medaka_consensus_pipeline: annotateVCF.py annotate

author	iuc
date	Tue, 01 Sep 2020 03:08:04 -0400
parents
children	b35de691c42c

rev	line source
3 35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	1 #!/usr/bin/env python3
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	2
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	3 # Takes in VCF file and a samtools mpileup output file
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	4 # Fills in annotation for the VCF file including AF, DP
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	5 # SB, and DP4
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	6 #
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	7 # Usage statement:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	8 # python annotateVCF.py in_vcf.vcf in_mpileup.txt out_vcf.vcf
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	9 #
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	10 # Can generate in_mileup.txt with samtools mpileup (and can restrict which sites to generate pileups for with in_vcf.vcf)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	11
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	12 # 08/24/2020 - Nathan P. Roach, natproach@gmail.com
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	13
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	14 import sys
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	15 from math import isnan, log10
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	16
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	17 from scipy.stats import fisher_exact
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	18
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	19
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	20 def pval_to_phredqual(pval):
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	21 return int(round(-10. * log10(pval)))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	22
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	23
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	24 def parseSimpleSNPpileup(fields, ref_base, alt_base):
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	25 base_to_idx = {
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	26 'A': 0,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	27 'a': 0,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	28 'T': 1,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	29 't': 1,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	30 'C': 2,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	31 'c': 2,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	32 'G': 3,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	33 'g': 3
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	34 }
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	35
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	36 base_to_idx_stranded = {
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	37 'A': 0,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	38 'T': 1,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	39 'C': 2,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	40 'G': 3,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	41 'a': 4,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	42 't': 5,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	43 'c': 6,
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	44 'g': 7
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	45 }
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	46 ref_base2 = fields[2]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	47 counts = [0, 0, 0, 0]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	48 stranded_counts = [0, 0, 0, 0, 0, 0, 0, 0]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	49 ref_idx = base_to_idx[fields[2]]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	50 dp = int(fields[3])
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	51 carrot_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	52 ins_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	53 ins_str = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	54 ins_len = 0
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	55 insertion = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	56 del_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	57 del_str = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	58 del_len = 0
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	59 deletion = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	60 # dollar_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	61 for base in fields[4]:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	62 if carrot_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	63 carrot_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	64 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	65 if ins_len > 0:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	66 insertion += base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	67 ins_len -= 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	68 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	69 if del_len > 0:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	70 deletion += base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	71 del_len -= 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	72 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	73 if ins_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	74 if base.isdigit():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	75 ins_str += base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	76 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	77 ins_len = int(ins_str) - 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	78 insertion = base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	79 ins_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	80 elif del_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	81 if base.isdigit():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	82 del_str += base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	83 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	84 del_len = int(del_str) - 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	85 deletion = base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	86 del_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	87 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	88 if base == '^':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	89 carrot_flag = True
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	90 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	91 elif base == '$':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	92 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	93 elif base == '+':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	94 ins_flag = True
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	95 elif base == '-':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	96 del_flag = True
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	97 elif base == '.':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	98 counts[ref_idx] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	99 stranded_counts[base_to_idx_stranded[ref_base2]] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	100 elif base == ',':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	101 counts[ref_idx] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	102 stranded_counts[base_to_idx_stranded[ref_base2.lower()]] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	103 elif base == 'N' or base == 'n':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	104 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	105 elif base == '*':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	106 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	107 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	108 counts[base_to_idx[base]] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	109 stranded_counts[base_to_idx_stranded[base]] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	110 af = float(counts[base_to_idx[alt_base]]) / float(sum(counts))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	111 if float(sum(stranded_counts[0:4])) == 0:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	112 faf = float("nan")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	113 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	114 faf = float(stranded_counts[base_to_idx_stranded[alt_base]]) / float(sum(stranded_counts[0:4]))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	115 if float(sum(stranded_counts[4:])) == 0:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	116 raf = float("nan")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	117 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	118 raf = float(stranded_counts[base_to_idx_stranded[alt_base.lower()]]) / float(sum(stranded_counts[4:]))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	119 dp4 = [stranded_counts[base_to_idx_stranded[ref_base]],
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	120 stranded_counts[base_to_idx_stranded[ref_base.lower()]],
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	121 stranded_counts[base_to_idx_stranded[alt_base]],
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	122 stranded_counts[base_to_idx_stranded[alt_base.lower()]]]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	123 return (dp, af, faf, raf, dp4)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	124
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	125
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	126 def parseIndelPileup(fields, ref_base, alt_base):
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	127 counts = [0, 0, 0, 0, 0, 0, 0, 0, 0] # indel ref match, indel fwd ref match, indel rev ref match, indel alt match, indel fwd alt match, indel rev alt match, other, other fwd, other rev
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	128 ref_base2 = fields[2]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	129
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	130 carrot_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	131 ins_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	132 ins_str = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	133 ins_len = 0
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	134 del_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	135 del_str = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	136 del_len = 0
