isoformswitchanalyzer: IsoformSwitchAnalyzeR.R annotate

annotate IsoformSwitchAnalyzeR.R @ 0:f3fefb6d8254 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380

author	iuc
date	Tue, 24 Jan 2023 18:37:14 +0000
parents
children	2c4e879a81cf

rev	line source
0 f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	1 # Load the IsoformSwitchAnalyzeR library
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	2 library(IsoformSwitchAnalyzeR,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	3 quietly = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	4 warn.conflicts = FALSE)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	5 library(argparse, quietly = TRUE, warn.conflicts = FALSE)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	6 library(dplyr, quietly = TRUE, warn.conflicts = FALSE)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	7
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	8 # setup R error handling to go to stderr
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	9 options(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	10 show.error.messages = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	11 error = function() {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	12 cat(geterrmessage(), file = stderr())
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	13 q("no", 1, FALSE)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	14 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	15 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	16
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	17 # we need that to not crash galaxy with an UTF8 error on German LC settings.
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	18 loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	19
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	20 ################################################################################
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	21 ### Input Processing
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	22 ################################################################################
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	23
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	24
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	25 # Collect arguments from command line
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	26 parser <- ArgumentParser(description = "IsoformSwitcheR R script")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	27
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	28 parser$add_argument("--modeSelector")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	29 parser$add_argument("--parentDir", required = FALSE, help = "Parent directory")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	30 parser$add_argument("--readLength",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	31 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	32 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	33 help = "Read length (required for stringtie)")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	34 parser$add_argument("--annotation", required = FALSE, help = "Annotation")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	35 parser$add_argument("--transcriptome", required = FALSE, help = "Transcriptome")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	36 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	37 "--fixStringTieAnnotationProblem",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	38 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	39 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	40 help = "Fix StringTie annotation problem"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	41 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	42 parser$add_argument("--countFiles", required = FALSE, help = "Count files")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	43 parser$add_argument("--toolSource", required = FALSE, help = "Tool source")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	44 parser$add_argument("--rObject", required = FALSE, help = "R object")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	45 parser$add_argument("--IFcutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	46 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	47 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	48 help = "IFcutoff")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	49 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	50 "--geneExpressionCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	51 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	52 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	53 help = "Gene expression cutoff"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	54 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	55 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	56 "--isoformExpressionCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	57 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	58 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	59 help = "Isoform expression cutoff"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	60 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	61 parser$add_argument("--alpha",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	62 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	63 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	64 help = "")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	65 parser$add_argument("--dIFcutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	66 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	67 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	68 help = "dIF cutoff")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	69 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	70 "--onlySigIsoforms",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	71 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	72 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	73 help = "Only significative isoforms"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	74 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	75 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	76 "--filterForConsequences",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	77 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	78 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	79 help = "Filter for consequences"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	80 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	81 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	82 "--removeSingleIsformGenes",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	83 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	84 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	85 help = "Remove single isoform genes"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	86 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	87 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	88 "--keepIsoformInAllConditions",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	89 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	90 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	91 help = "Keep isoform in all conditions"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	92 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	93 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	94 "--correctForConfoundingFactors",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	95 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	96 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	97 help = "Correct for confunding factors"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	98 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	99 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	100 "--overwriteIFvalues",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	101 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	102 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	103 help = "Overwrite IF values"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	104 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	105 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	106 "--reduceToSwitchingGenes",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	107 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	108 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	109 help = "Reduce to switching genes"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	110 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	111 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	112 "--reduceFurtherToGenesWithConsequencePotential",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	113 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	114 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	115 help = "Reduce further to genes with consequence potential"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	116 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	117 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	118 "--keepIsoformInAllConditions2",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	119 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	120 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	121 help = "Keep isoform in ll conditions"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	122 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	123 parser$add_argument("--minORFlength",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	124 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	125 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	126 help = "")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	127 parser$add_argument("--orfMethod", required = FALSE, help = "ORF methods")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	128 parser$add_argument("--PTCDistance",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	129 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	