annotate metaphlan.xml @ 0:f5df500fcc3c draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
author iuc
date Mon, 19 Apr 2021 20:56:20 +0000
parents
children b89b0765695d
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
0
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
1 <tool id="metaphlan" name="MetaPhlAn" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
2 <description>to profile the composition of microbial communities</description>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
3 <macros>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
4 <import>macros.xml</import>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
5 </macros>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
6 <expand macro="edam_ontology"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
7 <expand macro="requirements"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
8 <version_command>metaphlan -v</version_command>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
9 <command detect_errors="aggressive"><![CDATA[
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
10 #if $inputs.in.selector == "raw"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
11 #if $inputs.in.raw_in.selector == "single"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
12 #set full_ext=$inputs.in.raw_in.in.datatype.file_ext
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
13 #if $full_ext.endswith("gz")
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
14 #set $file_path="in"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
15 zcat '$inputs.in.raw_in.in' > '$file_path'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
16 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
17 #else if $full_ext.endswith("bz2")
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
18 #set $file_path="in"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
19 bzcat '$inputs.in.raw_in.in' > '$file_path'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
20 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
21 #else
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
22 #set $file_path=$inputs.in.raw_in.in
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
23 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
24 #else if $inputs.in.raw_in.selector == "multiple"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
25 #set full_ext=$inputs.in.raw_in.in[0].datatype.file_ext
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
26 #set file_path=""
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
27 #set sep=""
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
28 #for $i, $f in enumerate($inputs.in.raw_in.in)
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
29 #if $f.datatype.file_ext != $full_ext
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
30 echo "Different datatypes for input files"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
31 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
32 exit 1
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
33 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
34 #if $full_ext.endswith("gz")
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
35 #set fp="input_%s" % ($i)
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
36 zcat '$f' > '$fp'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
37 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
38 #else if $full_ext.endswith("bz2")
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
39 #set fp="input_%s" % ($i)
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
40 bzcat '$f' > '$fp'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
41 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
42 #else
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
43 #set fp=$f
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
44 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
45 #set $file_path+="%s%s" % ($sep, $fp)
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
46 #set $sep=","
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
47 #end for
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
48 #else if $inputs.in.raw_in.selector == "paired"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
49 #set full_ext=$inputs.in.raw_in.in_f.datatype.file_ext
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
50 #if $full_ext != $inputs.in.raw_in.in_r.datatype.file_ext
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
51 echo "Different datatypes for input paired-end files"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
52 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
53 exit 1
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
54 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
55 #if $full_ext.endswith("gz")
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
56 zcat '$inputs.in.raw_in.in_f' > 'in_f'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
57 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
58 zcat '$inputs.in.raw_in.in_r' > 'in_r'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
59 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
60 #set file_path="in_f,in_r"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
61 #else if $full_ext.endswith("bz2")
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
62 bzcat '$inputs.in.raw_in.in_f' > 'in_f'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
63 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
64 bzcat '$inputs.in.raw_in.in_r' > 'in_r'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
65 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
66 #set file_path="in_f,in_r"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
67 #else
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
68 #set file_path="%s,%s" % ($inputs.in.raw_in.in_f,$inputs.in.raw_in.in_r)
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
69 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
70 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
71
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
72 #if $full_ext.startswith("fastq")
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
73 #set ext='fastq'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
74 #else if $full_ext.startswith("fasta") and $full_ext.endswith(("gz","bz2"))
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
75 #set ext='fasta'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
76 #else
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
77 #set ext=$full_ext
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
78 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
79 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
80
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
81 #if $inputs.db.db_selector == "history"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
82 mkdir 'ref_db'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
83 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
84 bowtie2-build '$inputs.db.bowtie2db' 'ref_db/custom_db'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
85 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
86 python '$__tool_directory__/customizemetadata.py'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
87 transform_json_to_pkl
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
88 --json '$inputs.db.mpa_pkl'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
89 --pkl 'ref_db/custom_db.pkl'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
90 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
91 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
92
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
93 metaphlan
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
94 #if $inputs.in.selector == "raw"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
95 '$file_path'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
96 --input_type '$ext'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
97 --read_min_len $inputs.in.read_min_len
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
98 --bt2_ps '$inputs.in.mapping.bt2_ps'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
99 --min_mapq_val $inputs.in.mapping.min_mapq_val
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
100 #else
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
101 '$inputs.in.in'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
102 --input_type '$inputs.in.selector'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