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	137 first_iter = True
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	138 forward_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	139 last_seq = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	140 last_seq_code = 'b'
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	141 for base in fields[4]:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	142 if ins_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	143 if base.isdigit():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	144 ins_str += base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	145 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	146 ins_len = int(ins_str)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	147 ins_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	148 if del_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	149 if base.isdigit():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	150 del_str += base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	151 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	152 del_len = int(del_str)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	153 del_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	154 if ins_len > 0:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	155 last_seq += base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	156 last_seq_code = 'i'
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	157 ins_len -= 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	158 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	159 if del_len > 0:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	160 last_seq += base
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	161 last_seq_code = 'd'
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	162 del_len -= 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	163 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	164 if carrot_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	165 carrot_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	166 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	167 if base == '.' or base == ','\
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	168 or base == 'A' or base == 'a'\
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	169 or base == 'C' or base == 'c'\
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	170 or base == 'G' or base == 'g'\
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	171 or base == 'T' or base == 't'\
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	172 or base == 'N' or base == 'n'\
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	173 or base == '>' or base == '<'\
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	174 or base == '*' or base == '#':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	175 if first_iter:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	176 first_iter = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	177 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	178 if last_seq_code == 'i':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	179 if last_seq.upper() == alt_base.upper():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	180 counts[3] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	181 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	182 counts[4] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	183 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	184 counts[5] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	185 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	186 counts[6] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	187 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	188 counts[7] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	189 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	190 counts[8] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	191 elif last_seq_code == 'd':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	192 if last_seq.upper() == ref_base.upper():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	193 counts[3] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	194 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	195 counts[4] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	196 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	197 counts[5] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	198 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	199 counts[6] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	200 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	201 counts[7] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	202 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	203 counts[8] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	204 elif last_seq_code == 'b':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	205 if last_seq.upper() == ref_base.upper():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	206 counts[0] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	207 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	208 counts[1] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	209 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	210 counts[2] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	211 elif last_seq.upper() == alt_base.upper():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	212 counts[3] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	213 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	214 counts[4] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	215 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	216 counts[5] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	217 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	218 counts[6] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	219 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	220 counts[7] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	221 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	222 counts[8] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	223 if base == '.':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	224 last_seq = ref_base2
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	225 forward_flag = True
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	226 last_seq_code = 'b'
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	227 elif base == ',':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	228 last_seq = ref_base2
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	229 forward_flag = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	230 last_seq_code = 'b'
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	231 elif base == '>' or base == '<' or base == '*' or base == '#':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	232 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	233 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	234 forward_flag = base.isupper()
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	235 last_seq = base.upper()
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	236 last_seq_code = 'b'
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	237 elif base == '+':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	238 ins_flag = True
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	239 ins_str = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	240 elif base == '-':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	241 del_flag = True
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	242 del_str = ""
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	243 elif base == '^':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	244 carrot_flag = True
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	245 elif base == '$':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	246 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	247 if first_iter:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	248 first_iter = False
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	249
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	250 if last_seq_code == 'i':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	251 if last_seq.upper() == alt_base.upper():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	252 counts[3] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	253 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	254 counts[4] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	255 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	256 counts[5] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	257 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	258 counts[6] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	259 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	260 counts[7] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	261 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	262 counts[8] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	263 elif last_seq_code == 'd':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	264 if last_seq.upper() == ref_base.upper():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	265 counts[3] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	266 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	267 counts[4] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	268 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	269 counts[5] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	270 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	271 counts[6] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	272 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	273 counts[7] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	274 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	275 counts[8] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	276 elif last_seq_code == 'b':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	277 if last_seq.upper() == ref_base.upper():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	278 counts[0] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	279 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	280 counts[1] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	281 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	282 counts[2] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	283 elif last_seq.upper() == alt_base.upper():
