Mercurial > repos > iuc > homer_gtf_to_annotations

annotate test-data/generate_tests.sh @ 4:df55927535ed draft default tip

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/homer commit e6639e130c5bf50f489dd09e38a1cbc712280fff
author iuc
date Fri, 07 Apr 2023 15:02:25 +0000
parents 396cf2c12173
children
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1 #! /usr/bin/bash
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2 ## Generate input data:
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3 if [ ! -e test-data/small_simplified.gtf ]; then
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4 wget http://ftp.ensembl.org/pub/release-102/gtf/mus_musculus/Mus_musculus.GRCm38.102.gtf.gz -O /tmp/Mus_musculus.GRCm38.102.gtf.gz
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5 zcat /tmp/Mus_musculus.GRCm38.102.gtf.gz | head -n 5 > test-data/small.gtf
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6 zcat /tmp/Mus_musculus.GRCm38.102.gtf.gz | awk -v OFS="\t" -v start=74667792 -v end=74748393 '$1 == "2" && $5 > start && $4 < end{print "chr"$0}' >> test-data/small.gtf
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7 # annotatePeaks.pl gives different results all time. I need to simplify the gtf.
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8 cat test-data/small.gtf | grep -v -P "ENSMUST00000152027|ENSMUST00000156342|ENSMUST00000139005|ENSMUST00000144544|ENSMUST00000111982|ENSMUST00000140666|ENSMUST00000190553|ENSMUST00000132326|ENSMUST00000047830|ENSMUST00000047904|ENSMUST00000111980|ENSMUSG00000065500|ENSMUSG00000100642" > test-data/small_simplified.gtf
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9 fi
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10 if [ ! -e test-data/CTCF_peaks.bed ]; then
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11 wget https://raw.githubusercontent.com/lldelisle/scriptsForWilleminEtAl2021/main/CTCF/E12_Limbs_Wt_CTCF_colored.bed -O test-data/CTCF_peaks.bed
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12 fi
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13 if [ ! -e test-data/CTCF_peaks_shifted.bed ]; then
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14 cat test-data/CTCF_peaks.bed | grep "chr2" | awk -v OFS="\t" '$3<75000000 && $2>73740000{$1="mm10_dna"; $2-=73740000; $3-=73740000; print}' > test-data/CTCF_peaks_shifted.bed
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15 fi
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16 # chr2_subset.fa was downloaded from UCSC
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17 # https://genome.ucsc.edu/cgi-bin/hgc?hgsid=1234982067_JnS4z30UVCNarTg26Ztd1Oh6nfu6&g=htcGetDna2&table=&i=mixed&o=56694975&l=56694975&r=56714605&getDnaPos=chr2%3A73740000-75000000&db=mm10&hgSeq.cdsExon=1&hgSeq.padding5=0&hgSeq.padding3=0&hgSeq.casing=upper&hgSeq.maskRepeats=on&boolshad.hgSeq.maskRepeats=0&hgSeq.repMasking=lower&boolshad.hgSeq.revComp=0&submit=get+DNA
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19 . <(planemo conda_env homer_gtf_to_annotation.xml)
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20 echo "$(which homer)"
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21 ## homer_gtf_to_annotation
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22 ## First test
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23 parseGTF.pl test-data/small.gtf ann -features exon start_codon stop_codon > /tmp/annotations.txt
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24 assignGenomeAnnotation /tmp/annotations.txt /tmp/annotations.txt -prioritize test-data/annotations_default.txt > test-data/annotations_default_stats.txt
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25 ## Second test
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26 parseGTF.pl test-data/small.gtf ann -features exon start_codon > /tmp/annotations2.txt
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27 assignGenomeAnnotation /tmp/annotations2.txt /tmp/annotations2.txt -prioritize test-data/annotations_exon_start.txt > test-data/annotations_exon_start_stats.txt
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28 ## Third test
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29 parseGTF.pl test-data/small.gtf ann -features exon start_codon stop_codon -annTSSstartOffset -50 -annTSSendOffset 50 -annTTSstartOffset -50 -annTTSendOffset 50 > /tmp/annotations3.txt
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30 assignGenomeAnnotation /tmp/annotations3.txt /tmp/annotations3.txt -prioritize test-data/annotations_small_TSSTTS.txt > test-data/annotations_small_TSSTTS_stats.txt
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32 ## For annotatePeaks.pl
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33 parseGTF.pl test-data/small_simplified.gtf ann -features exon start_codon stop_codon > /tmp/annotations.txt
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34 assignGenomeAnnotation /tmp/annotations.txt /tmp/annotations.txt -prioritize test-data/annotations_default_simplified.txt
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36 ## homer_annotatePeaks
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37 ## First test
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38 annotatePeaks.pl test-data/CTCF_peaks.bed none -gtf test-data/small_simplified.gtf -ann test-data/annotations_default_simplified.txt > test-data/CTCF_peaks_first.txt
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39 ## Second test
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40 annotatePeaks.pl test-data/CTCF_peaks.bed none -ann test-data/annotations_default.txt > test-data/CTCF_peaks_second.txt
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41 ## Third test
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42 annotatePeaks.pl test-data/CTCF_peaks.bed none -gtf test-data/small_simplified.gtf > test-data/CTCF_peaks_third.txt
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43 ## Fourth test
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44 annotatePeaks.pl test-data/fake_phix_peaks.bed test-data/phiX174.fasta -CpG > test-data/phiXcpg.txt
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45 ## Fifth test
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46 annotatePeaks.pl test-data/fake_phix_peaks.bed none > test-data/phiX_nothing.txt
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48 ## findMotifsGenome
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49 # ! Genome preparsing is giving different results...
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50 findMotifsGenome.pl test-data/fake_phix_peaks.bed test-data/phiX174.fasta fake_phix_peaks_bed_motif
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51 mv fake_phix_peaks_bed_motif test-data/motif_test1
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52 # Thus I needed to use has_text for the other outputs
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53 # gunzip -c test-data/chr2_subset.fa.gz > test-data/chr2_subset.fa
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54 # findMotifsGenome.pl test-data/CTCF_peaks_shifted.bed test-data/chr2_subset.fa CTCF_peaks_shifted_bed_motif
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55 # mv CTCF_peaks_shifted_bed_motif test-data/motif_test2
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56 # findMotifsGenome.pl test-data/CTCF_peaks_shifted.bed test-data/chr2_subset.fa CTCF_peaks_shifted_bed_motif -mask
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57 # mv CTCF_peaks_shifted_bed_motif test-data/motif_test3
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58 # findMotifsGenome.pl test-data/CTCF_peaks_shifted.bed test-data/chr2_subset.fa CTCF_peaks_shifted_bed_motif -mset plants -nomotif
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59 # mv CTCF_peaks_shifted_bed_motif test-data/motif_test4
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