Mercurial > repos > iuc > gemini_amend
diff test-data/util/build-gemini-testdata.sh @ 4:a02cd62adb70 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gemini commit 283362494058ed64143b1f27afb447b8a1cb4313
author | iuc |
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date | Fri, 14 Dec 2018 12:47:55 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/util/build-gemini-testdata.sh Fri Dec 14 12:47:55 2018 -0500 @@ -0,0 +1,72 @@ +cd "$(dirname "$0")" + +export GEMINI_CONFIG=../test-cache +OUT_PTH=$GEMINI_CONFIG/gemini/data +GENOMIC_REGION=3:187000000-187500000 + + +if [ -n "$1" ]; then + +IN_PTH="$1" +# downsample all vcf and bed annotation files to the region of interest and reindex +for vcf in `ls $IN_PTH/*.gz | grep -v hprd_interaction_edges.gz -` +do + python ./shrink_tabix.py $vcf -r $GENOMIC_REGION -o $OUT_PTH/`basename $vcf` +done + +# downsample gene_table files to the region of interest +echo "$IN_PTH/summary_gene_table_v75 -> $OUT_PTH/summary_gene_table_v75" +python ./shrink_simple_tab.py $IN_PTH/summary_gene_table_v75 -r chr$GENOMIC_REGION -c 0 8 9 -n 1 -o $OUT_PTH/summary_gene_table_v75 + +echo "$IN_PTH/detailed_gene_table_v75 -> $OUT_PTH/detailed_gene_table_v75" +python ./shrink_simple_tab.py $IN_PTH/detailed_gene_table_v75 -r chr$GENOMIC_REGION -c 0 11 12 -n 1 -o $OUT_PTH/detailed_gene_table_v75 + +# filter kegg_pathway files to retain only records of the genes listed +# in the downsampled summary_gene_table +for kegg in `ls $IN_PTH/kegg_pathways_*` +do + echo "$kegg -> $OUT_PTH/`basename $kegg`" + cut -f2 $OUT_PTH/summary_gene_table_v75 | grep -Fv None | grep -Fwf - $kegg > $OUT_PTH/`basename $kegg` +done + +# filter hprd_interaction file to retain only records of the genes listed +# in the downsampled summary_gene_table +echo "$IN_PTH/hprd_interaction_edges.gz -> $OUT_PTH/hprd_interaction_edges.gz" +bgzip -dc $IN_PTH/hprd_interaction_edges.gz > $OUT_PTH/hprd_interaction_edges +cut -f2 $OUT_PTH/summary_gene_table_v75 | grep -Fv None | grep -Ff - $OUT_PTH/hprd_interaction_edges | bgzip > $OUT_PTH/hprd_interaction_edges.gz +rm $OUT_PTH/hprd_interaction_edges + +# filter cancer_gene_census file to retain only records of the genes listed +# in the downsampled summary_gene_table; +# TO DO: make the filter stricter by looking for matches only in the first +# column of the cancer_gene_census file (but the file is relatively small anyway) +echo "$IN_PTH/cancer_gene_census.20140120.tsv -> $OUT_PTH/cancer_gene_census.20140120.tsv" +cut -f2 $OUT_PTH/summary_gene_table_v75 | grep -Fv None | grep -Fwf - $IN_PTH/cancer_gene_census.20140120.tsv > $OUT_PTH/cancer_gene_census.20140120.tsv + +else + echo "no path to gemini annotation files provided - only building test databases" +fi + + +# now use gemini load to build the test databases +echo "Building gemini test databases" +echo "Test databases for gemini_load" +gemini --annotation-dir $OUT_PTH load --skip-cadd --skip-gerp-bp -v build-data/gemini_load_input.vcf -t snpEff ../gemini_load_result1.db +gemini --annotation-dir $OUT_PTH load --skip-cadd --skip-gerp-bp -v build-data/gemini_load_input.vcf -t snpEff --skip-gene-tables --no-load-genotypes ../gemini_load_result2.db +echo "Test database for gemini_amend" +gemini --annotation-dir $OUT_PTH load --skip-cadd --skip-gerp-bp -v build-data/test.auto_rec.vcf -t snpEff ../gemini_amend_input.db +echo "Test database for gemini_annotate" +bgzip -c build-data anno.bed > build-data/anno.bed.gz +tabix --force -p bed build-data/anno.bed.gz +cp ../gemini_load_result1.db ../gemini_annotate_result.db +gemini --annotation-dir $OUT_PTH annotate -f build-data/anno.bed.gz -c anno5 -a count ../gemini_annotate_result.db +echo "Test database for gemini_set_somatic" +cp ../gemini_load_result1.db ../gemini_is_somatic_result.db +gemini set_somatic --min-somatic-score 5.65 ../gemini_is_somatic_result.db +echo "Test database for gemini_de_novo and gemini_mendel_errors" +gemini --annotation-dir $OUT_PTH load --skip-cadd --skip-gerp-bp -v build-data/test.de_novo.vcf -p build-data/test.de_novo.ped -t snpEff ../gemini_de_novo_input.db +echo "Test database for gemini_comp_hets" +gemini --annotation-dir $OUT_PTH load --skip-cadd --skip-gerp-bp -v build-data/test.comp_het.vcf -p build-data/test.comp_het.ped -t snpEff ../gemini_comphets_input.db +echo "Test databases for gemini_autosomal" +gemini --annotation-dir $OUT_PTH load --skip-cadd --skip-gerp-bp -v build-data/test.auto_rec.vcf -p build-data/test.auto_rec.ped -t snpEff ../gemini_auto_rec_input.db +gemini --annotation-dir $OUT_PTH load --skip-cadd --skip-gerp-bp -v build-data/test.auto_dom.vcf -p build-data/test.auto_dom.ped -t snpEff ../gemini_auto_dom_input.db