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	284 counts[3] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	285 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	286 counts[4] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	287 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	288 counts[5] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	289 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	290 counts[6] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	291 if forward_flag:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	292 counts[7] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	293 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	294 counts[8] += 1
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	295 dp = int(fields[3])
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	296 af = float(counts[3]) / float(sum([counts[0], counts[3], counts[6]]))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	297 if sum([counts[1], counts[4], counts[7]]) == 0:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	298 faf = float("nan")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	299 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	300 faf = float(counts[4]) / float(sum([counts[1], counts[4], counts[7]]))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	301 if sum([counts[2], counts[5], counts[8]]) == 0:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	302 raf = float("nan")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	303 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	304 raf = float(counts[5]) / float(sum([counts[2], counts[5], counts[8]]))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	305 dp4 = [counts[1], counts[2], counts[4], counts[5]]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	306 return (dp, af, faf, raf, dp4)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	307
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	308
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	309 def annotateVCF(in_vcf_filepath, in_mpileup_filepath, out_vcf_filepath):
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	310 in_vcf = open(in_vcf_filepath, 'r')
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	311 in_mpileup = open(in_mpileup_filepath, 'r')
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	312 out_vcf = open(out_vcf_filepath, 'w')
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	313
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	314 # First pass parsing of input vcf, output headerlines + new headerlines, add VCF sites we care about to to_examine (limits memory usage for sites that don't need annotation)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	315 to_examine = {}
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	316 for line in in_vcf:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	317 if line[0:2] == "##":
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	318 out_vcf.write(line)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	319 elif line[0] == "#":
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	320 out_vcf.write("##annotateVCFVersion=0.1\n")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	321 out_vcf.write("##INFO=<ID=DP,Number=1,Type=Integer,Description=\"Raw Depth\">\n")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	322 out_vcf.write("##INFO=<ID=AF,Number=1,Type=Float,Description=\"Allele Frequency\">\n")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	323 out_vcf.write("##INFO=<ID=FAF,Number=1,Type=Float,Description=\"Forward Allele Frequency\">\n")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	324 out_vcf.write("##INFO=<ID=RAF,Number=1,Type=Float,Description=\"Reverse Allele Frequency\">\n")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	325 out_vcf.write("##INFO=<ID=SB,Number=1,Type=Integer,Description=\"Phred-scaled strand bias at this position\">\n")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	326 out_vcf.write("##INFO=<ID=DP4,Number=4,Type=Integer,Description=\"Counts for ref-forward bases, ref-reverse, alt-forward and alt-reverse bases\">\n")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	327 out_vcf.write(line)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	328 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	329 fields = line.strip().split()
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	330 if fields[0] in to_examine:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	331 to_examine[fields[0]][int(fields[1])] = (fields[3], fields[4])
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	332 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	333 to_examine[fields[0]] = {int(fields[1]): (fields[3], fields[4])}
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	334 in_vcf.close()
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	335 data = {}
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	336
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	337 # Populate data dictionary, which relates chromosome and position to the following:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	338 # depth of coverage
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	339 # allele frequency
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	340 # forward strand allele frequency
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	341 # reverse strand allele frequency
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	342 # dp4 - depth of coverage of ref allele fwd strand, DOC of ref allele rev strand, DOC of alt allele fwd strand, DOC of alt allele rev strand
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	343 for line in in_mpileup:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	344 fields = line.strip().split()
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	345 if fields[0] not in to_examine:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	346 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	347 if int(fields[1]) not in to_examine[fields[0]]:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	348 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	349 (ref_base, alt_base) = to_examine[fields[0]][int(fields[1])]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	350 if len(ref_base.split(',')) > 1: # Can't handle multiple ref alleles
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	351 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	352 if len(alt_base.split(',')) > 1: # Can't handle multiple alt alleles
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	353 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	354 if len(ref_base) > 1 or len(alt_base) > 1:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	355 if len(ref_base) > 1 and len(alt_base) > 1: # Can't handle complex indels
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	356 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	357 data[(fields[0], int(fields[1]))] = parseIndelPileup(fields, ref_base, alt_base)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	358 if len(ref_base) == 1 and len(alt_base) == 1:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	359 data[(fields[0], int(fields[1]))] = parseSimpleSNPpileup(fields, ref_base, alt_base)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	360 in_mpileup.close()
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	361 # Reopen vcf, this time, skip header, annotate all the sites for which there is an entry in data dictionary
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	362 # (Sites without entries have either multiple ref or alt bases, or have complex indels. Not supported (for now), and not reported as a result)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	363 in_vcf = open(in_vcf_filepath, 'r')
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	364 for line in in_vcf:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	365 if line[0] == '#':
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	366 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	367 fields = line.strip().split('\t')
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	368 if (fields[0], int(fields[1])) not in data:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	369 continue
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	370 (dp, af, faf, raf, dp4) = data[(fields[0], int(fields[1]))]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	371 dp2x2 = [[dp4[0], dp4[1]], [dp4[2], dp4[3]]]
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	372 _, p_val = fisher_exact(dp2x2)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	373 sb = pval_to_phredqual(p_val)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	374 if fields[7] == "":
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	375 info = []
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	376 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	377 info = fields[7].split(';')
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	378 info.append("DP=%d" % (dp))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	379 info.append("AF=%.6f" % (af))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	380 if isnan(faf):
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	381 info.append("FAF=NaN")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	382 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	383 info.append("FAF=%.6f" % (faf))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	384 if isnan(raf):
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	385 info.append("RAF=NaN")
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	386 else:
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	387 info.append("RAF=%.6f" % (raf))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	388 info.append("SB=%d" % (sb))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	389 info.append("DP4=%s" % (','.join([str(x) for x in dp4])))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	390 new_info = ';'.join(info)
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	391 fields[7] = new_info
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	392 out_vcf.write("%s\n" % ("\t".join(fields)))
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	393 in_vcf.close()
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	394 out_vcf.close()
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	395
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	396
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	397 if __name__ == "__main__":
35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44" iuc parents: diff changeset	398 annotateVCF(sys.argv[1], sys.argv[2], sys.argv[3])

