--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/customizemapping.py	Wed May 12 09:00:09 2021 +0000
@@ -0,0 +1,47 @@
+#!/usr/bin/env python
+# -*- coding: utf-8 -*-
+
+import argparse
+from pathlib import Path
+
+
+if __name__ == '__main__':
+    # Read command line
+    parser = argparse.ArgumentParser(description='Customize HUMAnN utility mapping')
+    parser.add_argument('--in_mapping', help="Path to mapping file to reduce")
+    parser.add_argument('--features', help="Path to tabular file with features to keep in first column")
+    parser.add_argument('--elements', help="Path to tabular file with elements to keep in other columns")
+    parser.add_argument('--out_mapping', help="Path to reduced mapping file")
+    args = parser.parse_args()
+
+    in_mapping_fp = Path(args.in_mapping)
+    feature_fp = Path(args.features)
+    element_fp = Path(args.elements)
+    out_mapping_fp = Path(args.out_mapping)
+
+    # extract features to keep
+    features = set()
+    with open(feature_fp, 'r') as feature_f:
+        for line in feature_f.readlines():
+            features.add(line.split("\t")[0])
+    print(features)
+
+    # extract elements to keep
+    elements = set()
+    with open(element_fp, 'r') as element_f:
+        for line in element_f.readlines():
+            elements.add(line.split("\t")[0])
+    print(elements)
+
+    # write mapping for features to keep while keeping only elements
+    with open(in_mapping_fp, 'r') as in_mapping_f:
+        with open(out_mapping_fp, 'w') as out_mapping_f:
+            for line in in_mapping_f.readlines():
+                l_split = line.split("\t")
+                feat = l_split[0]
+                if feat in features:
+                    to_write = [feat]
+                    for e in l_split[1:]:
+                        if e in elements:
+                            to_write.append(e)
+                    out_mapping_f.write("%s\n" % '\t'.join(to_write))

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/customizemetadata.py	Wed May 12 09:00:09 2021 +0000
@@ -0,0 +1,480 @@
+#!/usr/bin/env python
+# -*- coding: utf-8 -*-
+
+import argparse
+import bz2
+import json
+import pickle
+import re
+from pathlib import Path
+
+
+def load_from_json(json_fp):
+    '''
+    Read JSON file with marker metadata
+
+    :param json_fp: Path to JSON file
+    '''
+    with open(json_fp, 'r') as json_f:
+        data = json.load(json_f)
+
+    for m in data['markers']:
+        data['markers'][m]['ext'] = set(data['markers'][m]['ext'])
+
+    for t in data['taxonomy']:
+        if isinstance(data['taxonomy'][t], list):
+            data['taxonomy'][t] = tuple(data['taxonomy'][t])
+    return data
+
+
+def dump_to_json(data, json_fp):
+    '''
+    Dump marker metadata to JSON file
+
+    :param json_fp: Path to JSON file
+    '''
+    for m in data['markers']:
+        data['markers'][m]['ext'] = list(data['markers'][m]['ext'])
+
+    with open(json_fp, 'w') as json_f:
+        json.dump(data, json_f)
+
+
+def transform_pkl_to_json(pkl_fp, json_fp):
+    '''
+    Read Pickle file and drop it to a JSON file
+
+    :param pkl_fp: Path to input Pickle file
+    :param json_fp: Path to output JSON file
+    '''
+    # load metadata from Pickle file
+    with bz2.BZ2File(pkl_fp, 'r') as pkl_f:
+        in_metadata = pickle.load(pkl_f)
+
+    out_metadata = {
+        'markers': in_metadata['markers'],
+        'taxonomy': in_metadata['taxonomy'],
+        'merged_taxon': {}
+    }
+    # transform merged_taxons tuple keys to string
+    for k in in_metadata['merged_taxon']:
+        n = ' , '.join(k)
+        out_metadata[n] = in_metadata['merged_taxon'][k]
+
+    # dump metadata to JSON file
+    dump_to_json(out_metadata, json_fp)
+
+
+def transform_json_to_pkl(json_fp, pkl_fp):
+    '''
+    Read JSON file and drop it to a Pickle file
+
+    :param json_fp: Path to input JSON file
+    :param pkl_fp: Path to output Pickle file
+    '''
+    # load metadata from JSON file
+    in_metadata = load_from_json(json_fp)
+
+    out_metadata = {
+        'markers': in_metadata['markers'],
+        'taxonomy': in_metadata['taxonomy'],
+        'merged_taxon': {}
+    }
+    # transform merged_taxons keys to tuple
+    for k in in_metadata['merged_taxon']:
+        n = ' , '.split(k)
+        out_metadata[n] = in_metadata['merged_taxon'][k]
+
+    # dump metadata to Pickle file
+    with bz2.BZ2File(pkl_fp, 'w') as pkl_f:
+        pickle.dump(out_metadata, pkl_f)
+
+
+def add_marker(in_json_fp, out_json_fp, name, m_length, g_length, gca, k_name, k_id, p_name, p_id, c_name, c_id, o_name, o_id, f_name, f_id, g_name, g_id, s_name, s_id, t_name):
+    '''
+    Add marker to JSON file
+
+    :param in_json_fp: Path to input JSON file
+    :param out_json_fp: Path to output JSON file
+    :param name: Name of new marker
+    :param m_length: Length of new marker
+    :param g_length: List with lengths of genomes from which the new marker has been extracted
+    :param gca: List with GCA of genomes from which the new marker has been extracted
+    :param k_name: List with Name of Kingdom for genomes from which the new marker has been extracted
+    :param k_id: List with NCBI id of Kingdom for genomes from which the new marker has been extracted
+    :param p_name: List with Name of Phylum for genomes from which the new marker has been extracted
+    :param p_id: List with NCBI id of Phylum for genomes from which the new marker has been extracted
