# HG changeset patch
# User iuc
# Date 1581186279 18000
# Node ID 57aa9597cd3101550d4db8bc38f1a7ae73a7b36e

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/idr_download commit 253efabdfea3a1fecc4c0f2c54c0e97a7d7960ab"

diff -r 000000000000 -r 57aa9597cd31 idr_download_by_ids.py
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/idr_download_by_ids.py	Sat Feb 08 13:24:39 2020 -0500
@@ -0,0 +1,271 @@
+import argparse
+import os
+import sys
+
+from matplotlib import pyplot as plt
+from omero.gateway import BlitzGateway  # noqa
+from omero.constants.namespaces import NSBULKANNOTATIONS  # noqa
+
+
+def warn(message, image_identifier):
+    print(
+        'ImageSpecWarning for {0}: {1}'
+        .format(image_identifier, message),
+        file=sys.stderr
+    )
+
+
+def find_channel_index(image, channel_name):
+    channel_name = channel_name.lower()
+    for n, channel in enumerate(image.getChannels()):
+        if channel_name == channel.getLabel().lower():
+            return n
+    # Check map annotation for information (this is necessary for some images)
+    for ann in image.listAnnotations(NSBULKANNOTATIONS):
+        pairs = ann.getValue()
+        for p in pairs:
+            if p[0] == "Channels":
+                channels = p[1].replace(" ", "").split(";")
+                for n, c in enumerate(channels):
+                    for value in c.split(':'):
+                        if channel_name == value.lower():
+                            return n
+    return -1
+
+
+def get_clipping_region(image, x, y, w, h):
+    # If the (x, y) coordinate falls outside the image boundaries, we
+    # cannot just shift it because that would render the meaning of
+    # w and h undefined (should width and height be decreased or the whole
+    # region be shifted to keep them fixed?).
+    # It may be better to abort in this situation.
+    if x < 0 or y < 0:
+        raise ValueError(
+            'Too small upper left coordinate ({0}, {1}) for clipping region.'
+            .format(x, y)
+        )
+    size_x = image.getSizeX()
+    size_y = image.getSizeY()
+    if x >= size_x or y >= size_y:
+        raise ValueError(
+            'Upper left coordinate ({0}, {1}) of clipping region lies '
+            'outside of image.'
+            .format(x, y)
+        )
+    # adjust width and height to the image dimensions
+    if w <= 0 or x + w > size_x:
+        w = size_x - x
+    if h <= 0 or y + h > size_y:
+        h = size_y - y
+    return [x, y, w, h]
+
+
+def confine_plane(image, z):
+    if z < 0:
+        z = 0
+    else:
+        max_z = image.getSizeZ() - 1
+        if z > max_z:
+            z = max_z
+    return z
+
+
+def confine_frame(image, t):
+    if t < 0:
+        t = 0
+    else:
+        max_t = image.getSizeT() - 1
+        if t > max_t:
+            t = max_t
+    return t
+
+
+def download_plane_as_tiff(image, tile, z, c, t, fname):
+    pixels = image.getPrimaryPixels()
+    selection = pixels.getTile(theZ=z, theT=t, theC=c, tile=tile)
+
+    if fname[-5:] != '.tiff':
+        fname += '.tiff'
+    plt.imsave(fname, selection)
+
+
+def download_image_data(
+    image_ids,
+    channel=None, z_stack=0, frame=0,
+    coord=(0, 0), width=0, height=0, region_spec='rectangle',
+    skip_failed=False
+):
+
+    # connect to idr
+    conn = BlitzGateway('public', 'public',
+                        host='idr.openmicroscopy.org',
+                        secure=True)
+    conn.connect()
+
+    try:
+        prefix = 'image-'
+        for image_id in image_ids:
+            if image_id[:len(prefix)] == prefix:
+                image_id = image_id[len(prefix):]
+            image_id = int(image_id)
+            image = conn.getObject("Image", image_id)
+
+            if image is None:
+                image_warning_id = 'Image-ID: {0}'.format(image_id)
+                if skip_failed:
+                    warn(
+                        'Unable to find an image with this ID in the '
+                        'database. Skipping download!',
+                        image_warning_id
+                    )
+                    continue
+                raise ValueError(
+                    '{0}: Unable to find an image with this ID in the '
+                    'database. Aborting!'