103 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
104 #if $inputs.db.db_selector == "cached"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
105 --bowtie2db '$inputs.db.cached_db.fields.path'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
106 --index '$inputs.db.cached_db.fields.dbkey'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
107 #else
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
108 --bowtie2db 'ref_db/'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
109 --index 'custom_db'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
110 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
111 -t '$analysis.analysis_type.t'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
112 #if $analysis.analysis_type.t == "rel_ab" or $analysis.analysis_type.t == "rel_ab_w_read_stats"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
113 --tax_lev '$analysis.analysis_type.tax_lev'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
114 #else if $analysis.analysis_type.t == "clade_specific_strain_tracker"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
115 --clade '$analysis.analysis_type.clade'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
116 #if str($analysis.analysis_type.min_ab) != ''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
117 --min_ab $analysis.analysis_type.min_ab
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
118 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
119 #else if $analysis.analysis_type.t == "marker_ab_table" and str($analysis.analysis_type.nreads) != ''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
120 --nreads $$analysis.analysis_type.nreads
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
121 #else if $analysis.analysis_type.t == "marker_pres_table" and str($analysis.analysis_type.pres_th) != ''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
122 --pres_th $analysis.analysis_type.pres_th
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
123 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
124 --min_cu_len $analysis.min_cu_len
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
125 #if str($analysis.min_alignment_len) != ''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
126 --min_alignment_len $analysis.min_alignment_len
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
127 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
128 #if 'add_viruses' in $analysis.organism_profiling
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
129 --add_viruses
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
130 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
131 #if 'ignore_eukaryotes' in $analysis.organism_profiling
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
132 --ignore_eukaryotes
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
133 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
134 #if 'ignore_bacteria' in $analysis.organism_profiling
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
135 --ignore_bacteria
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
136 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
137 #if 'ignore_archaea' in $analysis.organism_profiling
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
138 --ignore_archaea
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
139 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
140 --stat_q $analysis.stat_q
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
141 --perc_nonzero $analysis.perc_nonzero
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
142 #if $analysis.ignore_markers
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
143 --ignore_markers '$analysis.ignore_markers'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
144 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
145 $analysis.avoid_disqm
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
146 --sample_id_key '$out.sample_id_key'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
147 --sample_id '$out.sample_id'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
148 $out.use_group_representative
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
149 $out.legacy_output
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
150 $out.CAMI_format_output
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
151 $out.unknown_estimation
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
152 -o '$output_file'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
153 --bowtie2out 'bowtie2out'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
154 -s '$sam_output_file'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
155 --biom '$biom_output_file'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
156 --nproc \${GALAXY_SLOTS:-4}
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
157
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
158 #if $inputs.in.selector == "raw"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
159 &&
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
160 mv 'bowtie2out' '$bowtie2out'
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
161 #end if
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
162 ]]></command>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
163 <inputs>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
164 <section name="inputs" title="Inputs" expanded="true">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
165 <conditional name="in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
166 <param name="selector" type="select" label="Input(s)">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
167 <option value="raw" selected="true">Fasta/FastQ file(s) with metagenomic reads</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
168 <option value="sam">Externally BowTie2-mapped SAM file</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
169 <option value="bowtie2out">Intermediary mapping file of the metagenome generated by a previous MetaPhlAn run</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
170 </param>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
171 <when value="raw">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
172 <conditional name="raw_in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
173 <param name="selector" type="select" label="Fasta/FastQ file(s) with metagenomic reads">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
174 <option value="single" selected="true">One single-end file</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
175 <option value="multiple">Multiple single-end files</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
176 <option value="paired">Paired-end files</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
177 </param>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
178 <when value="single">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
179 <param name="in" type="data" format="@FILE_FORMATS@" label="Single-end Fasta/FastQ file with metagenomic reads"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
180 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
181 <when value="multiple">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
182 <param name="in" type="data" format="@FILE_FORMATS@" multiple="true" label="Single-end Fasta/FastQ files with metagenomic reads"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
183 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
184 <when value="paired">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
185 <param name="in_f" type="data" format="@FILE_FORMATS@" label="Forward paired-end Fasta/FastQ file with metagenomic reads"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
186 <param name="in_r" type="data" format="@FILE_FORMATS@" label="Reverse paired-end Fasta/FastQ file with metagenomic reads"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
187 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
188 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
189 <param argument="--read_min_len" type="integer" value="70" label="Minimum length of the reads to be considered when parsing the input file"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
190 <section name="mapping" title="Mapping" expanded="true">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
191 <param argument="--bt2_ps" type="select" label="Presets options for BowTie2" help="Applied only with FASTA files">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
192 <option value="sensitive">Sensitive</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
