Mercurial > repos > iuc > hyphy_relax
diff hyphy_relax.xml @ 5:7827dfb71693 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hyphy/ commit 8d5ae1d04c43988fdcc458f4f08376a15e72db8e"
author | iuc |
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date | Thu, 20 Feb 2020 18:16:06 -0500 |
parents | 134d46bd47dd |
children | ea64cc9a06e4 |
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--- a/hyphy_relax.xml Mon Feb 17 14:52:33 2020 -0500 +++ b/hyphy_relax.xml Thu Feb 20 18:16:06 2020 -0500 @@ -24,11 +24,11 @@ <inputs> <expand macro="inputs"/> <expand macro="gencode"/> - <param name="analysisType" type="select" label="Analysis Type"> + <param argument="--model" name="analysisType" type="select" label="Analysis Type" > <option value="All">All</option> <option value="Minimal">Minimal</option> </param> - <param name="treeAnnotations" type="select" label="Tree Annotations"> + <param name="treeAnnotations" type="select" label="Tree Annotations" help = "Is there one or two sets of annotated branches in the Newick file?"> <option value="1">TEST only</option> <option value="2">TEST and REFERENCE</option> </param> @@ -45,11 +45,66 @@ </test> </tests> <help><![CDATA[ -RELAX is a hypothesis testing framework that asks whether the strength of natural selection has been relaxed or intensified along a specified set of test branches. RELAX is therefore not a suitable method for explicitly testing for positive selection. Instead, RELAX is most useful for identifying trends and/or shifts in the stringency of natural selection on a given gene. +RELAX: a test for selection differences +======================================= + +What question does this method answer? +-------------------------------------- + +Is there ev- idence the strength of selection has been relaxed (or conversely intensified) on a specified group of lineages (TEST) +relative to a set of reference lineages (RELAX)? Importantly, RELAX is not designed to detect diversifying selection specifically. +We note that the RELAX framework can perform both this specific hypothesis test as well as fit a suite of descriptive models which address, +or example, overall rate differences between test and reference branches or lineage-specific inferences of selection relaxation. + +Recommended Applications +------------------------ + +1. Testing for a systematic shift (relaxation / intensification) in the distribution of selection pressure associated with major biological transitions such as hosting switching in viruses, lifestyle evolution in bacteria (i.e. transition from free-living to endosymbiotic lifestyle +2. Comparing selective regimes between two subsets of branches in the tree, e.g. to investigate selective differences due an environmental/phenotypic changes. + + +Brief description +----------------- + +RELAX (a random effects test of selection relaxation) uses a random +effects branch-site model framework to test whether a set of 'Test' +branches evolves under relaxed selection relative to a set of +'Reference' branches (R), as measured by the relaxation parameter (K). + +Input +----- -See the online documentation_ for more information. + 1. A *FASTA* sequence alignment. + 2. A phylogenetic tree in the *Newick* format that is further partitioned into one or two groups (_TEST_ or _TEST_ and _REFERENCE_). See http://hyphy.org/tutorials/phylotree/ + +Note: the names of sequences in the alignment must match the names of the sequences in the tree. + + +Output +------ + +A JSON file with analysis results (http://hyphy.org/resources/json-fields.pdf). + +A custom visualization module for viewing these results is available (see http://vision.hyphy.org/RELAX for an example) + +Further reading +--------------- -.. _documentation: http://hyphy.org/methods/selection-methods/#relax +http://hyphy.org/methods/selection-methods/#RELAX + + +Tool options +------------ +:: + + + --code Which genetic code to use + + --model Which type of test to run + All : fit 4 different models [default] + Minimal: only perform a 2-model test of relaxation/intensification [faster] + + ]]></help> <expand macro="citations"> <citation type="doi">10.1093/molbev/msu400</citation>