+    :param c_name: List with Name of Class for genomes from which the new marker has been extracted
+    :param c_id: List with NCBI id of Class for genomes from which the new marker has been extracted
+    :param o_name: List with Name of Order for genomes from which the new marker has been extracted
+    :param o_id: List with NCBI id of Order for genomes from which the new marker has been extracted
+    :param f_name: List with Name of Family for genomes from which the new marker has been extracted
+    :param f_id: List with NCBI id of Family for genomes from which the new marker has been extracted
+    :param g_name: List with Name of Genus for genomes from which the new marker has been extracted
+    :param g_id: List with NCBI id of Genus for genomes from which the new marker has been extracted
+    :param s_name: List with Name of Species for genomes from which the new marker has been extracted
+    :param s_id: List with NCBI id of Species for genomes from which the new marker has been extracted
+    :param t_name: List with Name of Strain for genomes from which the new marker has been extracted
+    '''
+    metadata = load_from_json(in_json_fp)
+
+    # check that all lists have same size
+    genome_n = len(g_length)
+    if len(gca) != genome_n:
+        raise ValueError("Missing/Extra values in GCA list")
+    if len(k_name) != genome_n:
+        raise ValueError("Missing/Extra values in Kingdom name list")
+    if len(k_id) != genome_n:
+        raise ValueError("Missing/Extra values in Kingdom ID list")
+    if len(p_name) != genome_n:
+        raise ValueError("Missing/Extra values in Phylum name list")
+    if len(p_id) != genome_n:
+        raise ValueError("Missing/Extra values in Phylum ID list")
+    if len(c_name) != genome_n:
+        raise ValueError("Missing/Extra values in Class name list")
+    if len(c_id) != genome_n:
+        raise ValueError("Missing/Extra values in Class ID list")
+    if len(o_name) != genome_n:
+        raise ValueError("Missing/Extra values in Order name list")
+    if len(o_id) != genome_n:
+        raise ValueError("Missing/Extra values in Order ID list")
+    if len(f_name) != genome_n:
+        raise ValueError("Missing/Extra values in Family name list")
+    if len(f_id) != genome_n:
+        raise ValueError("Missing/Extra values in Family ID list")
+    if len(g_name) != genome_n:
+        raise ValueError("Missing/Extra values in Genus name list")
+    if len(g_id) != genome_n:
+        raise ValueError("Missing/Extra values in Genus ID list")
+    if len(s_name) != genome_n:
+        raise ValueError("Missing/Extra values in Species name list")
+    if len(s_id) != genome_n:
+        raise ValueError("Missing/Extra values in Species ID list")
+    if len(t_name) != genome_n:
+        raise ValueError("Missing/Extra values in Strain name list")
+
+    # create dictionary to aggregate genome taxonomies and identify marker taxonomy
+    taxonomy = {
+        'k': set(),
+        'p': set(),
+        'c': set(),
+        'o': set(),
+        'f': set(),
+        'g': set(),
+        's': set(),
+        't': set(),
+    }
+
+    # parse genomes
+    for i in range(genome_n):
+        # add taxonomy of new genome
+        g_taxo_names = "k__%s|p__%s|c__%s|o__%s|f__%s|g__%s|s__%s|t__%s" % (
+            k_name[i],
+            p_name[i],
+            c_name[i],
+            o_name[i],
+            f_name[i],
+            g_name[i],
+            s_name[i],
+            t_name[i]
+        )
+        g_taxo_ids = "%s|%s|%s|%s|%s|%s|%s" % (
+            k_id[i],
+            p_id[i],
+            c_id[i],
+            o_id[i],
+            f_id[i],
+            g_id[i],
+            s_id[i]
+        )
+        metadata['taxonomy'][g_taxo_names] = (g_taxo_ids, g_length[i])
+        # aggregate taxon levels using sets
+        taxonomy['k'].add(k_name[i])
+        taxonomy['p'].add(p_name[i])
+        taxonomy['c'].add(c_name[i])
+        taxonomy['o'].add(o_name[i])
+        taxonomy['f'].add(f_name[i])
+        taxonomy['g'].add(g_name[i])
+        taxonomy['s'].add(s_name[i])
+        taxonomy['t'].add(t_name[i])
+
+    # extract clade and taxon of marker
+    clade = ''  # last level before taxomy of genomes diverge
+    taxon = ''  # combination of levels before divergence
+    for level in ['k', 'p', 'c', 'o', 'f', 'g', 's', 't']:
+        taxo = list(taxonomy[level])
+        if len(taxo) == 1:
+            clade = taxo[0]
+            taxon = "%s|%s__%s" % (taxon, level, taxo)
+
+    # add information about the new marker
+    metadata['markers'][name] = {
+        'clade': clade,
+        'ext': set(gca),
+        'len': m_length,
+        'taxon': taxon
+    }
+
+    dump_to_json(metadata, out_json_fp)
+
+
+def format_markers(marker_l):
+    '''
+    Format markers
+
+    :param marker_l: list of markers
+    '''
+    markers = []
+    for m in marker_l:
+        m = m.rstrip()
+        if ' ' in m:
+            markers.append(m.split(' ')[0])
+        else:
+            markers.append(m)
+    return markers
+
+
+def get_markers(marker_fp):
+    '''
+    Get markers from a file
+
+    :param marker_fp: Path to file with markers (1 per line)
+    '''
+    # load markers
+    with open(marker_fp, 'r') as marker_f:
+        markers = marker_f.readlines()
+
+    # format markers
+    markers = format_markers(markers)
+
+    return markers
+
+