193 <option value="very-sensitive" selected="true">Very sensitive</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
194 <option value="sensite-local">Sensitive local</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
195 <option value="very-sensite-local">Very sensitive local</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
196 </param>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
197 <param argument="--min_mapq_val" type="integer" value="5" label="Minimum mapping quality value (MAPQ)"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
198 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
199 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
200 <when value="sam">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
201 <param name="in" type="data" format="sam" label="Externally BowTie2-mapped SAM file" help="BowTie2 needs to be used first to map metagenom reads"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
202 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
203 <when value="bowtie2out">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
204 <param name="in" type="data" format="tabular" label="Intermediary mapping file of the metagenome generated by a previous MetaPhlAn run"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
205 help="File needs to be generated with MetaPhlAn versions >3.0"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
206 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
207 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
208 <conditional name="db">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
209 <param name="db_selector" type="select" label="Database with clade-specific marker genes">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
210 <option value="cached" selected="true">Locally cached</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
211 <option value="history">From history</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
212 </param>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
213 <when value="cached">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
214 <param name="cached_db" label="Cached database with clade-specific marker genes" type="select">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
215 <options from_data_table="metaphlan_database">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
216 <validator message="No MetaPhlAn database is available" type="no_options" />
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
217 </options>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
218 </param>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
219 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
220 <when value="history">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
221 <param argument="--bowtie2db" type="data" format="fasta" label="Database with clade-specific marker genes from history"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
222 <param argument="--mpa_pkl" type="data" format="json" label="Metadata associate to the database with clade-specific marker genes from history"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
223 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
224 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
225 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
226 <section name="analysis" title="Analysis" expanded="true">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
227 <conditional name="analysis_type">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
228 <param argument="-t" type="select" label="Type of analysis to perform">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
229 <option value="rel_ab" selected="true">rel_ab: Profiling a metagenomes in terms of relative abundances</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
230 <option value="rel_ab_w_read_stats">rel_ab_w_read_stats: Profiling a metagenomes in terms of relative abundances and estimate the number of reads comming from each clade</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
231 <option value="reads_map">reads_map: Mapping from reads to clades (only reads hitting a marker)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
232 <option value="clade_profiles">clade_profiles: Normalized marker counts for clades with at least a non-null marker</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
233 <option value="clade_specific_strain_tracker">clade_specific_strain_tracker: List of markers present for a specific clade and all its subclades</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
234 <option value="marker_ab_table">marker_ab_table: Normalized marker counts (only when > 0.0 and normalized by metagenome size if number of reads is specified)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
235 <option value="marker_counts">marker_counts: Non-normalized marker counts (use with extreme caution)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
236 <option value="marker_pres_table">marker_pres_table: List of markers present in the sample (threshold at 1.0 if not differently specified with --pres_th</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
237 </param>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
238 <when value="rel_ab">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
239 <expand macro="tax_lev"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
240 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
241 <when value="rel_ab_w_read_stats">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
242 <expand macro="tax_lev"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
243 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
244 <when value="reads_map"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
245 <when value="clade_profiles"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
246 <when value="clade_specific_strain_tracker">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
247 <param argument="--clade" type="text" value="" label="Clade for which to extract list of markers present"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
248 help="Markers are also extracted for subclades" />
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
249 <param argument="--min_ab" type="float" optional="true" label="The minimum percentage abundance for the clade"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
250 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
251 <when value="marker_ab_table">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
252 <param argument="--nreads" type="integer" optional="true" label="Total number of reads in the original metagenome"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
253 help="It is used for normalizing the length-normalized counts with the metagenome size as well. No normalization applied if the value is not specified"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
254 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
255 <when value="marker_counts"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
256 <when value="marker_pres_table">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
257 <param argument="--pres_th" type="integer" optional="true" label="Threshold for calling a marker present"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
258 </when>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
259 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
260 <param argument="--min_cu_len" type="integer" value="2000"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
261 label="Minimum total nucleotide length for the markers in a clade for estimating the abundance without considering sub-clade abundances"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
262 <param argument="--min_alignment_len" type="integer" optional="true"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
263 label="Sam records for aligned reads with the longest subalignment length smaller than this threshold will be discarded."/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
264 <param name="organism_profiling" type="select" multiple="true" optional="true" label="Organisms to profile">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
265 <option value="add_viruses" selected="true">Profile viral organisms (add_viruses)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
266 <option value="ignore_eukaryotes">Ignore eukaryotic organisms (ignore_eukaryotes)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
