2_depth_of_coverage" name="Depth of Coverage" version="@VERSION@.1"> |
0 | 2 <description>on BAM files</description> |
3 <macros> | |
4 <import>gatk2_macros.xml</import> | |
5 </macros> | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
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changeset
|
6 <expand macro="requirements" /> |
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
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changeset
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7 <expand macro="version_command" /> |
0 | 8 <command interpreter="python">gatk2_wrapper.py |
9 ##--max_jvm_heap_fraction "1" | |
10 --stdout "${output_log}" | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
11 @BAM_INPUTS@ |
0 | 12 -p ' |
13 @JAR_PATH@ | |
14 -T "DepthOfCoverage" | |
15 \$GATK2_SITE_OPTIONS | |
16 | |
17 @THREADS@ | |
18 | |
19 #if $reference_source.reference_source_selector != "history": | |
20 -R "${reference_source.ref_file.fields.path}" | |
21 #end if | |
22 #if str( $input_calculate_coverage_over_genes ) != "None": | |
23 --calculateCoverageOverGenes "${input_calculate_coverage_over_genes}" | |
24 #end if | |
25 #if str( $partition_type ) != "None": | |
26 #for $pt in str( $partition_type ).split( ',' ): | |
27 --partitionType "${pt}" | |
28 #end for | |
29 #end if | |
30 --out "${output_per_locus_coverage}" | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
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31 |
0 | 32 #for $ct_group in $summary_coverage_threshold_group: |
33 --summaryCoverageThreshold "${ct_group.summary_coverage_threshold}" | |
34 #end for | |
35 --outputFormat "${output_format}" | |
36 ' | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
37 |
0 | 38 #include source=$standard_gatk_options# |
39 ##start analysis specific options | |
40 #if $analysis_param_type.analysis_param_type_selector == "advanced": | |
41 -p ' | |
42 ${analysis_param_type.ignore_deletion_sites} | |
43 ${analysis_param_type.include_deletions} | |
44 --maxBaseQuality "${analysis_param_type.max_base_quality}" | |
45 --maxMappingQuality "${analysis_param_type.max_mapping_quality}" | |
46 --minBaseQuality "${analysis_param_type.min_base_quality}" | |
47 --minMappingQuality "${analysis_param_type.min_mapping_quality}" | |
48 --nBins "${analysis_param_type.n_bins}" | |
49 ${analysis_param_type.omit_depth_output_at_each_base} | |
50 ${analysis_param_type.omit_interval_statistics} | |
51 ${analysis_param_type.omit_locus_table} | |
52 ${analysis_param_type.omit_per_sample_stats} | |
53 ${analysis_param_type.print_base_counts} | |
54 ${analysis_param_type.print_bin_endpoints_and_exit} | |
55 --start "${analysis_param_type.start}" | |
56 --stop "${analysis_param_type.stop}" | |
57 ' | |
58 #end if | |
59 ##Move additional files to final location | |
60 #if str( $partition_type ) != "None": | |
61 #set $partition_types = str( $partition_type ).split( ',' ) | |
62 #else: | |
63 #set $partition_types = [ 'sample' ] | |
64 #end if | |
65 #if 'sample' in $partition_types and ( str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.print_bin_endpoints_and_exit ) == "" ): | |
66 #if str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_per_sample_stats ) == "": | |
67 && mv ${output_per_locus_coverage}.sample_summary ${output_summary_sample} | |
68 && mv ${output_per_locus_coverage}.sample_statistics ${output_statistics_sample} | |
69 #end if | |
70 #if $gatk_param_type.gatk_param_type_selector == "advanced" and len( $gatk_param_type.input_interval_repeat ) and ( str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_interval_statistics ) == "" ): | |
71 && mv ${output_per_locus_coverage}.sample_interval_summary ${output_interval_summary_sample} | |
72 && mv ${output_per_locus_coverage}.sample_interval_statistics ${output_interval_statistics_sample} | |
73 #end if | |
74 #if str( $input_calculate_coverage_over_genes ) != "None": | |
75 && mv ${output_per_locus_coverage}.sample_gene_summary ${output_gene_summary_sample} | |
76 && mv ${output_per_locus_coverage}.sample_gene_statistics ${output_gene_statistics_sample} | |
77 #end if | |
78 #if str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_depth_output_at_each_base ) == "": | |
79 && mv ${output_per_locus_coverage}.sample_cumulative_coverage_counts ${output_cumulative_coverage_counts_sample} | |
80 && mv ${output_per_locus_coverage}.sample_cumulative_coverage_proportions ${output_cumulative_coverage_proportions_sample} | |
81 #end if | |
82 #end if | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
83 |
0 | 84 #if 'readgroup' in $partition_types and ( str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.print_bin_endpoints_and_exit ) == "" ): |
85 #if str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_per_sample_stats ) == "": | |
86 && mv ${output_per_locus_coverage}.read_group_summary ${output_summary_readgroup} | |
87 && mv ${output_per_locus_coverage}.read_group_statistics ${output_statistics_readgroup} | |
