Mercurial > repos > iuc > idba_ud
diff macros.xml @ 1:2ed5c0795f99 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/idba_ud commit 61e1699e65d6fd9f4f73650ed8463b37cd701344
author | iuc |
---|---|
date | Mon, 05 Aug 2019 15:59:42 -0400 |
parents | fdaf2375d405 |
children | 1542a2fffe0c |
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--- a/macros.xml Fri Sep 21 15:25:56 2018 -0400 +++ b/macros.xml Mon Aug 05 15:59:42 2019 -0400 @@ -1,8 +1,128 @@ <macros> + <token name="@IDBA_VERSION@">1.1.3</token> <xml name="requirements"> <requirements> - <requirement type="package" version="1.1.3">idba</requirement> + <requirement type="package" version="@IDBA_VERSION@">idba</requirement> <yield/> </requirements> </xml> + + <xml name="main_input"> + <param argument="--read" type="data" format="fasta" label="Fasta read file. Lower or equal to 600b"/> + <param argument="--long_read" type="data" format="fasta" optional="true" label="Fasta long read file. More than 600b"/> + </xml> + + <token name="@MAIN_INPUT@"> + --read '$read' + #if $long_read: + --long_read '$long_read' + #end if + </token> + + <xml name="levels_input"> + <param argument="--read_level_2" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for second level scaffolds"/> + <param argument="--read_level_3" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for third level scaffolds"/> + <param argument="--read_level_4" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for fourth level scaffolds"/> + <param argument="--read_level_5" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for fifth level scaffolds"/> + </xml> + <token name="@LEVELS_INPUT@"> + #if $read_level_2: + --read_level_2 '$read_level_2' + #end if + #if $read_level_3: + --read_level_3 '$read_level_3' + #end if + #if $read_level_4: + --read_level_4 '$read_level_4' + #end if + #if $read_level_5: + --read_level_5 '$read_level_5' + #end if + </token> + + <xml name="kmer_options" token_maxk_default="" token_step_default=""> + <param argument="--mink" type="integer" value="20" max="312" label="Minimum k value. Lower or equal to 312"/> + <param argument="--maxk" type="integer" value="@MAXK_DEFAULT@" max="312" label="Maximum k value. Lower or equal to 312"/> + <param argument="--step" type="integer" value="@STEP_DEFAULT@" label="Increment of k-mer of each iteration"/> + <param argument="--inner_mink" type="integer" value="10" label="Inner minimum k value"/> + <param argument="--inner_step" type="integer" value="5" label="Inner increment of k-mer"/> + <param argument="--prefix" type="integer" value="3" label="Prefix length used to build sub k-mer table"/> + <param argument="--min_count" type="integer" value="2" label="Minimum multiplicity for filtering k-mer when building the graph"/> + <param argument="--min_support" type="integer" value="1" label="Minimum supoort in each iteration"/> + <param argument="--seed_kmer" type="integer" value="30" label="Seed kmer size for alignment"/> + </xml> + <token name="@KMER_OPTIONS@"> + --mink $mink + --maxk $maxk + --step $step + --inner_mink $inner_mink + --inner_step $inner_step + --prefix $prefix + --min_count $min_count + --min_support $min_support + --seed_kmer $seed_kmer + </token> + + <xml name="filter_options"> + <param argument="--min_contig" type="integer" value="200" label="Minimum size of contig"/> + <param argument="--similar" type="float" value="0.95" label="Similarity for alignment"/> + <param argument="--max_mismatch" type="integer" value="3" label="Max mismatch of error correction"/> + </xml> + <token name="@FILTER_OPTIONS@"> + --min_contig $min_contig + --similar $similar + --max_mismatch $max_mismatch + </token> + + <xml name="min_pairs_filter"> + <param argument="--min_pairs" type="integer" value="3" label="Minimum number of pairs"/> + </xml> + <token name="@MIN_PAIRS_FILTER@"> + --min_pairs $min_pairs + </token> + <xml name="other_options"> + <param name="other" type="select" display="checkboxes" multiple="true" label="Other options"> + <yield/> + <option value="--no_local">Do not use local assembly (--no_local)</option> + <option value="--no_coverage">Do not iterate on coverage (--no_coverage)</option> + <option value="--no_correct">Do not do correction (--no_correct)</option> + <option value="--pre_correction">Perform pre-correction before assembly (--pre_correction)</option> + </param> + </xml> + <token name="@OTHER_OPTIONS@"> + #if $other: + ${" ".join(str($other).split(","))} + #end if + </token> + + <token name="@THREADS@"> + --num_threads \${GALAXY_SLOTS:-1} + </token> + + <xml name="help" token_more_help=""> + <help><![CDATA[ +IDBA is an iterative De Bruijn Graph De Novo Assembler for sequence assembly. Most assemblers based on de Bruijn graph build a de Bruijn graph with a specific k-mer size to perform the assembling task. For all of them, it is very crucial to find a specific value of k. If k is too large, there will be a lot of gap problems in the graph. If k is too small, there will a lot of branch problems. IDBA uses not only one specific k but a range of k values to build the iterative de Bruijn graph. It can keep all the information in graphs with different k values. + +@MORE_HELP@ + +Input: IDBA-* take interleaved paired end data in the FASTA format as input, +i.e. paired-end reads need to be stored in the same FASTA file such that a pair +of reads should be in two consecutive lines. +In Galaxy paired reads in separate FASTQ files can be converted into interleaved +FASTA using the tools: + +* `FASTQ interlacer on paired end read <https://toolshed.g2.bx.psu.edu/view/devteam/fastq_paired_end_interlacer>`_ +* `Samtools extract FASTA or FASTQ from a SAM file <https://toolshed.g2.bx.psu.edu/view/devteam/fastq_to_fasta>`_ + +Note that, IDBA-* assumes that the paired-end reads are in order (->,<-). +If your data is in reverse order (<-,->), please convert it by yourself. + ]]></help> + </xml> + <xml name="citations"> + <citations> + <citation type="doi">10.1007/978-3-642-12683-3_28</citation> + <yield/> + </citations> + </xml> + </macros>