267 <option value="ignore_bacteria">Ignore bacteria organisms (ignore_bacteria)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
268 <option value="ignore_archaea">Ignore archea organisms (ignore_archaea)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
269 </param>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
270 <param argument="--stat" type="select" label="Statistical approach for converting marker abundances into clade abundances">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
271 <option value="avg_g">avg_g: Clade global (i.e. normalizing all markers together) average (avg_g)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
272 <option value="avg_l">avg_l: Average of length-normalized marker counts</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
273 <option value="tavg_g" selected="true">tavg_g: Truncated clade global average at --stat_q quantile</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
274 <option value="tavg_l">tavg_l: Truncated average of length-normalized marker counts (at --stat_q)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
275 <option value="wavg_g">wavg_g: Winsorized clade global average (at --stat_q)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
276 <option value="wavg_l">wavg_l: Winsorized average of length-normalized marker counts (at --stat_q)</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
277 <option value="med">med: Median of length-normalized marker counts</option>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
278 </param>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
279 <param argument="--stat_q" type="float" value="0.2" label="Quantile value for the robust average"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
280 <param argument="--perc_nonzero" type="float" value="0.33" label="Percentage of markers with a non zero relative abundance for misidentify a species"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
281 <param argument="--ignore_markers" type="data" format="txt,tabular" optional="true" label="File containing a list of markers to ignore" help="One marker per line"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
282 <param argument="--avoid_disqm" type='boolean' checked="true" truevalue='--avoid_disqm' falsevalue=''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
283 label="Deactivate the procedure of disambiguating the quasi-markers based on the marker abundance pattern found in the sample?"
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
284 help="It is generally recommended to keep the disambiguation procedure in order to minimize false positives"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
285 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
286 <section name="out" title="Outputs" expanded="true">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
287 <param argument="--sample_id_key" type="text" value="SampleID" label="Sample ID key for this analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
288 <param argument="--sample_id" type="text" value="Metaphlan_Analysis" label="Sample ID for this analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
289 <param argument="--use_group_representative" type='boolean' checked="false" truevalue='--use_group_representative' falsevalue=''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
290 label="Use a species as representative for species groups?"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
291 <param name="legacy_output" argument="--legacy-output" type='boolean' checked="false" truevalue='--legacy-output' falsevalue=''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
292 label="Old MetaPhlAn2 two columns output?"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
293 <param argument="--CAMI_format_output" type='boolean' checked="false" truevalue='--CAMI_format_output' falsevalue=''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
294 label="Report the profiling using the CAMI output format?"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
295 <param argument="--unknown_estimation" type='boolean' checked="false" truevalue='--unknown_estimation' falsevalue=''
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
296 label="Scale relative abundances to the number of reads mapping to known clades in order to estimate unknowness?"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
297 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
298 </inputs>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
299 <outputs>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
300 <data name="output_file" format="tabular" label="${tool.name} on ${on_string}: Predicted taxon relative abundances" />
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
301 <data name="bowtie2out" format="tabular" label="${tool.name} on ${on_string}: Bowtie2 output">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
302 <filter>inputs['in']['selector'] == "raw"</filter>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
303 </data>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
304 <data name="sam_output_file" format="sam" label="${tool.name} on ${on_string}: SAM file">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
305 <filter>inputs['in']['selector'] == "raw"</filter>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
306 </data>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
307 <data name="biom_output_file" format="biom1" label="${tool.name} on ${on_string}: BIOM file" />
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
308 </outputs>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
309 <tests>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
310 <test expect_num_outputs="4">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
311 <section name="inputs">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
312 <conditional name="in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
313 <param name="selector" value="raw"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
314 <conditional name="raw_in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
315 <!-- Single GZ file -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
316 <param name="selector" value="single"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
317 <param name="in" value="SRS014464-Anterior_nares.fasta.gz"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
318 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
319 <param name="read_min_len" value="70"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
320 <section name="mapping">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
321 <param name="bt2_ps" value="sensitive"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
322 <param name="min_mapq_val" value="5"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
323 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
324 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
325 <conditional name="db">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
326 <!-- Cached db -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
327 <param name="db_selector" value="cached"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
328 <param name="cached_db" value="test-db-20210409"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
329 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
330 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
331 <section name="analysis">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
332 <param name="min_cu_len" value="2000"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
333 <param name="organism_profiling" value="add_viruses"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
334 <param name="stat" value="avg_g"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
335 <param name="stat_q" value="0.2"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
336 <param name="perc_nonzero" value="0.33"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
337 <param name="avoid_disqm" value="true"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
338 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
339 <section name="out">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
340 <param name="sample_id_key" value="SampleID"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
341 <param name="sample_id" value="Metaphlan_Analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
342 <param name="use_group_representative" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