88 #end if | |
89 #if $gatk_param_type.gatk_param_type_selector == "advanced" and len( $gatk_param_type.input_interval_repeat ) and ( str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_interval_statistics ) == "" ): | |
90 && mv ${output_per_locus_coverage}.read_group_interval_summary ${output_interval_summary_readgroup} | |
91 && mv ${output_per_locus_coverage}.read_group_interval_statistics ${output_interval_statistics_readgroup} | |
92 #end if | |
93 #if str( $input_calculate_coverage_over_genes ) != "None": | |
94 && mv ${output_per_locus_coverage}.read_group_gene_summary ${output_gene_summary_readgroup} | |
95 && mv ${output_per_locus_coverage}.read_group_gene_statistics ${output_gene_statistics_readgroup} | |
96 #end if | |
97 #if str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_depth_output_at_each_base ) == "": | |
98 && mv ${output_per_locus_coverage}.read_group_cumulative_coverage_counts ${output_cumulative_coverage_counts_readgroup} | |
99 && mv ${output_per_locus_coverage}.read_group_cumulative_coverage_proportions ${output_cumulative_coverage_proportions_readgroup} | |
100 #end if | |
101 #end if | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
102 |
0 | 103 #if 'library' in $partition_types and ( str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.print_bin_endpoints_and_exit ) == "" ): |
104 #if str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_per_sample_stats ) == "": | |
105 && mv ${output_per_locus_coverage}.library_summary ${output_summary_library} | |
106 && mv ${output_per_locus_coverage}.library_statistics ${output_statistics_library} | |
107 #end if | |
108 #if $gatk_param_type.gatk_param_type_selector == "advanced" and len( $gatk_param_type.input_interval_repeat ) and ( str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_interval_statistics ) == "" ): | |
109 && mv ${output_per_locus_coverage}.library_interval_summary ${output_interval_summary_library} | |
110 && mv ${output_per_locus_coverage}.library_interval_statistics ${output_interval_statistics_library} | |
111 #end if | |
112 #if str( $input_calculate_coverage_over_genes ) != "None": | |
113 && mv ${output_per_locus_coverage}.library_gene_summary ${output_gene_summary_library} | |
114 && mv ${output_per_locus_coverage}.library_gene_statistics ${output_gene_statistics_library} | |
115 #end if | |
116 #if str( $analysis_param_type.analysis_param_type_selector ) == "basic" or str( $analysis_param_type.omit_depth_output_at_each_base ) == "": | |
117 && mv ${output_per_locus_coverage}.library_cumulative_coverage_counts ${output_cumulative_coverage_counts_library} | |
118 && mv ${output_per_locus_coverage}.library_cumulative_coverage_proportions ${output_cumulative_coverage_proportions_library} | |
119 #end if | |
120 #end if | |
121 | |
122 </command> | |
123 <inputs> | |
124 <conditional name="reference_source"> | |
125 <expand macro="reference_source_selector_param" /> | |
126 <when value="cached"> | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
127 <expand macro="input_bams_cached" /> |
0 | 128 <param name="ref_file" type="select" label="Using reference genome" help="-R,--reference_sequence &lt;reference_sequence&gt;"> |
129 <options from_data_table="gatk2_picard_indexes"> | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
130 <!-- <filter type="data_meta" key="dbkey" ref="input_bam" column="dbkey"/> does not yet work in a repeat...--> |
0 | 131 </options> |
132 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> | |
133 </param> | |
134 </when> | |
135 <when value="history"> <!-- FIX ME!!!! --> | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
136 |
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
137 <expand macro="input_bams_history" /> |
0 | 138 <param name="ref_file" type="data" format="fasta" label="Using reference file" help="-R,--reference_sequence &lt;reference_sequence&gt;" /> |
139 </when> | |
140 </conditional> | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
141 |
0 | 142 <param name="input_calculate_coverage_over_genes" type="data" format="data" label="RefSeq Rod" optional="True" help="-geneList,--calculateCoverageOverGenes &lt;calculateCoverageOverGenes&gt;" /> |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
143 |
0 | 144 <param name="partition_type" type="select" label="Partition type for depth of coverage" multiple="True" display="checkboxes" help="-pt,--partitionType &lt;partitionType&gt;"> |
145 <option value="sample" selected="True">sample</option> | |
146 <option value="readgroup">readgroup</option> | |
147 <option value="library">library</option> | |
148 </param> | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
149 |
0 | 150 <repeat name="summary_coverage_threshold_group" title="Summary coverage threshold" help="-ct,--summaryCoverageThreshold &lt;summaryCoverageThreshold&gt;"> |
