##FastQC	0.11.4
>>Basic Statistics	pass
#Measure	Value
Filename	poulet5_1
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267849
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.91988023102569	21.0	21.0	21.0	21.0	21.0
2	20.71684045861661	21.0	20.0	22.0	19.0	23.0
3	20.351574954545285	21.0	20.0	21.0	18.0	22.0
4	22.531542025544244	23.0	22.0	24.0	20.0	24.0
5	24.673566076408722	25.0	24.0	26.0	24.0	26.0
6	25.813099918237516	26.0	25.0	27.0	25.0	27.0
7	27.587864804423386	28.0	27.0	29.0	26.0	29.0
8	27.598941941168345	28.0	27.0	29.0	26.0	29.0
9	27.07115949658203	28.0	26.0	28.0	26.0	28.0
10-11	27.129873548155864	28.0	26.0	28.0	25.5	28.0
12-13	27.009298149330405	27.5	26.0	28.0	25.5	28.0
14-15	27.123565889736383	28.0	26.5	28.0	26.0	28.0
16-17	27.086309823818645	28.0	26.0	28.0	26.0	28.0
18-19	27.07924614241606	28.0	26.0	28.0	25.5	28.0
20-21	27.082520375286073	28.0	26.0	28.0	26.0	28.0
22-23	27.054301117420636	28.0	26.0	28.0	25.0	28.0
24-25	27.06350966402712	28.0	26.0	28.0	25.0	28.0
26-27	27.082996389756914	28.0	26.0	28.0	26.0	28.0
28-29	27.065495857740743	28.0	26.0	28.0	25.5	28.0
30-31	27.09608772106672	28.0	26.0	28.0	26.0	28.0
32-33	27.087097581099798	28.0	26.0	28.0	26.0	28.0
34-35	27.098251253504774	28.0	26.5	28.0	26.0	28.0
36-37	27.05184077595959	28.0	26.0	28.0	25.5	28.0
38-39	27.06521958267531	28.0	26.0	28.0	26.0	28.0
40-41	27.072148859992012	28.0	26.0	28.0	26.0	28.0
42-43	27.065725464720796	28.0	26.0	28.0	25.5	28.0
44-45	27.07168031241483	28.0	26.5	28.0	25.5	28.0
46-47	27.048083061725073	28.0	26.0	28.0	25.5	28.0
48-49	27.04642354460909	28.0	26.0	28.0	25.5	28.0
50-51	27.062865644448927	28.0	26.0	28.0	25.5	28.0
52-53	27.01859629866081	28.0	26.0	28.0	25.0	28.0
54-55	26.994157155710866	27.5	26.0	28.0	25.0	28.0
56-57	27.110127347871376	28.0	27.0	28.0	26.0	28.0
58-59	26.995846540401494	27.5	26.0	28.0	25.0	28.0
60-61	27.00461640700544	27.5	26.0	28.0	25.0	28.0
62-63	26.981614641085088	27.5	26.5	28.0	25.5	28.5
64-65	26.98900313236189	28.0	26.0	28.0	25.0	28.0
66-67	27.001189102815392	28.0	26.0	28.0	25.0	28.0
68-69	26.917739099268616	27.5	26.0	28.0	25.0	28.5
70-71	26.98481793846533	28.0	27.0	28.0	25.0	29.0
72-73	26.89518908041471	27.5	26.5	28.0	25.0	28.5
74-75	26.93677034448514	28.0	27.0	28.0	25.0	29.0
76-77	26.87640237596556	28.0	26.5	28.0	25.0	29.0
78-79	26.906094478605482	28.0	27.0	28.0	25.0	29.0
80-81	26.80463992772047	28.0	26.5	28.0	25.0	29.0
82-83	26.717012197170796	28.0	26.5	28.0	25.0	29.0
84-85	26.46960974280285	27.5	26.5	28.0	25.0	29.0
86-87	26.442842795754324	27.5	26.5	28.0	25.0	29.0
88-89	26.34282375517549	28.0	26.0	28.0	25.0	29.0
90-91	26.03493946215965	28.0	26.0	28.0	24.5	29.0
92-93	25.833215729758187	28.0	26.0	28.0	24.0	29.0
94-95	25.503102494315826	27.5	26.0	28.0	22.5	29.0
96-97	24.52807178671565	27.0	25.5	27.5	21.0	28.0
98-99	23.202399486277717	26.0	24.5	26.5	10.0	27.0
100-101	23.72906749698524	27.0	26.0	28.0	2.0	29.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.017259946109469126
1101	2	-0.13408255940416325
1101	3	-0.11853598095770224
1101	4	0.015421063929771606
1101	5	-0.06886052494072459
1101	6	-0.15241701542762698
1101	7	-0.029853727500473326
1101	8	0.04533865186875374
1101	9	0.07102084333114078
1101	10-11	0.02726717483210095
1101	12-13	0.05147474989913192
1101	14-15	-0.006227419450461014
1101	16-17	0.051879075943595865
1101	18-19	0.03525737425918152
1101	20-21	0.012963832365290529
