Mercurial > repos > iuc > mothur_fastq_info
diff fastq.info.xml @ 0:19316e7c4802 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
author | iuc |
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date | Fri, 19 May 2017 05:29:45 -0400 |
parents | |
children | 1aa945e00ef2 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastq.info.xml Fri May 19 05:29:45 2017 -0400 @@ -0,0 +1,140 @@ +<tool profile="16.07" id="mothur_fastq_info" name="Fastq.info" version="@WRAPPER_VERSION@.0"> + <description>Convert fastq to fasta and quality</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <expand macro="stdio"/> + <expand macro="version_command"/> + <command><![CDATA[ + @SHELL_OPTIONS@ + + ## create symlinks to input datasets + ln -s "$fastq" fastq.dat && + #if $oligo.add == "yes": + ln -s "$oligo.oligos" oligo.oligos.dat && + #end if + + echo 'fastq.info( + fastq=fastq.dat, + pacbio=$pacbio, + format=$format + #if $oligo.add == "yes": + ,oligos=oligo.oligos.dat + ,bdiffs=$oligo.bdiffs + ,pdiffs=$oligo.pdiffs + ,tdiffs=$oligo.tdiffs + ,ldiffs=$oligo.ldiffs + ,sdiffs=$oligo.sdiffs + #end if + )' + | sed 's/ //g' ## mothur trips over whitespace + | mothur + | tee mothur.out.log + ## rename some outputs + #if $oligo.add == "yes": + && mv fastq.scrap.fasta scrap.fasta + && mv fastq.scrap.qual scrap.qual + #end if + ]]></command> + <inputs> + <param name="fastq" type="data" format="fastq" label="fastq - Fastq Sequence file"/> + <param name="pacbio" type="boolean" truevalue="true" falsevalue="false" checked="false" label="pacbio - if set to true, quality scores of 0 will results in a corresponding base of N"/> + <param name="format" type="select" label="format of sequence"> + <option value="sanger" selected="true">sanger</option> + <option value="solexa">solexa</option> + <option value="illumina">illumina</option> + <option value="illumina1.8+">illumina1.8+</option> + </param> + <conditional name="oligo"> + <param name="add" type="select" label="Use oligos file?" help="a file that contain the sequences of the forward and reverse primers and barcodes and their sample identifier. Each line of the oligos file can start with the key words "forward", "reverse", and "barcode" or it can start with a "#" to tell mothur to ignore that line of the oligos file."> + <option value="no">no</option> + <option value="yes">yes</option> + </param> + <when value="yes"> + <param name="oligos" type="data" format="mothur.oligos" label="oligos - barcodes and primers"/> + <param name="bdiffs" type="integer" value="0" min="0" label="bdiffs - number of differences to allow in the barcode (must be > 0; default 0)"/> + <param name="pdiffs" type="integer" value="0" min="0" label="pdiffs - number of differences to allow in the primer (must be > 0; default 0)"/> + <param name="tdiffs" type="integer" value="0" min="0" label="tdiffs - total number of differences to allow in primer and barcode (must be > 0; default 0)"/> + <param name="ldiffs" type="integer" value="0" min="0" label="ldiffs - total number of differences to allow in linker sequence (must be > 0; default 0)"/> + <param name="sdiffs" type="integer" value="0" min="0" label="sdiffs - total number of differences to allow in spacer sequence (must be > 0; default 0)"/> + </when> + <when value="no"/> + </conditional> + </inputs> + <outputs> + <expand macro="logfile-output"/> + <data name="fasta_out" format="fasta" from_work_dir="fastq*.fasta" label="${tool.name} on ${on_string}: fasta"/> + <data name="qfile_out" format="qual454" from_work_dir="fastq*.qual" label="${tool.name} on ${on_string}: qual"/> + <data name="fastq_scrap_out" format="fastq" from_work_dir="fastq*.scrap.fastq" label="${tool.name} on ${on_string}: fastq scrap"> + <filter>oligos</filter> + </data> + <data name="fasta_scrap_out" format="fasta" from_work_dir="scrap.fasta" label="${tool.name} on ${on_string}: fasta scrap"> + <filter>oligos</filter> + </data> + <data name="qfile_scrap_out" format="qual454" from_work_dir="scrap.qual" label="${tool.name} on ${on_string}: qual scrap"> + <filter>oligos</filter> + </data> + </outputs> + <tests> + <test> + <param name="fastq" value="Mock_S280_L001_R1_001_small.fastq"/> + <param name="pacbio" value="false"/> + <param name="format" value="sanger"/> + <param name="add" value="no"/> + <output name="fasta_out" md5="88c573d00181be6dd519a0c9599f8b0c"/> + <output name="qfile_out" md5="d382c496c583b1501666459757cabbe3"/> + <expand macro="logfile-test"/> + </test> + <test> + <param name="fastq" value="Mock_S280_L001_R1_001_small.fastq"/> + <param name="pacbio" value="false"/> + <param name="format" value="sanger"/> + <param name="add" value="yes"/> + <param name="oligos" value="GQY1XT001.oligos"/> + <param name="bdiffs" value="1"/> + <param name="pdiffs" value="3"/> + <param name="tdiffs" value="3"/> + <param name="ldiffs" value="3"/> + <param name="sdiffs" value="7"/> + <output name="fasta_out" md5="88c573d00181be6dd519a0c9599f8b0c" ftype="fasta"/> + <output name="qfile_out" md5="d382c496c583b1501666459757cabbe3" ftype="qual454"/> + <output name="fastq_scrap_out" md5="37a280b8af54af2a0a52b2eba1e58933" ftype="fastq"/> + <output name="fasta_scrap_out" md5="88c573d00181be6dd519a0c9599f8b0c" ftype="fasta"/> + <output name="qfile_scrap_out" md5="96c2f7ea3cf6336d50bef2c087280c01" ftype="qual454"/> + <expand macro="logfile-test"/> + </test> + <test> + <param name="fastq" value="Mock_S280_L001_R1_001_small.fastq"/> + <param name="pacbio" value="false"/> + <param name="format" value="sanger"/> + <param name="add" value="yes"/> + <param name="oligos" value="HMPv5-v3_1368-1369.oligos"/> + <param name="bdiffs" value="1"/> + <param name="pdiffs" value="3"/> + <param name="tdiffs" value="3"/> + <param name="ldiffs" value="3"/> + <param name="sdiffs" value="7"/> + <output name="fasta_out" md5="88c573d00181be6dd519a0c9599f8b0c"/> + <output name="qfile_out" md5="d382c496c583b1501666459757cabbe3"/> + <output name="fastq_scrap_out" md5="37a280b8af54af2a0a52b2eba1e58933"/> + <output name="fasta_scrap_out" md5="88c573d00181be6dd519a0c9599f8b0c"/> + <output name="qfile_scrap_out" md5="96c2f7ea3cf6336d50bef2c087280c01"/> + <expand macro="logfile-test"/> + </test> + </tests> + <help> +<![CDATA[ + +@MOTHUR_OVERVIEW@ + +**Command Documentation** + +The fastq.info_ command reads a fastq file and creates a fasta and quality file. + + +.. _fastq.info: https://www.mothur.org/wiki/Fastq.info +]]> + </help> + <expand macro="citations"/> +</tool>