Mercurial > repos > iuc > macs2
diff macs2_bdgpeakcall.xml @ 5:beb902da6e5f draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/macs2 commit e10f301c7f8c54a7d12df4e631527197baccf70b
author | iuc |
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date | Sat, 08 Apr 2017 08:28:57 -0400 |
parents | bfe57d6e0c4c |
children | acbd3fb47f90 |
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--- a/macs2_bdgpeakcall.xml Mon Feb 06 02:30:37 2017 -0500 +++ b/macs2_bdgpeakcall.xml Sat Apr 08 08:28:57 2017 -0400 @@ -3,58 +3,67 @@ <macros> <import>macs2_macros.xml</import> </macros> - <expand macro="requirements"> - <requirement type="package" version="4.1.0">gnu_awk</requirement> - </expand> + <expand macro="requirements"/> <expand macro="stdio" /> <expand macro="version_command" /> <command> + <![CDATA[ + macs2 bdgpeakcall - --ifile "${ infile }" - --cutoff "${ cutoff }" - --min-length "${ minlen }" - --max-gap "${ maxgap }" - ${ callsummits } + --ifile '${ infile }' + --cutoff '${ cutoff }' + --min-length '${ minlen }' + --max-gap '${ maxgap }' + ${ cutoff_analysis } ${ notrackline } - --ofile "macs2_bdgpeakcall.bdg" - && - awk '!/^track name/' "macs2_bdgpeakcall.bdg" > "${ outfile }" + --ofile '${ outfile }' + + ]]> </command> <inputs> <param name="infile" type="data" format="bedgraph" label="MACS score in bedGraph" /> - <param name="cutoff" type="float" label="Cutoff for peaks" value="5.0" - help="Cutoff depends on which method you used for score track. If the file contains pvalue scores from MACS2, score 5 means pvalue 1e-5. DEFAULT: 5.0 (--cutoff)"/> - <param name="minlen" type="integer" value="200" label="Minimum length of peak" help="better to set it as d value (--min-length)"/> - <param name="maxgap" type="integer" value="30" label="Maximum gap between significant points in a peak" help="better to set it as tag size DEFAULT: 30 (--max-gap)"/> - - <param name="callsummits" type="boolean" truevalue="--call-summits" falsevalue="" checked="False" - label="If set, MACS will use a more sophisticated approach to find all summits in each enriched peak region." - help="(-call-summits)"/> - <param name="notrackline" type="boolean" truevalue="" falsevalue="--no-trackline" checked="False" - label="Include trackline into bedGraph output" - help="required by USCS (--no-trackline)"/> + help="Cutoff depends on which method you used for score track. If the file contains pvalue scores from MACS2, score 5 means pvalue 1e-5. Default=5.0 (--cutoff)"/> + <param name="minlen" type="integer" value="200" label="Minimum length of peak" help="better to set it as d value. Default=200 (--min-length)"/> + <param name="maxgap" type="integer" value="30" label="Maximum gap between significant points in a peak" help="better to set it as tag size Default=30 (--max-gap)"/> + <param name="cutoff_analysis" type="boolean" truevalue="--cutoff-analysis" falsevalue="" checked="False" label="Perform cutoff analysis" help="While set, bdgpeakcall will analyze number or total length of peaks that can be called by different cutoff then output a summary table to help user decide a better cutoff. Note, --min-len and --max-gap may affect the results. Default=False (--cutoff-analysis)"/> + <param name="notrackline" type="boolean" truevalue="" falsevalue="--no-trackline" checked="False" label="Include trackline into bedGraph output" help="required by UCSC Genome Browser (--no-trackline)"/> </inputs> <outputs> - <data name="outfile" format="tabular" label="${tool.name} on ${on_string}" /> + <data name="outfile" format="bedgraph" label="${tool.name} on ${on_string}" /> </outputs> <tests> <test> - <param name="infile" value="callpeak_treatment_part.bdg" ftype="bedgraph" /> + <param name="infile" value="bdgpeakcall_input.bdg" ftype="bedgraph" /> <param name="cutoff" value="5.0"/> <param name="minlen" value="200"/> <param name="maxgap" value="30"/> - <output name="outfile" file="bdgpeakcall_on_callpeak_treatment.tabular"/> + <output name="outfile"> + <assert_contents> + <has_text text="chrV" /> + </assert_contents> + </output> + </test> + <test> + <param name="infile" value="bdgpeakcall_input.bdg" ftype="bedgraph" /> + <param name="cutoff" value="5.0"/> + <param name="minlen" value="200"/> + <param name="maxgap" value="30"/> + <param name="cutoff_analysis" value="true" /> + <output name="outfile" file="bdgpeakcall_output_cutoff.bdg"/> </test> </tests> <help> + <![CDATA[ **What it does** -Call peaks from bedGraph output. -bdgpeakcall from macs2 +This is **bdgpeakcall** utility from the MACS2_ Package. It calls peaks from bedGraph output. + +.. _MACS2: https://github.com/taoliu/MACS @citation@ + ]]> </help> <expand macro="citations" /> </tool>