3

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

1 #!/usr/bin/env python3

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

2

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

3 # Takes in VCF file and a samtools mpileup output file

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

4 # Fills in annotation for the VCF file including AF, DP

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

5 # SB, and DP4

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

6 #

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

7 # Usage statement:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

8 # python annotateVCF.py in_vcf.vcf in_mpileup.txt out_vcf.vcf

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

9 #

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

10 # Can generate in_mileup.txt with samtools mpileup (and can restrict which sites to generate pileups for with in_vcf.vcf)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

11

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

12 # 08/24/2020 - Nathan P. Roach, natproach@gmail.com

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

13

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

14 import sys

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

15 from math import isnan, log10

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

16

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

17 from scipy.stats import fisher_exact

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

18

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

19

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

20 def pval_to_phredqual(pval):

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

21 return int(round(-10. * log10(pval)))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

22

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

23

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

24 def parseSimpleSNPpileup(fields, ref_base, alt_base):

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

25 base_to_idx = {

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

26 'A': 0,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

27 'a': 0,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

28 'T': 1,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

29 't': 1,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

30 'C': 2,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

31 'c': 2,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

32 'G': 3,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

33 'g': 3

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

34 }

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

35

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

36 base_to_idx_stranded = {

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

37 'A': 0,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

38 'T': 1,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

39 'C': 2,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

40 'G': 3,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

41 'a': 4,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

42 't': 5,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

43 'c': 6,

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

44 'g': 7

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

45 }

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

46 ref_base2 = fields[2]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

47 counts = [0, 0, 0, 0]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

48 stranded_counts = [0, 0, 0, 0, 0, 0, 0, 0]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

49 ref_idx = base_to_idx[fields[2]]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

50 dp = int(fields[3])

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

51 carrot_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

52 ins_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

53 ins_str = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

54 ins_len = 0

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

55 insertion = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

56 del_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

57 del_str = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

58 del_len = 0

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

59 deletion = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

60 # dollar_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

61 for base in fields[4]:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

62 if carrot_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

63 carrot_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

64 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

65 if ins_len > 0:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

66 insertion += base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

67 ins_len -= 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

68 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

69 if del_len > 0:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

70 deletion += base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

71 del_len -= 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

72 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

73 if ins_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

74 if base.isdigit():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

75 ins_str += base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

76 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

77 ins_len = int(ins_str) - 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

