annotate ivar_removereads.xml @ 10:ee29337f905c draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 8ce6fd9aee543d9e62db33a9c95f79d8dc4e6dea
author iuc
date Wed, 13 Jul 2022 15:20:33 +0000
parents 8d36959b000d
children 7f1cfa4c0e32
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
10
ee29337f905c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 8ce6fd9aee543d9e62db33a9c95f79d8dc4e6dea
iuc
parents: 9
diff changeset
1 <tool id="ivar_removereads" name="ivar removereads" version="@VERSION@+galaxy3" profile="21.01">
0
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
2 <description>Remove reads from trimmed BAM file</description>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
3 <macros>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
4 <import>macros.xml</import>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
5 </macros>
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
6 <expand macro="requirements">
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
7 <requirement type="package" version="3.8.1">python</requirement>
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
8 </expand>
0
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
9 <expand macro="version_command" />
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
10 <command detect_errors="exit_code"><![CDATA[
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
11 cp '$input_bed' binding_sites.bed &&
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
12 python '$__tool_directory__/sanitize_bed.py' binding_sites.bed &&
8
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
13 #if $amplicons.computed == "yes"
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
14 python '$__tool_directory__/write_amplicon_info_file.py' binding_sites.bed amplicon_info.tsv &&
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
15 #else
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
16 ln -s '$amplicon_info' amplicon_info.tsv &&
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
17 #end if
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
18 ivar getmasked
8
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
19 -i '$variants_tsv' -b binding_sites.bed -f amplicon_info.tsv -p masked_primers &&
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
20
8
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
21 python '$__tool_directory__/completemask.py' masked_primers.txt amplicon_info.tsv &&
0
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
22 ln -s '$input_bam' sorted.bam &&
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
23 ln -s '${input_bam.metadata.bam_index}' sorted.bam.bai &&
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
24
10
ee29337f905c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 8ce6fd9aee543d9e62db33a9c95f79d8dc4e6dea
iuc
parents: 9
diff changeset
25 ivar removereads
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
26 -i sorted.bam
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
27 -b binding_sites.bed
0
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
28 -p removed_reads.bam
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
29 -t masked_primers.txt
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
30 ]]></command>
0
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
31 <inputs>
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
32 <param name="input_bam" argument="-i" type="data" format="bam"
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
33 label="Bam input"
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
34 help="BAM dataset, preprocessed with ivar trim, to remove reads from" />
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
35 <param name="variants_tsv" type="data" format="tabular"
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
36 label="Variants input"
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
37 help="This dataset will be scanned for variants that affect primer binding sites and needs to be in tabular format with affected chromosome names in the first, and positions in the second column. If there is a header line, the name of the second column should be POS." />
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
38 <param name="input_bed" argument="-b" type="data" format="bed" label="Primer binding sites information"
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
39 help="The same six-column BED dataset that served as input to ivar trim"/>
8
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
40 <conditional name="amplicons">
9
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
41 <param name="computed" type="select" label="Compute amplicon info from BED file" help="For suitable primer binding site datasets amplicon info can be computed directly (see tool help below). For others you will need to provide an extra amplicon info dataset.">
8
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
42 <option value="yes" selected="true">Yes</option>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
43 <option value="no">No</option>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
44 </param>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
45 <when value="yes" />
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
46 <when value="no">
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
47 <param name="amplicon_info" type="data" format="tabular"
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
48 label="Primer to amplicon assignment info"
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
49 help="This input should consist of one line per amplicon with the tab-separated names of all primers used to generate that amplicon."/>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
50 </when>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
51 </conditional>
0
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
52 </inputs>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
53 <outputs>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
54 <data name="output_bam" format="bam" label="${tool.name} on ${on_string}" from_work_dir="removed_reads.bam"/>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
55 </outputs>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
56 <tests>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
57 <test>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
58 <param name="input_bam" value="zika/Z52_a.trimmed.sorted.bam"/>
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
59 <param name="variants_tsv" value="zika/primers_Z52_consensus.tsv"/>
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
60 <param name="input_bed" value="zika/db/zika_primers_consensus.bed"/>
8
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
61 <conditional name="amplicons">
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
62 <param name="computed" value="no" />
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
63 <param name="amplicon_info" value="zika/db/pair_information.tsv"/>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
64 </conditional>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
65 <output name="output_bam" file="zika/Z52_a.masked.bam" compare="sim_size" delta="100000" />
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
66 </test>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
67 <test>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
68 <param name="input_bam" value="zika/Z52_a.trimmed.sorted.bam"/>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
69 <param name="variants_tsv" value="zika/primers_Z52_consensus.tsv"/>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
70 <param name="input_bed" value="zika/db/zika_primers_consensus.bed"/>
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
71 <conditional name="amplicons">
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
72 <param name="computed" value="yes" />
28a6f1908fcc "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 6dae6f97a45a61b1f10be4227d978584624c3b3d"
iuc
parents: 5
diff changeset
73 </conditional>
0
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
74 <output name="output_bam" file="zika/Z52_a.masked.bam" compare="sim_size" delta="100000" />
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
75 </test>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
76 </tests>
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
77 <help><![CDATA[
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
78 This Galaxy tool combines the functionality of ``ivar getmasked`` and
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
79 ``ivar removereads``. No separate ``ivar getmasked`` step is required when
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
80 using this tool.
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
81
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
82 The wrapper takes as input a BAM dataset of aligned and sorted reads, from
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
83 which the primers listed in the primer binding sites BED input have been
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
84 trimmed with ``ivar trim``.
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
85
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
86 From this input it will remove reads that come from amplicons that have been
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
87 generated with one or more primers that may have been affected in their binding
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
88 by variants listed in the variants input file.
9
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
89 To do its job, the needs to know which primers work together to form an
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
90 amplicon. The tool can try to deduce this info from the names of the primers
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
91 found in the primer info dataset. This will require a primer naming scheme
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
92 following the regex pattern::
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
93
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
94 .*_(?P<amplicon_number>\d+).*_(?P<primer_orientation>L(?:EFT)?|R(?:IGHT)?)
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
95
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
96 *i.e.*, the following schemes will work (and get parsed as):
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
97
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
98 - ``nCoV-2019_1_LEFT`` (forward primer of amplicon 1)
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
99 - ``400_2_out_R`` (reverse primer of amplicon 2)
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
100 - ``QIAseq_163-2_LEFT`` (forward primer of amplicon 163)
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
101
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
102 Alternatively, you can specify the amplicon information explicitly through a
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
103 dataset that lists the names of primers that together form any given amplicon.
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
104 In it, primer names (exactly matching those in the primer info dataset) need to
8d36959b000d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit f09d0bee3e957564beccb1bdb3610de02f639ec7"
iuc
parents: 8
diff changeset
105 be TAB-separated with one line per amplicon.
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
106
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
107 .. class:: Warning mark
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
108
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
109 Preprocessing of the BAM input with ivar trim is essential for this tool to
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
110 work because only ``ivar trim`` can add required primer information to the
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
111 BAM auxillary data of every read.
10
ee29337f905c planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 8ce6fd9aee543d9e62db33a9c95f79d8dc4e6dea
iuc
parents: 9
diff changeset
112
4
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
113 ivar documentation can be found at `<https://andersen-lab.github.io/ivar/html/manualpage.html>`__.
ee2beb764a7b "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
iuc
parents: 0
diff changeset
114 ]]></help>
0
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
115 <expand macro="citations" />
bd2a7d1316b9 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 4b0b7fb6c79dcd437392a496a99301d124c9f1dd"
iuc
parents:
diff changeset
116 </tool>