# HG changeset patch # User iuc # Date 1561040328 14400 # Node ID acd337269171d9436dcb4b45e1342ecbbe133be4 # Parent 4c172a4a4c9e39a1096bcdb91cc2a4d0610f7b5f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/nanoplot/ commit fae386db359dd61fd5e4e2e808413ae478b279ee diff -r 4c172a4a4c9e -r acd337269171 nanoplot.xml --- a/nanoplot.xml Tue Oct 23 08:30:58 2018 -0400 +++ b/nanoplot.xml Thu Jun 20 10:18:48 2019 -0400 @@ -1,8 +1,12 @@ - + Plotting suite for Oxford Nanopore sequencing data and alignments + + 1.25.0 + - nanoplot + nanoplot + NanoPlot --version - - - @@ -408,12 +409,10 @@
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- - + @@ -429,14 +428,30 @@ - + + + + + + - + + + + + + + + + + + + +

NanoPlot report

Summary statistics prior to filtering

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General summary:
Active channels:169
Mean read length:23212.6
Mean read quality:10.1
Median read length:9390.0
Median read quality:10.3
Number of reads:371
Read length N50:60395
Total bases:8611871
Number, percentage and megabases of reads above quality cutoffs
>Q5:371 (100.0%) 8.6Mb
>Q7:371 (100.0%) 8.6Mb
>Q10:207 (55.8%) 4.7Mb
>Q12:49 (13.2%) 0.9Mb
>Q15:0 (0.0%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1:13.3 (6333)
2:13.1 (5448)
3:12.9 (27834)
4:12.8 (20351)
5:12.8 (97730)
Top 5 longest reads and their mean basecall quality score
1:393431 (10.6)
2:341725 (11.8)
3:320662 (8.0)
4:226843 (9.1)
5:221901 (10.0)
+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
feature
General summary
Active channels169.0
Mean read length23,212.6
Mean read quality10.1
Median read length9,390.0
Median read quality10.3
Number of reads371.0
Read length N5060,395.0
Total bases8,611,871.0
Number, percentage and megabases of reads above quality cutoffs
>Q5371 (100.0%) 8.6Mb
>Q7371 (100.0%) 8.6Mb
>Q10207 (55.8%) 4.7Mb
>Q1249 (13.2%) 0.9Mb
>Q150 (0.0%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
113.3 (6333)
213.1 (5448)
312.9 (27834)
412.8 (20351)
512.8 (97730)
Top 5 longest reads and their mean basecall quality score
1393431 (10.6)
2341725 (11.8)
3320662 (8.0)
4226843 (9.1)
5221901 (10.0)
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Summary statistics after filtering

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General summary:
Active channels:169
Mean read length:23212.6
Mean read quality:10.1
Median read length:9390.0
Median read quality:10.3
Number of reads:371
Read length N50:60395
Total bases:8611871
Number, percentage and megabases of reads above quality cutoffs
>Q5:371 (100.0%) 8.6Mb
>Q7:371 (100.0%) 8.6Mb
>Q10:207 (55.8%) 4.7Mb
>Q12:49 (13.2%) 0.9Mb
>Q15:0 (0.0%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1:13.3 (6333)
2:13.1 (5448)
3:12.9 (27834)
4:12.8 (20351)
5:12.8 (97730)
Top 5 longest reads and their mean basecall quality score
1:393431 (10.6)
2:341725 (11.8)
3:320662 (8.0)
4:226843 (9.1)
5:221901 (10.0)
+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
feature
General summary
Active channels169.0
Mean read length23,212.6
Mean read quality10.1
Median read length9,390.0
Median read quality10.3
Number of reads371.0
Read length N5060,395.0
Total bases8,611,871.0
Number, percentage and megabases of reads above quality cutoffs
>Q5371 (100.0%) 8.6Mb
>Q7371 (100.0%) 8.6Mb
>Q10207 (55.8%) 4.7Mb
>Q1249 (13.2%) 0.9Mb
>Q150 (0.0%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
113.3 (6333)
213.1 (5448)
312.9 (27834)
412.8 (20351)
512.8 (97730)
Top 5 longest reads and their mean basecall quality score
1393431 (10.6)
2341725 (11.8)
3320662 (8.0)
4226843 (9.1)
5221901 (10.0)
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Plots

Histogram of read lengths

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Histogram of read lengths after log transformation

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Weighted Histogram of read lengths

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Weighted Histogram of read lengths after log transformation

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Yield by length

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Read lengths vs Average read quality plot using hexagonal bins

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Read lengths vs Average read quality plot using a kernel density estimation

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Number of reads generated per channel

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Cumulative yield

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Cumulative yield

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Number of reads over time

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@@ -338,7 +380,7 @@

Number of active pores over time

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@@ -346,7 +388,7 @@

Violin plot of read lengths over time

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@@ -354,7 +396,7 @@

Violin plot of quality over time

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diff -r 4c172a4a4c9e -r acd337269171 test-data/NanoStats.txt --- a/test-data/NanoStats.txt Tue Oct 23 08:30:58 2018 -0400 +++ b/test-data/NanoStats.txt Thu Jun 20 10:18:48 2019 -0400 @@ -1,27 +1,6 @@ -General summary: -Active channels: 169 -Mean read length: 23212.6 -Mean read quality: 10.1 -Median read length: 9390.0 -Median read quality: 10.3 -Number of reads: 371 -Read length N50: 60395 -Total bases: 8611871 -Number, percentage and megabases of reads above quality cutoffs ->Q5: 371 (100.0%) 8.6Mb ->Q7: 371 (100.0%) 8.6Mb ->Q10: 207 (55.8%) 4.7Mb ->Q12: 49 (13.2%) 0.9Mb ->Q15: 0 (0.0%) 0.0Mb -Top 5 highest mean basecall quality scores and their read lengths -1: 13.3 (6333) -2: 13.1 (5448) -3: 12.9 (27834) -4: 12.8 (20351) -5: 12.8 (97730) -Top 5 longest reads and their mean basecall quality score -1: 393431 (10.6) -2: 341725 (11.8) -3: 320662 (8.0) -4: 226843 (9.1) -5: 221901 (10.0) +General summary: +Mean read length: 2,564.9 +Median read length: 1,910.0 +Number of reads: 9.0 +Read length N50: 4,275.0 +Total bases: 23,084.0 diff -r 4c172a4a4c9e -r acd337269171 test-data/NanoStats_post_filtering.txt --- a/test-data/NanoStats_post_filtering.txt Tue Oct 23 08:30:58 2018 -0400 +++ b/test-data/NanoStats_post_filtering.txt Thu Jun 20 10:18:48 2019 -0400 @@ -1,12 +1,12 @@ -General summary: -Active channels: 169 -Mean read length: 23212.6 -Mean read quality: 10.1 -Median read length: 9390.0 -Median read quality: 10.3 -Number of reads: 371 -Read length N50: 60395 -Total bases: 8611871 +General summary: +Active channels: 169.0 +Mean read length: 23,212.6 +Mean read quality: 10.1 +Median read length: 9,390.0 +Median read quality: 10.3 +Number of reads: 371.0 +Read length N50: 60,395.0 +Total bases: 8,611,871.0 Number, percentage and megabases of reads above quality cutoffs >Q5: 371 (100.0%) 8.6Mb >Q7: 371 (100.0%) 8.6Mb diff -r 4c172a4a4c9e -r acd337269171 test-data/bam-LogTransformed_HistogramReadlength.svg --- a/test-data/bam-LogTransformed_HistogramReadlength.svg Tue Oct 23 08:30:58 2018 -0400 +++ b/test-data/bam-LogTransformed_HistogramReadlength.svg Thu Jun 20 10:18:48 2019 -0400 @@ -1,68 +1,40 @@ - - + + - - - - - - - - - - - - - - - - - + +" id="DejaVuSans-48"/> - - - + + + + + + + + + + + + + + + + + + + - - - - - - + + + + + - - - - - - - + + + + + + + + - - - - - - - - + + + + + + + + + + + + + + + + + + + + + + + - + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +" id="DejaVuSans-82"/> +" id="DejaVuSans-101"/> +" id="DejaVuSans-97"/> - +" id="DejaVuSans-100"/> + +" id="DejaVuSans-108"/> +" id="DejaVuSans-110"/> +" id="DejaVuSans-103"/> +" id="DejaVuSans-116"/> +" id="DejaVuSans-104"/> - - - - - - - - - - - - + + + + + + + + + + + + - - + - - - - - + + - - - + - - + - - - - - - - - - - + + + + - - + - - - - - - + + + + + - - + - - - - - - - - - + + + + + - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - + + + + @@ -590,7 +573,7 @@ L 19.390625 0 L 9.8125 0 z -" id="DejaVuSans-4e"/> +" id="DejaVuSans-78"/> +" id="DejaVuSans-117"/> +" id="DejaVuSans-109"/> +" id="DejaVuSans-98"/> +" id="DejaVuSans-114"/> +" id="DejaVuSans-111"/> +" id="DejaVuSans-102"/> +" id="DejaVuSans-115"/> - - - - - - - - - - - - - - - + + + + + + + + + + + + + + + - - - - - - + - + + + + + + + + + + + + - - + - - + + + + - + +" id="DejaVuSans-72"/> +" id="DejaVuSans-105"/> - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - + + + + + + + + + + + + + + + + + + + + + + + + + + - - + + diff -r 4c172a4a4c9e -r acd337269171 test-data/bam-report.html --- a/test-data/bam-report.html Tue Oct 23 08:30:58 2018 -0400 +++ b/test-data/bam-report.html Thu Jun 20 10:18:48 2019 -0400 @@ -32,8 +32,7 @@ NanoPlot Report
-