343 <param name="legacy_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
344 <param name="CAMI_format_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
345 <param name="unknown_estimation" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
346 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
347 <output name="output_file" ftype="tabular" file="SRS014464-Anterior_nares-abundances.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
348 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
349 <has_text text="k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Corynebacteriales|f__Corynebacteriaceae|g__Corynebacterium|s__Corynebacterium_accolens"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
350 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
351 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
352 <output name="bowtie2out" ftype="tabular" file="SRS014464-Anterior_nares-bowtie2out.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
353 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
354 <has_text text="HWI-EAS109_102883399:3:104:7342:14360/1"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
355 <has_text text="37637__U2I1U8__N579_01580"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
356 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
357 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
358 <output name="sam_output_file" ftype="sam" file="SRS014464-Anterior_nares.sam" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
359 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
360 <has_text text="SN:13076__A0A2I1PE66__CYJ72_10760"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
361 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
362 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
363 <output name="biom_output_file" ftype="biom1" file="SRS014464-Anterior_nares.biom" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
364 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
365 <has_text text="k__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Pseudomonadales|f__Moraxellaceae|g__Moraxella|s__Moraxella_lacunata"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
366 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
367 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
368 </test>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
369 <test expect_num_outputs="4">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
370 <section name="inputs">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
371 <conditional name="in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
372 <param name="selector" value="raw"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
373 <conditional name="raw_in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
374 <!-- Multiple GZ file -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
375 <param name="selector" value="multiple"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
376 <param name="in" value="SRS014464-Anterior_nares.fasta.gz,SRS014464-Anterior_nares.fasta.gz"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
377 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
378 <param name="read_min_len" value="70"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
379 <section name="mapping">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
380 <param name="bt2_ps" value="sensitive"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
381 <param name="min_mapq_val" value="5"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
382 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
383 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
384 <conditional name="db">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
385 <!-- Local db -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
386 <param name="db_selector" value="history"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
387 <param name="bowtie2db" value="test-db.fasta"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
388 <param name="mpa_pkl" value="test-db.json"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
389 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
390 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
391 <section name="analysis">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
392 <param name="min_cu_len" value="2000"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
393 <param name="organism_profiling" value="add_viruses"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
394 <param name="stat" value="avg_g"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
395 <param name="stat_q" value="0.2"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
396 <param name="perc_nonzero" value="0.33"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
397 <param name="avoid_disqm" value="true"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
398 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
399 <section name="out">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
400 <param name="sample_id_key" value="SampleID"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
401 <param name="sample_id" value="Metaphlan_Analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
402 <param name="use_group_representative" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
403 <param name="legacy_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
404 <param name="CAMI_format_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
405 <param name="unknown_estimation" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
406 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
407 <output name="output_file" ftype="tabular" file="SRS014464-Anterior_nares-abundances.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
408 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
409 <has_text text="k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Corynebacteriales|f__Corynebacteriaceae|g__Corynebacterium|s__Corynebacterium_accolens"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
410 <has_text text="relative_abundance"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
411 <has_text text="NCBI_tax_id"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
412 <has_text text="clade_name"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
413 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
414 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
415 <output name="bowtie2out" ftype="tabular" file="SRS014464-Anterior_nares-two-inputs-bowtie2out.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
416 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
417 <has_text text="HWI-EAS109_102883399:3:104:7342:14360/1"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
418 <has_text text="37637__U2I1U8__N579_01580"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
419 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
420 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
421 <output name="sam_output_file" ftype="sam" file="SRS014464-Anterior_nares-two-inputs.sam" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
422 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
423 <has_text text="SN:13076__A0A2I1PE66__CYJ72_10760"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
424 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
425 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
426 <output name="biom_output_file" ftype="biom1" file="SRS014464-Anterior_nares.biom" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
427 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
428 <has_text text="k__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Pseudomonadales|f__Moraxellaceae|g__Moraxella|s__Moraxella_lacunata"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
429 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
430 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
431 </test>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
432 <test expect_num_outputs="4">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
433 <section name="inputs">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
434 <conditional name="in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
435 <param name="selector" value="raw"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
436 <conditional name="raw_in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
437 <!-- Paired GZ file -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
