Mercurial > repos > iuc > medaka_snp
diff macros.xml @ 0:179342c7b86c draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit 4d3dfd4bcb567178107dcfd808ff03f9fec0bdbd
author | iuc |
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date | Wed, 12 Oct 2022 07:43:59 +0000 |
parents | |
children | 630e6aeeb7e8 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Wed Oct 12 07:43:59 2022 +0000 @@ -0,0 +1,188 @@ +<macros> + <token name="@TOOL_VERSION@">1.7.2</token> + <token name="@VERSION_SUFFIX@">0</token> + <token name="@PROFILE@">21.01</token> + <xml name="bio_tools"> + <xrefs> + <xref type="bio.tools">medaka</xref> + </xrefs> + </xml> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">medaka</requirement> + <yield /> + </requirements> + </xml> + <xml name="version_command"> + <version_command>medaka --version</version_command> + </xml> + <xml name="citations"> + <citations> + <citation type="bibtex">@online{medaka, + author = {Oxford Nanopore Technologies Ltd.}, + title = {medaka}, + year = 2020, + url = {https://github.com/nanoporetech/medaka}, + urldate = {2020-05-06} + }</citation> + </citations> + </xml> + + <!-- + command + --> + + <token name="@REF_FASTA@"><![CDATA[ + #if $reference_source.reference_source_selector == 'history': + #if $reference_source.ref_file.ext.endswith(".gz") + gunzip -c '$reference_source.ref_file' > reference.fa && + #else + ln -f -s '$reference_source.ref_file' reference.fa && + #end if + #else: + ln -f -s '$reference_source.ref_file.fields.path' reference.fa && + #end if + ]]></token> + + <!-- + input + --> + + <xml name="b" token_argument="-b"> + <param argument="@ARGUMENT@" type="integer" value="100" min="1" label="Set inference batch size"/> + </xml> + <xml name="model" token_argument="-m" token_label="Select model"> + <param argument="@ARGUMENT@" type="select" label="@LABEL@" help="For best results it is important to specify the correct model, + according to the basecaller used. Medaka models are named to indicate i) the pore type, ii) the sequencing device (MinION + or PromethION), iii) the basecaller variant, and iv) the basecaller version"> + <option value="r103_fast_g507">r103_fast_g507</option> + <option value="r103_fast_snp_g507">r103_fast_snp_g507</option> + <option value="r103_fast_variant_g507">r103_fast_variant_g507</option> + <option value="r103_hac_g507">r103_hac_g507</option> + <option value="r103_hac_snp_g507">r103_hac_snp_g507</option> + <option value="r103_hac_variant_g507">r103_hac_variant_g507</option> + <option value="r103_min_high_g345">r103_min_high_g345</option> + <option value="r103_min_high_g360">r103_min_high_g360</option> + <option value="r103_prom_high_g360">r103_prom_high_g360</option> + <option value="r103_prom_snp_g3210">r103_prom_snp_g3210</option> + <option value="r103_prom_variant_g3210">r103_prom_variant_g3210</option> + <option value="r103_sup_g507">r103_sup_g507</option> + <option value="r103_sup_snp_g507">r103_sup_snp_g507</option> + <option value="r103_sup_variant_g507">r103_sup_variant_g507</option> + <option value="r1041_e82_400bps_fast_g615">r1041_e82_400bps_fast_g615</option> + <option value="r1041_e82_400bps_fast_variant_g615">r1041_e82_400bps_fast_variant_g615</option> + <option value="r1041_e82_400bps_hac_g615">r1041_e82_400bps_hac_g615</option> + <option value="r1041_e82_400bps_hac_variant_g615">r1041_e82_400bps_hac_variant_g615</option> + <option value="r1041_e82_400bps_sup_g615">r1041_e82_400bps_sup_g615</option> + <option value="r1041_e82_400bps_sup_variant_g615">r1041_e82_400bps_sup_variant_g615</option> + <option value="r104_e81_fast_g5015">r104_e81_fast_g5015</option> + <option value="r104_e81_hac_g5015">r104_e81_hac_g5015</option> + <option value="r104_e81_sup_g5015">r104_e81_sup_g5015</option> + <option value="r104_e81_fast_variant_g5015">r104_e81_fast_variant_g5015</option> + <option value="r104_e81_hac_variant_g5015">r104_e81_hac_variant_g5015</option> + <option value="r104_e81_sup_g610">r104_e81_sup_g610</option> + <option value="r104_e81_sup_variant_g610">r104_e81_sup_variant_g610</option> + <option value="r10_min_high_g303">r10_min_high_g303</option> + <option value="r10_min_high_g340">r10_min_high_g340</option> + <option value="r941_e81_fast_variant_g514">r941_e81_fast_variant_g514</option> + <option value="r941_e81_hac_g514">r941_e81_hac_g514</option> + <option value="r941_e81_hac_variant_g514">r941_e81_hac_variant_g514</option> + <option value="r941_e81_sup_g514">r941_e81_sup_g514</option> + <option value="r941_e81_sup_variant_g514">r941_e81_sup_variant_g514</option> + <option value="r941_min_fast_g303">r941_min_fast_g303</option> + <option value="r941_min_fast_g507">r941_min_fast_g507</option> + <option value="r941_min_fast_snp_g507">r941_min_fast_snp_g507</option> + <option value="r941_min_fast_variant_g507">r941_min_fast_variant_g507</option> + <option value="r941_min_hac_g507">r941_min_hac_g507</option> + <option value="r941_min_hac_snp_g507">r941_min_hac_snp_g507</option> + <option