Summary Statistics prior to filtering

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Summary Statistics after filtering

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Summary Statistics

Plots

Histogram of read lengths

Histogram of read lengths after log transformation

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Aligned read length vs Percent identity plot using a kernel density estimation

NanoPlot report

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Summary statistics prior to filtering

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Summary statistics

+
General summary:
Average percent identity:85.3
Mean read length:12291.0
Mean read quality:13.2
Median percent identity:87.3
Median read length:7577.0
Median read quality:13.5
Number of reads:1115
Read length N50:21987
Total bases:13704509
Total bases aligned:12343518
Number, percentage and megabases of reads above quality cutoffs
>Q5:1115 (100.0%) 13.7Mb
>Q7:1115 (100.0%) 13.7Mb
>Q10:1098 (98.5%) 13.6Mb
>Q12:936 (83.9%) 11.9Mb
>Q15:10 (0.9%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1:15.7 (1461; SRR5665597.758)
2:15.5 (1790; SRR5665597.725)
3:15.5 (615; SRR5665597.293)
4:15.5 (162; SRR5665597.876)
5:15.5 (626; SRR5665597.822)
Top 5 longest reads and their mean basecall quality score
1:99139 (13.9; SRR5665597.31)
2:80522 (13.5; SRR5665597.790)
3:79569 (13.5; SRR5665597.332)
4:70195 (10.2; SRR5665597.799)
5:69426 (12.2; SRR5665597.1000)
+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
feature
General summary
Average percent identity85.3
Mean read length12,291.0
Mean read quality13.2
Median percent identity87.3
Median read length7,577.0
Median read quality13.5
Number of reads1,115.0
Read length N5021,987.0
Total bases13,704,509.0
Total bases aligned12,343,518.0
Number, percentage and megabases of reads above quality cutoffs
>Q51115 (100.0%) 13.7Mb
>Q71115 (100.0%) 13.7Mb
>Q101098 (98.5%) 13.6Mb
>Q12936 (83.9%) 11.9Mb
>Q1510 (0.9%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
115.7 (1461; SRR5665597.758)
215.5 (1790; SRR5665597.725)
315.5 (615; SRR5665597.293)
415.5 (162; SRR5665597.876)
515.5 (626; SRR5665597.822)
Top 5 longest reads and their mean basecall quality score
199139 (13.9; SRR5665597.31)
280522 (13.5; SRR5665597.790)
379569 (13.5; SRR5665597.332)
470195 (10.2; SRR5665597.799)
569426 (12.2; SRR5665597.1000)
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Summary statistics after filtering

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General summary:
Average percent identity:85.0
Mean read length:1436.3
Mean read quality:13.1
Median percent identity:86.8
Median read length:1419.5
Median read quality:13.4
Number of reads:80
Read length N50:1474
Total bases:114904
Total bases aligned:110215
Number, percentage and megabases of reads above quality cutoffs
>Q5:80 (100.0%) 0.1Mb
>Q7:80 (100.0%) 0.1Mb
>Q10:77 (96.2%) 0.1Mb
>Q12:64 (80.0%) 0.1Mb
>Q15:3 (3.8%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1:15.7 (1461; SRR5665597.758)
2:15.5 (1790; SRR5665597.725)
3:15.1 (1474; SRR5665597.822)
4:14.7 (1985; SRR5665597.268)
5:14.6 (1726; SRR5665597.119)
Top 5 longest reads and their mean basecall quality score
1:1985 (14.7; SRR5665597.268)
2:1981 (13.2; SRR5665597.912)
3:1969 (13.2; SRR5665597.827)
4:1955 (13.2; SRR5665597.213)
5:1930 (11.1; SRR5665597.21)
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Plots

Histogram of read lengths

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Histogram of read lengths after log transformation

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Weighted Histogram of read lengths

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Weighted Histogram of read lengths after log transformation

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Yield by length

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Read lengths vs Average read quality plot using dots

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Read lengths vs Average read quality plot using a kernel density estimation

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Aligned read lengths vs Sequenced read length plot using dots

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Aligned read lengths vs Sequenced read length plot using a kernel density estimation

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Read mapping quality vs Average basecall quality plot using dots

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Read mapping quality vs Average basecall quality plot using a kernel density estimation

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Read length vs Read mapping quality plot using dots

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Read length vs Read mapping quality plot using a kernel density estimation

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Percent identity vs Average Base Quality plot using dots

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Percent identity vs Average Base Quality plot using a kernel density estimation

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Aligned read length vs Percent identity plot using dots

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Aligned read length vs Percent identity plot using a kernel density estimation