438 <param name="selector" value="paired"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
439 <param name="in_f" value="SRS014464-Anterior_nares.fasta.gz"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
440 <param name="in_r" value="SRS014464-Anterior_nares.fasta.gz"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
441 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
442 <param name="read_min_len" value="70"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
443 <section name="mapping">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
444 <param name="bt2_ps" value="sensitive"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
445 <param name="min_mapq_val" value="5"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
446 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
447 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
448 <conditional name="db">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
449 <!-- Cached db -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
450 <param name="db_selector" value="cached"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
451 <param name="cached_db" value="test-db-20210409"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
452 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
453 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
454 <section name="analysis">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
455 <param name="min_cu_len" value="2000"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
456 <param name="organism_profiling" value="add_viruses"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
457 <param name="stat" value="avg_g"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
458 <param name="stat_q" value="0.2"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
459 <param name="perc_nonzero" value="0.33"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
460 <param name="avoid_disqm" value="true"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
461 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
462 <section name="out">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
463 <param name="sample_id_key" value="SampleID"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
464 <param name="sample_id" value="Metaphlan_Analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
465 <param name="use_group_representative" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
466 <param name="legacy_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
467 <param name="CAMI_format_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
468 <param name="unknown_estimation" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
469 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
470 <output name="output_file" ftype="tabular" file="SRS014464-Anterior_nares-abundances.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
471 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
472 <has_text text="k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Corynebacteriales|f__Corynebacteriaceae|g__Corynebacterium|s__Corynebacterium_accolens"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
473 <has_text text="relative_abundance"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
474 <has_text text="NCBI_tax_id"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
475 <has_text text="clade_name"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
476 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
477 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
478 <output name="bowtie2out" ftype="tabular" file="SRS014464-Anterior_nares-two-inputs-bowtie2out.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
479 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
480 <has_text text="HWI-EAS109_102883399:3:104:7342:14360/1"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
481 <has_text text="37637__U2I1U8__N579_01580"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
482 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
483 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
484 <output name="sam_output_file" ftype="sam" file="SRS014464-Anterior_nares-two-inputs.sam" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
485 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
486 <has_text text="SN:13076__A0A2I1PE66__CYJ72_10760"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
487 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
488 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
489 <output name="biom_output_file" ftype="biom1" file="SRS014464-Anterior_nares.biom" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
490 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
491 <has_text text="k__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Pseudomonadales|f__Moraxellaceae|g__Moraxella|s__Moraxella_lacunata"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
492 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
493 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
494 </test>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
495 <test expect_num_outputs="2">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
496 <section name="inputs">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
497 <conditional name="in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
498 <!-- SAM -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
499 <param name="selector" value="sam"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
500 <param name="in" value="SRS014464-Anterior_nares.sam"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
501 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
502 <conditional name="db">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
503 <!-- Cached db -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
504 <param name="db_selector" value="cached"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
505 <param name="cached_db" value="test-db-20210409"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
506 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
507 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
508 <section name="analysis">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
509 <param name="min_cu_len" value="2000"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
510 <param name="organism_profiling" value="add_viruses"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
511 <param name="stat" value="avg_g"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
512 <param name="stat_q" value="0.2"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
513 <param name="perc_nonzero" value="0.33"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
514 <param name="avoid_disqm" value="true"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
515 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
516 <section name="out">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
517 <param name="sample_id_key" value="SampleID"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
518 <param name="sample_id" value="Metaphlan_Analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
519 <param name="use_group_representative" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
520 <param name="legacy_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
521 <param name="CAMI_format_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
522 <param name="unknown_estimation" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
523 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
524 <output name="output_file" ftype="tabular" file="SRS014464-Anterior_nares-abundances.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
525 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
526 <has_text text="k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Corynebacteriales|f__Corynebacteriaceae|g__Corynebacterium|s__Corynebacterium_accolens"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
527 <has_text text="relative_abundance"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
528 <has_text text="NCBI_tax_id"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
529 <has_text text="clade_name"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
530 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
531 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
532 <output name="biom_output_file" ftype="biom1" file="SRS014464-Anterior_nares.biom" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