value="r941_min_hac_variant_g507">r941_min_hac_variant_g507</option> + <option value="r941_min_high_g303">r941_min_high_g303</option> + <option value="r941_min_high_g330">r941_min_high_g330</option> + <option value="r941_min_high_g340_rle">r941_min_high_g340_rle</option> + <option value="r941_min_high_g344">r941_min_high_g344</option> + <option value="r941_min_high_g351">r941_min_high_g351</option> + <option value="r941_min_high_g360" selected="true">r941_min_high_g360</option> + <option value="r941_min_sup_g507">r941_min_sup_g507</option> + <option value="r941_min_sup_snp_g507">r941_min_sup_snp_g507</option> + <option value="r941_min_sup_variant_g507">r941_min_sup_variant_g507</option> + <option value="r941_prom_fast_g303">r941_prom_fast_g303</option> + <option value="r941_prom_fast_g507">r941_prom_fast_g507</option> + <option value="r941_prom_fast_snp_g507">r941_prom_fast_snp_g507</option> + <option value="r941_prom_fast_variant_g507">r941_prom_fast_variant_g507</option> + <option value="r941_prom_hac_g507">r941_prom_hac_g507</option> + <option value="r941_prom_hac_snp_g507">r941_prom_hac_snp_g507</option> + <option value="r941_prom_hac_variant_g507">r941_prom_hac_variant_g507</option> + <option value="r941_prom_high_g303">r941_prom_high_g303</option> + <option value="r941_prom_high_g330">r941_prom_high_g330</option> + <option value="r941_prom_high_g344">r941_prom_high_g344</option> + <option value="r941_prom_high_g360">r941_prom_high_g360</option> + <option value="r941_prom_high_g4011">r941_prom_high_g4011</option> + <option value="r941_prom_snp_g303">r941_prom_snp_g303</option> + <option value="r941_prom_snp_g322">r941_prom_snp_g322</option> + <option value="r941_prom_snp_g360">r941_prom_snp_g360</option> + <option value="r941_prom_sup_g507">r941_prom_sup_g507</option> + <option value="r941_prom_sup_snp_g507">r941_prom_sup_snp_g507</option> + <option value="r941_prom_sup_variant_g507">r941_prom_sup_variant_g507</option> + <option value="r941_prom_variant_g303">r941_prom_variant_g303</option> + <option value="r941_prom_variant_g322">r941_prom_variant_g322</option> + <option value="r941_prom_variant_g360">r941_prom_variant_g360</option> + <option value="r941_sup_plant_g610">r941_sup_plant_g610</option> + <option value="r941_sup_plant_variant_g610">r941_sup_plant_variant_g610</option> + </param> + </xml> + <xml name="reference"> + <conditional name="reference_source"> + <param name="reference_source_selector" type="select" label="Choose the source for the reference genome"> + <option value="cached">Use a built-in genome</option> + <option value="history">Use a genome from history</option> + </param> + <when value="cached"> + <param name="ref_file" type="select" label="Using reference genome" help="Select genome from the list"> + <options from_data_table="all_fasta"> + <filter type="sort_by" column="2"/> + <validator type="no_options" message="No reference genomes are available"/> + </options> + <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> + </param> + </when> + <when value="history"> + <param name="ref_file" type="data" format="fasta,fasta.gz" label="Use the following dataset as the reference sequence" help="You can upload a FASTA or FASTQ sequence to the history and use it as reference"/> + </when> + </conditional> + </xml> + + <!-- + Help + --> + + <token name="@WID@"><![CDATA[ +*medaka* is a tool suite to create a consensus sequence from nanopore sequencing data. + +This task is performed using neural networks applied from a pileup of individual sequencing reads against a draft assembly. It outperforms graph-based methods operating on basecalled data, and can be competitive with state-of-the-art signal-based methods, whilst being much faster. + ]]></token> + + <token name="@MODELS@"><![CDATA[ + +---- + +.. class:: infomark + +**Models** + +For best results it is important to specify the correct model, -m in the above, according to the basecaller used. Allowed values can be found by running medaka tools list\_models. + +Medaka models are named to indicate i) the pore type, ii) the sequencing device (MinION or PromethION), iii) the basecaller variant, and iv) the basecaller version, with the format: + + :: + + {pore}_{device}_{caller variant}_{caller version} + +For example the model named r941_min_fast_g303 should be used with data from MinION (or GridION) R9.4.1 flowcells using the fast Guppy basecaller version 3.0.3. By contrast the model +r941_prom_hac_g303 should be used with PromethION data and the high accuracy basecaller (termed "hac" in Guppy configuration files). Where a version of Guppy has been used without an exactly corresponding medaka model, the medaka model with the highest version equal to or less than the guppy version should be selected. + + ]]></token> + + <token name="@REFERENCES@"><![CDATA[ +More information are available in the `manual <https://github.com/nanoporetech/medaka/tree/master/docs>`_ and `github <https://github.com/nanoporetech/medaka>`_. + ]]></token> +</macros>