+def check_not_found_markers(found_markers, original_markers):
+    '''
+    Check list of markers
+
+    :param found_markers: list of found markers
+    :param original_markers: list of original markers
+    '''
+    if len(found_markers) != len(original_markers):
+        print('markers not found:')
+        for m in original_markers:
+            if m not in found_markers:
+                print('- "%s"' % m)
+
+
+def prune_taxonomy(in_taxonomy, taxon_s, gca_s):
+    '''
+    Prune taxonomy to keep only listed taxonomy
+
+    :param in_taxonomy: dictionary with list of taxonomy
+    :param taxon_s: set of taxons to keep
+    :param gca_s: set of GCA ids to keep
+    '''
+    out_taxonomy = {}
+    kept_taxonomy = set()
+    kept_taxons = set()
+    kept_gca = set()
+    for t, v in in_taxonomy.items():
+        # check if t match element in list of taxon_s
+        kept_taxon = False
+        for t_k in taxon_s:
+            if t_k in t:
+                kept_taxon = True
+                out_taxonomy[t] = v
+                kept_taxonomy.add(t)
+                kept_taxons.add(t_k)
+                break
+        # check if GCA in the taxon id
+        s = re.search(r'GCA_\d+$', t)
+        if s:
+            gca = s[0]
+            # check if GCA in taxon id is in the list GCA to keep
+            if gca in gca_s:
+                kept_gca.add(gca)
+                if not kept_taxon:
+                    out_taxonomy[t] = v
+                    kept_taxonomy.add(t)
+
+    print('%s kept taxonomy' % len(kept_taxonomy))
+    print('%s / %s taxons not found' % (len(taxon_s) - len(kept_taxons), len(taxon_s)))
+    print('%s / %s GCA taxons not found' % (len(gca_s) - len(kept_gca), len(gca_s)))
+    return out_taxonomy
+
+
+def remove_markers(in_json_fp, marker_fp, out_json_fp, kept_marker_fp):
+    '''
+    Remove markers from JSON file
+
+    :param in_json_fp: Path to input JSON file
+    :param marker_fp: Path to file with markers to remove (1 per line)
+    :param out_json_fp: Path to output JSON file
+    :param kept_marker_fp: Path to file with kept markers
+    '''
+    in_metadata = load_from_json(in_json_fp)
+
+    # load markers
+    markers_to_remove = set(get_markers(marker_fp))
+    print('%s markers to remove' % len(markers_to_remove))
+
+    # keep merged_taxon
+    out_metadata = {
+        'markers': {},
+        'taxonomy': {},
+        'merged_taxon': in_metadata['merged_taxon']
+    }
+
+    # parse markers to keep
+    removed_markers = []
+    kept_markers = []
+    taxons_to_keep = set()
+    gca_to_keep = set()
+    for m, v in in_metadata['markers'].items():
+        if m not in markers_to_remove:
+            out_metadata['markers'][m] = v
+            kept_markers.append(m)
+            taxons_to_keep.add(v['taxon'])
+            gca_to_keep.update(v['ext'])
+        else:
+            removed_markers.append(m)
+    print('%s removed markers' % len(removed_markers))
+
+    # check markers that are not found
+    check_not_found_markers(removed_markers, markers_to_remove)
+
+    # keep only taxonomy in taxons_to_keep or with GCA in gca_to_keep
+    out_metadata['taxonomy'] = prune_taxonomy(in_metadata['taxonomy'], taxons_to_keep, gca_to_keep)
+
+    # save to JSON
+    dump_to_json(out_metadata, out_json_fp)
+
+    # write list of kept markers
+    with open(kept_marker_fp, 'w') as kept_marker_f:
+        for m in kept_markers:
+            kept_marker_f.write("%s\n" % m)
+
+
+def keep_markers(in_json_fp, marker_fp, out_json_fp):
+    '''
+    Keep markers from JSON file, others will be removed
+
+    :param in_json_fp: Path to input JSON file
+    :param marker_fp: Path to file with markers to keep (1 per line)
+    :param out_json_fp: Path to output JSON file
+    '''
+    in_metadata = load_from_json(in_json_fp)
+
+    # load markers
+    markers_to_keep = set(get_markers(marker_fp))
+    print('%s markers to keep' % len(markers_to_keep))
+
+    # keep merged_taxon
+    out_metadata = {
+        'markers': {},
+        'taxonomy': {},
+        'merged_taxon': in_metadata['merged_taxon']
+    }
+
+    # parse markers to keep
+    kept_markers = []
+    taxons_to_keep = set()
+    gca_to_keep = set()
+    for m, v in in_metadata['markers'].items():
+        if m in markers_to_keep:
+            out_metadata['markers'][m] = v
+            kept_markers.append(m)
+            taxons_to_keep.add(v['taxon'])
+            gca_to_keep.update(v['ext'])
+    print('%s kept markers' % len(kept_markers))
+
+    # check markers that are not found
+    check_not_found_markers(kept_markers, markers_to_keep)
+
+    # keep only taxonomy in taxons_to_keep or with GCA in gca_to_keep
+    out_metadata['taxonomy'] = prune_taxonomy(in_metadata['taxonomy'], taxons_to_keep, gca_to_keep)
+
+    # save to JSON
+    dump_to_json(out_metadata, out_json_fp)
+
+
+if __name__ == '__main__':
+    # Read command line
+    parser = argparse.ArgumentParser(description='Customize MetaPhlan database')
+    subparsers = parser.add_subparsers(dest='function')
+    # transform_pkl_to_json subcommand
+    pkl_to_json_parser = subparsers.add_parser('transform_pkl_to_json', help='Transform Pickle to JSON to get marker metadata')
+    pkl_to_json_parser.add_argument('--pkl', help="Path to input Pickle file")
+    pkl_to_json_parser.add_argument('--json', help="Path to output JSON file")
+    # transform_json_to_pkl subcommand