533 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
534 <has_text text="k__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Pseudomonadales|f__Moraxellaceae|g__Moraxella|s__Moraxella_lacunata"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
535 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
536 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
537 </test>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
538 <test expect_num_outputs="2">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
539 <section name="inputs">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
540 <conditional name="in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
541 <!-- bowtie2out -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
542 <param name="selector" value="bowtie2out"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
543 <param name="in" value="SRS014464-Anterior_nares-bowtie2out.tabular"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
544 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
545 <conditional name="db">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
546 <!-- Cached db -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
547 <param name="db_selector" value="cached"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
548 <param name="cached_db" value="test-db-20210409"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
549 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
550 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
551 <conditional name="in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
552 <param name="selector" value="bowtie2out"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
553 <param name="in" value="SRS014464-Anterior_nares-bowtie2out.tabular"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
554 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
555 <section name="mapping">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
556 <param name="bt2_ps" value="sensite"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
557 <param name="min_mapq_val" value="5"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
558 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
559 <section name="analysis">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
560 <param name="min_cu_len" value="2000"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
561 <param name="organism_profiling" value="add_viruses"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
562 <param name="stat" value="avg_g"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
563 <param name="stat_q" value="0.2"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
564 <param name="perc_nonzero" value="0.33"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
565 <param name="avoid_disqm" value="true"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
566 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
567 <section name="out">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
568 <param name="sample_id_key" value="SampleID"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
569 <param name="sample_id" value="Metaphlan_Analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
570 <param name="use_group_representative" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
571 <param name="legacy_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
572 <param name="CAMI_format_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
573 <param name="unknown_estimation" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
574 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
575 <output name="output_file" ftype="tabular" file="SRS014464-Anterior_nares-abundances.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
576 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
577 <has_text text="k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Corynebacteriales|f__Corynebacteriaceae|g__Corynebacterium|s__Corynebacterium_accolens"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
578 <has_text text="relative_abundance"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
579 <has_text text="NCBI_tax_id"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
580 <has_text text="clade_name"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
581 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
582 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
583 <output name="biom_output_file" ftype="biom1" file="SRS014464-Anterior_nares.biom" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
584 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
585 <has_text text="k__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Pseudomonadales|f__Moraxellaceae|g__Moraxella|s__Moraxella_lacunata"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
586 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
587 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
588 </test>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
589 <test expect_num_outputs="4">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
590 <section name="inputs">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
591 <conditional name="in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
592 <param name="selector" value="raw"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
593 <conditional name="raw_in">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
594 <!-- Single FASTA file -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
595 <param name="selector" value="single"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
596 <param name="in" value="SRS014464-Anterior_nares.fasta"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
597 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
598 <param name="read_min_len" value="70"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
599 <section name="mapping">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
600 <param name="bt2_ps" value="sensitive"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
601 <param name="min_mapq_val" value="5"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
602 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
603 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
604 <conditional name="db">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
605 <!-- Cached db -->
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
606 <param name="db_selector" value="cached"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
607 <param name="cached_db" value="test-db-20210409"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
608 </conditional>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
609 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
610 <section name="analysis">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
611 <param name="min_cu_len" value="2000"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
612 <param name="organism_profiling" value="add_viruses"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
613 <param name="stat" value="avg_g"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
614 <param name="stat_q" value="0.2"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
615 <param name="perc_nonzero" value="0.33"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
616 <param name="ignore_markers" value="marker.txt"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
617 <param name="avoid_disqm" value="true"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
618 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
619 <section name="out">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
620 <param name="sample_id_key" value="SampleID"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
621 <param name="sample_id" value="Metaphlan_Analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
622 <param name="use_group_representative" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
623 <param name="legacy_output" value="true"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
624 <param name="CAMI_format_output" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
625 <param name="unknown_estimation" value="false"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
626 </section>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
627 <output name="output_file" ftype="tabular" file="SRS014464-Anterior_nares-legacy-abundances.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
628 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
