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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/homer commit e6639e130c5bf50f489dd09e38a1cbc712280fff
author | iuc |
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date | Fri, 07 Apr 2023 15:02:25 +0000 |
parents | 7c5da353e460 |
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<tool id="homer_gtf_to_annotations" name="Converts GTF to Annotations file for Homer" version="@TOOL_VERSION@+galaxy0" profile="@PROFILE@"> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"/> <command detect_errors="exit_code"><![CDATA[ ## Run parseGTF.pl parseGTF.pl $gtf_file ann ## Different options $type $id $removeAccVer $removeEnsemblVer -annTSSstartOffset $annTSSstartOffset -annTSSendOffset $annTSSendOffset -annTTSstartOffset $annTTSstartOffset -annTTSendOffset $annTTSendOffset #if $features != 'None': #set features_space = ' '.join(str($features).split(',')) -features $features_space #end if > annotations.txt && ## Prioritize and add intergenic annotation assignGenomeAnnotation annotations.txt annotations.txt -prioritize $output > $output_stats ]]></command> <inputs> <param name="gtf_file" type="data" format="gtf,gff,gff3" label="GTF file" help="Must be of datatype "gtf" or "gff" or "gff3""/> <param name="type" type="select" label="Format of input file"> <option value="">GTF</option> <option value="-gff">GFF (treats 9th column as ID)</option> <option value="-gff3">GFF3 (looks for parent attribute to assign gene name)</option> </param> <param name="id" type="select" label="Primary identifier"> <option value="-tid">Transcript ID</option> <option value="-gid">Gene ID</option> </param> <param name="features" type="select" label="Which feature(s) to use?" multiple="true" display="checkboxes"> <option value="exon" selected="true">exon</option> <option value="start_codon" selected="true">start_codon</option> <option value="stop_codon" selected="true">stop_codon</option> </param> <param name="removeAccVer" type="boolean" truevalue="-removeAccVer" falsevalue="" label="Remove any .1, .2, etc. at end of accession numbers, i.e. AT1G01040.2" help="-removeAccVer; Default=False"/> <param name="removeEnsemblVer" type="boolean" truevalue="-removeEnsemblVer" falsevalue="" label="Remove 'transcript:' and '_T01' style ids" help="-removeEnsemblVer; Default=False"/> <param name="annTSSstartOffset" type="integer" value="-1000" label="distance upstream of TSS to start promoter annotation" help="-annTSSstartOffset; Default=-1000"/> <param name="annTSSendOffset" type="integer" value="100" label="distance upstream/downstream of TSS to end promoter annotation" help="-annTSSendOffset; Default=100"/> <param name="annTTSstartOffset" type="integer" value="-100" label="distance upstream of TTS to start TTS annotation" help="-annTTSstartOffset; Default=-100"/> <param name="annTTSendOffset" type="integer" value="1000" label="distance upstream/downstream of TTS to end TTS annotation" help="-annTTSendOffset; Default=1000"/> </inputs> <outputs> <data format="tabular" name="output" label="${tool.name} on ${on_string}: Annotations"/> <data format="tabular" name="output_stats" label="${tool.name} on ${on_string}: Annotation stats"/> </outputs> <tests> <test> <!-- simple test with default --> <param name="gtf_file" value="small.gtf" ftype="gtf"/> <output name="output" file="annotations_default.txt" ftype="tabular"/> <output name="output_stats" file="annotations_default_stats.txt" ftype="tabular"/> </test> <test> <!-- simple test with default except no exon_end --> <param name="gtf_file" value="small.gtf" ftype="gtf"/> <param name="features" value="exon,start_codon"/> <output name="output" file="annotations_exon_start.txt" ftype="tabular"/> <output name="output_stats" file="annotations_exon_start_stats.txt" ftype="tabular"/> </test> <test> <!-- simple test with default smaller TSS TTS --> <param name="gtf_file" value="small.gtf" ftype="gtf"/> <param name="annTSSstartOffset" value="-50"/> <param name="annTSSendOffset" value="50"/> <param name="annTTSstartOffset" value="-50"/> <param name="annTTSendOffset" value="50"/> <output name="output" file="annotations_small_TSSTTS.txt" ftype="tabular"/> <output name="output_stats" file="annotations_small_TSSTTS_stats.txt" ftype="tabular"/> </test> </tests> <help><![CDATA[ This tool convert a GTF file to a HOMER-style annotation file compatible with annotatePeaks. It follows the tutorial proposed here: http://homer.ucsd.edu/homer/ngs/advancedAnnotation.html ]]></help> <expand macro="citation"/> </tool>