Mercurial > repos > iuc > mothur_screen_seqs
comparison screen.seqs.xml @ 0:125a6ae65887 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
author | iuc |
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date | Fri, 19 May 2017 05:48:41 -0400 |
parents | |
children | 8743aecd26a9 |
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1 <tool profile="16.07" id="mothur_screen_seqs" name="Screen.seqs" version="@WRAPPER_VERSION@.0"> | |
2 <description>Screen sequences</description> | |
3 <macros> | |
4 <import>macros.xml</import> | |
5 </macros> | |
6 <expand macro="requirements"/> | |
7 <expand macro="stdio"/> | |
8 <expand macro="version_command"/> | |
9 <command><![CDATA[ | |
10 @SHELL_OPTIONS@ | |
11 | |
12 ## create symlinks to input datasets | |
13 ln -s "$fasta_in" fasta_in.dat && | |
14 ln -s "$names_in" names_in.dat && | |
15 ln -s "$groups_in" groups_in.dat && | |
16 ln -s "$qfile_in" qfile_in.dat && | |
17 ln -s "$count_in" count_in.dat && | |
18 ln -s "$alignreport_in" alignreport_in.dat && | |
19 ln -s "$taxonomy_in" taxonomy_in.dat && | |
20 ln -s "$summary" summary.dat && | |
21 ln -s "$contigsreport" contigsreport.dat && | |
22 | |
23 echo 'screen.seqs( | |
24 fasta=fasta_in.dat | |
25 #if int($start) > -1: | |
26 ,start=$start | |
27 #end if | |
28 #if int($end) > -1: | |
29 ,end=$end | |
30 #end if | |
31 #if int($minlength) > -1: | |
32 ,minlength=$minlength | |
33 #end if | |
34 #if int($maxlength) > -1: | |
35 ,maxlength=$maxlength | |
36 #end if | |
37 #if int($maxambig) > -1: | |
38 ,maxambig=$maxambig | |
39 #end if | |
40 #if int($maxhomop) > -1: | |
41 ,maxhomop=$maxhomop | |
42 #end if | |
43 #if int($criteria) > -1: | |
44 ,criteria=$criteria | |
45 #end if | |
46 #if $optimize: | |
47 ,optimize=$optimize | |
48 #end if | |
49 #if $qfile_in: | |
50 ,qfile=qfile_in.dat | |
51 #end if | |
52 #if $names_in: | |
53 ,name=names_in.dat | |
54 #end if | |
55 #if $groups_in: | |
56 ,group=groups_in.dat | |
57 #end if | |
58 #if $alignreport_in: | |
59 ,alignreport=alignreport_in.dat | |
60 #end if | |
61 #if $taxonomy_in: | |
62 ,taxonomy=taxonomy_in.dat | |
63 #end if | |
64 #if $count_in: | |
65 ,count=count_in.dat | |
66 #end if | |
67 #if $summary: | |
68 ,summary=summary.dat | |
69 #end if | |
70 #if $contigsreport: | |
71 ,contigsreport=contigsreport.dat | |
72 #end if | |
73 ,processors='"\${GALAXY_SLOTS:-8}"' | |
74 )' | |
75 | sed 's/ //g' ## mothur trips over whitespace | |
76 | mothur | |
77 | tee mothur.out.log | |
78 ]]></command> | |
79 <inputs> | |
80 <param name="fasta_in" type="data" format="fasta,mothur.align" label="fasta - Fasta to screen"/> | |
81 <param name="start" type="integer" value="-1" label="start - Remove sequences that start after position (ignored when negative)"/> | |
82 <param name="end" type="integer" value="-1" label="end - Remove sequences that end before position (ignored when negative)"/> | |
83 <param name="minlength" type="integer" value="-1" label="minlength - Remove sequences shorter than (ignored when negative)"/> | |
84 <param name="maxlength" type="integer" value="-1" label="maxlength - Remove sequences longer than (ignored when negative)"/> | |
85 <param name="maxambig" type="integer" value="-1" label="maxambig - Remove sequences with ambiguous bases greater than (ignored when negative)"/> | |
86 <param name="maxhomop" type="integer" value="-1" label="maxhomop - Remove sequences with homopolymers greater than (ignored when negative)"/> | |
87 <param name="criteria" type="integer" value="-1" label="criteria - Percent of sequences that an optimize value must match to be retained(ignored when negative)"/> | |
88 <param name="optimize" type="select" multiple="true" display="checkboxes" label="optimize - Optimize selected paramenters"> | |
89 <option value="start">start</option> | |
90 <option value="end">end</option> | |
91 <option value="minlength">minlength</option> | |
92 <option value="maxlength">maxlength</option> | |
93 <option value="maxambig">maxambig</option> | |
94 <option value="maxhomop">maxhomop</option> | |
95 </param> | |
96 <param name="qfile_in" type="data" format="qual" optional="true" label="qfile - Sequence Quality file to screen"/> | |
97 <param name="names_in" type="data" format="mothur.names" optional="true" label="name - Sequence Names to screen"/> | |
