Mercurial > repos > iuc > homer_annotatepeaks
diff test-data/generate_tests.sh @ 0:adab852c8216 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/homer commit e49d856e0181edc6120220a1b819cba2466a4289"
author | iuc |
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date | Sun, 08 Aug 2021 11:03:29 +0000 |
parents | |
children | 35dc2f57d755 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/generate_tests.sh Sun Aug 08 11:03:29 2021 +0000 @@ -0,0 +1,59 @@ +#! /usr/bin/bash +## Generate input data: +if [ ! -e test-data/small_simplified.gtf ]; then + wget http://ftp.ensembl.org/pub/release-102/gtf/mus_musculus/Mus_musculus.GRCm38.102.gtf.gz -O /tmp/Mus_musculus.GRCm38.102.gtf.gz + zcat /tmp/Mus_musculus.GRCm38.102.gtf.gz | head -n 5 > test-data/small.gtf + zcat /tmp/Mus_musculus.GRCm38.102.gtf.gz | awk -v OFS="\t" -v start=74667792 -v end=74748393 '$1 == "2" && $5 > start && $4 < end{print "chr"$0}' >> test-data/small.gtf + # annotatePeaks.pl gives different results all time. I need to simplify the gtf. + cat test-data/small.gtf | grep -v -P "ENSMUST00000152027|ENSMUST00000156342|ENSMUST00000139005|ENSMUST00000144544|ENSMUST00000111982|ENSMUST00000140666|ENSMUST00000190553|ENSMUST00000132326|ENSMUST00000047830|ENSMUST00000047904|ENSMUST00000111980|ENSMUSG00000065500|ENSMUSG00000100642" > test-data/small_simplified.gtf +fi +if [ ! -e test-data/CTCF_peaks.bed ]; then + wget https://raw.githubusercontent.com/lldelisle/scriptsForWilleminEtAl2021/main/CTCF/E12_Limbs_Wt_CTCF_colored.bed -O test-data/CTCF_peaks.bed +fi +if [ ! -e test-data/CTCF_peaks_shifted.bed ]; then + cat test-data/CTCF_peaks.bed | grep "chr2" | awk -v OFS="\t" '{$1="mm10_dna"; $2-=73740000; $3-=73740000; print}' > test-data/CTCF_peaks_shifted.bed +fi +# chr2_subset.fa was downloaded from UCSC +# https://genome.ucsc.edu/cgi-bin/hgc?hgsid=1136019667_XgAJOvV4a3CY4ibCu6RrUcvGxLNo&g=htcGetDna2&table=&i=mixed&o=56694975&l=56694975&r=56714605&getDnaPos=chr2%3A73740000-75787000&db=mm10&hgSeq.cdsExon=1&hgSeq.padding5=0&hgSeq.padding3=0&hgSeq.casing=upper&hgSeq.maskRepeats=on&boolshad.hgSeq.maskRepeats=0&hgSeq.repMasking=lower&boolshad.hgSeq.revComp=0&submit=get+DNA + +. <(planemo conda_env homer_gtf_to_annotation.xml) +echo "$(which homer)" +## homer_gtf_to_annotation +## First test +parseGTF.pl test-data/small.gtf ann -features exon start_codon stop_codon > /tmp/annotations.txt +assignGenomeAnnotation /tmp/annotations.txt /tmp/annotations.txt -prioritize test-data/annotations_default.txt > test-data/annotations_default_stats.txt +## Second test +parseGTF.pl test-data/small.gtf ann -features exon start_codon > /tmp/annotations2.txt +assignGenomeAnnotation /tmp/annotations2.txt /tmp/annotations2.txt -prioritize test-data/annotations_exon_start.txt > test-data/annotations_exon_start_stats.txt +## Third test +parseGTF.pl test-data/small.gtf ann -features exon start_codon stop_codon -annTSSstartOffset -50 -annTSSendOffset 50 -annTTSstartOffset -50 -annTTSendOffset 50 > /tmp/annotations3.txt +assignGenomeAnnotation /tmp/annotations3.txt /tmp/annotations3.txt -prioritize test-data/annotations_small_TSSTTS.txt > test-data/annotations_small_TSSTTS_stats.txt + +## For annotatePeaks.pl +parseGTF.pl test-data/small_simplified.gtf ann -features exon start_codon stop_codon > /tmp/annotations.txt +assignGenomeAnnotation /tmp/annotations.txt /tmp/annotations.txt -prioritize test-data/annotations_default_simplified.txt + +## homer_annotatePeaks +## First test +annotatePeaks.pl test-data/CTCF_peaks.bed none -gtf test-data/small_simplified.gtf -ann test-data/annotations_default_simplified.txt > test-data/CTCF_peaks_first.txt +## Second test +annotatePeaks.pl test-data/CTCF_peaks.bed none -ann test-data/annotations_default.txt > test-data/CTCF_peaks_second.txt +## Third test +annotatePeaks.pl test-data/CTCF_peaks.bed none -gtf test-data/small_simplified.gtf > test-data/CTCF_peaks_third.txt +## Fourth test +annotatePeaks.pl test-data/fake_phix_peaks.bed test-data/phiX174.fasta -CpG > test-data/phiXcpg.txt +## Fifth test +annotatePeaks.pl test-data/fake_phix_peaks.bed none > test-data/phiX_nothing.txt + +## findMotifsGenome +# ! Genome preparsing is giving different results... +findMotifsGenome.pl test-data/fake_phix_peaks.bed test-data/phiX174.fasta fake_phix_peaks_bed_motif +mv fake_phix_peaks_bed_motif test-data/motif_test1 +# Thus I needed to use has_text for the other outputs +# findMotifsGenome.pl test-data/CTCF_peaks_shifted.bed test-data/chr2_subset.fa CTCF_peaks_shifted_bed_motif +# mv CTCF_peaks_shifted_bed_motif test-data/motif_test2 +# findMotifsGenome.pl test-data/CTCF_peaks_shifted.bed test-data/chr2_subset.fa CTCF_peaks_shifted_bed_motif -mask +# mv CTCF_peaks_shifted_bed_motif test-data/motif_test3 +# findMotifsGenome.pl test-data/CTCF_peaks_shifted.bed test-data/chr2_subset.fa CTCF_peaks_shifted_bed_motif -mset plants -nomotif +# mv CTCF_peaks_shifted_bed_motif test-data/motif_test4 +