Mercurial > repos > iuc > gatk2
annotate indel_realigner.xml @ 6:35c00763cb5c draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
| author | iuc |
|---|---|
| date | Mon, 04 Jun 2018 05:38:15 -0400 |
| parents | f244b8209eb8 |
| children |
| rev | line source |
|---|---|
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6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
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1 <tool id="gatk2_indel_realigner" name="Indel Realigner" version="@VERSION@.1"> |
| 0 | 2 <description>- perform local realignment</description> |
| 3 <macros> | |
| 4 <import>gatk2_macros.xml</import> | |
| 5 </macros> | |
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35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
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6 <expand macro="requirements" /> |
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35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
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7 <expand macro="version_command" /> |
| 0 | 8 <command interpreter="python"> |
| 9 gatk2_wrapper.py | |
| 10 --stdout "${output_log}" | |
| 11 -d "-I" "${reference_source.input_bam}" "${reference_source.input_bam.ext}" "gatk_input" | |
| 12 #if str( $reference_source.input_bam.metadata.bam_index ) != "None": | |
| 13 -d "" "${reference_source.input_bam.metadata.bam_index}" "bam_index" "gatk_input" ##hardcode galaxy ext type as bam_index | |
| 14 #end if | |
| 15 -p ' | |
| 16 @JAR_PATH@ | |
| 17 -T "IndelRealigner" | |
| 18 -o "${output_bam}" | |
| 19 | |
| 20 \$GATK2_SITE_OPTIONS | |
| 21 | |
| 22 ## according to http://www.broadinstitute.org/gatk/guide/article?id=1975 | |
| 23 --num_cpu_threads_per_data_thread 1 | |
| 24 | |
| 25 #if $reference_source.reference_source_selector != "history": | |
| 26 -R "${reference_source.ref_file.fields.path}" | |
| 27 #end if | |
| 28 -LOD "${lod_threshold}" | |
| 29 ${knowns_only} | |
| 30 ' | |
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35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
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31 |
| 0 | 32 #set $rod_binding_names = dict() |
| 33 #for $rod_binding in $rod_bind: | |
| 34 #if str( $rod_binding.rod_bind_type.rod_bind_type_selector ) == 'custom': | |
| 35 #set $rod_bind_name = $rod_binding.rod_bind_type.custom_rod_name | |
| 36 #else | |
| 37 #set $rod_bind_name = $rod_binding.rod_bind_type.rod_bind_type_selector | |
| 38 #end if | |
| 39 #set $rod_binding_names[$rod_bind_name] = $rod_binding_names.get( $rod_bind_name, -1 ) + 1 | |
| 40 -d "-known:${rod_bind_name},%(file_type)s" "${rod_binding.rod_bind_type.input_rod}" "${rod_binding.rod_bind_type.input_rod.ext}" "input_${rod_bind_name}_${rod_binding_names[$rod_bind_name]}" | |
| 41 #end for | |
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6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
42 |
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35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
43 $allow_n_cigar_reads |
| 0 | 44 #include source=$standard_gatk_options# |
| 45 ##start analysis specific options | |
| 46 -d "-targetIntervals" "${target_intervals}" "${target_intervals.ext}" "gatk_target_intervals" | |
| 47 -p ' | |
| 48 --disable_bam_indexing | |
| 49 ' | |
| 50 #if $analysis_param_type.analysis_param_type_selector == "advanced": | |
| 51 -p ' | |
| 52 --entropyThreshold "${analysis_param_type.entropy_threshold}" | |
| 53 ${analysis_param_type.simplify_bam} | |
| 54 --consensusDeterminationModel "${analysis_param_type.consensus_determination_model}" | |
| 55 --maxIsizeForMovement "${analysis_param_type.max_insert_size_for_movement}" | |
| 56 --maxPositionalMoveAllowed "${analysis_param_type.max_positional_move_allowed}" | |
| 57 --maxConsensuses "${analysis_param_type.max_consensuses}" | |
| 58 --maxReadsForConsensuses "${analysis_param_type.max_reads_for_consensuses}" | |
| 59 --maxReadsForRealignment "${analysis_param_type.max_reads_for_realignment}" | |