+                    .format(image_warning_id)
+                )
+
+            image_name = os.path.splitext(image.getName())[0]
+            image_warning_id = '{0} (ID: {1})'.format(
+                image_name, image_id
+            )
+
+            if region_spec == 'rectangle':
+                tile = get_clipping_region(image, *coord, width, height)
+            elif region_spec == 'center':
+                tile = get_clipping_region(
+                    image,
+                    *_center_to_ul(*coord, width, height)
+                )
+            else:
+                raise ValueError(
+                    'Got unknown value "{0}" as region_spec argument'
+                    .format(region_spec)
+                )
+            if tile[2] < width or tile[3] < height:
+                # The downloaded image region will have smaller dimensions
+                # than the specified width x height.
+                warn(
+                    'Downloaded image dimensions ({0} x {1}) will be smaller '
+                    'than the specified width and height ({2} x {3}).'
+                    .format(tile[2], tile[3], width, height),
+                    image_warning_id
+                )
+
+            ori_z, z_stack = z_stack, confine_plane(image, z_stack)
+            if z_stack != ori_z:
+                warn(
+                    'Specified image plane ({0}) is out of bounds. Using {1} '
+                    'instead.'
+                    .format(ori_z, z_stack),
+                    image_warning_id
+                )
+
+            ori_frame, frame = frame, confine_frame(image, frame)
+            if frame != ori_frame:
+                warn(
+                    'Specified image frame ({0}) is out of bounds. Using '
+                    'frame {1} instead.'
+                    .format(ori_frame, frame),
+                    image_warning_id
+                )
+            # Get the channel index. If the index is not valid, skip the image
+            if channel is None:
+                channel_index = 0
+                num_channels = image.getSizeC()
+                if num_channels > 1:
+                    warn(
+                        'No specific channel selected for multi-channel '
+                        'image. Using first of {0} channels.'
+                        .format(num_channels),
+                        image_warning_id
+                    )
+            else:
+                channel_index = find_channel_index(image, channel)
+                if channel_index == -1:
+                    raise ValueError(
+                        '"{0}" is not a known channel name for image {1}'
+                        .format(channel, image.getName())
+                    )
+
+            # download and save the region as TIFF
+            fname = '_'.join(
+                [image_name, str(image_id)] + [str(x) for x in tile]
+            )
+            download_plane_as_tiff(image, tile, z_stack, channel_index, frame, fname)
+    finally:
+        # Close the connection
+        conn.close()
+
+
+def _center_to_ul(center_x, center_y, width, height):
+    if width > 0:
+        ext_x = (width - 1) // 2
+        ul_x = max([center_x - ext_x, 0])
+        width = center_x + ext_x + 1 - ul_x
+    else:
+        ul_x = 0
+    if height > 0:
+        ext_y = (height - 1) // 2
+        ul_y = max([center_y - ext_y, 0])
+        height = center_y + ext_y + 1 - ul_y
+    else:
+        ul_y = 0
+    return ul_x, ul_y, width, height
+
+
+if __name__ == "__main__":
+    p = argparse.ArgumentParser()
+    p.add_argument(
+        'image_ids', nargs='*', default=[],
+        help='one or more IDR image ids for which to retrieve data (default: '
+             'read ids from stdin).'
+    )
+    p.add_argument(
+        '-c', '--channel',
+        help='name of the channel to retrieve data for '
+             '(note: the first channel of each image will be downloaded if '
+             'left unspecified)'
+    )
+    region = p.add_mutually_exclusive_group()
+    region.add_argument(
+        '--rectangle', nargs=4, type=int, default=argparse.SUPPRESS,
+        help='specify a clipping region for the image as x y width height, '
+             'where x and y give the upper left coordinate of the rectangle '
+             'to clip to. Set width and height to 0 to extend the rectangle '
+             'to the actual size of the image.'
