Mercurial > repos > iuc > meme_fimo
diff fimo.xml @ 0:fd522a964017 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e96df94dba60050fa28aaf55b5bb095717a5f260
author | iuc |
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date | Tue, 22 Dec 2015 17:01:51 -0500 |
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children | fdf47de5bdbc |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fimo.xml Tue Dec 22 17:01:51 2015 -0500 @@ -0,0 +1,250 @@ +<tool id="meme_fimo" name="FIMO" version="4.11.0.0"> + <description>- Scan a set of sequences for motifs.</description> + <requirements> + <requirement type="package" version="4.11.0.0">meme</requirement> + </requirements> + <command> + <![CDATA[ + python $__tool_directory__/fimo_wrapper.py + --input_motifs "${input_motifs}" + #if str($fasta_type.fasta_type_selector) == 'history': + --input_fasta "${fasta_type.input_database}" + #else: + --input_fasta "${fasta_type.input_database.fields.path}" + #end if + --options_type $options_type.options_type_selector + #if str($options_type.options_type_selector) == 'advanced': + --alpha "${options_type.alpha}" + #if str($options_type.bgfile_type.bgfile_type_selector) == 'motif_file': + --bgfile "motif-file" + #elif str($options_type.bgfile_type.bgfile_type_selector) == 'bgfile': + --bgfile "${options_type.bgfile_type.bgfile}" + #end if + ${options_type.max_strand} + --max_stored_scores "${options_type.max_stored_scores}" + #if str($options_type.motifs_cond.motifs_selector) == 'no': + #for $motif in $options_type.motifs: + --motif "${motif.motif}" + #end for + #end if + --motif_pseudo "${options_type.motif_pseudo}" + ${options_type.no_qvalue} + ${options_type.norc} + ${options_type.parse_genomic_coord} + #if str($options_type.psp_cond.psp_selector) == 'yes': + --input_psp "${input_psp}" + #end if + #if str($options_type.prior_dist_cond.prior_dist_selector) == 'yes': + --input_prior_dist "${input_prior_dist}" + #end if + ${options_type.qv_thresh} + --thresh ${options_type.thresh} + #end if + --output_path '${html_outfile.files_path}' + --html_output "${html_outfile}" + --interval_output '${interval_outfile}' + --txt_output "${txt_outfile}" + --xml_output "${xml_outfile}" + --gff_output "${gff_outfile}" + ]]> + </command> + <inputs> + <param name="input_motifs" type="data" format="memexml" label="'MEME output' formatted file"/> + <conditional name="fasta_type"> + <param name="fasta_type_selector" type="select" label="Source for sequence to search"> + <option value="cached">Locally Cached sequences</option> + <option value="history" selected="true">Sequences from your history</option> + </param> + <when value="cached"> + <param name="input_database" type="select" label="Genome to search"> + <options from_data_table="all_fasta" /> + </param> + </when> + <when value="history"> + <param format="fasta" name="input_database" type="data" label="Sequences"/> + </when> + </conditional> + <conditional name="options_type"> + <param name="options_type_selector" type="select" label="Options configuration"> + <option value="basic" selected="true">Basic</option> + <option value="advanced">Advanced</option> + </param> + <when value="basic" /> + <when value="advanced"> + <param name="alpha" type="float" value="1.0" min="0" max="1.0" label="Alpha parameter for calculating position specific priors" help="Represents the fraction of all transcription factor binding sites that are binding sites for the TF of interest (must be between 0 and 1)."/> + <conditional name="bgfile_type"> + <param name="bgfile_type_selector" type="select" label="Background file type"> + <option value="default" selected="true">Use frequencies embedded in the application from the non-redundant database</option> + <option value="motif_file">Use frequencies from motif file</option> + <option value="bgfile">Use frequencies from background file</option> + </param> + <when value="motif_file" /> + <when value="default" /> + <when value="bgfile"> + <param name="bgfile" type="data" format="txt" optional="True" label="Background Model" help="File must be in MEME background file format."