Mercurial > repos > iuc > metaphlan
annotate formatoutput.py @ 7:ec368db926f4 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 42f32d4c093fc3b8d2604c635e1f862fee695a05
author | iuc |
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date | Wed, 29 Mar 2023 08:00:24 +0000 |
parents | 6dee4abadccb |
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rev | line source |
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1
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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1 #!/usr/bin/env python |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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2 # -*- coding: utf-8 -*- |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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3 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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4 import argparse |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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5 import re |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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6 from pathlib import Path |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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7 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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8 taxo_level = { |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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9 'k': 'kingdom', |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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10 'p': 'phylum', |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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11 'c': 'class', |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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12 'o': 'order', |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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13 'f': 'family', |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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14 'g': 'genus', |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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15 's': 'species', |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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16 't': 'strains'} |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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17 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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18 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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19 def split_levels(metaphlan_output_fp, out_dp, legacy_output): |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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20 ''' |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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21 Split default MetaPhlAn into a report for each taxonomic level |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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22 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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23 :param metaphlan_output_fp: Path default MetaPhlAn output |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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24 :param out_dp: Path to output directory |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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25 :param legacy_output: Boolean for legacy output |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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26 ''' |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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27 # prepare output files |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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28 abund_f = { |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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29 'k': open(out_dp / Path('kingdom'), 'w'), |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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30 'p': open(out_dp / Path('phylum'), 'w'), |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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31 'c': open(out_dp / Path('class'), 'w'), |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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32 'o': open(out_dp / Path('order'), 'w'), |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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33 'f': open(out_dp / Path('family'), 'w'), |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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34 'g': open(out_dp / Path('genus'), 'w'), |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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35 's': open(out_dp / Path('species'), 'w'), |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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36 't': open(out_dp / Path('strains'), 'w') |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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37 } |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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38 for level in abund_f: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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39 abund_f[level].write("%s\t" % taxo_level[level]) |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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40 if not legacy_output: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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41 abund_f[level].write("%s_id\t" % taxo_level[level]) |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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42 abund_f[level].write("abundance\n") |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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43 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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44 levels_number = len(taxo_level) |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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45 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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46 with open(metaphlan_output_fp, 'r') as metaphlan_output_f: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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47 with open(out_dp / Path('all'), 'w') as all_level_f: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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48 # write header in all leve file |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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49 for level in ['k', 'p', 'c', 'o', 'f', 'g', 's', 't']: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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50 all_level_f.write("%s\t" % taxo_level[level]) |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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51 if not legacy_output: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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52 all_level_f.write("%s_id\t" % taxo_level[level]) |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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53 all_level_f.write("abundance\n") |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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54 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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55 # parse metaphlan file |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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56 for line in metaphlan_output_f.readlines(): |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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57 # skip headers |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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58 if line.startswith("#"): |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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59 continue |
5
6dee4abadccb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit f3a89af137b13715f9fb13383577aceb2c445ce6
iuc
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60 |
6dee4abadccb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit f3a89af137b13715f9fb13383577aceb2c445ce6
iuc
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61 # skip UNKNOWN (v3) or UNCLASSIFIED (v4) lines in predicted taxon relative abundances |
6dee4abadccb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit f3a89af137b13715f9fb13383577aceb2c445ce6
iuc
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62 if "UNKNOWN" in line or 'UNCLASSIFIED' in line: |
