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view test-data/test.sam @ 9:d3a7b17714c1 draft default tip
planemo upload for repository https://github.com/open2c/pairtools commit 13aa776596c927adff29d936740ac80f0e37375d
| author | iuc |
|---|---|
| date | Tue, 21 May 2024 16:05:04 +0000 |
| parents | 68413c5eb035 |
| children |
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@SQ SN:Test_seq LN:450060 @PG ID:bwa-mem2 PN:bwa-mem2 VN:2.2.1 CL:bwa-mem2 mem -5SP -T0 -t 8 test test_hic_reads_1.fq test_hic_reads_2.fq A00126:224:H7HMWDSX3:3:1101:4779:3098 113 Test_seq 166134 43 78M = 214824 48691 GGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFF NM:i:0 MD:Z:78 MC:Z:78M AS:i:78 XS:i:63 XA:Z:Test_seq,+214829,5S73M,2; A00126:224:H7HMWDSX3:3:1101:4779:3098 177 Test_seq 214824 5 78M = 166134 -48691 AAGAGCCCAAGAGACAGAAAGGGCCACATGAACCACAGACCTACATCATCCTGAGACCAGAAGAACTAGTTGGTGCCC FFFF,FFFFFFFFFFFFF,FFFFFFFFFFFF:FF:,FFFFFFF:FF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:3 MD:Z:1T33G33A8 MC:Z:78M AS:i:66 XS:i:63 XA:Z:Test_seq,+166134,78M,3; A00126:224:H7HMWDSX3:3:1101:2908:3239 81 Test_seq 167546 44 151M = 167331 -366 TAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTACAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACAC FF,F,F:FFFFFFFFF,FFF:FFFFFF:FFFF:FFFFFFFFFFF:FF::F,FFFFFFFFF:,F,FFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFF::FFFFFFFFFFFFF,FFFFFFF:FF:F,:FFFFFFFFFF,F,F:FFFFFFFFFF NM:i:3 MD:Z:82G15T30C21 MC:Z:151M AS:i:136 XS:i:114 XA:Z:Test_seq,+213342,118M2I23M2I6M,8; A00126:224:H7HMWDSX3:3:1101:2908:3239 161 Test_seq 167331 23 151M = 167546 366 TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFF:FFFFF:FFFF:FFFFFFFFFFFFFFF:FF,,FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFF:FF,FF NM:i:1 MD:Z:37A113 MC:Z:151M AS:i:146 XS:i:135 XA:Z:Test_seq,-213553,151M,4; A00126:224:H7HMWDSX3:3:1101:27208:4492 97 Test_seq 194620 60 151M = 194778 290 GCTGTGCAAACCACTGCTCCAGGCTATCTCAGCACTGAGCCAGAGACAAGGGCCAGTTCAGTCACCTGGAAAATCAGGCCAGCATCTCTGAAAAATAGGTGCCTCAGTGTTCACAGAAAAGGGCCATGTATTGAAGTAGGTCAGAGGAAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,FF:::FFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:132M19S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:27208:4492 145 Test_seq 194778 60 132M19S = 194620 -290 CACTCACCAGATTCAGCCCAGGACCAGAGGCTGCCGAGGGCAGGTGTGGGACGAAGTCAAGATGAAGTCAGTTCATTGTGTGGTTTTCGGTACCAGGCTAGACCTAGATTTGAACCCCAAGTCTGCTACTTAGATCATTCATTCAATGTGT FFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:132 MC:Z:151M AS:i:132 XS:i:0 A00126:224:H7HMWDSX3:3:1101:9344:5306 97 Test_seq 449503 7 60M = 449503 60 GTTCGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTATGAGTCAGTGT ,FFFF,FFF:FF,FF:FF:FF:F:,F::,FF:,FFFFFFFF::FF,,:FFF,FFF::,F, NM:i:4 MD:Z:3T44G2C5G2 MC:Z:60M AS:i:44 XS:i:40 XA:Z:Test_seq,+16926,60M,5; A00126:224:H7HMWDSX3:3:1101:9344:5306 145 Test_seq 449503 12 60M = 449503 -60 GTTTGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTGTGCGCCAGGGT FFFFFFFFF:F::FF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:F:FFFF NM:i:1 MD:Z:53T6 MC:Z:60M AS:i:55 XS:i:50 XA:Z:Test_seq,-16926,60M,2; A00126:224:H7HMWDSX3:3:1101:17607:5212 81 Test_seq 83397 60 122S29M = 93852 10428 GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATCAATCCAGCTGATTTTTGTGTGTTTACCTT :FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:29 MC:Z:151M AS:i:29 XS:i:0 SA:Z:Test_seq,94155,-,122M29S,60,0; A00126:224:H7HMWDSX3:3:1101:17607:5212 2129 Test_seq 94155 60 122M29H = 93852 -425 GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATC :FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:122 MC:Z:151M AS:i:122 XS:i:0 SA:Z:Test_seq,83397,-,122S29M,60,0; A00126:224:H7HMWDSX3:3:1101:17607:5212 161 Test_seq 93852 60 151M = 83397 -10428 AAACATGGAACGGCAAAAAATGTGGCACCGACCCGGCTATATGAAGGACACTTGTTTACAGTGTGAGAGCTGAAACAAGAAGGCAGAGTGCGGCTTTGTTTGACCTCTGTTGGAAACGCGTGCATCGGGTGATTCAAAATTTCCACCACTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:122S29M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:17942:7263 97 Test_seq 168528 60 151M = 168717 340 ATTTAGAGTGATGTTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTTTATCATAAATGGGTGCTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATG FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFF:FFFF,FFFFFFFFF:FFFFF,::FFFF::F:FFF NM:i:5 MD:Z:12A68A23T2A26T15 MC:Z:151M AS:i:126 XS:i:91 A00126:224:H7HMWDSX3:3:1101:17942:7263 145 Test_seq 168717 60 151M = 168528 -340 ATTAATGGTTTTTCTGATATTAAACCAACCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGT FFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:27G123 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1101:5683:8108 65 Test_seq 137032 0 28M123S = 170834 33803 GATTTTTATTTTCTTTATGTTGAGGTGTGATCGATCCAAAAAGAAAAACACCAAGACATATTATCATCAAACTTGCCAAAACCAAAGACAAACAGAAAATTTTAAAAGCAGCCAGGGAGAAAAGAAAGGTTTCCTTCAAGGGAGAATCAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF:FFFFF NM:i:1 MD:Z:7G20 MC:Z:151M AS:i:23 XS:i:23 SA:Z:Test_seq,171170,-,119M32S,60,0; XA:Z:Test_seq,+333108,28M123S,1;Test_seq,-298750,123S26M2S,1;Test_seq,-279319,123S28M,2; A00126:224:H7HMWDSX3:3:1101:5683:8108 2129 Test_seq 171170 60 119M32H = 170834 -455 ATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATC FFFFF:FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFF NM:i:0 MD:Z:119 MC:Z:151M AS:i:119 XS:i:0 SA:Z:Test_seq,137032,+,28M123S,0,1; A00126:224:H7HMWDSX3:3:1101:5683:8108 129 Test_seq 170834 60 151M = 137032 -33803 ATCTTACTTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTAAGCATTTCTTGCAGTCTTGGTTTAG FFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:28M123S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:3947:9361 97 Test_seq 171174 60 151M = 171342 297 ATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGA FFFFFFFFFFFFFFFF,,:FF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFF:F:FFFFFFFF::FFFFFF:FFFFFFFF:FFFFF,FFFFFFFFFF,FFFFFFF:FFF,F NM:i:0 MD:Z:151 MC:Z:129M22S AS:i:151 XS:i:37 A00126:224:H7HMWDSX3:3:1101:3947:9361 145 Test_seq 171342 60 129M22S = 171174 -297 TGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTATAAAAATAAAATGCTTGCTTAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFF NM:i:2 MD:Z:58G15T54 MC:Z:151M AS:i:119 XS:i:0 A00126:224:H7HMWDSX3:3:1101:6596:9721 97 Test_seq 213805 11 55M = 213805 55 CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGGAAATGCTCTCGA FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFF:FF:F:FFFF:FF: NM:i:1 MD:Z:42A12 MC:Z:55M AS:i:50 XS:i:45 XA:Z:Test_seq,+41478,55M,2;Test_seq,-167175,55M,3; A00126:224:H7HMWDSX3:3:1101:6596:9721 145 Test_seq 213805 16 55M = 213805 -55 CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCCCGA FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF NM:i:1 MD:Z:51T3 MC:Z:55M AS:i:51 XS:i:45 XA:Z:Test_seq,+167175,55M,3; A00126:224:H7HMWDSX3:3:1101:1777:10175 81 Test_seq 62875 60 126M25S = 62699 -302 TTGTGCTAAGAAAATAATTGTCATTGACCTTAACGAAACCCCTTATTATTTCCAAAACAAAAGCATAATATTGAACCAGCTCTTTTGAAGAGATTTAAATACACTGCATCATTGGCAAAGGGGACTTAATAGTTTTGTGTGTAGGTCTCCT FF,F,,FFF,FFFFFF:FFF::FF::F,F:FFFFFFFF,,,,:F,FFFFFF,:FFF,,FFFF:F,FFF::,,:FF,FF:F:FFFF:FFF,,F:FF:FFFFF:FFFFFFF::FF:,:FFFF:,FF:FFFF,F,,FFFFFFFFFFF,FFFFFF NM:i:4 MD:Z:44C11C7T10G50 MC:Z:151M AS:i:106 XS:i:0 A00126:224:H7HMWDSX3:3:1101:1777:10175 161 Test_seq 62699 60 151M = 62875 302 CGGGACAAAGACATCATCGGAGGTCTGCAATGCCGACTGGATGGCCTCGCTTCCCTCTCACCTCCACAACGTCCCCCTTTCCAATCTGGCAATACCAGGTATTCTTCTCTTCCTCCTTGTTCCTACTGTTTAGTTCTGCCATCTTAGCGTT FFFFF,FFFF:FFFFFF,FFFFFFF,FFFFFFFFF:FFFFFFF,:FFFFFFF,F:FFFFFFF::FF:FFF:FF,FFFF:FF:FFF::FFFFFFF,:F:FFFFFFFFF:FFFFF:F,FFFFF,,FF:FFFFFF,:FF:F,:FFFF:FFF,,F NM:i:0 MD:Z:151 MC:Z:126M25S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:15845:10269 81 Test_seq 168129 0 151M = 167871 -409 TACATCCTTGGTAAGATTTATTCCTAAGTATTTTATCTTCTTGGGGGCTACTGGGAATGCTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTCTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTACGTTTATTTTATAACCTGAGACT :FFFFFF:FFFFFF:FF:FFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF::FFFFFFFF:FF NM:i:8 MD:Z:3C5C30C12T5G33T34T13T8 MC:Z:151M AS:i:112 XS:i:111 XA:Z:Test_seq,+212759,151M,8; A00126:224:H7HMWDSX3:3:1101:15845:10269 161 Test_seq 167871 60 151M = 168129 409 CTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF: NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:18412:10802 65 Test_seq 213099 27 9S142M = 167532 -45568 AAGGTGAGAGAGAAACAAATCACCAACTTCATATGGAAGGGAAAGAAGCTTCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTCTTATACAGCCACAGTAGTCAAAACAGCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:9G30C9T61A9T19 MC:Z:151M AS:i:117 XS:i:103 XA:Z:Test_seq,-167798,151M,10; A00126:224:H7HMWDSX3:3:1101:18412:10802 129 Test_seq 167532 52 151M = 213099 45568 GATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCC FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFFFFFF:FFFFFFFF:F:::F:FFF:,F:F:::,, NM:i:1 MD:Z:144A6 MC:Z:9S142M AS:i:146 XS:i:121 XA:Z:Test_seq,-213356,104M2I23M2I20M,6; A00126:224:H7HMWDSX3:3:1101:18421:11287 97 Test_seq 214825 31 52M = 214829 52 TGAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTG FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:52 MC:Z:4S48M AS:i:52 XS:i:43 XA:Z:Test_seq,-166159,48M4S,1; A00126:224:H7HMWDSX3:3:1101:18421:11287 145 Test_seq 214829 3 4S48M = 214825 -52 AGAACCCAAGAGACAGAGAGGGCCACATGAACCAGAGACCTACATCATCCTG ,FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:13A34 MC:Z:52M AS:i:43 XS:i:42 XA:Z:Test_seq,+166159,52M,2; A00126:224:H7HMWDSX3:3:1101:18801:13416 97 Test_seq 63815 60 151M = 64115 451 TTTTGGAGATCTTCCAGGTGCATTGTCCCCATGGGCCCTGGTTGCTTTTAAGCAAGGAAAGGGCTGCTGAATTTCTTTATGGTTATGTCTACTTGTGTTTTATTAGCTGTTGGCTACAGCTTGCTGGGTAATGCCCGATGGCTATGCTTCA FFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFF:FF:FFFFFFFFFFF:FF,:F:,FFFFF:F:FFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFFFFFFF,FFF NM:i:1 MD:Z:87A63 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1101:18801:13416 145 Test_seq 64115 60 151M = 63815 -451 AGAATGTCATCTTAGGCAGGCCCTATATGGCTCTCTCCTGGGCATGTTCAGTGTAGGTTGGGGCTTCCTCTTTTCTTTCTGTGCTTTGGACGGCTTAGACTGTAACAGCAGCTCTGCCAGTGGCTTGGCTGTGTATTCTGACTCTGTGACT :FFFFFFFFFFFFFF,FFF:FFF,FF:FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFF:FFFFFFFF,FFF:F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:27263:14137 81 Test_seq 213158 9 98M = 213158 -98 ACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACA FFFFFFFFFFFFFFFFFF,FFFF:FF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF NM:i:1 MD:Z:63T34 MC:Z:98M AS:i:93 XS:i:88 XA:Z:Test_seq,+167783,98M,2;Test_seq,+152052,98M,5; A00126:224:H7HMWDSX3:3:1101:27263:14137 161 Test_seq 213158 9 98M = 213158 98 ACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:1 MD:Z:63T34 MC:Z:98M AS:i:93 XS:i:88 XA:Z:Test_seq,-167783,98M,2;Test_seq,-152052,98M,5; A00126:224:H7HMWDSX3:3:1101:20645:14137 81 Test_seq 170274 60 151M = 170038 -387 TATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATTGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGC FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:1 MD:Z:83C67 MC:Z:151M AS:i:146 XS:i:80 A00126:224:H7HMWDSX3:3:1101:20645:14137 161 Test_seq 170038 60 151M = 170274 387 ATTGTGGAGGTGTCTATCTCGCTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTGAGTGCGTAAACATTTAATATGGTTATATCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF NM:i:5 MD:Z:1A7A59C2G9T68 MC:Z:151M AS:i:129 XS:i:81 A00126:224:H7HMWDSX3:3:1101:6054:16297 97 Test_seq 169279 60 151M = 169423 295 CTTATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFFFFFFFF,,FFF NM:i:1 MD:Z:30T120 MC:Z:151M AS:i:146 XS:i:103 A00126:224:H7HMWDSX3:3:1101:6054:16297 145 Test_seq 169423 32 151M = 169279 -295 TCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTAGGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGACCTC FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:44G36C0A13C0C36C11G4 MC:Z:151M AS:i:116 XS:i:98 XA:Z:Test_seq,+39146,151M,11; A00126:224:H7HMWDSX3:3:1101:11225:16767 113 Test_seq 40986 40 58M = 167664 126679 GCAGCTGATATTTGACAAAGGCCCAGTGTCAGTTAATTGGGGAAAAGATAGTCTTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:21A23T12 MC:Z:58M AS:i:48 XS:i:34 A00126:224:H7HMWDSX3:3:1101:11225:16767 177 Test_seq 167664 0 58M = 40986 -126679 AAAAAGACTATTATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF NM:i:1 MD:Z:11C46 MC:Z:58M AS:i:53 XS:i:53 XA:Z:Test_seq,+213317,58M,1;Test_seq,+40986,58M,2; A00126:224:H7HMWDSX3:3:1101:6352:16814 97 Test_seq 118685 60 151M = 118910 376 CCAGTGATACAATGAAAGATATTTGTTAAAGATAATTTTCACCTTAAAATAGTTTTAATTGGAATGTTTTTCTTATTGTAGTTAGAAATGTGTCTATTGTGACCAGTCATACTTTATGACAATAAGGTAATCTCAGTGTGTAGGAGGATCG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFF:FFFFFFF NM:i:2 MD:Z:139C10C0 MC:Z:151M AS:i:145 XS:i:0 A00126:224:H7HMWDSX3:3:1101:6352:16814 145 Test_seq 118910 60 151M = 118685 -376 GATATGAGTTCAGAAAACCAAACCAAAACCAAACCCATTGCCGTCAAGTCGATCCCGACTTGTGGAGACTGTATAGGACAGAGTAGAACTGCCCCAGAAGGTTCCCAGTTTTTATGGGAGCATATTGCCACATCTTCTCCCAAAGAGTGGC :FFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:25 A00126:224:H7HMWDSX3:3:1101:3088:17143 81 Test_seq 218307 60 133M18S = 218095 -345 ACTCTATTGCAAAGCACTGTTCTTCCAGCTCTACTGCCATCTCATGTCAGCCTTCCAACAACCTTATAAGGTATATACTATCATTGTTAACATCATTTTCATTTTACGGATGAAACTGAGGAGTAGAGAGATCTAACTTTTTATTTCAAAG FFF:FF:FFFFFFFFFFF:::FF:FFFFFFFFFFFFFFF,FF,:FFFFFFF:F,FFF,,F,,FFFFF,,::FF,FFF:FF:FFFFFFFFFFFFFFF,FFF:FFFFFFF:FFFFFFFFFF:,FFFFFFFF:FFF:FFF::FFFFFFF,,:F: NM:i:1 MD:Z:73G59 MC:Z:151M AS:i:128 XS:i:0 A00126:224:H7HMWDSX3:3:1101:3088:17143 161 Test_seq 218095 60 151M = 218307 345 ACCAGTTGCTGCCAAGTCATTTCCATCTCATGGCAACCCCAGTTATGTCAGATTAGAACCTCATACCATAGGAATTTTAATGGCTTGTGACCTTTCAGAAATAGATCACCAGGTCTTTCTTCTAAGGTACGTCTGTGTGGACTCGAACTGC F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFF:FFFFFFFF:FFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFF,FFFF:,FFFFFFFFFFFFFFFFFFFFF,:F NM:i:0 MD:Z:151 MC:Z:133M18S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:9760:17957 81 Test_seq 113172 60 133M18S = 112944 -361 ATGGGTTTTCAAAAGGGGCTGAGAATCAGGGAATCAGGAAGGACTTCATAGGGAAGGTGATATTTAATATGGGTTATGAGGGCTGGGACAAAGATGTAAACAGGGTGAGAGAATGTCTCACAGGAAGGTGTTAGATCTTGGCTGAAAGCAA FFFFFFFF:FFFF,F::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:0 MD:Z:133 MC:Z:151M AS:i:133 XS:i:0 A00126:224:H7HMWDSX3:3:1101:9760:17957 161 Test_seq 112944 60 151M = 113172 361 GAACTGCTCACCTTTTGGCTAGCAGCCAAGTTCTTAACTACCACGCCACAAGGGCTCCTTCATGCCAAGGTAGTGCACCTTAAAAGAAAAAAGGATCTGTGTCCTCAAGAAACCTGTGGTCTCTAGCGGTAAAAGAAAGGAAGAGAAAATA F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFF,FFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFF:FFFF NM:i:0 MD:Z:151 MC:Z:133M18S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:16342:18458 81 Test_seq 214212 34 151M = 214062 -301 CAATGGATGAATGTTTAAATAAATAGTGGTATATTCACACAATGGAATACTACGCATCGATAAAGAACAGTGACAAATCTGTGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATTGGTCAGAGGCAAAAGGA ::F:FFFFFFF,F,:::FFFF:FFFFF,F:FFFFFFF,::FFF,FF:F:FFF:FFFFFFFFFFFFF:FFFFF,,FFFFF:FF,FFF,FFF:F,F:F:FFFF:FF:FFFF:FFF,,F:FFFF,FF:FF,::FFFFF:,FFF,F:FFFFFFFF NM:i:7 MD:Z:13G10T27T5A15G52T6A16 MC:Z:151M AS:i:116 XS:i:97 XA:Z:Test_seq,+166672,151M,11; A00126:224:H7HMWDSX3:3:1101:16342:18458 161 Test_seq 214062 60 151M = 214212 301 AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGATGGAAGCAACCAAGGTGTCCATC ::FF:FFFFFFFFFFFFFFF:FFF::FFF:FF,FFFFFF,::FFFFF:FFFFFF,FFFF,F:F,FFFFFF,F:F,F:,F:FFFFFFF::FF,FFF,FFFF:FF:F:FFFFFFFFFFFFFFFF:F:,F,:FF,FF:F:F::FFFFF::,FFF NM:i:1 MD:Z:127C23 MC:Z:151M AS:i:146 XS:i:108 A00126:224:H7HMWDSX3:3:1101:25048:19601 97 Test_seq 374646 60 151M = 374891 372 CCTCACTACTCAGCCTTCTCTGGCACCACCCTGGTGTGTGTGGTGGGTAGCAGGGGTTGAAACTCCTTGTTATAGTCTGCTGAGGATGGATGTCTAGGCTCTCCTGTGAGCCTTTGTAGTCGACAGTAGAGATGGGACCACAGTGTTTTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF NM:i:0 MD:Z:151 MC:Z:127M24S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:25048:19601 145 Test_seq 374891 60 127M24S = 374646 -372 ACTTCTTTTTTTTTGGTATGTTCTTTCTTTTACATTTCAGAGTTGCTGGCTTCTTCAGCTCCAAGTCCGGGATATATGAGGCTAAAAGAAAGCCCGGGGAACTCACCACTGTTTTTTTTCCTTGAGTGATCCATAGGGTTTTCGCTGGCTG FFFFFFFFFFFFFFFFFFFFF:FFFFFF,FF,FFFFFFFFFF,FFFFFFFFFFFFF:FF:FFFFFFFFFFFFFF:FFFFF:F:FF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF NM:i:0 MD:Z:127 MC:Z:151M AS:i:127 XS:i:0 A00126:224:H7HMWDSX3:3:1101:19289:20901 81 Test_seq 168616 60 151M = 168345 -422 TTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATC FFF:FFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:100T50 MC:Z:151M AS:i:146 XS:i:71 A00126:224:H7HMWDSX3:3:1101:19289:20901 161 Test_seq 168345 60 151M = 168616 422 GATCACGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGAATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:1G3T14A28T1G98T0 MC:Z:151M AS:i:129 XS:i:71 A00126:224:H7HMWDSX3:3:1101:9218:20870 97 Test_seq 169231 60 151M = 169310 230 ATTCTTTTAATTTCATTTGGCTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTTGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::,:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFF:FFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFF,F:,,FFFFFFFFF:F:FFF NM:i:2 MD:Z:37T16C96 MC:Z:151M AS:i:141 XS:i:72 A00126:224:H7HMWDSX3:3:1101:9218:20870 145 Test_seq 169310 60 151M = 169231 -230 TTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCC F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:110 A00126:224:H7HMWDSX3:3:1101:12933:23672 81 Test_seq 168554 60 151M = 168347 -358 TTGAATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:3C147 MC:Z:21S130M AS:i:147 XS:i:82 A00126:224:H7HMWDSX3:3:1101:12933:23672 161 Test_seq 168347 60 21S130M = 168554 358 ATACCCATCTGCACAGATTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGTGGTGATAAAGG FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFF:FFFFFFFFFFFFFFF NM:i:3 MD:Z:18A28T70C11 MC:Z:151M AS:i:115 XS:i:67 A00126:224:H7HMWDSX3:3:1101:4869:24142 81 Test_seq 170681 60 151M = 170390 -442 CGTTCTATATCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTTTTGTGATCGTTTATCTATTGATTTCCATGATTTCCTGTTATGTGTTTTATTTTGTCTATTTATTTATTTTTTGGAAT FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:7 MD:Z:4G6A38G22A45A9T16A4 MC:Z:151M AS:i:116 XS:i:0 A00126:224:H7HMWDSX3:3:1101:4869:24142 161 Test_seq 170390 60 151M = 170681 442 TCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFF:F,F::F:FFFF NM:i:1 MD:Z:100T50 MC:Z:151M AS:i:146 XS:i:34 A00126:224:H7HMWDSX3:3:1101:30590:25285 97 Test_seq 168735 60 151M = 168990 370 ATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:F:FF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFF,:FFFFFFFF:FF,FFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:115M36S AS:i:151 XS:i:85 A00126:224:H7HMWDSX3:3:1101:30590:25285 145 Test_seq 168990 60 115M36S = 168735 -370 TGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCGGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGAGTGATCCCTTCCCTATTTTGCTCTGCTAAAACCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:57A57 MC:Z:151M AS:i:110 XS:i:51 A00126:224:H7HMWDSX3:3:1101:21441:26631 97 Test_seq 264606 60 151M = 264850 360 TGCCACTGGTTAGCATCTGAACTCTTAACCACTGTGCCACCAGGGCTTCTTCCATGTGTCACCAGAGCGAGCTTCTTCCAGGACTACCCTAATATAAAGACTGGTTCGTAATAGACGTTCAGTGAAGACTGAGTGGGTGATGGCTGAATGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:116M35S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:21441:26631 145 Test_seq 264850 60 116M35S = 264606 -360 TGTGTGGTTATCCTCCATTTTGAGAATTTCCTATGTGTTATAAATCATAATCTCTGCCTGTGGTTAAAAGGATTAGGGTGGGACATCATACTCTTGCTCAGGCCGCATCCCTGATCGATCGATAGAGTTTGGATTTTCCTGTAAGAGGTTA FFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFFFFFFFFF NM:i:0 MD:Z:116 MC:Z:151M AS:i:116 XS:i:58 A00126:224:H7HMWDSX3:3:1101:23999:27367 113 Test_seq 167167 52 151M = 213895 46729 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:76T74 MC:Z:151M AS:i:146 XS:i:121 XA:Z:Test_seq,+213717,151M,6; A00126:224:H7HMWDSX3:3:1101:23999:27367 177 Test_seq 213895 22 151M = 167167 -46729 GATGAGATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACACAAAATAATAACTGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATGTATCTGGCGT F:FFFFF:,FF,FFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFF:FFFF:,FFF:FFF,:FFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF NM:i:7 MD:Z:18T3A37A22C9T46C7T2 MC:Z:151M AS:i:118 XS:i:106 XA:Z:Test_seq,+166989,151M,9; A00126:224:H7HMWDSX3:3:1101:31458:29951 113 Test_seq 167916 60 151M = 213171 45256 TATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATATATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCAC F:FF::FF,FFFFFF:FF::FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:::FFFFFFF:FFF:FF,FF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:1 MD:Z:73G77 MC:Z:151M AS:i:146 XS:i:58 A00126:224:H7HMWDSX3:3:1101:31458:29951 177 Test_seq 213171 7 151M = 167916 -45256 AAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAAGAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGC ,FF::,FFFFFFFFFFFFFFFFF,F:FFFF:FFFFFFFFFFFF,F,FFFF,FFFFFFFFF,::FFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFF,F:FFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF NM:i:2 MD:Z:83C57A9 MC:Z:151M AS:i:141 XS:i:136 XA:Z:Test_seq,+167717,151M,3; A00126:224:H7HMWDSX3:3:1101:22001:30201 97 Test_seq 366564 18 22M129S = 369501 3088 AGGATTTCATTTTATTTGGATCTAAAAAGAAAAGGGTGGTAGTGGGGGGAAACCTAAAACAAATCCTACTGCTGTTAAGATTATTATTAGGTTACCTTACCATAAGTGACTGTAGACTATTGTGGAAGGAATCCTGGTGTCACAGTGGTTA FF,F:FFFFF:,:FF:FFFF,,:FF:FFF:FF:F,:FF,,F::,,:,:,FFF,,FFFFF,FFFF,FFFF,,,F,:,FFFFFF:,,FFFFF,FFF:FF:,:,FFFFF,,FF,,FF:FF,FFF,F,,FFFFFFF::,:,::,:FFF:F:,:,F NM:i:0 MD:Z:22 MC:Z:151M AS:i:22 XS:i:0 SA:Z:Test_seq,369346,+,22S129M,60,2; A00126:224:H7HMWDSX3:3:1101:22001:30201 2145 Test_seq 369346 60 22H129M = 369501 306 TAAAAAGAAAAGGGTGGTAGTGGGGGGAAACCTAAAACAAATCCTACTGCTGTTAAGATTATTATTAGGTTACCTTACCATAAGTGACTGTAGACTATTGTGGAAGGAATCCTGGTGTCACAGTGGTTA :FF:FFF:FF:F,:FF,,F::,,:,:,FFF,,FFFFF,FFFF,FFFF,,,F,:,FFFFFF:,,FFFFF,FFF:FF:,:,FFFFF,,FF,,FF:FF,FFF,F,,FFFFFFF::,:,::,:FFF:F:,:,F NM:i:2 MD:Z:76T40G11 MC:Z:151M AS:i:119 XS:i:0 SA:Z:Test_seq,366564,+,22M129S,18,0; A00126:224:H7HMWDSX3:3:1101:22001:30201 145 Test_seq 369501 60 151M = 366564 -3088 AGCAGTTCGAACCCACCAGTGGCTTTGTGGGAGAAAAGACCTGACAATTTGCTTCTGTAAAGATTGTTACTCTTGTTAGGTGCCGTCAAGTCTGTTCCAACTCACAGCCACCCTATGTACAATAGAAGGAAACACAGCCCAGTCCTGTGCC FFF:FFFF,FFF,F,,,,FFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFF,FFFF,FFF,FFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF::FFFFF:FF:FF:FFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:22M129S AS:i:151 XS:i:19 A00126:224:H7HMWDSX3:3:1101:28736:30655 97 Test_seq 169255 60 151M = 169334 230 GTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTGTTTCTTTAGTCGGTCTGGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGT FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFF,FFF NM:i:4 MD:Z:13T40T11A4A79 MC:Z:151M AS:i:131 XS:i:88 A00126:224:H7HMWDSX3:3:1101:28736:30655 145 Test_seq 169334 60 151M = 169255 -230 GTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTGGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACT FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:110T40 MC:Z:151M AS:i:146 XS:i:103 A00126:224:H7HMWDSX3:3:1101:13774:31015 97 Test_seq 170280 60 151M = 170582 441 TTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATT FFFFFFFFFF,FFFFF:FFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:F:FFFFFFFFF:FFFF:FFFFFFFFFFFFF,FFFFF, NM:i:1 MD:Z:96C54 MC:Z:16M12I123M AS:i:146 XS:i:83 A00126:224:H7HMWDSX3:3:1101:13774:31015 145 Test_seq 170582 60 16M12I123M = 170280 -441 TGAGTCGTTACGTTTTTCTTGGTTTTTGTCTTGAGTTATAGAGTTGTTATACCTTTTTGTGGTTACCTCATTATATACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATTGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTC FFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FF NM:i:22 MD:Z:27G2A3A3T17T5T36C3G6A18C9 MC:Z:151M AS:i:73 XS:i:19 A00126:224:H7HMWDSX3:3:1101:31629:31062 65 Test_seq 365751 60 151M = 372668 6918 CACACTCATAAAACAAGAAAAACACATGAGTTGTTTTTAAAGCCAATGAAATGCAGAGATTATCAAACAATATCGTTAATATGACACGCAAGTAAAATTATACTGAAGATCACTCAAAAACGGTTGCAGCAACTGCCAGAAATTCAGGCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF:F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FF,FFFFF NM:i:0 MD:Z:151 MC:Z:24M127S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:31629:31062 129 Test_seq 372668 30 24M127S = 365751 -6918 TCATCCAGGTTGTTGCATGTTTTAGATCCATCTGACATCAGCAATGATATCCCTGGTTCCACATCCTCTTCTGAAACAGGCCTGAATTTCTGGCAGTTGCTGCAACCGTTTTTGAGTGATCTTCAGTATAATTTTACTTGCGTGTCATATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFF,FF NM:i:0 MD:Z:24 MC:Z:151M AS:i:24 XS:i:0 SA:Z:Test_seq,365828,-,127M24S,60,0; A00126:224:H7HMWDSX3:3:1101:31629:31062 2193 Test_seq 365828 60 127M24H = 365751 -204 AATATGACACGCAAGTAAAATTATACTGAAGATCACTCAAAAACGGTTGCAGCAACTGCCAGAAATTCAGGCCTGTTTCAGAAGAGGATGTGGAACCAGGGATATCATTGCTGATGTCAGATGGATC FF,FFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:127 MC:Z:151M AS:i:127 XS:i:54 SA:Z:Test_seq,372668,+,24M127S,30,0; A00126:224:H7HMWDSX3:3:1101:32398:33238 81 Test_seq 449330 0 59M = 383985 -65404 TCCTGGCTGAAAGCAGAGAATACCAGAAAGATGTTTACCTGTGTTTTATTGACTATGCA F,F:FFFFFFFFFF:FFFF,:FFFFFF,FFFFFF:FFFFFFFFFFFFF::FFFF:FFFF NM:i:1 MD:Z:18C40 MC:Z:59M AS:i:54 XS:i:54 A00126:224:H7HMWDSX3:3:1101:32398:33238 161 Test_seq 383985 0 59M = 449330 65404 TTCTGGCTGAAAGCAGAGAATACCAGAAAGATGTGTACCTGTGTTGTCATGACTATGCA FFFFFFFFFFFFFFFFFFF:FFFFF,F::FFFF::FFFFFFFFF:,F,,FFFFFFFFF, NM:i:6 MD:Z:1C26G5T10T1A0T10 MC:Z:59M AS:i:33 XS:i:33 XA:Z:Test_seq,-333519,59M,6;Test_seq,-116683,56M3S,5;Test_seq,-316244,10M2I47M,7; A00126:224:H7HMWDSX3:3:1101:18602:33395 97 Test_seq 171431 60 151M = 171715 435 CACAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTAAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFF:FF::FFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:1G56T35A22A33 MC:Z:151M AS:i:134 XS:i:47 A00126:224:H7HMWDSX3:3:1101:18602:33395 145 Test_seq 171715 60 151M = 171431 -435 TTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGAT F::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:2T148 MC:Z:151M AS:i:148 XS:i:0 A00126:224:H7HMWDSX3:3:1101:13042:33630 97 Test_seq 166363 60 151M = 166544 325 TTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF,F:FFF:FF:,FFF,FFFF:F,:FFFFFFFF::FF:F, NM:i:0 MD:Z:151 MC:Z:5M3I18M4I121M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1101:13042:33630 145 Test_seq 166544 60 5M3I18M4I121M = 166363 -325 CTGATTGATGATCAATAAAAAACCCTCTCCCTCCCTCCCTCCCTCCCTCCCCCCTTTGTAACCACATAAGTATGTGTTCTTCTCCGTTTTTTCTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCTTTTGCCTCTGAC F:,FFFFFFFFFFFFF:FFFFFF:F,FFFFFFFF:FF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFFF:F:FFFFFF:,FFFFF,FFFFF NM:i:9 MD:Z:59A34C49 MC:Z:151M AS:i:119 XS:i:62 A00126:224:H7HMWDSX3:3:1101:29966:36072 81 Test_seq 170207 60 151M = 170104 -254 TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC FFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:1 MD:Z:55A95 MC:Z:151M AS:i:146 XS:i:88 A00126:224:H7HMWDSX3:3:1101:29966:36072 161 Test_seq 170104 60 151M = 170207 254 TCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTT FFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFF:F:FFFFFFFFFFFFFF:,:FFFF:F:FFFFFFFFFF NM:i:6 MD:Z:3C7G20A32A25C0A58 MC:Z:151M AS:i:122 XS:i:49 A00126:224:H7HMWDSX3:3:1101:3595:36620 81 Test_seq 167826 60 151M = 167567 -410 ATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCACATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGA FFFFFFFFFFFFF::FFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF::F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF,FFFFFFF:FF,FF:,FFFFFFFFFFFFFFF NM:i:1 MD:Z:90T60 MC:Z:15S136M AS:i:146 XS:i:87 A00126:224:H7HMWDSX3:3:1101:3595:36620 161 Test_seq 167567 30 15S136M = 167826 410 ACCAACCAGCTAGACTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATTGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGATAAAAAGACTATCATTTCCCCAAATGACTGACACTGGGCC FFFFFFFF,,FF:FFFFFF,FF:FFFFF:FFFFF:FFFFF:FFFFFFFFFF:FFFF,FF:,,FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:,:F,FFFF:FFFFFFF:F:FFFFF,FF,F:FF,:FFFFFFFF:: NM:i:4 MD:Z:41G53T23T13T2 MC:Z:151M AS:i:118 XS:i:103 XA:Z:Test_seq,-213336,124M2I10M15S,7; A00126:224:H7HMWDSX3:3:1102:11306:1908 113 Test_seq 255993 42 125S26M = 185854 -70015 AGTAGTATTTAGCTATTCTTTCCCTCATATTTTCCAACAATGTCATTTTGCTCTAATTTTTCAAAAGTGTCTTCTGCTTTATTTTCCAGCTTATTGATTTTTTTATATTAGTAATAATTTTGATCGATCTAAATGCCCAATCAACCAACTT ,,FF,::F:F:,:FFF:,F:FF,FF,:F,FFFFF,F,F,FFFFFFFF:F,FF:F:F,FFFFFF::FFF,F,FFFFFFFFFFFFFF,,FFF:FFFFF,FFFFF:FFFFFFFFFFFF,FFFFFFFFF:FFFFF,F,F:FFFFFFFFFFFFFFF NM:i:0 MD:Z:26 MC:Z:151M AS:i:26 XS:i:0 SA:Z:Test_seq,185684,+,26S125M,60,3; A00126:224:H7HMWDSX3:3:1102:11306:1908 2145 Test_seq 185684 60 26H125M = 185854 321 GATCAAAATTATTACTAATATAAAAAAATCAATAAGCTGGAAAATAAAGCAGAAGACACTTTTGAAAAATTAGAGCAAAATGACATTGTTGGAAAATATGAGGGAAAGAATAGCTAAATACTACT FFFFFFFFF,FFFFFFFFFFFF:FFFFF,FFFFF:FFF,,FFFFFFFFFFFFFF,F,FFF::FFFFFF,F:F:FF,F:FFFFFFFF,F,F,FFFFF,F:,FF,FF:F,:FFF:,:F:F::,FF,, NM:i:3 MD:Z:68T17G1G36 MC:Z:151M AS:i:110 XS:i:0 SA:Z:Test_seq,255993,-,125S26M,42,0; A00126:224:H7HMWDSX3:3:1102:11306:1908 177 Test_seq 185854 60 151M = 255993 70015 ATGTGCCAGGTGCTGTTTTATGCTTTCATCTTCACTACTTCAGTCAGTCCTCACTGCAAACCAGTGAGGCAGATAGTATTATTCCCACTTTACAGTTGAAGAGACTGAGGCTCTATGAAATTAAGAAAATGTCCCCAGCCACAGGGCTAGT FFFFF:::F,F:FF:F:FFFF::FFF,FF,F,F:FFF:F:,F:FFFFFF:FFFFFF:FFF,:F::FFFF:FFFF:FFFFFFFFFFFFFFFFF:F:FF,FFFFFFF:FFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:31G119 MC:Z:125S26M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1102:7491:2785 97 Test_seq 168238 43 24S127M = 168516 429 CACTTGATGACCCAAAATCAGATTAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:2 MD:Z:33T74G18 MC:Z:151M AS:i:117 XS:i:97 XA:Z:Test_seq,-212674,127M24S,6; A00126:224:H7HMWDSX3:3:1102:7491:2785 145 Test_seq 168516 60 151M = 168238 -429 CAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAGTTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATT FFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:79A71 MC:Z:24S127M AS:i:146 XS:i:82 A00126:224:H7HMWDSX3:3:1102:22987:3724 97 Test_seq 288164 60 151M = 272431 -15711 TAAGGCATGGTAGAACCAGAGATGTCCCACTACACTGCTTTCCACCTGGGGACACTGGGTACGGGAAAGGTTCCCTTAGAGTTCTAGCTTTTCTTTTTTTGAAGAATTTATACAACAGGGAATTTTGAGAAGCCAGCTAAAATGCCCACGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:127S24M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1102:22987:3724 145 Test_seq 272431 30 127S24M = 288164 15711 CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTTAAAATGTACAGCCATCATGAAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:24 MC:Z:151M AS:i:24 XS:i:0 SA:Z:Test_seq,288412,-,128M23S,60,0; A00126:224:H7HMWDSX3:3:1102:22987:3724 2193 Test_seq 288412 60 128M23H = 288164 -376 CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:128 MC:Z:151M AS:i:128 XS:i:0 SA:Z:Test_seq,272431,-,127S24M,30,0; A00126:224:H7HMWDSX3:3:1102:11822:4335 113 Test_seq 166159 26 51M = 214826 48668 CAGGATAATGTAGGTCTCTGGTTCATGTCGCCCTTTCTGTCTCTTGGGTTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:28G22 MC:Z:51M AS:i:46 XS:i:38 XA:Z:Test_seq,+214826,51M,3; A00126:224:H7HMWDSX3:3:1102:11822:4335 177 Test_seq 214826 28 51M = 166159 -48668 GAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTG FF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFF NM:i:0 MD:Z:51 MC:Z:51M AS:i:51 XS:i:43 XA:Z:Test_seq,+166159,51M,2; A00126:224:H7HMWDSX3:3:1102:31313:5556 97 Test_seq 151016 60 151M = 222235 71249 TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCT FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFF NM:i:0 MD:Z:151 MC:Z:121S30M AS:i:151 XS:i:22 A00126:224:H7HMWDSX3:3:1102:31313:5556 145 Test_seq 222235 60 121S30M = 151016 -71249 CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGC FFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:30 MC:Z:151M AS:i:30 XS:i:0 SA:Z:Test_seq,151300,-,121M30S,47,0; A00126:224:H7HMWDSX3:3:1102:31313:5556 2193 Test_seq 151300 47 121M30H = 151016 -405 CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATC FFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:0 MD:Z:121 MC:Z:151M AS:i:121 XS:i:101 SA:Z:Test_seq,222235,-,121S30M,60,0; XA:Z:Test_seq,-48995,15M2I104M30S,4; A00126:224:H7HMWDSX3:3:1102:31394:5541 97 Test_seq 151016 60 151M = 222235 71249 TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFF:F:FFF NM:i:0 MD:Z:151 MC:Z:121S30M AS:i:151 XS:i:22 A00126:224:H7HMWDSX3:3:1102:31394:5541 145 Test_seq 222235 60 121S30M = 151016 -71249 CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGC FFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFF,,FFFFFFFF NM:i:0 MD:Z:30 MC:Z:151M AS:i:30 XS:i:0 SA:Z:Test_seq,151300,-,121M30S,47,0; A00126:224:H7HMWDSX3:3:1102:31394:5541 2193 Test_seq 151300 47 121M30H = 151016 -405 CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATC FFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFF NM:i:0 MD:Z:121 MC:Z:151M AS:i:121 XS:i:101 SA:Z:Test_seq,222235,-,121S30M,60,0; XA:Z:Test_seq,-48995,15M2I104M30S,4; A00126:224:H7HMWDSX3:3:1102:4218:5822 81 Test_seq 170436 60 151M = 170181 -406 TAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGT FFFFFFFFFFFFF::F:FFFF,FFFFFFFFF:FFFFF:FFFFFF,FFFFFFFF:FF:FFFF,FFF:FFFFF:FFFFFFFFFF:FFFFF,F,FFFF,F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FF,FFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:25 A00126:224:H7HMWDSX3:3:1102:4218:5822 161 Test_seq 170181 60 151M = 170436 406 TTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATGTTTTTTTACTTCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTC FFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFF:FF:F:FFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFF,F,:FFFFFFFF::FFFFFFF,:FFFF:FF,FFF,FFFFFFF::,F::F:F:FFF:FFFFFFFFF,F:,FFFFF:FF:, NM:i:4 MD:Z:15A78A7C1A46 MC:Z:151M AS:i:131 XS:i:55 A00126:224:H7HMWDSX3:3:1102:18602:6652 81 Test_seq 171528 60 151M = 171219 -460 TGTTTCCCTGGTCCTCCAGAAGTCCCAGCCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGTGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCCTGCAACTTGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:2A25T26T16C6T59T8A2 MC:Z:151M AS:i:120 XS:i:53 A00126:224:H7HMWDSX3:3:1102:18602:6652 161 Test_seq 171219 60 151M = 171528 460 AAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF: NM:i:1 MD:Z:141G9 MC:Z:151M AS:i:146 XS:i:79 A00126:224:H7HMWDSX3:3:1102:15619:9721 81 Test_seq 170227 60 151M = 170074 -304 ATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCG FF:FFFF::FF:FF,FFFF:FFF:F,F:FFFFF,FFF,FFF:F:FFFFFFFF:F,,FFF:FFF:FFFF:F:::FF,FFF::F,FFFFFF::F,FFFFFFFFFFFFFFF:F:FFFFFFFFFF:FFFFFFFFFFFF,FFFF:,:FFF:F,FFF NM:i:1 MD:Z:35A115 MC:Z:151M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1102:15619:9721 161 Test_seq 170074 25 151M = 170227 304 AAAATTTGTTTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAG ,,:F::FFFFFFFFFF:FFFFFF,FFFFFFFF::FFFFFF::::::FFF:FF:F:FFFFFFFFFFFF:FFF:FF::FFFFFFFF:FFF:FFF,,FFFFFFF:FFF:FFFFFFFFF:FF:FFFFFFFFF:FFFF:FFFFFFFF:F,FFFFFF NM:i:7 MD:Z:8A24C7G20A32A25C0A28 MC:Z:151M AS:i:116 XS:i:102 XA:Z:Test_seq,-38508,147M4S,9; A00126:224:H7HMWDSX3:3:1102:20645:10473 97 Test_seq 170889 60 151M = 171166 401 CGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTC FFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFF:FFFFFFFF,FF,FF:FFFFFFF::FF,FFF::FF,,FFFFFFFFFFFFFFFFFFFFF,F::FF,F,F:FF NM:i:1 MD:Z:70A80 MC:Z:124M27S AS:i:146 XS:i:23 A00126:224:H7HMWDSX3:3:1102:20645:10473 145 Test_seq 171166 60 124M27S = 170889 -401 TCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCACTCAAGAAGTACATTTTTCAATGATAA FFFF:FFFFF,FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFF:FF:FFFFFFFFFFFF,F:F:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:124 MC:Z:151M AS:i:124 XS:i:52 A00126:224:H7HMWDSX3:3:1102:15085:10864 81 Test_seq 366034 60 151M = 365724 -461 TTAACAAATTATGGATAACATTGGGAAGAATGGGAATTCCAGAACAATTAATTGTGCTCATAAGAACCTGGACATAGACCACGAGGCAGTCATTCGAACATGACAAGGGTATACTTTGTGGTTTAAAATCAGGAAAGGGGTTCCTCCAAGT