Mercurial > repos > iuc > microsatbed
annotate find_str.py @ 2:3f6e5c701945 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 0c8ab2d9c2783fdc33d3eac057f2a29e298d7264
author | iuc |
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date | Wed, 14 Aug 2024 08:49:40 +0000 |
parents | 2b970db61912 |
children | 5f8efb080f49 |
rev | line source |
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0
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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changeset
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1 import argparse |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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2 import subprocess |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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3 |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
diff
changeset
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4 import pytrf # 1.3.0 |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
diff
changeset
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5 from pyfastx import Fastx # 0.5.2 |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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6 |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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7 """ |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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8 Allows all STR or those for a subset of motifs to be written to a bed file |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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9 Designed to build some of the microsatellite tracks from https://github.com/arangrhie/T2T-Polish/tree/master/pattern for the VGP. |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
diff
changeset
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10 """ |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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diff
changeset
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11 |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
diff
changeset
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12 |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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13 def getDensity(name, bed, chrlen, winwidth): |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
diff
changeset
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14 """ |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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15 pybigtools can write bigwigs and they are processed by other ucsc tools - but jb2 will not read them. |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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16 Swapped the conversion to use a bedgraph file processed by bedGraphToBigWig |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
diff
changeset
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17 """ |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
diff
changeset
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18 nwin = int(chrlen / winwidth) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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19 d = [0.0 for x in range(nwin + 1)] |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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20 for b in bed: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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21 nt = b[5] |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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22 bin = int(b[1] / winwidth) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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23 d[bin] += nt |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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24 bedg = [ |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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25 (name, (x * winwidth), ((x + 1) * winwidth) - 1, float(d[x])) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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26 for x in range(nwin + 1) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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27 if (x + 1) * winwidth <= chrlen |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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28 ] |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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29 return bedg |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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30 |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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31 |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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32 def write_ssrs(args): |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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33 """ |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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34 The integers in the call change the minimum repeats for mono-, di-, tri-, tetra-, penta-, hexa-nucleotide repeats |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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35 ssrs = pytrf.STRFinder(name, seq, 10, 6, 4, 3, 3, 3) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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36 NOTE: Dinucleotides GA and AG are reported separately by https://github.com/marbl/seqrequester. |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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37 The reversed pair STRs are about as common in the documentation sample. |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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38 Sequence read bias might be influenced by GC density or some other specific motif. |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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39 """ |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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changeset
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40 bed = [] |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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41 wig = [] |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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42 chrlens = {} |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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43 specific = None |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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44 if args.specific: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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45 specific = args.specific.upper().split(",") |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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46 fa = Fastx(args.fasta, uppercase=True) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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47 for name, seq in fa: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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48 chrlen = len(seq) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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49 chrlens[name] = chrlen |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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50 cbed = [] |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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51 for ssr in pytrf.STRFinder( |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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52 name, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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53 seq, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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54 args.monomin, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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55 args.dimin, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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56 args.trimin, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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57 args.tetramin, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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58 args.pentamin, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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59 args.hexamin, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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60 ): |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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61 row = ( |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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62 ssr.chrom, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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63 ssr.start, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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64 ssr.end, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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65 ssr.motif, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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66 ssr.repeat, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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67 ssr.length, |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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68 ) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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69 if args.specific and ssr.motif in specific: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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70 cbed.append(row) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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71 elif args.mono and len(ssr.motif) == 1: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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72 cbed.append(row) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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73 elif args.di and len(ssr.motif) == 2: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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74 cbed.append(row) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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75 elif args.tri and len(ssr.motif) == 3: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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76 cbed.append(row) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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77 elif args.tetra and len(ssr.motif) == 4: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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78 cbed.append(row) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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79 elif args.penta and len(ssr.motif) == 5: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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80 cbed.append(row) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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81 elif args.hexa and len(ssr.motif) == 6: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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82 cbed.append(row) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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83 if args.bigwig: |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
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84 w = getDensity(name, cbed, chrlen, args.winwidth) |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
iuc
parents:
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85 wig += w |
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86 bed += cbed |
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87 if args.bigwig: |
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88 wig.sort() |
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89 bedg = ["%s %d %d %.2f" % x for x in wig] |
2b970db61912
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
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90 with open("temp.bedg", "w") as bw: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
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91 bw.write("\n".join(bedg)) |
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92 chroms = ["%s\t%s" % (x, chrlens[x]) for x in chrlens.keys()] |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/microsatbed commit 275acb787c01484c6e435c8864090d377c3fde75
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93 with open("temp.chromlen", "w") as cl: |
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94 cl.write("\n".join(chroms)) |
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95 cmd = ["bedGraphToBigWig", "temp.bedg", "temp.chromlen", args.bed] |
2b970db61912
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96 subprocess.run(cmd) |
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97 else: |
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98 bed.sort() |
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99 obed = ["%s\t%d\t%d\t%s_%d\t%d" % x for x in bed] |
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100 with open(args.bed, "w") as outbed: |
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101 outbed.write("\n".join(obed)) |
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102 outbed.write("\n") |
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103 |
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104 |
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105 if __name__ == "__main__": |
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106 parser = argparse.ArgumentParser() |
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107 a = parser.add_argument |
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108 a("--di", action="store_true") |
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109 a("--tri", action="store_true") |
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110 a("--tetra", action="store_true") |
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111 a("--penta", action="store_true") |
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112 a("--hexa", action="store_true") |
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113 a("--mono", action="store_true") |
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114 a("--dimin", default=2, type=int) |
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115 a("--trimin", default=2, type=int) |
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116 a("--tetramin", default=2, type=int) |
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117 a("--pentamin", default=2, type=int) |
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118 a("--hexamin", default=2, type=int) |
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119 a("--monomin", default=2, type=int) |
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120 a("-f", "--fasta", default="humsamp.fa") |
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121 a("-b", "--bed", default="humsamp.bed") |
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122 a("--bigwig", action="store_true") |
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123 a("--winwidth", default=128, type=int) |
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124 a("--specific", default=None) |
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125 a("--minreps", default=2, type=int) |
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126 args = parser.parse_args() |
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127 write_ssrs(args) |