+    json_to_pkl_parser = subparsers.add_parser('transform_json_to_pkl', help='Transform JSON to Pickle to push marker metadata')
+    json_to_pkl_parser.add_argument('--json', help="Path to input JSON file")
+    json_to_pkl_parser.add_argument('--pkl', help="Path to output Pickle file")
+    # add_marker subcommand
+    add_marker_parser = subparsers.add_parser('add_marker', help='Add new marker to JSON file')
+    add_marker_parser.add_argument('--in_json', help="Path to input JSON file")
+    add_marker_parser.add_argument('--out_json', help="Path to output JSON file")
+    add_marker_parser.add_argument('--name', help="Name of new marker")
+    add_marker_parser.add_argument('--m_length', help="Length of new marker")
+    add_marker_parser.add_argument('--g_length', help="Length of genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--gca', help="GCA of genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--k_name', help="Name of Kingdom for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--k_id', help="NCBI id of Kingdom for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--p_name', help="Name of Phylum for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--p_id', help="NCBI id of Phylum for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--c_name', help="Name of Class for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--c_id', help="NCBI id of Class for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--o_name', help="Name of Order for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--o_id', help="NCBI id of Order for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--f_name', help="Name of Family for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--f_id', help="NCBI id of Family for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--g_name', help="Name of Genus for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--g_id', help="NCBI id of Genus for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--s_name', help="Name of Species for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--s_id', help="NCBI id of Species for genome from which the new marker has been extracted", action="append")
+    add_marker_parser.add_argument('--t_name', help="Name of Strain for genome from which the new marker has been extracted", action="append")
+    # remove_markers subcommand
+    remove_markers_parser = subparsers.add_parser('remove_markers', help='Remove markers from JSON file')
+    remove_markers_parser.add_argument('--in_json', help="Path to input JSON file")
+    remove_markers_parser.add_argument('--markers', help="Path to file with markers to remove (1 per line)")
+    remove_markers_parser.add_argument('--out_json', help="Path to output JSON file")
+    remove_markers_parser.add_argument('--kept_markers', help="Path to file with kept markers")
+    # keep_markers subcommand
+    keep_markers_parser = subparsers.add_parser('keep_markers', help='Keep markers from JSON file, others will be removed')
+    keep_markers_parser.add_argument('--in_json', help="Path to input JSON file")
+    keep_markers_parser.add_argument('--markers', help="Path to file with markers to keep (1 per line)")
+    keep_markers_parser.add_argument('--out_json', help="Path to output JSON file")
+
+    args = parser.parse_args()
+
+    if args.function == 'transform_pkl_to_json':
+        transform_pkl_to_json(Path(args.pkl), Path(args.json))
+    elif args.function == 'transform_json_to_pkl':
+        transform_json_to_pkl(Path(args.json), Path(args.pkl))
+    elif args.function == 'add_marker':
+        add_marker(
+            args.in_json,
+            args.out_json,
+            args.name,
+            args.m_length,
+            args.g_length,
+            args.gca,
+            args.k_name,
+            args.k_id,
+            args.p_name,
+            args.p_id,
+            args.c_name,
+            args.c_id,
+            args.o_name,
+            args.o_id,
+            args.f_name,
+            args.f_id,
+            args.g_name,
+            args.g_id,
+            args.s_name,
+            args.s_id,
+            args.t_name)
+    elif args.function == 'remove_markers':
+        remove_markers(args.in_json, args.markers, args.out_json, args.kept_markers)
+    elif args.function == 'keep_markers':
+        keep_markers(args.in_json, args.markers, args.out_json)

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/humann_barplot.xml	Wed May 12 09:00:09 2021 +0000
@@ -0,0 +1,295 @@
+<tool id="humann_barplot" name="Barplot" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
+    <description>stratified HUMAnN features</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="edam_ontology"/>
+    <expand macro="requirements"/>
+    <expand macro="version"/>
+    <command detect_errors="exit_code"><![CDATA[
+humann_barplot
+    --input '$input'
+#if str($last_metadata) != ''
+    --last-metadata '$last_metadata'
+#end if
+    --focal-feature '$focal_feature'
+    --top-taxa $species.top_taxa
+    $species.as_genera 
+    $species.exclude_unclassified
+    $species.remove_zeros
+    --sort $species.sort
+#if str($species.taxa_colormap) != ''
+    --taxa-colormap '$species.taxa_colormap'
+#end if
+
+#if str($sample.focal_metadata) != ''
+    --focal-metadata '$sample.focal_metadata'
+#end if
+#if str($sample.meta_colormap) != ''
+    --meta-colormap '$sample.meta_colormap'
+#end if
+    --max-metalevels $sample.max_metalevels