1101	22-23	0.02506089814612622
1101	24-25	0.07843149833360386
1101	26-27	0.02737786083297422
1101	28-29	0.005161107012540356
1101	30-31	-0.08720467946375265
1101	32-33	-0.017197404032327057
1101	34-35	0.05763469947927646
1101	36-37	-0.045600552109540615
1101	38-39	-0.01952943529413176
1101	40-41	0.007678874836642535
1101	42-43	-0.008449851412301257
1101	44-45	0.039802961768131695
1101	46-47	0.023590251422636044
1101	48-49	0.033496458023201114
1101	50-51	-0.016386701090358002
1101	52-53	0.025786144269876132
1101	54-55	0.041314167697176885
1101	56-57	0.017577003151171056
1101	58-59	0.0409837724351334
1101	60-61	-0.01616144950176235
1101	62-63	-0.15311962828100434
1101	64-65	-0.1501215067991879
1101	66-67	-0.10208605868649911
1101	68-69	0.042677013693392496
1101	70-71	-0.05781314700952933
1101	72-73	0.069481248502548
1101	74-75	-0.0038247930645347594
1101	76-77	-0.14175773424430815
1101	78-79	-0.08483253524869738
1101	80-81	-0.13526874743146777
1101	82-83	-0.16462942511470757
1101	84-85	-0.008367476155605402
1101	86-87	0.07721301530740377
1101	88-89	0.039720943130355124
1101	90-91	0.0362011509996627
1101	92-93	0.09827090498435709
1101	94-95	0.07420306975118152
1101	96-97	0.1786375834862497
1101	98-99	0.29551833742201694
1101	100-101	0.3437055197576768
1102	1	0.017326703764346973
1102	2	0.13839251773801564
1102	3	0.026409722816328696
1102	4	0.035741352322840925
1102	5	0.0023402227450084467
1102	6	0.14763639069627388
1102	7	0.04905752932364038
1102	8	0.0695494280377531
1102	9	0.08835928980633767
1102	10-11	-0.0027737698628946816
1102	12-13	0.034666655904352695
1102	14-15	0.033050188548592985
1102	16-17	0.07202134392365522
1102	18-19	0.034278261987640946
1102	20-21	0.05398641128767423
1102	22-23	0.06921181495124884
1102	24-25	0.08067900604781997
1102	26-27	-0.017741041967884286
1102	28-29	0.06718267715258719
1102	30-31	0.061640783982721103
1102	32-33	0.007790357064276776
1102	34-35	0.07134783441875214
1102	36-37	0.05421141382922201
1102	38-39	0.071209052719162
1102	40-41	0.01045673503127631
1102	42-43	0.04671607844765191
1102	44-45	0.019738577718761974
1102	46-47	0.010641120755654043
1102	48-49	0.012718137942499652
1102	50-51	0.050487779683432876
1102	52-53	0.0492143723733669
1102	54-55	0.024116654048945918
1102	56-57	0.0937159629185551
1102	58-59	0.041755194224830205
1102	60-61	0.024132358354872707
1102	62-63	0.054253754700241075
1102	64-65	-0.0526961270223083
1102	66-67	0.03524185491426124
1102	68-69	-0.03408316314022031
1102	70-71	-0.002916102148386557
1102	72-73	0.06648086279165355
1102	74-75	-0.01905851903177691
1102	76-77	-0.07965271703567112
1102	78-79	-0.008930936031987358
1102	80-81	2.6766454123361427E-5
1102	82-83	0.029320106098204235
1102	84-85	0.003107422966163398
1102	86-87	-0.017180454310445015
1102	88-89	0.0031321851194370254
1102	90-91	-0.10882799984296554
1102	92-93	-0.031939900854709435
1102	94-95	0.11724506773361654
1102	96-97	0.03660696397521335
1102	98-99	-0.02817655042512257
1102	100-101	0.15842335740306268
1103	1	0.024960104530137528
1103	2	0.08766067725194304
1103	3	0.07296261762869705
1103	4	0.06324049483398753
1103	5	0.07631526106951014
1103	6	0.0966385536681571
1103	7	0.07774665636499023
1103	8	-0.009255547497563299
1103	9	0.026910588639452726
1103	10-11	0.028013579901557506
1103	12-13	-0.031132488706766992
1103	14-15	0.05921059846326315
1103	16-17	0.06106823631405334