629 <has_text text="k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Corynebacteriales|f__Corynebacteriaceae|g__Corynebacterium|s__Corynebacterium_accolens"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
630 <has_text text="SampleID"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
631 <has_text text="Metaphlan_Analysis"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
632 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
633 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
634 <output name="bowtie2out" ftype="tabular" file="SRS014464-Anterior_nares-bowtie2out.tabular" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
635 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
636 <has_text text="HWI-EAS109_102883399:3:104:7342:14360/1"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
637 <has_text text="37637__U2I1U8__N579_01580"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
638 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
639 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
640 <output name="sam_output_file" ftype="sam" file="SRS014464-Anterior_nares.sam" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
641 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
642 <has_text text="SN:13076__A0A2I1PE66__CYJ72_10760"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
643 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
644 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
645 <output name="biom_output_file" ftype="biom1" file="SRS014464-Anterior_nares.biom" compare="sim_size">
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
646 <assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
647 <has_text text="k__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Pseudomonadales|f__Moraxellaceae|g__Moraxella|s__Moraxella_lacunata"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
648 </assert_contents>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
649 </output>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
650 </test>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
651 </tests>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
652 <help><![CDATA[
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
653 What it does
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
654 ============
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
655
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
656 MetaPhlAn is a computational tool for profiling the composition of microbial communities (Bacteria,
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
657 Archaea and Eukaryotes) from metagenomic shotgun sequencing data (i.e. not 16S) with species-level.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
658
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
659 MetaPhlAn relies on ~1.1M unique clade-specific marker genes identified from ~100,000 reference genomes
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
660 (~99,500 bacterial and archaeal and ~500 eukaryotic), allowing:
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
661
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
662 - unambiguous taxonomic assignments;
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
663 - accurate estimation of organismal relative abundance;
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
664 - species-level resolution for bacteria, archaea, eukaryotes and viruses;
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
665 - strain identification and tracking
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
666 - orders of magnitude speedups compared to existing methods.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
667 - metagenomic strain-level population genomics
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
668
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
669 MetaPhlAn clade-abundance estimation
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
670 ------------------------------------
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
671
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
672 The basic usage of MetaPhlAn consists in the identification of the clades (from phyla to species and
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
673 strains in particular cases) present in the metagenome obtained from a microbiome sample and their
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
674 relative abundance.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
675
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
676 Marker level analysis
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
677 ---------------------
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
678
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
679 MetaPhlAn introduces the capability of characterizing organisms at the strain level using non
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
680 aggregated marker information. Such capability comes with several slightly different flavours and
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
681 are a way to perform strain tracking and comparison across multiple samples.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
682
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
683 Usually, MetaPhlAn is first ran with the default parameter for the type of analysis to profile the
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
684 species present in the community, and then a strain-level profiling can be performed to zoom-in into
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
685 specific species of interest. This operation can be performed quickly as it exploits the bowtie2out
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
686 intermediate file saved during the execution of the default analysis type.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
687
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
688 Inputs
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
689 ======
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
690
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
691 Metaphlan takes as input either:
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
692
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
693 - one or several sequence files in Fasta, FastQ (compressed or not)
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
694 - a BowTie2 produced SAM file
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
695 - an intermediary mapping file of the metagenome generated by a previous MetaPhlAn run
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
696
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
697 It also need the reference database, which can be locally installed or customized using the dedicated tools.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
698
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
699 Outputs
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
700 =======
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
701
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
702 The main output file is a tab-separated file with the predicted taxon relative abundances.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
703
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
704 It also generates a BIOM file and some intermediate files (SAM and BowTie2out) if sequence files are given as inputs.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
705
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
706
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
707 More help and use cases
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
708 =======================
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
709
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
710 To get more information about MetaPhlAn usage and use cases, please refer to the `Metaphlan documentation`_.
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
711
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
712 .. _Metaphlan documentation: https://github.com/biobakery/MetaPhlAn/wiki/MetaPhlAn-3.0#basic-usage
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
713
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
714 ]]></help>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
715 <expand macro="citations"/>
f5df500fcc3c "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit 1e543a44ceffd8e4c5537b9015606ab3b90a114c"
iuc
parents:
diff changeset
716 </tool>