# HG changeset patch # User iuc # Date 1641293562 0 # Node ID 7c77b2896ac2eae78dd378abf18ecb5cd033101c "planemo upload for repository https://forgemia.inra.fr/ices_imes_analysis/icescreen commit e77a58fd03abe136aca7b79db16be4644520bb64" diff -r 000000000000 -r 7c77b2896ac2 icescreen.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/icescreen.xml Tue Jan 04 10:52:42 2022 +0000 @@ -0,0 +1,202 @@ + + detects and annotates ICEs (Integrative and Conjugative Elements) and IMEs (Integrative and Mobilizable Elements) in Firmicutes genomes. + + 1.0.1 + 0 + + + icescreen + zip + + + + ./tmp_icescreen/ICEscreen_log.txt 2>&1 + + && cat ./tmp_icescreen/ICEscreen_results/results/${gbname}/icescreen_detection_ME/${gbname}_detected_ME.summary > '${summary}' + && cat ./tmp_icescreen/ICEscreen_results/results/${gbname}/icescreen_detection_ME/${gbname}_detected_SP_withMEIds.tsv > '${detected_sp}' + && cat ./tmp_icescreen/ICEscreen_results/results/${gbname}/icescreen_detection_ME/${gbname}_detected_ME.tsv > '${detected_me}' + + #if "output_annotation_genbank" in $additional_output.optional_files: + && mv ./tmp_icescreen/ICEscreen_results/results/${gbname}/visualization_files/${gbname}_icescreen.gb '${gbout}' + #end if + #if "output_annotation_gff" in $additional_output.optional_files: + && mv ./tmp_icescreen/ICEscreen_results/results/${gbname}/visualization_files/${gbname}_icescreen.gff '${gffout}' + #end if + #if "output_annotation_embl" in $additional_output.optional_files: + && mv ./tmp_icescreen/ICEscreen_results/results/${gbname}/visualization_files/${gbname}_icescreen.embl '${emblout}' + #end if + #if "output_log" in $additional_output.optional_files: + && mv ./tmp_icescreen/ICEscreen_log.txt '${log}' + #end if + #if "output_zip_all_files" in $additional_output.optional_files: + && cd ./tmp_icescreen/ICEscreen_results/results/${gbname} && zip --quiet -r - -- . > '${outzip}' + #end if + ]]> + + + +
+ + + + +
+
+ + + + + + + +
+
+ + + + + + additional_output['optional_files'] and "output_annotation_genbank" in additional_output['optional_files'] + + + additional_output['optional_files'] and "output_annotation_gff" in additional_output['optional_files'] + + + additional_output['optional_files'] and "output_annotation_embl" in additional_output['optional_files'] + + + additional_output['optional_files'] and "output_zip_all_files" in additional_output['optional_files'] + + + additional_output['optional_files'] and "output_log" in additional_output['optional_files'] + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + @UNPUBLISHED{Kim07aninterior-point, + author = {Julie Lao and Thomas Lacroix and Gérard Guédon and Charles Coluzzi and Nathalie Leblond-Bourget and Hélène Chiapello}, + title = {"See our latest publication at https://icescreen.migale.inrae.fr."} + } + + +
diff -r 000000000000 -r 7c77b2896ac2 test-data/NC_004668_137848_164286_detected_ME.summary --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/NC_004668_137848_164286_detected_ME.summary Tue Jan 04 10:52:42 2022 +0000 @@ -0,0 +1,44 @@ +##################### Mobile elements detection statistics ##################### +# Number of complete elements +Complete ICE (4 types of SP): 1 +Complete IME (R+I, R+C+I): 0 + +# Number of complete modules +Conjugation module (R+C+V): 0 +Mobilizable element (R+C with size <= 10 CDS): 0 + +# Number of partial elements +Partial ICE (at least V): 0 +Other partial element (R+C, R+V, V+C with size > 10 CDS): 0 + +# Composite elements +Total nested elements (partial or complete): 0 +Host element: 0 +Guest element: 0 +Element that are both host and guest: 0 + +################### Signature proteins detection statistics #################### +# Total SP detected +Total SP: 4 +Total Integrase: 1 +Total Relaxase: 1 +Total Coupling protein: 1 +Total VirB4: 1 + +# Unassigned SP +Unassigned SP: 0 +Unassigned Integrase: 0 +Unassigned Relaxase: 0 +Unassigned Coupling protein: 0 +Unassigned VirB4: 0 + +############################# Segments statistics ############################## +Number of segments: 1 +Segment with one element: 1 +Segment with several elements: 0 +Segment with nested elements: 0 +Segment with no element: 0 + +############################### Parameters used ################################ +Maximum CDS numbers in initial segments: 100 +Maximum CDS numbers in an IME element: 10 diff -r 000000000000 -r 7c77b2896ac2 test-data/NC_004668_137848_164286_detected_ME.tsv --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/NC_004668_137848_164286_detected_ME.tsv Tue Jan 04 10:52:42 2022 +0000 @@ -0,0 +1,2 @@ +ICE IME Number segment number category of element category of integrase host ICE / IME guest ICE / IME other ICE / IME colocalized ICEline format ICE SuperFamily From Blast Of SP Conj Module ICE Family From Blast Of SP Conj Module IME Family From Blast Of SP Conj Module family SP conj module HMM integrase upstream integrase downstream relaxase coupling protein VirB4 list ordered SPs SPs conj module to manually review Integrase to manually review comment +2 1 Complete ICE (4 types of SP) one integrase - Tyr C3.R1:V7.I Tn916 ICESt3 C:TcpA, R:MOBT, V:VirB4 WP_002392915.1-25273 WP_002359299.1-3098 WP_002359295.1-1 WP_002359320.1-17920 WP_002359295.1-1, WP_002359299.1-3098, WP_002359320.1-17920, WP_002392915.1-25273 Integrase WP_002392915.1-25273 has been associated to the structure 2 because they are adjacent, this integrase has already be seen rattached to this conjugaison module family, and there is no upstream/downstream ambiguity. diff -r 000000000000 -r 7c77b2896ac2 test-data/NC_004668_137848_164286_icescreen.embl --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/NC_004668_137848_164286_icescreen.embl Tue Jan 04 10:52:42 2022 +0000 @@ -0,0 +1,72 @@ +ID NC_004668; SV 1; ; DNA; ; UNC; 0 BP. +XX +AC NC_004668; +XX +PR Project:PRJNA224116; +XX +DE Enterococcus faecalis V583, complete sequence +XX +OS . +OC . +XX +FH Key Location/Qualifiers +FH +FT CDS 1..1511 +FT /origid="WP_002359295.1" +FT /function="Coupling Protein" +FT /protein_id="WP_002359295.1" +FT /locus_tag="EF_RS00630" +FT /codon_start=1 +FT /color=6 +FT /note="ICEscreen prediction: Coupling protein; BlastP +FT result (Annotation confidence: HIGH): Coupling protein TcpA +FT [Hit with AAO80014; Identity:100.00%; E-value:0; Query +FT coverage:100.00%]; Hmmscan result: TcpA [Hit with tcpA HMM +FT profile; E-value:0; i-Evalue:0]" +FT CDS 3098..4380 +FT /origid="WP_002359299.1" +FT /function="Relaxase" +FT /protein_id="WP_002359299.1" +FT /locus_tag="EF_RS00650" +FT /codon_start=1 +FT /color=7 +FT /note="ICEscreen prediction: Relaxase; BlastP result +FT (Annotation confidence: HIGH): Relaxase MOBT (PF02486) [Hit +FT with AAO80018; Identity:99.77%; E-value:0; Query +FT coverage:95.53%]; Hmmscan result: MOBT [Hit with T4SS_MOBT +FT HMM profile; E-value:0; i-Evalue:0]" +FT CDS 17920..20426 +FT /origid="WP_002359320.1" +FT /function="VirB4" +FT /protein_id="WP_002359320.1" +FT /locus_tag="EF_RS00720" +FT /codon_start=1 +FT /color="184 134 11" +FT /note="ICEscreen prediction: VirB4; BlastP result +FT (Annotation confidence: HIGH): VirB4 [Hit with CAE52368; +FT Identity:41.15%; E-value:0; Query coverage:99.76%]; Hmmscan +FT result: VirB4 [Hit with T4SS_virb4 HMM profile; E-value:0; +FT i-Evalue:0]" +FT CDS 25273..26438 +FT /origid="WP_002392915.1" +FT /function="Tyrosine Integrase" +FT /protein_id="WP_002392915.1" +FT /locus_tag="EF_RS00760" +FT /codon_start=1 +FT /color=8 +FT /note="ICEscreen prediction: Tyrosine integrase; BlastP +FT result (Annotation confidence: HIGH): Tyrosine integrase +FT [Hit with AAO80040; Identity:100.00%; E-value:0; Query +FT coverage:100.00%]; Hmmscan result: Tyrosine integrase [Hit +FT with Phage_integrase HMM profile; E-value:0; i-Evalue:0]" +FT mobile_element 1..26439 +FT /mobile_element_type="other: integrative and conjugative +FT element" +FT /note="ICEscreen prediction: Putative ICE (one integrase - +FT Tyr) [Element structure: Single; ICE superfamily: Tn916; +FT ICE family: ICESt3; Relaxase family: MOBT; Coupling protein +FT family: TcpA] (ICEscreen ID: 2)" +FT /color=15 +XX +SQ Sequence 0 BP; 0 A; 0 C; 0 G; 0 T; 0 other; +// diff -r 000000000000 -r 7c77b2896ac2 test-data/NC_004668_137848_164286_icescreen.gb --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/NC_004668_137848_164286_icescreen.gb Tue Jan 04 10:52:42 2022 +0000 @@ -0,0 +1,1108 @@ +LOCUS NC_004668 26439 bp DNA UNK 01-JAN-1980 +DEFINITION Enterococcus faecalis V583, complete sequence. +ACCESSION NC_004668 +VERSION NC_004668.1 +DBLINK BioProject: PRJNA224116 + BioSample: SAMN02603978 + Assembly: GCF_000007785.1 +KEYWORDS . +SOURCE . + ORGANISM . + . +FEATURES Location/Qualifiers + source 1..26439 + /organism="Enterococcus faecalis V583" + /mol_type="genomic DNA" + /strain="V583" + /db_xref="taxon:226185" + gene 1..1512 + /locus_tag="EF_RS00630" + /old_locus_tag="EF0139" + /old_locus_tag="EF_0139" + CDS 1..1512 + /locus_tag="EF_RS00630" + /old_locus_tag="EF0139" + /old_locus_tag="EF_0139" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359295.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="cell division protein FtsK" + /protein_id="WP_002359295.1" + /translation="MLKKLFRYRGRRIRYSSRNLLALYRVLFFMPILACLGYFVGYKWI + YPLYLSNPPDWKIYIVPALIIVGISIGAIVLITLLIKASIINSGYFSKVEQRQVLAHMI + IDNGYYTKKQVKSSDGKTKEKIKFPKIYYKSAKNSIFVSFETAGNKFQEKFETIGGFLE + TTFHADNLNKIDEKGFVTYELATDVYNKRIWIKDMQADEGKVQLMKGLYWHFDKDPHLL + LGGGTGGGKTFTILSLIYALCRVGEVEICDPKNSDLMALGKLPLFAGKVHTGKKDITQC + LENTVELMETRFKTMNNSSRYKMGKNYAYYGLKPKFVFIDEFAAFKAELANDYSTDGEV + DEYLTQLILKARQAGIFFIVAMQRPDGEFLKTALRDQFMFRMSVGRLSETGILMIFGDE + NKNKKFKYVEKIDGQKVYGRGYVAQGGGTAREFYSPQVPQDFDFIEEFIKISKELGYED + VPKEVQEEVSQKISKHIDKEALAEIDEEFQAEKNQLSELSEKYSA" + gene 1552..2085 + /locus_tag="EF_RS00635" + /old_locus_tag="EF0140" + /old_locus_tag="EF_0140" + CDS 1552..2085 + /locus_tag="EF_RS00635" + /old_locus_tag="EF0140" + /old_locus_tag="EF_0140" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359296.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359296.1" + /translation="MDSNEIIKRVRERVYREVKKKYTRDDLDTRIQDVLYYRSETYMKL + VSFANGKRIKKLADPRKFEKFMDTKGVKIVAEVLDGLNNQPKMQAMEYEQKVLTKVRQW + YQKKNHPELVDLEEEAFEQLVEKNIIYKKMKKRLYEEQDNQGFVYSDNFDMQLIRDSCD + IEEALYLDITLGDY" + gene 2106..2393 + /locus_tag="EF_RS00640" + /old_locus_tag="EF0141" + /old_locus_tag="EF_0141" + CDS 2106..2393 + /locus_tag="EF_RS00640" + /old_locus_tag="EF0141" + /old_locus_tag="EF_0141" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359297.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359297.1" + /translation="MKFKSRVQMLNYVIGKKPDIMKTVDRRAEKEGVTDDNRLLDYKLL + VISVLDAEAVYDEDRPIEEKKFSTSQEVDKYLETRFKEHMDSAWKEMFNK" + gene 2403..2807 + /locus_tag="EF_RS00645" + /old_locus_tag="EF0142" + /old_locus_tag="EF_0142" + CDS 2403..2807 + /locus_tag="EF_RS00645" + /old_locus_tag="EF0142" + /old_locus_tag="EF_0142" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002414864.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002414864.1" + /translation="MYSFDQVILELDNAIALKTLKNWANRIEKMTDTRFERRYAKNSAG + HSYSYKVFSIKDIENFQELLRLRENNVPLNEAINEVFMSDENKATHINKQEIEDLKRDM + RQLLKVSQSILAENASLKERIGALERGTAE" + gene 3098..4381 + /locus_tag="EF_RS00650" + /old_locus_tag="EF0143" + /old_locus_tag="EF_0143" + CDS 3098..4381 + /locus_tag="EF_RS00650" + /old_locus_tag="EF0143" + /old_locus_tag="EF_0143" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_016627242.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="replication initiation factor domain-containing + protein" + /protein_id="WP_002359299.1" + /translation="MRGVDLKRYRKELKLKQQELASKLGIERSLISKIESGKRVISKEL + EQKIINVLNLDGGHASVEAKIDFLRIRFKTLDVRTVIEKLLHMDMNWFTHESRGFYHYT + ETFSYSSIRIFRNPENVNMGIMLDLSGEGCRQLEEIFEEDNNRSWTEFFRSLYDDDIFG + QGILVDTKITRIDIALDELIVKGQENFDLYVLKEKMEQGLVDTTFKNFDFSGGFVYENK + KMVNKGLSLYFGSRQSPLYFNFYQKDYELARKESMSVEEAREKHEIKNRYEIRLSDEKA + FLFVEYFLSSGESLDWLVKEIINQSLTVYDIEDDMKVYCKSWHDVVDKLEGLKLSVQGE + KPSIEKTLRWLSNYLAPSLKMIKEIDNLLGTNELMERIDLAELKEKHEEIIEMVSVDAK + DLLFTNRDNKSVRSYMEREFELEEMYPF" + gene 4399..5013 + /locus_tag="EF_RS00655" + /old_locus_tag="EF0144" + /old_locus_tag="EF_0144" + CDS 4399..5013 + /locus_tag="EF_RS00655" + /old_locus_tag="EF0144" + /old_locus_tag="EF_0144" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002393276.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002393276.1" + /translation="MDKELIKKNIKLMDATIEKMIHVMSSIHKLQLSVKELEDSNELTV + SYVKRECTKLEERVKELRPSISRCVFMYREYVEEFETKLRDRIVTEKFFRIKRFYGKEV + LAFKEFQEKYQNYIPKDIIDIKKQVRQKLEEVGYEIDGAFEGDFTSWVGVYARPKDKPT + YLDPANAEEVVLQEKYSVNGFKQDFSEWFEFEIKDNVVYGI" + gene 5111..5416 + /locus_tag="EF_RS00660" + /old_locus_tag="EF0145" + /old_locus_tag="EF_0145" + CDS 5111..5416 + /locus_tag="EF_RS00660" + /old_locus_tag="EF0145" + /old_locus_tag="EF_0145" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002365956.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_010782977.1" + /translation="MTKKLIKIWRKEMLALMNEDADWYRNEDTERFKRIQELARKIETA + NTRQFSSHISKERFEAYQKMGLQFKEIAEEFHITTTALQQWRKDNGYPIYNKNNRK" + gene 5427..8132 + /gene="sea1" + /locus_tag="EF_RS00665" + /old_locus_tag="EF0146" + /old_locus_tag="EF_0146" + /gene_synonym="prgA" + CDS 5427..8132 + /gene="sea1" + /locus_tag="EF_RS00665" + /old_locus_tag="EF0146" + /old_locus_tag="EF_0146" + /gene_synonym="prgA" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_011109619.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="surface exclusion protein SEA1/PrgA" + /protein_id="WP_010784266.1" + /translation="MKKIASAGLSILVATGVAGIGGNEVQAAEQAQPKTPENSSTEQPA + VKATETTEQAITEKQQQVTEKQAIVDQKQQVADTAKKEKDTIDQSVKDQQAVVDQNKGA + LDQSQQAVTDQQAVVDEAKKVVDEATPSAIEKAKNQVATDTQAVDDQQKVVDQAQADVN + QQQAVVEEKAKETNAAKVQNDKDQQAVTAAKQEQAKLEELAKNAEVEKAKAEKEQAAKE + AELANKQKEEAKAKDQKTKDDQAVADQQTVVTTSQEKVADAKADTAAKQADLTAKENAL + KDKQTATKQVQNTLDKSKEELKGHKGINLPANFTPDYYKKLSEQEKQAMEKEALALNKV + FPENQADVAKATEMINVKNPTGKQKQQMSDYVVGLINDVREKLGLQKLKISNQAMRFAW + DVAKYDNPKEFDHDVNAINRAAKENGFKEYPGQNFYENLSMGYFETINGTISQLEFEKA + ARKTIADMLFDDESSAYSHIDSLLKGDTTNMAVSISGDLNDISAKIHIISYNQSKLVEA + NTYEEGTAPVFKSKETLQKEVATNQEKLATAQQAESDAQQAKSASQQVLNTAKTTQATA + EKELSVHKATLASLQAVATKSTTNYEEKVRQTATAEKSLQQTKDQLATINELIQNRAAV + LEKAKTNVSEAQAIEQTSAKVLKEKQAAQKAEENTLNSLKEVLNLAKENLNQKQVAFKT + STRSLSRLENAQPTYEKALNELNKAEAAAVQAQEAYENSLKSLEELKEQQAVATLAYTQ + AQEDLSNAKLELQQYQGVLRELEAQQAEQQRQEALQEQVAKEQQRLEREAKQSQTLVAS + ATSADKTPGLQQLSFSKQKEQPKAQALTHSESRKTKQVAKAPDSLPHTGEKNNKWLAIA + GLIFALLGAAGIISFISRNEKKVKNIFKIK" + gene 8603..12520 + /locus_tag="EF_RS00670" + /old_locus_tag="EF0149" + /old_locus_tag="EF_0149" + CDS 8603..12520 + /locus_tag="EF_RS00670" + /old_locus_tag="EF0149" + /old_locus_tag="EF_0149" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002395058.