151 <param name="summary_coverage_threshold" type="integer" value="15" label="for summary file outputs, report the % of bases covered to >= this number" /> | |
152 </repeat> | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
153 |
0 | 154 <param name="output_format" type="select" label="Output format" help="--outputFormat &lt;outputFormat&gt;" > |
155 <option value="csv">csv</option> | |
156 <option value="table">table</option> | |
157 <option value="rtable" selected="True">rtable</option> | |
158 </param> | |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
159 |
0 | 160 <expand macro="gatk_param_type_conditional" /> |
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
161 |
0 | 162 <expand macro="analysis_type_conditional"> |
163 <param name="ignore_deletion_sites" type="boolean" truevalue="--ignoreDeletionSites" falsevalue="" checked="False" label="Ignore sites consisting only of deletions" help="--ignoreDeletionSites" /> | |
164 <param name="include_deletions" type="boolean" truevalue="--includeDeletions" falsevalue="" checked="False" label="Include information on deletions" help="-dels,--includeDeletions" /> | |
165 <param name="max_base_quality" type="integer" value="127" label="Maximum quality of bases to count towards depth" help="--maxBaseQuality &lt;maxBaseQuality&gt;" /> | |
166 <param name="min_base_quality" type="integer" value="-1" label="Minimum quality of bases to count towards depth" help="-mbq,--minBaseQuality &lt;minBaseQuality&gt;" /> | |
167 <param name="max_mapping_quality" type="integer" value="2147483647" label="Maximum mapping quality of reads to count towards depth." help="--maxMappingQuality &lt;maxMappingQuality&gt;" /> | |
168 <param name="min_mapping_quality" type="integer" value="127" label="Minimum mapping quality of reads to count towards depth" help="-mmq,--minMappingQuality &lt;minMappingQuality&gt;" /> | |
169 <param name="n_bins" type="integer" value="499" label="Number of bins to use for granular binning" help="--nBins &lt;nBins&gt;" /> | |
170 <param name="omit_depth_output_at_each_base" type="boolean" truevalue="--omitDepthOutputAtEachBase" falsevalue="" checked="False" label="Omit the output of the depth of coverage at each base" help="-omitBaseOutput,--omitDepthOutputAtEachBase" /> | |
171 <param name="omit_interval_statistics" type="boolean" truevalue="--omitIntervalStatistics" falsevalue="" checked="False" label="Omit the per-interval statistics section" help="-omitIntervals,--omitIntervalStatistics" /> | |
172 <param name="omit_locus_table" type="boolean" truevalue="--omitLocusTable" falsevalue="" checked="False" label="Do not calculate the per-sample per-depth counts of loci" help="-omitLocusTable,--omitLocusTable" /> | |
173 <param name="omit_per_sample_stats" type="boolean" truevalue="--omitPerSampleStats" falsevalue="" checked="False" label="Omit the summary files per-sample." help="-omitSampleSummary,--omitPerSampleStats" /> | |
174 <param name="print_base_counts" type="boolean" truevalue="--printBaseCounts" falsevalue="" checked="False" label="Add base counts to per-locus output" help="-baseCounts,--printBaseCounts" /> | |
175 <param name="print_bin_endpoints_and_exit" type="boolean" truevalue="--printBinEndpointsAndExit" falsevalue="" checked="False" label="Print the bin values and exits immediately" help="--printBinEndpointsAndExit" /> | |
176 <param name="start" type="integer" value="1" label="Starting (left endpoint) for granular binning" help="--start &lt;start&gt;" /> | |
177 <param name="stop" type="integer" value="500" label="Ending (right endpoint) for granular binning" help="--stop &lt;stop&gt;" /> | |
178 </expand> | |
179 </inputs> | |
180 <outputs> | |
181 <data format="tabular" name="output_per_locus_coverage" label="${tool.name} on ${on_string} (per locus coverage)" > | |
182 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
183 <actions> | |
184 <conditional name="output_format"> | |
185 <when value="rtable"> | |
186 <action type="format"> | |
187 <option type="from_param" name="output_format" /> | |
188 </action> | |
189 </when> | |
190 <when value="csv"> | |
191 <action type="format"> | |
192 <option type="from_param" name="output_format" /> | |
193 </action> | |
194 </when> | |
195 </conditional> | |
196 </actions> | |
197 </data> | |
198 <data format="tabular" name="output_summary_sample" label="${tool.name} on ${on_string} (output summary sample)" > | |
199 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_per_sample_stats'] == False</filter> | |
200 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
201 <filter>'sample' in partition_type or not partition_type</filter> | |
202 <actions> | |
203 <conditional name="output_format"> | |
204 <when value="rtable"> | |
205 <action type="format"> | |
206 <option type="from_param" name="output_format" /> | |
207 </action> | |
208 </when> | |
209 <when value="csv"> | |
210 <action type="format"> | |
211 <option type="from_param" name="output_format" /> | |
212 </action> | |
213 </when> | |