1103	18-19	0.011463808575534529
1103	20-21	-0.028077021887696674
1103	22-23	0.053414770312830484
1103	24-25	0.10643692119492343
1103	26-27	0.03616525725022868
1103	28-29	0.013817120904818125
1103	30-31	0.03836083662204004
1103	32-33	0.03849545789796949
1103	34-35	0.09815573591603055
1103	36-37	0.07768385868743621
1103	38-39	-0.0028114850138507563
1103	40-41	-0.03808413393742427
1103	42-43	0.06455884649167132
1103	44-45	0.056353351726698975
1103	46-47	0.010648120664654925
1103	48-49	0.02254601018016089
1103	50-51	-0.0014795447413469276
1103	52-53	-0.0375250000147922
1103	54-55	-4.197769774521021E-4
1103	56-57	-0.01172891629801498
1103	58-59	8.302262494161994E-4
1103	60-61	0.028643799706134843
1103	62-63	-0.02307948996757858
1103	64-65	0.08427634233559189
1103	66-67	0.013911382210405776
1103	68-69	-0.07395639478820826
1103	70-71	0.007527762081377176
1103	72-73	0.02590414028904675
1103	74-75	-3.46012294365039E-4
1103	76-77	0.03972723102500453
1103	78-79	0.03319276635988189
1103	80-81	-0.02425241791647892
1103	82-83	-0.06252044997456707
1103	84-85	-0.1289423603866524
1103	86-87	-0.06115563263312751
1103	88-89	-0.24528583544629612
1103	90-91	-0.22759020593428758
1103	92-93	-0.19769774600272072
1103	94-95	-0.20408225501118693
1103	96-97	-0.15450280159783247
1103	98-99	-0.18504276116438234
1103	100-101	-0.0689879362501209
1104	1	0.020339101706095875
1104	2	0.03672574601341694
1104	3	0.11266781365937106
1104	4	0.006401989770132843
1104	5	-0.04066441196856374
1104	6	0.013070451362253266
1104	7	-0.01958566408358564
1104	8	-0.024315962893865617
1104	9	-0.02513147560151552
1104	10-11	0.022523903490988317
1104	12-13	0.059495715591634024
1104	14-15	-0.033870331255933195
1104	16-17	-0.040425200942163286
1104	18-19	-0.007150503758609261
1104	20-21	-0.016432537624311294
1104	22-23	-0.020815090937855274
1104	24-25	0.013523764707638719
1104	26-27	0.07530240888656792
1104	28-29	6.227081062348816E-5
1104	30-31	-0.053085794457398805
1104	32-33	0.03002503878851215
1104	34-35	0.038725687985689206
1104	36-37	-0.008191338467401721
1104	38-39	0.030650332523819657
1104	40-41	0.023318314018808906
1104	42-43	0.05951628148593002
1104	44-45	0.06560217050095218
1104	46-47	0.07727964295549938
1104	48-49	0.045884373871647455
1104	50-51	-0.014117042143841019
1104	52-53	0.043042771854491235
1104	54-55	-0.0066401611696349505
1104	56-57	0.08668924613372653
1104	58-59	-0.08276001871653449
1104	60-61	0.03825712073042098
1104	62-63	-0.002099319571293279
1104	64-65	0.0538915029039444
1104	66-67	0.08655463670451624
1104	68-69	0.12075508635135535
1104	70-71	0.08630793997210162
1104	72-73	0.06496719917830163
1104	74-75	0.14373372172118692
1104	76-77	0.10159211991994965
1104	78-79	0.21111925954164334
1104	80-81	0.21326423166059882
1104	82-83	0.16434387683747076
1104	84-85	0.13593410060308742
1104	86-87	0.1967595166935645
1104	88-89	0.31121456823143134
1104	90-91	0.2954304957495779
1104	92-93	0.33634233561852867
1104	94-95	0.39176310065103337
1104	96-97	0.4222121474229503
1104	98-99	0.2777933388659335
1104	100-101	0.4973131494215366
1105	1	-2.1743898997783617E-4
1105	2	-0.09137410901648124
1105	3	0.05778649909623468
1105	4	-0.14813488336767833
1105	5	-0.02899298364395264
1105	6	0.020622090089847944
1105	7	0.058124907739440346
1105	8	-0.015005206991791198
1105	9	-0.025104774715831724
1105	10-11	-0.03899729308564659
1105	12-13	-0.04749002142348857