/> + </when> + </conditional> + <param name="max_strand" label="If matches on both strands at a given position satisfy the output threshold, only report the match for the strand with the higher score" type="boolean" truevalue="--max_strand" falsevalue="" checked="False" help="If the scores are tied, the matching strand is chosen at random. Leave unchecked to report both matches."/> + <param name="max_stored_scores" type="integer" value="100000" label="Maximum number of scores that will be stored" /> + <conditional name="motifs_cond"> + <param name="motifs_selector" type="select" label="Use all motifs in input?"> + <option value="yes" selected="true">Yes</option> + <option value="no">No</option> + </param> + <when value="yes"/> + <when value="no"> + <repeat name="motifs" title="Limit to specified motif"> + <param name="motif" type="text" value="" label="Specify motif by id" /> + </repeat> + </when> + </conditional> + <param name="motif_pseudo" type="float" value="0.1" label="Pseudocount to add to counts in motif matrix" help="A pseudocount to be added to each count in the motif matrix, after first multiplying by the corresponding background frequency"/> + <param name="no_qvalue" label="Do not compute a q-value for each p-value" type="boolean" truevalue="--no_qvalue" falsevalue="" checked="True" help="The q-value calculation is that of Benjamini and Hochberg (1995)."/> + <param name="norc" label="Do not score the reverse complement DNA strand" type="boolean" truevalue="--norc" falsevalue="" checked="False" /> + <param name="parse_genomic_coord" label="Check each sequence header for UCSC style genomic coordinates" type="boolean" truevalue="--parse_genomic_coord" falsevalue="" checked="False" /> + <conditional name="psp_cond"> + <param name="psp_selector" type="select" label="Use position-specific priors?"> + <option value="no" selected="true">No</option> + <option value="yes">Yes</option> + </param> + <when value="no"/> + <when value="yes"> + <param name="input_psp" type="data" format="txt" label="Select dataset containing position specific priors" help="Format must be meme psp or wiggle."/> + </when> + </conditional> + <conditional name="prior_dist_cond"> + <param name="prior_dist_selector" type="select" label="Use binned distribution of priors?"> + <option value="no" selected="true">No</option> + <option value="yes">Yes</option> + </param> + <when value="no"/> + <when value="yes"> + <param name="input_prior_dist" type="data" format="txt" label="Select dataset containing binned distribution of priors"/> + </when> + </conditional> + <param name="qv_thresh" label="Use q-values for the output threshold" type="boolean" truevalue="--qv_thresh" falsevalue="" checked="False" help="Leave unchecked to use p-values for the output threshold."/> + <param name="thresh" type="float" value="1e-4" label="Output threshold for displaying search results" help="Only search results with a p-value less than the threshold will be output. The threshold can be set to use q-values rather than p-values via the option above."/> + </when> + </conditional> + <param name="non_commercial_use" label="I certify that I am not using this tool for commercial purposes." type="boolean" truevalue="NON_COMMERCIAL_USE" falsevalue="COMMERCIAL_USE" checked="False"> + <validator type="expression" message="This tool is only available for non-commercial use.">value == True</validator> + </param> + </inputs> + <outputs> + <data format="html" name="html_outfile" label="${tool.name} on ${on_string} (html)"> + <actions> + <conditional name="fasta_type.fasta_type_selector"> + <when value="cached"> + <action type="metadata" name="dbkey"> + <option type="from_data_table" name="all_fasta" column="1" offset="0"> + <filter type="param_value" column="0" value="seq" keep="True"/> + <filter type="param_value" ref="fasta_type.input_database" column="1"/> + </option> + </action> + </when> + </conditional> + </actions> + </data> + <data format="tabular" name="txt_outfile" label="${tool.name} on ${on_string} (text)"> + <actions> + <conditional name="fasta_type.fasta_type_selector"> + <when value="cached"> + <action type="metadata" name="dbkey"> + <option