view consensus.seqs.xml @ 3:f573fcdeec56 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 283d5933cd945b7815b3da2ce45baa60d73e9746
author iuc
date Fri, 06 Apr 2018 04:02:03 -0400
parents 4f4de4518aca
children
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<tool profile="16.07" id="mothur_consensus_seqs" name="Consensus.seqs" version="@WRAPPER_VERSION@.0">
    <description>Find a consensus sequence for each OTU or phylotype</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements"/>
    <expand macro="stdio"/>
    <expand macro="version_command"/>
    <command><![CDATA[
@SHELL_OPTIONS@

## create symlinks to input datasets
ln -s '$fasta' fasta.dat &&
ln -s '$name' name.dat &&
ln -s '$count' count.dat &&
#if $perotu.use == "yes":
    ln -s '$perotu.otu' perotu.otu.dat &&
#end if

echo 'consensus.seqs(
    fasta=fasta.dat
    #if $name:
        ,name=name.dat
    #end if
    #if $cutoff:
        ,cutoff=$cutoff
    #end if
    #if $perotu.use == "yes":
        ,list=perotu.otu.dat
        #if $perotu.label:
            ,label=${ str($perotu.label).replace(",","-") }
        #end if
    #end if
    #if $count:
        ,count=count.dat
    #end if
)'
| sed 's/ //g'  ## mothur trips over whitespace
| mothur
| tee mothur.out.log
    ]]></command>
    <inputs>
        <param name="fasta" type="data" format="mothur.align" label="fasta - Sequences to Bin" help="Sequences must be aligned"/>
        <param name="name" type="data" format="mothur.names" optional="true" label="name - Sequences Name reference"/>
        <conditional name="perotu">
            <param name="use" type="select" label="Consensus sequence">
                <option value="no" selected="true">Single consensus sequence</option>
                <option value="yes">Consensus sequence for each OTU in list</option>
            </param>
            <when value="yes">
                <param name="otu" type="data" format="mothur.list" label="list - OTU List"/>
                <param name="label" type="select" optional="true" multiple="true" label="label - Select OTU Labels" help="By default all labels are used">
                    <expand macro="labeloptions"/>
                </param>
            </when>
            <when value="no"/>
        </conditional>
        <param name="cutoff" type="integer" value="" optional="True" min="1" max="100" label="Cutoff - set a percentage of sequences that support the base"
            help="For example: cutoff=95 would return the base that was supported by at least 95% of sequences. Must be between 1 and 100. Optional"/>
        <param name="count" type="data" format="mothur.count_table" optional="true" label="Count - a count table" help="this file can be generated by count.seqs"/>
        <expand macro="param-savelog"/>
    </inputs>
    <outputs>
        <expand macro="logfile-output"/>
        <data name="summary" format="tabular" from_work_dir="fasta*.cons.summary" label="${tool.name} on ${on_string}: cons.summary">
            <filter>perotu['use'] == 'no'</filter>
        </data>
        <data name="cons_fasta" format="mothur.align" from_work_dir="fasta*.cons.fasta" label="${tool.name} on ${on_string}: cons.fasta">
            <filter>perotu['use'] == 'no'</filter>
        </data>
        <collection name="consensusfastas" type="list" label="${tool.name} on ${on_string}: consensus fasta files per OTU">
            <discover_datasets pattern=".*?\.(?P&lt;designation&gt;.*)\.cons\.fasta" format="mothur.align"/>
            <filter>perotu['use'] == 'yes'</filter>
        </collection>
        <collection name="consensusnames" type="list" label="${tool.name} on ${on_string}: consensus names files per OTU">
            <discover_datasets pattern=".*?\.(?P&lt;designation&gt;.*)\.cons\.names" format="mothur.names"/>
            <filter>perotu['use'] == 'yes'</filter>
        </collection>
        <collection name="consensussummaries" type="list" label="${tool.name} on ${on_string}: consensus summary files per OTU">
            <discover_datasets pattern=".*?\.(?P&lt;designation&gt;.*)\.cons\.summary" format="tabular"/>
            <filter>perotu['use'] == 'yes'</filter>
        </collection>
    </outputs>
    <tests>
        <test><!-- test with default values -->
            <param name="fasta" value="amazon.align_head" ftype="mothur.align"/>
            <output name="summary" md5="61a0e170a8b881135421afd0cea8305a" ftype="tabular"/>
            <output name="cons_fasta" md5="82f3475b61f240eba09597a409364c04" ftype="mothur.align"/>
            <param name="savelog" value="true"/>
            <expand macro="logfile-test"/>
        </test>
        <test><!-- test with list file -->
            <param name="fasta" value="amazon.align_head" ftype="mothur.align"/>
            <param name="use" value="yes"/>
            <param name="otu" value="amazon.align_head.list" ftype="mothur.list"/>
            <param name="count" value="amazon.align_head.count_table" ftype="mothur.count_table"/>
            <param name="labels" value="unique,0.30,0.33"/>
            <output_collection name="consensusfastas" count="3">
                <element name="0.30" md5="a5c8c17814f02124194dba2e37f566c7" ftype="mothur.align"/>
            </output_collection>
            <output_collection name="consensusnames" count="3">
                <element name="0.30" md5="9de4f7e774e0c16eaf7cf6ffbba9d475" ftype="mothur.names"/>
            </output_collection>
            <output_collection name="consensussummaries" count="3">
                <element name="0.30" md5="d595234c6018dfcc1465e59d35d59205" ftype="tabular"/>
            </output_collection>
            <param name="savelog" value="true"/>
            <expand macro="logfile-test"/>
        </test>
    </tests>
    <help><![CDATA[

@MOTHUR_OVERVIEW@

**Command Documentation**

The consensus.seqs_ command can be used in 2 ways: create a consensus sequence from a fastafile, or with a listfile create a consensus sequence for each otu. Sequences must be aligned.

.. _consensus.seqs: https://www.mothur.org/wiki/Consensus.seqs

    ]]></help>
    <expand macro="citations"/>
</tool>