130 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	131 help = "")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	132 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	133 "--removeShortAAseq",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	134 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	135 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	136 help = "Remove short aminoacid sequences"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	137 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	138 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	139 "--removeLongAAseq",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	140 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	141 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	142 help = "Remove long aminoacid sequences"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	143 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	144 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	145 "--removeORFwithStop",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	146 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	147 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	148 help = "Remove ORF with stop codon"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	149 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	150 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	151 "--onlySwitchingGenes",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	152 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	153 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	154 help = "Only switching genes"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	155 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	156 parser$add_argument("--analysisMode", required = FALSE, help = "Analyze all isoforms with differential usage or single genes")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	157 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	158 "--genesToPlot",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	159 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	160 default = 10,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	161 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	162 help = "Number of genes to plot"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	163 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	164 parser$add_argument("--gene", required = FALSE, help = "Gene ID to analyze")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	165 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	166 "--sortByQvals",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	167 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	168 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	169 help = "Sort genes by Q-val values"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	170 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	171 parser$add_argument("--countGenes",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	172 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	173 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	174 help = "Count genes")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	175 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	176 "--asFractionTotal",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	177 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	178 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	179 help = "Plot gene expresson as fraction of total"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	180 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	181 parser$add_argument("--plotGenes",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	182 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	183 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	184 help = "Plot genes instead of isoforms")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	185 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	186 "--simplifyLocation",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	187 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	188 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	189 help = "Simplify localtion"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	190 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	191 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	192 "--removeEmptyConsequences",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	193 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	194 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	195 help = "Remove empty consequences"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	196 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	197 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	198 "--analysisOppositeConsequence",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	199 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	200 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	201 help = "Analysi opposite consequences"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	202 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	203 parser$add_argument("--pathToCPATresultFile",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	204 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	205 help = "Path to CPAT result file")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	206 parser$add_argument("--pathToCPC2resultFile",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	207 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	208 help = "Path to CPC2 result file")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	209 parser$add_argument("--pathToPFAMresultFile",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	210 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	211 help = "Path to PFAM result file")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	212 parser$add_argument("--pathToNetSurfP2resultFile",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	213 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	214 help = "Path to NetSurfP2 result file")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	215 parser$add_argument("--pathToSignalPresultFile",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	216 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	217 help = "Path to signalP result file")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	218 parser$add_argument("--pathToIUPred2AresultFile",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	219 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	220 help = "Path to IUPred2A result file")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	221 parser$add_argument("--codingCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	222 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	223 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	224 help = "Codding cutoff")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	225 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	226 "--removeNoncodingORFs",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	227 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	228 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	229 help = "Remove non-coding ORFs"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	230 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	231 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	232 "--minSignalPeptideProbability",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	233 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	234 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	235 help = "Minimul signal peptide probability"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	236 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	237 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	238 "--smoothingWindowSize",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	239 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	240 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	241 help = "Smoothing windows size"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	242 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	243 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	244 "--probabilityCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	245 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	246 type = "double",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	247 help = "Probability cutoff"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	248 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	249 parser$add_argument("--minIdrSize",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	250 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	251 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	252 help = "Min Idr size")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	253 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	254 "--annotateBindingSites",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	255 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	256 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	257 help = "Annotate binding sites"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	258 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	259 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	260 "--minIdrBindingSize",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	261 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	262 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	263 help = "Minimun Idr binding size"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	264 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	265 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	266 "--minIdrBindingOverlapFrac",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	267 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	268 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	269 help = ""