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="LPXTG-anchored aggregation substance" + /protein_id="WP_011109430.1" + /translation="MNQQTEVKKRFKMYKAKKHWIVAPILFLGVLGAVGLATDNVQAAE + LDTQPGTTTVQPDNPNPQSRNETLKTAVSEEAALQKDTTSQPTTAEEVVPKGIAAEQSS + ATSNDTTNVQQPTAEAEKSAQEQPVVSPETTIEPLGQPTEVAPAENDANKSTSIPKEFE + TPDVDKAVDEAKKDPNITVVEKPTEDLGNVSSKDLAAKEKEVDQLQKEQAQKIAQQAAE + LKAKNEKIAKENAEIAAKNKAEKERYEKEVAEYNKHKNDKGYVNEAISKDLVFDSSIVT + KDTKIDKITGGKFIKASDFNKVNQGQSKDIFTKLSKDMNGKATGNFQSSKVAAVEFGPK + GGYAVLLEKNKPVNVTYTGLNASYLDRKITKAEFIYELQSAPSQSGTLNAVFSNDPIIT + AFVGTKNANGKDVKVRLTIKLYDANGKEVLPDKDHAFAYALSSLNSSLGTNYSVEHAEF + VSDFGSKNEFKYINGSYVKKQADGKFYSTEDLDYGTGPSGLKNSDWDAVGHKNAYYGSG + VGLAREGGRISFSFGMTTKGKVNLSGAQWFAFSTNLNAKSIRPYQKKGNPKEPEKATIE + FNRYKANVVPVLVPNKEVTDGQKNINDLNVKRGDSLQYIVTGDTTELAKVDPKTVTKQG + IRDTFDAEKVTIDLSKVKVYQADASLNEKDLKAVAAAINSGKAIDVTASYVLNLDQNTV + TAMMKTNADGSVVLAMGYKYLLVLPFVVKNVEGDFENTAVQLTNDGETVTNTVINHVPV + SNPSKDVKADKNGTVGSVSLHDKDIPLQTKIYYEVKSSERPANYGGITEEWGMNDVLDT + THDRFTGKWHAITNYDLKVGDKTLKAGTDISAYILLENKDNKDLTFTMNQALLAALNEG + SNKVGKQAWSVYLEVERIKTGDVENTQTENYNKELVRSNTVVTHTPDDPKPTKAVHNKK + GEDINHGKVARGDVLSYEMTWDLKGYDKDFAFDTVDLATGVSFFDDYDETKVTPIKDLL + RVKDSKGADITNQFTISWDDAKGTVTISAKDPQAFILAYGGQELRVTLPTKVKANVSGD + VYNSAEQNTFGQRIKTNTVVNHIPKVNPKKDVVIKVGDKQSQNGATIKLGEKFFYEFTS + SDIPAEYAGVVEEWSISDKLDVKHDKFSGQWSVFANSNFVLADGTKVNKGDDISKLFTM + TFEQGVVKITASQAFLDAMNLKENKHVAHSWKAFIGVERIAAGDVYNTIEESFNNEKIK + TNTVVTHTPEKPQTPPEKTVIVPPTPKTPQAPVEPLVVEKASVAPELPHTGEKENTLLS + VLGAGMLVGLAWFGLKKREVK" + gene complement(12713..13570) + /locus_tag="EF_RS00675" + /old_locus_tag="EF0150" + /old_locus_tag="EF_0150" + CDS complement(12713..13570) + /locus_tag="EF_RS00675" + /old_locus_tag="EF0150" + /old_locus_tag="EF_0150" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359308.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359308.1" + /translation="MNIDFFNQLIKPVGILLPIVIGFFTIYSSIYGYVISDSFKKNLFS + SPTEIGLYKIFKGFINLISSLLLSSGIFAYALITTDTAFTDFVSDINNLTKEGKAYLTY + FIIYFIVFIFSIAKLPDIYRTLIPSNSRSKHSNFYVLSQELSLNDIPCNTKVFLVSMIN + KDTVLLTYYLNKEFVRLIYSLNEIQKIKIFYHKQLSFFQKIKEFNESITKETYYFAFLP + TIFIMLPSIIMSFISKSMTNIYFGATIAAISELIILSPSLIKYIKIAYKKIYLTIKNQN + KPKK" + gene 13629..14183 + /locus_tag="EF_RS00680" + /old_locus_tag="EF0151" + /old_locus_tag="EF_0151" + CDS 13629..14183 + /locus_tag="EF_RS00680" + /old_locus_tag="EF0151" + /old_locus_tag="EF_0151" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359309.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359309.1" + /translation="MLIKLGTELTKEEYVTRYMRNFQKLLLLGDRPKVLTNREEQLLQY + EKELCVLFYEQFIKKHHRAPDEATLDDQVKANFIERSKIFARSPLVMDEGNFTQAHIGQ + LKRLRELRMEDYLPDNYTHILQREEELARNYFRKHDDYPFGYECLCISRSREVVNQGLE + KLLEGFYDSYQVYYRRYRKNG" + gene 14226..14669 + /locus_tag="EF_RS00685" + /old_locus_tag="EF0152" + /old_locus_tag="EF_0152" + CDS 14226..14669 + /locus_tag="EF_RS00685" + /old_locus_tag="EF0152" + /old_locus_tag="EF_0152" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359311.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359311.1" + /translation="MAEESKYPPVKEKSVFYQRYSRVSTISMLMDLGFSVSEAQIDNED + IVYMEQYKEFLLNKGIVEEKVPFKREFVAEYPLSKMRRLFVEGYYLRKPSGEKMYGSYY + YDFYFKEYSGMGIYCTKATKSLFLHVFEQRIRKIKKLKHRLEE" + gene 14672..15520 + /locus_tag="EF_RS00690" + /old_locus_tag="EF0153" + /old_locus_tag="EF_0153" + CDS 14672..15520 + /locus_tag="EF_RS00690" + /old_locus_tag="EF0153" + /old_locus_tag="EF_0153" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_011109431.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="LPXTG cell wall anchor domain-containing protein" + /protein_id="WP_011109431.1" + /translation="MKKMIIIALFSTSLLAGGSSVSAYAQESEGNLGETTGSVLPDEPN + VPTDPITPSEPEQPTEPSTPEQPSEPSTPTEPSEPSKPTDPSLPDEPSVPTEPTTPSKP + EQPTEPTTPSVPEQPTEPSVPEKPVEPNKPTEPEKPVPVVPEKPVVPQQPEQPTDVVVK + PNGEIATGESTQQPTVPIETNNLSEVTHVPTVTTPIETASGEAIVAVDKGVPLTQTADG + LKPIKSEYKVLPSGNVQVKSADGKMKVLPYTGEKMGIIGSIAGVCLTVLSGILIYKKRK + V" + gene 15531..16520 + /locus_tag="EF_RS00695" + /old_locus_tag="EF0154" + /old_locus_tag="EF_0154" + CDS 15531..16520 + /locus_tag="EF_RS00695" + /old_locus_tag="EF0154" + /old_locus_tag="EF_0154" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002370321.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="conjugal transfer protein" + /protein_id="WP_002359315.1" + /translation="MKFLKKIQTKEKSKKSPRVRLLSQKKANRLVLIVGCALFGLSLIG + AIRANVMAGNVIRLSQQLETINKDVKKIKEKKRVYDSSALSFYVRNFVNEYINFNGKAT + DEEKKARDEKLASYFSGDLALDKGTDNAAKDLIRKLLQVSVTRVEESEELLLVHASISY + KVQQKGQDLLDTQEIVLPIQEKNGLFSIVGRPYFLAMNVPKGKQEPLQSVKEPLDVEKK + ERQGIEKFLQLFFEKYAKGNTEELSVLMKEPVKTSGQATVSSIEEKELRFFETKQKGVT + GVQVSVNFKDKAINFLYTEDFSLWLTKTENSYFVQELKHYYTEKAGDK" + gene 16520..16744 + /locus_tag="EF_RS00700" + /old_locus_tag="EF0155" + /old_locus_tag="EF_0155" + CDS 16520..16744 + /locus_tag="EF_RS00700" + /old_locus_tag="EF0155" + /old_locus_tag="EF_0155" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002370320.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359317.1" + /translation="MDTSKLSQFMSSGLITLIAFGVIILILKHWKEAAWLKIGSVIVIA + LILNDFATNNGQNIFKVVKWVLGLFGIQL" + gene 16757..17146 + /locus_tag="EF_RS00705" + /old_locus_tag="EF0156" + /old_locus_tag="EF_0156" + CDS 16757..17146 + /locus_tag="EF_RS00705" + /old_locus_tag="EF0156" + /old_locus_tag="EF_0156" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_016626678.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="conjugal transfer protein" + /protein_id="WP_002359318.1" + /translation="MKHFDYSRGLKAPYSLQVIKSPKGKIVWYFAQPLSLAYLVMLFLG + IVLTGIFWKFVPLPLIFGINLNLMIMLYFPNKVARWYTETEFEGKTGLAFLKDGFVYVK + NYVLDNRSIISFERVKEIEEFSFKR" + gene complement(17224..17532) + /locus_tag="EF_RS00710" + CDS complement(17224..17532) + /locus_tag="EF_RS00710" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002386531.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002386531.1" + /translation="MRKESVIKSFLYILIPIIIGTIISLFTSAPIFLIAGIIYIILLLF + LLPTLDFGITDFNAKQINPSYRPERKIDKNESIVTVLLLVIGIIVCAVMLYLKYRNS" + gene complement(17538..17756) + /locus_tag="EF_RS00715" + /old_locus_tag="EF0157" + /old_locus_tag="EF_0157" + CDS complement(17538..17756) + /locus_tag="EF_RS00715" + /old_locus_tag="EF0157" + /old_locus_tag="EF_0157" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359319.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359319.1" + /translation="MKKKVCLALTIFAMVTPFIFSSEASAREAEFGSSCPGGFYFKKGG + WSTGGKNMSGGYCVPLGNMGNMNNKIW" + gene 17920..20427 + /locus_tag="EF_RS00720" + /old_locus_tag="EF0158" + /old_locus_tag="EF_0158" + CDS 17920..20427 + /locus_tag="EF_RS00720" + /old_locus_tag="EF0158" + /old_locus_tag="EF_0158" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359320.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="ATP-binding protein" + /protein_id="WP_002359320.1" + /translation="MQLEYPLTNHMKRNMVLTKENQVIAYYRIRSETVGLTDFEKKRKT + KKKVARTLKRLQENEGFEIVLLPVNADIRGKMGGMRQLVDKENHSVAVDKLMKTAQYLE + NEIGMVYEYIWLIGVPLVKKERSIDIKETFSTALNNLSEKVVKGLGLEVGVAEDWEEAY + KDQEQEVYQNLSELLVERLTEDELYYYQAYQFLNNIQHEKKELLTSQNLDNLLANKITP + FRGGLELSNEFGKSYIAHLPLGDCGVTIDGNHLLELVQKMSYPVSVKLQAGFAETKGQL + ALSGRSARARTRTKNIMEEAHLAGSKQKRKIVEGQHSLDDLDQKIDDDIPIIDWKAVLV + ISGNSKKQLRERKKNLMNRLDSLGIPLIRATFDNVYLFQATLLGNFQRFSTSNWQHTST + LETFSELNFFTSLHAGTKTGFYLGRVDATLEEKESRKQIVSGSKNIVYMNLLLANKQNI + EGKKTNNPHWLVSGDTGNGKSVFSKWLFLYSSLLDVKVLYIDPKKEVRQQFMRTINDPE + YQRKYPLDVAFIKTFNFVTLDVRKKENHGVLDPIVLFDETEAIATAKAMLNNINEDKWK + MPHKTAINETVAEVVAERKAGKQVGFWHVIERLISHSEKDVHEMGRFLLSTIKGSILEL + AFSHGEVEGLSFEKKVTILEIEDLDLPTDRHDELDENQRLSVTLMFALGTFCSKFGSRN + RKEETVTFFDEAWIFQSSPEGQKILKSMKRIGRSFNNFMVLITQSVNDVLDNGDGTGFG + TVVCFDEVDNREGILRYLKLPVNEINLKWVSNMIQGQCLMKDMFNQVNRIVIDVIFEEW + LELFETVDDTEASVMENDYVARW" + gene 20434..22398 + /locus_tag="EF_RS00725" + /old_locus_tag="EF0159" + /old_locus_tag="EF_0159" + CDS 20434..22398 + /locus_tag="EF_RS00725" + /old_locus_tag="EF0159" + /old_locus_tag="EF_0159" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002403502.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359321.1" + /translation="MKYLIDSYTAYMVNGGLLDKGSEVVNWFFIELPFFFLRMGAMIFL + IMENVMNQSDYFVGKQQEAYDYSLDILKGFGGTGIVKGSLLGLAIILSAYYLLYSFFSN + RRNFMKSLLHYFAVFALFICWFGQVKTIDGKTQNGAIFLISSVSEMTKQVQGKFTSNVN + FGGDTSQEVDDGKKKVYQSPMFDATVLQTFNYVNSGSIDGKMANGKKLDYDKLLEKPNL + NDKEKKDFREKRNAYIEQQEKDNPYFSQDTLKTMEKSFAVWTGATNLFILAIPVLYINL + MLSLIQLLVVFLILIFPVVLLASFFPRCQMLLFKFFQGLIGALFTPIIYGIFLSVLFWI + NKLIDGAFLGVAKKVSGSLLELISGSTVYMIVLFVAVVVKIVVLRKVWKNKYAILAYFS + NGQVSQPVFEQKVEQLSERTKEVATGGAQVALGAYTGNLGMVANGAGNILPNQDKAIEM + GQEHFIDDNGSFTGVKAGLQSIFRQPVTEEEQGELNEEQFSEVETESIDHELTDIEEIG + QTELESDSLDNSIIELEPMTSEDLVVDVDNMSSDIDVELASVEEVGEEPPMNAEEYSGY + DNVIVTNFDELAMAREERDYFDGGKEQELVETNGVELVNNIYPFVQTEEYLANLEQTEA + QFFGTDEFEKELEQIEEWA" + gene 22429..23460 + /locus_tag="EF_RS00730" + /old_locus_tag="EF0160" + /old_locus_tag="EF_0160" + CDS 22429..23460 + /locus_tag="EF_RS00730" + /old_locus_tag="EF0160" + /old_locus_tag="EF_0160" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002386530.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="phage tail tip lysozyme" + /protein_id="WP_010775499.1" + /translation="MKKIFLGILGLFSFLFLLPFLLFLGTMSTEIGNNTQFQATTPQEK + VALEVSNFVTKNGGSLQFASAWLGNMEHESGLNPARIQSDLTFNSAWAFNPSTNGYALG + LAMMDGERRVNLLNFAKEQKKDWQAVPVQLEYMWNHDGSDSALLKRMSKSSDVNQLAVD + ILVHWERAGTKNDPNEQIKRKTSANNWYKRLSTGSMGAGSANIGGGKIDVLEQMLGQTV + NGGQCYGGTSYYVEKMGFQSLMNTGHMFASEIGNDYAWEQSGWQVIKNPNYSDVKAGDV + INFAMGGYATSVYGHTGIVASVEGNGKLVLYEQNAEKGQIIAKYFRQWGAEYPNVTSIV + RKK" + gene 23475..23765 + /locus_tag="EF_RS00735" + /old_locus_tag="EF0161" + /old_locus_tag="EF_0161" + CDS 23475..23765 + /locus_tag="EF_RS00735" + /old_locus_tag="EF0161" + /old_locus_tag="EF_0161" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359323.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359323.1" + /translation="MKKEEMDVVSLRIIKLYFLGIRELNFPDYNKRFQQKDMELFIQLA + DMMENLPNLDEQLIYELEELKDYLFYVKTEKYSLTVHDMFLEMKSELEKII" + gene 23807..24022 + /locus_tag="EF_RS00740" + /old_locus_tag="EF0162" + /old_locus_tag="EF_0162" + CDS 23807..24022 + /locus_tag="EF_RS00740" + /old_locus_tag="EF0162" + /old_locus_tag="EF_0162" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002393223.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_025186164.1" + /translation="MFRKKNGLTNRQVLAICTKFQCKKNEFVAHRLSDGYLVAVNNKEY + RVKFSEGFFSKIVYAKEVQRKGARKS" + gene 24019..24537 + /locus_tag="EF_RS00745" + /old_locus_tag="EF0163" + /old_locus_tag="EF_0163" + CDS 24019..24537 + /locus_tag="EF_RS00745" + /old_locus_tag="EF0163" + /old_locus_tag="EF_0163" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002370311.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="lipoprotein" + /protein_id="WP_002359325.1" + /translation="MNKLPLLILLLGGVLLVSGCQSHKEENKSSKVSTEETTVIETVAR + EQSKESFTSEATKKQTETTKLEEPDHVKLLEAYGNAYANFTSINDRNEKLKPLMTEKCI + KKNGIDVKTGVALVSVGKVTTIYKNDQHEYALLLDCEQNGTQTRVLLLAKVKNNKISEM + TYNSVKQEY" + gene 24611..24985 + /locus_tag="EF_RS00750" + /old_locus_tag="EF0164" + /old_locus_tag="EF_0164" + CDS 24611..24985 + /locus_tag="EF_RS00750" + /old_locus_tag="EF0164" + /old_locus_tag="EF_0164" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359326.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="lipoprotein" + /protein_id="WP_002359326.1" + /translation="MKKSIVTMLFLMLGIVVFTSCGSKMDKTAEEFKNKIISHVGDLYD + EKYQEKEFSFLIYKDKDTNEYLAKALVPYEGEPNSVESKYFYNVNKELETIEPFSGGRT + FDYAKSHGNYEVIYKSGKFK" + gene 25068..25280 + /locus_tag="EF_RS00755" + /old_locus_tag="EF0165" + /old_locus_tag="EF_0165" + CDS 25068..25280 + /locus_tag="EF_RS00755" + /old_locus_tag="EF0165" + /old_locus_tag="EF_0165" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_016623907.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="DUF3173 domain-containing protein" + /protein_id="WP_002359328.1" + /translation="MLIVTKEDLMNLGYGKYQAEDIIRKAKAVMVSKGYAYYLNKRLGR + VPVGAVESVLGIELESIAKEVETVG" + gene 25273..26439 + /locus_tag="EF_RS00760" + /old_locus_tag="EF0166" + /old_locus_tag="EF_0166" + CDS 25273..26439 + /locus_tag="EF_RS00760" + /old_locus_tag="EF0166" + /old_locus_tag="EF_0166" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002392915.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="site-specific integrase" + /protein_id="WP_002392915.1" + /translation="MVKFKKDKRGKYYFTASLGFDEVTGKRVQKMRSGFSTIKEAREAY + AEIINNFGKEAYSSNSTMLYEEFFYTIFLPYYKGRVKERTYNNRLSMIKNTFAYFFKMK + LKSIAPVHIQKWQNELLEQYENTYVRNIYGLFQMSLDRAVVLGMISSNPAKIVGNVKKS + KKEIDFWTKEEFEKVVKTFYVEDYYQNFSFICIWLLFMTGMRIGEATALTWKDVNLDRR + YLTVKKSLYYKNAQTYELVSPKTRASIRTIYLDEDTVHYLRDWKKRQDDVGGIEFILSY + NSVPTQKHTVRHIIKRHAKLAEVHDIRIHALRHSHASLLISMGTNALLIKERLGHEDVQ + TTLGTYGHLYPSSSTEIANELKGIVNVEFTNQNMASEVTNQFTKGVKK" + CDS 1..1511 + /origid="WP_002359295.1" + /function="Coupling Protein" + /protein_id="WP_002359295.1" + /locus_tag="EF_RS00630" + /codon_start=1 + /color=6 + /note="ICEscreen prediction: Coupling protein; BlastP + result (Annotation confidence: HIGH): Coupling protein TcpA + [Hit with AAO80014; Identity:100.00%; E-value:0; Query + coverage:100.00%]; Hmmscan result: TcpA [Hit with tcpA HMM + profile; E-value:0; i-Evalue:0]" + CDS 3098..4380 + /origid="WP_002359299.1" + /function="Relaxase" + /protein_id="WP_002359299.1" + /locus_tag="EF_RS00650" + /codon_start=1 + /color=7 + /note="ICEscreen prediction: Relaxase; BlastP result + (Annotation confidence: HIGH): Relaxase MOBT (PF02486) [Hit + with AAO80018; Identity:99.77%; E-value:0; Query + coverage:95.53%]; Hmmscan result: MOBT [Hit with T4SS_MOBT + HMM profile; E-value:0; i-Evalue:0]" + CDS 17920..20426 + /origid="WP_002359320.1" + /function="VirB4" + /protein_id="WP_002359320.1" + /locus_tag="EF_RS00720" + /codon_start=1 + /color="184 134 11" + /note="ICEscreen prediction: VirB4; BlastP result + (Annotation confidence: HIGH): VirB4 [Hit with CAE52368; + Identity:41.15%; E-value:0; Query coverage:99.76%]; Hmmscan + result: VirB4 [Hit with T4SS_virb4 HMM profile; E-value:0; + i-Evalue:0]" + CDS 25273..26438 + /origid="WP_002392915.1" + /function="Tyrosine Integrase" + /protein_id="WP_002392915.1" + /locus_tag="EF_RS00760" + /codon_start=1 + /color=8 + /note="ICEscreen prediction: Tyrosine integrase; BlastP + result (Annotation confidence: HIGH): Tyrosine integrase + [Hit with AAO80040; Identity:100.00%; E-value:0; Query + coverage:100.00%]; Hmmscan result: Tyrosine integrase [Hit + with Phage_integrase HMM profile; E-value:0; i-Evalue:0]" + mobile_element 1..26439 + /mobile_element_type="other: integrative and conjugative + element" + /note="ICEscreen prediction: Putative ICE (one integrase - + Tyr) [Element structure: Single; ICE superfamily: Tn916; + ICE family: ICESt3; Relaxase family: MOBT; Coupling protein + family: TcpA] (ICEscreen ID: 2)" + /color=15 +ORIGIN + 1 atgttaaaaa aattatttag atatagagga aggcgtattc gttattcttc aagaaacctg + 61 ttggcactct atcgagtgtt attttttatg ccaattttag cttgtttagg gtattttgtt + 121 ggctataaat ggatttatcc tttatatctt tcaaatccgc ctgattggaa aatatacatt + 181 gtgccagcgt tgataattgt aggtatttct ataggtgcaa