# HG changeset patch # User iuc # Date 1572464039 14400 # Node ID f41a1e03538baf1b58a69d21fd669b47fa31ea59 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/gatk4 commit f9d04b348a43a799ab1624d3a7b211aab55ae522" diff -r 000000000000 -r f41a1e03538b gatk4_Mutect2.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/gatk4_Mutect2.xml Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,758 @@ + + - Call somatic SNVs and indels via local assembly of haplotypes + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + str(outputs['output_parameters']) == 'yes' and outputs['debug_activity'] + + + str(outputs['output_parameters']) == 'yes' and outputs['debug_assembly'] + + + str(outputs['output_parameters']) == 'yes' and outputs['debug_bam'] + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + `__) +with the assembly-based machinery of +`HaplotypeCaller `__. + +This tool is featured in the *Somatic Short Mutation calling Best +Practice Workflow*. See +`Tutorial#11136 `__ +for a step-by-step description of the workflow and +`Article#11127 `__ +for an overview of what traditional somatic calling entails. For the +latest pipeline scripts, see the `Mutect2 WDL scripts +directory `__. +Although we present the tool for somatic calling, it may apply to other +contexts, such as mitochondrial variant calling. + +Usage examples +~~~~~~~~~~~~~~ + +Example commands show how to run Mutect2 for typical scenarios. The two +modes are (i) *somatic mode* where a tumor sample is matched with a +normal sample in analysis and (ii) *tumor-only mode* where a single +sample's alignment data undergoes analysis. + +(i) Tumor with matched normal +^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ + +Given a matched normal, Mutect2 is designed to call somatic variants +only. The tool includes logic to skip emitting variants that are clearly +present in the germline based on provided evidence, e.g. in the matched +normal. This is done at an early stage to avoid spending computational +resources on germline events. If the variant's germline status is +borderline, then Mutect2 will emit the variant to the callset for +subsequent filtering and review. + +:: + + gatk Mutect2 \ + -R reference.fa \ + -I tumor.bam \ + -tumor tumor_sample_name \ + -I normal.bam \ + -normal normal_sample_name \ + --germline-resource af-only-gnomad.vcf.gz \ + --af-of-alleles-not-in-resource 0.00003125 \ + --panel-of-normals pon.vcf.gz \ + -O somatic.vcf.gz + + +The --af-of-alleles-not-in-resource argument value should match +expectations for alleles not found in the provided germline resource. +Note the tool does not require a germline resource nor a panel of +normals (PoN) to run. The tool prefilters sites for the matched normal +and the PoN. For the germline resource, the tool prefilters on the +allele. Below is an excerpt of a known variants resource with population +allele frequencies + +:: + + #CHROM POS ID REF ALT QUAL FILTER INFO + 1 10067 . T TAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC 30.35 PASS AC=3;AF=7.384E-5 + 1 10108 . CAACCCT C 46514.32 PASS AC=6;AF=1.525E-4 + 1 10109 . AACCCTAACCCT AAACCCT,* 89837.27 PASS AC=48,5;AF=0.001223,1.273E-4 + 1 10114 . TAACCCTAACCCTAACCCTAACCCTAACCCTAACCCCTAACCCTAACCCTAACCCTAACCCTAACCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCCTAACCCTAACCCTAAACCCTA *,CAACCCTAACCCTAACCCTAACCCTAACCCTAACCCCTAACCCTAACCCTAACCCTAACCCTAACCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCCTAACCCTAACCCTAAACCCTA,T 36728.97 PASS AC=55,9,1;AF=0.001373,2.246E-4,2.496E-5 + 1 10119 . CT C,* 251.23 PASS AC=5,1;AF=1.249E-4,2.498E-5 + 1 10120 . TA CA,* 14928.74 PASS AC=10,6;AF=2.5E-4,1.5E-4 + 1 10128 . ACCCTAACCCTAACCCTAAC A,* 285.71 PASS AC=3,1;AF=7.58E-5,2.527E-5 + 1 10131 . CT C,* 378.93 PASS AC=7,5;AF=1.765E-4,1.261E-4 + 1 10132 . TAACCC *,T 18025.11 PASS AC=12,2;AF=3.03E-4,5.049E-5 + + +(ii) Tumor-only mode +^^^^^^^^^^^^^^^^^^^^ + +This mode runs on a single sample, e.g. single tumor or single normal +sample. To create a PoN, call on each normal sample in this mode, then +use CreateSomaticPanelOfNormals to generate the PoN. + +:: + + gatk Mutect2 \ + -R reference.fa \ + -I sample.bam \ + -tumor sample_name \ + -O single_sample.vcf.gz + + +Further points of interest +~~~~~~~~~~~~~~~~~~~~~~~~~~ + +Additional parameters that factor towards filtering, including +normal-artifact-lod (default threshold 0.0) and tumor-lod (default +threshold 5.3), are available in FilterMutectCalls. While the tool +calculates normal-lod assuming a diploid genotype, it calculates +normal-artifact-lod with the same approach it uses for tumor-lod, i.e. +with a variable ploidy assumption. + +- If the normal artifact log odds becomes large, then FilterMutectCalls applies the artifact-in-normal filter. For matched normal samples with tumor contamination, consider increasing the normal-artifact-lod threshold. + +- The tumor log odds, which is calculated independently of any matched normal, determines whether to filter a tumor variant. Variants with tumor LODs exceeding the threshold pass filtering. + + +If a variant is absent from a given germline resource, then the value +for --af-of-alleles-not-in-resource applies. For example, gnomAD's +16,000 samples (~32,000 homologs per locus) becomes a probability of one +in 32,000 or less. Thus, an allele's absence from the germline resource +becomes evidence that it is not a germline variant. + +Caveats +~~~~~~~ + +Although GATK4 Mutect2 accomodates varying coverage depths, further +optimization of parameters may improve calling for extreme high depths, +e.g. 1000X. +]]> + + + + \ No newline at end of file diff -r 000000000000 -r f41a1e03538b macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,710 @@ + + + 4.1.4.0 + @VERSION@+galaxy + + + + gatk4 + tabix + samtools + + + + + + + gatk SortSam --version 2>&1 | grep Version | cut -d ':' -f 2 + + + + [[ -z \$_JAVA_OPTIONS ]] && export JAVA_OPTS=\$_JAVA_OPTIONS && gatk + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + + + + + + + + + + + + + + + + + + +
+
+ + + + + + + + + + not gzipped_output + + + gzipped_output + + + + + + + + + + + + picard_adv['CREATE_MD5_FILE'] + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 10.1101/gr.107524.110 + 10.1038/ng.806 + 10.1002/0471250953.bi1110s43 + 10.1101/201178 + + + +
diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-in1.bam Binary file test-data/Mutect2-in1.bam has changed diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-in2.bam Binary file test-data/Mutect2-in2.bam has changed diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-in2.dict --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/Mutect2-in2.dict Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,2 @@ +@HD VN:1.6 +@SQ SN:K03455 LN:9719 M5:c66838498aab02f786a6ec2f2d62cdc1 UR:file:/home/inithello/github-repos/tools-iuc/tools/gatk4/test-data/../input-data/reference.fasta diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-in3.bam Binary file test-data/Mutect2-in3.bam has changed diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-in4.bam Binary file test-data/Mutect2-in4.bam has changed diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-in5.bam Binary file test-data/Mutect2-in5.bam has changed diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-out1.vcf --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/Mutect2-out1.vcf Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,91 @@ +##fileformat=VCFv4.2 +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##GATKCommandLine= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##MutectVersion=2.2 +##contig= +##filtering_status=Warning: unfiltered Mutect 2 calls. Please run FilterMutectCalls to remove false positives. +##source=Mutect2 +##tumor_sample=SRR8525881 +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SRR8525881 +K03455 4098 . T C . . DP=1;ECNT=12;MBQ=0,34;MFRL=0,158;MMQ=60,60;MPOS=3;POPAF=7.30;TLOD=3.58 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,1:0.667:1:0,0:0,1:0,0,1,0 +K03455 4145 . T C . . DP=4;ECNT=12;MBQ=0,27;MFRL=0,242;MMQ=60,60;MPOS=39;POPAF=7.30;TLOD=17.40 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,4:0.800:4:0,1:0,3:0,0,3,1 +K03455 4190 . A G . . DP=14;ECNT=12;MBQ=38,38;MFRL=290,278;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=50.12 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:2,12:0.786:14:0,4:1,8:0|1:4190_A_G:4190:2,0,10,2 +K03455 4209 . GC AC,AA . . DP=18;ECNT=12;MBQ=37,35,35;MFRL=200,295,290;MMQ=60,60,60;MPOS=10,43;POPAF=7.30,7.30;TLOD=13.72,52.71 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:1,4,13:0.263,0.632:18:1,2,4:0,2,9:1,0,15,2 +K03455 4233 . T C . . DP=24;ECNT=12;MBQ=38,39;MFRL=252,263;MMQ=60,60;MPOS=26;POPAF=7.30;TLOD=16.13 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 1|0:19,5:0.261:24:7,2:11,3:1|0:4190_A_G:4190:16,3,5,0 +K03455 4250 . G A . . DP=36;ECNT=12;MBQ=37,37;MFRL=273,201;MMQ=60,60;MPOS=16;POPAF=7.30;TLOD=4.14 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:33,2:0.088:35:15,2:16,0:29,4,2,0 +K03455 4259 . T A . . DP=40;ECNT=12;MBQ=35,35;MFRL=254,276;MMQ=60,60;MPOS=35;POPAF=7.30;TLOD=134.66 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:7,32:0.784:39:3,15:4,13:0|1:4259_T_A:4259:7,0,27,5 +K03455 4262 . A T . . DP=40;ECNT=12;MBQ=37,37;MFRL=276,254;MMQ=60,60;MPOS=48;POPAF=7.30;TLOD=23.11 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 1|0:32,7:0.216:39:16,3:13,4:1|0:4259_T_A:4259:27,5,7,0 +K03455 4268 . G A . . DP=40;ECNT=12;MBQ=34,37;MFRL=252,273;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=47.57 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:27,13:0.368:40:11,7:13,5:22,5,13,0 +K03455 4271 . A G . . DP=43;ECNT=12;MBQ=37,33;MFRL=250,287;MMQ=60,60;MPOS=27;POPAF=7.30;TLOD=28.52 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:34,9:0.244:43:14,7:15,2:29,5,9,0 +K03455 4277 . C T . . DP=46;ECNT=12;MBQ=37,37;MFRL=244,276;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=148.83 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:10,36:0.767:46:3,18:4,16:0|1:4259_T_A:4259:9,1,31,5 +K03455 4279 . GT AC,AT . . DP=47;ECNT=12;MBQ=37,35,36;MFRL=263,252,202;MMQ=60,60,60;MPOS=34,33;POPAF=7.30,7.30;TLOD=60.64,24.20 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:17,21,9:0.421,0.201:47:8,8,5:7,9,4:16,1,25,5 +K03455 4310 . C T . . DP=39;ECNT=15;MBQ=20,18;MFRL=229,290;MMQ=60,60;MPOS=18;POPAF=7.30;TLOD=7.48 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:34,4:0.141:38:18,0:12,2:21,13,1,3 +K03455 4313 . G A . . DP=37;ECNT=15;MBQ=20,32;MFRL=229,273;MMQ=60,60;MPOS=69;POPAF=7.30;TLOD=12.35 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:32,5:0.143:37:12,3:12,1:17,15,4,1 +K03455 4320 . G A . . DP=38;ECNT=15;MBQ=20,20;MFRL=252,263;MMQ=60,60;MPOS=25;POPAF=7.30;TLOD=26.00 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:31,7:0.237:38:16,1:12,3:17,14,3,4 +K03455 4330 . A G . . DP=42;ECNT=15;MBQ=20,38;MFRL=252,276;MMQ=60,60;MPOS=60;POPAF=7.30;TLOD=13.81 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:37,4:0.125:41:16,3:17,0:19,18,3,1 +K03455 4334 . A G . . DP=43;ECNT=15;MBQ=32,36;MFRL=263,254;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=14.62 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:38,5:0.150:43:17,2:18,3:21,17,1,4 +K03455 4340 . A G . . DP=45;ECNT=15;MBQ=37,20;MFRL=263,252;MMQ=60,60;MPOS=37;POPAF=7.30;TLOD=109.80 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:17,28:0.586:45:8,13:9,11:0|1:4340_A_G:4340:9,8,13,15 +K03455 4343 . C A . . DP=43;ECNT=15;MBQ=33,20;MFRL=263,229;MMQ=60,60;MPOS=40;POPAF=7.30;TLOD=109.80 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:17,26:0.586:43:7,12:6,10:0|1:4340_A_G:4340:9,8,11,15 +K03455 4345 . GC AT . . DP=47;ECNT=15;MBQ=20,37;MFRL=204,254;MMQ=60,60;MPOS=16;POPAF=7.30;TLOD=44.93 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:34,13:0.302:47:12,4:14,6:16,18,6,7 +K03455 4346 . C T . . DP=46;ECNT=15;MBQ=20,26;MFRL=197,276;MMQ=60,60;MPOS=44;POPAF=7.30;TLOD=13.91 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:31,4:0.146:35:11,2:10,0:14,17,3,1 +K03455 4358 . T C . . DP=45;ECNT=15;MBQ=20,35;MFRL=206,254;MMQ=60,60;MPOS=29;POPAF=7.30;TLOD=17.97 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:37,7:0.171:44:13,4:19,3:16,21,2,5 +K03455 4361 . GCT ACT,G . . DP=47;ECNT=15;MBQ=36,20,35;MFRL=237,208,247;MMQ=60,60,60;MPOS=32,32;POPAF=7.30,7.30;TLOD=101.55,49.01 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:6,28,13:0.573,0.289:47:3,9,4:2,13,7:4,2,16,25 +K03455 4364 . A AAG . . DP=49;ECNT=15;MBQ=33,35;MFRL=208,247;MMQ=60,60;MPOS=35;POPAF=7.30;TLOD=48.10 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:34,13:0.295:47:15,4:17,7:15,19,5,8 +K03455 4370 . A G . . DP=51;ECNT=15;MBQ=20,37;MFRL=197,291;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=64.46 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:30,20:0.466:50:10,8:13,12:13,17,7,13 +K03455 4376 . C T . . DP=53;ECNT=15;MBQ=29,25;MFRL=231,244;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=80.90 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:28,24:0.446:52:8,10:16,12:9,19,11,13 +K03455 4397 . T C . . DP=57;ECNT=15;MBQ=34,37;MFRL=291,247;MMQ=60,60;MPOS=44;POPAF=7.30;TLOD=66.78 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:32,19:0.381:51:9,8:17,9:15,17,9,10 +K03455 4416 . C T . . DP=65;ECNT=21;MBQ=37,37;MFRL=270,310;MMQ=60,60;MPOS=47;POPAF=7.30;TLOD=33.57 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:54,11:0.193:65:21,4:31,4:22,32,6,5 +K03455 4418 . A G . . DP=64;ECNT=21;MBQ=37,37;MFRL=287,229;MMQ=60,60;MPOS=45;POPAF=7.30;TLOD=6.89 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:61,3:0.066:64:24,3:35,0:26,35,1,2 +K03455 4421 . T C . . DP=64;ECNT=21;MBQ=35,38;MFRL=259,291;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=140.93 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:28,36:0.574:64:15,13:10,21:11,17,16,20 +K03455 4424 . T C . . DP=62;ECNT=21;MBQ=37,38;MFRL=251,291;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=104.68 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:32,29:0.498:61:14,9:12,17:12,20,14,15 +K03455 4430 . T C . . DP=62;ECNT=21;MBQ=37,38;MFRL=284,265;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=3.90 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:60,2:0.051:62:24,2:31,0:25,35,1,1 +K03455 4439 . A G . . DP=54;ECNT=21;MBQ=38,35;MFRL=291,241;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=18.97 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:48,6:0.115:54:21,2:24,4:21,27,2,4 +K03455 4442 . AG GA . . DP=53;ECNT=21;MBQ=38,33;MFRL=290,209;MMQ=60,60;MPOS=27;POPAF=7.30;TLOD=18.54 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:45,7:0.159:52:18,2:23,5:19,26,3,4 +K03455 4443 . G A . . DP=53;ECNT=21;MBQ=37,38;MFRL=282,297;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=75.44 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:26,22:0.454:48:12,9:10,10:10,16,11,11 +K03455 4448 . C A . . DP=51;ECNT=21;MBQ=38,20;MFRL=290,126;MMQ=60,60;MPOS=25;POPAF=7.30;TLOD=9.07 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:47,3:0.062:50:18,0:25,2:20,27,1,2 +K03455 4449 . C T . . DP=49;ECNT=21;MBQ=37,38;MFRL=209,323;MMQ=60,60;MPOS=37;POPAF=7.30;TLOD=90.60 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:25,24:0.511:49:11,10:13,13:11,14,11,13 +K03455 4460 . T C . . DP=46;ECNT=21;MBQ=37,37;MFRL=284,329;MMQ=60,60;MPOS=18;POPAF=7.30;TLOD=18.65 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:40,6:0.159:46:15,2:22,3:20,20,2,4 +K03455 4465 . T TGGCC . . DP=45;ECNT=21;MBQ=37,35;MFRL=310,176;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=26.99 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:35,8:0.170:43:12,6:21,1:17,18,4,4 +K03455 4466 . A G,AGTG . . DP=44;ECNT=21;MBQ=38,38,37;MFRL=322,227,176;MMQ=60,60,60;MPOS=29,22;POPAF=7.30,7.30;TLOD=12.11,28.32 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:30,4,8:0.136,0.190:42:10,1,6:16,3,1:15,15,5,7 +K03455 4478 . T C . . DP=33;ECNT=21;MBQ=37,33;MFRL=323,206;MMQ=60,60;MPOS=18;POPAF=7.30;TLOD=40.91 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:23,10:0.303:33:7,6:15,4:12,11,4,6 +K03455 4505 . A G . . DP=19;ECNT=21;MBQ=39,38;MFRL=394,323;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=67.71 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:3,16:0.810:19:2,5:1,8:2,1,11,5 +K03455 4508 . A T . . DP=17;ECNT=21;MBQ=39,35;MFRL=341,335;MMQ=60,60;MPOS=31;POPAF=7.30;TLOD=53.29 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:4,13:0.737:17:2,5:2,8:2,2,10,3 +K03455 4511 . G A . . DP=17;ECNT=21;MBQ=37,36;MFRL=394,329;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=58.34 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:3,14:0.789:17:2,4:1,7:2,1,10,4 +K03455 4526 . T C . . DP=13;ECNT=21;MBQ=0,36;MFRL=0,323;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=57.35 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,13:0.933:13:0,7:0,6:0,0,11,2 +K03455 4528 . T TCA . . DP=13;ECNT=21;MBQ=38,37;MFRL=323,421;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=5.52 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:11,2:0.200:13:5,1:4,1:0|1:4528_T_TCA:4528:10,1,1,1 +K03455 4530 . CTT C . . DP=13;ECNT=21;MBQ=35,38;MFRL=323,421;MMQ=60,60;MPOS=34;POPAF=7.30;RPA=4,2;RU=T;STR;TLOD=6.12 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:10,2:0.213:12:5,1:4,1:0|1:4528_T_TCA:4528:9,1,1,1 +K03455 4532 . T C . . DP=13;ECNT=21;MBQ=0,33;MFRL=0,323;MMQ=60,60;MPOS=21;POPAF=7.30;TLOD=39.42 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,11:0.926:11:0,7:0,2:0,0,11,0 diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-out2.vcf --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/Mutect2-out2.vcf Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,84 @@ +##fileformat=VCFv4.2 +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##GATKCommandLine= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##MutectVersion=2.2 +##contig= +##filtering_status=Warning: unfiltered Mutect 2 calls. Please run FilterMutectCalls to remove false positives. +##source=Mutect2 +##tumor_sample=SRR8525881 +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SRR8525881 +K03455 4861 . A G . . DP=1;ECNT=14;MBQ=0,37;MFRL=0,400;MMQ=60,60;MPOS=10;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,1:0.667:1:0,1:0,0:0,0,1,0 +K03455 4918 . G A . . DP=7;ECNT=14;MBQ=38,37;MFRL=303,370;MMQ=60,60;MPOS=19;POPAF=7.30;TLOD=8.65 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:3,3:0.499:6:2,0:1,2:2,1,0,3 +K03455 4923 . A G . . DP=8;ECNT=14;MBQ=0,33;MFRL=0,314;MMQ=60,60;MPOS=17;POPAF=7.30;TLOD=34.22 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,8:0.900:8:0,3:0,3:0,0,3,5 +K03455 4949 . G A . . DP=12;ECNT=14;MBQ=38,37;MFRL=285,264;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=27.49 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:5,7:0.573:12:2,2:1,4:0|1:4949_G_A:4949:2,3,2,5 +K03455 4952 . C T . . DP=13;ECNT=14;MBQ=36,37;MFRL=285,264;MMQ=60,60;MPOS=33;POPAF=7.30;TLOD=27.48 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:5,7:0.573:12:1,2:2,5:0|1:4949_G_A:4949:2,3,2,5 +K03455 4988 . T C . . DP=22;ECNT=14;MBQ=36,38;MFRL=285,278;MMQ=60,60;MPOS=37;POPAF=7.30;TLOD=14.99 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:17,5:0.250:22:5,2:8,2:6,11,3,2 +K03455 4991 . T C . . DP=22;ECNT=14;MBQ=37,28;MFRL=271,309;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=4.85 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:20,2:0.125:22:8,0:10,1:9,11,0,2 +K03455 4996 . AC GA . . DP=25;ECNT=14;MBQ=38,32;MFRL=285,203;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=5.72 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:21,3:0.152:24:6,1:10,1:8,13,1,2 +K03455 4997 . C T,A . . DP=25;ECNT=14;MBQ=38,37,38;MFRL=282,292,264;MMQ=60,60,60;MPOS=10,31;POPAF=7.30,7.30;TLOD=8.86,34.99 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:8,3,13:0.148,0.517:24:4,0,5:3,2,8:5,3,5,11 +K03455 5004 . G A . . DP=26;ECNT=14;MBQ=37,36;MFRL=262,288;MMQ=60,60;MPOS=51;POPAF=7.30;TLOD=4.21 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:24,2:0.107:26:7,1:13,1:8,16,1,1 +K03455 5015 . A G . . DP=34;ECNT=14;MBQ=38,38;MFRL=269,258;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=88.69 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:10,22:0.677:32:4,5:6,16:4,6,5,17 +K03455 5018 . A G . . DP=34;ECNT=14;MBQ=38,38;MFRL=262,261;MMQ=60,60;MPOS=26;POPAF=7.30;TLOD=11.32 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:30,4:0.139:34:9,0:15,4:10,20,0,4 +K03455 5027 . G A . . DP=37;ECNT=14;MBQ=37,38;MFRL=260,229;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=12.64 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:31,5:0.157:36:10,0:18,5:0|1:5027_G_A:5027:10,21,0,5 +K03455 5036 . G A . . DP=36;ECNT=14;MBQ=39,38;MFRL=256,229;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=12.71 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:30,5:0.162:35:9,0:18,4:0|1:5027_G_A:5027:10,20,0,5 +K03455 5076 . A G . . DP=37;ECNT=27;MBQ=38,37;MFRL=278,190;MMQ=60,60;MPOS=54;POPAF=7.30;TLOD=15.35 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:31,5:0.158:36:5,0:25,5:0|1:5076_A_G:5076:5,26,0,5 +K03455 5098 . A G . . DP=42;ECNT=27;MBQ=38,38;MFRL=190,292;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=126.26 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:7,32:0.804:39:0,4:7,22:0,7,4,28 +K03455 5099 . C T . . DP=42;ECNT=27;MBQ=38,37;MFRL=292,190;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=22.52 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:34,7:0.186:41:4,0:28,6:0|1:5076_A_G:5076:4,30,0,7 +K03455 5121 . C T . . DP=41;ECNT=27;MBQ=37,38;MFRL=257,205;MMQ=60,60;MPOS=26;POPAF=7.30;TLOD=24.97 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:33,8:0.209:41:3,1:28,6:3,30,1,7 +K03455 5130 . TG CA . . DP=38;ECNT=27;MBQ=38,38;MFRL=194,275;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=122.82 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:8,30:0.778:38:0,0:7,28:0,8,2,28 +K03455 5131 . G A . . DP=38;ECNT=27;MBQ=0,38;MFRL=0,205;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=23.77 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,8:0.932:8:0,1:0,6:0,0,1,7 +K03455 5133 . T C . . DP=36;ECNT=27;MBQ=38,36;MFRL=293,216;MMQ=60,60;MPOS=33;POPAF=7.30;TLOD=31.26 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:27,9:0.268:36:2,0:23,8:0|1:5133_T_C:5133:2,25,0,9 +K03455 5137 . GG AG,AA . . DP=35;ECNT=27;MBQ=39,39,39;MFRL=194,216,337;MMQ=60,60,60;MPOS=34,28;POPAF=7.30,7.30;TLOD=31.44,66.06 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:8,9,18:0.270,0.495:35:0,0,1:7,8,16:0,8,1,26 +K03455 5146 . AGG A . . DP=33;ECNT=27;MBQ=38,38;MFRL=315,216;MMQ=60,60;MPOS=43;POPAF=7.30;RPA=4,2;RU=G;STR;TLOD=31.38 