| 60 ${analysis_param_type.no_original_alignment_tags} | |
| 61 ' | |
| 62 #end if | |
| 63 </command> | |
| 64 <inputs> | |
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6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
65 |
| 0 | 66 <conditional name="reference_source"> |
| 67 <expand macro="reference_source_selector_param" /> | |
| 68 <when value="cached"> | |
| 69 <param name="input_bam" type="data" format="bam" label="BAM file" help="-I,--input_file &lt;input_file&gt;"> | |
| 70 <validator type="unspecified_build" /> | |
| 71 <validator type="dataset_metadata_in_data_table" table_name="gatk2_picard_indexes" metadata_name="dbkey" metadata_column="dbkey" message="Sequences are not currently available for the specified build." /> <!-- fixme!!! this needs to be a select --> | |
| 72 </param> | |
| 73 <param name="ref_file" type="select" label="Using reference genome" help="-R,--reference_sequence &lt;reference_sequence&gt;" > | |
| 74 <options from_data_table="gatk2_picard_indexes"> | |
| 75 <filter type="data_meta" key="dbkey" ref="input_bam" column="dbkey"/> | |
| 76 </options> | |
| 77 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> | |
| 78 </param> | |
| 79 </when> | |
| 80 <when value="history"> | |
| 81 <param name="input_bam" type="data" format="bam" label="BAM file" help="-I,--input_file &lt;input_file&gt;" /> | |
| 82 <param name="ref_file" type="data" format="fasta" label="Using reference file" help="-R,--reference_sequence &lt;reference_sequence&gt;"> | |
| 83 <options> | |
| 84 <filter type="data_meta" key="dbkey" ref="input_bam" /> | |
| 85 </options> | |
| 86 </param> | |
| 87 </when> | |
| 88 </conditional> | |
| 89 <param name="target_intervals" type="data" format="gatk_interval,bed,picard_interval_list" label="Restrict realignment to provided intervals" help="-targetIntervals,--targetIntervals &lt;targetIntervals&gt;" /> | |
| 90 <repeat name="rod_bind" title="Known Variants" help="Using data sets of known variants (-known,--knownAlleles &lt;knownAlleles&gt;)"> | |
| 91 <conditional name="rod_bind_type"> | |
| 92 <param name="rod_bind_type_selector" type="select" label="Variant Type"> | |
| 93 <option value="dbsnp" selected="True">dbSNP</option> | |
| 94 <option value="snps">SNPs</option> | |
| 95 <option value="indels">INDELs</option> | |
| 96 <option value="custom">Custom</option> | |
| 97 </param> | |
| 98 <when value="dbsnp"> | |
| 99 <param name="input_rod" type="data" format="vcf" label="Variant file (VCF format)" /> | |
| 100 </when> | |
| 101 <when value="snps"> | |
| 102 <param name="input_rod" type="data" format="vcf" label="Variant file (VCF format)" /> | |
| 103 </when> | |
| 104 <when value="indels"> | |
| 105 <param name="input_rod" type="data" format="vcf" label="Variant file (VCF format)" /> | |
| 106 </when> | |
| 107 <when value="custom"> | |
| 108 <param name="custom_rod_name" type="text" value="Unknown" label="Customer's variant file"/> | |
| 109 <param name="input_rod" type="data" format="vcf" label="Variant file (VCF format)" /> | |
| 110 </when> | |
| 111 </conditional> | |
| 112 </repeat> | |
| 113 <param name="lod_threshold" type="float" value="5.0" label="LOD threshold above which the realigner will proceed to realign" help="-LOD,--LODThresholdForCleaning &lt;LODThresholdForCleaning&gt;" /> | |
| 114 <param name="knowns_only" type="boolean" checked="False" truevalue="-knownsOnly" falsevalue="" label="Use only known indels provided as RODs" help="-knownsOnly"/> | |
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6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
115 |
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35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
116 <expand macro="allow_n_cigar_reads" /> |
| 0 | 117 <expand macro="gatk_param_type_conditional" /> |
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6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
118 |