FFFFFF,FF:FFFFF::F:F,FFFFFFFFFFFFFFFFFFFFF:FFFFF:FF:FFFFFFF,FF:F:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:F:FF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1102:15085:10864 161 Test_seq 365724 60 151M = 366034 461 AAATGAAATTCCACTTGTATTTTACTGCACACTCATAAAACAAGAAAAACACATGAGTTGTTTTTAAAGCCAATGAAATGCAGAGATTATCAAACAATATCGTTAATATGACACGCAAGTAAAATTATACTGAAGATCACTCAAAAACGGT FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,F:FFFFFFF:FFFFFFF:F:FFFFFF:FFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFF,FFFFFFFFFFFF:FFFF,FFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1102:7925:11616 81 Test_seq 168554 60 122M29S = 168515 -161 TTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTGTTGATAAGATCTGAGTTTTATTTTACTAAACATTATCCAC :FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:110A11 MC:Z:151M AS:i:117 XS:i:71 A00126:224:H7HMWDSX3:3:1102:7925:11616 161 Test_seq 168515 60 151M = 168554 161 TCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:149A1 MC:Z:122M29S AS:i:149 XS:i:74 A00126:224:H7HMWDSX3:3:1102:31575:12649 81 Test_seq 213195 10 63M = 213195 -63 CCTGATTTCAGAACCTATTATACAGCCACAGTAGTCAAAACAGCCTGGTACTGGTACAACAAC FFFFFFFFFFFFFFFFFFF::,FFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:26T36 MC:Z:63M AS:i:58 XS:i:53 XA:Z:Test_seq,+167781,63M,2;Test_seq,+152050,63M,3; A00126:224:H7HMWDSX3:3:1102:31575:12649 161 Test_seq 213195 12 63M = 213195 63 CCTGATCTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFF:F NM:i:1 MD:Z:6T56 MC:Z:63M AS:i:58 XS:i:53 XA:Z:Test_seq,-167781,63M,2; A00126:224:H7HMWDSX3:3:1102:20039:13150 65 Test_seq 213570 0 54M = 167411 -46160 CCAAAAAAGACGAAGAGAAACCAGATAACTGGGAGCTCCTAAAAAACAAACACC ,:FFF::,FF,F:FF:F:F,FFFFFFF,F,F,,F,,:,FF,FF,F,:FF,FF:F NM:i:3 MD:Z:7C2T34T8 MC:Z:54M AS:i:39 XS:i:39 XA:Z:Test_seq,-167411,54M,3; A00126:224:H7HMWDSX3:3:1102:20039:13150 129 Test_seq 167411 13 54M = 213570 46160 GGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCGTCATTTTTGG FFF,FFFFFF:FFFFFFFFF::FFF,FFFFFFFF,F::FF:,,FFF:F,FFFF: NM:i:1 MD:Z:43A10 MC:Z:54M AS:i:49 XS:i:44 XA:Z:Test_seq,-213570,54M,2; A00126:224:H7HMWDSX3:3:1102:18783:13855 81 Test_seq 189069 60 151M = 188880 -340 CTTAACTTGAGAACCTCTTTGATAAATTTTTACAAAAGATCCATGGAGAAACAAAGACGTTTGTTTTAGTGATTGTGAAAGATGATGTAGGTATCTATAAAAAATGTAATTTAACTAGTGTCCACTCTTTGCCCTAAATTATATTCCTAAC ,FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1102:18783:13855 161 Test_seq 188880 60 151M = 189069 340 AATTCTGTTAGGACCTAAGTTTTAGATTACCTGTGAGCCTTTTTTCACTCAGAATAAAAAACTCATTCTGAGGATAAGTTCAAAGAGACTGAATGTTCAAATGTAATTCACCACCAACCATGTAAGGTGGCTTATTTCATGAATTTTTGAA FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,F NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1102:27326:13839 81 Test_seq 99773 60 151M = 99580 -344 AGTGCGGCCCCTCCTCATCACTATGCCCATGCTTATGGCCGAGCTGGGTGTCATGGGTCAGGAAGATGCTGGAGAGGGAAGTGACCCAGCGATGGCCTTCACAGGAAGCCAGAGTCCAGCAGCCAAGATCAAGAGTCCAGTTGGCCAGTGC :FFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFF:FF:FFFFF,FFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:15S136M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1102:27326:13839 161 Test_seq 99580 60 15S136M = 99773 344 ACCCAAGTTATGATCGATCTATTCCTGTGAAGACCACAGTCTAGAAAACCCTGTGGGGCAGTTCTGCTCTGTCACATGGGTTCACTGAGCAGGAATCACCTTGAGGATACCTAACAGAGCAAAAACAAGGAGTTACTGGTAAAAACCTGCA FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:136 MC:Z:151M AS:i:136 XS:i:28 A00126:224:H7HMWDSX3:3:1102:13756:14732 97 Test_seq 169110 35 151M = 169312 353 ATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATTCTTTGAATTTCATTTGGTTCTGTTGTG FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFF:FFFFFF:FFFFFFFF,FFF NM:i:8 MD:Z:51G1A37C11A1G2G19T12C9 MC:Z:151M AS:i:111 XS:i:91 XA:Z:Test_seq,+153408,136M15S,9; A00126:224:H7HMWDSX3:3:1102:13756:14732 145 Test_seq 169312 60 151M = 169110 -353 TCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTGATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTG FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:38A112 MC:Z:151M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1102:28845:17159 97 Test_seq 168418 60 151M = 168688 393 AGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCT FFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFF, NM:i:1 MD:Z:77T73 MC:Z:123M28S AS:i:146 XS:i:74 A00126:224:H7HMWDSX3:3:1102:28845:17159 145 Test_seq 168688 60 123M28S = 168418 -393 TCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCACACCTGGTATAAATCCCACTGGATAAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCCTGGTGGCATAATAGTTAAGCACT FFFFFFFFFFFF:F:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF NM:i:4 MD:Z:28T35T19T3C34 MC:Z:151M AS:i:103 XS:i:49 A00126:224:H7HMWDSX3:3:1102:10881:18176 81 Test_seq 169625 60 151M = 169339 -437 TCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCTCTAGTTTTATTGCCTTGTGATC FFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:9 MD:Z:3T11A22G70A12C4T5C2C11G2 MC:Z:151M AS:i:109 XS:i:0 A00126:224:H7HMWDSX3:3:1102:10881:18176 161 Test_seq 169339 60 151M = 169625 437 TCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTA FFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF,FFF:FFFFFFFFF,FFFFFFFFFFFFFFF,,FF:FFFFF,FFF,FFFFF::FFFFFFFFF NM:i:1 MD:Z:128G22 MC:Z:151M AS:i:146 XS:i:103 A00126:224:H7HMWDSX3:3:1102:21757:18286 81 Test_seq 168632 60 151M = 168369 -414 GTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTG F:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:84T66 MC:Z:151M AS:i:146 XS:i:66 A00126:224:H7HMWDSX3:3:1102:21757:18286 161 Test_seq 168369 60 151M = 168632 414 TAGCTTTACTTCCTCCTTACCAATCTGGATGATCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGTGGTGATAAAGGGCATCCTTGTCCGGTTCCCGTTCTCGAGGGAAGTGCTTTCAGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFF:FFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFF,FFFF:FFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:9 MD:Z:18G11A0C0C63C22T6T6A6A10 MC:Z:151M AS:i:106 XS:i:49 A00126:224:H7HMWDSX3:3:1102:22073:20713 81 Test_seq 168175 7 151M = 167953 -373 GCTACTGTGAACGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:11T84T10T43 MC:Z:151M AS:i:136 XS:i:131 XA:Z:Test_seq,+212713,151M,4; A00126:224:H7HMWDSX3:3:1102:22073:20713 161 Test_seq 167953 60 151M = 168175 373 TATGACATTGGTATTTGGATCGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTT FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF: NM:i:1 MD:Z:20T130 MC:Z:151M AS:i:146 XS:i:77 A00126:224:H7HMWDSX3:3:1102:18159:21324 81 Test_seq 171327 60 129M22S = 171187 -269 TCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCTTTTCCCATCCAAGAACT FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:73G15T39 MC:Z:151M AS:i:119 XS:i:0 A00126:224:H7HMWDSX3:3:1102:18159:21324 161 Test_seq 171187 60 151M = 171327 269 GGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCT FFFFFFFFFFFF:::FFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,F:FFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFF:F:FFFFFF:FF NM:i:0 MD:Z:151 MC:Z:129M22S AS:i:151 XS:i:72 A00126:224:H7HMWDSX3:3:1102:32226:21762 97 Test_seq 170191 60 85M1D66M = 170502 462 GTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTCAGTCTAAGGTGTGTCTCTTGTAGGCA FFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFF::FFFFFFFFF,F,F,FFFF,FFF,FFFFFF,F::FFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFF,,F:FFFFFFF:FFFFFFFFF,FFFFF:FFFFFFF:F,F,FFFFFFF: NM:i:4 MD:Z:4C0A79^T39A26 MC:Z:151M AS:i:133 XS:i:73 A00126:224:H7HMWDSX3:3:1102:32226:21762 145 Test_seq 170502 60 151M = 170191 -462 CACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCATTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTA F:FFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFF:FFF::FF:FF:FFFFFF:F NM:i:1 MD:Z:86G64 MC:Z:85M1D66M AS:i:146 XS:i:28 A00126:224:H7HMWDSX3:3:1102:26648:21903 113 Test_seq 168457 60 151M = 212728 44270 AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGG FFFF:FFFFFFF:FFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFF,:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF NM:i:1 MD:Z:38T112 MC:Z:149M2S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1102:26648:21903 177 Test_seq 212728 0 149M2S = 168457 -44270 AACTACTGAAACTAATAGAAGAGTTTGTCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACCGAAGAGGAAATAATCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATACT F::FFFF,:FFF:F:F:,F:,F:FFFF,FF:FFFFFFF,FFFFFFFFFFF:FF:FFFFF:::FFFFFFFFF:FFFFFFF:FF,F:FF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFF:FFFFFF:F:FFFFFFF NM:i:5 MD:Z:27G18G49T1A12C37 MC:Z:151M AS:i:124 XS:i:124 XA:Z:Test_seq,+168162,2S149M,5; A00126:224:H7HMWDSX3:3:1102:9109:22592 97 Test_seq 168345 60 151M = 168557 363 GATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGACATCCTTGTCTGGTTCCC FFFFFFF:FFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFF:F::FFFFFF:FFFFFFFFFFFFF,FFFFFFFFF:F:FFF:FFFFFFFF,:FF:F:FFFFFFF:FF:FF,FF,F:FFFFFF:F:FFFF::FFFF,F,,F:FFF NM:i:5 MD:Z:1G18A28T82G17T0 MC:Z:151M AS:i:133 XS:i:76 A00126:224:H7HMWDSX3:3:1102:9109:22592 145 Test_seq 168557 60 151M = 168345 -363 CATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTG FFFFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:106T44 MC:Z:151M AS:i:146 XS:i:87 A00126:224:H7HMWDSX3:3:1102:26431:27759 81 Test_seq 129640 60 151M = 129405 -386 CATCTTGACTCCTGCAGGTCACAAGAATAGTACAGGGATAGAGCATGCATTCCTAAGCCTGCCCTTCAACTGAACAGGAAATGGCAACTCTGGTCAAGAGTTTGTCCCCAAATGCCACTACAGTGTGCAGCAATGTTTCCTCTGGGCTGTG FFFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1102:26431:27759 161 Test_seq 129405 60 151M = 129640 386 GTACTTGAATCTGCTCAGCTCTTAATGGTTACAACTGTTAACCCACAATGTCAGAATGATTTTCACACTTGTAAATCCCTAGAACTTTAATCTGGCTGCAAAAGAAGTCCTAGGCTCTTTCATTTAGTGGTAACGATGGTGGCAGGCACCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1102:27534:28792 81 Test_seq 168494 60 151M = 168155 -490 CCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTC FFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:1T149 MC:Z:151M AS:i:149 XS:i:96 A00126:224:H7HMWDSX3:3:1102:27534:28792 161 Test_seq 168155 18 151M = 168494 490 AGTATTTTATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATATCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTATATGTTTATTTTATATCCTGAGACACTTCCAAACTCTTCTATTAGTTTCAG FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFF:FFFFFF:F::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F NM:i:7 MD:Z:14C34T11G37G15A0T7T26 MC:Z:151M AS:i:116 XS:i:106 XA:Z:Test_seq,-212733,151M,9; A00126:224:H7HMWDSX3:3:1102:24433:32565 97 Test_seq 169299 60 151M = 169564 416 CCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTT FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:1 MD:Z:10T140 MC:Z:151M AS:i:146 XS:i:106 A00126:224:H7HMWDSX3:3:1102:24433:32565 145 Test_seq 169564 60 151M = 169299 -416 AATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTGTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAG FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:5G58T6T4A22G51 MC:Z:151M AS:i:126 XS:i:66 A00126:224:H7HMWDSX3:3:1102:24343:32910 97 Test_seq 169299 60 151M = 169564 416 CCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTT FFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF NM:i:1 MD:Z:10T140 MC:Z:151M AS:i:146 XS:i:106 A00126:224:H7HMWDSX3:3:1102:24343:32910 145 Test_seq 169564 60 151M = 169299 -416 AATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTGTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAG FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:5G58T6T4A22G51 MC:Z:151M AS:i:126 XS:i:66 A00126:224:H7HMWDSX3:3:1102:24379:34413 97 Test_seq 169290 60 151M = 169489 350 TATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATT FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:102 A00126:224:H7HMWDSX3:3:1102:24379:34413 145 Test_seq 169489 60 151M = 169290 -350 ATTTGTTCAAGTTGTCAGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATAAAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:30C0C41T65T11 MC:Z:151M AS:i:131 XS:i:70 A00126:224:H7HMWDSX3:3:1102:2031:34757 113 Test_seq 167554 31 151M = 213592 46015 ATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCTCCAATTGACTGACACTGGTCCTT FFF:FFFFFF,:FF:FF:,FFFF:FFFFFF,:,FFF:FFFFFFFFF::F:,FFFFFFFF:F,F:F,FFFF,:FFF:F:FFF:FFFFF:FFF:FF,,FF:FF:,FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFF,FFF,FFFFF,FF NM:i:1 MD:Z:127C23 MC:Z:127M24S AS:i:146 XS:i:131 XA:Z:Test_seq,+213334,126M2I23M,4; A00126:224:H7HMWDSX3:3:1102:2031:34757 177 Test_seq 213592 7 127M24S = 167554 -46015 CGATAACTGGTATCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGTGAAAGAATTTTCAGCTATGACATCTCAGACCAGCGCCTGATCGATCTCAAACTTTTGCCTAGTAGA F,:FFF,F,,,F,F::FFFFFFF,FFFFFF,F,FF:,F:F,F,F:F,FFF:FFFFFFFF:FF:FFFFF::,:F::FF:F,F,F,,:FFF:FF:FF:,F,F,:F,FFFF,FFF,FFFFFFFF,FFFF,FFF,F,FFF,FFFF,F:FF::F:F NM:i:6 MD:Z:0A9G1G64T6G26C15 MC:Z:151M AS:i:101 XS:i:96 XA:Z:Test_seq,+167316,24S127M,7;Test_seq,+400737,24S114M13S,5; A00126:224:H7HMWDSX3:3:1102:12563:35650 81 Test_seq 347119 60 151M = 346775 -495 ATATAAACCAAAAAATAGATAAGATAGAAAGTATTAGGCTAAGTTATGGAGAGATTTTTGAGGAGTTTTTTTTTTTTAATTTTTATTGTGCTTGAAGTGAAAGTTTACAAATCAAGTCAGTCTCTCATAGAAAAATTTATATACACCTTGC FFF:FFFFF:FFFF:F::FF::FFFF,FF::FF:FF,,FF:F:FFFF:FF:FFFFFFFFFF:FF::FFFFFF::FFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:59 A00126:224:H7HMWDSX3:3:1102:12563:35650 161 Test_seq 346775 60 151M = 347119 495 GTTTCTTAGACATGGAAACCCTGGTGGTGTAGTGGTTAAGTGCTACGGGGCTGCTAACCAAGAGGTCGGCATTTCGAATCCACCAGGCACTCCTTGGAAACTCTATGGGGCAGTTCTACTCTGTCCTATAGGGTCGCTATGAGTCGGAATT FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFF:FFFF:F:FFFFFFFFF,FF:F,FF,FFF,F NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:77 A00126:224:H7HMWDSX3:3:1103:19000:1892 113 Test_seq 169281 60 151M = 400060 230658 TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:28T122 MC:Z:122S29M AS:i:146 XS:i:103 A00126:224:H7HMWDSX3:3:1103:19000:1892 177 Test_seq 400060 0 122S29M = 169281 -230658 CAACTAAATAGACCCATAAAAAAAAACGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAGATCTTATAATAGTGGTCTTATACAGTA FFFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFF:FF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:26A2 MC:Z:151M AS:i:26 XS:i:26 SA:Z:Test_seq,168996,+,28S123M,60,2; XA:Z:Test_seq,-166639,122S29M,1;Test_seq,+214367,18M1I10M122S,1; A00126:224:H7HMWDSX3:3:1103:19000:1892 2209 Test_seq 168996 60 28H123M = 169281 436 TAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCGTTTTTTTTTATGGGTCTATTTAGTTG FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFF:FF:FFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFFF NM:i:2 MD:Z:96T6G19 MC:Z:151M AS:i:113 XS:i:36 SA:Z:Test_seq,400060,-,122S29M,0,1; A00126:224:H7HMWDSX3:3:1103:30544:2378 81 Test_seq 168462 60 151M = 168278 -335 GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGA FFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:1 MD:Z:33T117 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1103:30544:2378 161 Test_seq 168278 0 151M = 168462 335 CTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGTTAGCTTTACTTCCTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCGTAATTGT FFFF:,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,:FFF:,F NM:i:12 MD:Z:4T53C0A6C0G0G18A2A25T7C18C6C0 MC:Z:151M AS:i:95 XS:i:95 XA:Z:Test_seq,-212610,151M,12; A00126:224:H7HMWDSX3:3:1103:25880:3098 81 Test_seq 183897 60 151M = 183557 -491 TCAATTCTCCGGTCTTTCTTAGTCATTGTTCAGCTTTCAAATGCATATTATGCGATTGAAAACACCATGGTTTGGGTCAGGTGCCCCTTAGTCCTCAAAGTGACATCTTTGCTTTTTAACACTTTAAAGAGGTCTTTTGCAGCAGATTTAC FFFF:FFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:64 A00126:224:H7HMWDSX3:3:1103:25880:3098 161 Test_seq 183557 60 151M = 183897 491 ATCCAGTTGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCAC FFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:30 A00126:224:H7HMWDSX3:3:1103:26639:3818 97 Test_seq 41455 6 151M = 214062 172758 TGGGCAAAGGATATGAACAGGCACTTCACTAAAGAAGACATTCAGGTAGCTAACAGATACATGAGGAAATGCTCATGATCATTAGCCATCAGAGAAATGCAAATCAAAACTATGACGAGATACCATTTCACCCCAACAAGGCTGGCATTAA :FFF:F:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F:FFFFFFFFFFFFFFFF:FF:FFFF:FFFFFF::FFFFFF:FFFFFFF,F:F NM:i:13 MD:Z:20A25C0G26T0C13T22C0A1T5T4C4T15C3 MC:Z:151M AS:i:87 XS:i:81 XA:Z:Test_seq,-49064,151M,14;Test_seq,-151371,151M,15;Test_seq,+258709,151M,16; A00126:224:H7HMWDSX3:3:1103:26639:3818 145 Test_seq 214062 60 151M = 41455 -172758 AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCGTC F,FFFFFF:FFF,FFFFFFFFFF:FF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFF,FFFFF:FFFFF,FFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:148A2 MC:Z:151M AS:i:148 XS:i:116 A00126:224:H7HMWDSX3:3:1103:11460:5400 97 Test_seq 168738 60 151M = 169083 496 AAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTATAATTTTCTTTTTTTGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FF::FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFFFF NM:i:1 MD:Z:133A17 MC:Z:151M AS:i:146 XS:i:87 A00126:224:H7HMWDSX3:3:1103:11460:5400 145 Test_seq 169083 60 151M = 168738 -496 TTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAAGTAGTGTTTTTCCAAGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCGTAGTAATCTGAAATGATT FFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFF,FFF,FFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:65G12G39C11A20 MC:Z:151M AS:i:131 XS:i:76 A00126:224:H7HMWDSX3:3:1103:4598:6042 81 Test_seq 394259 60 151M = 394049 -361 CTGTGGCTGTATCAGTACCAGGCTGTTCTGAGTACCATGGCTGTATAGTAGGTTCTAAGATCAGGTAGTATGAGGCCTCCAACTTTGTTCTTCTTCTGTAATGGCTTACTTTCCATAGGAGTTCAATGGCTGACTTTTTGGAAGGAGATTG FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:18S133M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1103:4598:6042 161 Test_seq 394049 60 18S133M = 394259 361 CTGAATTAGTCCAACTCAATCCATTTTGAGTTAGTTTTTGTGTATGGCGTGAGATATGGACCCTGTTTCATTTTTTTACAAATAGACATCCAGTTGTGCCAGCACCGTTTATTAAAGAGACTGTCTTTTCCCCATTTAATGGACTTCAGCC FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:F,FFFFFFFFF:FFFFFFFFFFF,FF:FFFFFFFF