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:24,9:0.286:33:1,0:22,9:0|1:5133_T_C:5133:1,23,0,9 +K03455 5147 . G A . . DP=33;ECNT=27;MBQ=38,37;MFRL=181,343;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=66.04 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:7,17:0.694:24:0,0:6,13:0|1:5147_G_A:5147:0,7,1,16 +K03455 5153 . GGTTT G . . DP=29;ECNT=27;MBQ=38,38;MFRL=296,216;MMQ=60,60;MPOS=50;POPAF=7.30;TLOD=32.02 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:20,9:0.323:29:0,0:19,9:0|1:5133_T_C:5133:0,20,0,9 +K03455 5155 . T C . . DP=28;ECNT=27;MBQ=38,38;MFRL=169,331;MMQ=60,60;MPOS=55;POPAF=7.30;TLOD=49.64 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:6,13:0.669:19:0,0:6,12:0|1:5147_G_A:5147:0,6,0,13 +K03455 5156 . T A . . DP=28;ECNT=27;MBQ=38,37;MFRL=315,181;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=16.29 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:14,5:0.283:19:0,0:14,5:0|1:5156_T_A:5156:0,14,0,5 +K03455 5157 . T G . . DP=28;ECNT=27;MBQ=38,37;MFRL=181,331;MMQ=60,60;MPOS=54;POPAF=7.30;TLOD=49.65 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:5,13:0.703:18:0,0:5,13:0,5,0,13 +K03455 5169 . C T . . DP=20;ECNT=27;MBQ=39,39;MFRL=296,170;MMQ=60,60;MPOS=37;POPAF=7.30;TLOD=20.02 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:14,6:0.318:20:0,0:14,6:0,14,0,6 +K03455 5175 . A T . . DP=19;ECNT=27;MBQ=37,39;MFRL=203,299;MMQ=60,60;MPOS=55;POPAF=7.30;TLOD=34.23 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:10,9:0.476:19:0,0:9,9:0|1:5147_G_A:5147:0,10,0,9 +K03455 5177 . G C . . DP=18;ECNT=27;MBQ=39,37;MFRL=299,216;MMQ=60,60;MPOS=62;POPAF=7.30;TLOD=25.72 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:11,7:0.400:18:0,0:10,7:0|1:5133_T_C:5133:0,11,0,7 +K03455 5179 . CC AC,AA . . DP=17;ECNT=27;MBQ=39,36,39;MFRL=366,216,315;MMQ=60,60,60;MPOS=60,51;POPAF=7.30,7.30;TLOD=26.12,30.50 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:2,7,8:0.400,0.450:17:0,0,0:2,7,8:0,2,0,15 +K03455 5189 . G A . . DP=18;ECNT=27;MBQ=39,39;MFRL=203,315;MMQ=60,60;MPOS=41;POPAF=7.30;TLOD=30.08 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:10,8:0.450:18:1,0:9,8:1,9,0,8 +K03455 5190 . A G . . DP=18;ECNT=27;MBQ=39,20;MFRL=293,181;MMQ=60,60;MPOS=62;POPAF=7.30;TLOD=9.31 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:15,3:0.200:18:0,0:15,2:0|1:5156_T_A:5156:0,15,1,2 +K03455 5196 . T C . . DP=18;ECNT=27;MBQ=39,37;MFRL=293,181;MMQ=60,60;MPOS=56;POPAF=7.30;TLOD=9.19 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:15,3:0.200:18:0,1:15,2:0,15,1,2 +K03455 5220 . G A . . DP=16;ECNT=27;MBQ=38,38;MFRL=312,219;MMQ=60,60;MPOS=22;POPAF=7.30;TLOD=21.54 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:10,6:0.389:16:1,0:9,6:0|1:5220_G_A:5220:1,9,0,6 +K03455 5223 . T A . . DP=16;ECNT=27;MBQ=37,39;MFRL=293,181;MMQ=60,60;MPOS=29;POPAF=7.30;TLOD=9.40 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:13,3:0.222:16:0,1:13,2:0|1:5223_T_A:5223:0,13,1,2 +K03455 5230 . T C . . DP=16;ECNT=27;MBQ=38,39;MFRL=293,181;MMQ=60,60;MPOS=22;POPAF=7.30;TLOD=9.40 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:13,3:0.222:16:0,1:12,2:0|1:5223_T_A:5223:0,13,1,2 +K03455 5233 . G A . . DP=12;ECNT=27;MBQ=38,38;MFRL=293,216;MMQ=60,60;MPOS=11;POPAF=7.30;TLOD=18.49 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:7,5:0.429:12:0,0:6,5:0|1:5220_G_A:5220:1,6,0,5 +K03455 5236 . A G . . DP=12;ECNT=27;MBQ=37,38;MFRL=216,256;MMQ=60,60;MPOS=14;POPAF=7.30;TLOD=22.57 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:5,6:0.539:11:0,1:5,5:0,5,1,5 +K03455 5240 . C T . . DP=11;ECNT=27;MBQ=37,37;MFRL=219,162;MMQ=60,60;MPOS=-1;POPAF=7.30;TLOD=9.66 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:8,3:0.308:11:0,0:7,3:1,7,0,3 diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-out3.vcf --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/Mutect2-out3.vcf Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,66 @@ +##fileformat=VCFv4.2 +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##GATKCommandLine= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##MutectVersion=2.2 +##contig= +##filtering_status=Warning: unfiltered Mutect 2 calls. Please run FilterMutectCalls to remove false positives. +##source=Mutect2 +##tumor_sample=SRR8525881 +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SRR8525881 +K03455 2652 . G A . . BQHIST=16,1,0,18,9,0,20,25,0,29,5,0,32,20,0,33,12,0,34,2,0,37,27,0,38,13,0,39,13,0;DP=129;ECNT=6;MBQ=0,33;MFRL=0,273;MMQ=60,60;MPOS=5;POPAF=7.30;TLOD=571.21 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,129:0.992:129:0,57:0,60:0|1:2652_G_A:2652:0,0,115,14 +K03455 2660 . T C . . BQHIST=15,1,0,16,1,0,17,2,0,20,24,0,29,1,0,32,4,0,33,3,0,34,2,0,35,6,0,37,48,0,38,27,0,39,20,0;DP=162;ECNT=6;MBQ=0,37;MFRL=0,274;MMQ=60,60;MPOS=13;POPAF=7.30;TLOD=717.35 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,162:0.993:162:0,66:0,69:0|1:2652_G_A:2652:0,0,145,17 +K03455 2664 . A G . . BQHIST=18,0,2,19,0,2,20,0,27,30,0,3,32,0,15,33,0,13,34,0,1,35,0,4,36,0,1,37,0,17,38,0,65,39,0,13;DP=165;ECNT=6;MBQ=0,37;MFRL=0,273;MMQ=60,60;MPOS=14;POPAF=7.30;TLOD=572.42 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,165:0.993:165:0,80:0,79:0,0,148,17 +K03455 2669 . G A . . BQHIST=16,1,0,18,1,0,19,2,0,20,32,0,29,1,0,32,5,0,33,8,0,34,3,0,36,1,0,37,45,0,38,44,0,39,31,0;DP=177;ECNT=6;MBQ=0,37;MFRL=0,274;MMQ=60,60;MPOS=17;POPAF=7.30;TLOD=755.51 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,174:0.994:174:0,84:0,86:0|1:2652_G_A:2652:0,0,155,19 +K03455 2677 . A G . . BQHIST=15,3,0,17,1,0,18,1,0,19,4,0,20,35,2,33,6,0,34,9,0,36,2,0,37,29,0,38,36,0,39,55,2;DP=186;ECNT=6;MBQ=38,30;MFRL=273,240;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=5.37 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:181,4:0.024:185:83,2:89,2:162,19,3,1 +K03455 2720 . T C . . BQHIST=14,3,0,16,10,0,17,1,0,18,20,0,20,65,0,30,1,0,32,2,0,33,25,0,34,9,0,36,12,0,37,81,0,38,24,0,39,40,0;DP=339;ECNT=6;MBQ=0,36;MFRL=0,263;MMQ=60,60;MPOS=51;POPAF=7.30;TLOD=1040.98 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,295:0.996:295:0,120:0,139:0,0,250,45 +K03455 2797 . G A . . BQHIST=15,2,0,16,3,0,17,1,0,18,22,0,20,188,0,32,5,0,33,21,0,35,8,0,36,8,0,37,56,0,38,62,0,39,110,0;DP=521;ECNT=12;MBQ=0,35;MFRL=0,246;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=1710.50 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,497:0.998:497:0,245:0,213:0,0,334,163 +K03455 2831 . A T . . BQHIST=14,5,0,15,12,0,16,2,1,17,48,3,18,12,10,19,3,0,20,138,0,29,2,0,30,5,1,31,6,0,32,9,0,33,40,2,34,14,0,35,1,0,36,6,0,37,52,0,38,43,0,39,102,0;DP=534;ECNT=12;MBQ=33,18;MFRL=240,262;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=4.34 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:502,17:0.023:519:221,1:197,2:298,204,0,17 +K03455 2848 . T TA . . BQHIST=14,0,2,15,0,18,16,0,11,17,0,1,20,6,241,27,0,1,29,0,1,30,1,14,31,0,1,32,0,13,33,2,22,34,0,11,35,0,1,36,0,20,37,0,47,38,1,71,39,3,81;DP=596;ECNT=12;MBQ=30,30;MFRL=257,246;MMQ=60,60;MPOS=32;POPAF=7.30;RPA=6,7;RU=A;STR;TLOD=1256.09 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:13,560:0.982:573:6,264:7,260:8,5,299,261 +K03455 2849 . A AG . . BQHIST=14,0,8,15,0,1,16,0,2,17,0,1,18,0,16,19,1,0,20,2,269,27,0,1,29,0,2,30,1,8,31,0,2,32,0,4,33,0,17,34,1,6,35,0,4,36,0,33,37,1,38,38,0,100,39,2,62;DP=594;ECNT=12;MBQ=20,32;MFRL=245,276;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=3.21 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:577,8:0.012:585:285,2:261,5:309,268,4,4 +K03455 2872 . T A . . BQHIST=14,1,2,15,2,0,16,0,10,17,6,6,18,15,51,19,0,38,20,0,164,27,1,1,29,0,16,30,0,3,31,0,9,32,0,20,33,0,29,34,0,12,35,0,12,36,0,9,37,0,78,38,0,46,39,0,53;DP=620;ECNT=12;MBQ=29,18;MFRL=240,283;MMQ=60,60;MPOS=29;POPAF=7.30;TLOD=3.49 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:559,25:0.023:584:222,1:230,0:259,300,25,0 +K03455 2874 . C T . . BQHIST=16,0,6,17,1,5,18,0,53,19,0,13,20,0,199,29,0,4,30,0,2,31,0,11,32,0,31,33,0,40,34,0,13,35,0,12,36,0,11,37,0,85,38,0,53,39,0,50;DP=598;ECNT=12;MBQ=17,32;MFRL=360,241;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=2009.02 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:1,595:0.997:596:0,262:0,249:1,0,292,303 +K03455 2882 . G T . . BQHIST=14,1,0,16,5,0,17,7,0,18,21,0,19,12,0,20,194,0,29,2,0,30,4,0,31,4,1,32,3,0,33,25,0,34,1,0,35,19,0,36,11,0,37,82,0,38,50,0,39,111,0;DP=569;ECNT=12;MBQ=31,33;MFRL=360,246;MMQ=60,60;MPOS=40;POPAF=7.30;TLOD=1892.82 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:1,568:0.997:569:1,254:0,252:1,0,287,281 +K03455 2889 . G A . . BQHIST=14,0,11,15,0,1,16,0,4,17,0,4,18,0,13,19,0,17,20,0,189,29,0,1,30,0,3,31,0,1,32,0,7,33,2,31,34,1,4,35,0,9,36,0,14,37,1,76,38,1,70,39,2,94,40,0,1;DP=568;ECNT=12;MBQ=33,37;MFRL=249,308;MMQ=60,60;MPOS=53;POPAF=7.30;TLOD=8.92 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:552,7:0.016:559:244,4:256,3:282,270,6,1 +K03455 2891 . A G . . BQHIST=14,0,1,16,4,0,17,9,0,18,18,0,19,8,0,20,176,6,30,1,0,31,4,0,32,7,0,33,25,1,34,2,0,35,26,0,36,7,1,37,75,0,38,84,0,39,87,0;DP=564;ECNT=12;MBQ=35,20;MFRL=249,231;MMQ=60,60;MPOS=45;POPAF=7.30;TLOD=8.84 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:543,9:0.013:552:237,4:257,4:276,267,5,4 +K03455 2894 . T C . . BQHIST=14,1,0,15,5,0,16,4,0,17,13,0,18,32,0,19,4,0,20,160,0,27,1,0,29,3,0,30,5,0,32,5,0,33,24,0,34,12,0,35,5,0,36,8,0,37,81,0,38,68,0,39,113,0;DP=551;ECNT=12;MBQ=0,35;MFRL=0,250;MMQ=60,60;MPOS=41;POPAF=7.30;TLOD=1769.47 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,551:0.998:551:0,236:0,249:0,0,280,271 +K03455 2906 . C T . . BQHIST=16,0,2,18,0,4,19,0,10,20,0,144,30,0,2,31,0,1,32,0,3,33,0,19,34,0,3,35,0,19,36,0,11,37,0,57,38,0,75,39,0,145;DP=495;ECNT=12;MBQ=0,37;MFRL=0,250;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=2348.23 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,495:0.998:495:0,225:0,254:0|1:2906_C_T:2906:0,0,235,260 +K03455 2913 . G A . . BQHIST=18,4,0,19,5,0,20,132,0,29,1,0,30,2,0,31,2,0,32,2,0,33,17,0,34,1,0,35,4,0,36,1,0,37,62,0,38,65,0,39,182,0,40,4,0;DP=492;ECNT=12;MBQ=0,38;MFRL=0,251;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=2341.59 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,492:0.998:492:0,224:0,251:0|1:2906_C_T:2906:0,0,234,258 +K03455 2987 . C T . . BQHIST=16,0,3,17,0,3,19,0,11,20,0,15,30,0,2,31,0,9,32,0,7,33,0,31,34,0,8,35,0,9,36,0,1,37,0,47,38,0,34,39,0,39;DP=223;ECNT=4;MBQ=0,37;MFRL=0,264;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=847.14 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,219:0.995:219:0,99:0,103:0,0,86,133 +K03455 2992 . T G . . BQHIST=16,1,0,17,3,0,18,1,7,19,23,0,20,13,1,29,1,0,31,8,0,32,8,0,33,18,1,34,9,0,35,4,0,36,4,0,37,35,0,38,30,0,39,21,0;DP=206;ECNT=4;MBQ=36,18;MFRL=271,260;MMQ=60,60;MPOS=29;POPAF=7.30;TLOD=3.86 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:179,9:0.043:188:74,2:77,0:74,105,9,0 +K03455 3016 . C A . . BQHIST=14,0,2,15,0,2,18,6,26,29,0,1,30,0,3,32,0,5,33,1,12,34,0,2,35,0,2,36,0,8,37,0,5,38,0,16,39,0,4;DP=100;ECNT=4;MBQ=33,18;MFRL=276,255;MMQ=60,60;MPOS=5;POPAF=7.30;TLOD=5.62 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:88,7:0.081:95:24,1:34,0:61,27,7,0 +K03455 3020 . A G . . BQHIST=14,0,12,16,0,1,18,0,1,27,0,3,29,0,3,30,0,8,32,0,8,33,0,2,34,0,1,35,0,1,36,0,21,37,0,4,38,0,23,39,0,1;DP=95;ECNT=4;MBQ=0,36;MFRL=0,284;MMQ=60,60;MPOS=7;POPAF=7.30;TLOD=324.41 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,90:0.989:90:0,32:0,43:0,0,65,25 diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-out4.vcf --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/Mutect2-out4.vcf Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,75 @@ +##fileformat=VCFv4.2 +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##GATKCommandLine= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##MutectVersion=2.2 +##contig= +##filtering_status=Warning: unfiltered Mutect 2 calls. Please run FilterMutectCalls to remove false positives. +##source=Mutect2 +##tumor_sample=SRR8525881 +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SRR8525881 +K03455 6822 . GT AC . . DP=2;ECNT=1;MBQ=0,29;MFRL=0,117;MMQ=60,60;MPOS=79;POPAF=7.30;TLOD=8.52 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,2:0.750:2:0,0:0,2:0,0,0,2 +K03455 6902 . A C . . DP=1;ECNT=8;MBQ=0,39;MFRL=0,162;MMQ=60,60;MPOS=65;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:6902_A_C:6902:0,0,0,1 +K03455 6905 . A G . . DP=1;ECNT=8;MBQ=0,39;MFRL=0,162;MMQ=60,60;MPOS=62;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:6902_A_C:6902:0,0,0,1 +K03455 6911 . T C . . DP=1;ECNT=8;MBQ=0,39;MFRL=0,162;MMQ=60,60;MPOS=56;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:6902_A_C:6902:0,0,0,1 +K03455 6917 . G A . . DP=1;ECNT=8;MBQ=0,39;MFRL=0,162;MMQ=60,60;MPOS=50;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:6902_A_C:6902:0,0,0,1 +K03455 6920 . G A . . DP=1;ECNT=8;MBQ=0,39;MFRL=0,162;MMQ=60,60;MPOS=47;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:6902_A_C:6902:0,0,0,1 +K03455 6923 . C T . . DP=1;ECNT=8;MBQ=0,39;MFRL=0,162;MMQ=60,60;MPOS=44;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:6902_A_C:6902:0,0,0,1 +K03455 6931 . C A . . DP=1;ECNT=8;MBQ=0,39;MFRL=0,162;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:6902_A_C:6902:0,0,0,1 +K03455 6936 . C G . . DP=1;ECNT=8;MBQ=0,39;MFRL=0,162;MMQ=60,60;MPOS=31;POPAF=7.30;TLOD=4.20 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:6902_A_C:6902:0,0,0,1 +K03455 7441 . A ACCT . . DP=1;ECNT=23;MBQ=0,39;MFRL=0,486;MMQ=60,60;MPOS=-2147483648;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:7441_A_ACCT:7441:0,0,0,1 +K03455 7443 . A AGTG . . DP=1;ECNT=23;MBQ=0,39;MFRL=0,486;MMQ=60,60;MPOS=94;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:7441_A_ACCT:7441:0,0,0,1 +K03455 7449 . G A . . DP=1;ECNT=23;MBQ=0,39;MFRL=0,486;MMQ=60,60;MPOS=100;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:7441_A_ACCT:7441:0,0,0,1 +K03455 7452 . GGA G . . DP=1;ECNT=23;MBQ=0,39;MFRL=0,486;MMQ=60,60;MPOS=103;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:7441_A_ACCT:7441:0,0,0,1 +K03455 7455 . A ATG . . DP=1;ECNT=23;MBQ=0,39;MFRL=0,486;MMQ=60,60;MPOS=106;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:7441_A_ACCT:7441:0,0,0,1 +K03455 7457 . TGA T . . DP=1;ECNT=23;MBQ=0,39;MFRL=0,486;MMQ=60,60;MPOS=108;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:7441_A_ACCT:7441:0,0,0,1 +K03455 7460 . CA C . . DP=1;ECNT=23;MBQ=0,37;MFRL=0,486;MMQ=60,60;MPOS=111;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,1:0.667:1:0,0:0,1:0|1:7441_A_ACCT:7441:0,0,0,1 +K03455 7468 . CC TA . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=114;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,1:0|1:7441_A_ACCT:7441:0,0,0,2 +K03455 7478 . C T . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=111;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7441_A_ACCT:7441:0,0,0,2 +K03455 7491 . A T . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=104;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7441_A_ACCT:7441:0,0,0,2 +K03455 7494 . A G . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=101;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7441_A_ACCT:7441:0,0,0,2 +K03455 7508 . GA AG . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=87;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7441_A_ACCT:7441:0,0,0,2 +K03455 7541 . C CGA . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=54;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7542 . AGT A . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=53;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7548 . C A . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=47;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7555 . GA AT . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=40;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7560 . TC GT . . DP=2;ECNT=23;MBQ=0,38;MFRL=0,586;MMQ=60,50;MPOS=35;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7568 . T C . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=-1073741804;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7571 . T C . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=24;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7581 . C A . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=14;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7584 . T C . . DP=2;ECNT=23;MBQ=0,39;MFRL=0,586;MMQ=60,50;MPOS=11;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7603 . A AGGG . . DP=2;ECNT=23;MBQ=0,37;MFRL=0,586;MMQ=60,50;MPOS=-8;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,2:0|1:7541_C_CGA:7541:0,0,0,2 +K03455 7604 . T G . . DP=2;ECNT=23;MBQ=0,33;MFRL=0,586;MMQ=60,50;MPOS=-22;POPAF=7.30;TLOD=8.70 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:0,2:0.667:2:0,0:0,1:0|1:7541_C_CGA:7541:0,0,0,2 diff -r 000000000000 -r f41a1e03538b test-data/Mutect2-out5-1.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/Mutect2-out5-1.tabular Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,9721 @@ +#track graphType=line +Chromosome Start End Feature ActivityProfile +K03455 0 1 state 0.00000 +K03455 1 2 state 0.00000 +K03455 2 3 state 0.00000 +K03455 3 4 state 0.00000 +K03455 4 5 state 0.00000 +K03455 5 6 state 0.00000 +K03455 6 7 state 0.00000 +K03455 7 8 state 0.00000 +K03455 8 9 state 0.00000 +K03455 9 10 state 0.00000 +K03455 10 11 state 0.00000 +K03455 11 12 state 0.00000 +K03455 12 13 state 0.00000 +K03455 13 14 state 0.00000 +K03455 14 15 state 0.00000 +K03455 15 16 state 0.00000 +K03455 16 17 state 0.00000 +K03455 17 18 state 0.00000 +K03455 18 19 state 0.00000 +K03455 19 20 state 0.00000 +K03455 20 21 state 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Please run FilterMutectCalls to remove false positives. +##source=Mutect2 +##tumor_sample=SRR8525881 +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT SRR8525881 +K03455 2097 . G A . . DP=14;ECNT=41;MBQ=0,20;MFRL=0,160;MMQ=60,60;MPOS=34;POPAF=7.30;TLOD=75.39 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,14:0.929:14:0,7:0,5:0,0,9,5 +K03455 2106 . T C . . DP=16;ECNT=41;MBQ=20,30;MFRL=160,90;MMQ=60,60;MPOS=24;POPAF=7.30;TLOD=12.26 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:12,4:0.266:16:6,1:6,1:8,4,2,2 +K03455 2110 . TA CA,CT . . DP=17;ECNT=41;MBQ=0,20,35;MFRL=0,159,161;MMQ=60,60,60;MPOS=42,2;POPAF=7.30,7.30;TLOD=55.54,10.69 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:0,13,4:0.754,0.187:17:0,6,1:0,7,3:0,0,11,6 +K03455 2118 . A G . . DP=17;ECNT=41;MBQ=32,20;MFRL=90,197;MMQ=60,60;MPOS=55;POPAF=7.30;TLOD=30.61 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:5,10:0.715:15:1,4:4,5:3,2,7,3 +K03455 2130 . T C . . DP=17;ECNT=41;MBQ=20,37;MFRL=159,161;MMQ=60,60;MPOS=22;POPAF=7.30;TLOD=10.45 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:13,4:0.199:17:4,1:8,3:8,5,3,1 +K03455 2136 . T C . . DP=17;ECNT=41;MBQ=20,38;MFRL=159,161;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=10.45 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:13,4:0.199:17:4,1:9,2:8,5,3,1 +K03455 2155 . A T . . DP=19;ECNT=41;MBQ=39,20;MFRL=90,234;MMQ=60,60;MPOS=56;POPAF=7.30;TLOD=39.43 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:5,14:0.777:19:1,5:4,6:3,2,10,4 +K03455 2169 . A G . . DP=22;ECNT=41;MBQ=0,35;MFRL=0,233;MMQ=60,60;MPOS=32;POPAF=7.30;TLOD=100.81 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,22:0.955:22:0,9:0,12:0,0,16,6 +K03455 2183 . C T . . DP=27;ECNT=41;MBQ=0,33;MFRL=0,233;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=129.24 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,27:0.962:27:0,9:0,13:0,0,18,9 +K03455 2189 . TA AA,AG . . DP=29;ECNT=41;MBQ=0,36,20;MFRL=0,232,265;MMQ=60,60,60;MPOS=25,22;POPAF=7.30,7.30;TLOD=46.72,54.99 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:0,13,14:0.424,0.539:27:0,6,5:0,6,5:0,0,18,9 +K03455 2195 . C G,T . . DP=29;ECNT=41;MBQ=37,30,35;MFRL=232,265,245;MMQ=60,60,60;MPOS=19,23;POPAF=7.30,7.30;TLOD=41.44,16.79 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:9,14,6:0.500,0.250:29:3,8,3:5,6,3:5,4,14,6 +K03455 2197 . A G . . DP=29;ECNT=41;MBQ=31,37;MFRL=257,232;MMQ=60,60;MPOS=10;POPAF=7.30;TLOD=29.34 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:20,9:0.259:29:9,3:7,5:14,6,5,4 +K03455 2200 . ACTC A . . DP=30;ECNT=41;MBQ=31,36;MFRL=265,232;MMQ=60,60;MPOS=17;POPAF=7.30;TLOD=13.22 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:23,5:0.153:28:10,3:6,2:0|1:2200_ACTC_A:2200:15,8,3,2 +K03455 2202 . T C . . DP=30;ECNT=41;MBQ=35,35;MFRL=265,182;MMQ=60,60;MPOS=12;POPAF=7.30;TLOD=5.58 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:21,4:0.166:25:9,1:7,1:0|1:2202_T_C:2202:14,7,2,2 +K03455 2205 . C A . . DP=30;ECNT=41;MBQ=36,26;MFRL=254,286;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=5.06 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:28,2:0.107:30:13,1:10,0:0|1:2202_T_C:2202:19,9,1,1 +K03455 2213 . A G . . DP=31;ECNT=41;MBQ=38,37;MFRL=232,265;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=102.83 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:7,24:0.793:31:4,11:2,8:4,3,17,7 +K03455 2214 . G A . . DP=28;ECNT=41;MBQ=33,26;MFRL=254,286;MMQ=60,60;MPOS=24;POPAF=7.30;TLOD=5.06 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:26,2:0.107:28:12,1:10,0:0|1:2214_G_A:2214:17,9,1,1 +K03455 2221 . C A . . DP=35;ECNT=41;MBQ=34,35;MFRL=251,232;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=12.67 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:30,5:0.114:35:16,1:11,2:0|1:2200_ACTC_A:2200:22,8,3,2 +K03455 2223 . G A . . DP=35;ECNT=41;MBQ=37,34;MFRL=251,211;MMQ=60,60;MPOS=33;POPAF=7.30;TLOD=47.57 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:20,14:0.446:34:12,7:7,6:17,3,7,7 +K03455 2224 . AT GG,CT . . DP=34;ECNT=41;MBQ=34,33,38;MFRL=265,212,253;MMQ=60,60,60;MPOS=37,18;POPAF=7.30,7.30;TLOD=13.11,33.64 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:19,5,10:0.118,0.324:34:9,3,7:8,2,3:11,8,13,2 +K03455 2225 . T G . . DP=34;ECNT=41;MBQ=37,38;MFRL=253,286;MMQ=60,60;MPOS=35;POPAF=7.30;TLOD=5.10 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:27,2:0.099:29:16,1:11,1:0|1:2214_G_A:2214:20,7,1,1 +K03455 2226 . A G . . DP=34;ECNT=41;MBQ=20,37;MFRL=232,253;MMQ=60,60;MPOS=20;POPAF=7.30;TLOD=114.56 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:7,27:0.818:34:4,16:2,9:0|1:2226_A_G:2226:4,3,20,7 +K03455 2230 . A C . . DP=36;ECNT=41;MBQ=28,37;MFRL=232,253;MMQ=60,60;MPOS=24;POPAF=7.30;TLOD=118.22 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:8,28:0.800:36:3,15:3,11:0|1:2226_A_G:2226:4,4,21,7 +K03455 2234 . A G . . DP=42;ECNT=41;MBQ=29,37;MFRL=232,245;MMQ=60,60;MPOS=15;POPAF=7.30;TLOD=144.85 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:8,34:0.821:42:2,20:3,11:0|1:2226_A_G:2226:4,4,24,10 +K03455 2235 . AC GA . . DP=42;ECNT=41;MBQ=36,34;MFRL=248,212;MMQ=60,60;MPOS=48;POPAF=7.30;TLOD=12.53 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:37,5:0.103:42:22,2:11,2:0|1:2200_ACTC_A:2200:25,12,3,2 +K03455 2236 . CTG C,GTG . . DP=42;ECNT=41;MBQ=38,35,38;MFRL=251,240,286;MMQ=60,60,60;MPOS=16,46;POPAF=7.30,7.30;TLOD=61.99,5.13 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:14,20,2:0.528,0.082:36:8,12,1:5,8,1:12,2,13,9 +K03455 2239 . TA CC . . DP=45;ECNT=41;MBQ=36,38;MFRL=232,251;MMQ=60,60;MPOS=30;POPAF=7.30;TLOD=48.80 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:29,14:0.350:43:15,9:10,4:17,12,12,2 +K03455 2240 . A ACC . . DP=45;ECNT=41;MBQ=37,34;MFRL=232,211;MMQ=60,60;MPOS=-2147483648;POPAF=7.30;TLOD=61.64 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:9,24:0.731:33:4,13:4,9:5,4,14,10 +K03455 2248 . A G . . DP=46;ECNT=41;MBQ=20,38;MFRL=197,247;MMQ=60,60;MPOS=28;POPAF=7.30;TLOD=185.50 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 1|0:6,40:0.884:46:3,21:3,16:1|0:2200_ACTC_A:2200:4,2,27,13 +K03455 2250 . T C . . DP=46;ECNT=41;MBQ=38,37;MFRL=247,197;MMQ=60,60;MPOS=42;POPAF=7.30;TLOD=15.87 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:40,6:0.116:46:23,3:16,3:0|1:2200_ACTC_A:2200:27,13,4,2 +K03455 2258 . GG AA . . DP=48;ECNT=41;MBQ=38,39;MFRL=241,235;MMQ=60,60;MPOS=37;POPAF=7.30;TLOD=163.72 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:7,41:0.843:48:3,23:3,17:0|1:2258_GG_AA:2258:6,1,27,14 +K03455 2259 . G A . . DP=48;ECNT=41;MBQ=0,37;MFRL=0,242;MMQ=60,60;MPOS=37;POPAF=7.30;TLOD=17.05 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 1|0:0,14:0.919:14:0,5:0,8:1|0:2258_GG_AA:2258:0,0,10,4 +K03455 2282 . C T . . DP=62;ECNT=41;MBQ=35,20;MFRL=241,182;MMQ=60,60;MPOS=54;POPAF=7.30;TLOD=12.25 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:57,5:0.075:62:24,2:24,2:32,25,3,2 +K03455 2285 . C T . . DP=65;ECNT=41;MBQ=28,38;MFRL=239,209;MMQ=60,60;MPOS=50;POPAF=7.30;TLOD=30.08 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:54,10:0.164:64:24,4:21,4:30,24,7,3 +K03455 2287 . CA AT . . DP=68;ECNT=41;MBQ=33,37;MFRL=232,285;MMQ=60,60;MPOS=41;POPAF=7.30;TLOD=17.29 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:61,7:0.128:68:26,1:25,2:33,28,4,3 +K03455 2300 . G A . . DP=67;ECNT=41;MBQ=31,32;MFRL=219,236;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=209.86 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:16,49:0.754:65:5,20:7,22:8,8,26,23 +K03455 2303 . GCA G . . DP=65;ECNT=41;MBQ=36,31;MFRL=221,241;MMQ=60,60;MPOS=38;POPAF=7.30;TLOD=170.03 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:19,45:0.695:64:4,18:11,20:9,10,25,20 +K03455 2304 . CAA GTG . . DP=65;ECNT=41;MBQ=33,20;MFRL=219,235;MMQ=60,60;MPOS=36;POPAF=7.30;TLOD=3.43 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:16,19:0.471:35:6,4:9,12:8,8,10,9 +K03455 2306 . A ATG,G . . DP=64;ECNT=41;MBQ=20,32,33;MFRL=188,239,274;MMQ=60,60,60;MPOS=39,19;POPAF=7.30,7.30;TLOD=174.74,25.23 GT:AD:AF:DP:F1R2:F2R1:SB 0/1/2:7,48,9:0.738,0.173:64:4,20,2:3,23,5:4,3,30,27 +K03455 2315 . A G . . DP=63;ECNT=41;MBQ=37,38;MFRL=219,244;MMQ=60,60;MPOS=50;POPAF=7.30;TLOD=74.04 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:42,20:0.358:62:18,6:17,14:0|1:2315_A_G:2315:21,21,11,9 +K03455 2321 . A C . . DP=60;ECNT=41;MBQ=33,37;MFRL=212,244;MMQ=60,60;MPOS=45;POPAF=7.30;TLOD=74.63 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:39,20:0.377:59:18,6:18,14:0|1:2315_A_G:2315:19,20,11,9 +K03455 2349 . T C . . DP=53;ECNT=9;MBQ=34,35;MFRL=234,259;MMQ=60,60;MPOS=54;POPAF=7.30;TLOD=21.63 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:46,7:0.160:53:18,3:22,4:24,22,3,4 +K03455 2360 . G A . . DP=48;ECNT=9;MBQ=37,34;MFRL=235,232;MMQ=60,60;MPOS=43;POPAF=7.30;TLOD=20.61 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:41,7:0.175:48:16,3:22,3:22,19,3,4 +K03455 2362 . G A . . DP=48;ECNT=9;MBQ=0,37;MFRL=0,234;MMQ=60,60;MPOS=23;POPAF=7.30;TLOD=178.56 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:0,46:0.978:46:0,20:0,24:0,0,23,23 +K03455 2372 . A G . . DP=43;ECNT=9;MBQ=37,36;MFRL=237,227;MMQ=60,60;MPOS=31;POPAF=7.30;TLOD=22.64 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:36,7:0.192:43:8,2:17,4:19,17,3,4 +K03455 2374 . G A . . DP=34;ECNT=9;MBQ=37,37;MFRL=227,255;MMQ=60,60;MPOS=9;POPAF=7.30;TLOD=4.41 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:31,3:0.102:34:8,0:18,2:11,20,2,1 +K03455 2423 . A G . . DP=14;ECNT=9;MBQ=34,36;MFRL=248,221;MMQ=60,60;MPOS=10;POPAF=7.30;TLOD=5.14 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:12,2:0.187:14:3,0:8,2:0,12,0,2 +K03455 2436 . A G . . DP=4;ECNT=9;MBQ=16,33;MFRL=255,255;MMQ=60,60;MPOS=4;POPAF=7.30;TLOD=12.21 GT:AD:AF:DP:F1R2:F2R1:SB 0/1:1,3:0.671:4:0,0:0,3:0,1,0,3 +K03455 2438 . A G . . DP=4;ECNT=9;MBQ=24,33;MFRL=255,255;MMQ=60,60;MPOS=3;POPAF=7.30;TLOD=7.53 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:2,2:0.502:4:0,0:1,2:0|1:2438_A_G:2438:0,2,0,2 +K03455 2440 . T C . . DP=4;ECNT=9;MBQ=0,31;MFRL=255,255;MMQ=60,60;MPOS=1;POPAF=7.30;TLOD=7.53 GT:AD:AF:DP:F1R2:F2R1:PGT:PID:PS:SB 0|1:2,2:0.502:4:0,0:0,2:0|1:2438_A_G:2438:0,2,0,2 diff -r 000000000000 -r f41a1e03538b test-data/reference.fa --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reference.fa Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,2 @@ +>K03455 +TGGAAGGGCTAATTCACTCCCAACGAAGACAAGATATCCTTGATCTGTGGATCTACCACACACAAGGCTACTTCCCTGATTAGCAGAACTACACACCAGGGCCAGGGATCAGATATCCACTGACCTTTGGATGGTGCTACAAGCTAGTACCAGTTGAGCCAGAGAAGTTAGAAGAAGCCAACAAAGGAGAGAACACCAGCTTGTTACACCCTGTGAGCCTGCATGGAATGGATGACCCGGAGAGAGAAGTGTTAGAGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACATGGCCCGAGAGCTGCATCCGGAGTACTTCAAGAACTGCTGACATCGAGCTTGCTACAAGGGACTTTCCGCTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGAGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGCGCCCGAACAGGGACCTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGAAAAAAGCACAGCAAGCAGCAGCTGACACAGGACACAGCAATCAGGTCAGCCAAAATTACCCTATAGTGCAGAACATCCAGGGGCAAATGGTACATCAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGTAGAAGAGAAGGCTTTCAGCCCAGAAGTGATACCCATGTTTTCAGCATTATCAGAAGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGACATCAAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGAATGGGATAGAGTGCATCCAGTGCATGCAGGGCCTATTGCACCAGGCCAGATGAGAGAACCAAGGGGAAGTGACATAGCAGGAACTACTAGTACCCTTCAGGAACAAATAGGATGGATGACAAATAATCCACCTATCCCAGTAGGAGAAATTTATAAAAGATGGATAATCCTGGGATTAAATAAAATAGTAAGAATGTATAGCCCTACCAGCATTCTGGACATAAGACAAGGACCAAAGGAACCCTTTAGAGACTATGTAGACCGGTTCTATAAAACTCTAAGAGCCGAGCAAGCTTCACAGGAGGTAAAAAATTGGATGACAGAAACCTTGTTGGTCCAAAATGCGAACCCAGATTGTAAGACTATTTTAAAAGCATTGGGACCAGCGGCTACACTAGAAGAAATGATGACAGCATGTCAGGGAGTAGGAGGACCCGGCCATAAGGCAAGAGTTTTGGCTGAAGCAATGAGCCAAGTAACAAATTCAGCTACCATAATGATGCAGAGAGGCAATTTTAGGAACCAAAGAAAGATTGTTAAGTGTTTCAATTGTGGCAAAGAAGGGCACACAGCCAGAAATTGCAGGGCCCCTAGGAAAAAGGGCTGTTGGAAATGTGGAAAGGAAGGACACCAAATGAAAGATTGTACTGAGAGACAGGCTAATTTTTTAGGGAAGATCTGGCCTTCCTACAAGGGAAGGCCAGGGAATTTTCTTCAGAGCAGACCAGAGCCAACAGCCCCACCAGAAGAGAGCTTCAGGTCTGGGGTAGAGACAACAACTCCCCCTCAGAAGCAGGAGCCGATAGACAAGGAACTGTATCCTTTAACTTCCCTCAGGTCACTCTTTGGCAACGACCCCTCGTCACAATAAAGATAGGGGGGCAACTAAAGGAAGCTCTATTAGATACAGGAGCAGATGATACAGTATTAGAAGAAATGAGTTTGCCAGGAAGATGGAAACCAAAAATGATAGGGGGAATTGGAGGTTTTATCAAAGTAAGACAGTATGATCAGATACTCATAGAAATCTGTGGACATAAAGCTATAGGTACAGTATTAGTAGGACCTACACCTGTCAACATAATTGGAAGAAATCTGTTGACTCAGATTGGTTGCACTTTAAATTTTCCCATTAGCCCTATTGAGACTGTACCAGTAAAATTAAAGCCAGGAATGGATGGCCCAAAAGTTAAACAATGGCCATTGACAGAAGAAAAAATAAAAGCATTAGTAGAAATTTGTACAGAGATGGAAAAGGAAGGGAAAATTTCAAAAATTGGGCCTGAAAATCCATACAATACTCCAGTATTTGCCATAAAGAAAAAAGACAGTACTAAATGGAGAAAATTAGTAGATTTCAGAGAACTTAATAAGAGAACTCAAGACTTCTGGGAAGTTCAATTAGGAATACCACATCCCGCAGGGTTAAAAAAGAAAAAATCAGTAACAGTACTGGATGTGGGTGATGCATATTTTTCAGTTCCCTTAGATGAAGACTTCAGGAAGTATACTGCATTTACCATACCTAGTATAAACAATGAGACACCAGGGATTAGATATCAGTACAATGTGCTTCCACAGGGATGGAAAGGATCACCAGCAATATTCCAAAGTAGCATGACAAAAATCTTAGAGCCTTTTAGAAAACAAAATCCAGACATAGTTATCTATCAATACATGGATGATTTGTATGTAGGATCTGACTTAGAAATAGGGCAGCATAGAACAAAAATAGAGGAGCTGAGACAACATCTGTTGAGGTGGGGACTTACCACACCAGACAAAAAACATCAGAAAGAACCTCCATTCCTTTGGATGGGTTATGAACTCCATCCTGATAAATGGACAGTACAGCCTATAGTGCTGCCAGAAAAAGACAGCTGGACTGTCAATGACATACAGAAGTTAGTGGGGAAATTGAATTGGGCAAGTCAGATTTACCCAGGGATTAAAGTAAGGCAATTATGTAAACTCCTTAGAGGAACCAAAGCACTAACAGAAGTAATACCACTAACAGAAGAAGCAGAGCTAGAACTGGCAGAAAACAGAGAGATTCTAAAAGAACCAGTACATGGAGTGTATTATGACCCATCAAAAGACTTAATAGCAGAAATACAGAAGCAGGGGCAAGGCCAATGGACATATCAAATTTATCAAGAGCCATTTAAAAATCTGAAAACAGGAAAATATGCAAGAATGAGGGGTGCCCACACTAATGATGTAAAACAATTAACAGAGGCAGTGCAAAAAATAACCACAGAAAGCATAGTAATATGGGGAAAGACTCCTAAATTTAAACTGCCCATACAAAAGGAAACATGGGAAACATGGTGGACAGAGTATTGGCAAGCCACCTGGATTCCTGAGTGGGAGTTTGTTAATACCCCTCCCTTAGTGAAATTATGGTACCAGTTAGAGAAAGAACCCATAGTAGGAGCAGAAACCTTCTATGTAGATGGGGCAGCTAACAGGGAGACTAAATTAGGAAAAGCAGGATATGTTACTAATAGAGGAAGACAAAAAGTTGTCACCCTAACTGACACAACAAATCAGAAGACTGAGTTACAAGCAATTTATCTAGCTTTGCAGGATTCGGGATTAGAAGTAAACATAGTAACAGACTCACAATATGCATTAGGAATCATTCAAGCACAACCAGATCAAAGTGAATCAGAGTTAGTCAATCAAATAATAGAGCAGTTAATAAAAAAGGAAAAGGTCTATCTGGCATGGGTACCAGCACACAAAGGAATTGGAGGAAATGAACAAGTAGATAAATTAGTCAGTGCTGGAATCAGGAAAGTACTATTTTTAGATGGAATAGATAAGGCCCAAGATGAACATGAGAAATATCACAGTAATTGGAGAGCAATGGCTAGTGATTTTAACCTGCCACCTGTAGTAGCAAAAGAAATAGTAGCCAGCTGTGATAAATGTCAGCTAAAAGGAGAAGCCATGCATGGACAAGTAGACTGTAGTCCAGGAATATGGCAACTAGATTGTACACATTTAGAAGGAAAAGTTATCCTGGTAGCAGTTCATGTAGCCAGTGGATATATAGAAGCAGAAGTTATTCCAGCAGAAACAGGGCAGGAAACAGCATATTTTCTTTTAAAATTAGCAGGAAGATGGCCAGTAAAAACAATACATACTGACAATGGCAGCAATTTCACCGGTGCTACGGTTAGGGCCGCCTGTTGGTGGGCGGGAATCAAGCAGGAATTTGGAATTCCCTACAATCCCCAAAGTCAAGGAGTAGTAGAATCTATGAATAAAGAATTAAAGAAAATTATAGGACAGGTAAGAGATCAGGCTGAACATCTTAAGACAGCAGTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTACAGGGACAGCAGAAATCCACTTTGGAAAGGACCAGCAAAGCTCCTCTGGAAAGGTGAAGGGGCAGTAGTAATACAAGATAATAGTGACATAAAAGTAGTGCCAAGAAGAAAAGCAAAGATCATTAGGGATTATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAGGATTAGAACATGGAAAAGTTTAGTAAAACACCATATGTATGTTTCAGGGAAAGCTAGGGGATGGTTTTATAGACATCACTATGAAAGCCCTCATCCAAGAATAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTGGTAATAACAACATATTGGGGTCTGCATACAGGAGAAAGAGACTGGCATTTGGGTCAGGGAGTCTCCATAGAATGGAGGAAAAAGAGATATAGCACACAAGTAGACCCTGAACTAGCAGACCAACTAATTCATCTGTATTACTTTGACTGTTTTTCAGACTCTGCTATAAGAAAGGCCTTATTAGGACACATAGTTAGCCCTAGGTGTGAATATCAAGCAGGACATAACAAGGTAGGATCTCTACAATACTTGGCACTAGCAGCATTAATAACACCAAAAAAGATAAAGCCACCTTTGCCTAGTGTTACGAAACTGACAGAGGATAGATGGAACAAGCCCCAGAAGACCAAGGGCCACAGAGGGAGCCACACAATGAATGGACACTAGAGCTTTTAGAGGAGCTTAAGAATGAAGCTGTTAGACATTTTCCTAGGATTTGGCTCCATGGCTTAGGGCAACATATCTATGAAACTTATGGGGATACTTGGGCAGGAGTGGAAGCCATAATAAGAATTCTGCAACAACTGCTGTTTATCCATTTTCAGAATTGGGTGTCGACATAGCAGAATAGGCGTTACTCGACAGAGGAGAGCAAGAAATGGAGCCAGTAGATCCTAGACTAGAGCCCTGGAAGCATCCAGGAAGTCAGCCTAAAACTGCTTGTACCAATTGCTATTGTAAAAAGTGTTGCTTTCATTGCCAAGTTTGTTTCATAACAAAAGCCTTAGGCATCTCCTATGGCAGGAAGAAGCGGAGACAGCGACGAAGAGCTCATCAGAACAGTCAGACTCATCAAGCTTCTCTATCAAAGCAGTAAGTAGTACATGTAACGCAACCTATACCAATAGTAGCAATAGTAGCATTAGTAGTAGCAATAATAATAGCAATAGTTGTGTGGTCCATAGTAATCATAGAATATAGGAAAATATTAAGACAAAGAAAAATAGACAGGTTAATTGATAGACTAATAGAAAGAGCAGAAGACAGTGGCAATGAGAGTGAAGGAGAAATATCAGCACTTGTGGAGATGGGGGTGGAGATGGGGCACCATGCTCCTTGGGATGTTGATGATCTGTAGTGCTACAGAAAAATTGTGGGTCACAGTCTATTATGGGGTACCTGTGTGGAAGGAAGCAACCACCACTCTATTTTGTGCATCAGATGCTAAAGCATATGATACAGAGGTACATAATGTTTGGGCCACACATGCCTGTGTACCCACAGACCCCAACCCACAAGAAGTAGTATTGGTAAATGTGACAGAAAATTTTAACATGTGGAAAAATGACATGGTAGAACAGATGCATGAGGATATAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAATTAACCCCACTCTGTGTTAGTTTAAAGTGCACTGATTTGAAGAATGATACTAATACCAATAGTAGTAGCGGGAGAATGATAATGGAGAAAGGAGAGATAAAAAACTGCTCTTTCAATATCAGCACAAGCATAAGAGGTAAGGTGCAGAAAGAATATGCATTTTTTTATAAACTTGATATAATACCAATAGATAATGATACTACCAGCTATAAGTTGACAAGTTGTAACACCTCAGTCATTACACAGGCCTGTCCAAAGGTATCCTTTGAGCCAATTCCCATACATTATTGTGCCCCGGCTGGTTTTGCGATTCTAAAATGTAATAATAAGACGTTCAATGGAACAGGACCATGTACAAATGTCAGCACAGTACAATGTACACATGGAATTAGGCCAGTAGTATCAACTCAACTGCTGTTAAATGGCAGTCTAGCAGAAGAAGAGGTAGTAATTAGATCTGTCAATTTCACGGACAATGCTAAAACCATAATAGTACAGCTGAACACATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGAATCCGTATCCAGAGAGGACCAGGGAGAGCATTTGTTACAATAGGAAAAATAGGAAATATGAGACAAGCACATTGTAACATTAGTAGAGCAAAATGGAATAACACTTTAAAACAGATAGCTAGCAAATTAAGAGAACAATTTGGAAATAATAAAACAATAATCTTTAAGCAATCCTCAGGAGGGGACCCAGAAATTGTAACGCACAGTTTTAATTGTGGAGGGGAATTTTTCTACTGTAATTCAACACAACTGTTTAATAGTACTTGGTTTAATAGTACTTGGAGTACTGAAGGGTCAAATAACACTGAAGGAAGTGACACAATCACCCTCCCATGCAGAATAAAACAAATTATAAACATGTGGCAGAAAGTAGGAAAAGCAATGTATGCCCCTCCCATCAGTGGACAAATTAGATGTTCATCAAATATTACAGGGCTGCTATTAACAAGAGATGGTGGTAATAGCAACAATGAGTCCGAGATCTTCAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAATAAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAACAATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAATGAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACAAATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGAATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCGTTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAAGGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCCTTGGCACTTATCTGGGACGATCTGCGGAGCCTGTGCCTCTTCAGCTACCACCGCTTGAGAGACTTACTCTTGATTGTAACGAGGATTGTGGAACTTCTGGGACGCAGGGGGTGGGAAGCCCTCAAATATTGGTGGAATCTCCTACAGTATTGGAGTCAGGAACTAAAGAATAGTGCTGTTAGCTTGCTCAATGCCACAGCCATAGCAGTAGCTGAGGGGACAGATAGGGTTATAGAAGTAGTACAAGGAGCTTGTAGAGCTATTCGCCACATACCTAGAAGAATAAGACAGGGCTTGGAAAGGATTTTGCTATAAGATGGGTGGCAAGTGGTCAAAAAGTAGTGTGATTGGATGGCCTACTGTAAGGGAAAGAATGAGACGAGCTGAGCCAGCAGCAGATAGGGTGGGAGCAGCATCTCGAGACCTGGAAAAACATGGAGCAATCACAAGTAGCAATACAGCAGCTACCAATGCTGCTTGTGCCTGGCTAGAAGCACAAGAGGAGGAGGAGGTGGGTTTTCCAGTCACACCTCAGGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAAAGAAGACAAGATATCCTTGATCTGTGGATCTACCACACACAAGGCTACTTCCCTGATTAGCAGAACTACACACCAGGGCCAGGGGTCAGATATCCACTGACCTTTGGATGGTGCTACAAGCTAGTACCAGTTGAGCCAGATAAGATAGAAGAGGCCAATAAAGGAGAGAACACCAGCTTGTTACACCCTGTGAGCCTGCATGGGATGGATGACCCGGAGAGAGAAGTGTTAGAGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACGTGGCCCGAGAGCTGCATCCGGAGTACTTCAAGAACTGCTGACATCGAGCTTGCTACAAGGGACTTTCCGCTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGAGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCA diff -r 000000000000 -r f41a1e03538b tool-data/all_fasta.loc.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/all_fasta.loc.sample Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,18 @@ +#This file lists the locations and dbkeys of all the fasta files +#under the "genome" directory (a directory that contains a directory +#for each build). The script extract_fasta.py will generate the file +#all_fasta.loc. This file has the format (white space characters are +#TAB characters): +# +# +# +#So, all_fasta.loc could look something like this: +# +#apiMel3 apiMel3 Honeybee (Apis mellifera): apiMel3 /path/to/genome/apiMel3/apiMel3.fa +#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /path/to/genome/hg19/hg19canon.fa +#hg19full hg19 Human (Homo sapiens): hg19 Full /path/to/genome/hg19/hg19full.fa +# +#Your all_fasta.loc file should contain an entry for each individual +#fasta file. So there will be multiple fasta files for each build, +#such as with hg19 above. +# diff -r 000000000000 -r f41a1e03538b tool-data/tool_data_table_conf.xml.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/tool_data_table_conf.xml.sample Wed Oct 30 15:33:59 2019 -0400 @@ -0,0 +1,7 @@ + + + + value, dbkey, name, path + +
+