Mercurial > repos > iuc > mothur_consensus_seqs
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 7f7605c2c8d8e92f3369dfdc290e5d8d06fa409a
author | iuc |
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date | Fri, 03 Aug 2018 14:26:07 -0400 |
parents | 4f4de4518aca |
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<tool profile="16.07" id="mothur_consensus_seqs" name="Consensus.seqs" version="@WRAPPER_VERSION@.0"> <description>Find a consensus sequence for each OTU or phylotype</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"/> <expand macro="stdio"/> <expand macro="version_command"/> <command><![CDATA[ @SHELL_OPTIONS@ ## create symlinks to input datasets ln -s '$fasta' fasta.dat && ln -s '$name' name.dat && ln -s '$count' count.dat && #if $perotu.use == "yes": ln -s '$perotu.otu' perotu.otu.dat && #end if echo 'consensus.seqs( fasta=fasta.dat #if $name: ,name=name.dat #end if #if $cutoff: ,cutoff=$cutoff #end if #if $perotu.use == "yes": ,list=perotu.otu.dat #if $perotu.label: ,label=${ str($perotu.label).replace(",","-") } #end if #end if #if $count: ,count=count.dat #end if )' | sed 's/ //g' ## mothur trips over whitespace | mothur | tee mothur.out.log ]]></command> <inputs> <param name="fasta" type="data" format="mothur.align" label="fasta - Sequences to Bin" help="Sequences must be aligned"/> <param name="name" type="data" format="mothur.names" optional="true" label="name - Sequences Name reference"/> <conditional name="perotu"> <param name="use" type="select" label="Consensus sequence"> <option value="no" selected="true">Single consensus sequence</option> <option value="yes">Consensus sequence for each OTU in list</option> </param> <when value="yes"> <param name="otu" type="data" format="mothur.list" label="list - OTU List"/> <param name="label" type="select" optional="true" multiple="true" label="label - Select OTU Labels" help="By default all labels are used"> <expand macro="labeloptions"/> </param> </when> <when value="no"/> </conditional> <param name="cutoff" type="integer" value="" optional="True" min="1" max="100" label="Cutoff - set a percentage of sequences that support the base" help="For example: cutoff=95 would return the base that was supported by at least 95% of sequences. Must be between 1 and 100. Optional"/> <param name="count" type="data" format="mothur.count_table" optional="true" label="Count - a count table" help="this file can be generated by count.seqs"/> <expand macro="param-savelog"/> </inputs> <outputs> <expand macro="logfile-output"/> <data name="summary" format="tabular" from_work_dir="fasta*.cons.summary" label="${tool.name} on ${on_string}: cons.summary"> <filter>perotu['use'] == 'no'</filter> </data> <data name="cons_fasta" format="mothur.align" from_work_dir="fasta*.cons.fasta" label="${tool.name} on ${on_string}: cons.fasta"> <filter>perotu['use'] == 'no'</filter> </data> <collection name="consensusfastas" type="list" label="${tool.name} on ${on_string}: consensus fasta files per OTU"> <discover_datasets pattern=".*?\.(?P<designation>.*)\.cons\.fasta" format="mothur.align"/> <filter>perotu['use'] == 'yes'</filter> </collection> <collection name="consensusnames" type="list" label="${tool.name} on ${on_string}: consensus names files per OTU"> <discover_datasets pattern=".*?\.(?P<designation>.*)\.cons\.names" format="mothur.names"/> <filter>perotu['use'] == 'yes'</filter> </collection> <collection name="consensussummaries" type="list" label="${tool.name} on ${on_string}: consensus summary files per OTU"> <discover_datasets pattern=".*?\.(?P<designation>.*)\.cons\.summary" format="tabular"/> <filter>perotu['use'] == 'yes'</filter> </collection> </outputs> <tests> <test><!-- test with default values --> <param name="fasta" value="amazon.align_head" ftype="mothur.align"/> <output name="summary" md5="61a0e170a8b881135421afd0cea8305a" ftype="tabular"/> <output name="cons_fasta" md5="82f3475b61f240eba09597a409364c04" ftype="mothur.align"/> <param name="savelog" value="true"/> <expand macro="logfile-test"/> </test> <test><!-- test with list file --> <param name="fasta" value="amazon.align_head" ftype="mothur.align"/> <param name="use" value="yes"/> <param name="otu" value="amazon.align_head.list" ftype="mothur.list"/> <param name="count" value="amazon.align_head.count_table" ftype="mothur.count_table"/> <param name="labels" value="unique,0.30,0.33"/> <output_collection name="consensusfastas" count="3"> <element name="0.30" md5="a5c8c17814f02124194dba2e37f566c7" ftype="mothur.align"/> </output_collection> <output_collection name="consensusnames" count="3"> <element name="0.30" md5="9de4f7e774e0c16eaf7cf6ffbba9d475" ftype="mothur.names"/> </output_collection> <output_collection name="consensussummaries" count="3"> <element name="0.30" md5="d595234c6018dfcc1465e59d35d59205" ftype="tabular"/> </output_collection> <param name="savelog" value="true"/> <expand macro="logfile-test"/> </test> </tests> <help><![CDATA[ @MOTHUR_OVERVIEW@ **Command Documentation** The consensus.seqs_ command can be used in 2 ways: create a consensus sequence from a fastafile, or with a listfile create a consensus sequence for each otu. Sequences must be aligned. .. _consensus.seqs: https://www.mothur.org/wiki/Consensus.seqs ]]></help> <expand macro="citations"/> </tool>