+    --scaling '$graphical.scaling'
+    #if str($graphical.ymin)  and str($graphical.ymax) != ''
+    --ylims $graphical.ymin $graphical.ymax
+#end if
+    $graphical.no_grid
+    --dimensions $graphical.height $graphical.width
+#if str($graphical.units) != ''
+    --units '$graphical.units'
+#end if
+    --legend-cols $legend.legend_cols
+    --legend-rows $legend.legend_rows
+    --legend-height $legend.legend_height
+#if $out.format == 'png'
+    --output 'output.png'
+#elif $out.format == 'pdf'
+    --output 'output.pdf'
+#elif $out.format == 'svg'
+    --output 'output.svg'
+#end if
+    ]]></command>
+    <inputs>
+        <param argument="--input" type="data" format="tsv,tabular" label="HUMAnN table with optional metadata"/>
+        <param argument="--last-metadata" type="text" value="" optional="true" label="The name (header) of the last row containing metadata, if any">
+            <sanitizer invalid_char="">
+                <valid initial="string.ascii_letters,string.digits">
+                    <add value="_" />
+                    <add value="-" />
+                </valid>
+            </sanitizer>
+        </param>
+        <param argument="--focal-feature" type="text" value="" label="Feature ID of interest" help="Give ID not full name">
+            <sanitizer invalid_char="">
+                <valid initial="string.ascii_letters,string.digits">
+                    <add value="_" />
+                    <add value="-" />
+                </valid>
+            </sanitizer>
+            <validator type="empty_field" />
+        </param>
+        <section name="species" title="Manipulation of species contributions" expanded="true">
+            <param argument="--top-taxa" type="integer" value="18" min="0" label="Max taxon stratifications"/>
+            <param argument="--as-genera" type="boolean" truevalue="--as-genera" falsevalue="" checked="false" label="Collapse species to genera?"/>
+            <param argument="--exclude-unclassified" type="boolean" truevalue="--exclude-unclassified" falsevalue="" checked="false" label="Do not include the 'unclassified' stratum?"/>
+            <param argument="--remove-zeros" type="boolean" truevalue="--remove-zeros" falsevalue="" checked="false" label="Do not plot samples with zero sum for this feature?"/>
+            <param argument="--sort" type="select" multiple="true" label="Sample sorting methods">
+                <option value="none">Default</option>
+                <option value="sum">Sum of stratified values</option>
+                <option value="dominant">Value of the most dominant stratification</option>
+                <option value="brawcurtis">Bray-Curtis agreement of relative stratifications</option>
+                <option value="braycurtis_w">Bray-Curtis agreement of raw stratifications</option>
+                <option value="metadata">Given metadata label</option>
+            </param>
+            <param argument="--taxa-colormap" type="text" value="" optional="true" label="Color space for taxa" help="Automatic if not provided">
+                <sanitizer invalid_char="">
+                    <valid initial="string.ascii_letters,string.digits">
+                        <add value="_" />
+                        <add value="-" />
+                    </valid>
+                </sanitizer>
+            </param>
+        </section>
+        <section name="sample" title="Sample metadata" expanded="true">
+            <param argument="--focal-metadata" type="text" value="" optional="true" label="Metadata to highlight">
+                <sanitizer invalid_char="">
+                    <valid initial="string.ascii_letters,string.digits">
+                        <add value="_" />
+                        <add value="-" />
+                    </valid>
+                </sanitizer>
+            </param>
+            <param argument="--max-metalevels" type="integer" value="7" min="0" label="Number of most frequent metadata to keep" help="Others will be collapsed"/>
+            <param argument="--meta-colormap" type="text" value="" optional="true" label="Color space for metadata levels" help="Automatic if not provided">
+                <sanitizer invalid_char="">
+                    <valid initial="string.ascii_letters,string.digits">
+                        <add value="_" />
+                        <add value="-" />
+                    </valid>
+                </sanitizer>
+            </param>
+        </section>
+        <section name="graphical" title="Graphical tweaks" expanded="true">
+            <param argument="--scaling" type="select" optional="true" label="Scaling options for total bar heights" help="Taxa are always proportional to height">
+                <option value="original">Plot original units</option>
+                <option value="logstack">Community totals (stacked bar peaks) are log10-scaled</option>
+                <option value="totalsum">Community totals (stacked bar peaks) are fixed at 1.0</option>
+            </param>
+            <param name="ymin" type="float" value="" optional="true" label="Minimum for y-axis"/>
+            <param name="ymax" type="float" value="" optional="true" label="Minimum for y-axis"/>
+            <param argument="--no-grid" type="boolean" truevalue="--no-grid" falsevalue="" checked="true" label="Don't plot y-axis grid lines?"/>
+            <param name="height" argument="--dimensions" type="float" value="11" label="Image height in inches"/>
