diff filter.shared.xml @ 0:bccb5efbc4ee draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
author iuc
date Fri, 19 May 2017 05:47:34 -0400
parents
children 644b7baba854
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/filter.shared.xml	Fri May 19 05:47:34 2017 -0400
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+<tool profile="16.07" id="mothur_filter_shared" name="Filter.shared" version="@WRAPPER_VERSION@.0">
+    <description>remove OTUs based on various critieria</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
+    <command><![CDATA[
+        @SHELL_OPTIONS@
+
+        ## create symlinks to input datasets
+        ln -s "$otu" otu.dat &&
+
+        echo 'filter.shared(
+            shared=otu.dat,
+            #if $label:
+                label=${ str($label).replace(",","-") },
+            #end if
+            #if $label:
+                groups=${ str($groups).replace(",","-") },
+            #end if
+            minabund=$minabund,
+            minpercent=$minpercent,
+            rarepercent=$rarepercent,
+            keepties=$keepties,
+            minnumsamples=$minnumsamples,
+            minpercentsamples=$minpercentsamples,
+            mintotal=$mintotal,
+            makerare=$makerare
+        )'
+        | sed 's/ //g'  ## mothur trips over whitespace
+        | mothur
+        | tee mothur.out.log
+    ]]></command>
+    <inputs>
+        <param name="otu" type="data" format="mothur.shared" label="shared - specify your shared file"/>
+        <param name="label" type="select" optional="true" multiple="true" label="label - OTU Labels" help="If none selected, all labels will be output">
+            <expand macro="labeloptions"/>
+        </param>
+        <param name="groups" type="select" optional="true" multiple="true" label="Select groups for to include in this grouping">
+            <options>
+                <filter type="data_meta" ref="otu" key="groups"/>
+            </options>
+        </param>
+        <param name="minabund" type="integer" value="0" min="0" label="minabund - indicate the minimum abundance required for each sample in a given OTU" help="If any samples abundance falls below the minimum, the OTU is removed. Default=0"/>
+        <param name="minpercent" type="integer" value="0" min="0" max="100" label="minpercent - indicate the minimum relative abundance of an OTU" help="For example, if the OTUs total abundance across all samples is 8, and the total abundance across all OTUs is 1000, and minpercent=1. The OTU's relative abundance is 0.008, the minimum is 0.01, so the OTU will be removed (Default=0)"/>
+        <param name="rarepercent" type="integer" value="0" min="0" max="100" label="rarepercent - indicate the percentage of otus to remove" help="The OTUs chosen to be removed are the rarest.  For example if you have 1000 OTUs, rarepercent=20 would remove the 200 OTUs with the lowest abundance (Default=0)"/>
+        <param name="keepties" type="boolean" truevalue="true" falsevalue="false" checked="true" label="keepties - indicate you want to keep the OTUs with the same abundance as the first 'not rare' OTU" help="For example if you have 10 OTUs, rarepercent=20 abundances of 20, 18, 15, 15, 10, 5, 3, 3, 3, 1. keepties=t, would remove the 10th OTU, but keep the 9th because its abundance ties the 8th OTU. keepties=f would remove OTUs 9 and 10 (Default=T)"/>
+        <param name="minnumsamples" type="integer" value="0" min="0" label="minnumsamples - indicate the minimum number of samples present in an OTU" help="If the number of samples present falls below the minimum, the OTU is removed (Default=0)"/>
+        <param name="minpercentsamples" type="integer" value="0" min="0" max="100" label="minpercentsamples - indicate the minimum percent of sample present in an OTU" help="For example, if the total number of samples is 10, the number present is 3, and the minpercentsamples=50. The OTU's precent of samples is 0.333, the minimum is 0.50, so the OTU will be removed (Default=0)"/>
+        <param name="mintotal" type="integer" value="0" min="0" max="100" label="mintotal - indicate the minimum abundance required for a given OTU" help="If abundance across all samples falls below the minimum, the OTU is removed (Default=0)"/>
+        <param name="makerare" type="boolean" truevalue="true" falsevalue="false" checked="true" label="makerare - indicate you want the abundances of any removed OTUs to be saved and a new rare OTU created with its abundances equal to the sum of the OTUs removed" help="This will preserve the number of reads in your dataset (Default=T)"/>
+    </inputs>
+    <outputs>
+        <expand macro="logfile-output"/>
+        <collection name="filter_shared" type="list" label="${tool.name} on ${on_string}: filter.shared">
+            <discover_datasets pattern=".*?\.(?P&lt;designation&gt;.*)\.filter\.dat" format="mothur.shared"/>
+        </collection>
+    </outputs>
+    <tests>
+        <test><!-- test with defaults -->
+            <param name="otu" value="amazon.an.shared" ftype="mothur.shared"/>
+            <param name="minabund" value="1"/>
+            <output_collection name="filter_shared" count="36">
+                <element name="0.29" md5="db8387475c126110f2ac21066344c933" ftype="mothur.shared"/>
+            </output_collection>
+            <expand macro="logfile-test"/>
+        </test>
+        <test><!-- test with label and group select -->
+            <param name="otu" value="amazon.an.shared" ftype="mothur.shared"/>
+            <param name="label" value="0.05,0.29,0.33"/>
+            <param name="groups" value="forest,pasture"/>
+            <param name="minabund" value="1"/>
+            <output_collection name="filter_shared" count="3">
+                <element name="0.29" md5="db8387475c126110f2ac21066344c933" ftype="mothur.shared"/>
+            </output_collection>
+            <expand macro="logfile-test"/>
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+
+@MOTHUR_OVERVIEW@
+
+**Command Documentation**
+
+The filter.shared_ is used to remove OTUs based on various critieria.
+
+.. _filter.shared: https://www.mothur.org/wiki/Filter.shared
+
+]]>
+    </help>
+    <citations>
+        <citation type="doi">10.1128/AEM.01541-09</citation>
+    </citations>
+</tool>