214 </conditional> | |
215 </actions> | |
216 </data> | |
217 <data format="tabular" name="output_statistics_sample" label="${tool.name} on ${on_string} (output statistics sample)" > | |
218 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_per_sample_stats'] == False</filter> | |
219 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
220 <filter>'sample' in partition_type or not partition_type</filter> | |
221 <actions> | |
222 <conditional name="output_format"> | |
223 <when value="rtable"> | |
224 <action type="format"> | |
225 <option type="from_param" name="output_format" /> | |
226 </action> | |
227 </when> | |
228 <when value="csv"> | |
229 <action type="format"> | |
230 <option type="from_param" name="output_format" /> | |
231 </action> | |
232 </when> | |
233 </conditional> | |
234 </actions> | |
235 </data> | |
236 <data format="tabular" name="output_interval_summary_sample" label="${tool.name} on ${on_string} (output interval summary sample)" > | |
237 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
238 <filter>'sample' in partition_type or not partition_type</filter> | |
239 <filter>gatk_param_type['gatk_param_type_selector'] == "advanced" and len( gatk_param_type['input_interval_repeat'] )</filter> | |
240 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_interval_statistics'] == False</filter> | |
241 <actions> | |
242 <conditional name="output_format"> | |
243 <when value="rtable"> | |
244 <action type="format"> | |
245 <option type="from_param" name="output_format" /> | |
246 </action> | |
247 </when> | |
248 <when value="csv"> | |
249 <action type="format"> | |
250 <option type="from_param" name="output_format" /> | |
251 </action> | |
252 </when> | |
253 </conditional> | |
254 </actions> | |
255 </data> | |
256 <data format="tabular" name="output_interval_statistics_sample" label="${tool.name} on ${on_string} (output interval statistics sample)" > | |
257 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
258 <filter>'sample' in partition_type or not partition_type</filter> | |
259 <filter>gatk_param_type['gatk_param_type_selector'] == "advanced" and len( gatk_param_type['input_interval_repeat'] )</filter> | |
260 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_interval_statistics'] == False</filter> | |
261 <actions> | |
262 <conditional name="output_format"> | |
263 <when value="rtable"> | |
264 <action type="format"> | |
265 <option type="from_param" name="output_format" /> | |
266 </action> | |
267 </when> | |
268 <when value="csv"> | |
269 <action type="format"> | |
270 <option type="from_param" name="output_format" /> | |
271 </action> | |
272 </when> | |
273 </conditional> | |
274 </actions> | |
275 </data> | |
276 <data format="tabular" name="output_gene_summary_sample" label="${tool.name} on ${on_string} (output gene summary sample)" > | |
277 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
278 <filter>input_calculate_coverage_over_genes is not None and 'sample' in partition_type or not partition_type</filter> | |
279 <actions> | |
280 <conditional name="output_format"> | |
281 <when value="rtable"> | |
282 <action type="format"> | |
283 <option type="from_param" name="output_format" /> | |
284 </action> | |
285 </when> | |
286 <when value="csv"> | |
287 <action type="format"> | |
288 <option type="from_param" name="output_format" /> | |
289 </action> | |
290 </when> | |
291 </conditional> | |
292 </actions> | |
293 </data> | |
294 <data format="tabular" name="output_gene_statistics_sample" label="${tool.name} on ${on_string} (output gene statistics sample)" > | |
295 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
296 <filter>input_calculate_coverage_over_genes is not None and 'sample' in partition_type or not partition_type</filter> | |
297 <actions> | |
298 <conditional name="output_format"> | |
299 <when value="rtable"> | |
300 <action type="format"> | |
301 <option type="from_param" name="output_format" /> | |
302 </action> | |
303 </when> | |
304 <when value="csv"> | |
305 <action type="format"> | |
306 <option type="from_param" name="output_format" /> | |
307 </action> | |
308 </when> | |
309 </conditional> | |
310 </actions> | |
311 </data> | |
312 <data format="tabular" name="output_cumulative_coverage_counts_sample" label="${tool.name} on ${on_string} (output cumulative coverage counts sample)" > | |
313 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_depth_output_at_each_base'] == False</filter> | |
314 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
315 <filter>'sample' in partition_type or not partition_type</filter> | |
316 <actions> | |
317 <conditional name="output_format"> | |
318 <when value="rtable"> | |
319 <action type="format"> | |
320 <option type="from_param" name="output_format" /> | |
321 </action> | |
322 </when> | |
323 <when value="csv"> | |
324 <action type="format"> | |
325 <option type="from_param" name="output_format" /> | |
326 </action> | |