+    )
+    region.add_argument(
+        '--center', nargs=4, type=int, default=argparse.SUPPRESS,
+        help='specify a clipping region for the image as x y width height, '
+             'where x and y define the center of a width x height rectangle. '
+             'Set either width or height to 0 to extend the region to the '
+             'actual size of the image along the x- or y-axis.\n'
+             'Note: Even values for width and height will be rounded down to '
+             'the nearest odd number.'
+    )
+    p.add_argument(
+        '-f', '--frame', type=int, default=0
+    )
+    p.add_argument(
+        '-z', '--z-stack', type=int, default=0
+    )
+    p.add_argument(
+        '--skip-failed', action='store_true'
+    )
+    args = p.parse_args()
+    if not args.image_ids:
+        args.image_ids = sys.stdin.read().split()
+    if 'center' in args:
+        args.coord, args.width, args.height = (
+            args.center[:2], args.center[2], args.center[3]
+        )
+        args.region_spec = 'center'
+        del args.center
+    elif 'rectangle' in args:
+        args.coord, args.width, args.height = (
+            args.rectangle[:2], args.rectangle[2], args.rectangle[3]
+        )
+        args.region_spec = 'rectangle'
+        del args.rectangle
+    download_image_data(**vars(args))
diff -r 000000000000 -r 57aa9597cd31 idr_download_by_ids.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/idr_download_by_ids.xml	Sat Feb 08 13:24:39 2020 -0500
@@ -0,0 +1,257 @@
+<?xml version="1.0"?>
+<tool id="idr_download_by_ids" name="IDR Download" version="0.9" profile="18.09">
+    <description>- download images from the Image Data Resource using image IDs</description>
+    <macros>
+        <xml name="region_spec" token_pos="upper-left corner">
+            <param name="x_coord" type="integer" value="0" min="0"
+            label="x-coordinate of region @POS@" />
+            <param name="y_coord" type="integer" value="0" min="0"
+            label="y-coordinate of region @POS@" />
+            <param name="width" type="integer" value="0" min="0"
+            label="Region width"
+            help="Set to zero to extend the rectangle maximally along the x-axis of the image" />
+            <param name="height" type="integer" value="0" min="0"
+            label="Region height"
+            help="Set to zero to extend the rectangle maximally along the y-axis of the image" />
+        </xml>
+    </macros>
+    <requirements>
+        <requirement type="package" version="5.6.0">python-omero</requirement>
+        <requirement type="package" version="3.1.3">matplotlib</requirement>
+    </requirements>
+    <command detect_errors="exit_code"><![CDATA[
+        mkdir downloads && cd downloads &&
+        #if str($image_ids.source) == 'link':
+            python -c 'print("${image_ids.id_spec}".replace(",", "|").split("?show=")[-1].replace("|", "\n"))'
+            ## https://idr.openmicroscopy.org/webclient/?show=image-3426274|image-3426275|image-3426276|image-3426277
+        #else:
+            cat '${image_ids.id_spec}'
+        #end if
+        | python '$__tool_directory__/idr_download_by_ids.py' 
+        #set $channel = str($channel).strip()
+        #if $channel:
+            -c '$channel'
+        #end if
+        -f $frame
+        -z $z_section
+        #if str($clip_image.select):
+            ${clip_image.select} ${clip_image.x_coord} ${clip_image.y_coord} ${clip_image.width} ${clip_image.height}
+        #end if
+        $skip_failed
+
+        2> >(tee -a $out_log >&2)
+    ]]></command>
+<inputs>
+        <conditional name="image_ids">
+            <param name="source" type="select" label="How would you like to specify the IDs of images to download?">
+                <option value="link">As text (comma-separated list of IDs or a valid IDR link)</option>
+                <option value="dataset">As a dataset (one image ID per line)</option>
+            </param>
+            <when value="link">
+                <param name="id_spec" type="text"
+                label="Image IDs to download"
+                help="You can enter a single image-id, or a comma (or '|')-separated list of IDs. Alternatively, you can paste here a link to an image selection obtained through the IDR webclient.">
+                    <sanitizer>
+                        <valid><add value="|" /></valid>
+                    </sanitizer>
+                </param>
+            </when>
+            <when value="dataset">
+                <param name="id_spec" type="data" format="txt"
+                label="Select a dataset with image IDs (one per line)" />
+            </when>
+        </conditional>
+        <param name="channel" type="text"
+        label="Name of the channel to download"
+        help="For all image IDs only the specified channel will be downloaded. If left empty, the first channel (whatever this is) will be downloaded by default." />
+        <param name="z_section" type="integer" value="0" min="0"
+        label="z-plane of images to download" />
+        <param name="frame" type="integer" value="0" min="0"
+        label="Image frame to download" />
+        <conditional name="clip_image">
+            <param name="select" type="select"
+            label="Limit the download to a selected region of the image?">
+                <option value="">No, download the entire image plane</option>
+                <option value="--rectangle">Specify a region using its upper-left corner</option>
+                <option value="--center">Specify a width x height region around a central point</option>
+            </param>
+            <when value="" />
+            <when value="--rectangle">
+                <expand macro="region_spec" />
+            </when>
+            <when value="--center">
+                <expand macro="region_spec" pos="center" />
+            </when>
+        </conditional>
+        <param name="skip_failed" type="boolean" checked="false" truevalue="--skip-failed" falsevalue=""
+        label="Skip failed retrievals?"