ttgtgctaat tacattgctt + 241 attaaagctt caattattaa ttcggggtat ttttctaaag tggaacaacg tcaagtatta + 301 gcacatatga ttattgacaa cgggtattac acaaaaaaac aagtgaaaag tagtgacggc + 361 aaaacaaaag aaaaaatcaa gtttccaaaa atttattata agtctgcaaa aaatagtatt + 421 tttgtttcat ttgaaacagc gggtaataaa tttcaggaaa aatttgaaac cattggtggt + 481 tttttggaaa caacgtttca tgcagataat ttaaataaga ttgatgaaaa aggttttgtt + 541 acctatgaat tagcaacaga tgtgtacaat aagcgtattt ggattaagga tatgcaagca + 601 gacgaaggca aagttcagtt gatgaaaggg ttgtattggc actttgataa agatccacat + 661 ttgttattag gtggaggcac tggtggaggt aaaactttta caattttgtc tctgatctat + 721 gcgctttgtc gagtgggtga ggtggaaatc tgtgacccga aaaattctga cttgatggct + 781 ttaggcaagt taccgctatt tgcaggaaaa gttcatactg gaaaaaaaga tattactcaa + 841 tgcttggaaa atacagtgga gttaatggaa acacgtttta aaacgatgaa taatagttca + 901 cgttacaaga tggggaaaaa ctatgcgtat tatggattaa aaccaaaatt tgtttttata + 961 gatgaatttg cggcctttaa agctgaattg gcaaatgatt attcaacaga cggagaagta + 1021 gatgagtatc ttacgcaact tattttgaaa gctcgtcaag caggtatttt ctttattgtt + 1081 gcaatgcaac gtccagatgg agaattttta aaaacagcat tacgtgacca atttatgttt + 1141 cgcatgtctg ttggacgtct atctgaaaca ggtattttga tgattttcgg tgatgaaaat + 1201 aaaaataaaa agtttaaata tgttgaaaaa attgacggtc agaaagtcta tggtcgtgga + 1261 tatgtggctc aaggaggagg aactgcacgt gaattttata gtcctcaagt accacaagac + 1321 tttgacttca ttgaagaatt tatcaaaatt tctaaagagt taggttatga agatgttcca + 1381 aaagaagtac aagaagaagt atctcaaaaa atatctaaac atattgataa agaagcactg + 1441 gcagaaattg atgaagaatt tcaagcagaa aaaaatcagt tatcagagtt aagtgagaaa + 1501 tattctgctt aaaaagaagt cacataaatt agttggaaaa ggaaggtttt tatggattct + 1561 aatgaaataa ttaaacgtgt cagagaacga gtatatagag aagtaaagaa aaaatatact + 1621 agagacgatt tggatactcg tattcaagat gtattatatt atcgttcaga aacatatatg + 1681 aaattagtta gctttgcaaa tggtaaaaga attaagaagt tagctgatcc tagaaaattt + 1741 gaaaagttta tggatacaaa aggagtaaag attgttgcag aagtacttga tggattgaat + 1801 aatcaaccaa aaatgcaagc tatggaatac gaacaaaagg tacttactaa agtacgtcaa + 1861 tggtatcaga aaaagaatca ccctgagctt gtagatttgg aagaagaagc atttgaacaa + 1921 cttgtagaaa aaaatatcat ctataaaaaa atgaagaaaa ggttgtatga agaacaggat + 1981 aatcaaggtt ttgtttattc ggataatttt gatatgcaat tgattcgtga ttcatgtgat + 2041 attgaagagg ctctatactt agatatcacg ttgggggatt actaagtgaa aggagtaaaa + 2101 agaaagtgaa atttaaatct cgagtacaaa tgttaaacta tgtgattggg aagaaaccag + 2161 acatcatgaa aacagttgat cggagagcag aaaaagaggg ggttacagat gataatagat + 2221 tacttgatta taaattatta gtaatttctg ttttagatgc agaagctgtt tacgatgaag + 2281 atagaccaat tgaggaaaag aaattttcaa catcacaaga agttgataag tatttagaaa + 2341 caagatttaa ggagcatatg gattctgctt ggaaagaaat gtttaataag taggtgtgga + 2401 aaatgtattc atttgatcaa gtaattttag agctagacaa tgctattgct ttgaaaacac + 2461 tgaaaaattg ggcaaatagg attgaaaaaa tgactgatac acgttttgaa cgccgatatg + 2521 caaagaatag tgcaggtcat agttatagct ataaggtttt ttctattaaa gatatagaaa + 2581 attttcaaga gttgctacgt ttgcgtgaaa ataatgttcc gcttaatgag gcaataaatg + 2641 aagtatttat gtctgatgag aataaagcta cacatattaa taaacaagaa attgaggatt + 2701 tgaaaagaga tatgaggcaa cttttaaagg tgtctcaatc tattcttgcg gagaatgcaa + 2761 gcttaaaaga gagaattggt gcattagaac gaggaacagc tgaataacgg ctgtttattt + 2821 gaaggattgt tgctagtaat aagtatacgt gggcgaagca gagcgaaagc ccacgtatac + 2881 ttttagcaac ggaaaatcct tttaaaatga acagacgtaa ggtgggcgaa tgcccacctt + 2941 acgtcaaaaa ccccctattt ataacatggg ggtagaattt aatataccgt ataaaaaagt + 3001 tgcttaaaag ctatgtccta caagggattg agcgattatg attttgtcac atttttcgac + 3061 taaagttgtc acattttttg gaagtaggtt taaagcagtg agaggtgttg atttaaagcg + 3121 atatagaaaa gaactaaaat taaaacagca agaattggca agtaaattag gaatcgagcg + 3181 ttcgttaatt tcaaaaatag aatctggaaa acgtgttatt tctaaagagt tagaacaaaa + 3241 gattattaat gtgttgaact tagatggtgg gcacgcttcg gtagaagcaa aaattgactt + 3301 tttaagaata cgatttaaaa cattagatgt tcggacagtt attgaaaagt tgttgcacat + 3361 ggatatgaac tggtttactc atgaatctag agggttttat cattacacgg aaacattttc + 3421 atatagctct attcggattt ttcggaatcc ggaaaatgtc aatatgggaa ttatgttgga + 3481 cttgtctggc gaaggctgtc gtcaattaga agaaatcttt gaggaagata acaaccgatc + 3541 gtggacagaa ttttttcgct ctctttatga tgacgatatt ttcggtcaag gcattttagt + 3601 agatacaaaa atcactcgaa ttgatattgc tcttgatgaa ttgattgtga aggggcaaga + 3661 aaattttgat ttgtatgttt taaaagaaaa aatggagcag gggcttgttg acaccacatt + 3721 taaaaatttt gattttagcg gtggatttgt ttacgaaaat aaaaaaatgg tgaataaagg + 3781 tttatccctt tattttggta gtcgtcaatc gccactgtat tttaactttt accaaaaaga + 3841 ttacgagtta gcaagaaaag aaagtatgag tgttgaagaa gcaagagaaa aacatgaaat + 3901 taaaaatcga tacgaaattc gcctttctga tgaaaaggcg tttttgtttg tagaatattt + 3961 tctatcaagc ggtgagtcgc tagattggtt agtgaaagaa attattaatc aatctttgac + 4021 tgtctatgac attgaagatg atatgaaagt ttattgtaaa tcttggcatg atgttgttga + 4081 taaattggaa ggcttaaaat tgtctgtgca aggtgaaaaa ccgtctattg aaaagacgct + 4141 tcgatggcta tcaaattatt tagccccttc attaaagatg attaaagaga ttgataatct + 4201 gttaggaaca aatgaattga tggaacgcat tgatttagca gaattaaaag aaaagcatga + 4261 agagattatt gaaatggtga gtgttgatgc aaaagacttg ttgtttacca atagagataa + 4321 taaaagtgtt cggtcctata tggaacgaga gttcgaatta gaagaaatgt acccgtttta + 4381 gaaaggtgga ttgatagaat ggataaagaa ttaatcaaaa aaaatataaa acttatggat + 4441 gccactattg agaaaatgat tcatgtgatg agttctattc ataaacttca attgagtgta + 4501 aaagaacttg aagacagtaa cgaattaact gtttcttatg taaaaagaga atgtacaaaa + 4561 ttggaagaga gggttaagga acttagaccg agtatttcaa gatgtgtttt tatgtatcga + 4621 gaatatgtag aagaatttga aacaaaactt agagatagaa tagttacaga aaagtttttt + 4681 cgaataaaaa ggttctatgg taaagaagta ttagctttta aggaatttca agaaaagtat + 4741 caaaattata ttcctaaaga tattattgat ataaagaaac aagttcgtca gaagctagaa + 4801 gaagttggtt atgaaattga tggtgctttt gagggggatt ttacaagttg ggtaggtgtt + 4861 tatgcgcgtc caaaagataa accgacctat ttagaccctg cgaatgcgga agaagtagtt + 4921 ttacaagaaa aatatagcgt caatggattt aaacaagatt tttcagaatg gtttgaattt + 4981 gaaattaaag ataatgttgt ttatggcatt taataattgt catgtaaaag acatttttta + 5041 ttttctctaa aaggttacta ttttcataat aaatattatt ttgttattgt taatttgagg + 5101 tgaaaggggt atgactaaaa aattaataaa aatatggcgt aaagaaatgt tggcattaat + 5161 gaatgaagac gcagactggt ataggaatga agataccgag cgttttaaac gaatacaaga + 5221 acttgcaaga aagatagaaa cagcaaacac cagacaattt tcttcgcata ttagcaagga + 5281 aagatttgaa gcctatcaaa aaatgggctt acagtttaaa gaaatagcgg aagagttcca + 5341 tataacaact acagcgttac aacaatggcg taaagacaat gggtatccca tttacaacaa + 5401 aaataatcgg aagtgaggaa aataaaatga aaaagatcgc aagtgcaggg ttaagtattt + 5461 tagtcgcaac gggggtagca ggtattgggg gaaatgaagt acaggcagca gaacaagcgc + 5521 aaccaaaaac acctgaaaac agttctacag aacaaccagc agtgaaagct acagaaacaa + 5581 cggagcaagc cattactgaa aaacagcaac aagtaacaga gaaacaagca attgtcgatc + 5641 aaaaacaaca agttgctgac actgcgaaaa aagaaaaaga caccattgat caatctgtta + 5701 aagaccaaca agcagtggtc gatcaaaaca aaggtgcatt ggatcaaagt caacaagcag + 5761 tgactgacca acaagcggtc gtagacgaag caaaaaaagt tgtggatgaa gcaacacctt + 5821 cagccattga aaaagccaaa aaccaagtgg ctacggatac acaagctgtt gatgaccaac + 5881 aaaaagtagt ggatcaagct caagcagacg ttaaccaaca acaagcagtt gtcgaagaaa + 5941 aagcaaaaga aacgaatgct gctaaagtgc aaaatgataa agatcaacaa gcagtaacag + 6001 ctgcgaaaca agaacaagcc aagcttgaag aattagcgaa aaatgcggaa gtggaaaaag + 6061 caaaggctga aaaagaacaa gcagcaaaag aagctgaact ggctaacaaa cagaaagaag + 6121 aagccaaagc taaagatcaa aaaacgaagg acgatcaagc agtcgcagac caacaaactg + 6181 ttgtgacaac tagtcaagag aaagtagcag acgcaaaagc agatacagct gcgaaacagg + 6241 cagacttaac agcgaaagag aatgctttga aagacaagca aacagcaaca aagcaagtgc + 6301 agaatacttt agataaatca aaagaagagc taaaaggaca taaaggaatt aacttgcctg + 6361 ctaacttcac acctgattat tacaaaaaat tatctgaaca agaaaagcaa gcaatggaaa + 6421 aagaagcatt agcattaaat aaagtttttc ctgaaaatca agcagatgtg gcaaaagcaa + 6481 cggaaatgat caatgtcaaa aatcctaccg gaaaacaaaa gcaacaaatg agcgattacg + 6541 ttgtaggact tatcaatgat gttcgcgaaa agcttgggtt acaaaagttg aagatttcta + 6601 accaagctat gagatttgct tgggatgtag caaaatatga taatcccaaa gagtttgatc + 6661 atgacgtaaa tgcaattaat cgtgcagcaa aagaaaatgg ttttaaagag tatccagggc + 6721 aaaactttta tgaaaatcta agtatgggct attttgaaac gattaatggc actatttctc + 6781 aactagagtt tgaaaaagct gctcgaaaaa caattgctga tatgctcttt gacgatgaaa + 6841 gttcagcgta ttctcatata gattcattgc taaaagggga cacaacaaac atggcagttt + 6901 ctatttcagg agatttaaat gatatttcgg caaaaatcca tatcattagc tataatcaat + 6961 caaaattagt tgaagcaaat acctatgaag aaggcactgc tccagtcttt aagagtaaag + 7021 aaacccttca aaaagaagta gcaaccaatc aagaaaaatt agctactgca caacaagcag + 7081 aatcagacgc tcaacaagca aaaagtgcaa gtcagcaagt cttaaataca gccaaaacaa + 7141 cacaagcaac agcagaaaaa gaactatctg ttcataaagc gacattggct agtcttcaag + 7201 cagttgcgac taaaagtacg acaaattatg aagaaaaagt acgacaaact gcaacggcag + 7261 aaaaaagcct tcaacaaaca aaagatcaat tggcaacaat caatgagtta attcagaatc + 7321 gagctgctgt tttagaaaaa gcgaaaacaa acgtttcgga agcacaagca attgaacaaa + 7381 cgtctgctaa agtgctgaaa gaaaaacaag cagcccaaaa agcagaagaa aacacattga + 7441 atagcttgaa ggaagtattg aatttagcaa aagaaaattt aaatcaaaaa caagttgcat + 7501 ttaaaacaag tacacgttca ttgtctcgtt tagaaaatgc tcaaccaaca tacgaaaaag + 7561 cattaaacga gttaaacaaa gcagaagcag cagcggtcca agcacaagaa gcctatgaaa + 7621 attctctgaa atcattggag gaacttaaag aacaacaagc cgttgctaca cttgcttata + 7681 cacaagcaca agaagacctt tctaacgcga agttagagct acagcagtac cagggcgtat + 7741 taagagaatt agaagcacaa caagccgaac agcagcgaca agaagcgttg caagaacaag + 7801 tagcaaaaga acaacaacgc cttgaacgag aagcaaaaca aagccaaacg ttagtagcaa + 7861 gtgctacttc agcagacaaa acacctggtc tccaacagtt atctttttct aaacaaaaag + 7921 aacagccaaa agcacaagca ttaacacatt cagaatctcg taagacgaaa caagtagcaa + 7981 aagccccaga ttctttacca catacaggag aaaaaaataa taaatggtta gctatagctg + 8041 gtctgatatt tgctttgtta ggagctgcgg gtattataag ttttattagt agaaacgaga + 8101 agaaagtaaa aaatatcttt aaaattaaat aataaagtca aaggagcgac atatttcgct + 8161 cctttttatt gtaatcattg gaataaattg aaaaataaaa aaatagccaa ccaaaatggt + 8221 tggctgattg tattaaaggc agaagaatct accaaattta ggagccaacc agtttccgct + 8281 ggtcaacaaa aaataaatgg attgtctcgg atggagacac ttaaatagta cctgacaata + 8341 aaattttagg caacaaaaaa gtcagccgtt ttccgccgac tgactaaacg aataagattt + 8401 gtaaagaagt gatttgtact ttttacacaa ttatgatagc acgttaaatg tttttaacgc + 8461 aaaaaaaagc taagggaatc gaaaaacttt cggttctctt tttgtgagga ggaatggaaa + 8521 taatgatgaa aaagcaacga gctccttgtg ttgcgtgaac tatttttaca aacaaaaaat + 8581 aaaaaatcga ggagaatgat acatgaatca acagactgaa gtaaaaaaac gttttaaaat + 8641 gtataaggca aagaaacact ggattgtagc ccctattctt tttttaggtg tattaggagc + 8701 tgtaggatta gctacagata atgtacaagc cgcggaatta gatacgcaac caggaacaac + 8761 gacggtgcaa cccgataatc ccaacccgca gtcacgaaat gaaacactta aaacggcagt + 8821 atctgaagaa gcagcattac aaaaagacac tacttctcaa ccaaccacag cagaagaagt + 8881 agtgccgaaa ggaattgctg ctgaacaaag ttcagctacc tcaaatgata ccacaaacgt + 8941 ccaacaacca acagcagaag cagaaaaatc agcacaagaa caaccagtag tcagccctga + 9001 aacaaccatt gaacctctag ggcagccaac agaagttgca ccagcagaaa atgacgctaa + 9061 taaatcaacg tcaattccta aagaatttga aacaccagac gttgacaaag cagttgatga + 9121 agcgaaaaaa gatccaaaca ttaccgtcgt ggaaaaacca actgaagact taggaaatgt + 9181 ttcttctaaa gatttagctg caaaagaaaa agaagtagac caactacaaa aagaacaagc + 9241 ccaaaagatt gcccaacaag cagctgaatt aaaagccaaa aatgaaaaaa ttgccaaaga + 9301 aaatgcagaa attgcggcaa aaaataaagc ggaaaaagag cgctacgaaa aagaagtggc + 9361 ggaatacaac aaacacaaaa atgacaaagg ctatgtgaat gaagcaatca gcaaagactt + 9421 agtttttgat tcaagcattg tgactaagga tactaaaata gacaagatta caggtggcaa + 9481 gtttattaaa gcttccgatt ttaacaaagt aaaccaagga caatccaaag atatttttac + 9541 aaaattaagt aaggatatga atggaaaagc aacaggcaac ttccaaagct caaaagtggc + 9601 tgctgttgaa tttggcccta aaggaggata cgcggttctt ttagaaaaaa acaaaccggt + 9661 caatgtcacg tatacaggat taaatgctag ttatttagac cgaaaaatca caaaagcaga + 9721 gttcatttat gaacttcaat ctgcaccaag tcaaagcggc accttaaatg cagtattttc + 9781 gaatgatcct attattacag catttgttgg tactaaaaat gccaatggga aggacgtaaa + 9841 agttcgctta accattaaat tatatgatgc taatggcaaa gaagttttac cagataaaga + 9901 ccatgccttt gcctatgcgt tatcgtcatt aaactctagt ctaggaacaa attatagtgt + 9961 agaacatgcg gaatttgttt cagactttgg ctcaaaaaat gagttcaagt atattaatgg + 10021 ttcctacgta aagaaacaag cggatgggaa attctactca accgaagacc ttgattatgg + 10081 gacaggtcct tcaggactaa aaaatagtga ttgggacgct gtaggtcata aaaatgcgta + 10141 ctatggttct ggtgtaggtc tagctagaga gggtggacgt atttctttct cttttggtat + 10201 gacaaccaaa ggaaaagtca acctatctgg tgcgcaatgg tttgccttta gtaccaattt + 10261 aaatgcgaaa tcaattagac cataccaaaa gaaagggaat ccaaaagaac cagaaaaagc + 10321 aacaattgaa ttcaatcgat acaaagccaa tgtcgttcct gttcttgtgc cgaataaaga + 10381 agtcactgat ggtcagaaaa atatcaatga tttaaatgtg aaacgaggcg attctttaca + 10441 atacattgtg acaggggata cgacagaact tgccaaagta gatccgaaaa cagtgacaaa + 10501 acaagggatt cgggatacct ttgatgcaga aaaagtgacg attgatttat ccaaagtgaa + 10561 ggtttatcaa gcagatgcaa gtctaaacga gaaagactta aaagctgttg ctgcagcgat + 10621 taattcagga aaagctatag acgtgactgc ttcttatgtt cttaatttag atcaaaacac + 10681 cgtcacagca atgatgaaaa ccaacgcaga cggttccgtt gttttagcaa tggggtataa + 10741 atatttactt gtcttgccgt ttgtagtgaa aaatgtagaa ggcgattttg aaaatacagc + 10801 tgttcagctg acaaatgatg gcgaaacggt aacaaataca gtgattaacc atgtgccggt + 10861 tagtaatcct tccaaagatg taaaagcaga taaaaacggt acagttggca gtgtttctct + 10921 acatgataaa gatattccgt tacaaacaaa aatttattat gaagtgaaat cttccgaacg + 10981 tccagctaac tatggcggaa ttaccgaaga atggggcatg aatgatgtct tggacacgac + 11041 ccatgatcgt ttcacaggta aatggcacgc tattacaaac tatgacctta aagtagggga + 11101 caaaacgtta aaagcaggaa cagatatttc tgcctacatt cttttagaaa acaaagacaa + 11161 taaagacttg acgtttacaa tgaatcaagc attattagca gcgttaaatg aaggaagcaa + 11221 taaagtaggc aaacaagctt ggtctgtata tctggaagtc gaacggatca aaacaggtga + 11281 cgtagaaaat acgcaaacag aaaactacaa caaagaactt gttcgttcta atacggtggt + 11341 gacgcataca cctgatgatc caaaaccaac caaagccgtt cacaacaaga aaggggaaga + 11401 tattaaccat ggaaaagttg ctcgtggtga tgttctttct tatgaaatga cgtgggactt + 11461 aaaagggtac gataaagact ttgcttttga cacagtcgat cttgcgacag gagtttcttt + 11521 cttcgatgat tacgacgaaa cgaaagtgac accaatcaaa gacttacttc gtgtcaaaga + 11581 ttctaaaggg gcagacatta cgaaccagtt cacgatctct tgggacgatg ccaaaggcac + 11641 ggtgacaatc tctgccaaag acccacaagc ctttattcta gcgtatggtg ggcaagaatt + 11701 gcgtgtaacg ctccctacaa aagtcaaagc caatgtttct ggtgatgttt ataattcagc + 11761 ggaacaaaat acatttggtc aacgaattaa aacaaatacc gttgtcaacc atattccaaa + 11821 agtgaaccct aaaaaagacg tggttattaa agttggtgac aaacaaagtc aaaatggcgc + 11881 cacaatcaaa ttaggggaga aattcttcta tgaatttaca agtagtgaca ttcctgcaga + 11941 atacgctggt gttgtggaag aatggtcgat tagcgataaa ctagacgtaa aacatgacaa + 12001 atttagtggc caatggtctg tgtttgccaa ttctaatttt gttttagcag acggaaccaa + 12061 agtgaataaa ggggacgaca tttcgaaact attcacgatg acctttgaac aaggggtagt + 12121 taaaatcacg gccagtcaag cgtttttgga tgcgatgaat ctaaaagaaa acaaacacgt + 12181 tgcgcactca tggaaagcgt tcattggtgt agaacgaatt gcggcaggag acgtttacaa + 12241 cacaatcgaa gaatctttca acaatgagaa gattaaaact aatacggtag tgacacatac + 12301 gccagaaaaa ccacaaacac caccagaaaa aacagtgatt gtaccaccaa caccaaaaac + 12361 