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	270 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	271 parser$add_argument("--ntCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	272 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	273 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	274 help = "Nucleotide cutoff")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	275 parser$add_argument("--ntFracCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	276 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	277 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	278 help = "Nucleotide fraction cutoff")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	279 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	280 "--ntJCsimCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	281 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	282 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	283 help = "Nucleotide Jaccard simmilarity cutoff"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	284 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	285 parser$add_argument("--AaCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	286 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	287 type = "integer",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	288 help = "Aminoacid cutoff")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	289 parser$add_argument("--AaFracCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	290 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	291 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	292 help = "Aminoacid fraction cutoff")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	293 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	294 "--AaJCsimCutoff",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	295 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	296 type = "numeric",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	297 help = "Aminoacid Jaccard similarity cutoff"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	298 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	299 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	300 "--removeNonConseqSwitches",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	301 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	302 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	303 help = "Remove switches without consequences"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	304 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	305 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	306 "--rescaleTranscripts",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	307 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	308 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	309 help = "Rescale transcripts"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	310 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	311 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	312 "--reverseMinus",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	313 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	314 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	315 help = "Reverse minus"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	316 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	317 parser$add_argument(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	318 "--addErrorbars",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	319 action = "store_true",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	320 required = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	321 help = "Add error bars"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	322 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	323
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	324
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	325 args <- parser$parse_args()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	326
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	327 # Data import
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	328 ###################
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	329
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	330 if (args$modeSelector == "data_import") {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	331
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	332 quantificationData <- importIsoformExpression(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	333 parentDir = args$parentDir,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	334 addIsofomIdAsColumn = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	335 readLength = args$readLength
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	336 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	337
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	338 ### Make design matrix
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	339 myDesign <- data.frame(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	340 sampleID = colnames(quantificationData$abundance)[-1],
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	341 condition = gsub(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	342 "[[:digit:]]+",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	343 "",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	344 colnames(quantificationData$abundance)[-1]
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	345 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	346 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	347
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	348 if (args$toolSource == "stringtie") {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	349 SwitchList <- importRdata(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	350 isoformCountMatrix = quantificationData$counts,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	351 isoformRepExpression = quantificationData$abundance,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	352 designMatrix = myDesign,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	353 isoformExonAnnoation = args$annotation,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	354 isoformNtFasta = args$transcriptome,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	355 showProgress = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	356 fixStringTieAnnotationProblem = args$fixStringTieAnnotationProblem
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	357 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	358 } else {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	359 SwitchList <- importRdata(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	360 isoformCountMatrix = quantificationData$counts,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	361 isoformRepExpression = quantificationData$abundance,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	362 designMatrix = myDesign,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	363 isoformExonAnnoation = args$annotation,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	364 isoformNtFasta = args$transcriptome,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	365 showProgress = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	366 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	367 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	368
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	369
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	370 geneCountMatrix <- extractGeneExpression(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	371 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	372 extractCounts = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	373 addGeneNames = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	374 addIdsAsColumns = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	375 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	376
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	377 if (args$countFiles == "collection") {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	378
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	379 expressionDF <- data.frame(geneCountMatrix)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	380
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	381 myDesign$condition[length(myDesign$condition)]
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	382
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	383 dataframe_factor1 <- expressionDF %>% select(matches(myDesign$condition[1]))
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	384 dataframe_factor2 <- expressionDF %>% select(matches(myDesign$condition[length(myDesign$condition)]))
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	385
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	386
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	387 lf1 <- as.list(as.data.frame(dataframe_factor1))
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	388 sampleNames1 <- colnames(as.data.frame(dataframe_factor1))
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	389
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	390 lf2 <- as.list(as.data.frame(dataframe_factor2))
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	391 sampleNames2 <- colnames(as.data.frame(dataframe_factor2))
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	392
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	393 geneNames <- row.names(as.data.frame(expressionDF))
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	394
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	395
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	396 for (index in seq_along(lf1)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	397 tabular_expression <- data.frame(geneNames, lf1[index])
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	398 colnames(tabular_expression) <-
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	399 c("Geneid", sampleNames1[index])
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	400 filename <-