2
a92a632c4d9b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit f1c6f4fe1e572ace84cf9106bc253603f55aac55"
iuc
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63 continue |
5
6dee4abadccb
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit f3a89af137b13715f9fb13383577aceb2c445ce6
iuc
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64 |
1
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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65 # spit lines |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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66 split_line = line[:-1].split('\t') |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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67 taxo_n = split_line[0].split('|') |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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68 if legacy_output: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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69 abundance = split_line[1] |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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70 else: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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71 taxo_id = split_line[1].split('|') |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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72 abundance = split_line[2] |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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73 |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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74 # get taxon name and ids |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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75 for i in range(len(taxo_n)): |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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76 taxo = taxo_n[i].split('__')[1] |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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77 taxo = taxo.replace("_", " ") |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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78 all_level_f.write("%s\t" % taxo) |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
parents:
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79 if not legacy_output: |
b89b0765695d
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 2b87bc7417360e2b2c9ec0605d475909f6f0482f"
iuc
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80 all_level_f.write("%s\t" % taxo_id[i]) |
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81 |
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82 # if not all taxon levels |
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83 for i in range(len(taxo_n), levels_number): |
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84 all_level_f.write('\t') |
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85 |
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86 all_level_f.write("%s\n" % abundance) |
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87 |
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88 # write |
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89 last_taxo_level = taxo_n[-1].split('__') |
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90 taxo = last_taxo_level[1].replace("_", " ") |
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91 level = last_taxo_level[0] |
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92 abund_f[level].write("%s\t" % taxo) |
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93 if not legacy_output: |
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94 abund_f[level].write("%s\t" % taxo_id[-1]) |
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95 abund_f[level].write("%s\n" % abundance) |
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96 |
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97 # close files |
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98 for taxo_level_f in abund_f: |
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99 abund_f[taxo_level_f].close() |
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100 |
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101 |
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102 def format_for_krona(metaphlan_output_fp, krona_out_fp): |
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103 ''' |
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104 Split default MetaPhlAn into a report for each taxonomic levKRONAel |
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105 |
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106 :param metaphlan_output_fp: Path default MetaPhlAn output |
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107 :param krona_out: Path to output file for Krona |
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108 ''' |
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109 re_replace = re.compile(r"\w__") |
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110 re_bar = re.compile(r"\|") |
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111 re_underscore = re.compile(r"_") |
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112 |
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113 with open(metaphlan_output_fp, 'r') as metaphlan_output_f: |
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114 with open(krona_out_fp, 'w') as krona_out_f: |
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115 for line in metaphlan_output_f.readlines(): |
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116 if "s__" in line: |
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117 x = line.rstrip().split('\t') |
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118 lineage = re.sub(re_bar, '', x[0]) |
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119 lineage = re.sub(re_replace, '\t', lineage) |
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120 lineage = re.sub(re_underscore, ' ', lineage) |
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121 krona_out_f.write("%s\t%s\n" % (x[-1], lineage)) |
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122 |
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123 |
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124 if __name__ == '__main__': |
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125 parser = argparse.ArgumentParser(description='Format MetaPhlAn output') |
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126 subparsers = parser.add_subparsers(dest='function') |
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127 # split_levels |
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128 split_levels_parser = subparsers.add_parser('split_levels', help='Split default MetaPhlAn into a report for each taxonomic level') |
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129 split_levels_parser.add_argument('--metaphlan_output', help="Path to default MetaPhlAn output") |
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130 split_levels_parser.add_argument('--outdir', help="Path to output directory") |
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131 split_levels_parser.add_argument('--legacy-output', dest='legacy_output', action='store_true', help="Old MetaPhlAn2 two columns output") |
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132 split_levels_parser.set_defaults(legacy_output=False) |
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133 # format_for_krona |
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134 format_for_krona_parser = subparsers.add_parser('format_for_krona', help='Split default MetaPhlAn into a report for each taxonomic level') |
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135 format_for_krona_parser.add_argument('--metaphlan_output', help="Path to default MetaPhlAn output") |
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136 format_for_krona_parser.add_argument('--krona_output', help="Path to Krona output directory") |
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137 |
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138 args = parser.parse_args() |
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139 |
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140 if args.function == 'split_levels': |
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141 split_levels( |
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142 Path(args.metaphlan_output), |
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143 Path(args.outdir), |
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144 args.legacy_output) |
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145 elif args.function == 'format_for_krona': |
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146 format_for_krona( |
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147 Path(args.metaphlan_output), |
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148 Path(args.krona_output)) |