NM:i:0 MD:Z:133 MC:Z:151M AS:i:133 XS:i:0 A00126:224:H7HMWDSX3:3:1103:21197:7075 97 Test_seq 166043 60 151M = 166318 426 ATGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACAGCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTT FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF,F,FFFFFFF:F, NM:i:1 MD:Z:79T71 MC:Z:151M AS:i:146 XS:i:36 A00126:224:H7HMWDSX3:3:1103:21197:7075 145 Test_seq 166318 60 151M = 166043 -426 CCACAATTCAAAGTGGGATGCAGAGTGTTTCCATAATAGAATTATTTTGCCCATTGACTTAGAAATCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTT FFFFFFFFFFF:FFFF,FF:FFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFF:FFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:F:FFFFFFF::FFFFFFFF NM:i:6 MD:Z:24A5T21G11G2T10T72 MC:Z:151M AS:i:121 XS:i:41 A00126:224:H7HMWDSX3:3:1103:11695:7529 81 Test_seq 167468 52 151M = 167155 -464 TGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:74G76 MC:Z:151M AS:i:146 XS:i:121 XA:Z:Test_seq,-400931,151M,6; A00126:224:H7HMWDSX3:3:1103:11695:7529 161 Test_seq 167155 45 151M = 167468 464 ATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFF:FFF:FF:FFFF:FFFFFFF:F:FFFF,FFF NM:i:2 MD:Z:23A64T62 MC:Z:151M AS:i:141 XS:i:119 XA:Z:Test_seq,-213729,151M,7; A00126:224:H7HMWDSX3:3:1103:6180:8249 65 Test_seq 212798 60 14S132M5S = 167960 -44839 AAGAAACCCTAAGAATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACGAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTTACCAAGGATGTAAAAGACCTATACAAAGAAAACTATA FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFF:FFFFFFFFFF:FFFFFF:FFFFFFFFFFFF::FFFFFFFFFFFFFFFF NM:i:3 MD:Z:28A13C62A26 MC:Z:151M AS:i:117 XS:i:85 A00126:224:H7HMWDSX3:3:1103:6180:8249 129 Test_seq 167960 60 151M = 212798 44839 TTGGTATTTGGATTGGAAGTGCGTTATATGTATAAATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTCTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTATAGTT F,:FFFFFFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFF:F NM:i:1 MD:Z:34G116 MC:Z:14S132M5S AS:i:146 XS:i:46 A00126:224:H7HMWDSX3:3:1103:18521:9799 81 Test_seq 168163 0 151M = 167943 -371 ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATATCCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTC F:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:6C46G53A0T10T31 MC:Z:151M AS:i:126 XS:i:126 XA:Z:Test_seq,+212725,151M,5; A00126:224:H7HMWDSX3:3:1103:18521:9799 161 Test_seq 167943 60 151M = 168163 371 CCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGT FFF::F:FF:FFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF:FFFFFFFF: NM:i:1 MD:Z:117C33 MC:Z:151M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1103:18638:10066 81 Test_seq 168163 0 151M = 167943 -371 ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTATTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATATCCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:F NM:i:5 MD:Z:6C46G53A0T10T31 MC:Z:151M AS:i:126 XS:i:126 XA:Z:Test_seq,+212725,151M,5; A00126:224:H7HMWDSX3:3:1103:18638:10066 161 Test_seq 167943 60 151M = 168163 371 CCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGT FFFFFFFF:,FFFF:,:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF NM:i:1 MD:Z:117C33 MC:Z:151M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1103:30282:10160 81 Test_seq 351312 60 151M = 341142 -10321 TCTCTATCCTTCTTGTTTCTTTTTCCTTACCATTTGATGGTTGAAGAAACTGATCATTTTTTCTGTAGAGCTTCCCATATTCTAGGTTTAGGCGTCTCAGTGGTGTTGTGTAACACATTCTTCTGTCCCTTTATTTTCTGTAATTGGCTAC FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:30M121S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1103:30282:10160 161 Test_seq 341142 60 30M121S = 351312 10321 GCGGGGGCCTGAACCCTGCTCTGCACGATTGATCATACCTAAAATATTGGCAGTAATTACACTATCATCTATTTACAAATCATTGTTCATAGTTTCCAGTTGTCTCATAATTTTTTTTTTAATAGTTGAGTTGCTCAAAACAAGACCTAAA FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF,FF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:0 MD:Z:30 MC:Z:151M AS:i:30 XS:i:0 SA:Z:Test_seq,351127,+,29S122M,60,0; A00126:224:H7HMWDSX3:3:1103:30282:10160 2209 Test_seq 351127 60 29H122M = 351312 336 TGATCATACCTAAAATATTGGCAGTAATTACACTATCATCTATTTACAAATCATTGTTCATAGTTTCCAGTTGTCTCATAATTTTTTTTTTAATAGTTGAGTTGCTCAAAACAAGACCTAAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF,FF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:0 MD:Z:122 MC:Z:151M AS:i:122 XS:i:0 SA:Z:Test_seq,341142,+,30M121S,60,0; A00126:224:H7HMWDSX3:3:1103:28402:11537 81 Test_seq 168626 60 151M = 168347 -430 ATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATC FFFFFFFFF:FFFFFFFF:FFFFF,F:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:90T60 MC:Z:18S133M AS:i:146 XS:i:72 A00126:224:H7HMWDSX3:3:1103:28402:11537 161 Test_seq 168347 60 18S133M = 168626 430 ATTTGGATATTAATTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCACGATGTTGAATAAGAGCGGTGATAAAGGGCA F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF,,FFFFF:FFFFF:F,F,FFFFFFFFFFFFFFF,:FF,FFFF:FFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFF NM:i:3 MD:Z:18A21G59T32 MC:Z:151M AS:i:118 XS:i:71 A00126:224:H7HMWDSX3:3:1103:10149:12461 97 Test_seq 167571 27 151M = 167876 456 CCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCACTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:1 MD:Z:106T44 MC:Z:151M AS:i:146 XS:i:133 XA:Z:Test_seq,-213317,143M2I6M,4; A00126:224:H7HMWDSX3:3:1103:10149:12461 145 Test_seq 167876 60 151M = 167571 -456 TCAGTAATGCTTTGCTTATCCGGAGGTTCTTTCCCTTCCATATGAAATTAGTGATTTATTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTAT :FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFF:FFFFFFF:FFFFFFFF,F,F:FF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:23G33G93 MC:Z:151M AS:i:141 XS:i:46 A00126:224:H7HMWDSX3:3:1103:29125:12633 81 Test_seq 168432 60 151M = 168241 -342 GGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGG FFF:F:F,,FFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFF:F:FFFFF:FFFFFFFFFFFFFF:FF,FFFFF,FF:FFFFFFFFFFF:F:FFF,FFFF:FF:FFFFFFFFFF:FFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFFFFFF NM:i:1 MD:Z:63T87 MC:Z:103M4I42M2S AS:i:146 XS:i:80 A00126:224:H7HMWDSX3:3:1103:29125:12633 161 Test_seq 168241 36 103M4I42M2S = 168432 342 CCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTAA FFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFF:F:F:FFFFFFFFFFF,:F,FFFFFFFFFFFF,F:,F,FFFFFFFF,FF:,FFFFF:FFFFFFFFF:F:F,FFFFFFFFFF:FFFF,FFFFFFFF,,FFF:,F NM:i:8 MD:Z:30T10T63G18A20 MC:Z:151M AS:i:115 XS:i:95 XA:Z:Test_seq,-212653,2S37M4I108M,12; A00126:224:H7HMWDSX3:3:1103:17797:13119 97 Test_seq 261103 0 66M = 261103 66 CTTGTGTCCTTGGTGTTCTTCTTTCTCCTTTGGTCCAGGTGAGTTGAGACCAATTGATGCATCTTA FFF:FFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:4 MD:Z:21A10A8G11C12 MC:Z:66M AS:i:46 XS:i:46 XA:Z:Test_seq,+399637,66M,4;Test_seq,+150455,66M,5;Test_seq,-130422,57M9S,3;Test_seq,+48182,66M,5; A00126:224:H7HMWDSX3:3:1103:17797:13119 145 Test_seq 261103 31 66M = 261103 -66 CTTGTGTCCTTGGTGTTCTTCATTCTCCTTTGATCCAGGTGGGTTGAGACCAACTGATGCATCTTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:66 MC:Z:66M AS:i:66 XS:i:56 XA:Z:Test_seq,-399637,66M,2;Test_seq,-150455,66M,3; A00126:224:H7HMWDSX3:3:1103:8296:13730 81 Test_seq 151727 0 59M = 400921 249137 TTCTTTATAATGTTTTGTATACTGTTTATGCCATGTATTAGGGCTCCTAACATTGTCCC FFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:5A42C10 MC:Z:59M AS:i:49 XS:i:49 XA:Z:Test_seq,-49420,59M,2;Test_seq,-19079,59M,4;Test_seq,+213518,52M7S,2; A00126:224:H7HMWDSX3:3:1103:8296:13730 161 Test_seq 400921 31 59M = 151727 -249137 TTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAACGTTGTGCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:56C2 MC:Z:59M AS:i:56 XS:i:46 XA:Z:Test_seq,-213518,59M,3; A00126:224:H7HMWDSX3:3:1103:32651:15358 81 Test_seq 168695 60 151M = 168430 -416 TTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATC FFFFFFFFFF:F::FFFFFFFFFF:FFFFFFFFFFFFF:FF:FF,FFFF:FFFFFFF:FFFFFFF:FF::FFFFFF:FFFFFFFFFFFF::FFFFF:FFFF:FFFFFFFF:FFF,FF:FFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF NM:i:1 MD:Z:21T129 MC:Z:151M AS:i:146 XS:i:74 A00126:224:H7HMWDSX3:3:1103:32651:15358 161 Test_seq 168430 60 151M = 168695 416 CTGGCTAAGACTTCCAACACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATCTTGGCTGTTGGCTTTGCATAGATGCCCTGTAGTATGTTGA FFFFF,:::FFFFFFFFFFFF,,F,,F,F:FFFFFFFF,FFF:FFFFF:FFFF,,FFFFFFFFFFFF:FFFFFFFFFFF,FFFF,FFFFF,,F:FFFF:,,:,F:FFF,:,::FFF,F::FFF::F:FF,:FFFFFFF,,,:,,F,:F:FF NM:i:8 MD:Z:7G6A1G0T47T44A28T2T8 MC:Z:151M AS:i:113 XS:i:69 A00126:224:H7HMWDSX3:3:1103:13693:17065 81 Test_seq 170264 60 151M = 170079 -336 TTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATT FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:1 MD:Z:112C38 MC:Z:151M AS:i:146 XS:i:80 A00126:224:H7HMWDSX3:3:1103:13693:17065 161 Test_seq 170079 60 151M = 170264 336 TTGATTTATGTATCTTGCAGCCCTGTCATTGGGTGCGTAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAATCTATTTTGTCAGAAATT FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF,FFFF:F::FFFFFFFFFFFFFFF,FFF NM:i:5 MD:Z:28C28A58C0A14G18 MC:Z:151M AS:i:126 XS:i:87 A00126:224:H7HMWDSX3:3:1103:31503:17127 97 Test_seq 126887 60 151M = 127146 410 AGATATTTTTATATCACATTTCAGTTATCACACATATTTTGAAATATCCTTCGCACTTATCACCGAGTCAGAATTTTGGAGCTTAATAGGCCCCCAGACATCCCTGGGTGGTGTAGGCAGTTAAACACTCAGCTGCTAACTGAAAGGTTGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1103:31503:17127 145 Test_seq 127146 60 151M = 126887 -410 AGGGTCGCCATGAGTTGGGGCTGGTGCTGTGGCAGCTGTTGTTGTTTGTTTGTTTGTTTTTATTAGACCCACAATGAAATCCTCTTATTTAATATATTTATTATAAATTTGTAATTATTAATAACTATGTCAGTATCATTGGTTTCTTGTG :FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1103:23981:17065 81 Test_seq 432931 60 130M21S = 432837 -224 GGAGCTTGCTATTATTTTTTTAATCTTTAACCACAAACTAAGCTTCATTGAAATACCTTTAAAAACTCTTGAGCATATATACCTGTGTGAGTAAACACATCAGTTGTTATTTGTTTCAGATATTCAGATCGATCATCTGAGTGAATTTCCA ,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF NM:i:0 MD:Z:130 MC:Z:151M AS:i:130 XS:i:0 A00126:224:H7HMWDSX3:3:1103:23981:17065 161 Test_seq 432837 60 151M = 432931 224 TAACGAGATGTGGAGTAAGGAACTGAGTAGCCTAGAGTGTGAGTTGTTAACCATAGTCCCATTTTGACTAAAGATTTTTATAAGCTGAGAAAAAGGAGCTTGCTATTATTTTTTTAATCTTTAACCACAAACTAAGCTTCATTGAAATACC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:130M21S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1103:24686:17315 81 Test_seq 169350 60 151M = 169076 -425 AATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGT :FFFFFFFFFFFFFFFF:FFFFF,FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:117G33 MC:Z:2S149M AS:i:146 XS:i:101 A00126:224:H7HMWDSX3:3:1103:24686:17315 161 Test_seq 169076 48 2S149M = 169350 425 AGTTACCGTTTCAATCTCTTTTTTTGTTATGCGTCTATTTAGTTGTTCTACTTCTGATTGTGTTAGTTTAGGTAGGTCGTGTTCTTCCAGGACTTCATCCATGTCTTCTAGGTTTGCAAATTTCTTAGAGTACAATTTTTCGTAATAATCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFF:FF:F:,FFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFF,,FFFFF,FFFF,FFF:F,FF:FFFFFFFFFFFF,FFFFFFFFFFFFFFF NM:i:13 MD:Z:29G25A19A5T3G1A2A9T12T7G3C11A4G6 MC:Z:151M AS:i:84 XS:i:52 A00126:224:H7HMWDSX3:3:1103:12192:17691 81 Test_seq 86728 60 151M = 86584 -295 TTTGCCATCATATTTAAGCAAGAGTTTTGCAGGATATATTATACTTCTTTGGCAGTTTTTATCTTTCAAGGTTTTATGTATGTCATCCTATCGCCCTCTTGCCTACATGGTTTTTGCCAAGTAATCAGAGCTTAGTCTTATTGTTTCCCCT FFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1103:12192:17691 161 Test_seq 86584 60 151M = 86728 295 CTGGTATGGTCTTGCGTCCTACATTCAGGCTATCATCTGATGTTGTTTGTTCTCAAACTGAAGGACTTCCTTTAATAATTCTTGTATGTTTGTTTTGGTTTTTATGTATTCCCTTAATTTCTCTTTATCTGGAAATGTCCTAACTTTGCCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:25 A00126:224:H7HMWDSX3:3:1103:27145:18161 97 Test_seq 316265 9 70M = 316265 70 AAACATCCTTCTGGTATTCTCTGCTTTCAGCCAGGATCCATCTGACATCAGCAATGATATCCCTGGTTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:70 MC:Z:70M AS:i:70 XS:i:65 XA:Z:Test_seq,+15178,70M,1;Test_seq,+333542,70M,1;Test_seq,-365919,70M,1; A00126:224:H7HMWDSX3:3:1103:27145:18161 145 Test_seq 316265 7 70M = 316265 -70 AAACATCCTTCTGGTATTCTCTGCTTTCAACCATGATCCATCTGACATCAGCAATGATATCCCTGGTTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:29G3G36 MC:Z:70M AS:i:60 XS:i:55 XA:Z:Test_seq,-15178,70M,3;Test_seq,+365919,70M,3;Test_seq,-333542,70M,3; A00126:224:H7HMWDSX3:3:1103:4661:20400 81 Test_seq 170394 60 46M10I95M = 170269 -266 TATTGGTGCATTTTGTCCATTTACATTCAGTGTAATTATAGATAAATAATTTTTTTTAAGTTTTTAGTGCTGTCATTTTGATGCATTTTCTTGTGAGTTTTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTA FFFFFFFF:FFF:,FFF:FFFF,,FFFF,FFFFFFFFFFF:FF::FF,:FFF:FFFFFFFF:FFF:FF:,:FFFFFFFF:::F,,FF:F:,FFFF,FFF:FF,:::FFFF,FFFFFF::,:FF:,FFFFF:,FFFF,F:F,,FFFFF,FFF NM:i:17 MD:Z:13A16C43C5A4T3G6T44 MC:Z:151M AS:i:90 XS:i:25 A00126:224:H7HMWDSX3:3:1103:4661:20400 161 Test_seq 170269 60 151M = 170394 266 TGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTTCATTTAGTCCATTTACAT FF:F:FFFFFFFFFFFFFFF,::F,FFFFFFF:FFFF:FFFFFF:F:F:F:FF:FF,FF:F::FF,FF:FFFFFFFFFFFF:FF,FFF,,FFFFFFFFFFFF,F:F,,,FFFF:FFFFFFF,FFF:FFF:F:,FF:::FFFFFF:FFF,FF NM:i:1 MD:Z:132G18 MC:Z:46M10I95M AS:i:146 XS:i:75 A00126:224:H7HMWDSX3:3:1103:23619:20572 81 Test_seq 47367 60 151M = 47127 -391 CTTCCAAATTTTTTGGCATAGATCAGTGAGTGCTTCCAGCACTGCATCTGTTTGTGGAAACATCTCAACTGGTATTCTGTCAATTCCTGGAGCCTTGTTTTTCACCAATGCCTTTAGTGCACATTGGACCTCTTCCTTGAGTCCAAGTTCT F:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:42 A00126:224:H7HMWDSX3:3:1103:23619:20572 161 Test_seq 47127 60 151M = 47367 391 GATCTTTTTGACATCAGCAATGATATCTTTTGTTATACATCCTCTTCTGACTCTGCCTTGGATTTCTGGCAGTTCTCTGTCAATGTACTGCTGCAAACACTTTTAAATGATCTTCAGCAAAATTTTACTTGTGTGTGATATTAACGATATT F:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF NM:i:1 MD:Z:50A100 MC:Z:151M AS:i:146 XS:i:46 A00126:224:H7HMWDSX3:3:1103:18114:21433 81 Test_seq 167513 58 151M = 167400 -264 ATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTT FFFFFFFFFFFFFFFFFFFFF:F,FFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:124 XA:Z:Test_seq,+213375,85M2I23M2I37M2S,5; A00126:224:H7HMWDSX3:3:1103:18114:21433 161 Test_seq 167400 60 151M = 167513 264 AGATGAGCATAGGTGTTTGATTTCTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTATGTTTATGCCCTGTGTTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:3 MD:Z:23T55C14A56 MC:Z:151M AS:i:136 XS:i:106 A00126:224:H7HMWDSX3:3:1103:22417:22654 81 Test_seq 167403 0 134M17S = 167167 -370 TGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCGTTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTAAATGGTCTGAATTCAA F:FFFFFF:FFF,FFFFFFFFFFFFFFFFFF,:F,,:F::FFFFFFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:39T14A29G2C22A0T2T19 MC:Z:151M AS:i:99 XS:i:99 XA:Z:Test_seq,+213498,17S134M,7; A00126:224:H7HMWDSX3:3:1103:22417:22654 161 Test_seq 167167 52 151M = 167403 370 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA :FFFFFFFFFFFFFF,FFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:,F::,FFF:F,F,FF,FF,FFF,FFF,FF,F::FF:F::FFF,FFFFF:FF,:FF:FF:FFFFFFFFFFFFF,:FFFFFFFF:FFF NM:i:1 MD:Z:76T74 MC:Z:134M17S AS:i:146 XS:i:121 XA:Z:Test_seq,-213717,151M,6; A00126:224:H7HMWDSX3:3:1103:8368:23093 81 Test_seq 167414 60 151M = 167067 -498 GTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGCCTTTATGTTTAGGTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:135T15 MC:Z:151M AS:i:146 XS:i:106 A00126:224:H7HMWDSX3:3:1103:8368:23093 161 Test_seq 167067 20 151M = 167414 498 GCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGTCTAGTTGGTGTGAGATGGAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTAGCTGCTTGAATATCT FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F,FFFFFFFFF:FFFFFF:FFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFF:FFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFF,F NM:i:3 MD:Z:77C57G5C9 MC:Z:151M AS:i:136 XS:i:126 XA:Z:Test_seq,-213817,151M,5; A00126:224:H7HMWDSX3:3:1103:1615:23484 97 Test_seq 166033 60 151M = 166126 244 TGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGCCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGTTCTCTGGTTCA FFFFFFFFFFFFFF:FF:FFF:::FFFFFFF:,:FFFF,F,FF,FF,FFFF,FFFFFFFFFF:FF:F:FF:FFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFF,FF:FFFFFFF: NM:i:5 MD:Z:39T36G0A23G37G11 MC:Z:151M AS:i:126 XS:i:61 A00126:224:H7HMWDSX3:3:1103:1615:23484 145 Test_seq 166126 60 151M = 166033 -244 TTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATG FFFFFFFF:FFF,::FFFFFFF::FFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,FFF NM:i:1 MD:Z:8G142 MC:Z:151M AS:i:146 XS:i:82 A00126:224:H7HMWDSX3:3:1103:17915:23719 97 Test_seq 169352 60 151M = 169470 269 TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTCATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF,,F,,:F:FFFFFFFFFFFFFFFFFF:FF:F:FFFFFFF:FFF::F:FFFFF,FF:::FFFFFFFFFFFFFFF,FFFF, NM:i:1 MD:Z:79A71 MC:Z:151M AS:i:146 XS:i:96 A00126:224:H7HMWDSX3:3:1103:17915:23719 145 Test_seq 169470 60 151M = 169352 -269 CTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTCGGGACATTTCTCTGATTTTAGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATATAAATTGGCCTCTGAGCACCGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTT FFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:35A5G7C0C3G56T39 MC:Z:151M AS:i:121 XS:i:69 A00126:224:H7HMWDSX3:3:1103:25238:24126 97 Test_seq 168808 60 27S124M = 168992 335 TGTGCAAGGGCTCTGATGACTAAGACTATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGCAATGTCTTTACCTGGTTTTGGTATCAGGGAGATGGTAGCTTCAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF:FFFFFF:FFFFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFF,FFFFFF:FF NM:i:3 MD:Z:79T26A9G7 MC:Z:151M AS:i:109 XS:i:75 A00126:224:H7HMWDSX3:3:1103:25238:24126 145 Test_seq 168992 60 151M = 168808 -335 GTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCCGAATGTGTTAGTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:136T14 MC:Z:27S124M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1103:31069:25551 81 Test_seq 185755 60 151M = 185527 -379 AGAGCAAAATGACATGGGTGGAAAATATGAGGGAAAGAATAGCTTAATACTACTACTAATAAAAATCAGAACATCTAACATATTCTGCAGGCTCTTAGTATGTGCCAGGTGCTGTTTTATGCTTTCATCTGCACTACTTCAGTCAGTCCTC ,FF:F:F,::FF,,F:F:F::FFF,FFFFF,FF,,,,FF,,,:F,FF:F,FF:,FFF,FFF,,:FF:FFF::F,:F,F,,,F:F,FFF,,F,,FFF,FFFFF,::,FF:FF:F:FFFF:F:,FFFF:F,FF:FF::,:F,,:F:,FF,,F, NM:i:3 