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	401 paste(sampleNames1[index], "dataset.tabular", sep = "_")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	402 output_path <- paste("./count_files/factor1/", filename, sep = "")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	403 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	404 tabular_expression,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	405 output_path,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	406 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	407 row.names = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	408 quote = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	409 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	410 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	411 for (index in seq_along(lf2)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	412 tabular_expression <- data.frame(geneNames, lf2[index])
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	413 colnames(tabular_expression) <-
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	414 c("Geneid", sampleNames2[index])
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	415 filename <-
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	416 paste(sampleNames2[index], "dataset.tabular", sep = "_")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	417 output_path <- paste("./count_files/factor2/", filename, sep = "")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	418 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	419 tabular_expression,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	420 output_path,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	421 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	422 row.names = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	423 quote = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	424 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	425 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	426 } else if (args$countFiles == "matrix") {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	427 expressionDF <- data.frame(geneCountMatrix)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	428 geneNames <- row.names(expressionDF)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	429
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	430 expressionDF <- cbind(geneNames, expressionDF)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	431 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	432 as.data.frame(expressionDF),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	433 "./count_files/matrix.tabular",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	434 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	435 row.names = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	436 quote = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	437 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	438 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	439 as.data.frame(myDesign),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	440 "./count_files/samples.tabular",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	441 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	442 row.names = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	443 quote = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	444 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	445 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	446
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	447 save(SwitchList, file = "SwitchList.Rda")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	448
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	449 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	450
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	451 if (args$modeSelector == "first_step") {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	452
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	453 # First part of the analysis
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	454 #############################
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	455
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	456 load(file = args$rObject)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	457
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	458 ### Filter
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	459 SwitchList <- preFilter(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	460 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	461 IFcutoff = args$IFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	462 geneExpressionCutoff = args$geneExpressionCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	463 isoformExpressionCutoff = args$isoformExpressionCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	464 removeSingleIsoformGenes = args$removeSingleIsformGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	465 onlySigIsoforms = args$onlySigIsoforms,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	466 keepIsoformInAllConditions = args$keepIsoformInAllConditions,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	467 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	468 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	469 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	470
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	471 ### Test for isoform switches
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	472 SwitchList <- isoformSwitchTestDEXSeq(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	473 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	474 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	475 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	476 correctForConfoundingFactors = args$correctForConfoundingFactors,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	477 overwriteIFvalues = args$overwriteIFvalues,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	478 reduceToSwitchingGenes = args$reduceToSwitchingGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	479 reduceFurtherToGenesWithConsequencePotential = args$reduceFurtherToGenesWithConsequencePotential,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	480 onlySigIsoforms = args$onlySigIsoforms,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	481 keepIsoformInAllConditions = args$keepIsoformInAllConditions2,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	482 showProgress = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	483 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	484
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	485 SwitchList <- analyzeNovelIsoformORF(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	486 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	487 analysisAllIsoformsWithoutORF = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	488 minORFlength = args$minORFlength,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	489 orfMethod = args$orfMethod,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	490 PTCDistance = args$PTCDistance,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	491 startCodons = "ATG",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	492 stopCodons = c("TAA", "TAG", "TGA"),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	493 showProgress = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	494 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	495
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	496 ### Extract Sequences
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	497 SwitchList <- extractSequence(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	498 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	499 onlySwitchingGenes = args$onlySwitchingGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	500 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	501 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	502 extractNTseq = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	503 extractAAseq = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	504 removeShortAAseq = args$removeShortAAseq,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	505 removeLongAAseq = args$removeLongAAseq,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	506 removeORFwithStop = args$removeORFwithStop,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	507 addToSwitchAnalyzeRlist = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	508 writeToFile = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	509 pathToOutput = getwd(),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	510 outputPrefix = "isoformSwitchAnalyzeR_isoform",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	511 forceReExtraction = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	512 quiet = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	513 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	514
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	515 ### Summary
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	516 switchSummary <- extractSwitchSummary(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	517 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	518 filterForConsequences = args$filterForConsequences,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	519 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	520 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	521 onlySigIsoforms = args$onlySigIsoforms,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	522 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	523
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	524 save(SwitchList, file = "SwitchList.Rda")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	525 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	526 switchSummary,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	527 file = "switchSummary.tsv",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	528 quote = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	529 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	530 col.names = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	531 row.names = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	532 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	533
