# HG changeset patch # User iuc # Date 1706276320 0 # Node ID d45c95f382d3a2e8664189a2c593c09dedeb0458 # Parent 85c9849b429e9277209e4a49aca6ee044e5bc598 planemo upload for repository https://forgemia.inra.fr/ices_imes_analysis/icescreen commit bc68b3c32a23a39507f54cb6b4453676174712d6 diff -r 85c9849b429e -r d45c95f382d3 icescreen.xml --- a/icescreen.xml Tue Oct 31 16:31:33 2023 +0000 +++ b/icescreen.xml Fri Jan 26 13:38:40 2024 +0000 @@ -1,12 +1,9 @@ - detects and annotates ICEs (Integrative and Conjugative Elements) and IMEs (Integrative and Mobilizable Elements) in Firmicutes genomes. + detects and annotates ICEs (Integrative and Conjugative Elements) and IMEs (Integrative and Mobilizable Elements) in Bacillota genomes. - 1.2.0 - 1 + 1.3.1 + 0 - - ICEscreen - icescreen zip @@ -23,7 +20,7 @@ --galaxy --gbdir ./tmp_icescreen/source_genbank --outdir ./tmp_icescreen - --mode '${taxonomy.mode}' + --phylum '${taxonomy.phylum}' --jobs "\${GALAXY_SLOTS:-4}" > ./tmp_icescreen/ICEscreen_log.txt 2>&1 && cat ./tmp_icescreen/ICEscreen_results/results/${gbname}/icescreen_detection_ME/${gbname}_detected_ME.summary > '${summary}' @@ -49,10 +46,9 @@ -
- - - +
+ +
@@ -129,7 +125,7 @@ - + @@ -138,22 +134,22 @@ - + - + - + - + @@ -180,7 +176,7 @@ **What it does** - ICEscreen is a bioinformatic pipeline for the detection and annotation of ICEs (Integrative and Conjugative Elements) and IMEs (Integrative and Mobilizable Elements) in Firmicutes genomes. The full documentation is available at https://icescreen.migale.inrae.fr. The forge of the project is accessible at https://forgemia.inra.fr/ices_imes_analysis/icescreen. + ICEscreen is a bioinformatic pipeline for the detection and annotation of ICEs (Integrative and Conjugative Elements) and IMEs (Integrative and Mobilizable Elements) in Bacillota genomes. The full documentation is available at https://icescreen.migale.inrae.fr. The forge of the project is accessible at https://forgemia.inra.fr/ices_imes_analysis/icescreen. **Main features of ICEscreen** @@ -193,8 +189,8 @@ There are 3 main output results files generated by ICEscreen: - - Detected Signature Proteins table (`*_detected_SP_withMEIds.csv`): list of the signature proteins detected by the tool and their possible assignment to an ICE or IME element. It is a comma separated table of 48 columns with a one line header. Each line represents a signature protein detected by ICEscreen. - - Detected ICEs/IMEs table (`*_detected_ME.tsv`): list of the ICEs and IMEs elements detected by the tool, including information about the signature proteins they contain. It is a tab separated table of 21 columns, the header is at line #3. Information in this file is similar to the output file _withICEIMEIds.csv (option -m) but centered around a list of ICE / IME structures instead of a list of SPs. + - Detected Signature Proteins table (`*_detected_SP_withMEIds.csv`): list of the signature proteins detected by the tool and their possible assignment to an ICE or IME element. Each line represents a signature protein detected by ICEscreen. + - Detected ICEs/IMEs table (`*_detected_ME.tsv`): list of the ICEs and IMEs elements detected by the tool, including information about the signature proteins they contain. Information in this file is complementary to the _withICEIMEIds.csv file above with each line representing an ICE / IME structures instead of a signature protein. - Results summary (`*_detected_ME.summary`): this file summarizes the main parameters and statistics regarding the ICE / IME structures and the SPs. Other optional and additional output files generated by ICEscreen: diff -r 85c9849b429e -r d45c95f382d3 test-data/NC_004668_137848_164286_detected_ME.tsv --- a/test-data/NC_004668_137848_164286_detected_ME.tsv Tue Oct 31 16:31:33 2023 +0000 +++ b/test-data/NC_004668_137848_164286_detected_ME.tsv Fri Jan 26 13:38:40 2024 +0000 @@ -1,2 +1,2 @@ -ICE_IME_id Segment_number Genome_accession Category_of_element Category_of_integrase Host_ICE_IME_ids Guest_ICE_IME_ids Colocalized_ICE_IME_ids ICEline_format ICE_consensus_superfamily_SP_conj_module ICE_consensus_family_SP_conj_module IME_relaxase_family_domains_blast HMM_family_SP_conj_module Integrase_upstream Integrase_downstream Relaxase Coupling_protein VirB4 List_SP_ordered_genomic_position Other_potential_SP_conj_module_need_manual_curation_and_review Other_potential_integrase_need_manual_curation_and_review Comments_regarding_structure -ID_1 1 NC_004668.1 Complete ICE (C+R+V+I) one