78 insertion = base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

79 ins_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

80 elif del_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

81 if base.isdigit():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

82 del_str += base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

83 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

84 del_len = int(del_str) - 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

85 deletion = base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

86 del_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

87 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

88 if base == '^':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

89 carrot_flag = True

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

90 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

91 elif base == '$':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

92 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

93 elif base == '+':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

94 ins_flag = True

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

95 elif base == '-':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

96 del_flag = True

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

97 elif base == '.':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

98 counts[ref_idx] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

99 stranded_counts[base_to_idx_stranded[ref_base2]] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

100 elif base == ',':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

101 counts[ref_idx] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

102 stranded_counts[base_to_idx_stranded[ref_base2.lower()]] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

103 elif base == 'N' or base == 'n':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

104 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

105 elif base == '*':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

106 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

107 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

108 counts[base_to_idx[base]] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

109 stranded_counts[base_to_idx_stranded[base]] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

110 af = float(counts[base_to_idx[alt_base]]) / float(sum(counts))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

111 if float(sum(stranded_counts[0:4])) == 0:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

112 faf = float("nan")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

113 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

114 faf = float(stranded_counts[base_to_idx_stranded[alt_base]]) / float(sum(stranded_counts[0:4]))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

115 if float(sum(stranded_counts[4:])) == 0:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

116 raf = float("nan")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

117 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

118 raf = float(stranded_counts[base_to_idx_stranded[alt_base.lower()]]) / float(sum(stranded_counts[4:]))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

119 dp4 = [stranded_counts[base_to_idx_stranded[ref_base]],

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

120 stranded_counts[base_to_idx_stranded[ref_base.lower()]],

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

121 stranded_counts[base_to_idx_stranded[alt_base]],

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

122 stranded_counts[base_to_idx_stranded[alt_base.lower()]]]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

123 return (dp, af, faf, raf, dp4)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

124

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

125

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

126 def parseIndelPileup(fields, ref_base, alt_base):

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

127 counts = [0, 0, 0, 0, 0, 0, 0, 0, 0] # indel ref match, indel fwd ref match, indel rev ref match, indel alt match, indel fwd alt match, indel rev alt match, other, other fwd, other rev

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

128 ref_base2 = fields[2]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

129

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

130 carrot_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

131 ins_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

132 ins_str = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

133 ins_len = 0

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

134 del_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

135 del_str = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

136 del_len = 0

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

137 first_iter = True

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

138 forward_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

139 last_seq = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

140 last_seq_code = 'b'

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

141 for base in fields[4]:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

142 if ins_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

143 if base.isdigit():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

144 ins_str += base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

145 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

146 ins_len = int(ins_str)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

147 ins_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

148 if del_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

149 if base.isdigit():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

150 del_str += base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

151 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

152 del_len = int(del_str)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

153 del_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

154 if ins_len > 0:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

155 last_seq += base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

156 last_seq_code = 'i'

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

157 ins_len -= 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

158 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

159 if del_len > 0:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

160 last_seq += base

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

161 last_seq_code = 'd'

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

162 del_len -= 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

163 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

164 if carrot_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

165 carrot_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

166 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

167 if base == '.' or base == ','\

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

168 or base == 'A' or base == 'a'\

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

169 or base == 'C' or base == 'c'\

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

170 or base == 'G' or base == 'g'\

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

171 or base == 'T' or base == 't'\

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

172 or base == 'N' or base == 'n'\

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

173 or base == '>' or base == '<'\

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

174 or base == '*' or base == '#':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

175 if first_iter:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

176 first_iter = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

177 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

178 if last_seq_code == 'i':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

179 if last_seq.upper() == alt_base.upper():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

180 counts[3] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

181 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

182 counts[4] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

183 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

184 counts[5] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

185 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

186 counts[6] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

187 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

188 counts[7] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

189 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

190 counts[8] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

191 elif last_seq_code == 'd':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

192 if last_seq.upper() == ref_base.upper():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

