Mercurial > repos > iuc > modify_loom
view modify_loom.py @ 9:0f1aaff9b22d draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/anndata/ commit 67b3808b56df343798263ff0c905df8cb789edfa
author | iuc |
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date | Sat, 14 Sep 2024 19:59:12 +0000 |
parents | c8e4d0b9ae8c |
children |
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#!/usr/bin/env python """This program adds layers, row attributes or column attributes for loom files""" import argparse import loompy import numpy as np parser = argparse.ArgumentParser(description="Loompy file converter flags") parser.add_argument('--VERSION', action='version', version='%(prog)s 0.1.0', help="Displays tool version") parser.add_argument('--file', '-f', help="Loom file to which data will be added") parser.add_argument('--rowfile', '-r', help="File of row attributes & values") parser.add_argument('--colfile', '-c', help="File of column attributes and values") parser.add_argument('--layers', '-l', nargs='*', help="Input tsv files. First file becomes main layer.") parser.add_argument('--add', '-a', choices=["rows", "cols", "layers"], help="Selects rows, columns or layers to be added to file") args = parser.parse_args() lfile = args.file if args.rowfile: rowfile = args.rowfile if args.colfile: colfile = args.colfile if args.layers: alllayers = args.layers addselect = args.add # Check proper flags for chosen attributes are being added if addselect == "cols" and not args.colfile: raise Exception("To add column attributes, column flag and file must be provided") if addselect == "rows" and not args.rowfile: raise Exception("To add row attributes, row flag and file must be provided") if addselect == "layers" and not args.layers: raise Exception("To add layers, a layer flag and file(s) must be provided") layernames = [] rowdict = {} coldict = {} with loompy.connect(lfile) as loomfile: # Loom file dimensions nrow = loomfile.shape[0] ncol = loomfile.shape[1] if addselect == "layers": layernames = [] # Generate layer names based on file names for x in range(0, len(alllayers)): layer = alllayers[x] layer = layer.split("/")[-1].split(".")[-2] # Takes away path, takes off extension layernames.append(layer) # Add in the layers themselves for layer in range(0, len(alllayers)): matrix = "" with open(alllayers[layer], "r") as infile: rows = 0 count = 0 for line in infile: if count == 0: cols = len(line.split("\t")) if cols != ncol: raise Exception("Dimensions of new matrix incorrect for this loom file. New matrices must be %d by %d" % (nrow, ncol)) matrix = matrix + line + "\t" rows += 1 if rows != nrow: raise Exception("Dimensions of new matrix incorrect for this loom file. New matrices must be %d by %d") matrix = matrix.split("\t") matrix = [float(n) for n in matrix[:-1]] matrix = np.asarray(matrix).reshape(nrow, ncol) loomfile[layernames[layer]] = matrix elif addselect == "rows": with open(rowfile, "r") as rows: count = 0 for line in rows: line = line.strip().split("\t") if count == 0: # First time through row_attributes = line for x in row_attributes: rowdict[x] = [] count += 1 else: for x in range(0, len(line)): rowdict[row_attributes[x]].append(line[x]) for x in row_attributes: if len(rowdict[x]) != nrow: raise Exception("Incorrect length of row. Row length must be: %d" % nrow) loomfile.ra[x] = rowdict[x] elif addselect == "cols": with open(colfile, "r") as cols: count = 0 for line in cols: line = line.replace('\"', "") line = line.replace(' ', "") line = line.strip().split("\t") if count == 0: # First time through col_attributes = line for x in col_attributes: coldict[x] = [] count += 1 else: for x in range(0, len(line)): coldict[col_attributes[x]].append(line[x]) for y in col_attributes: if len(coldict[y]) != ncol: raise Exception("Incorrect length of column. Column length must be: %d" % ncol) loomfile.ca[y] = coldict[y]