327 </when> | |
328 </conditional> | |
329 </actions> | |
330 </data> | |
331 <data format="tabular" name="output_cumulative_coverage_proportions_sample" label="${tool.name} on ${on_string} (output cumulative coverage proportions sample)" > | |
332 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_depth_output_at_each_base'] == False</filter> | |
333 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
334 <filter>'sample' in partition_type or not partition_type</filter> | |
335 <actions> | |
336 <conditional name="output_format"> | |
337 <when value="rtable"> | |
338 <action type="format"> | |
339 <option type="from_param" name="output_format" /> | |
340 </action> | |
341 </when> | |
342 <when value="csv"> | |
343 <action type="format"> | |
344 <option type="from_param" name="output_format" /> | |
345 </action> | |
346 </when> | |
347 </conditional> | |
348 </actions> | |
349 </data> | |
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350 |
0 | 351 <data format="tabular" name="output_summary_readgroup" label="${tool.name} on ${on_string} (output summary readgroup)" > |
352 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_per_sample_stats'] == False</filter> | |
353 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
354 <filter>'readgroup' in partition_type</filter> | |
355 <actions> | |
356 <conditional name="output_format"> | |
357 <when value="rtable"> | |
358 <action type="format"> | |
359 <option type="from_param" name="output_format" /> | |
360 </action> | |
361 </when> | |
362 <when value="csv"> | |
363 <action type="format"> | |
364 <option type="from_param" name="output_format" /> | |
365 </action> | |
366 </when> | |
367 </conditional> | |
368 </actions> | |
369 </data> | |
370 <data format="tabular" name="output_statistics_readgroup" label="${tool.name} on ${on_string} (output statistics readgroup)" > | |
371 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_per_sample_stats'] == False</filter> | |
372 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
373 <filter>'readgroup' in partition_type</filter> | |
374 <actions> | |
375 <conditional name="output_format"> | |
376 <when value="rtable"> | |
377 <action type="format"> | |
378 <option type="from_param" name="output_format" /> | |
379 </action> | |
380 </when> | |
381 <when value="csv"> | |
382 <action type="format"> | |
383 <option type="from_param" name="output_format" /> | |
384 </action> | |
385 </when> | |
386 </conditional> | |
387 </actions> | |
388 </data> | |
389 <data format="tabular" name="output_interval_summary_readgroup" label="${tool.name} on ${on_string} (output interval summary readgroup)" > | |
390 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
391 <filter>'readgroup' in partition_type</filter> | |
392 <filter>gatk_param_type['gatk_param_type_selector'] == "advanced" and len( gatk_param_type['input_interval_repeat'] )</filter> | |
393 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_interval_statistics'] == False</filter> | |
394 <actions> | |
395 <conditional name="output_format"> | |
396 <when value="rtable"> | |
397 <action type="format"> | |
398 <option type="from_param" name="output_format" /> | |
399 </action> | |
400 </when> | |
401 <when value="csv"> | |
402 <action type="format"> | |
403 <option type="from_param" name="output_format" /> | |
404 </action> | |
405 </when> | |
406 </conditional> | |
407 </actions> | |
408 </data> | |
409 <data format="tabular" name="output_interval_statistics_readgroup" label="${tool.name} on ${on_string} (output interval statistics readgroup)" > | |
410 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
411 <filter>'readgroup' in partition_type</filter> | |
412 <filter>gatk_param_type['gatk_param_type_selector'] == "advanced" and len( gatk_param_type['input_interval_repeat'] )</filter> | |
413 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_interval_statistics'] == False</filter> | |
414 <actions> | |
415 <conditional name="output_format"> | |
416 <when value="rtable"> | |
417 <action type="format"> | |
418 <option type="from_param" name="output_format" /> | |
419 </action> | |
420 </when> | |
421 <when value="csv"> | |
422 <action type="format"> | |
423 <option type="from_param" name="output_format" /> | |
424 </action> | |
425 </when> | |
426 </conditional> | |
427 </actions> | |
428 </data> | |
429 <data format="tabular" name="output_gene_summary_readgroup" label="${tool.name} on ${on_string} (output gene summary readgroup)" > | |
430 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
431 <filter>input_calculate_coverage_over_genes is not None and 'readgroup' in partition_type or not partition_type</filter> | |
432 <actions> | |
433 <conditional name="output_format"> | |
434 <when value="rtable"> | |
435 <action type="format"> | |
436 <option type="from_param" name="output_format" /> | |
437 </action> | |
438 </when> | |
439 <when value="csv"> | |
440 <action type="format"> | |