accacaagca ccagtagagc cattagtggt agaaaaagca agtgtggcac ccgaactacc + 12421 tcatacaggt gaaaaagaaa acaccctatt atctgtacta ggtgccggaa tgctagttgg + 12481 tctggcttgg tttggtttga aaaaacgtga agtgaaataa tgagtaaaaa gaaaaagcac + 12541 tgtgaaaaat cagtgctttt tgtttatttt agaaaaagtg tggtgttgtc aagtgtggat + 12601 aataattttt gcgttaagtt gtatcgtagt atctataata attggagcat ttgtcagtgc + 12661 aacggtcgat gaacgattgg gagacaatat ttttttgcta cttctagtta ttttattttt + 12721 ttggtttatt ttgattttta attgttaagt atattttttt atatgctatt ttaatatatt + 12781 taataagact aggtgaaagt attattaatt cagagatagc tgctattgta gctccgaaat + 12841 aaatattggt catacttttt gaaataaacg acattattat agaaggtagc attataaata + 12901 ttgttggcaa aaaggcaaaa taataagttt ctttggtgat agattcgtta aattctttga + 12961 ttttttgaaa gaaagatagt tgtttatgat agaatatctt aattttctgt atctcgttta + 13021 aagaataaat taagcgcaca aattctttat ttaggtaata agttagaaga acggtatctt + 13081 tgttaatcat tgaaactaag aaaacttttg tattacatgg gatatcattt aaagaaagtt + 13141 cttgggacag tacgtaaaag ttactatgct tacttcttga attagaaggt attaaggttc + 13201 tataaatatc agggagcttt gctattgaga aaataaatac aataaaataa atgataaagt + 13261 aagttaagta agctttaccc tctttagtaa ggttatttat atctgaaaca aaatcggtga + 13321 atgctgtgtc tgtagttatt aatgcataag caaaaatacc agatgaaagt agtaaagagg + 13381 atattagatt aataaaacct ttaaagattt tatataatcc gatttcagtg ggagaggaaa + 13441 aaaggttttt tttaaatgaa tcacttatta cgtatccata gatggaggaa taaattgtga + 13501 aaaagcctat aactataggt aaaagtatac ccactggttt tattaattgg ttaaaaaaat + 13561 caatgttcat ataaataatt actccttata aactttttat aataattata tcaaaaggac + 13621 gtgaatatat gttaataaaa ttaggtacag aattgacgaa agaagaatat gttacaaggt + 13681 atatgcgaaa ctttcagaaa ttattgttgc tcggcgatcg gccaaaagta ttaacgaatc + 13741 gtgaagaaca actcttgcag tatgaaaaag aattatgtgt gctgttttat gaacaattta + 13801 taaagaaaca tcatagagcg cctgatgaag ccacgctaga cgatcaagtt aaagcgaatt + 13861 tcatagagcg ctctaaaatt ttcgctcgat ctcccttagt aatggacgaa ggaaatttta + 13921 cgcaagcaca tattggtcaa ctaaaacggt tgcgtgaatt gcgcatggaa gactatttgc + 13981 cagacaatta tacgcatatt ttacaacgag aagaagaatt ggcacgtaac tattttcgaa + 14041 aacatgatga ttatccattt gggtatgagt gtttatgtat tagtcggtca cgagaagtgg + 14101 tgaaccaagg gctagaaaag ctcttagagg ggttttatga cagttatcag gtttattatc + 14161 gtcggtatcg taaaaatggt tagaaatgga agtaggaata aaaagcgcag gaggtgtagc + 14221 gagcaatggc agaagaatcc aaatatccgc cagtgaaaga aaaatctgtg ttttatcagc + 14281 gttatagtcg tgtttcgacg atttctatgt tgatggattt aggttttagt gtgagtgaag + 14341 cccagattga taatgaagat attgtttata tggaacagta taaagagttt ttattaaata + 14401 aaggaattgt ggaagaaaaa gttcctttta aacgagaatt tgtagcggag tatccattaa + 14461 gcaaaatgcg acgacttttt gtagaaggct attatttacg aaaaccgagt ggtgaaaaaa + 14521 tgtatggtag ctactattac gatttttatt ttaaagagta ttcaggaatg gggatttatt + 14581 gtacgaaagc aacaaaatct ctttttttac atgtttttga acaaagaatt agaaaaataa + 14641 aaaaattaaa acatagattg gaagaatgaa aatgaaaaaa atgattatta ttgccttatt + 14701 cagtacaagc cttttagcag ggggaagcag tgtttctgct tatgcgcaag aatcagaagg + 14761 aaatcttggt gaaacaacag ggagtgtttt accagatgaa ccgaatgtac caactgaccc + 14821 aataacgcca agtgagccag agcaaccaac agagccaagt acaccagagc aaccatcgga + 14881 accgtcaaca ccaaccgaac ctagtgagcc ttcaaaaccg acggatcctt cgttaccaga + 14941 cgaaccgagc gtaccaacag agccaacaac gccaagtaag ccagagcaac caacagagcc + 15001 aacaacgcca agtgtaccag agcaaccaac agagccaagt gtaccagaaa aaccagtaga + 15061 accaaataaa ccaaccgagc cagaaaagcc tgtgccagtt gttcctgaaa aaccagttgt + 15121 accacaacaa ccagagcaac caacagatgt ggtggtaaag ccaaatggag aaattgcaac + 15181 aggagaatct acacaacagc caactgttcc aattgaaacg aataaccttt cagaagtaac + 15241 acatgtccca actgtgacga caccgattga aacagcaagc ggagaagcaa ttgtcgcagt + 15301 ggataagggc gttcctttaa cacaaacggc tgatggatta aaaccgatta aaagtgaata + 15361 taaagtatta ccaagtggca atgtacaagt gaaaagtgct gacggaaaaa tgaaagtact + 15421 tccttacact ggtgaaaaaa tgggcataat tgggtcaatc gctggtgtat gtttgactgt + 15481 tttatcagga atcttaattt ataaaaaacg taaagtgtag tgagtgatgt atgaaatttt + 15541 taaaaaaaat tcaaacaaag gaaaaaagca aaaagagccc aagagttcgt ttactatcac + 15601 aaaaaaaagc gaatcgatta gtattaattg tggggtgtgc tctttttgga ttgtcattga + 15661 ttggtgctat tcgagcaaat gtgatggcag gcaacgtgat ccgcctcagt caacaattag + 15721 aaacaataaa taaagatgtc aaaaaaataa aagaaaaaaa gagagtgtat gattcgtcgg + 15781 cactctcttt ttatgtcaga aattttgtga atgagtatat caattttaat ggaaaagcaa + 15841 cagatgaaga gaaaaaagca cgtgatgaga aattagcgag ttatttttca ggagatttag + 15901 cgttagataa aggaacagac aatgctgcaa aagacttaat tcgtaaatta ttacaagtct + 15961 ctgttactcg tgtagaagaa tctgaagagc tattacttgt gcatgcgagt atatcttata + 16021 aagtgcaaca aaaagggcag gatttactag atacacaaga aattgtttta cctattcaag + 16081 aaaaaaatgg gttgttttct attgttggtc gtccctattt tttagcaatg aatgttccga + 16141 aaggaaaaca ggaaccattg caatcagtga aagaaccgct tgatgttgaa aaaaaagaac + 16201 gacaagggat tgaaaaattt ttacaattat tttttgaaaa atatgcgaag gggaatacag + 16261 aagaattgtc tgttttaatg aaagaaccag ttaaaacaag tggtcaagca actgtgtcgt + 16321 ctattgaaga aaaagaacta cgtttttttg aaacgaaaca aaaaggtgtt acaggcgttc + 16381 aggtgtcagt gaactttaaa gacaaagcaa taaatttcct ttacacggaa gatttttcgt + 16441 tgtggttaac gaaaacagaa aattcgtatt ttgtacaaga gttaaaacat tattatacag + 16501 aaaaggcagg agataaatag tggatacaag taaattaagt caatttatga gtagtggatt + 16561 gattacactt attgcgtttg gtgtgatcat tttaatttta aagcattgga aagaagctgc + 16621 gtggttaaag attggttcag ttattgtcat tgcattgatt ttaaatgact ttgcgacaaa + 16681 taatgggcag aacattttca aagttgtgaa gtgggtctta ggattatttg ggattcagct + 16741 gtagaggaga gaaataatga aacattttga ttattcgagg ggcttaaaag ccccttactc + 16801 attacaagtt attaagtccc caaaagggaa aattgtttgg tattttgcac aacccttaag + 16861 tttagcttat ttagtgatgt tatttttagg gattgtacta acaggcattt tttggaagtt + 16921 tgtaccattg ccgttaattt ttggtattaa tcttaaccta atgattatgt tgtattttcc + 16981 aaataaagtg gctcgatggt atacagaaac agagtttgaa gggaaaacag gattagcttt + 17041 tttaaaagat ggttttgttt atgtaaaaaa ttatgtgtta gacaatagat caattatatc + 17101 ctttgaacga gtaaaggaaa tagaagaatt ttcatttaaa cgttagtaat aagaaagagt + 17161 gtattatggt gaaaacatga aaaaaagaag tgtgttgtaa taacttcttt tttcatgttt + 17221 aaattatgaa tttctatatt ttaaataaag cataactgca cagactatta ttccaataac + 17281 taaaagtagc acagtaacaa tactttcatt tttatctatt tttctttcag gtctatagct + 17341 aggattaatt tgtttggcgt taaaatcagt aattccaaag tctaaagtag gaagtaaaaa + 17401 gagtaataaa ataatatata taattcccgc tataaggaaa ataggggcgc tagtgaaaag + 17461 tgaaataatg gtacctatga ttataggaat taaaatgtat aaaaaggatt tgataacaga + 17521 ttcttttctc attttaatta ccaaattttg ttattcatgt ttcccatatt tcctaaagga + 17581 acacaataac caccgctcat atttttccca cctgtagacc agccaccttt tttaaaataa + 17641 aaacctccag gacaagagct accaaattca gcttctcttg cactagcttc tgagctaaaa + 17701 ataaaaggtg ttaccatcgc gaaaatagtt aaagctaaac aaactttttt cttcatatac + 17761 gttccttctt tccaattatt atttaaaagc tatattgaat atagctttta aataataata + 17821 actgaatgaa aaaaaactgt ccaataatat atagaaattt ctgaaaaatc atttttaatg + 17881 taaattattt taataaaatt aataagaaag gaaaaaaata tgcaactaga atatccatta + 17941 acgaatcata tgaaaagaaa catggtttta actaaagaaa atcaagtgat tgcgtattat + 18001 cgtattcggt cagaaacagt tggcttaaca gattttgaga aaaaaaggaa aacaaagaaa + 18061 aaagtagctc gaacgttgaa acgtttacaa gagaatgagg gttttgaaat tgttctttta + 18121 cctgtgaatg cggatattcg agggaaaatg ggaggtatgc gccaattagt ggacaaagaa + 18181 aatcatagtg tagcggtgga taagttgatg aaaactgctc agtatcttga aaatgaaatt + 18241 ggcatggtct atgagtatat ttggttaatt ggcgtgcctt tagtaaagaa agagcggtca + 18301 attgatatta aagaaacatt ttctacggcg ttaaataatc tatctgaaaa agtggttaaa + 18361 ggacttggtt tagaagtagg agtagcggag gattgggaag aagcttataa agaccaagaa + 18421 caagaagtat atcagaattt gtcagaattg ttagtggaac gattgacaga agacgagttg + 18481 tattactacc aagcctatca atttttaaat aatattcaac atgagaaaaa agaacttttg + 18541 actagtcaga acttagataa tttactagcg aacaaaatca ctccttttcg aggtgggtta + 18601 gaattgtcta atgagtttgg gaaaagttat attgctcatt taccattggg ggattgtggg + 18661 gtcacgattg atggtaatca cttgctagaa ctggttcaaa aaatgtctta tcctgtttct + 18721 gtgaagttac aggctggttt tgctgaaaca aaaggccaat tagcgttatc aggacgttct + 18781 gctcgtgcga gaacacgaac aaagaatatc atggaagaag cacatttagc aggttcaaaa + 18841 caaaaaagaa aaattgttga ggggcaacat tcacttgatg atttagatca aaaaattgat + 18901 gatgatattc cgattattga ttggaaagca gtattagtga ttagtgggaa ttcaaaaaaa + 18961 caattacgtg agcgaaagaa aaatttgatg aatcgactag atagcttagg aattccgtta + 19021 atccgtgcaa cgtttgataa tgtctatctt tttcaagcaa cgttattagg caactttcaa + 19081 cgtttttcaa ccagtaattg gcaacatacg tcgacgttag aaacattttc agagttgaat + 19141 ttctttactt cgttacacgc aggtacaaag acagggtttt atttgggacg tgtggacgcc + 19201 acgttagaag aaaaagaaag tcggaaacaa attgtttctg gctctaaaaa tattgtctat + 19261 atgaatctat tgcttgcaaa caaacagaat attgaaggaa agaaaacaaa taatccccat + 19321 tggttggtat cgggagatac aggtaacggg aaatcagtat tttctaaatg gttatttttg + 19381 tattcttctc tattagatgt gaaagtgtta tacattgatc caaaaaaaga agttcgccaa + 19441 cagtttatgc gaacgattaa tgatccagaa tatcaaagaa aatacccact tgatgtggca + 19501 tttattaaga cgtttaattt tgtgacactt gatgttcgta aaaaagaaaa ccatggggta + 19561 cttgatccaa ttgtattatt tgatgaaaca gaagcaatcg caacggcaaa agctatgtta + 19621 aataatatta acgaggataa gtggaaaatg ccacataaga cggcgattaa tgaaacagta + 19681 gcagaagtgg tggctgaacg gaaagcaggc aaacaagttg gattttggca tgtaattgaa + 19741 cgtttaatta gtcattcaga gaaagacgtt catgaaatgg gtcgtttttt attatctacg + 19801 attaaaggat cgattttaga gttagccttt tctcatggtg aagtagaagg actttctttt + 19861 gagaagaaag tgaccatttt agaaattgaa gatttagatt tacccacaga tcggcacgat + 19921 gaattagacg agaatcaacg gttatctgtg actttaatgt ttgcattagg cacgttttgt + 19981 tcaaaatttg ggtcacgcaa tcggaaagaa gaaacagtca ctttctttga tgaagcgtgg + 20041 atttttcaat cgtcaccaga aggacaaaag attctaaaat cgatgaaacg gatcggacgt + 20101 tcgtttaata attttatggt attaattacg caatcggtca acgatgtgtt agataatggc + 20161 gatggtacag gctttggaac ggtggtttgt ttcgatgaag tggataatcg agaagggatt + 20221 ttacgttatt taaaattacc agtcaatgag attaatttaa aatgggtatc gaatatgatt + 20281 caaggacaat gtctcatgaa agatatgttt aaccaagtga atcgaatcgt gatagacgtt + 20341 atttttgaag aatggttaga actctttgaa acagttgatg atacagaagc gtccgttatg + 20401 gaaaacgatt atgtggcgag gtggtgagaa agtatgaaat atctcattga tagttataca + 20461 gcctatatgg tgaatggtgg tttattagat aaaggatcag aagtcgtcaa ttggttcttt + 20521 attgaattgc cttttttctt tttgcgtatg ggggcaatga ttttcttaat tatggagaat + 20581 gtcatgaatc agtcagatta ttttgttggt aaacaacaag aagcatacga ttattctttg + 20641 gatattttaa aaggatttgg tggaactggg attgtcaaag ggagtttgct tggattggca + 20701 attatcctaa gtgcctatta tctattgtat agcttttttt ctaatcggcg taatttcatg + 20761 aaaagtttgt tgcattattt tgcggtgttt gccttgttta tttgttggtt tggacaagta + 20821 aaaacaattg atggtaaaac gcaaaatggg gcaatttttt tgattagttc ggttagtgag + 20881 atgacaaaac aagtacaagg caagtttact tctaatgtaa actttggagg ggatacaagc + 20941 caagaagtag acgatggaaa gaaaaaagtg tatcaaagcc caatgtttga cgcaacggtt + 21001 ttacagacgt ttaactatgt caatagtggc tcgattgatg gcaaaatggc aaatgggaag + 21061 aagcttgatt atgataaact attagaaaag cctaacctga atgataaaga gaaaaaggat + 21121 tttagagaaa aaagaaatgc ttatattgaa cagcaggaga aagacaatcc ttatttttcg + 21181 caagatacct taaaaactat ggaaaaaagt tttgcggtat ggacgggtgc aacaaattta + 21241 tttattctag caattcctgt gttatatatt aatttaatgt tgtcgttgat acagctgtta + 21301 gttgtctttt taattcttat atttccagtt gttttgttgg cttccttttt tccacggtgt + 21361 caaatgttac tattcaagtt ctttcaaggt ttaattggtg cgttattcac gccaattatt + 21421 tatggtattt ttttatcggt gctgttttgg attaacaaac tgattgatgg ggcattttta + 21481 ggtgtcgcaa aaaaagtcag tggcagcttg cttgaattga tttcaggtag tacggtttat + 21541 atgatcgttt tatttgtagc agtggtagtg aagattgtag ttttacgaaa agtttggaaa + 21601 aataaatatg ccattcttgc ttacttttca aatgggcaag tgtcacagcc tgtgtttgaa + 21661 caaaaagtgg aacagttgtc tgaacgaacg aaagaagtag caactggtgg agcgcaagtt + 21721 gccctaggtg cttatacagg caatctgggc atggtcgcaa acggtgcagg taatattcta + 21781 ccgaatcaag ataaagccat tgaaatgggc caagaacatt ttattgatga taatggttca + 21841 tttactgggg taaaagcggg tttacagtct atttttaggc aaccagtgac agaagaagaa + 21901 caaggggagc tgaacgagga acagttttca gaagtggaaa cggaatcgat agatcatgag + 21961 ttaacagata ttgaagaaat aggacagaca gagttagaat ctgatagttt agataactct + 22021 attattgaat tagagcctat gacgagtgaa gatttagtag tggatgtcga taatatgagt + 22081 tctgatatag atgtggagct agcatcagta gaagaagttg gggaagagcc accaatgaac + 22141 gcagaggaat attcaggtta tgataatgtg attgtgacca actttgatga attggctatg + 22201 gcaagagaag aacgtgatta ttttgacggt ggcaaagaac aagagctagt cgagacaaat + 22261 ggagttgaat tggttaataa tatttatccg tttgttcaaa cagaagagta tctagctaat + 22321 cttgaacaga cagaagccca gttttttgga acagatgagt ttgagaaaga attagaacag + 22381 atagaggagt gggcataacc acttctcttt ttatgttagg tggtgcaaat gaaaaaaatc + 22441 tttttaggaa tccttggctt gtttagcttt ttgtttttat tgcctttttt gttatttttg + 22501 ggcaccatgt caacagaaat tggaaacaat acacaatttc aagcaacaac gccacaagaa + 22561 aaagtagcgc tagaggtatc caattttgtt accaaaaatg gtggatcgtt acaatttgcc + 22621 tctgcatggc taggaaatat ggaacatgag agcggattaa atcctgctag aatacaaagt + 22681 gatttaacgt ttaattcagc gtgggcgttt aatccttcga ccaatggtta tgcgttaggt + 22741 ttagcaatga tggacggtga gcgccgtgta aacttgttaa attttgccaa agaacaaaag + 22801 aaagattggc aggctgtccc agtacaactc gagtatatgt ggaatcatga cggctcagac + 22861 agtgccttgc tgaaacgtat gtcgaaaagc tctgatgtga atcaattagc tgtagatatt + 22921 ttggtacatt gggaacgtgc aggcactaaa aatgatccca acgaacaaat caaacgaaaa + 22981 acaagtgcga ataattggta taagagactg tctacaggtt ctatgggggc aggttcagcc + 23041 aatattggtg gtggcaaaat tgatgtgtta gaacaaatgt tagggcaaac agtcaatgga + 23101 ggtcagtgtt atggggggac ttcttattat gttgaaaaga tgggctttca atctttaatg + 23161 aatacagggc atatgtttgc cagtgaaatt ggcaatgatt atgcgtggga acaatctggc + 23221 tggcaggtaa tcaaaaatcc aaactattct gatgtcaaag caggtgatgt gattaatttc + 23281 gcaatgggcg gttatgcgac cagcgtttac ggtcatactg gaattgtagc tagtgttgaa + 23341 ggtaatggaa agcttgtttt atatgaacag aacgctgaaa aaggacaaat aattgctaaa + 23401 tattttcgac aatggggagc agaatatcct aacgtgacaa gtattgtaag gaagaaatag + 23461 aggagagatt tttgatgaaa aaagaagaaa tggacgttgt ttcattgcgg attataaagc + 23521 tttatttttt aggcattagg gaattaaatt tcccagatta taataaaagg tttcagcaaa + 23581 aagatatgga attgtttatt caactagctg atatgatgga aaatttacca aatcttgatg + 23641 aacaactaat ttatgaatta gaagagttga aagattattt attctatgtc aaaaccgaaa + 23701 agtattcttt aacagttcat gatatgtttt tggaaatgaa atccgagctt gaaaaaataa + 23761 tttagaagaa atgtagtgtc aaaaaagaga gggtgtagtg tcaaaaatgt ttagaaagaa + 23821 aaatggttta acaaatcggc aagtattagc gatttgtaca aaatttcagt gtaaaaagaa + 23881 tgagtttgtc gcccatcgat taagtgatgg gtatttagta gcagtaaata ataaagaata + 23941 ccgagtaaaa ttttcggaag gctttttttc aaaaattgta tatgcaaaag aagtgcaacg + 24001 gaaaggagct cggaaatcgt gaataagcta cctttactta ttttattgtt aggcggagtg + 24061 ttgcttgtta gtggctgtca aagccataag gaagaaaaca agtctagtaa agtatcgaca + 24121 gaagaaacga cagtgattga aacagtagca agggaacaat cgaaggaatc gtttacgagt + 24181 gaagcaacta aaaaacagac agaaacaacg aaattagaag aaccagatca tgtaaaactt + 24241 ctagaagctt atggaaatgc gtatgcgaac tttacaagta ttaatgatcg caatgaaaag + 24301 ctaaagcccc tcatgactga aaaatgtatc aaaaaaaatg gaattgatgt taaaactgga + 24361 