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	534 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	535
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	536 if (args$modeSelector == "second_step") {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	537
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	538 # Second part of the analysis
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	539 #############################
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	540
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	541 load(file = args$rObject)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	542
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	543 ### Add annotation
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	544 if (!is.null(args$pathToCPATresultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	545 SwitchList <- analyzeCPAT(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	546 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	547 pathToCPATresultFile = args$pathToCPATresultFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	548 codingCutoff = args$codingCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	549 removeNoncodinORFs = args$removeNoncodingORFs
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	550 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	551 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	552
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	553 if (!is.null(args$pathToCPC2resultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	554 SwitchList <- analyzeCPC2(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	555 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	556 pathToCPC2resultFile = args$pathToCPC2resultFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	557 removeNoncodinORFs = args$removeNoncodingORFs
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	558 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	559 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	560
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	561 if (!is.null(args$pathToPFAMresultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	562 pfamFiles <- list.files(path = args$pathToPFAMresultFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	563 full.names = TRUE)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	564
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	565 SwitchList <- analyzePFAM(SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	566 pathToPFAMresultFile = pfamFiles,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	567 showProgress = FALSE)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	568 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	569
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	570 if (!is.null(args$pathToNetSurfP2resultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	571 netsurfFiles <- list.files(path = args$pathToNetSurfP2resultFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	572 full.names = TRUE)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	573
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	574 SwitchList <- analyzeNetSurfP2(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	575 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	576 pathToNetSurfP2resultFile = netsurfFiles,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	577 smoothingWindowSize = args$smoothingWindowSize,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	578 probabilityCutoff = args$probabilityCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	579 minIdrSize = args$minIdrSize,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	580 showProgress = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	581 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	582 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	583
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	584 if (!is.null(args$pathToIUPred2AresultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	585 SwitchList <- analyzeIUPred2A(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	586 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	587 pathToIUPred2AresultFile = args$pathToIUPred2AresultFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	588 smoothingWindowSize = args$smoothingWindowSize,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	589 probabilityCutoff = args$probabilityCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	590 minIdrSize = args$minIdrSize,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	591 annotateBindingSites = args$annotateBindingSites,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	592 minIdrBindingSize = args$minIdrBindingSize,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	593 minIdrBindingOverlapFrac = args$minIdrBindingOverlapFrac,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	594 showProgress = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	595 quiet = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	596 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	597 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	598
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	599 if (!is.null(args$pathToSignalPresultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	600 signalpFiles <- list.files(path = args$pathToSignalPresultFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	601 full.names = TRUE)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	602
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	603 SwitchList <- analyzeSignalP(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	604 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	605 pathToSignalPresultFile = signalpFiles,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	606 minSignalPeptideProbability = args$minSignalPeptideProbability
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	607 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	608 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	609
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	610 SwitchList <- analyzeAlternativeSplicing(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	611 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	612 onlySwitchingGenes = args$onlySwitchingGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	613 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	614 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	615 showProgress = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	616 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	617
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	618 SwitchList <- analyzeIntronRetention(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	619 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	620 onlySwitchingGenes = args$onlySwitchingGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	621 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	622 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	623 showProgress = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	624 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	625
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	626 consequences <- c(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	627 "intron_retention",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	628 "NMD_status",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	629 "isoform_seq_similarity",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	630 "ORF_genomic",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	631 "tss",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	632 "tts"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	633 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	634
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	635 if (!is.null(args$pathToCPATresultFile) \|\|
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	636 !is.null(args$pathToCPC2resultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	637 updatedConsequences <- c(consequences, "coding_potential")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	638 consequences <- updatedConsequences
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	639 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	640
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	641 if (!is.null(args$pathToPFAMresultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	642 updatedConsequences <- c(consequences, "domains_identified")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	643 consequences <- updatedConsequences
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	644 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	645
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	646 if (!is.null(args$pathToSignalPresultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	647 updatedConsequences <- c(consequences, "signal_peptide_identified")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	648 consequences <- updatedConsequences
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	649 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	650
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	651 if (!is.null(args$pathToNetSurfP2resultFile) \|\|
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	652 !is.null(args$pathToIUPred2AresultFile)) {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	653 updatedConsequences <- c(consequences, "IDR_identified", "IDR_type")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	654 consequences <- updatedConsequences
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	655 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	656