| 0 | 119 <expand macro="analysis_type_conditional"> |
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6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
120 |
| 0 | 121 <param name="entropy_threshold" type="float" value="0.15" label="percentage of mismatching base quality scores at a position to be considered having high entropy" help="-entropy,--entropyThreshold &lt;entropyThreshold&gt;" /> |
| 122 <param name="simplify_bam" type="boolean" checked="False" truevalue="-simplifyBAM" falsevalue="" label="Simplify BAM" help="-simplifyBAM,--simplifyBAM"/> | |
| 123 <param name="consensus_determination_model" type="select" label="Consensus Determination Model" help="-model,--consensusDeterminationModel &lt;consensusDeterminationModel&gt;"> | |
| 124 <option value="KNOWNS_ONLY">KNOWNS_ONLY</option> | |
| 125 <option value="USE_READS" selected="True">USE_READS</option> | |
| 126 <option value="USE_SW">USE_SW</option> | |
| 127 </param> | |
| 128 <param name="max_insert_size_for_movement" type="integer" value="3000" label="Maximum insert size of read pairs that we attempt to realign" help="-maxIsize,--maxIsizeForMovement &lt;maxIsizeForMovement&gt;" /> | |
| 129 <param name="max_positional_move_allowed" type="integer" value="200" label="Maximum positional move in basepairs that a read can be adjusted during realignment" help="-maxPosMove,--maxPositionalMoveAllowed &lt;maxPositionalMoveAllowed&gt;" /> | |
| 130 <param name="max_consensuses" type="integer" value="30" label="Max alternate consensuses to try" help="-maxConsensuses,--maxConsensuses &lt;maxConsensuses&gt;" /> | |
| 131 <param name="max_reads_for_consensuses" type="integer" value="120" label="Max reads (chosen randomly) used for finding the potential alternate consensuses" help="-greedy,--maxReadsForConsensuses &lt;maxReadsForConsensuses&gt;" /> | |
| 132 <param name="max_reads_for_realignment" type="integer" value="20000" label="Max reads allowed at an interval for realignment" help="-maxReads,--maxReadsForRealignment &lt;maxReadsForRealignment&gt;" /> | |
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6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
133 <param name="no_original_alignment_tags" type="boolean" checked="False" truevalue="--noOriginalAlignmentTags" falsevalue="" label="Don't output the original cigar or alignment start tags for each realigned read in the output bam" help="-noTags,--noOriginalAlignmentTags"/> |
| 0 | 134 </expand> |
| 135 </inputs> | |
| 136 <outputs> | |
| 137 <data format="bam" name="output_bam" label="${tool.name} on ${on_string} (BAM)" /> | |
| 138 <data format="txt" name="output_log" label="${tool.name} on ${on_string} (log)" /> | |
| 139 </outputs> | |
| 140 <tests> | |
| 141 <test> | |
| 142 <param name="reference_source_selector" value="history" /> | |
| 143 <param name="ref_file" value="phiX.fasta" ftype="fasta" /> | |
| 144 <param name="target_intervals" value="gatk/gatk_realigner_target_creator/gatk_realigner_target_creator_out_1.gatk_interval" ftype="gatk_interval" /> | |
| 145 <param name="input_bam" value="gatk/fake_phiX_reads_1.bam" ftype="bam" /> | |
| 146 <param name="rod_bind_type_selector" value="snps" /> | |
| 147 <param name="input_rod" value="gatk/fake_phiX_variant_locations.vcf" ftype="vcf" /> | |
| 148 <param name="lod_threshold" value="5.0" /> | |
| 149 <param name="knowns_only" /> | |
| 150 <param name="gatk_param_type_selector" value="basic" /> | |
| 151 <param name="analysis_param_type_selector" value="advanced" /> | |
| 152 <param name="entropy_threshold" value="0.15" /> | |
| 153 <param name="simplify_bam" /> | |
| 154 <param name="consensus_determination_model" value="USE_SW" /> | |
| 155 <param name="max_insert_size_for_movement" value="3000" /> | |
| 156 <param name="max_positional_move_allowed" value="200" /> | |
| 157 <param name="max_consensuses" value="30" /> | |
| 158 <param name="max_reads_for_consensuses" value="120" /> | |
| 159 <param name="max_reads_for_realignment" value="20000" /> | |