gtagcgttag tttccgtagg aaaggttaca acgatttata aaaatgatca acatgaatat + 24421 gctttacttt tggattgtga acaaaatgga acgcagacac gagtgttact tttggctaag + 24481 gtgaagaaca ataaaatttc tgaaatgacc tataattcag ttaagcaaga gtattagaaa + 24541 ctcttgatat taatagattc catgctagat ttatggtaca attacacata ataaataaag + 24601 gagaaaacca atgaaaaaaa gtattgtaac gatgttattt ttgatgttgg ggatagttgt + 24661 atttactagt tgtgggtcta aaatggataa gacagcagaa gagtttaaga ataagattat + 24721 aagtcatgtc ggtgatcttt atgatgaaaa atatcaagaa aaagaattta gctttttaat + 24781 ttacaaagat aaggatacaa atgaatattt agctaaagct ttggttcctt atgaaggtga + 24841 gcctaatagt gtagaatcta agtattttta taatgtaaat aaagaattag aaactattga + 24901 accatttagt ggaggtagaa cgtttgatta tgctaagtct catggtaact atgaggtaat + 24961 atataaatca ggaaaattta aatagaattt gaacttaaga taaattaaat ttatgtttga + 25021 gagcagtgct tagagcattg ctcttttttg ttttaggagg gaaaacaatg ttaattgtaa + 25081 caaaagaaga cctaatgaat ttaggatatg gaaaatatca agcggaagat ataattagga + 25141 aagcaaaggc agtaatggtg tctaagggat acgcatatta cctaaataaa cgattaggaa + 25201 gagttcctgt tggagctgtc gaaagtgtat taggaataga actagaatct atagcaaaag + 25261 aggttgaaac ggttggttaa gtttaaaaaa gataagagag gaaagtatta ttttactgct + 25321 agtttgggtt ttgatgaagt aactggtaag agggttcaaa aaatgcgttc agggttttcc + 25381 acgattaaag aagcaaggga agcttatgcg gaaataatta ataatttcgg taaagaagca + 25441 tattcttcaa atagcacaat gctgtatgaa gaattttttt atactatttt tttgccatat + 25501 tataagggac gggttaaaga aagaacttat aataatagac ttagtatgat aaaaaatact + 25561 tttgcctatt tttttaaaat gaaattgaaa agtatagctc ctgttcatat acagaagtgg + 25621 cagaatgagc tgttggaaca gtatgaaaac acgtatgtaa gaaatattta tggactgttt + 25681 cagatgtctt tagatcgagc tgttgtgtta ggaatgattt cttcaaatcc agcaaagatc + 25741 gttggtaatg tgaaaaaaag taagaaagaa attgattttt ggacaaaaga agaatttgaa + 25801 aaagttgtta agacttttta tgtagaagat tattaccaaa atttttcttt tatttgtatc + 25861 tggttattat ttatgacagg catgagaata ggtgaggcta ctgctttaac ttggaaagat + 25921 gtgaatttag atagaagata tttaacagta aaaaaatcac tatattataa aaatgcgcaa + 25981 acttatgaat tagtgtctcc aaagactaga gccagtatta ggacgattta tttagatgaa + 26041 gatacagttc actatttaag agattggaaa aaaaggcaag atgatgttgg aggaattgaa + 26101 tttattttat cttataattc agttcctact caaaaacata cagtaagaca tattattaaa + 26161 cgtcatgcaa aattggcaga ggtacatgat attaggattc atgctttaag acattctcat + 26221 gcttctttat taataagtat gggtactaat gctttgttga taaaagaacg attgggtcat + 26281 gaagatgtgc aaacaacttt aggtacttat ggacacttat atccaagtag ctccactgaa + 26341 atagctaatg aattgaaggg aatagttaat gttgagttta ctaatcaaaa tatggcttcg + 26401 gaggttacta atcaatttac taaaggagta aaaaaataa +// diff -r 000000000000 -r 7c77b2896ac2 test-data/NC_004668_137848_164286_icescreen.gff --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/NC_004668_137848_164286_icescreen.gff Tue Jan 04 10:52:42 2022 +0000 @@ -0,0 +1,3 @@ +##gff-version 3 +##sequence-region NC_004668.1 1 26439 +NC_004668.1 ICEscreen CDS 1 1511 .+ 1 origid=WP_002359295.1;function=Coupling Protein;protein_id=WP_002359295.1;locus_tag=EF_RS00630;codon_start=1;color=6;Note=ICEscreen prediction%3A Coupling protein%3B BlastP result %28Annotation confidence%3A HIGH%29%3A Coupling protein TcpA %5BHit with AAO80014%3B Identity%3A100.00%25%3B E-value%3A0%3B Query coverage%3A100.00%25%5D%3B Hmmscan result%3A TcpA %5BHit with tcpA HMM profile%3B E-value%3A0%3B i-Evalue%3A0%5DNC_004668.1 ICEscreen CDS 3098 4380 .+ 1 origid=WP_002359299.1;function=Relaxase;protein_id=WP_002359299.1;locus_tag=EF_RS00650;codon_start=1;color=7;Note=ICEscreen prediction%3A Relaxase%3B BlastP result %28Annotation confidence%3A HIGH%29%3A Relaxase MOBT %28PF02486%29 %5BHit with AAO80018%3B Identity%3A99.77%25%3B E-value%3A0%3B Query coverage%3A95.53%25%5D%3B Hmmscan result%3A MOBT %5BHit with T4SS_MOBT HMM profile%3B E-value%3A0%3B i-Evalue%3A0%5DNC_004668.1 ICEscreen CDS 17920 20426 .+ 1 origid=WP_002359320.1;function=VirB4;protein_id=WP_002359320.1;locus_tag=EF_RS00720;codon_start=1;color=184 134 11;Note=ICEscreen prediction%3A VirB4%3B BlastP result %28Annotation confidence%3A HIGH%29%3A VirB4 %5BHit with CAE52368%3B Identity%3A41.15%25%3B E-value%3A0%3B Query coverage%3A99.76%25%5D%3B Hmmscan result%3A VirB4 %5BHit with T4SS_virb4 HMM profile%3B E-value%3A0%3B i-Evalue%3A0%5DNC_004668.1 ICEscreen CDS 25273 26438 .+ 1 origid=WP_002392915.1;function=Tyrosine Integrase;protein_id=WP_002392915.1;locus_tag=EF_RS00760;codon_start=1;color=8;Note=ICEscreen prediction%3A Tyrosine integrase%3B BlastP result %28Annotation confidence%3A HIGH%29%3A Tyrosine integrase %5BHit with AAO80040%3B Identity%3A100.00%25%3B E-value%3A0%3B Query coverage%3A100.00%25%5D%3B Hmmscan result%3A Tyrosine integrase %5BHit with Phage_integrase HMM profile%3B E-value%3A0%3B i-Evalue%3A0%5DNC_004668.1 ICEscreen mobile_element 1 26439 .+ . mobile_element_type=other%3A integrative and conjugative element;Note=ICEscreen prediction%3A Putative ICE %28one integrase - Tyr%29 %5BElement structure%3A Single%3B ICE superfamily%3A Tn916%3B ICE family%3A ICESt3%3B Relaxase family%3A MOBT%3B Coupling protein family%3A TcpA%5D %28ICEscreen ID%3A 2%29;color=15 \ No newline at end of file diff -r 000000000000 -r 7c77b2896ac2 test-data/genbank/NC_004668_137848_164286.gb --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/genbank/NC_004668_137848_164286.gb Tue Jan 04 10:52:42 2022 +0000 @@ -0,0 +1,1112 @@ +LOCUS NC_004668 26439 bp DNA linear CON 02-APR-2021 +DEFINITION Enterococcus faecalis V583, complete sequence. +ACCESSION NC_004668 REGION: 137848..164286 +VERSION NC_004668.1 +DBLINK BioProject: PRJNA224116 + BioSample: SAMN02603978 + Assembly: GCF_000007785.1 +KEYWORDS RefSeq. +SOURCE Enterococcus faecalis V583 + ORGANISM Enterococcus faecalis V583 + Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; + Enterococcus. +REFERENCE 1 (bases 1 to 26439) + AUTHORS Paulsen,I.T., Banerjei,L., Myers,G.S., Nelson,K.E., Seshadri,R., + Read,T.D., Fouts,D.E., Eisen,J.A., Gill,S.R., Heidelberg,J.F., + Tettelin,H., Dodson,R.J., Umayam,L., Brinkac,L., Beanan,M., + Daugherty,S., DeBoy,R.T., Durkin,S., Kolonay,J., Madupu,R., + Nelson,W., Vamathevan,J., Tran,B., Upton,J., Hansen,T., Shetty,J., + Khouri,H., Utterback,T., Radune,D., Ketchum,K.A., Dougherty,B.A. + and Fraser,C.M. + TITLE Role of mobile DNA in the evolution of vancomycin-resistant + Enterococcus faecalis + JOURNAL Science 299 (5615), 2071-2074 (2003) + PUBMED 12663927 +REFERENCE 2 (bases 1 to 26439) + AUTHORS Paulsen,I., Banerjei,L., Myers,G., Nelson,K., Seshadri,R., Read,T., + Fouts,D., Eisen,J., Gill,S., Heidelberg,J., Tettelin,H., Dodson,R., + Umayam,L., Brinkac,L., Beanan,M., Daugherty,S., DeBoy,R., + Durkin,A., Kolonay,J., Madupu,R., Nelson,W., Vamathevan,J., + Tran,B., Upton,J., Hansen,T., Shetty,J., Khouri,H., Utterback,T., + Radune,D., Ketchum,K., Dougherty,B. and Fraser,C. + TITLE Direct Submission + JOURNAL Submitted (03-FEB-2003) The Institute for Genomic Research, 9712 + Medical Center Dr, Rockville, MD 20850, USA +COMMENT REFSEQ INFORMATION: The reference sequence is identical to + AE016830.1. + The annotation was added by the NCBI Prokaryotic Genome Annotation + Pipeline (PGAP). Information about PGAP can be found here: + https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ + + ##Genome-Annotation-Data-START## + Annotation Provider :: NCBI RefSeq + Annotation Date :: 04/02/2021 02:16:18 + Annotation Pipeline :: NCBI Prokaryotic Genome + Annotation Pipeline (PGAP) + Annotation Method :: Best-placed reference protein + set; GeneMarkS-2+ + Annotation Software revision :: 5.1 + Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; + repeat_region + Genes (total) :: 3,323 + CDSs (total) :: 3,240 + Genes (coding) :: 3,166 + CDSs (with protein) :: 3,166 + Genes (RNA) :: 83 + rRNAs :: 4, 4, 4 (5S, 16S, 23S) + complete rRNAs :: 4, 4, 4 (5S, 16S, 23S) + tRNAs :: 67 + ncRNAs :: 4 + Pseudo Genes (total) :: 74 + CDSs (without protein) :: 74 + Pseudo Genes (ambiguous residues) :: 0 of 74 + Pseudo Genes (frameshifted) :: 39 of 74 + Pseudo Genes (incomplete) :: 28 of 74 + Pseudo Genes (internal stop) :: 13 of 74 + Pseudo Genes (multiple problems) :: 6 of 74 + ##Genome-Annotation-Data-END## + COMPLETENESS: full length. +FEATURES Location/Qualifiers + source 1..26439 + /organism="Enterococcus faecalis V583" + /mol_type="genomic DNA" + /strain="V583" + /db_xref="taxon:226185" + gene 1..1512 + /locus_tag="EF_RS00630" + /old_locus_tag="EF0139" + /old_locus_tag="EF_0139" + CDS 1..1512 + /locus_tag="EF_RS00630" + /old_locus_tag="EF0139" + /old_locus_tag="EF_0139" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359295.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="cell division protein FtsK" + /protein_id="WP_002359295.1" + /translation="MLKKLFRYRGRRIRYSSRNLLALYRVLFFMPILACLGYFVGYKW + IYPLYLSNPPDWKIYIVPALIIVGISIGAIVLITLLIKASIINSGYFSKVEQRQVLAH + MIIDNGYYTKKQVKSSDGKTKEKIKFPKIYYKSAKNSIFVSFETAGNKFQEKFETIGG + FLETTFHADNLNKIDEKGFVTYELATDVYNKRIWIKDMQADEGKVQLMKGLYWHFDKD + PHLLLGGGTGGGKTFTILSLIYALCRVGEVEICDPKNSDLMALGKLPLFAGKVHTGKK + DITQCLENTVELMETRFKTMNNSSRYKMGKNYAYYGLKPKFVFIDEFAAFKAELANDY + STDGEVDEYLTQLILKARQAGIFFIVAMQRPDGEFLKTALRDQFMFRMSVGRLSETGI + LMIFGDENKNKKFKYVEKIDGQKVYGRGYVAQGGGTAREFYSPQVPQDFDFIEEFIKI + SKELGYEDVPKEVQEEVSQKISKHIDKEALAEIDEEFQAEKNQLSELSEKYSA" + gene 1552..2085 + /locus_tag="EF_RS00635" + /old_locus_tag="EF0140" + /old_locus_tag="EF_0140" + CDS 1552..2085 + /locus_tag="EF_RS00635" + /old_locus_tag="EF0140" + /old_locus_tag="EF_0140" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359296.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359296.1" + /translation="MDSNEIIKRVRERVYREVKKKYTRDDLDTRIQDVLYYRSETYMK + LVSFANGKRIKKLADPRKFEKFMDTKGVKIVAEVLDGLNNQPKMQAMEYEQKVLTKVR + QWYQKKNHPELVDLEEEAFEQLVEKNIIYKKMKKRLYEEQDNQGFVYSDNFDMQLIRD + SCDIEEALYLDITLGDY" + gene 2106..2393 + /locus_tag="EF_RS00640" + /old_locus_tag="EF0141" + /old_locus_tag="EF_0141" + CDS 2106..2393 + /locus_tag="EF_RS00640" + /old_locus_tag="EF0141" + /old_locus_tag="EF_0141" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359297.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359297.1" + /translation="MKFKSRVQMLNYVIGKKPDIMKTVDRRAEKEGVTDDNRLLDYKL + LVISVLDAEAVYDEDRPIEEKKFSTSQEVDKYLETRFKEHMDSAWKEMFNK" + gene 2403..2807 + /locus_tag="EF_RS00645" + /old_locus_tag="EF0142" + /old_locus_tag="EF_0142" + CDS 2403..2807 + /locus_tag="EF_RS00645" + /old_locus_tag="EF0142" + /old_locus_tag="EF_0142" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002414864.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002414864.1" + /translation="MYSFDQVILELDNAIALKTLKNWANRIEKMTDTRFERRYAKNSA + GHSYSYKVFSIKDIENFQELLRLRENNVPLNEAINEVFMSDENKATHINKQEIEDLKR + DMRQLLKVSQSILAENASLKERIGALERGTAE" + gene 3098..4381 + /locus_tag="EF_RS00650" + /old_locus_tag="EF0143" + /old_locus_tag="EF_0143" + CDS 3098..4381 + /locus_tag="EF_RS00650" + /old_locus_tag="EF0143" + /old_locus_tag="EF_0143" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_016627242.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="replication initiation factor domain-containing + protein" + /protein_id="WP_002359299.1" + /translation="MRGVDLKRYRKELKLKQQELASKLGIERSLISKIESGKRVISKE + LEQKIINVLNLDGGHASVEAKIDFLRIRFKTLDVRTVIEKLLHMDMNWFTHESRGFYH + YTETFSYSSIRIFRNPENVNMGIMLDLSGEGCRQLEEIFEEDNNRSWTEFFRSLYDDD + IFGQGILVDTKITRIDIALDELIVKGQENFDLYVLKEKMEQGLVDTTFKNFDFSGGFV + YENKKMVNKGLSLYFGSRQSPLYFNFYQKDYELARKESMSVEEAREKHEIKNRYEIRL + SDEKAFLFVEYFLSSGESLDWLVKEIINQSLTVYDIEDDMKVYCKSWHDVVDKLEGLK + LSVQGEKPSIEKTLRWLSNYLAPSLKMIKEIDNLLGTNELMERIDLAELKEKHEEIIE + MVSVDAKDLLFTNRDNKSVRSYMEREFELEEMYPF" + gene 4399..5013 + /locus_tag="EF_RS00655" + /old_locus_tag="EF0144" + /old_locus_tag="EF_0144" + CDS 4399..5013 + /locus_tag="EF_RS00655" + /old_locus_tag="EF0144" + /old_locus_tag="EF_0144" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002393276.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002393276.1" + /translation="MDKELIKKNIKLMDATIEKMIHVMSSIHKLQLSVKELEDSNELT + VSYVKRECTKLEERVKELRPSISRCVFMYREYVEEFETKLRDRIVTEKFFRIKRFYGK + EVLAFKEFQEKYQNYIPKDIIDIKKQVRQKLEEVGYEIDGAFEGDFTSWVGVYARPKD + KPTYLDPANAEEVVLQEKYSVNGFKQDFSEWFEFEIKDNVVYGI" + gene 5111..5416 + /locus_tag="EF_RS00660" + /old_locus_tag="EF0145" + /old_locus_tag="EF_0145" + CDS 5111..5416 + /locus_tag="EF_RS00660" + /old_locus_tag="EF0145" + /old_locus_tag="EF_0145" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002365956.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_010782977.1" + /translation="MTKKLIKIWRKEMLALMNEDADWYRNEDTERFKRIQELARKIET + ANTRQFSSHISKERFEAYQKMGLQFKEIAEEFHITTTALQQWRKDNGYPIYNKNNRK" + gene 5427..8132 + /gene="sea1" + /locus_tag="EF_RS00665" + /old_locus_tag="EF0146" + /old_locus_tag="EF_0146" + /gene_synonym="prgA" + CDS 5427..8132 + /gene="sea1" + /locus_tag="EF_RS00665" + /old_locus_tag="EF0146" + /old_locus_tag="EF_0146" + /gene_synonym="prgA" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_011109619.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="surface exclusion protein SEA1/PrgA" + /protein_id="WP_010784266.1" + /translation="MKKIASAGLSILVATGVAGIGGNEVQAAEQAQPKTPENSSTEQP + AVKATETTEQAITEKQQQVTEKQAIVDQKQQVADTAKKEKDTIDQSVKDQQAVVDQNK + GALDQSQQAVTDQQAVVDEAKKVVDEATPSAIEKAKNQVATDTQAVDDQQKVVDQAQA + DVNQQQAVVEEKAKETNAAKVQNDKDQQAVTAAKQEQAKLEELAKNAEVEKAKAEKEQ + AAKEAELANKQKEEAKAKDQKTKDDQAVADQQTVVTTSQEKVADAKADTAAKQADLTA + KENALKDKQTATKQVQNTLDKSKEELKGHKGINLPANFTPDYYKKLSEQEKQAMEKEA + LALNKVFPENQADVAKATEMINVKNPTGKQKQQMSDYVVGLINDVREKLGLQKLKISN + QAMRFAWDVAKYDNPKEFDHDVNAINRAAKENGFKEYPGQNFYENLSMGYFETINGTI + SQLEFEKAARKTIADMLFDDESSAYSHIDSLLKGDTTNMAVSISGDLNDISAKIHIIS + YNQSKLVEANTYEEGTAPVFKSKETLQKEVATNQEKLATAQQAESDAQQAKSASQQVL + NTAKTTQATAEKELSVHKATLASLQAVATKSTTNYEEKVRQTATAEKSLQQTKDQLAT + INELIQNRAAVLEKAKTNVSEAQAIEQTSAKVLKEKQAAQKAEENTLNSLKEVLNLAK + ENLNQKQVAFKTSTRSLSRLENAQPTYEKALNELNKAEAAAVQAQEAYENSLKSLEEL + KEQQAVATLAYTQAQEDLSNAKLELQQYQGVLRELEAQQAEQQRQEALQEQVAKEQQR + LEREAKQSQTLVASATSADKTPGLQQLSFSKQKEQPKAQALTHSESRKTKQVAKAPDS + LPHTGEKNNKWLAIAGLIFALLGAAGIISFISRNEKKVKNIFKIK" + gene 8603..12520 + /locus_tag="EF_RS00670" + /old_locus_tag="EF0149" + /old_locus_tag="EF_0149" + CDS 8603..12520 + /locus_tag="EF_RS00670" + /old_locus_tag="EF0149" + /old_locus_tag="EF_0149" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002395058.