1105	14-15	0.027507937050273767
1105	16-17	-0.03262304508123748
1105	18-19	0.07094958686733932
1105	20-21	0.00209709172027317
1105	22-23	0.017495968097801295
1105	24-25	-0.00441137727039731
1105	26-27	0.027858076306618074
1105	28-29	0.021098642128848155
1105	30-31	0.042807295196322315
1105	32-33	-0.002837468927257447
1105	34-35	-0.049198757931669945
1105	36-37	-0.015408430803773854
1105	38-39	0.048985987787112606
1105	40-41	-0.012837826478111936
1105	42-43	-0.03977096193033702
1105	44-45	-0.02278482899487244
1105	46-47	0.027747144051023298
1105	48-49	-0.08229165721446208
1105	50-51	0.04208282497998539
1105	52-53	0.01920359285960771
1105	54-55	0.006290920703129643
1105	56-57	-0.06481550583860596
1105	58-59	-0.05529108990895182
1105	60-61	-0.09674020132780825
1105	62-63	-0.08819396949912317
1105	64-65	-0.07267776312012231
1105	66-67	-0.04542941187224869
1105	68-69	0.027508525661030347
1105	70-71	-0.009777553122578553
1105	72-73	-0.052609365611399284
1105	74-75	-0.01063823418151344
1105	76-77	0.017351610906828796
1105	78-79	0.013272764504083767
1105	80-81	0.011573594404691079
1105	82-83	0.025337495325793213
1105	84-85	0.09654950889889591
1105	86-87	0.04994783804029268
1105	88-89	0.11074981575649545
1105	90-91	0.0597229301321498
1105	92-93	0.05132216074343532
1105	94-95	-0.22462527472572447
1105	96-97	-0.06926948472365879
1105	98-99	-0.11115218033278751
1105	100-101	-0.49488940663854564
1106	1	0.007770923227589321
1106	2	0.06527066673477577
1106	3	0.04134729053271258
1106	4	-0.008230631596923388
1106	5	-0.06395818521695773
1106	6	-0.022366510377121784
1106	7	0.003783022654133106
1106	8	0.03369156879309898
1106	9	0.021098791176946463
1106	10-11	-0.05754781176860391
1106	12-13	0.025255929037452773
1106	14-15	0.018321320500064076
1106	16-17	-0.020731597113510247
1106	18-19	0.04234354205963342
1106	20-21	-0.03856227050009764
1106	22-23	-0.026875880353156134
1106	24-25	0.08606523364591112
1106	26-27	0.01849277270681071
1106	28-29	-0.041204977299969414
1106	30-31	0.033667627792482335
1106	32-33	-2.0831673073828938E-4
1106	34-35	-0.0015460751032847497
1106	36-37	-0.016265427908344066
1106	38-39	-0.004899719771557898
1106	40-41	0.04291323373171707
1106	42-43	0.016214844011322782
1106	44-45	0.008372586587757525
1106	46-47	-0.020912492856894005
1106	48-49	0.028338049963007705
1106	50-51	0.0153995929727202
1106	52-53	0.008402486261221753
1106	54-55	-0.03896245052587233
1106	56-57	0.012921068807735026
1106	58-59	-0.00271855698148471
1106	60-61	-0.07464327026036699
1106	62-63	-0.007758169303478724
1106	64-65	-0.0026422499449303416
1106	66-67	-0.05082085563558181
1106	68-69	-0.025553119268884927
1106	70-71	-0.04405953950592689
1106	72-73	-0.08321600464113388
1106	74-75	0.0664683810232809
1106	76-77	0.11690832687710895
1106	78-79	0.02626086044291398
1106	80-81	-0.017148772168635418
1106	82-83	0.009564684347260766
1106	84-85	-0.05533588473115714
1106	86-87	-0.013130792670335012
1106	88-89	-0.0191409539011822
1106	90-91	4.240560707842178E-4
1106	92-93	-0.2420294196909687
1106	94-95	-0.08239318617543034
1106	96-97	0.05337990465714171
1106	98-99	0.1124455818046215
1106	100-101	0.05996891905679291
1107	1	0.0026459002454259917
1107	2	0.21875323093162535
1107	3	0.018365124693403345
1107	4	0.01182513845884614
1107	5	0.11897219414199256
1107	6	-0.019738882749493314
1107	7	0.02129543302368475
1107	8	0.017083123613225837
1107	9	0.021612059547358342