+        help="By default the tool will fail with an error on the first non-retrievable image ID. Here, you can choose to skip non-retrievable image IDs and continue downloading the available ones instead. The error log will contain warnings about failed IDs in this case." />
+    </inputs>
+    <outputs>
+        <data name="out_log" format="txt" label="${tool.name} error log" />
+        <collection name="output_file" type="list">
+            <discover_datasets pattern="__name_and_ext__" directory="downloads" />
+        </collection>
+    </outputs>
+    <tests>
+        <test>
+            <param name="source" value="dataset" />
+            <param name="id_spec" value="ids.txt" />
+            <param name="channel" value="615.0" />
+            <conditional name="clip_image">
+                <param name="select" value="--rectangle" />
+                <param name="x_coord" value="3" />
+                <param name="y_coord" value="3" />
+                <param name="width" value="5" />
+                <param name="height" value="5" />
+            </conditional>
+            <param name="frame" value="2" />
+            <output_collection name="output_file" type="list">
+                <element name="Centrin_PCNT_Cep215_20110506_Fri-1545_0_SIR_PRJ_1884807_3_3_5_5" file="test1.tiff"/>
+            </output_collection>
+        </test>
+        <test>
+            <param name="source" value="link" />
+            <param name="id_spec" value="1884807" />
+            <param name="channel" value="615.0" />
+            <conditional name="clip_image">
+                <param name="select" value="--rectangle" />
+                <param name="x_coord" value="3" />
+                <param name="y_coord" value="3" />
+                <param name="width" value="5" />
+                <param name="height" value="5" />
+            </conditional>
+            <param name="frame" value="2" />
+            <output_collection name="output_file" type="list">
+                <element name="Centrin_PCNT_Cep215_20110506_Fri-1545_0_SIR_PRJ_1884807_3_3_5_5" file="test1.tiff"/>
+            </output_collection>
+        </test>
+        <test>
+            <param name="source" value="link" />
+            <param name="id_spec" value="1884807" />
+            <param name="channel" value="615.0" />
+            <conditional name="clip_image">
+                <param name="select" value="--center" />
+                <param name="x_coord" value="5" />
+                <param name="y_coord" value="5" />
+                <param name="width" value="5" />
+                <param name="height" value="5" />
+            </conditional>
+            <param name="frame" value="2" />
+            <output_collection name="output_file" type="list">
+                <element name="Centrin_PCNT_Cep215_20110506_Fri-1545_0_SIR_PRJ_1884807_3_3_5_5" file="test1.tiff"/>
+            </output_collection>
+        </test>
+        <test>
+            <param name="source" value="link" />
+            <param name="id_spec" value="https://idr.openmicroscopy.org/webclient/?show=image-9036708|image-9036710|image-9036711" />
+            <param name="channel" value="Spo20(51-91)" />
+            <param name="z_section" value="4" />
+            <conditional name="clip_image">
+                <param name="select" value="" />
+            </conditional>
+            <param name="frame" value="20" />
+            <output_collection name="output_file" type="list" count="3">
+                <element name="171101_LeadingEdgeDeletionPSMMovies01_15_R3D_9036711_0_0_1024_1024">
+                    <assert_contents>
+                        <has_size value="4194510" />
+                    </assert_contents>
+                </element>
+            </output_collection>
+            <output name="out_log">
+                <assert_contents>
+                    <has_text text="(ID: 9036710): Specified image plane (4) is out of bounds"/>
+                    <has_n_lines n="1"/>