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="LPXTG-anchored aggregation substance" + /protein_id="WP_011109430.1" + /translation="MNQQTEVKKRFKMYKAKKHWIVAPILFLGVLGAVGLATDNVQAA + ELDTQPGTTTVQPDNPNPQSRNETLKTAVSEEAALQKDTTSQPTTAEEVVPKGIAAEQ + SSATSNDTTNVQQPTAEAEKSAQEQPVVSPETTIEPLGQPTEVAPAENDANKSTSIPK + EFETPDVDKAVDEAKKDPNITVVEKPTEDLGNVSSKDLAAKEKEVDQLQKEQAQKIAQ + QAAELKAKNEKIAKENAEIAAKNKAEKERYEKEVAEYNKHKNDKGYVNEAISKDLVFD + SSIVTKDTKIDKITGGKFIKASDFNKVNQGQSKDIFTKLSKDMNGKATGNFQSSKVAA + VEFGPKGGYAVLLEKNKPVNVTYTGLNASYLDRKITKAEFIYELQSAPSQSGTLNAVF + SNDPIITAFVGTKNANGKDVKVRLTIKLYDANGKEVLPDKDHAFAYALSSLNSSLGTN + YSVEHAEFVSDFGSKNEFKYINGSYVKKQADGKFYSTEDLDYGTGPSGLKNSDWDAVG + HKNAYYGSGVGLAREGGRISFSFGMTTKGKVNLSGAQWFAFSTNLNAKSIRPYQKKGN + PKEPEKATIEFNRYKANVVPVLVPNKEVTDGQKNINDLNVKRGDSLQYIVTGDTTELA + KVDPKTVTKQGIRDTFDAEKVTIDLSKVKVYQADASLNEKDLKAVAAAINSGKAIDVT + ASYVLNLDQNTVTAMMKTNADGSVVLAMGYKYLLVLPFVVKNVEGDFENTAVQLTNDG + ETVTNTVINHVPVSNPSKDVKADKNGTVGSVSLHDKDIPLQTKIYYEVKSSERPANYG + GITEEWGMNDVLDTTHDRFTGKWHAITNYDLKVGDKTLKAGTDISAYILLENKDNKDL + TFTMNQALLAALNEGSNKVGKQAWSVYLEVERIKTGDVENTQTENYNKELVRSNTVVT + HTPDDPKPTKAVHNKKGEDINHGKVARGDVLSYEMTWDLKGYDKDFAFDTVDLATGVS + FFDDYDETKVTPIKDLLRVKDSKGADITNQFTISWDDAKGTVTISAKDPQAFILAYGG + QELRVTLPTKVKANVSGDVYNSAEQNTFGQRIKTNTVVNHIPKVNPKKDVVIKVGDKQ + SQNGATIKLGEKFFYEFTSSDIPAEYAGVVEEWSISDKLDVKHDKFSGQWSVFANSNF + VLADGTKVNKGDDISKLFTMTFEQGVVKITASQAFLDAMNLKENKHVAHSWKAFIGVE + RIAAGDVYNTIEESFNNEKIKTNTVVTHTPEKPQTPPEKTVIVPPTPKTPQAPVEPLV + VEKASVAPELPHTGEKENTLLSVLGAGMLVGLAWFGLKKREVK" + gene complement(12713..13570) + /locus_tag="EF_RS00675" + /old_locus_tag="EF0150" + /old_locus_tag="EF_0150" + CDS complement(12713..13570) + /locus_tag="EF_RS00675" + /old_locus_tag="EF0150" + /old_locus_tag="EF_0150" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359308.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359308.1" + /translation="MNIDFFNQLIKPVGILLPIVIGFFTIYSSIYGYVISDSFKKNLF + SSPTEIGLYKIFKGFINLISSLLLSSGIFAYALITTDTAFTDFVSDINNLTKEGKAYL + TYFIIYFIVFIFSIAKLPDIYRTLIPSNSRSKHSNFYVLSQELSLNDIPCNTKVFLVS + MINKDTVLLTYYLNKEFVRLIYSLNEIQKIKIFYHKQLSFFQKIKEFNESITKETYYF + AFLPTIFIMLPSIIMSFISKSMTNIYFGATIAAISELIILSPSLIKYIKIAYKKIYLT + IKNQNKPKK" + gene 13629..14183 + /locus_tag="EF_RS00680" + /old_locus_tag="EF0151" + /old_locus_tag="EF_0151" + CDS 13629..14183 + /locus_tag="EF_RS00680" + /old_locus_tag="EF0151" + /old_locus_tag="EF_0151" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359309.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359309.1" + /translation="MLIKLGTELTKEEYVTRYMRNFQKLLLLGDRPKVLTNREEQLLQ + YEKELCVLFYEQFIKKHHRAPDEATLDDQVKANFIERSKIFARSPLVMDEGNFTQAHI + GQLKRLRELRMEDYLPDNYTHILQREEELARNYFRKHDDYPFGYECLCISRSREVVNQ + GLEKLLEGFYDSYQVYYRRYRKNG" + gene 14226..14669 + /locus_tag="EF_RS00685" + /old_locus_tag="EF0152" + /old_locus_tag="EF_0152" + CDS 14226..14669 + /locus_tag="EF_RS00685" + /old_locus_tag="EF0152" + /old_locus_tag="EF_0152" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359311.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359311.1" + /translation="MAEESKYPPVKEKSVFYQRYSRVSTISMLMDLGFSVSEAQIDNE + DIVYMEQYKEFLLNKGIVEEKVPFKREFVAEYPLSKMRRLFVEGYYLRKPSGEKMYGS + YYYDFYFKEYSGMGIYCTKATKSLFLHVFEQRIRKIKKLKHRLEE" + gene 14672..15520 + /locus_tag="EF_RS00690" + /old_locus_tag="EF0153" + /old_locus_tag="EF_0153" + CDS 14672..15520 + /locus_tag="EF_RS00690" + /old_locus_tag="EF0153" + /old_locus_tag="EF_0153" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_011109431.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="LPXTG cell wall anchor domain-containing + protein" + /protein_id="WP_011109431.1" + /translation="MKKMIIIALFSTSLLAGGSSVSAYAQESEGNLGETTGSVLPDEP + NVPTDPITPSEPEQPTEPSTPEQPSEPSTPTEPSEPSKPTDPSLPDEPSVPTEPTTPS + KPEQPTEPTTPSVPEQPTEPSVPEKPVEPNKPTEPEKPVPVVPEKPVVPQQPEQPTDV + VVKPNGEIATGESTQQPTVPIETNNLSEVTHVPTVTTPIETASGEAIVAVDKGVPLTQ + TADGLKPIKSEYKVLPSGNVQVKSADGKMKVLPYTGEKMGIIGSIAGVCLTVLSGILI + YKKRKV" + gene 15531..16520 + /locus_tag="EF_RS00695" + /old_locus_tag="EF0154" + /old_locus_tag="EF_0154" + CDS 15531..16520 + /locus_tag="EF_RS00695" + /old_locus_tag="EF0154" + /old_locus_tag="EF_0154" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002370321.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="conjugal transfer protein" + /protein_id="WP_002359315.1" + /translation="MKFLKKIQTKEKSKKSPRVRLLSQKKANRLVLIVGCALFGLSLI + GAIRANVMAGNVIRLSQQLETINKDVKKIKEKKRVYDSSALSFYVRNFVNEYINFNGK + ATDEEKKARDEKLASYFSGDLALDKGTDNAAKDLIRKLLQVSVTRVEESEELLLVHAS + ISYKVQQKGQDLLDTQEIVLPIQEKNGLFSIVGRPYFLAMNVPKGKQEPLQSVKEPLD + VEKKERQGIEKFLQLFFEKYAKGNTEELSVLMKEPVKTSGQATVSSIEEKELRFFETK + QKGVTGVQVSVNFKDKAINFLYTEDFSLWLTKTENSYFVQELKHYYTEKAGDK" + gene 16520..16744 + /locus_tag="EF_RS00700" + /old_locus_tag="EF0155" + /old_locus_tag="EF_0155" + CDS 16520..16744 + /locus_tag="EF_RS00700" + /old_locus_tag="EF0155" + /old_locus_tag="EF_0155" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002370320.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359317.1" + /translation="MDTSKLSQFMSSGLITLIAFGVIILILKHWKEAAWLKIGSVIVI + ALILNDFATNNGQNIFKVVKWVLGLFGIQL" + gene 16757..17146 + /locus_tag="EF_RS00705" + /old_locus_tag="EF0156" + /old_locus_tag="EF_0156" + CDS 16757..17146 + /locus_tag="EF_RS00705" + /old_locus_tag="EF0156" + /old_locus_tag="EF_0156" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_016626678.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="conjugal transfer protein" + /protein_id="WP_002359318.1" + /translation="MKHFDYSRGLKAPYSLQVIKSPKGKIVWYFAQPLSLAYLVMLFL + GIVLTGIFWKFVPLPLIFGINLNLMIMLYFPNKVARWYTETEFEGKTGLAFLKDGFVY + VKNYVLDNRSIISFERVKEIEEFSFKR" + gene complement(17224..17532) + /locus_tag="EF_RS00710" + CDS complement(17224..17532) + /locus_tag="EF_RS00710" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002386531.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002386531.1" + /translation="MRKESVIKSFLYILIPIIIGTIISLFTSAPIFLIAGIIYIILLL + FLLPTLDFGITDFNAKQINPSYRPERKIDKNESIVTVLLLVIGIIVCAVMLYLKYRNS + " + gene complement(17538..17756) + /locus_tag="EF_RS00715" + /old_locus_tag="EF0157" + /old_locus_tag="EF_0157" + CDS complement(17538..17756) + /locus_tag="EF_RS00715" + /old_locus_tag="EF0157" + /old_locus_tag="EF_0157" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359319.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359319.1" + /translation="MKKKVCLALTIFAMVTPFIFSSEASAREAEFGSSCPGGFYFKKG + GWSTGGKNMSGGYCVPLGNMGNMNNKIW" + gene 17920..20427 + /locus_tag="EF_RS00720" + /old_locus_tag="EF0158" + /old_locus_tag="EF_0158" + CDS 17920..20427 + /locus_tag="EF_RS00720" + /old_locus_tag="EF0158" + /old_locus_tag="EF_0158" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359320.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="ATP-binding protein" + /protein_id="WP_002359320.1" + /translation="MQLEYPLTNHMKRNMVLTKENQVIAYYRIRSETVGLTDFEKKRK + TKKKVARTLKRLQENEGFEIVLLPVNADIRGKMGGMRQLVDKENHSVAVDKLMKTAQY + LENEIGMVYEYIWLIGVPLVKKERSIDIKETFSTALNNLSEKVVKGLGLEVGVAEDWE + EAYKDQEQEVYQNLSELLVERLTEDELYYYQAYQFLNNIQHEKKELLTSQNLDNLLAN + KITPFRGGLELSNEFGKSYIAHLPLGDCGVTIDGNHLLELVQKMSYPVSVKLQAGFAE + TKGQLALSGRSARARTRTKNIMEEAHLAGSKQKRKIVEGQHSLDDLDQKIDDDIPIID + WKAVLVISGNSKKQLRERKKNLMNRLDSLGIPLIRATFDNVYLFQATLLGNFQRFSTS + NWQHTSTLETFSELNFFTSLHAGTKTGFYLGRVDATLEEKESRKQIVSGSKNIVYMNL + LLANKQNIEGKKTNNPHWLVSGDTGNGKSVFSKWLFLYSSLLDVKVLYIDPKKEVRQQ + FMRTINDPEYQRKYPLDVAFIKTFNFVTLDVRKKENHGVLDPIVLFDETEAIATAKAM + LNNINEDKWKMPHKTAINETVAEVVAERKAGKQVGFWHVIERLISHSEKDVHEMGRFL + LSTIKGSILELAFSHGEVEGLSFEKKVTILEIEDLDLPTDRHDELDENQRLSVTLMFA + LGTFCSKFGSRNRKEETVTFFDEAWIFQSSPEGQKILKSMKRIGRSFNNFMVLITQSV + NDVLDNGDGTGFGTVVCFDEVDNREGILRYLKLPVNEINLKWVSNMIQGQCLMKDMFN + QVNRIVIDVIFEEWLELFETVDDTEASVMENDYVARW" + gene 20434..22398 + /locus_tag="EF_RS00725" + /old_locus_tag="EF0159" + /old_locus_tag="EF_0159" + CDS 20434..22398 + /locus_tag="EF_RS00725" + /old_locus_tag="EF0159" + /old_locus_tag="EF_0159" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002403502.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359321.1" + /translation="MKYLIDSYTAYMVNGGLLDKGSEVVNWFFIELPFFFLRMGAMIF + LIMENVMNQSDYFVGKQQEAYDYSLDILKGFGGTGIVKGSLLGLAIILSAYYLLYSFF + SNRRNFMKSLLHYFAVFALFICWFGQVKTIDGKTQNGAIFLISSVSEMTKQVQGKFTS + NVNFGGDTSQEVDDGKKKVYQSPMFDATVLQTFNYVNSGSIDGKMANGKKLDYDKLLE + KPNLNDKEKKDFREKRNAYIEQQEKDNPYFSQDTLKTMEKSFAVWTGATNLFILAIPV + LYINLMLSLIQLLVVFLILIFPVVLLASFFPRCQMLLFKFFQGLIGALFTPIIYGIFL + SVLFWINKLIDGAFLGVAKKVSGSLLELISGSTVYMIVLFVAVVVKIVVLRKVWKNKY + AILAYFSNGQVSQPVFEQKVEQLSERTKEVATGGAQVALGAYTGNLGMVANGAGNILP + NQDKAIEMGQEHFIDDNGSFTGVKAGLQSIFRQPVTEEEQGELNEEQFSEVETESIDH + ELTDIEEIGQTELESDSLDNSIIELEPMTSEDLVVDVDNMSSDIDVELASVEEVGEEP + PMNAEEYSGYDNVIVTNFDELAMAREERDYFDGGKEQELVETNGVELVNNIYPFVQTE + EYLANLEQTEAQFFGTDEFEKELEQIEEWA" + gene 22429..23460 + /locus_tag="EF_RS00730" + /old_locus_tag="EF0160" + /old_locus_tag="EF_0160" + CDS 22429..23460 + /locus_tag="EF_RS00730" + /old_locus_tag="EF0160" + /old_locus_tag="EF_0160" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002386530.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="phage tail tip lysozyme" + /protein_id="WP_010775499.1" + /translation="MKKIFLGILGLFSFLFLLPFLLFLGTMSTEIGNNTQFQATTPQE + KVALEVSNFVTKNGGSLQFASAWLGNMEHESGLNPARIQSDLTFNSAWAFNPSTNGYA + LGLAMMDGERRVNLLNFAKEQKKDWQAVPVQLEYMWNHDGSDSALLKRMSKSSDVNQL + AVDILVHWERAGTKNDPNEQIKRKTSANNWYKRLSTGSMGAGSANIGGGKIDVLEQML + GQTVNGGQCYGGTSYYVEKMGFQSLMNTGHMFASEIGNDYAWEQSGWQVIKNPNYSDV + KAGDVINFAMGGYATSVYGHTGIVASVEGNGKLVLYEQNAEKGQIIAKYFRQWGAEYP + NVTSIVRKK" + gene 23475..23765 + /locus_tag="EF_RS00735" + /old_locus_tag="EF0161" + /old_locus_tag="EF_0161" + CDS 23475..23765 + /locus_tag="EF_RS00735" + /old_locus_tag="EF0161" + /old_locus_tag="EF_0161" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359323.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_002359323.1" + /translation="MKKEEMDVVSLRIIKLYFLGIRELNFPDYNKRFQQKDMELFIQL + ADMMENLPNLDEQLIYELEELKDYLFYVKTEKYSLTVHDMFLEMKSELEKII" + gene 23807..24022 + /locus_tag="EF_RS00740" + /old_locus_tag="EF0162" + /old_locus_tag="EF_0162" + CDS 23807..24022 + /locus_tag="EF_RS00740" + /old_locus_tag="EF0162" + /old_locus_tag="EF_0162" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002393223.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_025186164.1" + /translation="MFRKKNGLTNRQVLAICTKFQCKKNEFVAHRLSDGYLVAVNNKE + YRVKFSEGFFSKIVYAKEVQRKGARKS" + gene 24019..24537 + /locus_tag="EF_RS00745" + /old_locus_tag="EF0163" + /old_locus_tag="EF_0163" + CDS 24019..24537 + /locus_tag="EF_RS00745" + /old_locus_tag="EF0163" + /old_locus_tag="EF_0163" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002370311.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="lipoprotein" + /protein_id="WP_002359325.1" + /translation="MNKLPLLILLLGGVLLVSGCQSHKEENKSSKVSTEETTVIETVA + REQSKESFTSEATKKQTETTKLEEPDHVKLLEAYGNAYANFTSINDRNEKLKPLMTEK + CIKKNGIDVKTGVALVSVGKVTTIYKNDQHEYALLLDCEQNGTQTRVLLLAKVKNNKI + SEMTYNSVKQEY" + gene 24611..24985 + /locus_tag="EF_RS00750" + /old_locus_tag="EF0164" + /old_locus_tag="EF_0164" + CDS 24611..24985 + /locus_tag="EF_RS00750" + /old_locus_tag="EF0164" + /old_locus_tag="EF_0164" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002359326.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="lipoprotein" + /protein_id="WP_002359326.1" + /translation="MKKSIVTMLFLMLGIVVFTSCGSKMDKTAEEFKNKIISHVGDLY + DEKYQEKEFSFLIYKDKDTNEYLAKALVPYEGEPNSVESKYFYNVNKELETIEPFSGG + RTFDYAKSHGNYEVIYKSGKFK" + gene 25068..25280 + /locus_tag="EF_RS00755" + /old_locus_tag="EF0165" + /old_locus_tag="EF_0165" + CDS 25068..25280 + /locus_tag="EF_RS00755" + /old_locus_tag="EF0165" + /old_locus_tag="EF_0165" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_016623907.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="DUF3173 domain-containing protein" + /protein_id="WP_002359328.1" + /translation="MLIVTKEDLMNLGYGKYQAEDIIRKAKAVMVSKGYAYYLNKRLG + RVPVGAVESVLGIELESIAKEVETVG" + gene 25273..26439 + /locus_tag="EF_RS00760" + /old_locus_tag="EF0166" + /old_locus_tag="EF_0166" + CDS 25273..26439 + /locus_tag="EF_RS00760" + /old_locus_tag="EF0166" + /old_locus_tag="EF_0166" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_002392915.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="site-specific integrase" + /protein_id="WP_002392915.1" + /translation="MVKFKKDKRGKYYFTASLGFDEVTGKRVQKMRSGFSTIKEAREA + YAEIINNFGKEAYSSNSTMLYEEFFYTIFLPYYKGRVKERTYNNRLSMIKNTFAYFFK + MKLKSIAPVHIQKWQNELLEQYENTYVRNIYGLFQMSLDRAVVLGMISSNPAKIVGNV + KKSKKEIDFWTKEEFEKVVKTFYVEDYYQNFSFICIWLLFMTGMRIGEATALTWKDVN + LDRRYLTVKKSLYYKNAQTYELVSPKTRASIRTIYLDEDTVHYLRDWKKRQDDVGGIE + FILSYNSVPTQKHTVRHIIKRHAKLAEVHDIRIHALRHSHASLLISMGTNALLIKERL + GHEDVQTTLGTYGHLYPSSSTEIANELKGIVNVEFTNQNMASEVTNQFTKGVKK" +ORIGIN + 1 atgttaaaaa aattatttag atatagagga aggcgtattc gttattcttc aagaaacctg + 61 ttggcactct atcgagtgtt attttttatg ccaattttag cttgtttagg gtattttgtt + 121 ggctataaat ggatttatcc tttatatctt tcaaatccgc ctgattggaa aatatacatt + 181 gtgccagcgt tgataattgt aggtatttct ataggtgcaa ttgtgctaat tacattgctt + 241 attaaagctt caattattaa ttcggggtat ttttctaaag tggaacaacg tcaagtatta + 301 gcacatatga ttattgacaa cgggtattac acaaaaaaac aagtgaaaag tagtgacggc + 361 aaaacaaaag aaaaaatcaa gtttccaaaa atttattata agtctgcaaa aaatagtatt + 421 tttgtttcat ttgaaacagc gggtaataaa tttcaggaaa aatttgaaac cattggtggt + 481 tttttggaaa caacgtttca tgcagataat ttaaataaga ttgatgaaaa aggttttgtt + 541 acctatgaat tagcaacaga tgtgtacaat aagcgtattt ggattaagga tatgcaagca + 601 gacgaaggca aagttcagtt gatgaaaggg ttgtattggc actttgataa agatccacat + 661 ttgttattag gtggaggcac tggtggaggt aaaactttta caattttgtc tctgatctat + 721 gcgctttgtc gagtgggtga ggtggaaatc tgtgacccga aaaattctga cttgatggct + 781 ttaggcaagt taccgctatt tgcaggaaaa gttcatactg gaaaaaaaga tattactcaa + 841 tgcttggaaa atacagtgga gttaatggaa acacgtttta aaacgatgaa taatagttca + 901 cgttacaaga tggggaaaaa ctatgcgtat tatggattaa aaccaaaatt tgtttttata + 961 gatgaatttg cggcctttaa agctgaattg gcaaatgatt attcaacaga cggagaagta + 1021 gatgagtatc ttacgcaact tattttgaaa gctcgtcaag caggtatttt ctttattgtt + 1081 gcaatgcaac gtccagatgg agaattttta aaaacagcat tacgtgacca atttatgttt + 1141 cgcatgtctg ttggacgtct atctgaaaca ggtattttga tgattttcgg tgatgaaaat + 1201 aaaaataaaa agtttaaata tgttgaaaaa attgacggtc agaaagtcta tggtcgtgga + 1261 tatgtggctc aaggaggagg aactgcacgt gaattttata gtcctcaagt accacaagac + 1321 tttgacttca ttgaagaatt tatcaaaatt tctaaagagt taggttatga agatgttcca + 1381 aaagaagtac aagaagaagt atctcaaaaa atatctaaac atattgataa agaagcactg + 1441 gcagaaattg atgaagaatt tcaagcagaa aaaaatcagt tatcagagtt aagtgagaaa + 1501 tattctgctt aaaaagaagt cacataaatt agttggaaaa ggaaggtttt tatggattct + 1561 aatgaaataa ttaaacgtgt cagagaacga gtatatagag aagtaaagaa aaaatatact + 1621 agagacgatt tggatactcg tattcaagat gtattatatt atcgttcaga aacatatatg + 1681 aaattagtta gctttgcaaa tggtaaaaga attaagaagt tagctgatcc tagaaaattt + 1741 gaaaagttta tggatacaaa aggagtaaag attgttgcag aagtacttga tggattgaat + 1801 aatcaaccaa aaatgcaagc tatggaatac gaacaaaagg tacttactaa agtacgtcaa + 1861 tggtatcaga aaaagaatca ccctgagctt gtagatttgg aagaagaagc atttgaacaa + 1921 cttgtagaaa aaaatatcat ctataaaaaa atgaagaaaa ggttgtatga agaacaggat + 1981 aatcaaggtt ttgtttattc ggataatttt gatatgcaat tgattcgtga ttcatgtgat + 2041 attgaagagg ctctatactt agatatcacg ttgggggatt actaagtgaa aggagtaaaa + 2101 agaaagtgaa atttaaatct cgagtacaaa tgttaaacta tgtgattggg aagaaaccag + 2161 acatcatgaa aacagttgat cggagagcag aaaaagaggg ggttacagat gataatagat + 2221 tacttgatta taaattatta gtaatttctg ttttagatgc agaagctgtt tacgatgaag + 2281 atagaccaat tgaggaaaag aaattttcaa catcacaaga agttgataag tatttagaaa + 2341 caagatttaa ggagcatatg gattctgctt ggaaagaaat gtttaataag taggtgtgga + 2401 aaatgtattc atttgatcaa gtaattttag agctagacaa tgctattgct ttgaaaacac + 2461 tgaaaaattg ggcaaatagg attgaaaaaa tgactgatac acgttttgaa cgccgatatg + 2521 caaagaatag tgcaggtcat agttatagct ataaggtttt ttctattaaa gatatagaaa + 2581 attttcaaga gttgctacgt ttgcgtgaaa ataatgttcc gcttaatgag gcaataaatg + 2641 aagtatttat gtctgatgag aataaagcta cacatattaa taaacaagaa attgaggatt + 2701 tgaaaagaga tatgaggcaa cttttaaagg tgtctcaatc tattcttgcg gagaatgcaa + 2761 gcttaaaaga gagaattggt gcattagaac gaggaacagc tgaataacgg ctgtttattt + 2821 gaaggattgt tgctagtaat aagtatacgt gggcgaagca gagcgaaagc ccacgtatac + 2881 ttttagcaac ggaaaatcct tttaaaatga acagacgtaa ggtgggcgaa tgcccacctt + 2941 acgtcaaaaa ccccctattt ataacatggg ggtagaattt aatataccgt ataaaaaagt + 3001 tgcttaaaag ctatgtccta caagggattg agcgattatg attttgtcac atttttcgac + 3061 taaagttgtc acattttttg gaagtaggtt taaagcagtg agaggtgttg atttaaagcg + 3121 atatagaaaa gaactaaaat taaaacagca agaattggca agtaaattag gaatcgagcg + 3181 ttcgttaatt tcaaaaatag aatctggaaa acgtgttatt tctaaagagt tagaacaaaa + 3241 gattattaat gtgttgaact tagatggtgg gcacgcttcg gtagaagcaa aaattgactt + 3301 tttaagaata cgatttaaaa cattagatgt tcggacagtt attgaaaagt tgttgcacat + 3361 ggatatgaac tggtttactc atgaatctag agggttttat cattacacgg aaacattttc + 3421 atatagctct attcggattt ttcggaatcc ggaaaatgtc aatatgggaa ttatgttgga + 3481 cttgtctggc gaaggctgtc gtcaattaga agaaatcttt gaggaagata acaaccgatc + 3541 gtggacagaa ttttttcgct ctctttatga tgacgatatt ttcggtcaag gcattttagt + 3601 agatacaaaa atcactcgaa ttgatattgc tcttgatgaa ttgattgtga aggggcaaga + 3661 aaattttgat ttgtatgttt taaaagaaaa aatggagcag gggcttgttg acaccacatt + 3721 taaaaatttt gattttagcg gtggatttgt ttacgaaaat aaaaaaatgg tgaataaagg + 3781 tttatccctt tattttggta gtcgtcaatc gccactgtat tttaactttt accaaaaaga + 3841 ttacgagtta gcaagaaaag aaagtatgag tgttgaagaa gcaagagaaa aacatgaaat + 3901 taaaaatcga tacgaaattc gcctttctga tgaaaaggcg tttttgtttg tagaatattt + 3961 tctatcaagc ggtgagtcgc tagattggtt agtgaaagaa attattaatc aatctttgac + 4021 tgtctatgac attgaagatg atatgaaagt ttattgtaaa tcttggcatg atgttgttga + 4081 taaattggaa ggcttaaaat tgtctgtgca aggtgaaaaa ccgtctattg aaaagacgct + 4141 tcgatggcta tcaaattatt tagccccttc attaaagatg attaaagaga ttgataatct + 4201 gttaggaaca aatgaattga tggaacgcat tgatttagca gaattaaaag aaaagcatga + 4261 agagattatt gaaatggtga gtgttgatgc aaaagacttg ttgtttacca atagagataa + 4321 taaaagtgtt cggtcctata tggaacgaga gttcgaatta gaagaaatgt acccgtttta + 4381 gaaaggtgga ttgatagaat ggataaagaa ttaatcaaaa aaaatataaa acttatggat + 4441 gccactattg agaaaatgat tcatgtgatg agttctattc ataaacttca attgagtgta + 4501 aaagaacttg aagacagtaa cgaattaact gtttcttatg taaaaagaga atgtacaaaa + 4561 ttggaagaga gggttaagga acttagaccg agtatttcaa gatgtgtttt tatgtatcga + 4621 gaatatgtag aagaatttga aacaaaactt agagatagaa tagttacaga aaagtttttt + 4681 cgaataaaaa ggttctatgg taaagaagta ttagctttta aggaatttca agaaaagtat + 4741 caaaattata ttcctaaaga tattattgat ataaagaaac aagttcgtca gaagctagaa + 4801 gaagttggtt atgaaattga tggtgctttt gagggggatt ttacaagttg ggtaggtgtt + 4861 tatgcgcgtc caaaagataa accgacctat ttagaccctg cgaatgcgga agaagtagtt + 4921 ttacaagaaa aatatagcgt caatggattt aaacaagatt tttcagaatg gtttgaattt + 4981 gaaattaaag ataatgttgt ttatggcatt taataattgt catgtaaaag acatttttta + 5041 ttttctctaa aaggttacta ttttcataat aaatattatt ttgttattgt taatttgagg + 5101 tgaaaggggt atgactaaaa aattaataaa aatatggcgt aaagaaatgt tggcattaat + 5161 gaatgaagac gcagactggt ataggaatga agataccgag cgttttaaac gaatacaaga + 5221 acttgcaaga aagatagaaa cagcaaacac cagacaattt tcttcgcata ttagcaagga + 5281 aagatttgaa gcctatcaaa aaatgggctt acagtttaaa gaaatagcgg aagagttcca + 5341 tataacaact acagcgttac aacaatggcg taaagacaat gggtatccca tttacaacaa + 5401 aaataatcgg aagtgaggaa aataaaatga aaaagatcgc aagtgcaggg ttaagtattt + 5461 tagtcgcaac gggggtagca ggtattgggg gaaatgaagt acaggcagca gaacaagcgc + 5521 aaccaaaaac acctgaaaac agttctacag aacaaccagc agtgaaagct acagaaacaa + 5581 cggagcaagc cattactgaa aaacagcaac aagtaacaga gaaacaagca attgtcgatc + 5641 aaaaacaaca agttgctgac actgcgaaaa aagaaaaaga caccattgat caatctgtta + 5701 aagaccaaca agcagtggtc gatcaaaaca aaggtgcatt ggatcaaagt caacaagcag + 5761 tgactgacca acaagcggtc gtagacgaag caaaaaaagt tgtggatgaa gcaacacctt + 5821 cagccattga aaaagccaaa aaccaagtgg ctacggatac acaagctgtt gatgaccaac + 5881 aaaaagtagt ggatcaagct caagcagacg ttaaccaaca acaagcagtt gtcgaagaaa + 5941 aagcaaaaga aacgaatgct gctaaagtgc aaaatgataa agatcaacaa gcagtaacag + 6001 ctgcgaaaca agaacaagcc aagcttgaag aattagcgaa aaatgcggaa gtggaaaaag + 6061 caaaggctga aaaagaacaa gcagcaaaag aagctgaact ggctaacaaa cagaaagaag + 6121 aagccaaagc taaagatcaa aaaacgaagg acgatcaagc agtcgcagac caacaaactg + 6181 ttgtgacaac tagtcaagag aaagtagcag acgcaaaagc agatacagct gcgaaacagg + 6241 cagacttaac agcgaaagag aatgctttga aagacaagca aacagcaaca aagcaagtgc + 6301 agaatacttt agataaatca aaagaagagc taaaaggaca taaaggaatt aacttgcctg + 6361 ctaacttcac acctgattat tacaaaaaat tatctgaaca agaaaagcaa gcaatggaaa + 6421 aagaagcatt agcattaaat aaagtttttc ctgaaaatca agcagatgtg gcaaaagcaa + 6481 cggaaatgat caatgtcaaa aatcctaccg gaaaacaaaa gcaacaaatg agcgattacg + 6541 ttgtaggact tatcaatgat gttcgcgaaa agcttgggtt acaaaagttg aagatttcta + 6601 accaagctat gagatttgct tgggatgtag caaaatatga taatcccaaa gagtttgatc + 6661 atgacgtaaa tgcaattaat cgtgcagcaa aagaaaatgg ttttaaagag tatccagggc + 6721 aaaactttta tgaaaatcta agtatgggct attttgaaac gattaatggc actatttctc + 6781 aactagagtt tgaaaaagct gctcgaaaaa caattgctga tatgctcttt gacgatgaaa + 6841 gttcagcgta ttctcatata gattcattgc taaaagggga cacaacaaac atggcagttt + 6901 ctatttcagg agatttaaat gatatttcgg caaaaatcca tatcattagc tataatcaat + 6961 caaaattagt tgaagcaaat acctatgaag aaggcactgc tccagtcttt aagagtaaag + 7021 aaacccttca aaaagaagta gcaaccaatc aagaaaaatt agctactgca caacaagcag + 7081 aatcagacgc tcaacaagca aaaagtgcaa gtcagcaagt cttaaataca gccaaaacaa + 7141 cacaagcaac agcagaaaaa gaactatctg ttcataaagc gacattggct agtcttcaag + 7201 cagttgcgac taaaagtacg acaaattatg aagaaaaagt acgacaaact gcaacggcag + 7261 aaaaaagcct tcaacaaaca aaagatcaat tggcaacaat caatgagtta attcagaatc + 7321 gagctgctgt tttagaaaaa gcgaaaacaa acgtttcgga agcacaagca attgaacaaa + 7381 cgtctgctaa agtgctgaaa gaaaaacaag cagcccaaaa agcagaagaa aacacattga + 7441 atagcttgaa ggaagtattg aatttagcaa aagaaaattt aaatcaaaaa caagttgcat + 7501 ttaaaacaag tacacgttca ttgtctcgtt tagaaaatgc tcaaccaaca tacgaaaaag + 7561 cattaaacga gttaaacaaa gcagaagcag cagcggtcca agcacaagaa gcctatgaaa + 7621 attctctgaa atcattggag gaacttaaag aacaacaagc cgttgctaca cttgcttata + 7681 cacaagcaca agaagacctt tctaacgcga agttagagct acagcagtac cagggcgtat + 7741 taagagaatt agaagcacaa caagccgaac agcagcgaca agaagcgttg caagaacaag + 7801 tagcaaaaga acaacaacgc cttgaacgag aagcaaaaca aagccaaacg ttagtagcaa + 7861 gtgctacttc agcagacaaa acacctggtc tccaacagtt atctttttct aaacaaaaag + 7921 aacagccaaa agcacaagca ttaacacatt cagaatctcg taagacgaaa caagtagcaa + 7981 aagccccaga ttctttacca catacaggag aaaaaaataa taaatggtta gctatagctg + 8041 gtctgatatt tgctttgtta ggagctgcgg gtattataag ttttattagt agaaacgaga + 8101 agaaagtaaa aaatatcttt aaaattaaat aataaagtca aaggagcgac atatttcgct + 8161 cctttttatt gtaatcattg gaataaattg aaaaataaaa aaatagccaa ccaaaatggt + 8221 tggctgattg tattaaaggc agaagaatct accaaattta ggagccaacc agtttccgct + 8281 ggtcaacaaa aaataaatgg attgtctcgg atggagacac ttaaatagta cctgacaata + 8341 aaattttagg caacaaaaaa gtcagccgtt ttccgccgac tgactaaacg aataagattt + 8401 gtaaagaagt gatttgtact ttttacacaa ttatgatagc acgttaaatg tttttaacgc + 8461 aaaaaaaagc taagggaatc gaaaaacttt cggttctctt tttgtgagga ggaatggaaa + 8521 taatgatgaa aaagcaacga gctccttgtg ttgcgtgaac tatttttaca aacaaaaaat + 8581 aaaaaatcga ggagaatgat acatgaatca acagactgaa gtaaaaaaac gttttaaaat + 8641 gtataaggca aagaaacact ggattgtagc ccctattctt tttttaggtg tattaggagc + 8701 tgtaggatta gctacagata atgtacaagc cgcggaatta gatacgcaac caggaacaac + 8761 gacggtgcaa cccgataatc ccaacccgca gtcacgaaat gaaacactta aaacggcagt + 8821 atctgaagaa gcagcattac aaaaagacac tacttctcaa ccaaccacag cagaagaagt + 8881 agtgccgaaa ggaattgctg ctgaacaaag ttcagctacc tcaaatgata ccacaaacgt + 8941 ccaacaacca acagcagaag cagaaaaatc agcacaagaa caaccagtag tcagccctga + 9001 aacaaccatt gaacctctag ggcagccaac agaagttgca ccagcagaaa atgacgctaa + 9061 taaatcaacg tcaattccta aagaatttga aacaccagac gttgacaaag cagttgatga + 9121 agcgaaaaaa gatccaaaca ttaccgtcgt ggaaaaacca actgaagact taggaaatgt + 9181 ttcttctaaa gatttagctg caaaagaaaa agaagtagac caactacaaa aagaacaagc + 9241 ccaaaagatt gcccaacaag cagctgaatt aaaagccaaa aatgaaaaaa ttgccaaaga + 9301 aaatgcagaa attgcggcaa aaaataaagc ggaaaaagag cgctacgaaa aagaagtggc + 9361 ggaatacaac aaacacaaaa atgacaaagg ctatgtgaat gaagcaatca gcaaagactt + 9421 agtttttgat tcaagcattg tgactaagga tactaaaata gacaagatta caggtggcaa + 9481 gtttattaaa gcttccgatt ttaacaaagt aaaccaagga caatccaaag atatttttac + 9541 aaaattaagt aaggatatga atggaaaagc aacaggcaac ttccaaagct caaaagtggc + 9601 tgctgttgaa tttggcccta aaggaggata cgcggttctt ttagaaaaaa acaaaccggt + 9661 caatgtcacg tatacaggat taaatgctag ttatttagac cgaaaaatca caaaagcaga + 9721 gttcatttat gaacttcaat ctgcaccaag tcaaagcggc accttaaatg cagtattttc + 9781 gaatgatcct attattacag catttgttgg tactaaaaat gccaatggga aggacgtaaa + 9841 agttcgctta accattaaat tatatgatgc taatggcaaa gaagttttac cagataaaga + 9901 ccatgccttt gcctatgcgt tatcgtcatt aaactctagt ctaggaacaa attatagtgt + 9961 agaacatgcg gaatttgttt cagactttgg ctcaaaaaat gagttcaagt atattaatgg + 10021 ttcctacgta aagaaacaag cggatgggaa attctactca accgaagacc ttgattatgg + 10081 gacaggtcct tcaggactaa aaaatagtga ttgggacgct gtaggtcata aaaatgcgta + 10141 ctatggttct ggtgtaggtc tagctagaga gggtggacgt atttctttct cttttggtat + 10201 gacaaccaaa ggaaaagtca acctatctgg tgcgcaatgg tttgccttta gtaccaattt + 10261 aaatgcgaaa tcaattagac cataccaaaa gaaagggaat ccaaaagaac cagaaaaagc + 10321 aacaattgaa ttcaatcgat acaaagccaa tgtcgttcct gttcttgtgc cgaataaaga + 10381 agtcactgat ggtcagaaaa atatcaatga tttaaatgtg aaacgaggcg attctttaca + 10441 atacattgtg acaggggata cgacagaact tgccaaagta gatccgaaaa cagtgacaaa + 10501 acaagggatt cgggatacct ttgatgcaga aaaagtgacg attgatttat ccaaagtgaa + 10561 ggtttatcaa gcagatgcaa gtctaaacga gaaagactta aaagctgttg ctgcagcgat + 10621 taattcagga aaagctatag acgtgactgc ttcttatgtt cttaatttag atcaaaacac + 10681 cgtcacagca atgatgaaaa ccaacgcaga cggttccgtt gttttagcaa tggggtataa + 10741 atatttactt gtcttgccgt ttgtagtgaa aaatgtagaa ggcgattttg aaaatacagc + 10801 tgttcagctg acaaatgatg gcgaaacggt aacaaataca gtgattaacc atgtgccggt + 10861 tagtaatcct tccaaagatg taaaagcaga taaaaacggt acagttggca gtgtttctct + 10921 acatgataaa gatattccgt tacaaacaaa aatttattat gaagtgaaat cttccgaacg + 10981 tccagctaac tatggcggaa ttaccgaaga atggggcatg aatgatgtct tggacacgac + 11041 ccatgatcgt ttcacaggta aatggcacgc tattacaaac tatgacctta aagtagggga + 11101 caaaacgtta aaagcaggaa cagatatttc tgcctacatt cttttagaaa acaaagacaa + 11161 taaagacttg acgtttacaa tgaatcaagc attattagca gcgttaaatg aaggaagcaa + 11221 taaagtaggc aaacaagctt ggtctgtata tctggaagtc gaacggatca aaacaggtga + 11281 cgtagaaaat acgcaaacag aaaactacaa caaagaactt gttcgttcta atacggtggt + 11341 gacgcataca cctgatgatc caaaaccaac caaagccgtt cacaacaaga aaggggaaga + 11401 tattaaccat ggaaaagttg ctcgtggtga tgttctttct tatgaaatga cgtgggactt + 11461 aaaagggtac gataaagact ttgcttttga cacagtcgat cttgcgacag gagtttcttt + 11521 cttcgatgat tacgacgaaa cgaaagtgac accaatcaaa gacttacttc gtgtcaaaga + 11581 ttctaaaggg gcagacatta cgaaccagtt cacgatctct tgggacgatg ccaaaggcac + 11641 ggtgacaatc tctgccaaag acccacaagc ctttattcta gcgtatggtg ggcaagaatt + 11701 gcgtgtaacg ctccctacaa aagtcaaagc caatgtttct ggtgatgttt ataattcagc + 11761 ggaacaaaat acatttggtc aacgaattaa aacaaatacc gttgtcaacc atattccaaa + 11821 agtgaaccct aaaaaagacg tggttattaa agttggtgac aaacaaagtc aaaatggcgc + 11881 cacaatcaaa ttaggggaga aattcttcta tgaatttaca agtagtgaca ttcctgcaga + 11941 atacgctggt gttgtggaag aatggtcgat tagcgataaa ctagacgtaa aacatgacaa + 12001 atttagtggc caatggtctg tgtttgccaa ttctaatttt gttttagcag acggaaccaa + 12061 agtgaataaa ggggacgaca tttcgaaact attcacgatg acctttgaac aaggggtagt + 12121 taaaatcacg gccagtcaag cgtttttgga tgcgatgaat ctaaaagaaa acaaacacgt + 12181 tgcgcactca tggaaagcgt tcattggtgt agaacgaatt gcggcaggag acgtttacaa + 12241 cacaatcgaa gaatctttca acaatgagaa gattaaaact aatacggtag tgacacatac + 12301 gccagaaaaa ccacaaacac caccagaaaa aacagtgatt gtaccaccaa caccaaaaac + 12361 accacaagca ccagtagagc cattagtggt agaaaaagca agtgtggcac ccgaactacc + 12421 tcatacaggt gaaaaagaaa acaccctatt atctgtacta ggtgccggaa tgctagttgg + 12481 tctggcttgg tttggtttga aaaaacgtga agtgaaataa tgagtaaaaa gaaaaagcac + 12541 tgtgaaaaat cagtgctttt tgtttatttt agaaaaagtg tggtgttgtc aagtgtggat + 12601 aataattttt gcgttaagtt gtatcgtagt atctataata attggagcat ttgtcagtgc + 12661 aacggtcgat gaacgattgg gagacaatat ttttttgcta cttctagtta ttttattttt + 12721 ttggtttatt ttgattttta attgttaagt atattttttt atatgctatt ttaatatatt + 12781 taataagact aggtgaaagt attattaatt cagagatagc tgctattgta gctccgaaat + 12841 aaatattggt catacttttt gaaataaacg acattattat agaaggtagc attataaata + 12901 ttgttggcaa aaaggcaaaa taataagttt ctttggtgat agattcgtta aattctttga + 12961 ttttttgaaa gaaagatagt tgtttatgat agaatatctt aattttctgt atctcgttta + 13021 aagaataaat taagcgcaca aattctttat ttaggtaata agttagaaga acggtatctt + 13081 tgttaatcat tgaaactaag aaaacttttg tattacatgg gatatcattt aaagaaagtt + 13141 cttgggacag tacgtaaaag ttactatgct tacttcttga attagaaggt attaaggttc + 13201 tataaatatc agggagcttt gctattgaga aaataaatac aataaaataa atgataaagt + 13261 aagttaagta agctttaccc tctttagtaa ggttatttat atctgaaaca aaatcggtga + 13321 atgctgtgtc tgtagttatt aatgcataag caaaaatacc agatgaaagt agtaaagagg + 13381 atattagatt aataaaacct ttaaagattt tatataatcc gatttcagtg ggagaggaaa + 13441 aaaggttttt tttaaatgaa tcacttatta cgtatccata gatggaggaa taaattgtga + 13501 aaaagcctat aactataggt aaaagtatac ccactggttt tattaattgg ttaaaaaaat + 13561 caatgttcat ataaataatt actccttata aactttttat aataattata tcaaaaggac + 13621 gtgaatatat gttaataaaa ttaggtacag aattgacgaa agaagaatat gttacaaggt + 13681 atatgcgaaa ctttcagaaa ttattgttgc tcggcgatcg gccaaaagta ttaacgaatc + 13741 gtgaagaaca actcttgcag tatgaaaaag aattatgtgt gctgttttat gaacaattta + 13801 taaagaaaca tcatagagcg cctgatgaag ccacgctaga cgatcaagtt aaagcgaatt + 13861 tcatagagcg ctctaaaatt ttcgctcgat ctcccttagt aatggacgaa ggaaatttta + 13921 cgcaagcaca tattggtcaa ctaaaacggt tgcgtgaatt gcgcatggaa gactatttgc + 13981 cagacaatta tacgcatatt ttacaacgag aagaagaatt ggcacgtaac tattttcgaa + 14041 aacatgatga ttatccattt gggtatgagt gtttatgtat tagtcggtca cgagaagtgg + 14101 tgaaccaagg gctagaaaag ctcttagagg ggttttatga cagttatcag gtttattatc + 14161 gtcggtatcg taaaaatggt tagaaatgga agtaggaata aaaagcgcag gaggtgtagc + 14221 gagcaatggc agaagaatcc aaatatccgc cagtgaaaga aaaatctgtg ttttatcagc + 14281 gttatagtcg tgtttcgacg atttctatgt tgatggattt aggttttagt gtgagtgaag + 14341 cccagattga taatgaagat attgtttata tggaacagta taaagagttt ttattaaata + 14401 aaggaattgt ggaagaaaaa gttcctttta aacgagaatt