1107	10-11	0.04390312896805071
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2207	18-19	-0.06982873984200211
2207	20-21	-0.05114010392984625
2207	22-23	-0.0240395825760622
2207	24-25	0.009494346948930144
2207	26-27	-0.08921026545140265
2207	28-29	-0.06835850107044195
2207	30-31	-0.08479112994814386
2207	32-33	-0.041761787778288095
2207	34-35	-0.07506159541259194
2207	36-37	-0.014556204570641995
2207	38-39	-0.02692069177714984
2207	40-41	-9.279482380222248E-4
2207	42-43	-0.062360612005317506
2207	44-45	-0.055384311937707764
2207	46-47	-0.049846025365699376
2207	48-49	-0.036100463153037055
2207	50-51	-0.09234738095140571
2207	52-53	-0.01648346381310617
2207	54-55	-0.048645405157309085
2207	56-57	-0.06858832002135173
2207	58-59	0.001033679471046156
2207	60-61	-0.05119499608165867
2207	62-63	-0.048648942548545904
2207	64-65	-0.11432574645081672
2207	66-67	-0.12545551753765594
2207	68-69	-0.14517007261642334
2207	70-71	-0.11195555331152107
2207	72-73	-0.0925316074520488
2207	74-75	-0.11049573077859876
2207	76-77	-0.20276159347711342
2207	78-79	-0.19039799542092695
2207	80-81	-0.1657267178786732
2207	82-83	-0.1398333956597675
2207	84-85	-0.1694980906971466
2207	86-87	-0.16396227704018784
2207	88-89	-0.15996606758497833
2207	90-91	0.03965137240097505
2207	92-93	0.06471362198210784
2207	94-95	-0.0052651695579477575
2207	96-97	-0.02447671341168345
2207	98-99	-0.05292803086254949
2207	100-101	-0.317504603542055
2208	1	0.028306679484682462
2208	2	0.05956479768100209
2208	3	0.07532731887178556
2208	4	-0.02467557745944049
2208	5	-0.07450027635416845
2208	6	-0.008436489536933323
2208	7	-0.02119498434333522
2208	8	-0.03821155902597795
2208	9	-0.06269398885049782
2208	10-11	-0.05654665447778129
2208	12-13	-0.05989000824499513
2208	14-15	-0.042708422097859966
2208	16-17	-0.03322339870416613
2208	18-19	-0.07296078961051222
2208	20-21	-0.03914427362052919
2208	22-23	-0.12332051282937684
2208	24-25	-0.05509122893912277
2208	26-27	-0.014280158775751062
2208	28-29	-0.0692564084680285
2208	30-31	-0.04417808765723663
2208	32-33	-0.040125896134991734
2208	34-35	-0.031030129337018764
2208	36-37	-0.039894194979503084
2208	38-39	-0.10138856210019753
2208	40-41	-0.014309424992845265
2208	42-43	-0.024890245605966754
2208	44-45	-0.08135774450486366
2208	46-47	-0.14277890970991436
2208	48-49	-0.06077166434061354
2208	50-51	-0.034477514829927
2208	52-53	0.02111813520589223
2208	54-55	-0.030096976070211667
2208	56-57	-0.027114030457372706
2208	58-59	0.03207683534458283
2208	60-61	-0.016849241164688777
2208	62-63	-0.018792724619515866
2208	64-65	0.006814181525321317
2208	66-67	-0.027201602076967646
2208	68-69	0.0011893644965859096
2208	70-71	-0.021593659438860158
2208	72-73	-0.0341979110595112
2208	74-75	0.019465473246153664
2208	76-77	-0.046394473677136006
2208	78-79	0.019336702164924446
2208	80-81	-0.08162520439996968
2208	82-83	-0.12279121951477734
2208	84-85	-0.20884928087605914
2208	86-87	-0.3596062041577319
2208	88-89	-0.08637910286264372
2208	90-91	-0.993780535933837
2208	92-93	-0.26723742984468046
2208	94-95	-0.4391510258693856
2208	96-97	-0.5338388591234597
2208	98-99	-0.5674666924173444
2208	100-101	-0.677027788148628
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
2	91.0
3	0.0
4	0.0
5	0.0
6	1.0
7	4.0
8	1.0
9	4.0
10	19.0
11	20.0
12	36.0
13	57.0
14	112.0
15	185.0
16	315.0
17	488.0
18	667.0
19	878.0