441 <option type="from_param" name="output_format" /> | |
442 </action> | |
443 </when> | |
444 </conditional> | |
445 </actions> | |
446 </data> | |
447 <data format="tabular" name="output_gene_statistics_readgroup" label="${tool.name} on ${on_string} (output gene statistics readgroup)" > | |
448 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
449 <filter>input_calculate_coverage_over_genes is not None and 'readgroup' in partition_type or not partition_type</filter> | |
450 <actions> | |
451 <conditional name="output_format"> | |
452 <when value="rtable"> | |
453 <action type="format"> | |
454 <option type="from_param" name="output_format" /> | |
455 </action> | |
456 </when> | |
457 <when value="csv"> | |
458 <action type="format"> | |
459 <option type="from_param" name="output_format" /> | |
460 </action> | |
461 </when> | |
462 </conditional> | |
463 </actions> | |
464 </data> | |
465 <data format="tabular" name="output_cumulative_coverage_counts_readgroup" label="${tool.name} on ${on_string} (output cumulative coverage counts readgroup)" > | |
466 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
467 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_depth_output_at_each_base'] == False</filter> | |
468 <filter>'readgroup' in partition_type</filter> | |
469 <actions> | |
470 <conditional name="output_format"> | |
471 <when value="rtable"> | |
472 <action type="format"> | |
473 <option type="from_param" name="output_format" /> | |
474 </action> | |
475 </when> | |
476 <when value="csv"> | |
477 <action type="format"> | |
478 <option type="from_param" name="output_format" /> | |
479 </action> | |
480 </when> | |
481 </conditional> | |
482 </actions> | |
483 </data> | |
484 <data format="tabular" name="output_cumulative_coverage_proportions_readgroup" label="${tool.name} on ${on_string} (output cumulative coverage proportions readgroup)" > | |
485 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
486 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_depth_output_at_each_base'] == False</filter> | |
487 <filter>'readgroup' in partition_type</filter> | |
488 <actions> | |
489 <conditional name="output_format"> | |
490 <when value="rtable"> | |
491 <action type="format"> | |
492 <option type="from_param" name="output_format" /> | |
493 </action> | |
494 </when> | |
495 <when value="csv"> | |
496 <action type="format"> | |
497 <option type="from_param" name="output_format" /> | |
498 </action> | |
499 </when> | |
500 </conditional> | |
501 </actions> | |
502 </data> | |
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503 |
0 | 504 <data format="tabular" name="output_summary_library" label="${tool.name} on ${on_string} (output summary library)" > |
505 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_per_sample_stats'] == False</filter> | |
506 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
507 <filter>'library' in partition_type</filter> | |
508 <actions> | |
509 <conditional name="output_format"> | |
510 <when value="rtable"> | |
511 <action type="format"> | |
512 <option type="from_param" name="output_format" /> | |
513 </action> | |
514 </when> | |
515 <when value="csv"> | |
516 <action type="format"> | |
517 <option type="from_param" name="output_format" /> | |
518 </action> | |
519 </when> | |
520 </conditional> | |
521 </actions> | |
522 </data> | |
523 <data format="tabular" name="output_statistics_library" label="${tool.name} on ${on_string} (output statistics library)" > | |
524 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_per_sample_stats'] == False</filter> | |
525 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
526 <filter>'library' in partition_type</filter> | |
527 <actions> | |
528 <conditional name="output_format"> | |
529 <when value="rtable"> | |
530 <action type="format"> | |
531 <option type="from_param" name="output_format" /> | |
532 </action> | |
533 </when> | |
534 <when value="csv"> | |
535 <action type="format"> | |
536 <option type="from_param" name="output_format" /> | |
537 </action> | |
538 </when> | |
539 </conditional> | |
540 </actions> | |
541 </data> | |
542 <data format="tabular" name="output_interval_summary_library" label="${tool.name} on ${on_string} (output interval summary library)" > | |
543 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
544 <filter>'library' in partition_type</filter> | |
545 <filter>gatk_param_type['gatk_param_type_selector'] == "advanced" and len( gatk_param_type['input_interval_repeat'] )</filter> | |
546 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_interval_statistics'] == False</filter> | |
547 <actions> | |
548 <conditional name="output_format"> | |
549 <when value="rtable"> | |
550 <action type="format"> | |
551 <option type="from_param" name="output_format" /> | |
552 </action> | |
553 </when> | |
554 <when value="csv"> | |
555 <action type="format"> | |
556 <option type="from_param" name="output_format" /> | |
557 </action> | |
558 </when> | |