+            <param name="width" argument="--dimensions" type="float" value="6" label="Image width in inches"/>
+            <param argument="--units" type="text" value="" label="Name of y-axis abundance units" help="Automatic if not provided">
+                <sanitizer invalid_char="">
+                    <valid initial="string.ascii_letters,string.digits">
+                        <add value="_" />
+                    </valid>
+                </sanitizer>
+            </param>
+        </section>
+        <section name="legend" title="Legend layout" expanded="true">
+            <param argument="--legend-cols" type="integer" value="3" min="0" label="Number of legend columns"/>
+            <param argument="--legend-rows" type="integer" value="10" min="0" label="Number of legend rows"/>
+            <param argument="--legend-height" type="float" value="1.0" min="0" label="Ratio of legend to data axis height"/>
+        </section>
+        <section name="out" title="Outputs" expanded="true">
+            <param name="format" type="select" label="Format">
+                <option value="pdf">PDF</option>
+                <option value="png">PNG</option>
+                <option value="svg">SVG</option>
+            </param>
+        </section>
+    </inputs>
+    <outputs>
+        <data name="barplot_png" format="png" from_work_dir="output.png">
+            <filter>out['format'] == 'png'</filter>
+        </data>
+        <data name="barplot_pdf" format="pdf" from_work_dir="output.pdf">
+            <filter>out['format'] == 'pdf'</filter>
+        </data>
+        <data name="barplot_svg" format="svg" from_work_dir="output.svg">
+            <filter>out['format'] == 'svg'</filter>
+        </data>
+    </outputs>
+    <tests>
+        <test expect_num_outputs="1">
+            <param name="input" value="hmp_pathabund.txt"/>
+            <param name="last_metadata" value="STSite"/>
+            <param name="focal_feature" value="ANAGLYCOLYSIS-PWY"/>
+            <section name="species">
+                <param name="top_taxa" value="18"/>
+                <param name="as_genera" value="false"/>
+                <param name="exclude_unclassified" value="false"/>
+                <param name="remove_zeros" value="false"/>
+                <param name="sort" value="none"/>
+            </section>
+            <section name="sample">
+                <param name="focal_metadata" value="STSite"/>
+                <param name="max_metalevels" value="7"/>
+            </section>
+            <section name="graphical">
+                <param name="scaling" value="original"/>
+                <param name="no_grid" value="true"/>
+                <param name="height" value="8"/>
+                <param name="width" value="4"/>
+            </section>
+            <section name="legend">
+                <param name="legend_cols" value="3"/>
+                <param name="legend_rows" value="10"/>
+                <param name="legend_height" value="1.0"/>
+            </section>
+            <section name="out">
+                <param name="format" value="png"/>
+            </section>
+            <output name="barplot_png" file="barplot1.png" ftype="png" compare="sim_size"/>
+        </test>
+        <test expect_num_outputs="1">
+            <param name="input" value="hmp_pathabund.txt"/>
+            <param name="last_metadata" value="STSite"/>
+            <param name="focal_feature" value="ANAGLYCOLYSIS-PWY"/>
+            <section name="species">
+                <param name="top_taxa" value="18"/>
+                <param name="as_genera" value="false"/>
+                <param name="exclude_unclassified" value="false"/>
+                <param name="remove_zeros" value="false"/>
+                <param name="sort" value="sum"/>
+            </section>
+            <section name="sample">
+                <param name="focal_metadata" value="STSite"/>
+                <param name="max_metalevels" value="7"/>
+            </section>
+            <section name="graphical">
+                <param name="scaling" value="original"/>
+                <param name="no_grid" value="true"/>
+                <param name="height" value="8"/>
+                <param name="width" value="4"/>
+            </section>
+            <section name="legend">
+                <param name="legend_cols" value="3"/>
+                <param name="legend_rows" value="10"/>
+                <param name="legend_height" value="1.0"/>
+            </section>
+            <section name="out">
+                <param name="format" value="pdf"/>
+            </section>
+            <output name="barplot_svg" file="barplot2.pdf" ftype="pdf" compare="sim_size"/>
+        </test>
+        <test expect_num_outputs="1">
+            <param name="input" value="hmp_pathabund.txt"/>
+            <param name="last_metadata" value="STSite"/>
+            <param name="focal_feature" value="ANAGLYCOLYSIS-PWY"/>
+            <section name="species">
+                <param name="top_taxa" value="18"/>
+                <param name="as_genera" value="true"/>
+                <param name="exclude_unclassified" value="true"/>
+                <param name="remove_zeros" value="true"/>
+                <param name="sort" value="sum"/>
+            </section>
+            <section name="sample">
+                <param name="focal_metadata" value="STSite"/>