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	657 SwitchList <- analyzeSwitchConsequences(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	658 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	659 consequencesToAnalyze = consequences,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	660 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	661 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	662 onlySigIsoforms = args$onlySigIsoforms,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	663 ntCutoff = args$ntCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	664 ntJCsimCutoff = args$ntJCsimCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	665 AaCutoff = args$AaCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	666 AaFracCutoff = args$AaFracCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	667 AaJCsimCutoff = args$AaJCsimCutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	668 removeNonConseqSwitches = args$removeNonConseqSwitches,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	669 showProgress = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	670 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	671
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	672
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	673 ### Visual analysis
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	674 # Top genes
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	675
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	676 if (args$analysisMode == "single") {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	677
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	678 outputFile <- file.path(getwd(), "single_gene.pdf")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	679
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	680 pdf(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	681 file = outputFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	682 onefile = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	683 height = 6,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	684 width = 9
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	685 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	686
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	687 switchPlot(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	688 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	689 gene = args$gene,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	690 condition1 = myDesign$condition[1],
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	691 condition2 = myDesign$condition[length(myDesign$condition)],
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	692 IFcutoff = args$IFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	693 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	694 rescaleTranscripts = args$rescaleTranscripts,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	695 reverseMinus = args$reverseMinus,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	696 addErrorbars = args$addErrorbars,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	697 logYaxis = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	698 localTheme = theme_bw(base_size = 8)
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	699 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	700 dev.off()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	701
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	702 } else {
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	703 mostSwitchingGene <-
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	704 extractTopSwitches(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	705 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	706 n = Inf,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	707 filterForConsequences = args$filterForConsequences,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	708 extractGenes = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	709 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	710 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	711 inEachComparison = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	712 sortByQvals = args$sortByQvals
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	713 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	714
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	715 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	716 mostSwitchingGene,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	717 file = "mostSwitchingGene.tsv",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	718 quote = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	719 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	720 col.names = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	721 row.names = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	722 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	723
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	724
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	725 switchPlotTopSwitches(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	726 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	727 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	728 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	729 onlySigIsoforms = args$onlySigIsoforms,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	730 n = args$genesToPlot,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	731 sortByQvals = args$sortByQvals,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	732 pathToOutput = getwd(),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	733 fileType = "pdf"
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	734 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	735
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	736 outputFile <-
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	737 file.path(getwd(), "extractConsequencesSummary.pdf")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	738
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	739 pdf(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	740 file = outputFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	741 onefile = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	742 height = 6,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	743 width = 9
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	744 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	745
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	746 consequenceSummary <- extractConsequenceSummary(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	747 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	748 consequencesToAnalyze = "all",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	749 includeCombined = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	750 asFractionTotal = args$asFractionTotal,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	751 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	752 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	753 plot = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	754 plotGenes = args$plotGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	755 simplifyLocation = args$simplifyLocation,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	756 removeEmptyConsequences = args$removeEmptyConsequences,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	757 returnResult = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	758 localTheme = theme_bw()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	759 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	760 dev.off()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	761
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	762 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	763 consequenceSummary,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	764 file = "consequencesSummary.tsv",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	765 quote = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	766 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	767 col.names = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	768 row.names = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	769 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	770
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	771
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	772 outputFile <- file.path(getwd(), "consequencesEnrichment.pdf")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	773 pdf(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	774 file = outputFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	775 onefile = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	776 height = 6,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	777 width = 9
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	778 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	779 consequenceEnrichment <- extractConsequenceEnrichment(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	780 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	781 consequencesToAnalyze = "all",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	782 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	783 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	784 countGenes = args$countGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	785 analysisOppositeConsequence = args$analysisOppositeConsequence,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	786 plot = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	787 localTheme = theme_bw(base_size = 12),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	788 minEventsForPlotting = 10,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	789 returnResult = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	790 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	791 dev.off()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	792
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	793 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	794 consequenceEnrichment,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	795 file = "consequencesEnrichment.tsv",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	796 quote = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	797 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	798 col.names = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	799 row.names = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	800 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	801