integrase Tyr - - - C3.R1:V7.I Tn916 ICESt3 - C:TcpA, R:MOBT, V:VirB4 - EF_RS00760 EF_RS00650 EF_RS00630 EF_RS00720 EF_RS00630, EF_RS00650, EF_RS00720, EF_RS00760 - - Downstream integrase EF_RS00760 has been associated to the structure ID_1 pre-merging because it is adjacent to the conj module and there is no upstream/downstream ambiguity. +ICE_IME_id Segment_number Genome_accession Category_of_element Category_of_integrase Host_ICE_IME_ids Guest_ICE_IME_ids Colocalized_ICE_IME_ids ICEline_format ICE_consensus_superfamily_SP_conj_module ICE_consensus_family_SP_conj_module IME_relaxase_family_domains_blast HMM_family_SP_conj_module Integrase_upstream Integrase_downstream Relaxase Coupling_protein VirB4 List_SP_ordered_genomic_position Start_of_most_upstream_SP Stop_of_most_downstream_SP Other_potential_SP_conj_module_need_manual_curation_and_review Other_potential_integrase_need_manual_curation_and_review Comments_regarding_structure +ID_1 1 NC_004668.1 Complete ICE (C+R+V+I) one integrase Tyr - - - C3.R1:V7.I Tn916 ICESt3 - C:TcpA, R:MOBT, V:VirB4 - EF_RS00760 EF_RS00650 EF_RS00630 EF_RS00720 EF_RS00630, EF_RS00650, EF_RS00720, EF_RS00760 1 26439 - - Downstream integrase EF_RS00760 has been associated to the structure ID_1 pre-merging because it is adjacent to the conj module and there is no upstream/downstream ambiguity. diff -r 85c9849b429e -r d45c95f382d3 test-data/NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.tsv --- a/test-data/NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.tsv Tue Oct 31 16:31:33 2023 +0000 +++ b/test-data/NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.tsv Fri Jan 26 13:38:40 2024 +0000 @@ -1,3 +1,3 @@ -ICE_IME_id Segment_number Genome_accession Category_of_element Category_of_integrase Host_ICE_IME_ids Guest_ICE_IME_ids Colocalized_ICE_IME_ids ICEline_format ICE_consensus_superfamily_SP_conj_module ICE_consensus_family_SP_conj_module IME_relaxase_family_domains_blast HMM_family_SP_conj_module Integrase_upstream Integrase_downstream Relaxase Coupling_protein VirB4 List_SP_ordered_genomic_position Other_potential_SP_conj_module_need_manual_curation_and_review Other_potential_integrase_need_manual_curation_and_review Comments_regarding_structure -ID_1 1 NC_013798.1 Complete ICE (V+C+R+D) one integrase DDE - - - V8.C7.R2.D Tn5252 TnGBS2 - C:VirD4, R:MOBP, V:VirB4 - GALLO_RS01755 GALLO_RS01740 GALLO_RS01695 GALLO_RS01650 GALLO_RS01650, GALLO_RS01695, GALLO_RS01740, GALLO_RS01755 - - Downstream integrase GALLO_RS01755 has been associated to the structure ID_1 pre-merging because it is adjacent to the conj module and there is no upstream/downstream ambiguity. -ID_2 2 NC_020450.1 Complete ICE (C+R+V+I) one integrase Tyr - - - C1.R1:V5.I Tn916 - - C:TcpA, R:MOBT, V:VirB4 - LILO_RS03350 LILO_RS03260 LILO_RS03250 LILO_RS03320 LILO_RS03250, LILO_RS03260, LILO_RS03320, LILO_RS03350 - - Downstream integrase LILO_RS03350 has been associated to the structure ID_2 pre-merging because it is adjacent to the conj module and there is no upstream/downstream ambiguity. +ICE_IME_id Segment_number Genome_accession Category_of_element Category_of_integrase Host_ICE_IME_ids Guest_ICE_IME_ids Colocalized_ICE_IME_ids ICEline_format ICE_consensus_superfamily_SP_conj_module ICE_consensus_family_SP_conj_module IME_relaxase_family_domains_blast HMM_family_SP_conj_module Integrase_upstream Integrase_downstream Relaxase Coupling_protein VirB4 List_SP_ordered_genomic_position Start_of_most_upstream_SP Stop_of_most_downstream_SP Other_potential_SP_conj_module_need_manual_curation_and_review Other_potential_integrase_need_manual_curation_and_review Comments_regarding_structure +ID_1 1 NC_013798.1 Complete ICE (V+C+R+D) one integrase DDE - - - V8.C7.R2.D Tn5252 TnGBS2 - C:VirD4, R:MOBP, V:VirB4 - GALLO_RS01755 GALLO_RS01740 GALLO_RS01695 GALLO_RS01650 GALLO_RS01650, GALLO_RS01695, GALLO_RS01740, GALLO_RS01755 2 24026 - - Downstream integrase GALLO_RS01755 has been associated to the structure ID_1 pre-merging because it is adjacent to the conj module and there is no upstream/downstream ambiguity. +ID_2 2 NC_020450.1 Complete ICE (C+R+V+I) one integrase Tyr - - - C1.R1:V5.I Tn916 - - C:TcpA, R:MOBT, V:VirB4 - LILO_RS03350 LILO_RS03260 LILO_RS03250 LILO_RS03320 LILO_RS03250, LILO_RS03260, LILO_RS03320, LILO_RS03350 2 18868 - - Downstream integrase LILO_RS03350 has been associated to the structure ID_2 pre-merging because it is adjacent to the conj module and there is no upstream/downstream ambiguity.