MD:Z:44A28G18A58 MC:Z:151M AS:i:136 XS:i:0 A00126:224:H7HMWDSX3:3:1103:31069:25551 161 Test_seq 185527 60 151M = 185755 379 GATCAGTCCAATGTGTGCCAAACCACCAATAATCATAACATAGCCAAGCTGACACAAAATATTAACATCACACACAGAGCAATTCAAGAACTACATCCATAAAACCAAGAAAACTGTATAAAAAAGAGCAACCTGTGAATAAAAAATTGAT ,:FFFF,FFFFFF,:,:FFFFFFFFFFFF,FF,F::FF:FFFFFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFFFFFFF,FFF,FFF:FFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFF:FFFFFF,:FF:FF,F,F,FFFFFFFF,:,: NM:i:1 MD:Z:149T1 MC:Z:151M AS:i:149 XS:i:0 A00126:224:H7HMWDSX3:3:1103:31901:25989 81 Test_seq 168665 60 114M37S = 168499 -280 TTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGTCTCAGATTATTCTTAAGGTTTCTTCTTTTTTTTGTG ,FFF:FFF,FFF,FFFFFFFF:FFFFFFFFFFFFFF:FFFFFF:FFF:FFFFFF,FFFFFFFF:FF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF NM:i:2 MD:Z:22A28T62 MC:Z:151M AS:i:104 XS:i:58 A00126:224:H7HMWDSX3:3:1103:31901:25989 161 Test_seq 168499 60 151M = 168665 280 CTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATG FFFFFFFFFFFFFFF,FF:FFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,F,,F:FFFFFFFF:FF:FFFFFFFFFFFFF,F,F:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:137A13 MC:Z:114M37S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1103:15112:26819 97 Test_seq 170019 29 151M = 170289 421 GTTGAAGTCTCCTACTATAATTGTGGAGGTGTCTATCTCACTTTTCAGTTCTGATAAAATTTGATTCATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATG FFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFF:F:F:F:FFFFFFFFFFFF:FFFFFF:FFFF,F,FFFFFFFF,:FFFFFF,F:FF NM:i:11 MD:Z:9C10A7A10G7A5T12T21C7G20A32A0 MC:Z:151M AS:i:100 XS:i:82 XA:Z:Test_seq,-38563,147M4S,13; A00126:224:H7HMWDSX3:3:1103:15112:26819 145 Test_seq 170289 60 151M = 170019 -421 TTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCTTAATTATAGATAAA FF:FFFFFFFFFF,F:F,FFFF:FFFFFFFFFFFFF:FF::FFFF,::,FF:FF:FFFF:FFFFFFF:F:F::FFFFF:FFFFFF:FFF:FFF:FF::FFFFFFF:F:::FFFFFFF,FF:FFFF,:FFFFFFF:F:FFFFFF:,FFFFFF NM:i:1 MD:Z:136G14 MC:Z:151M AS:i:146 XS:i:84 A00126:224:H7HMWDSX3:3:1103:6307:27007 81 Test_seq 167416 22 120M31S = 167150 -386 TTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCAGTCATACAGTAATTCTGTGTTTCACTTTTG FFFFFFF:F:FFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:97A0T21 MC:Z:151M AS:i:110 XS:i:100 XA:Z:Test_seq,+213499,31S120M,4; A00126:224:H7HMWDSX3:3:1103:6307:27007 161 Test_seq 167150 42 151M = 167416 386 TTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAT FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::,FFFFFFF,FFF::F:FFFFF:FFFFF,FFFFFFFF:F, NM:i:1 MD:Z:93T57 MC:Z:120M31S AS:i:146 XS:i:126 XA:Z:Test_seq,-213734,151M,5; A00126:224:H7HMWDSX3:3:1103:27597:28244 81 Test_seq 260971 7 74M = 260971 -74 CAGGGGTTCTCTTTTGTGTTCCCTGTCTGGGCAGTCATCTGTTGTAGCCGGGCACCATCTAGTTCTTCTGGTCT FFFFF,FFFFFF,FFFFFFFFFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:12G14A11G8T25 MC:Z:74M AS:i:54 XS:i:49 XA:Z:Test_seq,-48050,74M,5;Test_seq,-150323,74M,5; A00126:224:H7HMWDSX3:3:1103:27597:28244 161 Test_seq 260971 42 74M = 260971 74 CAGGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCGGTTGTAGCCGGGCACCATCTAGTTCTTCTGGTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:: NM:i:1 MD:Z:48T25 MC:Z:74M AS:i:69 XS:i:54 A00126:224:H7HMWDSX3:3:1103:4191:28541 65 Test_seq 40015 18 4S147M = 168498 128484 CATGAATAAAACAAAAGACAAGAATCACATGATTTTATCAATTGATGCAGAAAAGGCATTTGACAAAGTTCAACACTCATTCATGATAAAAACCCTCAGCAAAATAGGAATAGAAGGAAAATTCCTCAACATAATAAAGGGCACTTATACA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:25A23G22C16T10C38T7 MC:Z:151M AS:i:117 XS:i:107 XA:Z:Test_seq,-152855,151M,9; A00126:224:H7HMWDSX3:3:1103:4191:28541 129 Test_seq 168498 60 151M = 40015 -128484 TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFF NM:i:1 MD:Z:138A12 MC:Z:4S147M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1103:11668:28620 81 Test_seq 169311 60 151M = 169162 -300 TTCTTTAGTCAGTCTAGCTAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:18C132 MC:Z:26S125M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1103:11668:28620 161 Test_seq 169162 60 26S125M = 169311 300 TCGTCAAAAAGAACGTTTCAAGATCTAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACGTAGTAATCTGAAGTGATTCTTTTAATTTCATTTGGCTCTGTTGTGATGTGGTCCTTCTCATTTCTTATTCG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFFFFFFF::FFFFF:FF:F NM:i:5 MD:Z:7C31C26A39T6G11 MC:Z:151M AS:i:100 XS:i:61 A00126:224:H7HMWDSX3:3:1103:13485:30545 113 Test_seq 212649 27 151M = 168465 -44185 GGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGAT FF:FF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:7 MD:Z:8G8A5T20C0T17T61G25 MC:Z:151M AS:i:116 XS:i:101 XA:Z:Test_seq,+168239,151M,10; A00126:224:H7HMWDSX3:3:1103:13485:30545 177 Test_seq 168465 60 151M = 212649 44185 CGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTT FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:30T120 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1103:24207:31986 97 Test_seq 227720 60 151M = 227933 331 TGTTTCCTGTCTGTTGATCTCTGACAGGAGTTCAAGACGGCACATCAGTGCTGCATTAGCTGACGGGGGACCTCTGCTCATATCTTTCCTCACTCTCTGTTCTCAGTTTCTTATTCCTCAGTGCTTGATTGAATTCTTTATCTCTTCATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,F:FFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:118M33S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1103:24207:31986 145 Test_seq 227933 60 118M33S = 227720 -331 GTGGAGGTACAGTGTGGTGCTTCTGTTTATAGCACCATGTTGCCTCTGTCCCTGACTGTACATTTTTGTAATGTCCACTCAATTCATGTGCATGGAATTTGATTTATTCTGGAGGTTAGATCACCGACCCTGGAAGCAGCAGTCAAGGAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:118 MC:Z:151M AS:i:118 XS:i:0 A00126:224:H7HMWDSX3:3:1103:24966:32299 81 Test_seq 171229 60 151M = 170889 -491 TTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACT FFFFF:FFF:FFFFF:FFF,FFFFFFFFFFF:F,FFFF:FFF:FFFFFFF:F:FFFFFFFF:FFFFF:FFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,F:FFFFFFFFFFFFF,FFFFFFFFFFF:FFF NM:i:1 MD:Z:140G10 MC:Z:151M AS:i:146 XS:i:72 A00126:224:H7HMWDSX3:3:1103:24966:32299 161 Test_seq 170889 60 151M = 171229 491 CGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTGTTGGTCATCAGGCCGAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTC FF:FFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF,F:FF::FF,:FFFFFFFFFFFFFFFFFFFFF,:,:FFFFFFFFFFFFFFF:FFF:FFFF,F:, NM:i:3 MD:Z:42A14A12A80 MC:Z:151M AS:i:136 XS:i:23 A00126:224:H7HMWDSX3:3:1103:24894:32424 97 Test_seq 227720 60 151M = 227933 331 TGTTTCCTGTCTGTTGATCTCTGACAGGAGTTCAAGACGGCACATCAGTGCTGCATTAGCTGACGGGGGACCTCTGCTCATATCTTTCCTCACTCTCTGTTCTCAGTTTCTTATTCCTCAGTGCTTGATTGAATTCTTTATCTCTTCATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:118M33S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1103:24894:32424 145 Test_seq 227933 60 118M33S = 227720 -331 GTGGAGGTACAGTGTGGTGCTTCTGTTTATAGCACCATGTTGCCTCTGTCCCTGACTGTACATTTTTGTAATGTCCACTCAATTCATGTGCATGGAATTTGATTTATTCTGGAGGTTAGATCACCGACCCTGGAAGCAGCAGTCAAGGAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:118 MC:Z:151M AS:i:118 XS:i:0 A00126:224:H7HMWDSX3:3:1103:14299:32863 81 Test_seq 170346 60 151M = 170143 -354 TATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCAT FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:1 MD:Z:144T6 MC:Z:13S138M AS:i:146 XS:i:48 A00126:224:H7HMWDSX3:3:1103:14299:32863 161 Test_seq 170143 60 13S138M = 170346 354 GAACAGTGATGAATGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGGTACTCCTGCTCTTTTTTGCTTATTGTTTGCTTGATATATTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFF:FFFF NM:i:5 MD:Z:53A40C7C0T15A18 MC:Z:151M AS:i:113 XS:i:66 A00126:224:H7HMWDSX3:3:1103:8558:33066 97 Test_seq 167149 42 151M = 167404 388 ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAA FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFFF:FFFF:,F:FFFF:FFFFFFFFFFFF NM:i:1 MD:Z:94T56 MC:Z:133M18S AS:i:146 XS:i:126 XA:Z:Test_seq,-213735,151M,5; A00126:224:H7HMWDSX3:3:1103:8558:33066 145 Test_seq 167404 60 133M18S = 167149 -388 GAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTAAAGCAATCTTTTGCAGC FFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:38T44G2C22A0T2T19 MC:Z:151M AS:i:103 XS:i:71 A00126:224:H7HMWDSX3:3:1103:12463:35008 97 Test_seq 137147 60 151M = 137484 461 GCATATTTCAGATTGTTAATGAGCCCTCCTCCAGTCTTGATGCTGCATTCTTCATATTATCCAGCTTCTCCGACTATTTGCTCAGCATACAGATTGAATAAGTATCGTAAAAGGATATAACCCTGATATAACCTTTCCTGATTTTAAACTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:124M27S AS:i:151 XS:i:33 A00126:224:H7HMWDSX3:3:1103:12463:35008 145 Test_seq 137484 60 124M27S = 137147 -461 GCTTTCAGCCAAGATCCATCTGACATCAGCAGTGACCTCCCTCATTCCACGTTCTCTTCTAAACCCGGCTTGAATTTCTGGCAGTTCCCTGTTGATGTACCGCTGTAACATTTTTTGAACGATCGATCATTTATCTAAAATTATCTTTATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:124 MC:Z:151M AS:i:124 XS:i:32 A00126:224:H7HMWDSX3:3:1103:9697:35008 97 Test_seq 167173 46 151M = 167508 486 GATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGATTCATGTAAATTTTAGAGATCAGGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF:FFFFFFFFFF:F,FFF,,F,FF:FFFFFFFF:FFFFF,F:FFFF:FFFFFFFFFF:FF:FFF NM:i:5 MD:Z:5A64T55A7G15A0 MC:Z:151M AS:i:130 XS:i:106 XA:Z:Test_seq,-213711,151M,9; A00126:224:H7HMWDSX3:3:1103:9697:35008 145 Test_seq 167508 60 151M = 167173 -486 GGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCAT FFFFF:FFFFFFFF:F:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFF NM:i:1 MD:Z:136T14 MC:Z:151M AS:i:146 XS:i:114 A00126:224:H7HMWDSX3:3:1103:4282:36057 81 Test_seq 169316 60 151M = 169000 -467 TAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAAGTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGG FFFFFFFFFFFFFF::FFFFFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:36T114 MC:Z:27S124M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1103:4282:36057 161 Test_seq 169000 53 27S124M = 169316 467 AACTCCACATAAGGCAGATATTATAACTCTTCTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTA FFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:F:FFFFFFFFFFFFFFFFF:FFFFFFFFFF,F:F:::FFFFFFF,:F,FFFFF,,FFFF:FFFFFFF NM:i:4 MD:Z:11G29T8A14A58 MC:Z:151M AS:i:104 XS:i:78 A00126:224:H7HMWDSX3:3:1103:19768:36573 97 Test_seq 166082 60 151M = 166417 486 CCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,F:FFFFFFFFFFFFFF,FFF:F NM:i:2 MD:Z:27G0A122 MC:Z:151M AS:i:141 XS:i:69 A00126:224:H7HMWDSX3:3:1103:19768:36573 145 Test_seq 166417 60 151M = 166082 -486 TGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCAATAAAAAACCCTC FFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:88T62 MC:Z:151M AS:i:146 XS:i:40 A00126:224:H7HMWDSX3:3:1104:23149:1501 97 Test_seq 170256 60 151M = 170511 394 CTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTT FF:F,,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFF:FF,FFFFFFFFF,FFFFFFFFFFFF:FFFFFFF,F:,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:F NM:i:1 MD:Z:6A144 MC:Z:87M12I52M AS:i:146 XS:i:79 A00126:224:H7HMWDSX3:3:1104:23149:1501 145 Test_seq 170511 60 87M12I52M = 170256 -394 TTTTGTGCTGAGACGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTTATGTTAGTTGAGTCGTTACATTTTTCTTGGCTTTTATCTTGAGTTATGGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCC FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:20 MD:Z:27G23A5A10T13G18A3A3T29 MC:Z:151M AS:i:81 XS:i:25 A00126:224:H7HMWDSX3:3:1104:5339:2096 97 Test_seq 169382 60 87M = 169382 87 TAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCCCTAATTTTTATTATTTGTTTTCTTCGGGTGCCTGATGGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:46T25T12G1 MC:Z:87M AS:i:75 XS:i:46 A00126:224:H7HMWDSX3:3:1104:5339:2096 145 Test_seq 169382 60 87M = 169382 -87 TAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT F:FFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:85G1 MC:Z:87M AS:i:85 XS:i:56 A00126:224:H7HMWDSX3:3:1104:25093:3615 81 Test_seq 16333 60 127M24S = 16111 -349 TGTAATCGCCATTTATATTGGTGAAAGAAGGTATTTTCAATGAATAAGTTTTTGGTCTTGCAAAATTCTATCATTCAATCTTTGGCATTGTTTCTATCACCAAGGCCATATTTTCCAACCACTGATCGATCATCTGTTCATGTGCTTATTA FFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:0 MD:Z:127 MC:Z:151M AS:i:127 XS:i:55 A00126:224:H7HMWDSX3:3:1104:25093:3615 161 Test_seq 16111 60 151M = 16333 349 CTAAATTCAGATGGGATATACTCAAGGTCATATTTTGGCTCTTGTGGACTTGCTCTGATTTTCGTCAGTATCAGCGTGAACTTGCATATGAGTAATTGATGGTCTGTTCCACAGTCGGCCCCTAGCCTTGTTTTGACTGATGATACCGAGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:127M24S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1104:32714:5165 97 Test_seq 238200 60 151M = 238527 478 ATGGTTGAGGGAGCTTGTGAGGGTGAGCACTGGGGGGAGGACACGGTAGGTCACCAAGCACAGAAAGCACCCTTCATTTTTGTAAAAATATGGTAATCTTTTGTTGTATTAAGTCACTGAGCTGTCGGGATTGTTACTGCAGCACAACCTC FFFFFFFFFFF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FF:,,FFFFFFFFFFF::FFF,,FF,F:FF::FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1104:32714:5165 145 Test_seq 238527 60 151M = 238200 -478 TACAACGTCAATTTTATAACTGCATAGGAATAATAACCTATTATTTGGATGTGCCACGCATTACTCAACAGTTCCTCATTGTTAGACCTTTATTTTTCCTGTTTTTCATGTTGTGATTAACATTGTTGATGATAATCTTTGCATACGTTCT :FF,FFFF:F,FFFF:F:FFFFFF,FFFFFFFFF::FFFF,F,F,FFFFFFFFFFFFFFF:FFFFF:FFFF,FFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFF::FF:F:FFFFF:FFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1104:15076:6026 97 Test_seq 171233 60 151M = 171528 446 TCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATCTCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTT FF,FFFFFFFFFFF:FFFFFFFFFFFF:F:FFFFF,FFFFFF:,FFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFF:F:FF:F,FFFFFFFF,F,FF NM:i:1 MD:Z:93A57 MC:Z:151M AS:i:146 XS:i:38 A00126:224:H7HMWDSX3:3:1104:15076:6026 145 Test_seq 171528 60 151M = 171233 -446 TGAGTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTATATGCTGTCTCTCTATGGATTCTTGCAACTTATT FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF,FF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:F NM:i:5 MD:Z:3T16A34T9T51C33 MC:Z:151M AS:i:127 XS:i:60 A00126:224:H7HMWDSX3:3:1104:6325:6997 81 Test_seq 171063 60 151M = 170855 -359 GATATATGATCCTTGGTTGGCAGTTTTTCTCGTTCAGTGCTCTGTATACGTCGTCTCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAATCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCTCTGGCTGC FFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:28A2C23C35G50C8 MC:Z:151M AS:i:126 XS:i:58 A00126:224:H7HMWDSX3:3:1104:6325:6997 161 Test_seq 170855 60 151M = 171063 359 TGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACT FFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFF,FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:F:FFFFFF:FFFFF NM:i:1 MD:Z:104A46 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1104:21658:7279 97 Test_seq 293598 60 32S119M = 293810 363 ACAAAAACATACTGTGAAAACGAGTCTAGAGTAATCCTAAACCTATACTTATGAATGTCATCCTGTCTGGAAATAGAGTTTTCTTTGTTATGTTAATGAGGTCATATCAGTGCCCTAAACCCAAGCTTTTGTGAGTTACAAGGAGAAGCAT FFFFF::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FF,FFFFFFFFFFFFF,FFFF::FFFFFFFFFFF,FFFF::FFFFFFFFFFFFFFFFFFF:,FF,FFF:FFFFFFFF,FFFF:F,FFF NM:i:0 MD:Z:119 MC:Z:151M AS:i:119 XS:i:0 A00126:224:H7HMWDSX3:3:1104:21658:7279 145 Test_seq 293810 60 151M = 293598 -363 GAAAAAGACCAGAATCCTCCTCCACTGTCAAAAAGGAGCCTTCCCCTGGAGCAGGAGCCCTGAATTAGGACTTCCAGCCTCCAGACCAGTGAGAAAATAAATTTCTCTTCTTTAAAGCCCCCACTTGCAGTATTTCTGTTACAGCAGCACT FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFF:FFFFFFF,FFFFFF::FFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:85A65 MC:Z:32S119M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1104:8169:7498 97 Test_seq 167315 8 27S124M = 167558 394 GCCCTCCTGTGGCCAGAAAAATCTCTCAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF::FFFFF:,FFFFFFFFFFF,FFFFFFFFFFFFFF,F NM:i:2 MD:Z:8A44A70 MC:Z:151M AS:i:114 XS:i:109 XA:Z:Test_seq,-213596,124M27S,3;Test_seq,+400736,27S124M,5; A00126:224:H7HMWDSX3:3:1104:8169:7498 145 Test_seq 167558 31 151M = 167315 -394 TTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCCAATTGACTGACACTGGTCCTTTGTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:118A32 MC:Z:27S124M AS:i:146 XS:i:131 XA:Z:Test_seq,+213330,130M2I19M,4; A00126:224:H7HMWDSX3:3:1104:5791:7921 81 Test_seq 171144 60 151M = 171069 -226 CTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGTTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:66C84 MC:Z:24S127M AS:i:146 XS:i:55 A00126:224:H7HMWDSX3:3:1104:5791:7921 161 Test_seq 171069 60 24S127M = 171144 226 ACTATTCTAGCTATCAGCAAGGATTGATCTTTGGTTGGCAGTTTTTCTCGTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTT FFFFFFFFFFFFFFFFFFFFFFF:FFFF::FFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:5C16A2C101 MC:Z:151M AS:i:112 XS:i:68 A00126:224:H7HMWDSX3:3:1104:11568:9377 97 Test_seq 168405 60 151M = 116047 -52337 TATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,:FF:FFFFFFF NM:i:1 MD:Z:90T60 MC:Z:128S23M AS:i:146 XS:i:81 A00126:224:H7HMWDSX3:3:1104:11568:9377 145 Test_seq 116047 21 128S23M = 168405 52337 CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCACAAAATGGAGGACAACTACA FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF NM:i:0 MD:Z:23 MC:Z:151M AS:i:23 XS:i:19 SA:Z:Test_seq,168651,-,126M25S,60,1; XA:Z:Test_seq,-85506,127S19M5S,0; A00126:224:H7HMWDSX3:3:1104:11568:9377 2193 Test_seq 168651 60 126M25H = 168405 -372 CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATC FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:65T60 MC:Z:151M AS:i:121 XS:i:55 SA:Z:Test_seq,116047,-,128S23M,21,0; A00126:224:H7HMWDSX3:3:1104:24921:11741 113 Test_seq 212650 24 151M = 168452 -44199 GCAAGGAAGATTTAAAGTTATCACTTTTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCTGGTTATAAAATAAACATACAAAAATCACTTGGATT F,,,,F,,FF,,:FFF,F,FF,:::,FFF,:FF,FFFFFFF:FFFFF:FF,:F::,F,:FF,,:,:,:FF::FFF,:F:F,FF,:F:F,:::F,,:,FF,:FFF:FFF::,FFFFF,FFF:F,:FFFFF:FFF:F,FFF,FFF,FFF:FFF NM:i:11 MD:Z:7G2A0G4A5T2A17C0T17T52A8G26 MC:Z:151M AS:i:100 XS:i:85 XA:Z:Test_seq,+168238,151M,14; A00126:224:H7HMWDSX3:3:1104:24921:11741 177 Test_seq 168452 60 151M = 212650 44199 TGTTGAATAAGAGCAGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTAT F:FFFFFFFFFF,FFFFFFF,,:,:,FFFFFFFFFFF,:FF:FFFFFFFFFFF:FF:FFFFFFFFF::FFFF:F:FFFFFFFFFFFFFFFFFF:FFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF::F:FFFF:FFFF NM:i:1 MD:Z:14G136 MC:Z:151M AS:i:146 XS:i:93 A00126:224:H7HMWDSX3:3:1104:8323:13463 81 Test_seq 170636 60 151M = 170316 -471 CTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTTTGATCGTTTATCTATTGATTTCCATGATT FFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:3A42G2G3A2A38G25G29 MC:Z:151M AS:i:117 XS:i:23 A00126:224:H7HMWDSX3:3:1104:8323:13463 161 Test_seq 170316 60 151M = 170636 471 AAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAGTAAGTTTTTAGTGCTGTCATTTTGATG FFFFFFFFFFFFFFFFFF::FFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFF:FF:FFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFF,FF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF:FFFF:FFFFFFFF,FFF NM:i:1 MD:Z:123A27 MC:Z:151M AS:i:146 XS:i:52 A00126:224:H7HMWDSX3:3:1104:18855:14418 81 Test_seq 171293 60 129M22S = 171064 -358 TTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTACAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTACTCATGGAGACCCCATGTTAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:81T25G15T5 MC:Z:151M AS:i:114 XS:i:0 A00126:224:H7HMWDSX3:3:1104:18855:14418 161 Test_seq 171064 60 151M = 171293 358 ATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFF NM:i:1 MD:Z:27A123 MC:Z:129M22S AS:i:146 XS:i:73 A00126:224:H7HMWDSX3:3:1104:5828:14622 81 Test_seq 156003 60 136M15S = 155716 -423 TCCCTGATTTCCTTCCTGATGTCTTGAAGGGTTCTGTATATTAATCTTTTGTATTCTGTATCTGATAATTCCAGGAATGCACTTTCATCTAGAAGATCCCTAGATTTGTTGTTTTGAGAGCTTGTTGAGGTGATCACTCCAGGACCTTTGC F:,FF,F,:FF:,F:FFFFFFF:FFFFFFFFFFFFFF,:,F,F:,FFFFFF,,FF:FFF:FFFF,FFFF::FFFF,FFFFFFFFFFFFFF,:F:FFFFFFFFFFFFFFFFFF:FF:FFFFFFF:FFFFFFFFFFF:F::F:FFFFFFFFFF NM:i:2 MD:Z:5C38A91 MC:Z:151M AS:i:126 XS:i:62 A00126:224:H7HMWDSX3:3:1104:5828:14622 161 Test_seq 155716 60 151M = 156003 423 TTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTCTTCAAATGTATTAGGGCCAAGTGATTTATGTTCAATTTCAGAAATTCTAGCTTCTACTTGCTCAATTCTGCTCCTCTGACTTTCTATTGAGTTGTCTACTTCTGTAATTTTATT :,:F:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF,FFFF,FFFFFFFFF,FFFFFF,FF:FFFFFFFF:FFFFFFF,,FFF,FFFFF:FFFFFFF,FF:FF:F NM:i:0 MD:Z:151 MC:Z:136M15S AS:i:151 XS:i:97 A00126:224:H7HMWDSX3:3:1104:5719:17409 113 Test_seq 167167 52 151M = 213899 46733 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA F:FFFFFFF:F,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:76T74 MC:Z:151M AS:i:146 XS:i:121 XA:Z:Test_seq,+213717,151M,6; A00126:224:H7HMWDSX3:3:1104:5719:17409 177 Test_seq 213899 14 151M = 167167 -46733 AGATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACAGAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTATC FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:14T3A26C33C9T54T3T2 MC:Z:151M AS:i:119 XS:i:111 XA:Z:Test_seq,+166985,151M,8;Test_seq,+400413,7S144M,9; A00126:224:H7HMWDSX3:3:1104:27796:17879 81 Test_seq 168961 60 151M = 168874 -238 TCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTAT FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:80T70 MC:Z:151M AS:i:146 XS:i:81 A00126:224:H7HMWDSX3:3:1104:27796:17879 161 Test_seq 168874 60 151M = 168961 238 TTTTCTTTTTTTGTAATGTTTTTACCTGGTTTTGGTATCAGGGAGATGGTAGCTTCATAGAATGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFF NM:i:4 MD:Z:19C20A9G34G65 MC:Z:151M AS:i:131 XS:i:71 A00126:224:H7HMWDSX3:3:1104:10926:18349 81 Test_seq 171045 60 132M19S = 170851 -326 TCAGAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGATTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTAATCTCTCATGTAACCTTT FFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:2C43A40C0C43 MC:Z:151M AS:i:114 XS:i:65 A00126:224:H7HMWDSX3:3:1104:10926:18349 161 Test_seq 170851 60 151M = 171045 326 TTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTAAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFF,FF:F:F NM:i:0 MD:Z:151 MC:Z:132M19S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1104:7075:20478 97 Test_seq 171148 60 151M = 171293 274 TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCATTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF NM:i:1 MD:Z:66T84 MC:Z:129M22S AS:i:146 XS:i:51 A00126:224:H7HMWDSX3:3:1104:7075:20478 145 Test_seq 171293 60 129M22S = 171148 -274 TTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCATCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTACTCCAGCTGTAGTTGATAGCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:41G65G15T5 MC:Z:151M AS:i:114 XS:i:48 A00126:224:H7HMWDSX3:3:1104:7229:20525 97 Test_seq 171148 60 151M = 171293 274 TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCATTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAAT FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF NM:i:1 MD:Z:66T84 MC:Z:129M22S AS:i:146 XS:i:51 A00126:224:H7HMWDSX3:3:1104:7229:20525 145 Test_seq 171293 60 129M22S = 171148 -274 TTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCATCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTACTCCAGCTGTAGTTGATAGCA FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:41G65G15T5 MC:Z:151M AS:i:114 XS:i:48 A00126:224:H7HMWDSX3:3:1104:27416:21355 81 Test_seq 167350 7 151M = 167173 -328 ATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGG FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:18A132 MC:Z:23S128M AS:i:146 XS:i:141 XA:Z:Test_seq,+213534,151M,2; A00126:224:H7HMWDSX3:3:1104:27416:21355 161 Test_seq 167173 43 23S128M = 167350 328 CCCTAATGTAATCCCTAACCTTAGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCCTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFF,,F:FFFFF:FFFFFF:FF,FFFF,F NM:i:2 MD:Z:47T22T57 MC:Z:151M AS:i:118 XS:i:98 XA:Z:Test_seq,-213734,128M23S,6; A00126:224:H7HMWDSX3:3:1104:28411:22075 65 Test_seq 214751 27 37M114S = 203500 -11252 CTAAGATGCATCAATTGCTCTCAACCCACCTGGATCACTCTTTTCCCACCCAAGAACTTTTAAAAACTAAGGCCCATCTTTTCTTCTGTGAAACAGGGGTAAGCACTGCTCTAGGCCCTCCTCGTCTCCACTTGCAAGCCTCTCCAATAAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFF NM:i:0 MD:Z:37 MC:Z:151M AS:i:37 XS:i:32 SA:Z:Test_seq,203705,-,115M36S,60,0; XA:Z:Test_seq,-399667,114S37M,1; A00126:224:H7HMWDSX3:3:1104:28411:22075 2129 Test_seq 203705 60 115M36H = 203500 -320 TTTATTGGAGAGGCTTGCAAGTGGAGACGAGGAGGGCCTAGAGCAGTGCTTACCCCTGTTTCACAGAAGAAAAGATGGGCCTTAGTTTTTAAAAGTTCTTGGGTGGGAAAAGAGT FFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:115 MC:Z:151M AS:i:115 XS:i:0 SA:Z:Test_seq,214751,+,37M114S,27,0; A00126:224:H7HMWDSX3:3:1104:28411:22075 129 Test_seq 203500 60 151M = 214751 11252 GATCCTGTTTTCTGTCTCTCTCTCTCTCTCTGTGTGTATGTGTGTGTGTTTTCCCGAGAAATTACCAATTAGTAAGATTTTCAGAATTTAGTTTGGTATTTATTTGTTTTTGTTTTTTATTTGTAATGTAACCGGAATAAGGTTCTTGCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFF,FFF,F:,::FFFFF,FFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:37M114S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1104:19804:22701 81 Test_seq 167974 60 68M = 167974 -68 GGAAGTGCGTTATATGTATAAATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTAT :FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFFFF NM:i:1 MD:Z:20G47 MC:Z:68M AS:i:63 XS:i:42 A00126:224:H7HMWDSX3:3:1104:19804:22701 161 Test_seq 167974 12 68M = 167974 68 GGAAGTGCATTGCATGTGTAGATGACTTTTGGTAGAATAGATATTTTTACTATGTTAAGTCTTCCTAT FFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:8G2A0T4A6G16C26 MC:Z:68M AS:i:40 XS:i:33 XA:Z:Test_seq,-212997,43M25S,2; A00126:224:H7HMWDSX3:3:1104:28718:23547 97 Test_seq 333499 11 79M = 15135 -318287 TCCACACAGTTGAATGCCTTTGCATAGTCAATAAAACACAGGTAAACATCCTTCTGGTATTCTCTGCTTTCAGCCAAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:76G2 MC:Z:79M AS:i:76 XS:i:71 XA:Z:Test_seq,-383985,79M,2; A00126:224:H7HMWDSX3:3:1104:28718:23547 145 Test_seq 15135 6 79M = 333499 318287 TCCACACAGTCAAATGCCTTTGCATAGTCAATAAAACACAGGTAAACATCTTTTTGGTATTTTCTGCTTTCAGCCAAGA FFFFFFFFFF,,FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:9G40C2C22G2 MC:Z:79M AS:i:61 XS:i:56 XA:Z:Test_seq,+383985,79M,5;Test_seq,-184559,79M,5;Test_seq,-395912,79M,5;Test_seq,-47050,79M,6;Test_seq,-333499,79M,6; A00126:224:H7HMWDSX3:3:1104:21025:24878 113 Test_seq 213589 7 130M21S = 167520 -46049 ACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATATCCAGCTATGACATCTCCGACCAGCGCCTGATCGATCACCTTTCCTGGTAATAG FFFFFFFFFFFFFF:FFFFF:FFF:FFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF NM:i:3 MD:Z:80T15T1T31 MC:Z:151M AS:i:115 XS:i:110 XA:Z:Test_seq,+167316,21S130M,4;Test_seq,+400737,21S123M7S,5; A00126:224:H7HMWDSX3:3:1104:21025:24878 177 Test_seq 167520 54 151M = 213589 46049 TTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGA FFFFFF,:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:130M21S AS:i:151 XS:i:126 XA:Z:Test_seq,+213368,92M2I23M2I32M,5; A00126:224:H7HMWDSX3:3:1104:21576:26490 97 Test_seq 171453 60 24S127M = 171716 414 GATCTAACTGGAAGCAAATAGATCAGTCCCACATAATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTAGTGTTGATTCCCTGGTCCTCCAGATGTCCCAGTCTGCATTCTAATTGCTCGAGTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:F,:FFFFFFFFFFFFFFFFFFFF,,FFF:F:F:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFF NM:i:5 MD:Z:10G59G1A22A10A20 MC:Z:151M AS:i:102 XS:i:38 A00126:224:H7HMWDSX3:3:1104:21576:26490 145 Test_seq 171716 60 151M = 171453 -414 TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATT FFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:1T149 MC:Z:24S127M AS:i:149 XS:i:30 A00126:224:H7HMWDSX3:3:1104:20826:26913 97 Test_seq 171453 60 24S127M = 171716 414 GATCTAACTGGAAGCAAATAGATCAGTCCCACATAATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTAGTGTTGATTCCCTGGTCCTCCAGATGTCCCAGTCTGCATTCTAATTGCTCGAGTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:10G59G1A22A10A20 MC:Z:151M AS:i:102 XS:i:38 A00126:224:H7HMWDSX3:3:1104:20826:26913 145 Test_seq 171716 60 151M = 171453 -414 TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATT FFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:1 MD:Z:1T149 MC:Z:24S127M AS:i:149 XS:i:30 A00126:224:H7HMWDSX3:3:1104:9453:27070 97 Test_seq 169139 60 151M = 169297 309 GTTTAGGTAAGTAGTATTGTTCTAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTATGTAGTAATCTGATATGATTCTTTTGATTTCATTTGGTTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGT F:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFF:FFFFF:FFF,FFFFFFFFFF,FFFF:F:FF:FFFFF,:FF,FFFFFFF:F:FF,FFFFF:FF:::F:FFFFF:FFFFF,F,FFF::F:FF,F,,FF,:F,FFFF NM:i:11 MD:Z:9G5G2T3G7C31C12C12A11A11C16T21 MC:Z:151M AS:i:96 XS:i:58 A00126:224:H7HMWDSX3:3:1104:9453:27070 145 Test_seq 169297 60 151M = 169139 -309 TTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTT ,:FFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::::FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFF:FFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:101 A00126:224:H7HMWDSX3:3:1104:31087:28087 113 Test_seq 167525 52 151M = 213590 46044 CTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTCTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATC FFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF NM:i:1 MD:Z:65G85 MC:Z:129M22S AS:i:146 XS:i:121 XA:Z:Test_seq,+213363,97M2I23M2I27M,6; A00126:224:H7HMWDSX3:3:1104:31087:28087 177 Test_seq 213590 32 129M22S = 167525 -46044 CCAGATAACTGGGAGCTCCTAAAAATGAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATTTTCAGCTATGACATCTCCGACCAGCGCCTGATCGATCCTTGTACTGGTCAGTGTG FF,FF:F:FFFFFFFFFFFFFF:,:FFF,FF:FFF,,FFFFFFFFF:FFFFFFF:FFF,,,FFF,FFF:FFFFFFFFF,F:FFF:FFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFF:F:FFFFFFF,,FF,F,FFFFFFFFFF NM:i:2 MD:Z:26C52T49 MC:Z:151M AS:i:119 XS:i:104 XA:Z:Test_seq,+167316,22S129M,5;Test_seq,+400737,22S123M6S,4; A00126:224:H7HMWDSX3:3:1104:7699:31798 97 Test_seq 170144 60 25S126M = 170398 405 TTTTGTGAACCATTATCCCATGATCGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTT FF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF::F,:F,F:F,FFF:FFFFFFF:FFFFFF:::F: NM:i:1 MD:Z:52A73 MC:Z:151M AS:i:121 XS:i:49 A00126:224:H7HMWDSX3:3:1104:7699:31798 145 Test_seq 170398 60 151M = 170144 -405 GGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:92T58 MC:Z:25S126M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1104:22869:31923 65 Test_seq 171253 9 36M115S = 14083 -157171 GATGATAATATGTCTTGGTGATTTCCTTTTGGGATCGATCAAGTAGACACATGAGACTATGTTAGCATCTCCTGTCTGGAGGGGAGATGAGAGAGCAGAGGGGTCAGAACCTGGTGGAACGGACATGAAAATAGAGAGTGGAGGGAAGGAG FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:20T3T5T5 MC:Z:151M AS:i:21 XS:i:0 SA:Z:Test_seq,14276,-,115M36S,60,0; A00126:224:H7HMWDSX3:3:1104:22869:31923 2129 Test_seq 14276 60 115M36H = 14083 -308 CTCCTTCCCTCCACTCTCTATTTTCATGTCCGTTCCACCAGGTTCTGACCCCTCTGCTCTCTCATCTCCCCTCCAGACAGGAGATGCTAACATAGTCTCATGTGTCTACTTGATC FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFF NM:i:0 MD:Z:115 MC:Z:151M AS:i:115 XS:i:42 SA:Z:Test_seq,171253,+,36M115S,9,3; A00126:224:H7HMWDSX3:3:1104:22869:31923 129 Test_seq 14083 60 151M = 171253 157171 TGAGCAATATTTTCCTCTCCCCCTCCCTCCTGCCACTGGTTACCACCTATAAAGTTTGGTGTCTATATATTTGCCTTTTCTTTTTTATTGTGCTTTATGTAAAAGTTTACAAACCAAGTCTATCTCTCATACAAAAATTTATATACATCTT FFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFF:F:FFFF:,FFFFFFFFFFFFFFFFF:FFFFFF:FF:FFFFF:F, NM:i:0 MD:Z:151 MC:Z:36M115S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1104:24505:33880 97 Test_seq 168347 60 29S122M = 168573 377 CCTTGAGGATGTCCCCTGGGGGAGATTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACGCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCAGT FFFFFFFFFFF:FFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:18A35C64G2 MC:Z:151M AS:i:109 XS:i:59 A00126:224:H7HMWDSX3:3:1104:24505:33880 145 Test_seq 168573 60 151M = 168347 -377 TATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATG FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:143T7 MC:Z:29S122M AS:i:146 XS:i:71 A00126:224:H7HMWDSX3:3:1104:25147:34115 81 Test_seq 169628 60 151M = 169296 -483 CGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCTTTGTGGTCTGA FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:4 MD:Z:0T34G83C19C11 MC:Z:151M AS:i:135 XS:i:67 A00126:224:H7HMWDSX3:3:1104:25147:34115 161 Test_seq 169296 60 151M = 169628 483 TTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACTAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFF,:FFFFF:FFFFFFFFFFFFF NM:i:1 MD:Z:70C80 MC:Z:151M AS:i:146 XS:i:96 A00126:224:H7HMWDSX3:3:1104:10004:34538 81 Test_seq 214062 60 151M = 213885 -328 AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGATGCAACCAAGGTGTCCATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:1 MD:Z:132A18 MC:Z:14S82M1D55M AS:i:146 XS:i:108 A00126:224:H7HMWDSX3:3:1104:10004:34538 161 Test_seq 213885 27 14S82M1D55M = 214062 328 ACGTGGCCGTGGAGTTAAAACTACGATGAGGTTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACTCAAAATAATAAATGTTGGAGAGGTGCGGAGAGATTGGAACTCTTATACACTGCTGGTAGGAATGGAAAATGGTACAAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:7 MD:Z:6G9A41A23^C10C23G6T13 MC:Z:151M AS:i:100 XS:i:85 XA:Z:Test_seq,-400433,55M1D82M14S,10; A00126:224:H7HMWDSX3:3:1104:31819:35524 97 Test_seq 41510 3 50M = 213837 172377 GATACATGAGGAAATGCTCACGATCATTAGCCATTAGAGAAATGCAAATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:19T29C0 MC:Z:50M AS:i:44 XS:i:41 XA:Z:Test_seq,-49114,4S46M,1;Test_seq,+213837,50M,2;Test_seq,-167148,50M,2;Test_seq,+258764,50M,3; A00126:224:H7HMWDSX3:3:1104:31819:35524 145 Test_seq 213837 0 50M = 41510 -172377 GATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATT FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:50 MC:Z:50M AS:i:50 XS:i:50 XA:Z:Test_seq,+167148,50M,0;Test_seq,-41510,50M,2; A00126:224:H7HMWDSX3:3:1104:31512:35556 97 Test_seq 167362 21 151M = 167596 385 TCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:6A144 MC:Z:151M AS:i:146 XS:i:136 XA:Z:Test_seq,-213528,6S145M,2; A00126:224:H7HMWDSX3:3:1104:31512:35556 145 Test_seq 167596 7 151M = 167362 -385 GGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCCAATTAACTGACACTGGTCCTTTGTCAAATATCAGCTGCTCATACATGGATGGATTTATATCTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:80A11G37T20 MC:Z:151M AS:i:136 XS:i:131 XA:Z:Test_seq,+213292,151M,4;Test_seq,+40961,151M,5; A00126:224:H7HMWDSX3:3:1104:25093:35556 97 Test_seq 290555 60 151M = 290866 462 CCTAGGCAAGATTTTTGCCTTCAGACCTCAGCAGCCAAAATGTTCTCAATGGGAGAGTGCAATTCTAGACACGAGAGCTTGGTTCCAAGAAAGACTGGAAGTCCTCTTGGTACTGGAGACCTCAGGGAAAGGCTGCTTGCAGGTGTCCCAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,F:F:FF:FFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1104:25093:35556 145 Test_seq 290866 60 151M = 290555 -462 AAACCCAAGAGATTGGCTCATCCATGCACTGTGAAGAATGTGTAATGGCTCCCATGATGCTTCATCTGTTCTGGGATACAACAGCAGTAACGATACTAAAAATAAAACTTTGTTTTTTCCCAGCGTTATTACATTTTACAAAGTTATATTT FFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FF:FFFF,F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1104:6126:35744 81 Test_seq 168443 60 151M = 168347 -247 CAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTC F,FFF:F:FFFFFF:FFFFFFF:FFFFFFFFFFFF:FFFFFFFF,FF:FF:F,FFFFFF,FFF:::FF:FFF:::FFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFF,FFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFF: NM:i:1 MD:Z:52T98 MC:Z:19S36M1I95M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1104:6126:35744 161 Test_seq 168347 60 19S36M1I95M = 168443 247 AAGTCAGTGGGGGGAGTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGG FFFFFFFFFFFFFFFFFFFF,F:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FF::FFFFFFFF::FFFFFFFFFF,FFFF::FF:FFFFFFFFFFF:FFFF:F:FFFFFFFFFF:FFFFFF:FFFF: NM:i:3 MD:Z:18A21G90 MC:Z:151M AS:i:114 XS:i:56 A00126:224:H7HMWDSX3:3:1104:21314:35994 97 Test_seq 166983 34 151M = 167183 351 AAGATAGCGCCAGATGGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCATCAGTGTATGAGAGTTCCAATCTCTCCGCACCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGTCTAGTTGGTGTGAGATGGTAT FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:F:::F:,F:FFFFFF:FF:FFFFFF:FFFFFF,F,::FFFFFFFFFFFFF:F:,FF NM:i:7 MD:Z:6A38A8G18G7A2G63A2 MC:Z:151M AS:i:118 XS:i:99 XA:Z:Test_seq,-213901,142M9S,9; A00126:224:H7HMWDSX3:3:1104:21314:35994 145 Test_seq 