tgtagcggag tatccattaa + 14461 gcaaaatgcg acgacttttt gtagaaggct attatttacg aaaaccgagt ggtgaaaaaa + 14521 tgtatggtag ctactattac gatttttatt ttaaagagta ttcaggaatg gggatttatt + 14581 gtacgaaagc aacaaaatct ctttttttac atgtttttga acaaagaatt agaaaaataa + 14641 aaaaattaaa acatagattg gaagaatgaa aatgaaaaaa atgattatta ttgccttatt + 14701 cagtacaagc cttttagcag ggggaagcag tgtttctgct tatgcgcaag aatcagaagg + 14761 aaatcttggt gaaacaacag ggagtgtttt accagatgaa ccgaatgtac caactgaccc + 14821 aataacgcca agtgagccag agcaaccaac agagccaagt acaccagagc aaccatcgga + 14881 accgtcaaca ccaaccgaac ctagtgagcc ttcaaaaccg acggatcctt cgttaccaga + 14941 cgaaccgagc gtaccaacag agccaacaac gccaagtaag ccagagcaac caacagagcc + 15001 aacaacgcca agtgtaccag agcaaccaac agagccaagt gtaccagaaa aaccagtaga + 15061 accaaataaa ccaaccgagc cagaaaagcc tgtgccagtt gttcctgaaa aaccagttgt + 15121 accacaacaa ccagagcaac caacagatgt ggtggtaaag ccaaatggag aaattgcaac + 15181 aggagaatct acacaacagc caactgttcc aattgaaacg aataaccttt cagaagtaac + 15241 acatgtccca actgtgacga caccgattga aacagcaagc ggagaagcaa ttgtcgcagt + 15301 ggataagggc gttcctttaa cacaaacggc tgatggatta aaaccgatta aaagtgaata + 15361 taaagtatta ccaagtggca atgtacaagt gaaaagtgct gacggaaaaa tgaaagtact + 15421 tccttacact ggtgaaaaaa tgggcataat tgggtcaatc gctggtgtat gtttgactgt + 15481 tttatcagga atcttaattt ataaaaaacg taaagtgtag tgagtgatgt atgaaatttt + 15541 taaaaaaaat tcaaacaaag gaaaaaagca aaaagagccc aagagttcgt ttactatcac + 15601 aaaaaaaagc gaatcgatta gtattaattg tggggtgtgc tctttttgga ttgtcattga + 15661 ttggtgctat tcgagcaaat gtgatggcag gcaacgtgat ccgcctcagt caacaattag + 15721 aaacaataaa taaagatgtc aaaaaaataa aagaaaaaaa gagagtgtat gattcgtcgg + 15781 cactctcttt ttatgtcaga aattttgtga atgagtatat caattttaat ggaaaagcaa + 15841 cagatgaaga gaaaaaagca cgtgatgaga aattagcgag ttatttttca ggagatttag + 15901 cgttagataa aggaacagac aatgctgcaa aagacttaat tcgtaaatta ttacaagtct + 15961 ctgttactcg tgtagaagaa tctgaagagc tattacttgt gcatgcgagt atatcttata + 16021 aagtgcaaca aaaagggcag gatttactag atacacaaga aattgtttta cctattcaag + 16081 aaaaaaatgg gttgttttct attgttggtc gtccctattt tttagcaatg aatgttccga + 16141 aaggaaaaca ggaaccattg caatcagtga aagaaccgct tgatgttgaa aaaaaagaac + 16201 gacaagggat tgaaaaattt ttacaattat tttttgaaaa atatgcgaag gggaatacag + 16261 aagaattgtc tgttttaatg aaagaaccag ttaaaacaag tggtcaagca actgtgtcgt + 16321 ctattgaaga aaaagaacta cgtttttttg aaacgaaaca aaaaggtgtt acaggcgttc + 16381 aggtgtcagt gaactttaaa gacaaagcaa taaatttcct ttacacggaa gatttttcgt + 16441 tgtggttaac gaaaacagaa aattcgtatt ttgtacaaga gttaaaacat tattatacag + 16501 aaaaggcagg agataaatag tggatacaag taaattaagt caatttatga gtagtggatt + 16561 gattacactt attgcgtttg gtgtgatcat tttaatttta aagcattgga aagaagctgc + 16621 gtggttaaag attggttcag ttattgtcat tgcattgatt ttaaatgact ttgcgacaaa + 16681 taatgggcag aacattttca aagttgtgaa gtgggtctta ggattatttg ggattcagct + 16741 gtagaggaga gaaataatga aacattttga ttattcgagg ggcttaaaag ccccttactc + 16801 attacaagtt attaagtccc caaaagggaa aattgtttgg tattttgcac aacccttaag + 16861 tttagcttat ttagtgatgt tatttttagg gattgtacta acaggcattt tttggaagtt + 16921 tgtaccattg ccgttaattt ttggtattaa tcttaaccta atgattatgt tgtattttcc + 16981 aaataaagtg gctcgatggt atacagaaac agagtttgaa gggaaaacag gattagcttt + 17041 tttaaaagat ggttttgttt atgtaaaaaa ttatgtgtta gacaatagat caattatatc + 17101 ctttgaacga gtaaaggaaa tagaagaatt ttcatttaaa cgttagtaat aagaaagagt + 17161 gtattatggt gaaaacatga aaaaaagaag tgtgttgtaa taacttcttt tttcatgttt + 17221 aaattatgaa tttctatatt ttaaataaag cataactgca cagactatta ttccaataac + 17281 taaaagtagc acagtaacaa tactttcatt tttatctatt tttctttcag gtctatagct + 17341 aggattaatt tgtttggcgt taaaatcagt aattccaaag tctaaagtag gaagtaaaaa + 17401 gagtaataaa ataatatata taattcccgc tataaggaaa ataggggcgc tagtgaaaag + 17461 tgaaataatg gtacctatga ttataggaat taaaatgtat aaaaaggatt tgataacaga + 17521 ttcttttctc attttaatta ccaaattttg ttattcatgt ttcccatatt tcctaaagga + 17581 acacaataac caccgctcat atttttccca cctgtagacc agccaccttt tttaaaataa + 17641 aaacctccag gacaagagct accaaattca gcttctcttg cactagcttc tgagctaaaa + 17701 ataaaaggtg ttaccatcgc gaaaatagtt aaagctaaac aaactttttt cttcatatac + 17761 gttccttctt tccaattatt atttaaaagc tatattgaat atagctttta aataataata + 17821 actgaatgaa aaaaaactgt ccaataatat atagaaattt ctgaaaaatc atttttaatg + 17881 taaattattt taataaaatt aataagaaag gaaaaaaata tgcaactaga atatccatta + 17941 acgaatcata tgaaaagaaa catggtttta actaaagaaa atcaagtgat tgcgtattat + 18001 cgtattcggt cagaaacagt tggcttaaca gattttgaga aaaaaaggaa aacaaagaaa + 18061 aaagtagctc gaacgttgaa acgtttacaa gagaatgagg gttttgaaat tgttctttta + 18121 cctgtgaatg cggatattcg agggaaaatg ggaggtatgc gccaattagt ggacaaagaa + 18181 aatcatagtg tagcggtgga taagttgatg aaaactgctc agtatcttga aaatgaaatt + 18241 ggcatggtct atgagtatat ttggttaatt ggcgtgcctt tagtaaagaa agagcggtca + 18301 attgatatta aagaaacatt ttctacggcg ttaaataatc tatctgaaaa agtggttaaa + 18361 ggacttggtt tagaagtagg agtagcggag gattgggaag aagcttataa agaccaagaa + 18421 caagaagtat atcagaattt gtcagaattg ttagtggaac gattgacaga agacgagttg + 18481 tattactacc aagcctatca atttttaaat aatattcaac atgagaaaaa agaacttttg + 18541 actagtcaga acttagataa tttactagcg aacaaaatca ctccttttcg aggtgggtta + 18601 gaattgtcta atgagtttgg gaaaagttat attgctcatt taccattggg ggattgtggg + 18661 gtcacgattg atggtaatca cttgctagaa ctggttcaaa aaatgtctta tcctgtttct + 18721 gtgaagttac aggctggttt tgctgaaaca aaaggccaat tagcgttatc aggacgttct + 18781 gctcgtgcga gaacacgaac aaagaatatc atggaagaag cacatttagc aggttcaaaa + 18841 caaaaaagaa aaattgttga ggggcaacat tcacttgatg atttagatca aaaaattgat + 18901 gatgatattc cgattattga ttggaaagca gtattagtga ttagtgggaa ttcaaaaaaa + 18961 caattacgtg agcgaaagaa aaatttgatg aatcgactag atagcttagg aattccgtta + 19021 atccgtgcaa cgtttgataa tgtctatctt tttcaagcaa cgttattagg caactttcaa + 19081 cgtttttcaa ccagtaattg gcaacatacg tcgacgttag aaacattttc agagttgaat + 19141 ttctttactt cgttacacgc aggtacaaag acagggtttt atttgggacg tgtggacgcc + 19201 acgttagaag aaaaagaaag tcggaaacaa attgtttctg gctctaaaaa tattgtctat + 19261 atgaatctat tgcttgcaaa caaacagaat attgaaggaa agaaaacaaa taatccccat + 19321 tggttggtat cgggagatac aggtaacggg aaatcagtat tttctaaatg gttatttttg + 19381 tattcttctc tattagatgt gaaagtgtta tacattgatc caaaaaaaga agttcgccaa + 19441 cagtttatgc gaacgattaa tgatccagaa tatcaaagaa aatacccact tgatgtggca + 19501 tttattaaga cgtttaattt tgtgacactt gatgttcgta aaaaagaaaa ccatggggta + 19561 cttgatccaa ttgtattatt tgatgaaaca gaagcaatcg caacggcaaa agctatgtta + 19621 aataatatta acgaggataa gtggaaaatg ccacataaga cggcgattaa tgaaacagta + 19681 gcagaagtgg tggctgaacg gaaagcaggc aaacaagttg gattttggca tgtaattgaa + 19741 cgtttaatta gtcattcaga gaaagacgtt catgaaatgg gtcgtttttt attatctacg + 19801 attaaaggat cgattttaga gttagccttt tctcatggtg aagtagaagg actttctttt + 19861 gagaagaaag tgaccatttt agaaattgaa gatttagatt tacccacaga tcggcacgat + 19921 gaattagacg agaatcaacg gttatctgtg actttaatgt ttgcattagg cacgttttgt + 19981 tcaaaatttg ggtcacgcaa tcggaaagaa gaaacagtca ctttctttga tgaagcgtgg + 20041 atttttcaat cgtcaccaga aggacaaaag attctaaaat cgatgaaacg gatcggacgt + 20101 tcgtttaata attttatggt attaattacg caatcggtca acgatgtgtt agataatggc + 20161 gatggtacag gctttggaac ggtggtttgt ttcgatgaag tggataatcg agaagggatt + 20221 ttacgttatt taaaattacc agtcaatgag attaatttaa aatgggtatc gaatatgatt + 20281 caaggacaat gtctcatgaa agatatgttt aaccaagtga atcgaatcgt gatagacgtt + 20341 atttttgaag aatggttaga actctttgaa acagttgatg atacagaagc gtccgttatg + 20401 gaaaacgatt atgtggcgag gtggtgagaa agtatgaaat atctcattga tagttataca + 20461 gcctatatgg tgaatggtgg tttattagat aaaggatcag aagtcgtcaa ttggttcttt + 20521 attgaattgc cttttttctt tttgcgtatg ggggcaatga ttttcttaat tatggagaat + 20581 gtcatgaatc agtcagatta ttttgttggt aaacaacaag aagcatacga ttattctttg + 20641 gatattttaa aaggatttgg tggaactggg attgtcaaag ggagtttgct tggattggca + 20701 attatcctaa gtgcctatta tctattgtat agcttttttt ctaatcggcg taatttcatg + 20761 aaaagtttgt tgcattattt tgcggtgttt gccttgttta tttgttggtt tggacaagta + 20821 aaaacaattg atggtaaaac gcaaaatggg gcaatttttt tgattagttc ggttagtgag + 20881 atgacaaaac aagtacaagg caagtttact tctaatgtaa actttggagg ggatacaagc + 20941 caagaagtag acgatggaaa gaaaaaagtg tatcaaagcc caatgtttga cgcaacggtt + 21001 ttacagacgt ttaactatgt caatagtggc tcgattgatg gcaaaatggc aaatgggaag + 21061 aagcttgatt atgataaact attagaaaag cctaacctga atgataaaga gaaaaaggat + 21121 tttagagaaa aaagaaatgc ttatattgaa cagcaggaga aagacaatcc ttatttttcg + 21181 caagatacct taaaaactat ggaaaaaagt tttgcggtat ggacgggtgc aacaaattta + 21241 tttattctag caattcctgt gttatatatt aatttaatgt tgtcgttgat acagctgtta + 21301 gttgtctttt taattcttat atttccagtt gttttgttgg cttccttttt tccacggtgt + 21361 caaatgttac tattcaagtt ctttcaaggt ttaattggtg cgttattcac gccaattatt + 21421 tatggtattt ttttatcggt gctgttttgg attaacaaac tgattgatgg ggcattttta + 21481 ggtgtcgcaa aaaaagtcag tggcagcttg cttgaattga tttcaggtag tacggtttat + 21541 atgatcgttt tatttgtagc agtggtagtg aagattgtag ttttacgaaa agtttggaaa + 21601 aataaatatg ccattcttgc ttacttttca aatgggcaag tgtcacagcc tgtgtttgaa + 21661 caaaaagtgg aacagttgtc tgaacgaacg aaagaagtag caactggtgg agcgcaagtt + 21721 gccctaggtg cttatacagg caatctgggc atggtcgcaa acggtgcagg taatattcta + 21781 ccgaatcaag ataaagccat tgaaatgggc caagaacatt ttattgatga taatggttca + 21841 tttactgggg taaaagcggg tttacagtct atttttaggc aaccagtgac agaagaagaa + 21901 caaggggagc tgaacgagga acagttttca gaagtggaaa cggaatcgat agatcatgag + 21961 ttaacagata ttgaagaaat aggacagaca gagttagaat ctgatagttt agataactct + 22021 attattgaat tagagcctat gacgagtgaa gatttagtag tggatgtcga taatatgagt + 22081 tctgatatag atgtggagct agcatcagta gaagaagttg gggaagagcc accaatgaac + 22141 gcagaggaat attcaggtta tgataatgtg attgtgacca actttgatga attggctatg + 22201 gcaagagaag aacgtgatta ttttgacggt ggcaaagaac aagagctagt cgagacaaat + 22261 ggagttgaat tggttaataa tatttatccg tttgttcaaa cagaagagta tctagctaat + 22321 cttgaacaga cagaagccca gttttttgga acagatgagt ttgagaaaga attagaacag + 22381 atagaggagt gggcataacc acttctcttt ttatgttagg tggtgcaaat gaaaaaaatc + 22441 tttttaggaa tccttggctt gtttagcttt ttgtttttat tgcctttttt gttatttttg + 22501 ggcaccatgt caacagaaat tggaaacaat acacaatttc aagcaacaac gccacaagaa + 22561 aaagtagcgc tagaggtatc caattttgtt accaaaaatg gtggatcgtt acaatttgcc + 22621 tctgcatggc taggaaatat ggaacatgag agcggattaa atcctgctag aatacaaagt + 22681 gatttaacgt ttaattcagc gtgggcgttt aatccttcga ccaatggtta tgcgttaggt + 22741 ttagcaatga tggacggtga gcgccgtgta aacttgttaa attttgccaa agaacaaaag + 22801 aaagattggc aggctgtccc agtacaactc gagtatatgt ggaatcatga cggctcagac + 22861 agtgccttgc tgaaacgtat gtcgaaaagc tctgatgtga atcaattagc tgtagatatt + 22921 ttggtacatt gggaacgtgc aggcactaaa aatgatccca acgaacaaat caaacgaaaa + 22981 acaagtgcga ataattggta taagagactg tctacaggtt ctatgggggc aggttcagcc + 23041 aatattggtg gtggcaaaat tgatgtgtta gaacaaatgt tagggcaaac agtcaatgga + 23101 ggtcagtgtt atggggggac ttcttattat gttgaaaaga tgggctttca atctttaatg + 23161 aatacagggc atatgtttgc cagtgaaatt ggcaatgatt atgcgtggga acaatctggc + 23221 tggcaggtaa tcaaaaatcc aaactattct gatgtcaaag caggtgatgt gattaatttc + 23281 gcaatgggcg gttatgcgac cagcgtttac ggtcatactg gaattgtagc tagtgttgaa + 23341 ggtaatggaa agcttgtttt atatgaacag aacgctgaaa aaggacaaat aattgctaaa + 23401 tattttcgac aatggggagc agaatatcct aacgtgacaa gtattgtaag gaagaaatag + 23461 aggagagatt tttgatgaaa aaagaagaaa tggacgttgt ttcattgcgg attataaagc + 23521 tttatttttt aggcattagg gaattaaatt tcccagatta taataaaagg tttcagcaaa + 23581 aagatatgga attgtttatt caactagctg atatgatgga aaatttacca aatcttgatg + 23641 aacaactaat ttatgaatta gaagagttga aagattattt attctatgtc aaaaccgaaa + 23701 agtattcttt aacagttcat gatatgtttt tggaaatgaa atccgagctt gaaaaaataa + 23761 tttagaagaa atgtagtgtc aaaaaagaga gggtgtagtg tcaaaaatgt ttagaaagaa + 23821 aaatggttta acaaatcggc aagtattagc gatttgtaca aaatttcagt gtaaaaagaa + 23881 tgagtttgtc gcccatcgat taagtgatgg gtatttagta gcagtaaata ataaagaata + 23941 ccgagtaaaa ttttcggaag gctttttttc aaaaattgta tatgcaaaag aagtgcaacg + 24001 gaaaggagct cggaaatcgt gaataagcta cctttactta ttttattgtt aggcggagtg + 24061 ttgcttgtta gtggctgtca aagccataag gaagaaaaca agtctagtaa agtatcgaca + 24121 gaagaaacga cagtgattga aacagtagca agggaacaat cgaaggaatc gtttacgagt + 24181 gaagcaacta aaaaacagac agaaacaacg aaattagaag aaccagatca tgtaaaactt + 24241 ctagaagctt atggaaatgc gtatgcgaac tttacaagta ttaatgatcg caatgaaaag + 24301 ctaaagcccc tcatgactga aaaatgtatc aaaaaaaatg gaattgatgt taaaactgga + 24361 gtagcgttag tttccgtagg aaaggttaca acgatttata aaaatgatca acatgaatat + 24421 gctttacttt tggattgtga acaaaatgga acgcagacac gagtgttact tttggctaag + 24481 gtgaagaaca ataaaatttc tgaaatgacc tataattcag ttaagcaaga gtattagaaa + 24541 ctcttgatat taatagattc catgctagat ttatggtaca attacacata ataaataaag + 24601 gagaaaacca atgaaaaaaa gtattgtaac gatgttattt ttgatgttgg ggatagttgt + 24661 atttactagt tgtgggtcta aaatggataa gacagcagaa gagtttaaga ataagattat + 24721 aagtcatgtc ggtgatcttt atgatgaaaa atatcaagaa aaagaattta gctttttaat + 24781 ttacaaagat aaggatacaa atgaatattt agctaaagct ttggttcctt atgaaggtga + 24841 gcctaatagt gtagaatcta agtattttta taatgtaaat aaagaattag aaactattga + 24901 accatttagt ggaggtagaa cgtttgatta tgctaagtct catggtaact atgaggtaat + 24961 atataaatca ggaaaattta aatagaattt gaacttaaga taaattaaat ttatgtttga + 25021 gagcagtgct tagagcattg ctcttttttg ttttaggagg gaaaacaatg ttaattgtaa + 25081 caaaagaaga cctaatgaat ttaggatatg gaaaatatca agcggaagat ataattagga + 25141 aagcaaaggc agtaatggtg tctaagggat acgcatatta cctaaataaa cgattaggaa + 25201 gagttcctgt tggagctgtc gaaagtgtat taggaataga actagaatct atagcaaaag + 25261 aggttgaaac ggttggttaa gtttaaaaaa gataagagag gaaagtatta ttttactgct + 25321 agtttgggtt ttgatgaagt aactggtaag agggttcaaa aaatgcgttc agggttttcc + 25381 acgattaaag aagcaaggga agcttatgcg gaaataatta ataatttcgg taaagaagca + 25441 tattcttcaa atagcacaat gctgtatgaa gaattttttt atactatttt tttgccatat + 25501 tataagggac gggttaaaga aagaacttat aataatagac ttagtatgat aaaaaatact + 25561 tttgcctatt tttttaaaat gaaattgaaa agtatagctc ctgttcatat acagaagtgg + 25621 cagaatgagc tgttggaaca gtatgaaaac acgtatgtaa gaaatattta tggactgttt + 25681 cagatgtctt tagatcgagc tgttgtgtta ggaatgattt cttcaaatcc agcaaagatc + 25741 gttggtaatg tgaaaaaaag taagaaagaa attgattttt ggacaaaaga agaatttgaa + 25801 aaagttgtta agacttttta tgtagaagat tattaccaaa atttttcttt tatttgtatc + 25861 tggttattat ttatgacagg catgagaata ggtgaggcta ctgctttaac ttggaaagat + 25921 gtgaatttag atagaagata tttaacagta aaaaaatcac tatattataa aaatgcgcaa + 25981 acttatgaat tagtgtctcc aaagactaga gccagtatta ggacgattta tttagatgaa + 26041 gatacagttc actatttaag agattggaaa aaaaggcaag atgatgttgg aggaattgaa + 26101 tttattttat cttataattc agttcctact caaaaacata cagtaagaca tattattaaa + 26161 cgtcatgcaa aattggcaga ggtacatgat attaggattc atgctttaag acattctcat + 26221 gcttctttat taataagtat gggtactaat gctttgttga taaaagaacg attgggtcat + 26281 gaagatgtgc aaacaacttt aggtacttat ggacacttat atccaagtag ctccactgaa + 26341 atagctaatg aattgaaggg aatagttaat gttgagttta ctaatcaaaa tatggcttcg + 26401 gaggttacta atcaatttac taaaggagta aaaaaataa +// +