+                <param name="max_metalevels" value="7"/>
+            </section>
+            <section name="graphical">
+                <param name="scaling" value="original"/>
+                <param name="no_grid" value="false"/>
+                <param name="height" value="8"/>
+                <param name="width" value="4"/>
+            </section>
+            <section name="legend">
+                <param name="legend_cols" value="3"/>
+                <param name="legend_rows" value="10"/>
+                <param name="legend_height" value="1.0"/>
+            </section>
+            <section name="out">
+                <param name="format" value="svg"/>
+                <param argument="write_taxa_colors" value="false"/>
+                <param argument="write_sample_order" value="false"/>
+            </section>
+            <output name="barplot_svg" file="barplot3.svg" ftype="svg" compare="sim_size"/>
+        </test>
+        <test expect_num_outputs="1">
+            <param name="input" value="hmp_pathabund.txt"/>
+            <param name="last_metadata" value="STSite"/>
+            <param name="focal_feature" value="ANAGLYCOLYSIS-PWY"/>
+            <section name="species">
+                <param name="top_taxa" value="12"/>
+                <param name="as_genera" value="false"/>
+                <param name="exclude_unclassified" value="false"/>
+                <param name="remove_zeros" value="false"/>
+                <param name="sort" value="sum"/>
+            </section>
+            <section name="sample">
+                <param name="focal_metadata" value="STSite"/>
+                <param name="max_metalevels" value="7"/>
+            </section>
+            <section name="graphical">
+                <param name="scaling" value="original"/>
+                <param name="no_grid" value="true"/>
+                <param name="height" value="8"/>
+                <param name="width" value="4"/>
+            </section>
+            <section name="legend">
+                <param name="legend_cols" value="3"/>
+                <param name="legend_rows" value="10"/>
+                <param name="legend_height" value="1.0"/>
+            </section>
+            <section name="out">
+                <param name="format" value="png"/>
+            </section>
+            <output name="barplot_png" file="barplot4.png" ftype="png" compare="sim_size"/>
+        </test>
+    </tests>
+    <help><![CDATA[
+@HELP_HEADER@
+
+This tool produces plots of stratified HUMAnN features and includes many options for sorting and scaling data
+
+Here is an example of a HUMAnN barplot for a pathway (denitrification) that was preferentially enriched in Human Microbiome Project oral samples relative to other body sites.
+This figure uses many options from humann_barplot, including regrouping by genus, pseudolog scaling, and sorting samples by similarity and metadata:
+
+.. image:: $PATH_TO_IMAGES/731303924-page_DENITRIFICATION-PWY.png
+   :width: 800
+    ]]></help>
+    <expand macro="citations"/>
+</tool>

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/humann_genefamilies_genus_level	Wed May 12 09:00:09 2021 +0000
@@ -0,0 +1,52 @@
+<!-- The tool is broken with current version of HUMAnN. Once it will be fixed (PR merged), we can update this wrapper and add the XML extension to enable it -->
+<tool id="humann_genefamilies_genus_level" name="Create a genus level" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
+    <description>gene families and pathways from HUMAnN species level gene families</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="edam_ontology"/>
+    <expand macro="requirements"/>
+    <expand macro="version"/>
+    <command detect_errors="exit_code"><![CDATA[
+humann_genefamilies_genus_level 
+    --input '$input'
+    --output '$gene_families'
+&&
+humann
+    --input '$gene_families'
+    --output '$pathways'
+    ]]></command>
+    <inputs>
+        <param argument="--input" type="data" format="tsv,tabular" label="Species level gene families" help="Direct output from HUMAnN"/>
+    </inputs>
+    <outputs>
+        <data name="gene_families" format="tabular" label="${tool.name} on ${on_string}: Genus level gene families"/>
+        <data name="pathways" format="tabular" label="${tool.name} on ${on_string}: Genus level pathways"/>
+    </outputs>
+    <tests>
+        <test expect_num_outputs="1">
+            <param name="input" value="demo_genefamilies.tsv"/>
+            <output name="gene_families" ftype="tabular" value="genus-level-gene-families.tsv" compare="sim_size">
+                <assert_contents>
+                    <has_text text="humann_Abundance"/>
+                    <has_text text="PWY-5423: oleoresin monoterpene volatiles biosynthesis|unclassified"/>
+                    <has_n_columns n="2"/>
+                </assert_contents>
+            </output>
+            <output name="pathways" ftype="tabular" value="genus-level-pathways.tsv" compare="sim_size">
+                <assert_contents>
+                    <has_text text="humann_Abundance"/>
+                    <has_text text="PWY-5423: oleoresin monoterpene volatiles biosynthesis|unclassified"/>
+                    <has_n_columns n="2"/>
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    <help><![CDATA[
+@HELP_HEADER@
+
+By default, the gene families and pathways output files from HUMAnN are species level.