559 </conditional> | |
560 </actions> | |
561 </data> | |
562 <data format="tabular" name="output_interval_statistics_library" label="${tool.name} on ${on_string} (output interval statistics library)" > | |
563 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
564 <filter>'library' in partition_type</filter> | |
565 <filter>gatk_param_type['gatk_param_type_selector'] == "advanced" and len( gatk_param_type['input_interval_repeat'] )</filter> | |
566 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_interval_statistics'] == False</filter> | |
567 <actions> | |
568 <conditional name="output_format"> | |
569 <when value="rtable"> | |
570 <action type="format"> | |
571 <option type="from_param" name="output_format" /> | |
572 </action> | |
573 </when> | |
574 <when value="csv"> | |
575 <action type="format"> | |
576 <option type="from_param" name="output_format" /> | |
577 </action> | |
578 </when> | |
579 </conditional> | |
580 </actions> | |
581 </data> | |
582 <data format="tabular" name="output_gene_summary_library" label="${tool.name} on ${on_string} (output gene summary library)" > | |
583 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
584 <filter>input_calculate_coverage_over_genes is not None and 'library' in partition_type or not partition_type</filter> | |
585 <actions> | |
586 <conditional name="output_format"> | |
587 <when value="rtable"> | |
588 <action type="format"> | |
589 <option type="from_param" name="output_format" /> | |
590 </action> | |
591 </when> | |
592 <when value="csv"> | |
593 <action type="format"> | |
594 <option type="from_param" name="output_format" /> | |
595 </action> | |
596 </when> | |
597 </conditional> | |
598 </actions> | |
599 </data> | |
600 <data format="tabular" name="output_gene_statistics_library" label="${tool.name} on ${on_string} (output gene statistics library)" > | |
601 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
602 <filter>input_calculate_coverage_over_genes is not None and 'library' in partition_type or not partition_type</filter> | |
603 <actions> | |
604 <conditional name="output_format"> | |
605 <when value="rtable"> | |
606 <action type="format"> | |
607 <option type="from_param" name="output_format" /> | |
608 </action> | |
609 </when> | |
610 <when value="csv"> | |
611 <action type="format"> | |
612 <option type="from_param" name="output_format" /> | |
613 </action> | |
614 </when> | |
615 </conditional> | |
616 </actions> | |
617 </data> | |
618 <data format="tabular" name="output_cumulative_coverage_counts_library" label="${tool.name} on ${on_string} (output cumulative coverage counts library)" > | |
619 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_depth_output_at_each_base'] == False</filter> | |
620 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
621 <filter>'library' in partition_type</filter> | |
622 <actions> | |
623 <conditional name="output_format"> | |
624 <when value="rtable"> | |
625 <action type="format"> | |
626 <option type="from_param" name="output_format" /> | |
627 </action> | |
628 </when> | |
629 <when value="csv"> | |
630 <action type="format"> | |
631 <option type="from_param" name="output_format" /> | |
632 </action> | |
633 </when> | |
634 </conditional> | |
635 </actions> | |
636 </data> | |
637 <data format="tabular" name="output_cumulative_coverage_proportions_library" label="${tool.name} on ${on_string} (output cumulative coverage proportions library)" > | |
638 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['omit_depth_output_at_each_base'] == False</filter> | |
639 <filter>analysis_param_type['analysis_param_type_selector'] == "basic" or analysis_param_type['print_bin_endpoints_and_exit'] == False</filter> | |
640 <filter>'library' in partition_type</filter> | |
641 <actions> | |
642 <conditional name="output_format"> | |
643 <when value="rtable"> | |
644 <action type="format"> | |
645 <option type="from_param" name="output_format" /> | |
646 </action> | |
647 </when> | |
648 <when value="csv"> | |
649 <action type="format"> | |
650 <option type="from_param" name="output_format" /> | |
651 </action> | |
652 </when> | |
653 </conditional> | |
654 </actions> | |
655 </data> | |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
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656 |
0 | 657 <data format="tabular" name="output_log" label="${tool.name} on ${on_string} (log)" /> |
658 </outputs> | |
659 <trackster_conf/> | |
660 <tests> | |
661 <test> | |
662 <param name="reference_source_selector" value="history" /> | |
663 <param name="ref_file" value="phiX.fasta" ftype="fasta" /> | |
664 <param name="input_bam" value="gatk/gatk_table_recalibration/gatk_table_recalibration_out_1.bam" ftype="bam" /> | |
665 <param name="input_calculate_coverage_over_genes" /> | |
666 <param name="partition_type" value="sample" /> | |
667 <param name="summary_coverage_threshold_group" value="0" /> | |
668 <param name="output_format" value="rtable" /> | |