193 counts[3] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

194 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

195 counts[4] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

196 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

197 counts[5] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

198 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

199 counts[6] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

200 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

201 counts[7] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

202 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

203 counts[8] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

204 elif last_seq_code == 'b':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

205 if last_seq.upper() == ref_base.upper():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

206 counts[0] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

207 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

208 counts[1] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

209 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

210 counts[2] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

211 elif last_seq.upper() == alt_base.upper():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

212 counts[3] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

213 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

214 counts[4] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

215 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

216 counts[5] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

217 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

218 counts[6] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

219 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

220 counts[7] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

221 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

222 counts[8] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

223 if base == '.':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

224 last_seq = ref_base2

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

225 forward_flag = True

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

226 last_seq_code = 'b'

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

227 elif base == ',':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

228 last_seq = ref_base2

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

229 forward_flag = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

230 last_seq_code = 'b'

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

231 elif base == '>' or base == '<' or base == '*' or base == '#':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

232 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

233 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

234 forward_flag = base.isupper()

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

235 last_seq = base.upper()

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

236 last_seq_code = 'b'

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

237 elif base == '+':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

238 ins_flag = True

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

239 ins_str = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

240 elif base == '-':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

241 del_flag = True

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

242 del_str = ""

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

243 elif base == '^':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

244 carrot_flag = True

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

245 elif base == '$':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

246 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

247 if first_iter:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

248 first_iter = False

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

249

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

250 if last_seq_code == 'i':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

251 if last_seq.upper() == alt_base.upper():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

252 counts[3] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

253 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

254 counts[4] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

255 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

256 counts[5] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

257 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

258 counts[6] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

259 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

260 counts[7] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

261 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

262 counts[8] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

263 elif last_seq_code == 'd':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

264 if last_seq.upper() == ref_base.upper():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

265 counts[3] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

266 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

267 counts[4] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

268 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

269 counts[5] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

270 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

271 counts[6] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

272 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

273 counts[7] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

274 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

275 counts[8] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

276 elif last_seq_code == 'b':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

277 if last_seq.upper() == ref_base.upper():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

278 counts[0] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

279 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

280 counts[1] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

281 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

282 counts[2] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

283 elif last_seq.upper() == alt_base.upper():

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

284 counts[3] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

285 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

286 counts[4] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

287 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

288 counts[5] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

289 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

290 counts[6] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

291 if forward_flag:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

292 counts[7] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

293 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

294 counts[8] += 1

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

295 dp = int(fields[3])

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

296 af = float(counts[3]) / float(sum([counts[0], counts[3], counts[6]]))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

297 if sum([counts[1], counts[4], counts[7]]) == 0:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

298 faf = float("nan")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

299 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

300 faf = float(counts[4]) / float(sum([counts[1], counts[4], counts[7]]))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

301 if sum([counts[2], counts[5], counts[8]]) == 0:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

302 raf = float("nan")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

303 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

304 raf = float(counts[5]) / float(sum([counts[2], counts[5], counts[8]]))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

305 dp4 = [counts[1], counts[2], counts[4], counts[5]]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

306 return (dp, af, faf, raf, dp4)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

307

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

308

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

309 def annotateVCF(in_vcf_filepath, in_mpileup_filepath, out_vcf_filepath):

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

310 in_vcf = open(in_vcf_filepath, 'r')

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

311 in_mpileup = open(in_mpileup_filepath, 'r')

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

312 out_vcf = open(out_vcf_filepath, 'w')

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

313

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

314 # First pass parsing of input vcf, output headerlines + new headerlines, add VCF sites we care about to to_examine (limits memory usage for sites that don't need annotation)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

315 to_examine = {}

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

316 for line in in_vcf:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

317 if line[0:2] == "##":

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

318 out_vcf.write(line)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

319 elif line[0] == "#":

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

320 out_vcf.write("##annotateVCFVersion=0.1\n")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

321 out_vcf.write("##INFO=<ID=DP,Number=1,Type=Integer,Description=\"Raw Depth\">\n")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

322 out_vcf.write("##INFO=<ID=AF,Number=1,Type=Float,Description=\"Allele Frequency\">\n")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

323 out_vcf.write("##INFO=<ID=FAF,Number=1,Type=Float,Description=\"Forward Allele Frequency\">\n")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