+                </assert_contents>
+            </output>
+        </test>
+        <test expect_failure="true">
+            <!-- Test behavior with non-existing image-ID 9036708999 -->
+            <param name="source" value="link" />
+            <param name="id_spec" value="https://idr.openmicroscopy.org/webclient/?show=image-9036708999|image-9036710|image-9036711" />
+            <param name="channel" value="Spo20(51-91)" />
+            <param name="z_section" value="4" />
+            <conditional name="clip_image">
+                <param name="select" value="" />
+            </conditional>
+            <param name="frame" value="20" />
+        </test>
+        <test>
+            <!-- Repeat test with non-existing image-ID 9036708999,
+            but use skip-failed option -->
+            <param name="source" value="link" />
+            <param name="id_spec" value="https://idr.openmicroscopy.org/webclient/?show=image-9036708999|image-9036710|image-9036711" />
+            <param name="channel" value="Spo20(51-91)" />
+            <param name="z_section" value="4" />
+            <conditional name="clip_image">
+                <param name="select" value="" />
+            </conditional>
+            <param name="frame" value="20" />
+            <param name="skip_failed" value="true" />
+            <output_collection name="output_file" type="list" count="2">
+                <element name="171101_LeadingEdgeDeletionPSMMovies01_15_R3D_9036711_0_0_1024_1024">
+                    <assert_contents>
+                        <has_size value="4194510" />
+                    </assert_contents>
+                </element>
+            </output_collection>
+            <output name="out_log">
+                <assert_contents>
+                    <has_text text="9036708999: Unable to find an image with this ID in the database. Skipping download!" />
+                    <has_text text="(ID: 9036710): Specified image plane (4) is out of bounds" />
+                    <has_n_lines n="2"/>
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    <help><![CDATA[
+Download image data from the IDR_ (Image Data Resource) - a public repository
+of reference image datasets from published scientific studies.
+
+.. _IDR: https://idr.openmicroscopy.org/about/
+
+-----
+
+.. class:: infomark
+
+**Input**
+
+A set of image IDs as can be obtained from the IDR webclient_ like this:
+
+1. select the images you want to download
+2. click on the 'Link' button on the top right of the page and copy the provided
+   URL
+3. paste the URL into the input field of this tool, for example: ::
+
+    https://idr.openmicroscopy.org/webclient/?show=image-9036708|image-9036710|image-9036711
+
+Alternatively, you can simply provide a list of known image IDs in the text
+input field (comma or '|'-separated ), or as an input file (each ID on a
+separate line).
+
+Most images in the IDR have more than two dimensions. Thus, there are
+parameters available which allow you to select a particular recording channel,
+z-plane or time frame to download.
+You can also select a region which will be used to crop the images, rather
+than downloading the entire file.
+
+.. _webclient: https://idr.openmicroscopy.org/
+
+
+-----
+
+.. class:: infomark
+
+**Output**
+
+Downloaded images will be saved in TIFF format. If you selected multiple image
+IDs to download, these will be saved as elements of a collection.
+
+The accompanying error log dataset may hold valuable information about requests
+that could not be fulfilled exactly as specified, or that were found to be
+ambiguous.
+    ]]></help>
+    <citations>
+        <citation type="doi">10.1038/nmeth.4326</citation>
+    </citations>
+</tool>
diff -r 000000000000 -r 57aa9597cd31 test-data/ids.txt
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/ids.txt	Sat Feb 08 13:24:39 2020 -0500
@@ -0,0 +1,1 @@
+1884807
diff -r 000000000000 -r 57aa9597cd31 test-data/test1.tiff
Binary file test-data/test1.tiff has changed