Mercurial > repos > iuc > medaka_consensus_pipeline
diff medaka_consensus.xml @ 0:ef94bace3a3c draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit 1b97fa7a21a2c85bf4891aed1b7df92685b346c3"
author | iuc |
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date | Sat, 23 May 2020 13:38:26 -0400 |
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children | 551f8c9bc4cf |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/medaka_consensus.xml Sat May 23 13:38:26 2020 -0400 @@ -0,0 +1,168 @@ +<tool id="medaka_consensus_pipeline" name="medaka: Consensus pipeline" version="@TOOL_VERSION@+galaxy0" profile="@PROFILE@"> + <description>Assembly polishing via neural networks</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <expand macro="version_command"/> + <command detect_errors="exit_code"><![CDATA[ + medaka_consensus + ## optional + -m '${m}' + -b $b + -o 'results' + #if 'draft' in str($out).split(',') or 'variants' in str($out).split(',') or 'polished' in str($out).split(',') + -v + #end if + -t \${GALAXY_SLOTS:-4} + ## required + -i '$i' + -d '$d' + + |& tee 'log.txt' + #if 'variants' in str($out).split(',') + && bgzip -d 'results/variants.vcf.gz' + #end if + ]]></command> + <inputs> + <param argument="-i" type="data" format="fastq,fastq.gz,fastqsanger,fastqsanger.gz,fasta,fasta.gz" label="Select basecalls"/> + <param argument="-d" type="data" format="fasta,fasta.gz" label="Select assembly" help="The input assembly should be preprocessed with racon."/> + <expand macro="model"/> + <expand macro="b"/> + <param name="out" type="select" multiple="true" optional="false" label="Select output file(s)" help="'Draft To Consensus', 'Variants' and 'Polished regions in draft coordinates' are generated using the parameter -v."> + <option value="consensus" selected="true">Consensus</option> + <option value="probs" selected="true">Consensus probabilities</option> + <option value="calls" selected="true">Calls to draft</option> + <option value="draft">Draft to consensus</option> + <option value="variants">Variants</option> + <option value="polished">Polished regions in draft coordinates</option> + <option value="log">Log</option> + </param> + </inputs> + <outputs> + <!-- standard --> + <data name="out_consensus" format="fasta" from_work_dir="results/consensus.fasta" label="${tool.name} on ${on_string}: Consensus"> + <filter>'consensus' in out</filter> + </data> + <data name="out_probs" format="h5" from_work_dir="results/consensus_probs.hdf" label="${tool.name} on ${on_string}: Consensus probabilities"> + <filter>'probs' in out</filter> + </data> + <data name="out_calls" format="bam" from_work_dir="results/calls_to_draft.bam" label="${tool.name} on ${on_string}: Calls to draft"> + <filter>'calls' in out</filter> + </data> + <!-- optional with parameter -v --> + <data name="out_draft" format="txt" from_work_dir="results/draft_to_consensus.chain" label="${tool.name} on ${on_string}: Draft to consensus"> + <filter>'draft' in out</filter> + </data> + <data name="out_variants" format="vcf" from_work_dir="results/variants.vcf" label="${tool.name} on ${on_string}: Variants"> + <filter>'variants' in out</filter> + </data> + <data name="out_polished" format="bed" from_work_dir="results/polished_regions_in_draft_coords.bed" label="${tool.name} on ${on_string}: Polished regions in Draft"> + <filter>'polished' in out</filter> + </data> + <!-- optional --> + <data name="out_log" format="txt" label="${tool.name} on ${on_string}: Log" from_work_dir="log.txt"> + <filter>'log' in out</filter> + </data> + </outputs> + <tests> + <!-- #1 default --> + <test expect_num_outputs="3"> + <param name="i" value="basecalls.fastq"/> + <param name="d" value="assembly.fasta"/> + <output name="out_consensus"> + <assert_contents> + <has_n_lines n="2"/> + <has_line line=">4_segment0 4:1.0-3792.0"/> + <has_text_matching expression=".+GTCAGAATCGCGTTCAGCGCGTTTCAGCGGTGCGTACAATT.+"/> + </assert_contents> + </output> + <output name="out_probs"> + <assert_contents> + <has_size value="194782" delta="100"/> + </assert_contents> + </output> + <output name="out_calls"> + <assert_contents> + <has_size value="533139" delta="100"/> + </assert_contents> + </output> + </test> + <!-- #2 --> + <test expect_num_outputs="7"> + <param name="i" value="basecalls.fastq"/> + <param name="d" value="assembly.fasta"/> + <param name="m" value="r941_min_fast_g303"/> + <param name="b" value="99"/> + <param name="out" value="consensus,probs,calls,draft,variants,polished,log"/> + <output name="out_consensus"> + <assert_contents> + <has_n_lines n="65"/> + <has_line line=">4 length=3792 depth=114.52x circular=true"/> + <has_line line="CATCTCTTT"/> + </assert_contents> + </output> + <output name="out_probs"> + <assert_contents> + <has_size value="192710" delta="100"/> + </assert_contents> + </output> + <output name="out_calls"> + <assert_contents> + <has_size value="533163" delta="100"/> + </assert_contents> + </output> + <output name="out_draft"> + <assert_contents> + <has_n_lines n="6"/> + <has_line line="chain 3789 4 3792 + 0 3792 4 3789 + 0 3789 1"/> + <has_line line="1204"/> + </assert_contents> + </output> + <output name="out_polished"> + <assert_contents> + <has_size value="9"/> + </assert_contents> + </output> + <output name="out_variants"> + <assert_contents> + <has_n_lines n="9"/> + <has_line line="##fileformat=VCFv4.1"/> + <has_text_matching expression="4	.+"/> + </assert_contents> + </output> + <output name="out_log"> + <assert_contents> + <has_line line="Polished assembly written to results/consensus.fasta, have a nice day."/> + </assert_contents> + </output> + </test> + </tests> + <help><![CDATA[ +.. class:: infomark + +**What it does** + +@WID@ + +The *medaka_consensus* pipeline performs assembly polishing via neural networks. + +**Input** + +An *assembly* in .fasta format and *basecalls* in .fasta or .fastq format are required. See `Creating a Draft Assembly <https://nanoporetech.github.io/medaka/walkthrough.html#basecalling-and-draft-assembly>`_ for a detailed example of one method of obtaining these. + +**Output** + +- Consensus polished assembly (FASTA) +- Consensus Probabilities (H5/HDF) +- Calls To Draft (BAM) +- Draft To Consensus (chain, TXT) +- Variants: VCF of changes (VCF) +- Polished: BED file of polished regions (BED) + +**References** + +@REFERENCES@ + ]]></help> + <expand macro="citations"/> +</tool>