20	1127.0
21	1933.0
22	2846.0
23	4426.0
24	7254.0
25	15604.0
26	147526.0
27	84255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.561095571182623	12.690042536664064	10.404490437653351	52.344371454499964
2	18.339437518900574	21.261606352833127	40.84540170021169	19.55355442805461
3	24.3623968130108	22.75060764115756	24.606199993279546	28.28079555255209
4	24.70272429615194	30.13376939992309	20.251335640603475	24.912170663321497
5	26.912278605776415	30.437785609748815	22.448552910606015	20.201382873868763
6	21.71820690015643	32.44626636649754	23.826857669806493	22.008669063539532
7	20.05719640543739	18.11617739845958	38.19876124234177	23.62786495376126
8	20.67097506430862	21.73762082367304	30.101661757184083	27.489742354834252
9	21.03797288770912	20.6063864341476	32.13340352213374	26.22223715600954
10-11	24.92953119108154	29.215714824397327	22.228755754174927	23.6259982303462
12-13	23.055154210021318	24.05403790941911	27.701242117760376	25.189565762799187
14-15	23.740988392713806	25.209166358657303	26.766200359157583	24.283644889471308
16-17	24.568320210267725	25.528936079656823	25.649339739928095	24.253403970147357
18-19	24.04209087956274	25.55880365429776	25.791957408838563	24.607148057300943
20-21	23.93419426617236	25.75256954478083	26.05945887421644	24.253777314830373
22-23	24.163987918566058	25.89910733286292	25.740809187265963	24.196095561305064
24-25	23.988702589892068	25.91833458403802	25.975642992880317	24.117319833189597
26-27	23.946327968370237	26.093246568029	25.94857550336197	24.01184996023879
28-29	23.988889262233574	25.78393049815381	25.775716915127557	24.451463324485065
30-31	23.98944927925809	25.67715391881247	26.00980403137589	24.32359277055356
32-33	23.93998110875904	26.05105861884868	26.071779248755828	23.93718102363645
34-35	24.114706420408513	25.96780275453707	25.658300012320375	24.25919081273404
36-37	23.890886282943	25.775530242786047	25.919454618087055	24.414128856183893
38-39	23.981422368573337	25.796064200351694	26.301572901149527	23.920940529925446
40-41	23.981422368573337	25.791397391814048	26.08111286583112	24.146067373781495
42-43	23.894806402114625	25.829665221822744	26.05553875504482	24.219989621017813
44-45	23.729228035198936	26.04489843157899	26.20394326654197	24.02193026668011
46-47	24.046010998734364	25.874839928467157	25.88977371578763	24.189375357010853
48-49	24.049744445564478	25.742302565998003	25.94857550336197	24.259377485075547
50-51	23.948381364126803	26.056285444410843	26.02343111230581	23.97190207915654
52-53	23.98758255584303	26.181169240878255	25.754062923512876	24.07718527976584
54-55	24.002329670821993	25.763956557612683	26.049005223092113	24.184708548473207
56-57	24.145694029098486	25.93812185223764	26.200956509077876	23.715227609585998
58-59	24.10443944162569	26.029031282550992	25.90638755418165	23.96014172164167
60-61	23.907500121337023	25.956415741705214	26.048818550750607	24.087265586207156
62-63	23.989262606916583	25.956975758729733	26.25397145406554	23.799790180288145
64-65	24.19852230174464	25.83377201333587	25.889960388129136	24.077745296790358
66-67	23.939421091734523	25.92654816706428	26.010737393083417	24.123293348117784
68-69	23.959021687592635	25.83750546016599	26.237170943330014	23.966301908911365
70-71	23.97992898984129	26.19087620263656	25.813424728111734	24.015770079410416
72-73	23.799976852629655	26.096606670176108	26.129087657598127	23.974328819596117