| 160 <param name="no_original_alignment_tags" /> | |
|
6
35c00763cb5c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gatk2 commit cf399638ebca4250bcc15f468238a9964de97b33
iuc
parents:
4
diff
changeset
|
161 <output name="output_bam" file="gatk/gatk_indel_realigner/gatk_indel_realigner_out_1.bam" ftype="bam" lines_diff="2" /> |
| 0 | 162 <output name="output_log" file="gatk/gatk_indel_realigner/gatk_indel_realigner_out_1.log.contains" compare="contains" /> |
| 163 </test> | |
| 164 </tests> | |
| 165 <help> | |
| 166 **What it does** | |
| 167 | |
| 168 Performs local realignment of reads based on misalignments due to the presence of indels. Unlike most mappers, this walker uses the full alignment context to determine whether an appropriate alternate reference (i.e. indel) exists and updates SAMRecords accordingly. | |
| 169 | |
| 170 For more information on local realignment around indels using the GATK, see this `tool specific page <http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_gatk_walkers_indels_IndelRealigner.html>`_. | |
| 171 | |
| 172 To learn about best practices for variant detection using GATK, see this `overview <http://www.broadinstitute.org/gatk/guide/topic?name=best-practices>`_. | |
| 173 | |
| 174 If you encounter errors, please view the `GATK FAQ <http://www.broadinstitute.org/gatk/guide/topic?name=faqs>`_. | |
| 175 | |
| 176 ------ | |
| 177 | |
| 178 **Inputs** | |
| 179 | |
| 180 GenomeAnalysisTK: IndelRealigner accepts an aligned BAM and a list of intervals to realign as input files. | |
| 181 | |
| 182 | |
| 183 **Outputs** | |
| 184 | |
| 185 The output is in the BAM format. | |
| 186 | |
| 187 | |
| 188 Go `here <http://www.broadinstitute.org/gatk/guide/topic?name=intro>`_ for details on GATK file formats. | |
| 189 | |
| 190 ------- | |
| 191 | |
| 192 **Settings**:: | |
| 193 | |
| 194 targetIntervals intervals file output from RealignerTargetCreator | |
| 195 LODThresholdForCleaning LOD threshold above which the cleaner will clean | |
| 196 entropyThreshold percentage of mismatches at a locus to be considered having high entropy | |
| 197 out Output bam | |
| 198 bam_compression Compression level to use for writing BAM files | |
| 199 disable_bam_indexing Turn off on-the-fly creation of indices for output BAM files. | |
| 200 simplifyBAM If provided, output BAM files will be simplified to include just key reads for downstream variation discovery analyses (removing duplicates, PF-, non-primary reads), as well stripping all extended tags from the kept reads except the read group identifier | |
| 201 useOnlyKnownIndels Don't run 'Smith-Waterman' to generate alternate consenses; use only known indels provided as RODs for constructing the alternate references. | |
| 202 maxReadsInMemory max reads allowed to be kept in memory at a time by the SAMFileWriter. Keep it low to minimize memory consumption (but the tool may skip realignment on regions with too much coverage. If it is too low, it may generate errors during realignment); keep it high to maximize realignment (but make sure to give Java enough memory). | |
| 203 maxIsizeForMovement maximum insert size of read pairs that we attempt to realign | |
| 204 maxPositionalMoveAllowed maximum positional move in basepairs that a read can be adjusted during realignment | |
| 205 maxConsensuses max alternate consensuses to try (necessary to improve performance in deep coverage) | |
| 206 maxReadsForConsensuses max reads used for finding the alternate consensuses (necessary to improve performance in deep coverage) | |
| 207 maxReadsForRealignment max reads allowed at an interval for realignment; if this value is exceeded, realignment is not attempted and the reads are passed to the output file(s) as-is | |
| 208 noOriginalAlignmentTags Don't output the original cigar or alignment start tags for each realigned read in the output bam. | |
| 209 | |
| 210 @CITATION_SECTION@ | |
| 211 </help> | |
| 4 | 212 <expand macro="citations" /> |
| 0 | 213 </tool> |