669 <param name="gatk_param_type_selector" value="basic" /> | |
670 <param name="analysis_param_type_selector" value="basic" /> | |
671 <output name="output_per_locus_coverage" file="gatk/gatk_depth_of_coverage/gatk_depth_of_coverage_out_1_output_per_locus_coverage.tabular" /> | |
672 <output name="output_summary_sample" file="gatk/gatk_depth_of_coverage/gatk_depth_of_coverage_out_1_output_summary_sample.tabular" /> | |
673 <output name="output_statistics_sample" file="gatk/gatk_depth_of_coverage/gatk_depth_of_coverage_out_1_output_statistics_sample.tabular" /> | |
674 <output name="output_cumulative_coverage_counts_sample" file="gatk/gatk_depth_of_coverage/gatk_depth_of_coverage_out_1_output_cumulative_coverage_counts_sample.tabular" /> | |
675 <output name="output_cumulative_coverage_proportions_sample" file="gatk/gatk_depth_of_coverage/gatk_depth_of_coverage_out_1_output_output_cumulative_coverage_proportions_sample.tabular" /> | |
676 <output name="output_log" file="gatk/gatk_depth_of_coverage/gatk_depth_of_coverage_out_1.log.contains" compare="contains" /> | |
677 </test> | |
678 </tests> | |
679 <help> | |
680 **What it does** | |
681 | |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
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682 DepthOfCoverage processes a set of bam files to determine coverage at different levels of partitioning and aggregation. Coverage can be analyzed per locus, per interval, per gene, or in total; can be partitioned by sample, by read group, by technology, by center, or by library; and can be summarized by mean, median, quartiles, and/or percentage of bases covered to or beyond a threshold. Additionally, reads and bases can be filtered by mapping or base quality score. |
0 | 683 |
4 | 684 For more information on the GATK Depth of Coverage, see this `tool specific page <http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_gatk_walkers_coverage_DepthOfCoverage.html>`_. |
0 | 685 |
686 To learn about best practices for variant detection using GATK, see this `overview <http://www.broadinstitute.org/gatk/guide/topic?name=best-practices>`_. | |
687 | |
688 If you encounter errors, please view the `GATK FAQ <http://www.broadinstitute.org/gatk/guide/topic?name=faqs>`_. | |
689 | |
690 ------ | |
691 | |
692 **Inputs** | |
693 | |
694 GenomeAnalysisTK: DepthOfCoverage accepts aligned BAM input files. | |
695 | |
696 | |
697 **Outputs** | |
698 | |
699 The output is in various table formats. | |
700 | |
701 | |
702 Go `here <http://www.broadinstitute.org/gatk/guide/topic?name=intro>`_ for details on GATK file formats. | |
703 | |
704 ------- | |
705 | |
706 **Settings**:: | |
707 | |
708 calculateCoverageOverGenes File NA Calculate the coverage statistics over this list of genes. Currently accepts RefSeq. | |
709 ignoreDeletionSites boolean false Ignore sites consisting only of deletions | |
710 includeDeletions boolean false Include information on deletions | |
711 maxBaseQuality byte 127 Maximum quality of bases to count towards depth. Defaults to 127 (Byte.MAX_VALUE). | |
712 maxMappingQuality int 2147483647 Maximum mapping quality of reads to count towards depth. Defaults to 2^31-1 (Integer.MAX_VALUE). | |
713 minBaseQuality byte -1 Minimum quality of bases to count towards depth. Defaults to -1. | |
714 minMappingQuality int -1 Minimum mapping quality of reads to count towards depth. Defaults to -1. | |
715 nBins int 499 Number of bins to use for granular binning | |
716 omitDepthOutputAtEachBase boolean false Will omit the output of the depth of coverage at each base, which should result in speedup | |
717 omitIntervalStatistics boolean false Will omit the per-interval statistics section, which should result in speedup | |
718 omitLocusTable boolean false Will not calculate the per-sample per-depth counts of loci, which should result in speedup | |
719 omitPerSampleStats boolean false Omits the summary files per-sample. These statistics are still calculated, so this argument will not improve runtime. | |
720 outputFormat String rtable the format of the output file (e.g. csv, table, rtable); defaults to r-readable table | |
721 partitionType Set[Partition] [sample] Partition type for depth of coverage. Defaults to sample. Can be any combination of sample, readgroup, library. | |
722 printBaseCounts boolean false Will add base counts to per-locus output. | |
723 printBinEndpointsAndExit boolean false Prints the bin values and exits immediately. Use to calibrate what bins you want before running on data. | |
724 start int 1 Starting (left endpoint) for granular binning | |
725 stop int 500 Ending (right endpoint) for granular binning | |
726 summaryCoverageThreshold int[] [15] for summary file outputs, report the % of bases coverd to >= this number. Defaults to 15; can take multiple arguments. | |
727 | |
728 @CITATION_SECTION@ | |
729 </help> | |
4 | 730 <expand macro="citations" /> |
0 | 731 </tool> |