+This tool generates genus level gene families and pathways
+    ]]></help>
+    <expand macro="citations"/>
+</tool>

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/humann_infer_taxonomy	Wed May 12 09:00:09 2021 +0000
@@ -0,0 +1,81 @@
+<!-- The tool is broken with current version of HUMAnN. Once it will be fixed, we can update this wrapper and add the XML extension to enable it -->
+<tool id="humann_infer_taxonomy" name="Infex taxonomy" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
+    <description>for "unclassified" taxonomy in HUMAnN generated gene families</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="edam_ontology"/>
+    <expand macro="requirements"/>
+    <expand macro="version"/>
+    <command detect_errors="exit_code"><![CDATA[
+humann_infer_taxonomy
+    --input '$input'
+    --output '$output'
+    --level '$level'
+    --database '$database.fields.path'
+    --mode '$mode'
+    --lca-choice '$lca_choice'
+    --threshold $threshold
+    ]]></command>
+    <inputs>
+        <param argument="--input" type="data" format="tsv,tabular" label="Gene family table"/>
+        <param argument="--level" type="select" label="Desired level for taxonomic estimation/summation">
+            <option value="Kingdom">Kingdom</option>
+            <option value="Phylum">Phylum</option>
+            <option value="Class">Class</option>
+            <option value="Order">Order</option>
+            <option value="Family" selected="true">Family</option>
+            <option value="Genus">Genus</option>
+        </param>
+        <param argument="--database" type="select" label="UniRef-specific taxonomy database">
+            <options from_data_table="humann_utility_mapping">
+                <validator message="No utility mapping is available" type="no_options" />
+                <filter type="regexp" column="2" value="tol-lca" />
+            </options>
+        </param>
+        <param argument="--mode" type="select" label="Rows to include in the estimation/summation">
+            <option value="c_tmode" selected="true">Totals</option>
+            <option value="c_umode">Unclassified</option>
+            <option value="c_smode">Stratified</option>
+        </param>
+        <param argument="--lca-choice" type="select" label="Per-gene taxonomic annotation to consider">
+            <option value="source_tax">Source taxonomy</option>
+            <option value="uniref_lca">UniRef lowest common ancestor (LCA)</option>
+            <option value="humann_lca" selected="true">HUMAnN lowest common ancestor (LCA)</option>
+        </param>
+        <param argument="--threshold" type="float" value="1e-3" label="Minimum frequency for a new taxon to be included"/>
+    </inputs>
+    <outputs>
+        <data format="tabular" name="output"/>
+    </outputs>
+    <tests>
+        <test expect_num_outputs="1">
+            <param name="input" value=""/>
+            <param name="level" value="Kingdom"/>
+            <param name="database" value=""/>
+            <param name="mode" value="c_tmode"/>
+            <param name="lca_choice" value="source_tax"/>
+            <param name="threshold" value="1e-3"/>
+            <output name="output" ftype="tabular">
+                <assert_contents>
+                    <has_text text=""/>
+                    <has_line line=""/>
+                    <has_line_matching expression=""/>
+                    <has_n_columns n=""/>
+                    <has_size value="" delta=""/>
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    <help><![CDATA[
+@HELP_HEADER@
+
+Based on the lowest common ancestor (LCA) annotation
+of each UniRef50/90 cluster, this tool infers approximate taxonomy 
+for unclassified features at a target level of resolution.
+
+It will modify features of known genus/species to match 
+target level.
+    ]]></help>
+    <expand macro="citations"/>
+</tool>

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml	Wed May 12 09:00:09 2021 +0000
@@ -0,0 +1,38 @@
+<?xml version="1.0"?>
+<macros>
+    <token name="@TOOL_VERSION@">3.0.0</token>
+    <token name="@VERSION_SUFFIX@">0</token>
+    <token name="@PROFILE@">20.01</token>
+    <xml name="edam_ontology">
+        <edam_topics>
+            <edam_topic>topic_3174</edam_topic>
+            <edam_topic>topic_0194</edam_topic>
+        </edam_topics>
+        <edam_operations>
+            <edam_operation>operation_2478</edam_operation>
+            <edam_operation>operation_0324</edam_operation>
+        </edam_operations>
+    </xml>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="@TOOL_VERSION@">humann</requirement>
+            <yield/>
+        </requirements>
+    </xml>
+    <xml name="version">
+        <version_command>humann --version</version_command>
+    </xml>
+    <token name="@HELP_HEADER@">
+What it does
+============
+
+HUMAnN is a pipeline for efficiently and accuretly profiling the presence/absence and abundance of microbial pathways in a community from metagenomic or metatranscriptomic sequencing data. 
+
+Read more about the tool: http://huttenhower.sph.harvard.edu/humann
+    </token>
+    <xml name="citations">
+        <citations>
+            <citation type="doi">10.1371/journal.pcbi.1002358</citation>
+        </citations>
+    </xml>
+</macros>

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+    <dc:format>image/svg+xml</dc:format>
+    <dc:creator>
+     <cc:Agent>
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+    </dc:creator>
+   </cc:Work>
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