98 <param name="groups_in" type="data" format="mothur.groups" optional="true" label="group - Groups to screen"/> | |
99 <param name="alignreport_in" type="data" format="mothur.align.report" optional="true" label="alignreport - Align Report to screen"/> | |
100 <param name="summary" type="data" format="mothur.summary" optional="true" label="summary file - as created by summary.seqs" help="saves processing time when screening with parameters in the summary file"/> | |
101 <param name="contigsreport" type="data" format="contigs.report" optional="true" label="contigsreport - Contigs Report to screen with" help="this file is created by the make.contigs command. If you provide the contigs report file you can screen your sequences using the following parameters: minoverlap, ostart, oend and mismatches"/> | |
102 <param name="taxonomy_in" type="data" format="taxonomy" optional="true" label="taxonomy - Taxonomy to screen"/> | |
103 <param name="count_in" type="data" format="mothur.count_table" optional="true" label="count - a count_table" help="generated by count.seqs"/> | |
104 </inputs> | |
105 <outputs> | |
106 <expand macro="logfile-output"/> | |
107 <data name="fasta_out" format_source="fasta_in" from_work_dir="fasta_in*.good.dat" label="${tool.name} on ${on_string}: good.${fasta_in.datatype.file_ext}"/> | |
108 <data name="bad_accnos" format="mothur.accnos" from_work_dir="fasta_in*.bad.accnos" label="${tool.name} on ${on_string}: bad.accnos"/> | |
109 <data name="qfile_out" format_source="qfile_in" from_work_dir="qfile_in*.good.dat" label="${tool.name} on ${on_string}: qfile"> | |
110 <filter>qfile_in</filter> | |
111 </data> | |
112 <data name="names_out" format="mothur.names" from_work_dir="names_in*.good.dat" label="${tool.name} on ${on_string}: names"> | |
113 <filter>names_in</filter> | |
114 </data> | |
115 <data name="groups_out" format="mothur.groups" from_work_dir="groups_in*.good.dat" label="${tool.name} on ${on_string}: groups"> | |
116 <filter>groups_in</filter> | |
117 </data> | |
118 <data name="alignreport_out" format="mothur.align.report" from_work_dir="alignreport_in*.good.dat" label="${tool.name} on ${on_string}: align.report"> | |
119 <filter>alignreport_in</filter> | |
120 </data> | |
121 <data name="count_out" format="mothur.count_table" from_work_dir="count_in*.good.dat" label="${tool.name} on ${on_string}: count"> | |
122 <filter>count_in</filter> | |
123 </data> | |
124 </outputs> | |
125 <tests> | |
126 <test> | |
127 <param name="fasta_in" value="Mock_S280_L001_R1_001_small.trim.contigs.fasta" ftype="fasta"/> | |
128 <param name="maxambig" value="0"/> | |
129 <param name="maxlength" value="275"/> | |
130 <output name="fasta_out" file="Mock_S280_L001_R1_001_small.trim.contigs.good.fasta" ftype="fasta"/> | |
131 <output name="bad_accnos" file="Mock_S280_L001_R1_001_small.trim.contigs.bad.accnos" ftype="mothur.accnos"/> | |
132 <expand macro="logfile-test"/> | |
133 </test> | |
134 <test> | |
135 <param name="fasta_in" value="amazon.fasta" ftype="mothur.align"/> | |
136 <param name="count_in" value="amazon.count_table"/> | |
137 <param name="maxambig" value="0"/> | |
138 <param name="maxlength" value="275"/> | |
139 <output name="fasta_out" md5="d41d8cd98f00b204e9800998ecf8427e" ftype="mothur.align"/> | |
140 <output name="count_out" md5="5f4a08bbf3ec12f954edbcc6b2a2feee" ftype="mothur.count_table"/> | |
141 <output name="bad_accnos" md5="66acde5349e34fc97be22032ce68eea5" ftype="mothur.accnos"/> | |
142 <expand macro="logfile-test"/> | |
143 </test> | |
144 </tests> | |
145 <help> | |
146 <![CDATA[ | |
147 | |
148 @MOTHUR_OVERVIEW@ | |
149 | |
150 **Command Documentation** | |
151 | |
152 The screen.seqs_ command enables you to keep sequences that fulfill certain user defined criteria. Furthermore, it enables you to cull those sequences not meeting the criteria from a name_, group_, or align.report_ file. | |
153 | |
154 .. _name: https://www.mothur.org/wiki/Name_file | |
155 .. _group: https://www.mothur.org/wiki/Group_file | |
156 .. _align.report: https://www.mothur.org/wiki/Align.seqs | |
157 .. _screen.seqs: https://www.mothur.org/wiki/Screen.seqs | |
158 | |
159 ]]> | |
160 </help> | |
161 <expand macro="citations"/> | |
162 </tool> |