type="from_data_table" name="all_fasta" column="1" offset="0"> + <filter type="param_value" ref="fasta_type.input_database" column="0"/> + </option> + </action> + </when> + </conditional> + </actions> + </data> + <data format="tabular" name="gff_outfile" label="${tool.name} on ${on_string} (almost-gff)"> + <actions> + <conditional name="fasta_type.fasta_type_selector"> + <when value="cached"> + <action type="metadata" name="dbkey"> + <option type="from_data_table" name="all_fasta" column="1" offset="0"> + <filter type="param_value" ref="fasta_type.input_database" column="0"/> + </option> + </action> + </when> + </conditional> + </actions> + </data> + <data format="cisml" name="xml_outfile" label="${tool.name} on ${on_string} (xml)"> + <actions> + <conditional name="fasta_type.fasta_type_selector"> + <when value="cached"> + <action type="metadata" name="dbkey"> + <option type="from_data_table" name="all_fasta" column="1" offset="0"> + <filter type="param_value" ref="fasta_type.input_database" column="0"/> + </option> + </action> + </when> + </conditional> + </actions> + </data> + <data format="interval" name="interval_outfile" label="${tool.name} on ${on_string} (interval)"> + <actions> + <conditional name="fasta_type.fasta_type_selector"> + <when value="cached"> + <action type="metadata" name="dbkey"> + <option type="from_data_table" name="all_fasta" column="1" offset="0"> + <filter type="param_value" ref="fasta_type.input_database" column="0"/> + </option> + </action> + </when> + </conditional> + </actions> + </data> + </outputs> + <tests> + <test> + <param name="input_motifs" value="meme_output_xml_1.xml" ftype="memexml"/> + <param name="fasta_type_selector" value="history"/> + <param name="input_database" value="phiX.fasta" ftype="fasta"/> + <param name="options_type_selector" value="basic"/> + <param name="non_commercial_use" value="True"/> + <output name="html_outfile" file="fimo_output_html_1.html" compare="contains"/> + <output name="txt_outfile" file="fimo_output_txt_1.txt" compare="contains"/> + <output name="gff_outfile" file="fimo_output_almost-gff_1.txt" compare="contains"/> + <output name="xml_outfile" file="fimo_output_xml_1.xml" lines_diff="8"/> + <output name="interval_outfile" file="fimo_output_interval_1.txt" compare="contains"/> + </test> + <test> + <param name="input_motifs" value="meme_output_xml_1.xml" ftype="memexml"/> + <param name="fasta_type_selector" value="history"/> + <param name="input_database" value="phiX.fasta" ftype="fasta"/> + <param name="options_type_selector" value="advanced"/> + <param name="non_commercial_use" value="True"/> + <output name="html_outfile" file="fimo_output_html_2.html" compare="contains"/> + <output name="txt_outfile" file="fimo_output_txt_2.txt" compare="contains"/> + <output name="gff_outfile" file="fimo_output_almost-gff_2.txt" compare="contains"/> + <output name="xml_outfile" file="fimo_output_xml_2.xml" lines_diff="8"/> + <output name="interval_outfile" file="fimo_output_interval_2.txt" compare="contains"/> + </test> + </tests> + <help> + +.. class:: warningmark + +**WARNING: This tool is only available for non-commercial use. Use for educational, research and non-profit purposes is permitted. +Before using, be sure to review, agree, and comply with the license.** + +FIMO scans a sequence database for individual matches to each of the motifs you provide (sample output for motifs and sequences). +The name FIMO stands for 'Find Individual Motif Occurrences'. The program searches a database of sequences for occurrences of +known motifs, treating each motif independently. Motifs must be in MEME Motif Format. You can define the statistical threshold +(p-value) for motifs and whether FIMO scans just the given sequences or their reverse complements (where applicable), too. + +.. class:: infomark + +For detailed information on FIMO, click here_, or view the license_. + +.. _here: http://meme-suite.org/doc/fimo.html?man_type=web +.. _license: http://meme-suite.org/doc/copyright.html?man_type=web + + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btr064</citation> + </citations> +</tool>