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	802
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	803 outputFile <- file.path(getwd(), "splicingEnrichment.pdf")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	804 pdf(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	805 file = outputFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	806 onefile = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	807 height = 6,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	808 width = 9
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	809 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	810 splicingEnrichment <- extractSplicingEnrichment(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	811 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	812 splicingToAnalyze = "all",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	813 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	814 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	815 onlySigIsoforms = args$onlySigIsoforms,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	816 countGenes = args$countGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	817 plot = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	818 minEventsForPlotting = 10,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	819 returnResult = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	820 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	821 dev.off()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	822
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	823 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	824 splicingEnrichment,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	825 file = "splicingEnrichment.tsv",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	826 quote = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	827 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	828 col.names = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	829 row.names = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	830 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	831
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	832
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	833 outputFile <- file.path(getwd(), "splicingSummary.pdf")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	834 pdf(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	835 file = outputFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	836 onefile = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	837 height = 6,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	838 width = 9
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	839 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	840 splicingSummary <- extractSplicingSummary(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	841 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	842 splicingToAnalyze = "all",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	843 asFractionTotal = args$asFractionTotal,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	844 alpha = args$alpha,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	845 dIFcutoff = args$dIFcutoff,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	846 onlySigIsoforms = args$onlySigIsoforms,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	847 plot = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	848 plotGenes = args$plotGenes,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	849 localTheme = theme_bw(),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	850 returnResult = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	851 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	852 dev.off()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	853
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	854 write.table(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	855 splicingSummary,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	856 file = "splicingSummary.tsv",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	857 quote = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	858 sep = "\t",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	859 col.names = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	860 row.names = FALSE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	861 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	862
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	863
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	864 ### Volcano like plot:
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	865 outputFile <- file.path(getwd(), "volcanoPlot.pdf")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	866 pdf(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	867 file = outputFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	868 onefile = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	869 height = 6,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	870 width = 9
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	871 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	872 ggplot(data = SwitchList$isoformFeatures, aes(x = dIF, y = -log10(isoform_switch_q_value))) +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	873 geom_point(aes(color = abs(dIF) > 0.1 &
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	874 isoform_switch_q_value < 0.05), # default cutoff
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	875 size = 1) +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	876 geom_hline(yintercept = -log10(0.05), linetype = "dashed") + # default cutoff
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	877 geom_vline(xintercept = c(-0.1, 0.1), linetype = "dashed") + # default cutoff
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	878 facet_wrap(~ condition_2) +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	879 scale_color_manual("Signficant\nIsoform Switch", values = c("black", "red")) +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	880 labs(x = "dIF", y = "-Log10 ( Isoform Switch Q Value )") +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	881 theme_bw()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	882 dev.off()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	883
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	884
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	885 ### Switch vs Gene changes:
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	886 outputFile <- file.path(getwd(), "switchGene.pdf")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	887 pdf(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	888 file = outputFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	889 onefile = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	890 height = 6,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	891 width = 9
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	892 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	893 ggplot(data = SwitchList$isoformFeatures,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	894 aes(x = gene_log2_fold_change, y = dIF)) +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	895 geom_point(aes(color = abs(dIF) > 0.1 &
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	896 isoform_switch_q_value < 0.05),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	897 size = 1) +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	898 facet_wrap(~ condition_2) +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	899 geom_hline(yintercept = 0, linetype = "dashed") +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	900 geom_vline(xintercept = 0, linetype = "dashed") +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	901 scale_color_manual("Signficant\nIsoform Switch", values = c("black", "red")) +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	902 labs(x = "Gene log2 fold change", y = "dIF") +
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	903 theme_bw()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	904 dev.off()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	905
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	906 outputFile <- file.path(getwd(), "splicingGenomewide.pdf")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	907 pdf(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	908 file = outputFile,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	909 onefile = FALSE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	910 height = 6,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	911 width = 9
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	912 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	913 splicingGenomeWide <- extractSplicingGenomeWide(
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	914 SwitchList,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	915 featureToExtract = "all",
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	916 splicingToAnalyze = c("A3", "MES", "ATSS"),
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	917 plot = TRUE,
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	918 returnResult = TRUE
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	919 )
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	920 dev.off()
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	921 }
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	922 save(SwitchList, file = "SwitchList.Rda")
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	923
f3fefb6d8254 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/isoformswitchanalyzer commit 2c61e4c6151000201dd9a8323722a380bc235380 iuc parents: diff changeset	924 }

Mercurial > repos > iuc > isoformswitchanalyzer

annotate IsoformSwitchAnalyzeR.R @ 0:f3fefb6d8254 draft