324 out_vcf.write("##INFO=<ID=RAF,Number=1,Type=Float,Description=\"Reverse Allele Frequency\">\n")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

325 out_vcf.write("##INFO=<ID=SB,Number=1,Type=Integer,Description=\"Phred-scaled strand bias at this position\">\n")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

326 out_vcf.write("##INFO=<ID=DP4,Number=4,Type=Integer,Description=\"Counts for ref-forward bases, ref-reverse, alt-forward and alt-reverse bases\">\n")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

327 out_vcf.write(line)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

328 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

329 fields = line.strip().split()

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

330 if fields[0] in to_examine:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

331 to_examine[fields[0]][int(fields[1])] = (fields[3], fields[4])

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

332 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

333 to_examine[fields[0]] = {int(fields[1]): (fields[3], fields[4])}

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

334 in_vcf.close()

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

335 data = {}

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

336

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

337 # Populate data dictionary, which relates chromosome and position to the following:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

338 # depth of coverage

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

339 # allele frequency

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

340 # forward strand allele frequency

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

341 # reverse strand allele frequency

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

342 # dp4 - depth of coverage of ref allele fwd strand, DOC of ref allele rev strand, DOC of alt allele fwd strand, DOC of alt allele rev strand

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

343 for line in in_mpileup:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

344 fields = line.strip().split()

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

345 if fields[0] not in to_examine:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

346 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

347 if int(fields[1]) not in to_examine[fields[0]]:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

348 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

349 (ref_base, alt_base) = to_examine[fields[0]][int(fields[1])]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

350 if len(ref_base.split(',')) > 1: # Can't handle multiple ref alleles

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

351 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

352 if len(alt_base.split(',')) > 1: # Can't handle multiple alt alleles

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

353 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

354 if len(ref_base) > 1 or len(alt_base) > 1:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

355 if len(ref_base) > 1 and len(alt_base) > 1: # Can't handle complex indels

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

356 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

357 data[(fields[0], int(fields[1]))] = parseIndelPileup(fields, ref_base, alt_base)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

358 if len(ref_base) == 1 and len(alt_base) == 1:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

359 data[(fields[0], int(fields[1]))] = parseSimpleSNPpileup(fields, ref_base, alt_base)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

360 in_mpileup.close()

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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361 # Reopen vcf, this time, skip header, annotate all the sites for which there is an entry in data dictionary

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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362 # (Sites without entries have either multiple ref or alt bases, or have complex indels. Not supported (for now), and not reported as a result)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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363 in_vcf = open(in_vcf_filepath, 'r')

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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364 for line in in_vcf:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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365 if line[0] == '#':

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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366 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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367 fields = line.strip().split('\t')

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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368 if (fields[0], int(fields[1])) not in data:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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369 continue

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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370 (dp, af, faf, raf, dp4) = data[(fields[0], int(fields[1]))]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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371 dp2x2 = [[dp4[0], dp4[1]], [dp4[2], dp4[3]]]

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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372 _, p_val = fisher_exact(dp2x2)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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373 sb = pval_to_phredqual(p_val)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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374 if fields[7] == "":

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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375 info = []

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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376 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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377 info = fields[7].split(';')

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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378 info.append("DP=%d" % (dp))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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379 info.append("AF=%.6f" % (af))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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380 if isnan(faf):

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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381 info.append("FAF=NaN")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

diff changeset

382 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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383 info.append("FAF=%.6f" % (faf))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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384 if isnan(raf):

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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385 info.append("RAF=NaN")

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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386 else:

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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387 info.append("RAF=%.6f" % (raf))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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388 info.append("SB=%d" % (sb))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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389 info.append("DP4=%s" % (','.join([str(x) for x in dp4])))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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390 new_info = ';'.join(info)

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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391 fields[7] = new_info

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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392 out_vcf.write("%s\n" % ("\t".join(fields)))

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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393 in_vcf.close()

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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394 out_vcf.close()

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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395

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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396

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

parents:

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397 if __name__ == "__main__":

35666d44fe7a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit e80b649094384fc6d7a8f917300db3550cc99a44"

iuc

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398 annotateVCF(sys.argv[1], sys.argv[2], sys.argv[3])

Mercurial > repos > iuc > medaka_consensus_pipeline

annotate annotateVCF.py @ 3:35666d44fe7a draft