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diff -r 4c172a4a4c9e -r acd337269171 test-data/reads1.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads1.fasta Thu Jun 20 10:18:48 2019 -0400 @@ -0,0 +1,10 @@ +>570cfbe8-1011-441d-911f-d98dcc4472fc_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2408 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode01/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2408.fast5 +TTGTTGTACTTCGTTCCGGTTACGTATTGCTTCAGAAAGTTGTCCGGTGTCTTTGTGGTTTCATCGTGAAACGCCTTCGCGTTTTTTCGTGCGCCGCTTCACTGGTTTCCTGTGGCTTCGCGGCTGGTTTTACTTGCTTTGTTATAAACCATTTGCCCGCTTCGACATCAGCGGTCAGCGTTTGAACTCATGCGCGAACAGCCCCAACAACTCTTCGCTGCCGGTTACTGCACTTCCAGTTGTTCACAAGGTCAGCTCAAGTTCAACGTCGGTTTTAATCGTCGCCAGCTGGTATGAGATAAACAGCTTCTTTGTTTTTTGCTCGAGCTTCGCTGCCGTGATTTTCGCGCCACGGAAGCTCAACCCGAAAACCGTTTTTCTGGCTCGGCATACAGCGTATCAGTCGAACCTT +>b9497173-8976-48fa-a822-93edea3f0baf_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2617 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode01/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2617.fast5 +TGGTATGCTTCGTTCAGTTACGTATTGCTAGAAAGTTGTCCCATTGTCTTTGTGGTTTTCGCATTTATCGTGAAACGCTTTCGTTTTTCGTGCGCCGCTTCAATTCGTGGTGGCTTTCAGGGCACTCGCGCCGGATTTAGCCGCTAAAATGACGTCCATGCGTTCGTGTGCATAGTATATGCAGACAGAAGAAATCATCAGGCGCGTTTTCACGATATGGACGGGAAAGATGCCGGAAATAGGCAGGAGGACGCAGAAATGCCCTAGGGAGTCTGAGATGGTGCGGGCAACGGATATTATATTAATATACGTTGGTAAGTGTACCAATGCAAGTTTTCAAGTTTTGAGACTCGTTTAGAACATCTAAAAAAAAACACATGAATGCTGCTATAATGAAGCGACTGTTTGATGGTTCATGGTTTACCAGTAGATGAAAACTCAATACAGCGCTATTTAAGAACGCTTAAAATGTACATTCTTGTATTTTTAACGTGCAGAGTAACAGGCTCTAGCTGGTTATGGTACCTCGAAGTTCCTTAGCAAAACAAAGGGACTTTGAAGCTACGCAACCGCCAAGAAGCTCGCTTCAAAGTCCCTGTACCGCAGCTATATTGCATGGGTTTGGTTAAGACATACGTCATGTCTTTGGGGGGCATATTATGGTGAAAAGCTCGCGTCTACACCAATATTTATACATTTCTCTTCAATATTGACGCCGTTCCAGCCAACTTTTGCTATATGGGTGACAATTTTCGCTTTTAAACGGAGATGTCTCCATGATTGAGCATGATCCTAAAATCTGCGTTTTATTGCTGACAGGCATTACCATTCTTTCTATGCTGGAAAACGTGCCGCGTCTTGCATTCTGCTTTTGGCAGTGCGGGAGTCTCAATGAGCTTTAACTACTCCCCTCATAACGCCGTTCGTGTTTTAAGCCGCCTTCTCGATAAGAGTTTCTGAGTGCTGAAGAAATTCGCCTTTACGATAGGTAGAGACGTTGATTGAGAGTGCATCAACAACAGGCTTCTTGTTCCTACGAGCATGAAGATCAGCCGCGATGGTGAGTATGTTCGTGTCTGGTATTTGTCTCAGAAGCCATTGATGACTATTACAACCGTCAGTAGTATCAGTTTAGTCAGCTTGAGGCATGCTGTCATGTTGCTCATCAGCATAGAAGAAGCCTCTACGACCGCTCGTTGCTTTTATCGCTGGGTGAAACTGTCAGAAAGTATCCGTATGATTCTTGTATGATGATTAGGAGGATATTATGTTTCTGGGTTATTTGTTATTTTGCTGGCTTTCTCTACCTATTGAGCTTGGGGTTTCTTCTGGAAACATTGAATGAAGTGCCTCTCATAGATTTGTTTTGGGCTTCATCTGGTCTATAAAACAGTCAGGGAGAGAAGAGTGCTTAAACTTTGATGTTTAGGTCTTAAACTAGACTAAAAAAAGCTCATAATATGCGAGCTTTTATTTATTCATAGACTTGTGTAGTTTTCACTCTTTTTTGCACGGTGTTTCTAAGCCACAAAAAAAAACTTCTGCCTGACCGCCTGTTTGTGGTTGCCTGTTATAAAGTAACAGTAAAAAAATCATGCGTTTCGCAGGAGAAAATATGAGCAAGAGCTATCATAAATCGGCATATTTCCAATAGAGTTCCGTCCTCTTTGAAAGGGGTAGTTCATGAAAAGAGTGGTTCATGGAAGGAAATGTTTATGATTTGGAGTTAGGTGTAAAGGCGCAAAGAAAATATAAGAGATAGTGAAATTCATCATTAAAATGGTGATTGAGAGGCATTATAGAGCACAACTAGGGAAGTTAAAGGAGGAAAAAAGAGCACTCCAATGGTGCCAATGAAGATATTTCTGGTCTGATAGTATAGATCAGATTACATCTAGGCAATCCATGGATATTATAGGTTTCTGTGGATGTTGGTTCCGGTATTAAGTCAATTGTAGATGTAACCGAAGGAAAGAACTGGAAGATTTAGGATACACGAAGTATATGGGATAAGAGTTAGCGATCATTATGCAGAAACTTTTTCTGAGTGAACGGGGATACTCCATTCCAAAGTATAGTCCTTGCAGGATTTTGGTGATTCATATCAAAGATGTAAGTACTACCCACATGCTGTATGAAAGTGCATGAAATAAAGATAGAGAAAAAAAATCTGGAAAATCAAAAGAGTTAAAGAAACGAGCTTTCCTTATGAATCAATAAGCACAAGATCAGTGAGTTGCTTAGAATGATTTTCCATCATAATTTTGTATTAGGTGTTATTAGGAGCGAGACTGAAAGAAAGAAATCTTCGAAGTGGGGGGTTTAACCAAATCTGAAGTTGATATAATCATCAGATCATGACAAAATTTGAGCACCACTTATGGCCAGCAACAGCTATACTTGATGCTGACTTCTTGCATTAAAATAATCAAGTGAAGAGCTCACCTTAAATCTAAGTTAAACGCTTTCTTAAAAGTTCTAATTCCTTGGGCTTAATGGCCGCCAAACACTTGCATGAAGGGTATGTACGCAGCTCATTCTGCCTCTTTACAATCTGCATGTCTTCCAGACAGGTAGGTGCTGCTATTTAGATATAAAGGAAATTTAATTGCTGTTGGGCATGTTCCTAAATTGCAGTGGCCTTTTATAGTGCTGATGATGGAGTGAATGATCATCGCTGTGTCTACAAAGGATGGCAAATGTTACAATGATAAGAAAATTAAGAAAAGATAAAATACAGCAAATGGCCTTTCTGATAAGGTTAAGGGAGTTGTCTGTTCAGTTAACTCAGAACAAGCGAAAAGGTTGCTGAATAATCTACAGGCTGCTCTGTATCATCAATCATTGATACTCTCGCTCTATTCATGCTACTGAAATGGATGCTATTACATCTCTTGTAGACTCGGGAATGGTGGTTTTTTGAAGATAGTTTTATATACAGCTACGTTTCCCCTGTCATAGTGTGCGAGAACATATTGTTGCTGTTGGTATCAATAAGTTGTTTACATTGAACATATGAAAAGAAGCAGCTTTAGAGTTGCACGATGATGCATGCTATGATTAACGAAAGTGAGTTTTATTTGGAGTCCTTTGAAGGGGTTTCACCAAGGAGATATCATAAGTTCTTCTTTTCTACAGATGAAAGAAGGATAGTAAGGTAATGCGGAGAAATATTCAACGAAGTATATCATATTGATATACAGTTTATTGATAGTTATGTGGATTTTTTTTGAAAATCGTGTGGCGGATATTTTCATTAGTAACACAAAAACAGATGCTGAAGCTCAAGTAGCCGTTCCATCTGGGACGCCAGTTACGCTTCTGCCACCGATTCACTCCGACGCCACCAACATCGGTATGAAGATCTATTGACTCTGATGTTACCTGAAATGGCTTGTCTTGAAAAAAAACCACCGCTACCAACGGTGGTTTTCAGGATTCTCTGAGCTACCAACTCTTTTGAACCGAGGTAACTGGCAGACTCAGTCACCAAAACTTGTTCTTTCAGTTTGCTTTATACCAGGCGCACAACTAAGACAACTCCTAAATCAGTTACCAGTGGCTGCTGCCGAAGTGGCGTTTTAATCTCGTGCCTTCTCAGGTTGGACTCAAGACAATGGTTACCGGGGCAGGCGCAGCGGTCGGACTGAACGGGGTTCGTGCGCCTGTCCAGCTTGGAGCGAACTGCCTACCGGAACTTTAGTCAGGCGTGAATGAGACAAACGCGGCCATAACAATGACACCGATGCCAAGCCGAAGAGCGGGAACAGGAAGGCGCACGAGGGGGGAGCTACCGGGGAAACGCACGGGGATCTTTACTCGTCGCCGCCACCGCACTGGTGAGCGTCAGATTCTGTGATGCTTGTCGGGGGGGCCGGAACAATATGGAAAACTCAGCAGCATACCCGGCCTTTCTTGTTATACTCCATTCTCTCTCATTGCCTATGGCAATGTCTCTGTTCCCACTCGCTCGCTGCAGCCGAACGACCGAGCCGGAGCGAGTCAATGTTGAGGAAGCGGAATATCTGCGGGCTTCTCTTTGGCACCGTACGCGCCATGTGCATTTTTTTTAATACGATGCAGGATAGGGCGAACGCCATAAAGTGACGTCACCTGACGTTCTGATTACAAAGGTTAAAAGCAGCGGACGGGAATGTTTGTGCCCCAATATGCTGGCTTATTCACAAAACAACCCGGGACAAGATGTGTTTTTTAGTGCCGCCGGCCCGCTAAGACTCTGGTTAGCTCCCGCATGTTTGCTTTATCTTTTGCTACCATTTTGCTACTTTTTTGCTATCTCAGAGTCTTCTGGTTTCGGTTACTATCCTTTTGCTACTTTTGCTATCAAAATGCTACCTCTCCCTTCTTGCAATAATGACCAAGGCACGTTGGCGATGTATGCATCGCCGCAAGTGCTTGCTTCCGAGATCCGTCG +>4850ae22-4e87-4dbf-83ee-c13cc2c33f9e_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2911 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode01/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2911.fast5 +TTTGATGTGCTTCTCGGATTACGTATTGCTAAGAAAGTTGTCGGTGTCTTTGTGGTTTTCGCATTTATCGTGAAACTAACTCGCGTTTTTCGTGCGCCGCTTCACTTGCGGCGCGGCGATGTATACATCGCTAACGTGCTATGATCATTTATTGCAAGAAGGAGGAGTGGCATTTTGATAGCAAAAAAAGTAACAAGGATGATAGCAACCGAATGAAGACTCTGGAGATAGCAAAGTAGCAAAATGGCAAAGATGTAAACATGCGGGGCAACAGGGGAGTCTTTAGCGGATACGGCGGTACTCAAAATACATCTTGTCCCGCTGTTGTTTTGGCCAGAATAAGCCAGCATAGCTGTAGATTTCGTATTTCCTGTCCGCCTGCTTTAACCTTTGTAAATCCGGGAACGTCAGGTGACGATCACTTTGCGGCGTTCGCTATCACCATCGTATTAAAAAATGCAGCTATGGCGTACGGTGCAAAAAAAACCCGCAGATATTCCAGCTGCTCGCTCACTGACTCGCTCGCTCGGTCGTTCGGCTGCGGCGGCGGAGGGCTGAGACATTAAGCCATAGGCAATGAGAGAGAGAATGGAGAAGCAACAAGAAAGGCCGCGGCGTTGCCACGGTTTTCCATGACTCCGCCCCCCCCTGACAAGCATCACAGAATCTGACGCTCCAAATCGGTGGTGGCGAAACGACAGGTTATAAGATCGAGGCGTTTTCCCCTGGTAGCTCCCCTCGTGCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCCTTCCGCTGTTATGGCCGCGTTTGTCTCATTCCACGCCTGGCCTCAGTTCCGGGCCAGGCGGTTCTTCGCTCCAAAGCGGACTGTATGCTGAACGGTCGAGATCCGACCGCTGCGCCTGCGGTAACTATCGTCTTGAGTCCAACCCGAAGGCTGGCAAGCGCAACCACTGGCAGCCACTGTTAGCTGATTTAGTAGTCTTGAAGTTTGTGCGCCTGTTAGGCTAAACTGGCTGGATTTTGACTGAAGAACCATCCAAACCGGTTACCTCGTTCAAAAAAAGTTGGTAGCTCGAGAACCTTGAAAAACCACCGTTGGTAGCGGTAGTTTTTTCTTTAAAAGACAAGATTAGCTCAATGTAACATCAGAGTCCAATAGATCTTCATACCGATGTTGGCGTCGGAGGAATCGGTGGCAAGCAGTTAACTGGCGTGCGATGGAACGGCTGCCCGAGCTTCAGCATCTGTTTTTTGTGGATCACTAATAAGAAAATATCCGCCACACGATTTTCCACCACATAACTATCAACCAAACTGTATATCAATATGATTCTTATACTTCGTTGAATATTTCTCCGCATTACCTTTACTATCCTTCCTTTTCATCTGTAGAAAAGAAGAACTTATGATATCTCCTTGGTGAAACCCTTCAAAGGACTCAAATAAAACCTTACCTTTCGTTAACTTCAATTGTGCATCATCGTACGCTCAAGCTAAGCTTTTTTCATAAGGTTTAATGTAAACAACTGGGAAAAATCAACAGCAACAATATGTCTCGCACAATTATGACAGAACGTAGTTTGTATATAAAACCTTATCTTCAAAACCTGTTCAGGTCTAGCAAGAGATGTAATAGCATCATTTTCAGCATGAATAGAGCGAGTACTCAATGATTGATGATACAGAGTGCCTTCGTAGATTATTCAGCAACCTTTTCTGCTTGTTCTTGAGTTAACTGAACAGACAACTCCTTAACCTTATCAGAAAGCAATATTATTTGCTGTATTTTATCTTTATTTTTAATTTTTCTTACTTCATTCATGAAACATTTATACATTTTGTGAACACAGCGATGATCATTCCTTGCATCATCAGCACTATAAAGGCCGCAATTAAATTCGTATCATTGCGCCCAACAACAGAATTAAATTTCCTTTATATCTAAAATAGCAGCACCTACCTGTCTGGAAAGACATGGAATTGTAAAGAGGCGGAATGAGCTGCATGCCCTTCTCATGCTGGTTTGGCGTAAAGACCATTAAATGCTGTAATTAGACCAAGCGTTTAACTTTAGATTTAAGGTGAGCTCTCTGGCCTTGATTATTTTTAACAAAATAGTCGGCATCAAACTTTTTTATGTTTTGCTGCAATATAAGGGTGCTCAGACTTCCTGTCATGATGGATAGATTATATCAACTTCAGATTTGCTTAAACCCTCATCTCGAAGATTTCTTTTTCTTTTCAATTCTCGCTCTATTAACACCTAATAAATAGAAGTATGTTGATAGACCATTCTAACAGCACAATTTCATCTTGGTGCTTTAGTTGATCTATGGAAAGCTCGTTTCTTTAACTCTTTTTGATTTTTCCTTCTAGATTTTTTTTCTATCTTTATTTCATGAATTACAGCTTCAGCTAGTATGTGTGCGTTAGTACTTACATCTTGAGAATATAAAATCCTGCAAAGGAATTATATCTTTTGAATAGATCCCACACCACTCAGAAAAAAAAGTTTCTGCATAAGATCGCTAACTCTTATCCTATACTTCGTATCCTAAATCTTCCGTTCTTCTTCGGTTACATCTACAATTGTTAATACCGGAACCAACTTATTACAGAAACCTATAATAATATCCATGGATTGCCCTGGAGTAATAATCTGATCTATACTATCACGACCAAATATCTTCATTGGCTTTTCTCATTGGGTGCTTTCTTTTTTTCCTTTAACTTCTAGCTGTGCTCATAACGCCTCTCAATCCACCATTTAATGATTTAGAATCTTCAATTATCTTTATAACTTCTTCTCTTTTGCACTTATATGCTCCATCCAAATCATAAACATTTCCATGAACCACTCTTTCTTGAACTACCCTCTTTCAAAGGACGAAACTCGTGGAAATACGATTTAGTGATAGCTCTTTTAACATATTTCTCCTGCGAAACGCATGGATTTTACTGTTATCTTTATAACAGGCAACCTGAAGCTGAGCGTAATACTGAAGTTTTTGCTTAGAATTGCAGGAAGAGTGAAGAAACTACCTGGTTGAACATACTTTCACCATCGCGAAACTGATCTTCATGCTCAAGAGACAAAGCCTGTTGATATATAATCACCCGCCCTTGATCATCAGCGGGTTCTTCAGCACTCAGAAACTCTTTATCAACGGTTAAAAGAAACACGAACGGCGTTAGCATAACAGCCAGTTAAAACTCTCATTGACACCTCCGCATACAAAAGCAGAATGCAAGACGCGGCACGTTTGTGCATAGAAAGAAAGAATGGTAATGCTGCAGCTGAAATGCAGATTTTTAGGGATCGCTTAACCAATCATGGAGACATCTCCATTTTAGAAACTCTTGAAATTGTCACCCATATAGCAAAGTTGGCTTTCAAATCAATATTGAAGAGAAATATATAAAATATTGGTGTGACATGACGCGTTTTTTCACCATAATATGCCCCCAAGACATGACGTGTCTTAGCAAACCCATGCAATATGAAAGCTGCATTTTAGAGGTTTTGGCTTGAGCTTCTTGGCGGTTTGCGTAGCTTCAAGTCCACTTTGTTTCATCCCTATAAAGGGAACTTTTCCAAGTGTACCAACTAGCTTCAAGTACATTGCACTGCATGTTAAAGCCCGCAAAATGCCATTTAAGCGCCTAAATAGCGCTGTGTTGAGTTTCATCTACATAATGGTAAAACCATGAACAAAACAGTCGCTGTATGGCCCGGCATTCATGCTTTTTTTTTTGAATGTTCTAAACAAACCCTGAAAAAAAACTTGCATTGGCATGCCCGCAGCAGGCTATTTACCATAACATCCGTTGCCCGCATATCTCAGACTCCCTCAGGGCATTTCCGTCCTCTGCTATTTTCGGCATCTTCCCTGTCCGTGAAAAAACGCGCTGATGATGATTTCTTCGCGTCTGCGCAATATGCACACGAACGCATGGACGATCATTTAGCGGCTAAAATCGGCGGCGAGCGCCCTGAAGCTACCACGAACCCGCCGATGGCGGTGAAAGGGTGCGCAGTTTACTGCGGCCGTGAAGAAGAGGCGTTAGCCCGGGCATCAGGCATGGCGGGATTTATGGGGTCGGATTTTAACCTGTCAGCACAGTGATGAACGGG +>bd2c355e-c70e-42c7-a02f-810dd87fab35_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch101_read10963 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode01/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch101_read10963.fast5 +TGGTACTTCGTTGATTACTAAGAAAGTTGTCGGTGTCTTTGTGGTTTTCATTTATCGTGAAACGCTTTCGCGTTTTCGTGCGCCGCTTCATGGAGATAGCAAAAAGAAGCCAGCAAAATAGTATAAAAGATAGCAAACATGGAGCTAACGAGTCTTTAGCGGGCCGGCGGTACTCAAAACACATCTTGTCCACTGTTTTGTGAATAAGCCAACATAAGCTGTGGGCACAAAACATTTCCTGTCCGCTGCTTTAACCTTTGTAATCCGGGAACGTCAGGTGACGTCACTTTGCGGCGTTCGCCCTATCTGCATCATCATTAAATGGCTATGGCGCGTACGGTGCCAAAGAAAGCCCACAGATATTCAACTTCCTCGCTCACTGACTCGCTCCGCTCAGTGGATTTCGGCTGCGGCAGTGGCGGAGGTCAGGAACGATAAGCCATAGGCAATGAGAGAATGGAGAAAGCAACAAAAGAAAGGCCGCGAAAGGTTGCCGGTTTTCTATAGGCTCCGCCTGACAAGCATCACAGAATCTGACGCTCAAATCAGTAGTGGCGAAACCCGACGGACTATAAGATCGAGGCGTTCCCTGGTAGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCCTTCCGCTGTTATGGCCGCGTTTGTCTCATTCCACGCCTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTATGCACGAACCCCCCCGTTCAGTCCGACCGCTGCGCCTGCTGGTAACTATCAGTCTTGAGTCCAACCCGAAGGCACGACAAGCCGCGGCGGCAGCCACCTGGTAACTGATTTAGAGTTAGTCTTGAAGTTGTGCGCCTGTTAAGGTAAACTGAAAAAGAACAAGTTTTCAGTGACCTGGCTCCTCCAAGAAGCCAGTTACCTCGGTTCAAGTTGGTAGCTCAGAGAACTGAAAAAAACCACCAGACGGTAGCGGTGGTTTTCTTCTTTAAAGACAAGATTAGCTCTGGCAACATCAGAGTCGAAAATCATCATACCGATGTTGGTGGCGTCGGAGGATCGGTGGCAGAAGCGGATTAACTGGCGTCCCAGATGGAACGGCTACTTGAGCTTCAGCATCTGTTTTGTGTTACTAATGAAAATATCCGCCACACGATTTTCAAAATCCACATAACTATCAATAAACTGTATCCAATATGATTCTTGCTACTTCGTTGAGATATTTCTCACATTACCTTTACTATCCTTCCTTTCATCTGTAGAAAAAGAAAGAACTTATGATATCTCCTTGAACCTTCAAAAGGACTCAAATAAAACCTTACCACTTTCGTTGCTCAATTTATTGCATCATCGTGCAACTCAAAAGCTAAGCTTTTTTCATAAGGTTCAATGTAAAACAACTTTGTGATACCAACAGCAACAATATGTCTCGCACAATTATGACGGGAACGTAGTTGTATATAAATTATATCTTCAAAACCACCATTCGAGTCTAGCAGAGATGTAAATGGCATCCATTTCAGCATGAATAGAGCGAGAACTCAATGATTGTTGATACTGGGAGTACCTTCGTAGATTATTCCAGCAACCTTTTCTGCTTGTTCTTGAGTTAACTGAACAGACAACTCCTTAACCTTATCGAAGCAATATTTGCTGTATTTTATCTTTATTTTAATTTTCTTACTTCATTGTAACATTTGCCACACAGCCAGAACACAGCGATGATCATTCACTCCATCATCAACTATAAGGCCACTAGGTAAATTGGAACATCATTGCGTAACAGCAATTAAATTTTCCTTTATATCTCCCTAAAATAGCAGCACCTACGCAATAAAGACATGCAGATTAATAAGAGGCAGAGATTGAGCTGCGTACATACTTGCGCGTTTGGCGTGACCATTAAGCCCATGAATTAGACCAGAAAGCGTTTAACTTTAGTTTAAGGTGAGGCTCTCTGGCCTTGATTATTTTAACAAAATAGTCAGCATCAAGTATAGCTTTTGCTGTTTGCTGTTGTTTAAAGTACCCGGCTTTACTTGGCAGGCACCGCCACTTTGCGCATGGTTCAAAACACGTACCGCCTTTGCCGCGTAAACGCTACCGCGCTGCTGCCATTTTGGTATGCAAAACGGCTGCTGCGGCTGCTGCGTACATACGCTGCGGCACTTTGCTAAGCGCGTAGCGCATAAAAGCCGCAAGCCACGCATACTTTTTTGGCCGCCACCGCAAAACACGCCGTCGCGCACGCCACGCGCCACCGTAGCGTTGCTTTGCTGGGCAGCGCGCGACGCATAAAAGCACGCCGCGGCGCATACGTACGCAACGCCTGCGCTTTTTACTACTCATTTAGGTGTCTTTTCTCCTTTAACCTAGCTGTGCTCATGGCGCCTCTCAATCACCATTTAGAATCTTCAATTATCCTTTTTATATTTCTTCTCTTTTGTATAATAACTCAACTCAAATCAAACGCTCATAGACCCTCTTTCATGAACTACCCCCTCTTTCAAAAGGACAAACTCTATTGGAAATACGATTTAGTGATACTTTGCTCATATTTTCTCCTGCGAAACGCATGATTTTATATTATCTTTAAACAGAGCAATACACAAACAGGCGTAATGCGCGGTTTTTTTTTGCTTAGAACACCGGGCAAAAAGAGTGAAATACCTGAAGTCTATGAAATAAATAAAAAGCTCTTTATGTGCGAGCTTTTGATCTAGTTAAGACATCATTAGCTTAGCACTCTCTCCCTGACTGCTTCATGAACCAGATGAAGCCCAAAACAAATCATGGCGAGAGGCGCCCCATTCAACGTTTCGAGGAAGAAACCCCAAACTCTCTAATAGGTAAATAGCAAAAACAACAAATAACCCCAAAGTATAATATCCTCCTAATCATCATTAGCAGCAAAGATCAGCAGGATACTTTCTGGGACAGTTTCACCTAGCGATAAAACAAACGAGCGGCCGTAGAGGGCTTTCTATGCTGACGAGCAACAGCGGCAGCATGCCTCTGCTGACTAAACTGATATCGACGGTTGTAATAATCATCAATGTGAAGCTAGCAAGGATAAATGGTACGAACATACTCACCATCGCGTGATCTTCATGCTCGAAACAGGAACAAAAGCCTGTTGTTGATGTAGCAATCACCGCATATCATCGTAAAAAGGCAGAATTTCTTCCCAACGCCAAACTCTTTATCGAAGGCGGTTTAAAACACGAACATTGTAGCATAACGAGGGAATGGTTAAAACTCTCATTGAGCACCCTCCGCATACAAAAAAGCAGAATGCACAAGAGACGCGGCACGTTGTGCATAGAAAAGAATCAATGCCTGCAGCACAAAATGCAGTTGGATCATGCTCGATGTGGAACATCTCTCGTTTAAAGCGAAAATTGTCCTTACATATAAAAGTTGGCTGGAAACGGCGTCAATGTGAAGAAAATGTACTTTTATAAATATTGAGTGTAGACATGAACGCGTTTTCTATAATATACAAAGACATGACGTATGTCTTAGCAAATATGCAATATAGCTGCGGTACGGGGACACAGACTTCTTGGCGGTTGCGTAGCTTCAAAGTCCCTTTTGTTTTGTCCCTATAAGGGAACTTTTCGAGTGTGCCATAACCTAGCTAGAGCCTGTTACTCTTCGTTAAAAAAATACAAGAATACCATTTAAGCGCCTAAATAGCTGTGTGAGTTACTAAATACAATGGTAAACCATGAACCATCAAACGGTCGCTTCTTAGCAGCATTCATGTGCTTTTTTTTAGATGTTCTAAACGGTCTCAAACCTGAAAACTTGCATTGGCATACTTATAACGAGCTATTTACCATAACGTCCGTTGCCCGCACCATCTCAGACTCCTCGGCATTTCTGCGTCTCCTGCCCGGCTACATCTTCCCTGTCCATATCGACGCGCCTGATGATTTCTTCGCGTCTGCGCAATATGCACACGAACGCGTCATTTTAACGGCTAAATCGGCGAGCGCCCTGAAAGCCACCACGAACCCCACCGATAACGGTGAAAGGGTGCGCAGTTTACTGTGCGACCGTGAGGCGGCAGACCGTCAGCGGGATTTATTAACTAAGGGGTCGGTTAACTTTGAGATTAATCAGCTTTTTTACTGATGATGAACGGGATTCGGAAGGAATACCCTTGCGGCGCGAAGCGATGTATACATCTAGCATTATATGGTCATTTATTGCAGAAGGGAAGGTAGCATTTTGATAATAGCAAAAAAAACAGCAAAAGGATTAGCAG +>f5c07582-ccd5-4bd8-aa45-4ebb5011fc08_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch101_read12606 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode01/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch101_read12606.fast5 +CTGTTATGCTTCGTTCAGTTACGTATTGCTAAGAAAGTTGTCGGTGTCTTTGTGGTTTTTTTAAATTTTATCGTGAAACGCTTTTCGCGTTTTCATGCGCCGCTTCACTCTGGCATAACGTTGAGGCCGTCAACTAGACGCTCAGTTCTTCCATATGGGGCCAAGTTCCGTCGCCGCGTTCGTATGTTTTCTGTAAGATCAGCAACGCATCTGGCGTGAAATTCACTTTCAGCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGAGAACCAGAAACGATCTCGGCTCTTACCATGTTAAAGCCCCCGAACTGGCGCGCCGTGATATGTTCTCGGCGCGGTTATTTCCGCGCCGTTGATGTTATATCAATACGGTTTGTCGGCCCACTGACACGGCGATCGACACTTACCGACGGTAAAGAAGGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGCAACTACTTACCGTTTATGCGTTAACTCGGTAACCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGATTATGGATGTGGAAAATCTTACCTGTATGCGCGCAAAGCTGTTAAACAACTGGGCATTGCGCCCGACCGGTAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCATTACAATTCATCGCAAATTCACTTCGACTCTGAATCGTCCTGTCCATGTGGCGGGGCAGCGGCAGGTGGATTGCCGTCCGCTGAATAATCCGCAAAAAATTGCGGTTCAATGCTTACCGACAACAACTTGAAAAGGTTTGGTTATTACAACTGGATGACTTCATTATCAAGGACATTATGGGGCTGTTATAACAAAACGCCAAGTCATGGGTGGAACCGCGTAACAGTGGAGTAGGGCACCATCGGCCTGATGGAAATCCCAACAACAGGGCAAAACGCTGGATAGCATTGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAGCAGG diff -r 4c172a4a4c9e -r acd337269171 test-data/reads2.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads2.fasta Thu Jun 20 10:18:48 2019 -0400 @@ -0,0 +1,8 @@ +>92b7f497-20cb-446e-803a-eb3ea8069c12_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read1636 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode02/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read1636.fast5 +TCATTGCTTCGTTCAGTTCTATCGTGCTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTCGTGCGCCGCTTCAAAGCACATTCGTATTTTTTCTATTGACTCACCTTGAGCCATACCCATAGGGACATAATGAATCCTCTCGAAAAACCCACTGGAGAAAAATTTATCGAACTTTTCCCCAATAAAAGTATGGGTAGCTATTTACATAACCATATCACCTTTGTTCAGAAGTTACAACTATACCAATTAGATGGTCCTTGAAGGGATTTGTGAACTTAACGTTCACTCTTTCTGATGAAAAAACAACAATATTAAGCAGCTCTTGGCTATAGTCATCAGGTGACCATTGGACAAGACTATAAACCCTTTAACAAACTGATTGTTTTCATAGCTTTGCTTGCGGCAAATAACTCTTGTATGTTTATCATGCTTGTCATCCTTAGCCCAGACTGCTGTGGTTATAAGGCAATGTTCTTGCTTTGTTGGCGCACTACGTTCAAAAAAATCTTTCAACTTAAGCTTTCTTGAATTAAAAAGATAATCCACCATGCTTCAAGCTCTGCAATTGAAGAAGTTAATATTAAAGAAGCATGGTGGCTAGCTTCTTCTGATGATAAGGCATCATCAGGGGATAGA +>0846a7b6-b5ff-42ef-950f-f344a311c521_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read1965 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode02/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read1965.fast5 +CTTGTACTTCGTTCGGTTACGTATTGCTTCGATTCCGTTTGTAGTCGTCGTGTTTTCGCATTTATCGTGAAACGCTTTTCGCGTTTTCATCGCCTTCAGCAAGGAAGGTAAATCCATACGTCGTCGAAACGCTTTGTGAGTCGGGGCGGCGTCTGAGGAAGTAATCCGTGAGTTCTGGGTTAGGGTTTTGCATATATTTACCGTCCCGCTGCTGGGCGATGGCTCATTGTTATCATTGCCGTATCATCACCTGTTGGTTGTCGCTTCTGAATCAATTTCTGCAGGTGATGCACCCCCAAACGATGGCCGTCGTTGCGGGGGGGTCGCTTTAAAACGACCGCAGAATACTTCAAAACCTTCTACAACGCAAAATAAAGCCCCAGCCTTGGTACGCAAGGCTTGATGCCATTTTTTTATCGCCAGGTTGTCCCTTAAGTATAAATGGGATCGCGCGCGGATCAGACCAACCATTCAACGCCCACCTGCAAAACTGCCACAACGCGAGGCGAAACAGCAACATCAACGCACTATCAACATGATTAGAACTATAAAAACATAACAGCCTCACTCGAGTAGATTGGGTAAATCTATTTAGTCTGTGATTTGCGAACCATACTTTTCGCATCACGACAGAACCAACTCCATTCGTTCTGGTTGTCTCGATATAGGCAATTAAAAACCGTCTTCGGGTGGTTGGCTATATGAATCCAACCACCTCCTTCTTTTCAATCCCAGCAATGTCGTCAATCACTAAATCGGAACCCACTGGCAAGCCAGCGGGACCTGATTTGAGTTTGACGATTTCGGAAAACTTGGCTGGCAGATTTTTTAATCTGACTGCGCAGAAGTGTGAATGATCGGGCGCAGCCCTGACAGATCGTTTTAACGCCGACATCGTGATGGCGGTGTGCCAAAACACATCTTCCAATCCCAACTGAGTTCGGAAACCCGTTCAGTAATTAGTGAGCACGTCACTTTGCGGCGTTAGCCTATCACCATCGGGTAGAACCAATAGGACAAGATGTGTTTTTGGAGTACCGACAACGGCGCCCTCTCAAAAAAATAAGTGGTCGGGGCTGCGCGACACTAATCTGATTCAGCTTTATCTTTTGACGGCGAGCGACGGTACTTTTGATTCGGTTTTCAAAAGCAATGCGTGAGACATCATCATCTCGCTGATATCAATGCAGAATTGCCATATCGGCATCGGTTTCTGATCGAGGTTTCGAGTATGTCTTTATGCGCCACCGGTGACACAAATCCGGCGCTTATGTATATAGCAGTCAATTCGAGACCATTCGTCAGCAACCATGTCAGCCGGCTTCTCCGTGGAATATTTCAGGGTTTCAGCCACTGCGCCCGTACAAGTTCGCAGTTCTGAAGCCAATGATTCTCCGTCATAGGCTTGCGGGGTTTTACTGCTCGAACATCAACATTCGGATCGCCAATTCACCAAACCTCTCTTAAGCTCTGCCCAGCGGTCATGTTTAACGTAATCTCGCGTGAACGTGAGGAGGAACCATCATCAAGTGTGAAAGTGCGGGTGCGCACTACCGTCACTTCCACGTGTGGCTTCCGTGGTGCGGATCAGCCCTGAACAGGTCGAAGCGTTCACTTTGCGATCCTTAAGTCTTTGGAAGATACTGCATTCGGTTAAGTGTCTGCCCATTTCCCCCGATCGCGCGGTTTCGCACTGTGAGGGTCAGGAACATCCGGCGATGCATCTGGATAATCCGCAACAATACGCGGCGAGGACTGATATTGAACTGCCATCGGGATCGCGCTCCGCGGCAGACGGGACGGTGCCGGACGCAGCAGAAATGCGCCTCACGTAGCTAAGGCGGTTTCTCCAGTTTCTTTGGTGAATAGCAAACCTCAGTAAACCGCCACAGGAACTGCCACGCGCCCCA +>c9cf0f87-b530-42eb-baba-d2f7bf184d51_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2166 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode02/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2166.fast5 +TTGGTACTTCGTTCCAGTTACGTATTGCTTCGATTCCGTTTGGTCGTCTGTGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTCGTGCGCCGCTTCATCCTGTCTGCCAATGGAGGCGATCCCCTGATGTGGCAAGCCCGTTTTATCAGTCCTGCTGGACCATTGTTGGATTGTCAGATGCGGATGTTCTGACCCTCCACAGTGCGAAGCTTACCGTCGGGGGAATTGGGTGAACGCAACGAATGAACATGGCCTTCCAGCGACTAGGATCGCAAGTTTCATTCATTTCAGGGCTGGATCCGCACCACGGAAGTGCACGTGGAAGTGACTGGTAGTACCACCCGCACTGCACACTCTGATGTCGTTCCTCCCTCAATGTTCACGCGAGGATTACGTTAAAACGCGTGCGCTGAAGCTTTGGCGAAGGTGTTTGCGTGAGATTACGATCCCGAATGTTGATGTTCGGCGGTAAGCCCGCAAGCCTGGACGGAGAATCATTGGCTTCTGCAACTGCGGAGACTTGTACGGGCAGTGGCTGGCTTCCTGAAATATTCCCACAGCAGCCGGCTGGCATGGTTGCTGATCTGAATGGTTCCTCAATTGACTAAGCGAACATAAGCGCCGTTTGGCGGCTACCAGTGGCACTCAAAGACATACTGAAACTCGATCAGGAAACTGATGCCGATATGGTAATCGAGGGATGACGAAATATTCCCGAAGGCGATGATGGTTCACATTGCTGGTGGAAAACCGAATCCAAAAAGTACCGTCGCTCACCGTCAAAAAATAAAAGCTGAATCAGACTGACGGGGTGTCAGGGGAAGCAGCCCCTGACCACGACGTTTTGAGGAGCGCCGCTTGTCGGCGGTACTCCAAAAAGCTTGCTCACTATTGGACTTTCTGCTCCGGTACGAGATGGGGCTAACGCCTGCAAAGTGACGTCACTCAGGTACTGAGTTCGGTTTTCCGAACTCCAGTTGGAACAGATGTTTTGGTACACCGCCATCACGATGTCGGGAACAATTCGTCAGGCTGCGCCCCGACACCACCATAACACCTCACGCAGTCAGATTAAAATCTGCCAGCGCTCGCCGTTTCGAAATCGTCAAACTCTAAATCGTGGGGTCGCTGGCTTGCCGGTGAACATTAGTGATTGTTGTTTATTACTAGTGAAAAATTGGCTGAGGTGGTTGGATTCACGAAGTGAGGACAACCTGAAGACCAGTTTTAATTGTCTGTGCCGTTAGAACGATGGAGTTGGTTCTGTCGTGATGCGAAAAAAGTATGGGTTCGCAAATCACAGACTAAATAGATTTACTTAATTCCATTCGAGTGAGGGTTGGTATGTTTTTTTTGAGGTTCTTAATCATGTTGATAATTGCGTTGGCGTTGCTGTTTCGCTCACGGCGTTGTGGCAGTTTTTTTTGGTGGCGGGTGGGCGTTGAATGGCTGGTTCGTCCGCGCGATCTTGTATTTATAGCAAGGGATAACTCTGGCGATAAAAAAATAGCGTCAAGCGCACGGCAAAGGCTTCAGGGAACCTATTTGCGTTGTAGAAGGTTTTGGAAGTATTCTGTTGTTGGGAGTAGTGCCTTAAAACGACCCCGCAGCGGTATCGTTCGGGGTGTGACACCTGCAGAGAATGAGAGAGATAGATAGGGAGAGATAGATAGGGAGAGATGGTAGGGAGAGATGGGGAGAGATGGAGAGATGATAGGGAGAGATATTAGAGAGAGAGAGAGAGAGAGAGAGATATTAGAGAGAGAGAGAGAGAGATCT +>debff1cf-2020-4871-972d-56db0aa5318f_Basecall_1D_template DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2209 /backup2/RawData/nanopore/20170426-nano1/Basecall/workspace/barcode02/0/DESKTOP_HT0CTEN_20170425_FNFAE23458_MN19144_sequencing_run_Rap_Bar_nano1_95462_ch100_read2209.fast5 +GAGTACTTCGTTCAGGTCTTGTTGTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGTGCGCCGCTTCAACGGTTTGTGATGGGGTGTCACTATTATTTATGCGTTAGTGAACCAGAAAAAATCCCCCGTTGGCATAGGGGGATGTAGGAAGAAATAACCATAGGTGTTATTGTTTGTATAACGTGAAAAAGACTGCACGCAACTTAAGTCTGCCATTGCCACTTTCCAGTTTTCAACATAAAGGTTTATAACAATGTGGAATGGAGAGTGAGCTTTTAGGTTTCAGCTCTAAAAAATAAATGTTGTGGTGGCTGGTTGTTCTTGTTCTGTATATAAACACTATCGACTATTATTATGACCGGGAACATTCCTTACCCCTCTGCGCTTTCATAAGTTTTTGTCATTAATTAATAATGAACTGAAATGCAGTTCCGAAATACCTCCATTTACTGTGCTCTGACATGGTACCGGAACGCGTGAATTTTTATGTTATAGAGTTTATTTTTAATGTCCAGATGGTTTAATATCCATTTATATGTATTAGCTGTATGGATAGTACTTTGGTTTTATTGTATAACTTGTTTTTTTTTATTGTTTTATTGGTGTGTAATCTTTAAGTTAAAAACGCTTGAATACCGGGGCAAAAGTAGGAAAAGCAAATGGATACTCGGTTGTGTGACTTTTAAACTAATGGAACTATAGAGTGGAGGTTGTTACAGTCGCTTTTATAAAACAGCAACCAGTTTTTTCCAATAGTCTGATGATGGTTGTGACAGTTTTATCGATATTGTTCAAAAATGTTTCTTGTTGAATGTTTTTGGTCTATTGGTGTCTGGTTTTATTTTTCTGCTATTACATATCAAGAAATGTGATTGGTGTAACAGAGTGGAATGCGATGTGGATAAACTAATTGGTTGTCGAATCTAAGAACTTTACTTAGAAAACTACACTTGTTTTTGTGTATGGGTTGTTAGTAATTTGATACTGTCTATGTGTTTTCGTGCATTTATAAATCCTTCGTCAGGATCTTAAATTTTATTGTTGATGTGTAGGGCCTGTACGGTAATTGTAGCTACTTCATCGTTGTTCGAACACAACCAACAACCTGTTGTTTCTCTTGGTTCCCCACTTTTGACGGGGTTTTATCTCTTCCGTGCATCGTAAAAAGCAGGACTCCTTCTAAACTGTTTAAGGGCATCACTAGAGCATTTTGCGTTGTGATATTGAGCATCCGATAACTAAATATTTCCATAGACTCTGATTCTTGCCACCGACCGGGTCGGTTTTGTCTGGCGAGATGTGAAGTTTGGGCTGGAAACCCCGTCAGGCGCACCGGGGGGGGAGAAAACATCGTTTTGAGCCGTTCGGTGTTCCAGAACGCACAGGCTCGATCTTTATCGCTTTCGTGCATGGTTATGCAACGCCCTGAAACGGTCTGACGCGTTTTTCTGGTTTGGCTGGTGTTTTCTTGTCTTTTGCGTTTTTGCGCCAGAAAGCGACTGAGGGCGTTTTAGAGTACAACAGGGTATGGTAAATGGATCGGGTTTTGGGAGTTCGACAGGATTTGCCGTTGGGTGTGATGTAAACGACTTGAAAAAAACAAACGCCCTGAAACCATGTCAGATCAGATTCAACATAAAGCCAGAGGAGCGTGTAGGTCGCATAACCGGAAGATCGTAACACATGAGCACGCCGCCTTCAACCACTTCGACGAAAATTTACTATCTATCTTTACGGATGATCTCGCTTTTGGTCTTCGCATCTCCGGCAAGGGCGTGCTTCTTGCGCAGTACGCTGGCAGAACAGCCTCATGCGCAGTTCTGGCCAGTTTGATTGACCGCGAGGGCTGCGGTGACTGGAGCGACGGAACGCTCCCGCATGAACATCACCGTTAAAAATCTGTGAAACCGGACGCTCACCTGCTCTACGCACTTTAACATCGCCGTGAACCGCGCCTGATGCCTCGGTTAAAGGCGCTGAAATATCTTCCGCGGTTGAACGTTCGCTGTGTGAAACTGCGGATGTGAACTACAGCGGCCTGATTTTGCAAATCCGTTCCACGGAATGGCTGGTGATGGAGCGCGAGGCTATACCCTCGATGAACTGGCTGATATCTCGATTTGAAGAGCGCCTCAGAGCGTCGTAGCATCGATAAACATTACGGGATGAGGGCGAAACTGCCACCTGTTCGAAATGACGCGCAAATGGGCTTACAGGAGCGATTCGTGCATGACCAGCGTTCTCTGATGTGAAGTGATCCAGCGTGTCGAAATGTACAATGCGCTTCCCGTTCCGCTTTCACTGGCTGAATGTCGGGCTATTGGCGAAACATTGCGAAACCACACTGAACTCCACGCCGGAAACTTTCGCTGGTATGTGGCTGATACGCACACGCCAGAAATTCAGGCTGCACGGTCGCAAGGGCGGTTCTAAGTCACGGCGCAGCACAGTAGCTGCGCACGCTGAAACCGTGGGAGGCATTAAGGGGATCGTCGCGCACGGTGGCGTACCAACTGAAAAAACGAATTATATTAAGTGGGCAAACAAGCCTATATCAGATAACCAGCGCCTTTCAGCGTCTTTTGAGCAGGTTTGCACGTTCAGCCTGCAATGGCGGCTTTCCGCGTGATTGTGGTGGTAGTGCTCGCCGCAGTCTCATGACCGAGCGTAGCGACGAATGAAACGAGGAAGCCTGGAATAGATCGGAGGTTACAGATGGAGGCACTTGCGCGTCTGGGTTTTGGGGATGTCTCCCAGATCGTACTTCGCTAAGTGCTGTAATCTGTCTTTTTTTTATGGCATGACTCGCCTGTTTTGTGTCTACCAGTCCTGCTTCGATCAGTAGCCCAGCCGCGAAAGAACGGTGATTGCGATTCCCGTCATTGTTTTTTCCCTCGATTCGTTTTTGGACCGCAACCTGTTTTCGTTCTTTCTCCTGTGCCTGTAAGTCGTTTTATTTGCCGAACGTGGAAGTTTGCGTTCCTGTTCTGTGAGTTACAGCTTCGTTGTCGATCGGTCAATAATCTGTCTCTGTCGTGCGGTCTGTTCTCGAAACTTCCTGATGAATCCAGTACGTTTCTTCAGCCATTCGATGTTGCTGATGCGCTTCGTGATGCTCTGGTGTTTTTTTTGCGGTGTGTTGTGGTCGTGCAGTATCTTTTGGGTCGGCAGGTACGGGGGGTATCTTCCGTGCTAATTCGTAAAATTAGAAGGAAGCGCACTTATATATCACTTCGTCGAAAACAGTGCGCTCCTGCAGAGAAACAAACGGCTTATCACCTCAGCGTTAGAATTTGGTAAGCAGGAGCGGGCGCAAACCACGCCGATTACATCGACGCAAAGAAAGCTTCGCGACCGTCAGGATTTGGAGCCATTCCGCACACGATGCGGGAATGGGCGAGGATAATCCTTCCATTTCACGGCGGCGGTTGGTTGAACGATACTGGTGGTTCGACTAAAGGGCAAGTACGTACCGTGAACTGACACCAGGTGCTTGCCTTGAACTGGTGCAGGATTTTGTACGTCAAGTGGCGAACGGCACACTGAGTTTGCCATTCACAATCCAGCGGCATTGACGGCGAACAGCCTCATGCGCATATCATGATGAGTCAGCGTGTGAATGATGGCGTTGAACGTACGTAGTATTTCGGCGCTATGTACCGGTATCCCGAGCAGGGGAGGTGCGAAAAAAAAGTTCGAAGCCGACTCTCACTCAGCAAAAAGTGGATTGCGGGGTGCGAAAACGCTGGGAGGTGGCGGAACATATGGCTATGGCTGAAAGGGCTTCATTGACTGCCGGACGCTGAAGAACAGAGACGTAGACGACACGTCCCGAAGTGCATCTGGGTTTCAAGCTGCTGACGTGATGATAGCAACGTCATGACATCGTAAAAACGGTCTGAACCGGACTATTCCCGCCATGAACAGGCGCGAACGGTCAGGCATGACATCAGGGAATGGCTGGACACTCCAGTTAACAACATGAACTGGTCAGAAAGGCGCAACATGATGTTGCGCGGGAGCATGGCTTTGACATGAGACAAGTGAACAGACCGAACTGCACCTGAACGGGTTGTCAACCGTCGTGGATTTCTATGGGATTCTGAACAGCCTGAAGAAAAATTACACGCTCCTGTTATCGGTGGAGCATCTGCAACATACCGCAATGGAGCAACAGAAGAAAACTGTTGCTGATTCTCTGGCTGTGGCAAGGAAACTCTTCGCGAGATATCAGGATATTTCTGACGGTGGCAAACAGGTCGGAGTTAAGTCAATGGTTAAGAAGCTGGACGCTTGAGACTGATGGCTTATCAGCAGACCCTCTTGGCCTGGAAGAAGTCAGTCCATATAAACTCGCCTGAACAAGGATATTCTATATCGATACGAATGCTTTCTACTCAAGATGCTAAATAAGCTGGAAATGAGGTAATCAATCGTAGTTATGTTCTATCAGATGGAATAATTTCAAATGTCTCCGTCAATATCAAATAATA