74-75	24.05739801156622	26.019324320792688	26.18191593024428	23.741361737396815
76-77	24.116013126799054	25.837318787824483	26.0995934276402	23.947074657736263
78-79	24.028463798632814	26.152981717310876	25.835452064409424	23.98310241964689
80-81	24.037984088049612	25.988336712102715	26.165115419508755	23.808563780338922
82-83	24.09286575645233	25.723635331847422	26.134501155501795	24.048997756198457
84-85	23.749388648081567	25.817531519624865	26.270771964801064	24.162307867492505
86-87	23.906566759629495	26.199276458004324	26.24613121572229	23.648025566643895
88-89	24.194788854914524	25.881373460419866	25.988710056785724	23.935127627879886
90-91	24.30641891513502	25.716915127553207	25.838438821873517	24.13822713543825
92-93	23.89331302338258	26.09231320632147	26.2431444582582	23.771229312037754
94-95	24.343006694070166	25.78803728966694	25.850945868754412	24.018010147508484
96-97	24.293351851229612	25.853745953877	25.936815145847103	23.916087049046293
98-99	24.295965264010693	25.91702787764748	26.050311929482657	23.73669492885917
100-101	24.65138940223783	25.744542634096078	25.755742974586425	23.848324989079668
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	2.0
16	5.0
17	7.5
18	10.5
19	15.5
20	20.0
21	28.5
22	42.5
23	63.0
24	126.5
25	228.0
26	385.0
27	545.0
28	720.0
29	1080.5
30	1474.5
31	1743.5
32	2015.0
33	2324.0
34	2747.0
35	3375.5
36	3996.5
37	4590.5
38	5365.0
39	6219.5
40	7284.0
41	8648.0
42	9680.5
43	10325.5
44	10906.0
45	11123.5
46	11325.5
47	12122.5
48	12960.5
49	13030.0
50	12518.5
51	11744.0
52	10781.0
53	10248.5
54	10433.5
55	9922.0
56	8785.5
57	7997.0
58	6901.0
59	5796.0
60	4967.0
61	4072.5
62	3639.5
63	3267.0
64	2561.5
65	2002.5
66	1561.5
67	1355.5
68	1222.0
69	1019.0
70	803.0
71	568.5
72	371.5
73	235.0
74	164.5
75	110.5
76	70.5
77	56.0
78	39.5
79	27.0
80	18.0
81	17.0
82	15.5
83	12.5
84	6.5
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029867574640935753
2	0.0
3	0.003733446830116969
4	0.0
5	3.733446830116969E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	267849.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.69408840068983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5623716290639	51.950409087115126
2	9.995611185249079	12.733226849043577
3	3.1947144335386772	6.104532706343167
4	1.4905270052149588	3.797510353351082
5	0.8725392208870194	2.778779513412342
6	0.55360835935223	2.115694786796508
7	0.38537193434967776	1.718213983752925
8	0.314619574221819	1.603152557845755
9	0.2033036957962869	1.1654319215012086
>10	1.4223398099582591	15.850860027094066
>50	0.0049931523680946535	0.18218821374420768
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTGG	50	0.0016122013	38.00672	3
CCGGGAA	65	0.0058204937	29.246862	1
CTCGCCA	65	0.0058204937	29.246862	1
CCCGCTT	70	0.008357804	27.157803	1
AACAATT	125	0.006013185	19.00336	2
CTGGGAT	205	3.3062082E-4	16.228443	1
AGTTCAA	195	0.0044498937	14.615241	5
GAGTAGT	125	0.0014974214	13.29987	30-31
AAGTTCA	220	0.009890434	12.954418	4
CTGAAAT	275	0.003044474	12.097567	1
CCCAGCT	405	3.2130985E-5	11.734852	1
AGGGTGT	145	0.0045575905	11.465405	66-67
CTGAATT	335	0.001024679	11.349529	1
CTTGAAT	295	0.0051441877	11.277392	1
GAATGTC	425	6.555659E-4	10.058724	4
GGTAGTA	190	0.002949709	9.999902	52-53
CTCTGCC	390	0.0036302158	9.748955	1
CTCCTTT	535	5.356131E-4	8.883393	1
CTTTGCT	500	0.0028739348	8.5547085	1
>>END_MODULE