Mercurial > repos > iuc > limma_voom
diff limma_voom.xml @ 8:00a42b66e522 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/limma_voom commit 48125e8638453668f889a67791421f14d3ebe623
author | iuc |
---|---|
date | Tue, 15 May 2018 02:36:36 -0400 |
parents | e6a4ff41af6b |
children | 4255881bebb1 |
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--- a/limma_voom.xml Wed May 09 13:27:14 2018 -0400 +++ b/limma_voom.xml Tue May 15 02:36:36 2018 -0400 @@ -1,4 +1,4 @@ -<tool id="limma_voom" name="limma" version="3.34.9.3"> +<tool id="limma_voom" name="limma" version="3.34.9.4"> <description> Perform differential expression with limma-voom or limma-trend </description> @@ -72,6 +72,10 @@ #end if #end if +#if $out.plots: + -P $out.plots +#end if + #if $out.normCounts: -x #end if @@ -202,9 +206,9 @@ <!-- Contrasts --> <repeat name="rep_contrast" title="Contrast" min="1" default="1"> - <param name="contrast" type="text" label="Contrast of Interest" help="Names of two groups to compare separated by a hyphen e.g. Mut-WT. If the order is Mut-WT the fold changes in the results will be up/down in Mut relative to WT. If you have more than one contrast enter each separately using the Insert Contrast button below. For more info, see Chapter 8 in the limma User's guide: https://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf"> + <param name="contrast" type="text" label="Contrast of Interest" help="Names of two groups to compare separated by a hyphen e.g. Mut-WT. If the order is Mut-WT the fold changes in the results will be up/down in Mut relative to WT. If you have more than one contrast enter each separately using the Insert Contrast button below. For differences between contrasts use e.g. (Mut1-WT)-(Mut2-WT). For more info, see Chapter 8 in the limma User's guide: https://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf"> <validator type="empty_field" /> - <validator type="regex" message="Please only use letters, numbers or underscores">^[\w-]+$</validator> + <validator type="regex" message="Please only use letters, numbers or underscores">^[\(\w\)-]+$</validator> </param> </repeat> @@ -250,6 +254,15 @@ <!-- Output Options --> <section name="out" expanded="false" title="Output Options"> + <param name="plots" type="select" display="checkboxes" multiple="True" optional="True" label="Additional Plots" help="Select additional plots to output in the report and as PDFs"> + <option value="d">Density Plots (if filtering)</option> + <option value="c">CpmsVsCounts Plots (if filtering on cpms)</option> + <option value="b">Box Plots (if normalising)</option> + <option value="x">MDS Extra (Dims 2vs3 and 3vs4)</option> + <option value="m">MD Plots for individual samples</option> + <option value="h">Heatmaps (top DE genes) </option> + <option value="s">Stripcharts (top DE genes)</option> + </param> <param name="normCounts" type="boolean" truevalue="1" falsevalue="0" checked="false" label="Output Normalised Counts Table?" help="Output a file containing the normalised counts, these are in log2 counts per million (logCPM). Default: No"> @@ -278,9 +291,9 @@ <param name="treat" type="boolean" truevalue="1" falsevalue="0" checked="False" label="Test significance relative to a fold-change threshold (TREAT)" help="If you want to apply a cut-off on a fold change the TREAT function can be used, see Help section below. Default: No"/> - <param name="topgenes" type="integer" value="10" min="0" - label="Number of genes to highlight in Volcano plot and Heatmap" - help="The top DE genes will be highlighted in the Volcano plot for each contrast and output in a heatmap PDF. Default: 10."/> + <param name="topgenes" type="integer" value="10" min="0" max="100" + label="Number of genes to highlight in Volcano plot, Heatmap and Stripcharts" + help="The top DE genes will be highlighted in the Volcano plot for each contrast and can be output in heatmap and stripchart PDFs (max 100). Default: 10."/> <param name="normalisationOption" type="select" label="Normalisation Method" help="Default: TMM"> <option value="TMM" selected="true">TMM</option> <option value="RLE">RLE</option>