167183 60 151M = 166983 -351 ATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGAGCTGGTCTGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:60T90 MC:Z:151M AS:i:146 XS:i:113 A00126:224:H7HMWDSX3:3:1105:4218:2722 97 Test_seq 126403 60 19S132M = 126723 471 CCCACAACTCCTATAGATCAATCAACTCGACAGCAACAGATTTGGTTTTTAGAAACCTACACAGCTGAGCTCTGGATTGGGACAATCAAAATAGCAATTCCCTGGGCCTCAGCTCTCTCCCAGAAAGTAATTAATGCCATGGATATGGCTT FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FFFFFFFFFFFFFF NM:i:0 MD:Z:132 MC:Z:151M AS:i:132 XS:i:0 A00126:224:H7HMWDSX3:3:1105:4218:2722 145 Test_seq 126723 60 151M = 126403 -471 GGATCCTCGGGCTCCCAAGGAGGCTGTGTGGCTAGAACGTAAGAAGTCTGTGAACTTAGATGGGAAAAAAAAAGTACATATTTATTTGTGCTGACTTCTAACTGAAAATTAGTATACCCATCAGTAGCGACTGTCAGCTGTGACTTTGTCC FFFFFFFFFFFF:FFF,FFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFF,F:F,FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:19S132M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1105:25699:3129 81 Test_seq 171784 60 151M = 171489 -446 TGTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGT FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:1T149 MC:Z:16M1D135M AS:i:149 XS:i:21 A00126:224:H7HMWDSX3:3:1105:25699:3129 161 Test_seq 171489 60 16M1D135M = 171784 446 TAATTCTTTTATCTGATTTTTTTCAGCTATGTTGGTGGTGTTTTCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATT FFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:8 MD:Z:16^T19A1T2A2C49T9T13T33 MC:Z:151M AS:i:109 XS:i:32 A00126:224:H7HMWDSX3:3:1105:6090:3865 97 Test_seq 213577 18 151M = 213778 352 TGATGAAGAGAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATTTTCAGCTATGACATCTCCGACTAGCACCTGATCTCTAAAATC FFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:0C91T37C3G16 MC:Z:151M AS:i:135 XS:i:126 XA:Z:Test_seq,-167307,151M,5;Test_seq,-400728,132M19S,3; A00126:224:H7HMWDSX3:3:1105:6090:3865 145 Test_seq 213778 60 151M = 213577 -352 GAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAAGTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCA FFFFF::FF,FFFFFFFFFFF:FF,,:,F:,FFFFFFFFF:FFFF,FFF:FFFFFFFFFFFFFFFFFF,FFFFF:FFFF:FFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FF:FFFFFFFF::FFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:106 A00126:224:H7HMWDSX3:3:1105:5593:4758 97 Test_seq 310384 60 151M = 310568 335 GATCTACAGCTCATCAGGGAGAGTAGGAGTTAGGGACGGGGCTTAGGAAGCCAGGGACAAGGTGAAGTTCTGGCTGTGCATGAAATTGTCAAAGAGGAACATGAAAAGAGAAGGACAAAGGACTAAAGCTCAAGCAGAAAAATAAAGTGGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1105:5593:4758 145 Test_seq 310568 60 151M = 310384 -335 CTAAATATCTACAATACCTGCTTTATTGTTACAGTTGCGTACCATCTAGTTGTCTCCAACTCATAGCAACCCTATAGGACAGCAGAACTGCCCCACAGGGTTTTCTAGGCTGAAATCTTTATGGGAGCAGATCACTAGGTCTTTCTCCTGA FFFFFF,:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:28 A00126:224:H7HMWDSX3:3:1105:8359:6292 81 Test_seq 357118 60 151M = 356837 -432 AAAACCACAAAATAATAAATGTTAGAGAGGTTGTGGAGAGACTGGAATACTTAGGGGCAGCTGGTGGGAATGTAAAATGGTACAGTCACTTTGGAAGTTGATTTGGCACTTATTTAAAAAGCTAGGAATAGAACTACCATAATATCCAGGA FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:31 A00126:224:H7HMWDSX3:3:1105:8359:6292 161 Test_seq 356837 60 151M = 357118 432 ATAATGTCATGTAGACTTTATCATCTCAAATGACACCAATATTTTATACTTTTAAGTCAAAAGTTAAACTTTTAAATGCCCATTTTGACTTGAGATCTTCAATCCTTTAATTCAATACTGTTAGAATAAATGTTTAAAATGCAGAATTTAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1105:11776:6480 81 Test_seq 168752 60 151M = 168519 -384 TACCTGGTATAAATCCCACTTGATCATGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:26G124 MC:Z:49M1D102M AS:i:146 XS:i:83 A00126:224:H7HMWDSX3:3:1105:11776:6480 161 Test_seq 168519 60 49M1D102M = 168752 384 GTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFF:FFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:49^T25A76 MC:Z:151M AS:i:139 XS:i:79 A00126:224:H7HMWDSX3:3:1105:7862:8343 81 Test_seq 258273 60 151M = 258022 -402 GGGAAAAGACAGTCTCTTTAACAAATGGTGCTGGCACAACTGGATATCCATCAGCAAAAAAATGCAACAAGACTCATACTTCACACCATGCACAAAAACGAACTCAAAATGAATCAAAGACCTAAATATAAAATCTAAAACAATAAAGATC F,:FFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFF:::FF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:20S131M AS:i:151 XS:i:66 A00126:224:H7HMWDSX3:3:1105:7862:8343 161 Test_seq 258022 60 20S131M = 258273 402 ATGCCTGCACAGCAGTCTCTCAGATCACCAACTTCATATGGAAAGGGAAGCATCTCTGGATAAGTAAATCATTACTGAAAAAGAAGAACAAAGTGAAAGGCCTCACACTGTCTGATTTTAGAACCTATTATAATGCCACAGTAGTCAAAAC FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:0 MD:Z:131 MC:Z:151M AS:i:131 XS:i:32 A00126:224:H7HMWDSX3:3:1105:24858:9220 97 Test_seq 56335 60 151M = 56613 425 CAAAGAAGAAGTTGTTGGTCTTGCAAAATTCTATCATGCAATCACTGGTGTCATTTCTATCACCAAGGCCGTATTTTCCAACTACCAATCCTTTTTCTTTGTTTCCAACTTTCAAATTCCTATCACCAGTAATTATCAACGCATCATGACT FFF,FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:116M4I31M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1105:24858:9220 145 Test_seq 56613 60 116M4I31M = 56335 -425 TATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCGATCCAAAATGGCCAATATCAGTCCATTGCA FFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF NM:i:4 MD:Z:147 MC:Z:151M AS:i:137 XS:i:84 A00126:224:H7HMWDSX3:3:1105:3079:9267 97 Test_seq 169966 60 151M = 170207 392 GTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGTGGTGTGTTGAAGTCTCCTACTATATATGTGGAGGTGTCTATCTCACTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTAGGTGCGT FFFFFFFFFFFFFFFFFFFFF:FF:FFFF:FFFFFFFFFFFF:FFFFFFFFF::FFFFFFF:F:FFFFF:F:FFFFFFFFF,F,FFFFFFFFFFFFFFFFFFFFF:FFFF,FFFF,FFFFFFFFFFFFFF:FF,F,FFFF::FF,FF:FFF NM:i:7 MD:Z:8G53C9A8A10G48C1G7 MC:Z:151M AS:i:116 XS:i:52 A00126:224:H7HMWDSX3:3:1105:3079:9267 145 Test_seq 170207 60 151M = 169966 -392 TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC :F,FFFFFFF:FFF,FFFF:FFFFFFFFFF,:FFFFFFFF:F,,:FFFFFF:FFF,FFFFFFF,FFFFFFFF,F:FFFFF:FFFFFFF:FF:FFFFFF:F:FFFFFFFFFFF:FFF,,FFFFFFF,FF,FFFF,FFF:FFFFFFFF,FFF, NM:i:1 MD:Z:55A95 MC:Z:151M AS:i:146 XS:i:88 A00126:224:H7HMWDSX3:3:1105:24840:9659 97 Test_seq 170869 60 151M = 171041 308 GAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTA FFFFFFFFFFFFFFFFFFFFFFF:,FFFFFF:F:,FF:FFFFFFFF,F:FFFFFFFFFFFFFFFF,FFFFFFFF:FFFFF:FF::FFF,:FF:FFFFF,:FFF:F,F,FFFF:FFFFFFFF:FFFFF:FFF:FFF,:F:FFFFFFF,F,FF NM:i:1 MD:Z:90A60 MC:Z:136M15S AS:i:146 XS:i:23 A00126:224:H7HMWDSX3:3:1105:24840:9659 145 Test_seq 171041 60 136M15S = 170869 -308 TATTTCAGAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTGTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTAAAGCACCCCCAGGG FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:6C43A23G26T34 MC:Z:151M AS:i:116 XS:i:76 A00126:224:H7HMWDSX3:3:1105:29984:9768 81 Test_seq 169626 60 57M1I93M = 169353 -423 CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCCTTGTGGCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:6 MD:Z:2T11A22G83C26T1 MC:Z:151M AS:i:123 XS:i:65 A00126:224:H7HMWDSX3:3:1105:29984:9768 161 Test_seq 169353 60 151M = 169626 423 TTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFF:FFF:FFFFFFFFFF:,FFFFF,FFFF,FFFFFFFFFFFFF NM:i:1 MD:Z:114G36 MC:Z:57M1I93M AS:i:146 XS:i:101 A00126:224:H7HMWDSX3:3:1105:27724:12399 97 Test_seq 384489 5 52M = 384499 52 ATGGAGAAAAGATTGAAGTTGTCAAGGATTTCATTTTACTTGGATCCACAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:1C50 MC:Z:10S42M AS:i:50 XS:i:47 XA:Z:Test_seq,-333022,52M,1;Test_seq,+204890,3S49M,1; A00126:224:H7HMWDSX3:3:1105:27724:12399 145 Test_seq 384499 4 10S42M = 384489 -52 ATGGAGTGATGATTGAAGTTGTCAAGGATTTCATTTTACTTGGATCCACAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF: NM:i:0 MD:Z:42 MC:Z:52M AS:i:42 XS:i:40 A00126:224:H7HMWDSX3:3:1105:30861:14951 97 Test_seq 171173 60 151M = 171502 456 GATTCTCCTTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAG FFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF: NM:i:1 MD:Z:8C142 MC:Z:127M24S AS:i:146 XS:i:36 A00126:224:H7HMWDSX3:3:1105:30861:14951 145 Test_seq 171502 60 127M24S = 171173 -456 TGATTTTTTTTCAGCTATGTTGGTGTTGTTTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTTCTCGAATCTGCTCCTCTGACTTCCTATTGCATTGTCTAATTCTGTAATTTTATTGTTAGATCTTAATTTGTTTTTGTGATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:23A4A39G5G6T9T7G27 MC:Z:151M AS:i:92 XS:i:25 A00126:224:H7HMWDSX3:3:1105:7419:15436 97 Test_seq 214052 60 151M = 214268 366 AAAAAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTACTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFF NM:i:1 MD:Z:109T41 MC:Z:117M1I33M AS:i:146 XS:i:106 A00126:224:H7HMWDSX3:3:1105:7419:15436 145 Test_seq 214268 36 117M1I33M = 214052 -366 TCGATAAAGAACAGTGACGAATCTGGGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATTAGTCAGAGGCAAAAGGACAAATATTGTATAAGACCACGATTATAAGATCTTGAGAAATAGTATAAACTGAGAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:2A22T45T29C13T34 MC:Z:151M AS:i:120 XS:i:100 XA:Z:Test_seq,+400037,151M,11; A00126:224:H7HMWDSX3:3:1105:23077:15562 81 Test_seq 167359 17 151M = 167131 -379 CAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGG FFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:1 MD:Z:9A141 MC:Z:25S126M AS:i:146 XS:i:138 XA:Z:Test_seq,+213528,3S148M,2; A00126:224:H7HMWDSX3:3:1105:23077:15562 161 Test_seq 167131 22 25S126M = 167359 379 CCTCCACCCTCTTAAAAATATGTTAAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:13C98T13 MC:Z:151M AS:i:116 XS:i:106 XA:Z:Test_seq,-213778,126M25S,4; A00126:224:H7HMWDSX3:3:1105:27923:18443 81 Test_seq 168689 60 122M29S = 168451 -360 CTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAATCTACAGTATGATAAAAAAAAAAGTTA :FFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:27T94 MC:Z:151M AS:i:117 XS:i:54 A00126:224:H7HMWDSX3:3:1105:27923:18443 161 Test_seq 168451 60 151M = 168689 360 ATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA :FFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,:FFF:FFFF,FFFFFFFF:FFFFFF:FF,FFF NM:i:1 MD:Z:44T106 MC:Z:122M29S AS:i:146 XS:i:93 A00126:224:H7HMWDSX3:3:1105:8820:20337 81 Test_seq 171441 60 132M19S = 171233 -340 CCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCGGCTATGTTGGTGTTGATTTCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTTGAGTTAATTTTTCTACTA FFFFFF:FFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:73A10A7C39 MC:Z:151M AS:i:117 XS:i:43 A00126:224:H7HMWDSX3:3:1105:8820:20337 161 Test_seq 171233 60 151M = 171441 340 TCTTTGGTTTTGGCAAGTTTGATGATAATATATCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTT FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFF:FFFFFFFFF,FFFFFFFF NM:i:1 MD:Z:31G119 MC:Z:132M19S AS:i:146 XS:i:36 A00126:224:H7HMWDSX3:3:1105:10276:21010 113 Test_seq 51877 0 132S19M = 171138 119394 AAAAGAAAGGTTTCCTTCAAGGGAGAATCAATAAGAATAAGTTCAGACTACTCAGCAGAAACCATGCAGGCAAGAAGGGAATGGGATGACGTATACAGAGCACTGAAGGAGAAAATCTGCCAACCAAGGATCGATCAAGTTGGTTGATTGT FFFFFF,,:,,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:19 MC:Z:151M AS:i:19 XS:i:0 SA:Z:Test_seq,171070,+,19S132M,60,3; A00126:224:H7HMWDSX3:3:1105:10276:21010 2145 Test_seq 171070 60 19H132M = 171138 219 GATCCTTGGTTGGCAGATTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,,:,,FFFFFF NM:i:3 MD:Z:16T4A23G86 MC:Z:151M AS:i:117 XS:i:74 SA:Z:Test_seq,51877,-,132S19M,0,0; A00126:224:H7HMWDSX3:3:1105:10276:21010 177 Test_seq 171138 60 151M = 51877 -119394 TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTGGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:105T45 MC:Z:132S19M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1105:18837:21089 97 Test_seq 165754 60 151M = 166061 458 AGTCAGCCCTTTCAGTCTCTCGTTTCGTGCCAATTTTGCCGTCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTAATTTAATTAGCTCACTCTTCATCAGCATCTCTCTCCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:3 MD:Z:40A5T75G28 MC:Z:151M AS:i:136 XS:i:0 A00126:224:H7HMWDSX3:3:1105:18837:21089 145 Test_seq 166061 60 151M = 165754 -458 TATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAATTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:82C68 MC:Z:151M AS:i:146 XS:i:75 A00126:224:H7HMWDSX3:3:1105:16640:21167 97 Test_seq 167316 7 22S129M = 167511 346 TGAACCCTGATTGATTTGGATCGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGG FFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFF:FF:FFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFF,FFFFFFFF:FFFFFFFFF:FFFFF NM:i:4 MD:Z:7A37G6A73T2 MC:Z:151M AS:i:111 XS:i:106 XA:Z:Test_seq,-213590,129M22S,5;Test_seq,+400737,22S123M6S,6; A00126:224:H7HMWDSX3:3:1105:16640:21167 145 Test_seq 167511 60 151M = 167316 -346 TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:FFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:22S129M AS:i:151 XS:i:122 XA:Z:Test_seq,+213377,83M2I23M2I37M4S,5; A00126:224:H7HMWDSX3:3:1105:7835:24173 97 Test_seq 171202 60 151M = 171381 329 CTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGCGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:108A42 MC:Z:101M1I49M AS:i:146 XS:i:76 A00126:224:H7HMWDSX3:3:1105:7835:24173 145 Test_seq 171381 60 101M1I49M = 171202 -329 TTTTCTCTGTGTTTTCTGTCCCCTCTTCCTGTTCTGGGACTCCAATCACTCACAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTCCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:8 MD:Z:19G3C2C8T15G46T45A5 MC:Z:151M AS:i:108 XS:i:28 A00126:224:H7HMWDSX3:3:1105:19759:25692 113 Test_seq 167355 7 151M = 213586 46232 TTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCT FFFFFF:F,FFF:FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:1 MD:Z:13A137 MC:Z:151M AS:i:146 XS:i:141 XA:Z:Test_seq,+213529,151M,2; A00126:224:H7HMWDSX3:3:1105:19759:25692 177 Test_seq 213586 19 151M = 167355 -46232 GAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAAAATGGGAAAAAATTTTCAGCTATGACATCTCTGACCAGCGCCTGATCTCTAAAATCTATATGATT FFFFFFFFFFFFFFFFFFFFFFFFF:FF::FFFF:,FFF,FFFFFF,FFFF,FF:F:FFFFFFFFFF:FFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:83T1G1C7G21C33 MC:Z:151M AS:i:126 XS:i:116 XA:Z:Test_seq,+400719,141M10S,5;Test_seq,+167298,151M,8; A00126:224:H7HMWDSX3:3:1105:14091:25864 97 Test_seq 170229 60 151M = 170383 305 TAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTG FFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF::FFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:77 A00126:224:H7HMWDSX3:3:1105:14091:25864 145 Test_seq 170383 60 151M = 170229 -305 CTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGAGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTT FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:96T10T43 MC:Z:151M AS:i:141 XS:i:45 A00126:224:H7HMWDSX3:3:1105:23719:28792 97 Test_seq 167149 42 151M = 167439 423 ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAA FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFF,F,:FFFFFFFFFFF:FFFF:FF NM:i:1 MD:Z:94T56 MC:Z:133M18S AS:i:146 XS:i:126 XA:Z:Test_seq,-213735,151M,5; A00126:224:H7HMWDSX3:3:1105:23719:28792 145 Test_seq 167439 6 133M18S = 167149 -423 ATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGGTCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCAGAACAAACCAGAG F:F:FFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:3T47C22A0T2T15A38 MC:Z:151M AS:i:104 XS:i:99 XA:Z:Test_seq,-400906,4S129M18S,6; A00126:224:H7HMWDSX3:3:1105:8024:29074 97 Test_seq 167861 60 151M = 168146 410 CTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTGGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAAT FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFFFFFF NM:i:1 MD:Z:64A86 MC:Z:125M26S AS:i:146 XS:i:62 A00126:224:H7HMWDSX3:3:1105:8024:29074 145 Test_seq 168146 8 125M26S = 167861 -410 TTATTCCTAAGTATTTTATCTTCTTGGTGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAAGGGGAAGTAAACATCATATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F NM:i:2 MD:Z:23C3G97 MC:Z:151M AS:i:115 XS:i:110 XA:Z:Test_seq,+212763,21S130M,4; A00126:224:H7HMWDSX3:3:1105:24406:30389 81 Test_seq 33778 60 151M = 33624 -305 TCAGCCTGGCCTCTGCCCTGCTTGGGCAAGTGTTACAAAGCTGTTTAGCTCTGCCAGTAAGTGCCCGGAGGCACCCTACTGCACCAATAAGCCTTCTGTCTGAAGGTGTTCAGCTCTCCCACTCCATGGGCCAGCATACCCACTGTAACTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:19S132M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1105:24406:30389 161 Test_seq 33624 60 19S132M = 33778 305 ACTAAGACACATCACGATCGATCAGGAAACATGGGGAAAGTCTCCTTTCTTCAGTACAGGCAGCTCTCTCAGCTCCTTTTGGCTATGCAGGCCTCCTCTCAGCCCCCTCAGGACAGGCTTCCTCTTGACCCCCTCAGGACACACCTCCTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFF,FF NM:i:0 MD:Z:132 MC:Z:151M AS:i:132 XS:i:0 A00126:224:H7HMWDSX3:3:1105:18114:31422 113 Test_seq 167883 60 151M = 213236 45343 TGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGT FFF:F:FFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:140M11S AS:i:151 XS:i:56 A00126:224:H7HMWDSX3:3:1105:18114:31422 177 Test_seq 213236 0 140M11S = 167883 -45343 AGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCACGTATGAGCAGCTGATATTTGACAAAGGACCAGTGTCAGTCAATTGGGGAAATGATAGTCTTTTTATAAGTGCTCCC FFFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFFFFF:FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:74T1A63 MC:Z:151M AS:i:130 XS:i:130 XA:Z:Test_seq,+167663,11S140M,2; A00126:224:H7HMWDSX3:3:1105:14407:31955 97 Test_seq 175119 60 151M = 175457 489 TTTCTCTCCTCAAGGAGAGGAACTAAAAAACATATGTAAACACACACACACACGAGTGTACACAACACAGAAGAGCAATGGCCCACAGCTCAGCTCTTTGATCCTTTCTGAAGCTGTGAGAGAATAGTATTCTTACCTCTTGGCTCATGAA :FF:FFFF,F:FFFFFF:FF:FFFFFFF::FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF::,F::FFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF,FF,F:FFF:F:F:FFF,FFFF,:F,F:FFFFFFFF:FFFFFFF,FFFF NM:i:1 MD:Z:124A26 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1105:14407:31955 145 Test_seq 175457 60 151M = 175119 -489 TTTCAGGTGACATCCTTTCAGATCTGGAAGCTGAGACTCAGTAAGGTGAGTGCCTGGCTCAGCATCCCTCAGCTAACAAGTCCCATAGGACTTGGAACTCCAGATTCTTCACTCTTTTTTCTAATATGCAAATGGTTTTCTGGGTTCCTAA FFFFF,::FF,FF:FFFFFFFFFFFFFF:FFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF,:F,FFFFFF,:FFFF,:FFFF:F:FFFFFFFFFFFFFFFFFFF:FFFF:F:FF,FFF,FFFFFFF:FFFFFFF,FF,FFFFFFFFF:FF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1105:8739:33317 81 Test_seq 167512 60 151M = 167300 -363 GATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGT :FFFFFFFF:FFFF:FFFFFFFFF,FFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:132T18 MC:Z:151M AS:i:146 XS:i:105 A00126:224:H7HMWDSX3:3:1105:8739:33317 161 Test_seq 167300 19 151M = 167512 363 TCATGTAGATTTTAGAGATCAGGCACTGGTCTGAGATGTCATAGCTGAATATTCTTTCGCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTC FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:23A0G33C9A73T8 MC:Z:151M AS:i:126 XS:i:116 XA:Z:Test_seq,-213584,151M,7; A00126:224:H7HMWDSX3:3:1105:29604:34162 113 Test_seq 212615 17 151M = 168475 -44141 CAGGCTAGACAAAGAAATAAAAGGTATCCGGATTGGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF NM:i:10 MD:Z:0T20G2C3T13G8A5T20C0T17T53 MC:Z:151M AS:i:105 XS:i:95 XA:Z:Test_seq,+168273,151M,12; A00126:224:H7HMWDSX3:3:1105:29604:34162 177 Test_seq 168475 60 151M = 212615 44141 GGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAA FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:20T130 MC:Z:151M AS:i:146 XS:i:94 A00126:224:H7HMWDSX3:3:1105:8296:34491 97 Test_seq 168347 34 26S125M = 168526 330 GGTTTAATTTTTCAAAGCGTGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCTCTGGCTAGGACTTCTAGCACAATGTTGAATAAGAGCGGTGAT :FFFFFFFF,FFF,FFFF:FFFFF:FFFF::FFFF:FFFFFFFF:FFF:FFFFFFFFFFFFFFFFFF::FFFFFFFFF::F:,FFFFFFFFFFFFFFFFFF::FFFF:FFFFF:::FFFFFFFF:F:FFFFFFFFFFFFFF,:FFFFFFFF NM:i:6 MD:Z:18A21G41C14A2T2G21 MC:Z:151M AS:i:95 XS:i:77 XA:Z:Test_seq,-212567,127M24S,10; A00126:224:H7HMWDSX3:3:1105:8296:34491 145 Test_seq 168526 60 151M = 168347 -330 CCATTTCGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCA FFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:6A144 MC:Z:26S125M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1105:29441:34538 81 Test_seq 166403 60 151M = 166096 -458 CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGAACTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTAATTGATC FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:49G38C13T40G7 MC:Z:151M AS:i:131 XS:i:37 A00126:224:H7HMWDSX3:3:1105:29441:34538 161 Test_seq 166096 60 151M = 166403 458 TGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCT FFF::FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFF:FFFFFFF:FF:FFFFFF:FFFFFF:FFFFFF:FFFFFFFFF:F:F,FFFFFF:FFFF:FFF::FFFFFFFFFFFFFFFF:FF::F:FFFFFF,F,FF:FF NM:i:1 MD:Z:128G22 MC:Z:151M AS:i:146 XS:i:83 A00126:224:H7HMWDSX3:3:1106:20039:2910 81 Test_seq 316605 60 151M = 316262 -494 TCTAGCCTCATGGCGAAGGAGGCAGTTGTTCACGGTGGCAATTAGCCACACTTTCCATATGGTCCTTCTGCTCCTGACGCTCCTTCTTCCTCTGTTGCTCCAGACAAATAGAGATCAATTGTTGTGCCTTGGGTGACCACTTGCAAGATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:149M2S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1106:20039:2910 161 Test_seq 316262 60 149M2S = 316605 494 GGTAAACATCCTTCTGGTATTCTCTGCTTTCAGCCAGGATCCATCTGACATCAGCAATGATATCCCTGGTTCCATGGCCTCTTCTGAAACCGGCCTGAATTTCTGGCAGTTCCCTGTCAATAAACTGCTGCAGCCGTTTTTGAATGATCAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFF NM:i:0 MD:Z:149 MC:Z:151M AS:i:149 XS:i:119 A00126:224:H7HMWDSX3:3:1106:1651:2942 81 Test_seq 170290 60 151M = 170100 -341 TTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAAT FFFF,:FFFF,FFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,FFFFFFFF,F:F:FFF,FFF,FFFFFFFFFFFF NM:i:1 MD:Z:86C64 MC:Z:151M AS:i:146 XS:i:83 A00126:224:H7HMWDSX3:3:1106:1651:2942 161 Test_seq 170100 57 151M = 170290 341 CCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTATGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTT FFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFF,FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFF::FFFFFFFF,FFFFF:FFFFFF:FFFFFF,,FFF:FFFFFFF:,FFFFF,FFFF:FF NM:i:6 MD:Z:7C7G20A58C0A6A47 MC:Z:151M AS:i:121 XS:i:90 A00126:224:H7HMWDSX3:3:1106:31051:3035 81 Test_seq 168737 60 104M = 168737 -104 TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTT FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:104 MC:Z:104M AS:i:104 XS:i:49 A00126:224:H7HMWDSX3:3:1106:31051:3035 161 Test_seq 168737 60 104M = 168737 104 TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:104 MC:Z:104M AS:i:104 XS:i:49 A00126:224:H7HMWDSX3:3:1106:29053:3709 97 Test_seq 166376 60 15S136M = 166403 178 GCCTCTGGTACTTTTTTAGAAGTCTCCTCAATCCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAACTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTG FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FFFF:FFFFFFFFFFFF::FFF:FFFFFFFFFF:F:FFFFFFFFFFF:FF NM:i:2 MD:Z:16A43G75 MC:Z:151M AS:i:126 XS:i:0 A00126:224:H7HMWDSX3:3:1106:29053:3709 145 Test_seq 166403 60 151M = 166376 -178 CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAACTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:33G117 MC:Z:15S136M AS:i:146 XS:i:54 A00126:224:H7HMWDSX3:3:1106:11487:3787 113 Test_seq 168500 60 151M = 212611 44112 TCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGC FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:136A14 MC:Z:151M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1106:11487:3787 177 Test_seq 212611 16 151M = 168500 -44112 CAATCAGGCTAGACAAAGAAATAAAAGGTATCCGGATTGGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGT FFFFF::F,FFF:FFFFFF,FFFFFFFFFFFFFFFFFFF:F:FFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:10 MD:Z:4T20G2C3T13G8A5T20C0T17T49 MC:Z:151M AS:i:101 XS:i:91 XA:Z:Test_seq,+168277,151M,12; A00126:224:H7HMWDSX3:3:1106:7762:4163 81 Test_seq 170515 60 83M12I56M = 170302 -352 GTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATAGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTAT FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:21 MD:Z:8A14G23A5A10T29G2A3A3T33 MC:Z:151M AS:i:76 XS:i:29 A00126:224:H7HMWDSX3:3:1106:7762:4163 161 Test_seq 170302 60 151M = 170515 352 TTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTG FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:122C28 MC:Z:83M12I56M AS:i:146 XS:i:66 A00126:224:H7HMWDSX3:3:1106:25735:4163 81 Test_seq 423680 60 124M27S = 423431 -373 CTAAACGAATACCATCCCAGACCCTCTGACATCTAACTACATTTAAGTGCACCATCTGATCTAAGAAAGCATACAAGTTCCCAATGTCTGGTGTCTTCTACCATGTTACTCATTATAAAGGACTAGTCTCTGTCCAAGTAGTCCAAAAAGT :FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:0 MD:Z:124 MC:Z:151M AS:i:124 XS:i:0 A00126:224:H7HMWDSX3:3:1106:25735:4163 161 Test_seq 423431 60 151M = 423680 373 TCCTTCTTACTGTGGTTATGGCCATGATTTCAGCTTGAGAACCTAAGAAACTATTCACATAAAAATAATAACTTTCAAATTTATTCAAAACTCAAGTGAGGCCTTTAGTGTTGTAATATGGTTAGATAATAAAACAGATTCAAATATATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:124M27S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1106:13783:4977 97 Test_seq 167147 42 151M = 167403 389 TAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAG FFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:FFFFFFFFFFFF:FF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFF:FFFFFFFF:FFF,FF,FFFF::FF,FFFF,F:FFFFFFFF:FFF:FFFFFFF NM:i:1 MD:Z:96T54 MC:Z:133M18S AS:i:146 XS:i:126 XA:Z:Test_seq,-213737,151M,5; A00126:224:H7HMWDSX3:3:1106:13783:4977 145 Test_seq 167403 20 133M18S = 167147 -389 TGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCTTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCACTCTACTTTGAGGAGGC FFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:39T47C22A0T21 MC:Z:151M AS:i:113 XS:i:103 XA:Z:Test_seq,+213499,18S133M,6; A00126:224:H7HMWDSX3:3:1106:28302:5948 97 Test_seq 168171 29 151M = 168463 443 GGGGGCTACTGTGAATGGCATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATTTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGACGAT FF:FFFFF:F,F:FFFF:,F,FFFFFFF,FFFFFFFFFFFF:F,:FFFFFFFFF:FF,:F:FF,FFFF:F:FFFFFFF::FFF:FFF,FFFFFFFFFFF:FF,FF,FFFFFFFFFFFF::,FFF::FF,FFFF:FFF,FFFFFFF:F:FF: NM:i:4 MD:Z:18T68G12T46G3 MC:Z:151M AS:i:132 XS:i:117 XA:Z:Test_seq,-212717,151M,7; A00126:224:H7HMWDSX3:3:1106:28302:5948 145 Test_seq 168463 60 151M = 168171 -443 AGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAG FFFFFFFFFF,FFFFFF:FFF:FFFFFF:FFF,FFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFF:FFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F NM:i:1 MD:Z:32T118 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1106:25427:6042 97 Test_seq 171093 60 151M = 171329 374 CCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGACTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,:,,FFFFFFFFFFF:FFFFF:FF,FFFFFFFFFFFFFFFFFFF:FFF:FFFFF NM:i:1 MD:Z:39C111 MC:Z:138M13S AS:i:146 XS:i:58 A00126:224:H7HMWDSX3:3:1106:25427:6042 145 Test_seq 171329 60 138M13S = 171093 -374 CTTTCATCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCACCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTCGATAGAGTTCCACATGATTAATCTAGAGCATG FFFFFFFFFFFFFFFFFFFFFF:F:FF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:5G25G39G0C14T30T8C10 MC:Z:151M AS:i:103 XS:i:52 A00126:224:H7HMWDSX3:3:1106:16152:6417 81 Test_seq 171154 60 151M = 170871 -434 GTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTGGGATCAATCTTAAATGGGGTT FFFFFFFFFFF:FFF:,FFFFFFF:FFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF,FFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:: NM:i:1 MD:Z:129T21 MC:Z:151M AS:i:146 XS:i:58 A00126:224:H7HMWDSX3:3:1106:16152:6417 161 Test_seq 170871 60 151M = 171154 434 GTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGCATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTTAGCATTTCTTGCAGTCTTGGTTTAGTTTTTGCAAATTCTCTAAACTTGTGTTTATCTGTAAA F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:F,FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FF:FFF NM:i:2 MD:Z:38T49A62 MC:Z:151M AS:i:141 XS:i:23 A00126:224:H7HMWDSX3:3:1106:10221:9768 97 Test_seq 169867 60 151M = 170207 491 TGTGCGCTAGAAAAAAAAGTATACTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCAGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGTGGTG :F,FFFFFFF:FF:FFFF:F,FF,FF:,FFF:FFFFFFFFFFF,FFFFFFFFFFFFFF:FFF:F:,,F,:FFF::FFFFFF:FF,FFF,:F::FFFF:,FF:F:F:FFF:F,FFFFFF,FFF,F::,FFFFFFFFFFFF:,FF,FFFFF,F NM:i:5 MD:Z:5A17T35T38C8G43 MC:Z:151M AS:i:126 XS:i:38 A00126:224:H7HMWDSX3:3:1106:10221:9768 145 Test_seq 170207 60 151M = 169867 -491 TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC :FFFFFF,:FFFF:,:F,FFFFFFF:FF:FFFFFFFFF:FFF::FF,,FFFFF:FFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFF:F::FFF:FFFFF:F:FFFF,:FFFFFFFFFFFFFFF:FFFFF:F:FF:FFFFFFF:FFFFFFFF NM:i:1 MD:Z:55A95 MC:Z:151M AS:i:146 XS:i:88 A00126:224:H7HMWDSX3:3:1106:12861:13933 97 Test_seq 171543 60 151M = 171787 395 CCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTACTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTGTTAATTTTTCCACTATG FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,F,FFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFF:FFFFF:FFFFFF NM:i:5 MD:Z:40T9T13T68A14C2 MC:Z:151M AS:i:128 XS:i:60 A00126:224:H7HMWDSX3:3:1106:12861:13933 145 Test_seq 171787 60 151M = 171543 -395 AAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGGCTGCTGTCTC FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF: NM:i:1 MD:Z:140A10 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1106:18954:14497 81 Test_seq 168223 42 123M28S = 167949 -397 TTGTTGATGTAGAGGACTCCAAGTGATTTTTTTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCGATCTACGTCCAAAAAATCAGCCAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFF:FFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:16A14G16T74 MC:Z:151M AS:i:108 XS:i:88 XA:Z:Test_seq,+212703,38S113M,5; A00126:224:H7HMWDSX3:3:1106:18954:14497 161 Test_seq 167949 60 151M = 168223 397 AAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGA FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFF NM:i:1 MD:Z:111C39 MC:Z:123M28S AS:i:146 XS:i:80 A00126:224:H7HMWDSX3:3:1106:13404:16344 97 Test_seq 166073 60 151M = 166401 479 CTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAATTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFF NM:i:1 MD:Z:73G77 MC:Z:151M AS:i:146 XS:i:74 A00126:224:H7HMWDSX3:3:1106:13404:16344 145 Test_seq 166401 60 151M = 166073 -479 CCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACATTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACAGATTGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:4 MD:Z:51G39C12T39T6 MC:Z:151M AS:i:131 XS:i:37 A00126:224:H7HMWDSX3:3:1106:18222:16736 97 Test_seq 168314 11 151M = 168480 317 TGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGATAACTTTACTTCTTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCCTGATTGCCCTGGCTAAGACTTCCAACACGATGTTGAATAAGAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F:F:FFFFF NM:i:15 MD:Z:22C0A6C0G0G18A5G9C12T7C20A13G6A1G0T17 MC:Z:151M AS:i:76 XS:i:68 XA:Z:Test_seq,-212574,151M,17; A00126:224:H7HMWDSX3:3:1106:18222:16736 145 Test_seq 168480 60 151M = 168314 -317 TCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGG :FFFFFFFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:15T135 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1106:18747:17018 81 Test_seq 170691 60 73M4I74M = 170380 -458 CACCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGATCATTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCT FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:40G106 MC:Z:151M AS:i:132 XS:i:0 A00126:224:H7HMWDSX3:3:1106:18747:17018 161 Test_seq 170380 60 151M = 170691 458 ACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTT FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFF NM:i:1 MD:Z:44C106 MC:Z:73M4I74M AS:i:146 XS:i:53 A00126:224:H7HMWDSX3:3:1106:16251:18740 65 Test_seq 308334 60 151M = 225248 -83087 TCCATAACATACCACTGTAGTGCCCCACATAGAATCCATTTTTATAATATGCTTAGCTTAGTTGTCTTAGTTATCCAGTGCTGCTATAACAGAAATAACACAAGTGGATGGCTTTAACAAACAGAAAGTTATTCTCTACAGTCTAGGAGGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:29M122S AS:i:151 XS:i:35 A00126:224:H7HMWDSX3:3:1106:16251:18740 129 Test_seq 225248 60 29M122S = 308334 83087 GTTCTGATGTTGTTGTCATTTACAGATTATAAGTCAATCTCTTTTGAGATATAAAAGAGAGAAGTGAGGAGAAAGACATGGAGAACTCATGCTACCAAGAAACAAGATCCAGGAGAATAGCATGTCCTTTGGACCCAGGGTCCCCGTGGCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FF,FFFFFFFFFFFFF:FF NM:i:0 MD:Z:29 MC:Z:151M AS:i:29 XS:i:0 SA:Z:Test_seq,308589,-,122M29S,60,0; A00126:224:H7HMWDSX3:3:1106:16251:18740 2193 Test_seq 308589 60 122M29H = 308334 -377 AGCCACGGGGACCCTGGGTCCAAAGGACATGCTATTCTCCTGGATCTTGTTTCTTGGTAGCATGAGTTCTCCATGTCTTTCTCCTCACTTCTCTCTTTTATATCTCAAAAGAGATTGACTTA FF:FFFFFFFFFFFFF,FF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF NM:i:0 MD:Z:122 MC:Z:151M AS:i:122 XS:i:24 SA:Z:Test_seq,225248,+,29M122S,60,0; A00126:224:H7HMWDSX3:3:1106:31901:18756 65 Test_seq 115194 24 5S23M123S = 56593 -58602 TGCTCTGTCACATGGGGTTGCTATGAGTAGTCAAGCTTTTGGGACCTTCATTTACTGATATGGCATAACTCAAAATGAGAAGAAATAGCTGCAAACATTCAGTAATAATCAGAATATGGAATGTACAAAATATAAATCTAGGAAAGTTGGA FFFFFFFFF:FF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:23 MC:Z:151M AS:i:23 XS:i:0 SA:Z:Test_seq,56816,-,123M28S,60,0; A00126:224:H7HMWDSX3:3:1106:31901:18756 2129 Test_seq 56816 60 123M28H = 56593 -346 TCCAACTTTCCTAGATTTATATTTTGTACATTCCATATTCTGATTATTACTGAATGTTTGCAGCTATTTCTTCTCATTTTGAGTTATGCCATATCAGTAAATGAAGGTCCCAAAAGCTTGACT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:FFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:123 MC:Z:151M AS:i:123 XS:i:0 SA:Z:Test_seq,115194,+,5S23M123S,24,0; A00126:224:H7HMWDSX3:3:1106:31901:18756 129 Test_seq 56593 60 151M = 115194 58602 CTTCCGTATGGATATTATCCTATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCCAAAATGGCCA FFFFFF,,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFFFFF:FFFFFFFFFFF:,FFFFFFFFFFFFF:,FFFFFFF: NM:i:0 MD:Z:151 MC:Z:5S23M123S AS:i:151 XS:i:103 A00126:224:H7HMWDSX3:3:1106:24795:22357 81 Test_seq 169530 57 151M = 169327 -354 TTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCAGCCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:8 MD:Z:27C11G58T5T0T0T3A22G17 MC:Z:151M AS:i:111 XS:i:78 A00126:224:H7HMWDSX3:3:1106:24795:22357 161 Test_seq 169327 60 151M = 169530 354 GCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFF,FFFFF:FFFF:FF:FFFF:FFFFFFFFFFFFFF NM:i:1 MD:Z:140G10 MC:Z:151M AS:i:146 XS:i:111 A00126:224:H7HMWDSX3:3:1106:9127:23187 81 Test_seq 169083 60 151M = 168739 -495 TTCAATCTGTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACGTAGTAATCTGAAGTGATT FFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:8C62G2T3G7C31C26A5 MC:Z:151M AS:i:116 XS:i:65 A00126:224:H7HMWDSX3:3:1106:9127:23187 161 Test_seq 168739 60 151M = 169083 495 AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF: NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:81 A00126:224:H7HMWDSX3:3:1106:17309:23328 81 Test_seq 168660 60 151M = 168424 -387 ATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:56T94 MC:Z:151M AS:i:146 XS:i:55 A00126:224:H7HMWDSX3:3:1106:17309:23328 161 Test_seq 168424 60 151M = 168660 387 ATTGCCCTGGCTACGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCCGTTGGCTTTGCATAGATGCCCTTTATTA F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:13G57T50T28 MC:Z:151M AS:i:136 XS:i:67 A00126:224:H7HMWDSX3:3:1106:17707:24392 97 Test_seq 168738 60 151M = 168980 393 AAACCAGCCTTGCAGACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGCATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFF:FFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFF NM:i:2 MD:Z:14T54G81 MC:Z:151M AS:i:141 XS:i:81 A00126:224:H7HMWDSX3:3:1106:17707:24392 145 Test_seq 168980 60 151M = 168738 -393 TCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTCCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGA FFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:1 MD:Z:104T46 MC:Z:151M AS:i:146 XS:i:63 A00126:224:H7HMWDSX3:3:1106:27308:24862 113 Test_seq 213950 37 151M = 167162 -46789 AATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTTATACGCTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTTTCTTAAACAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCG F:F:::FFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:28C14A49T11A45 MC:Z:151M AS:i:131 XS:i:112 XA:Z:Test_seq,+166938,4S147M,7; A00126:224:H7HMWDSX3:3:1106:27308:24862 177 Test_seq 167162 52 151M = 213950 46789 TAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:81T69 MC:Z:151M AS:i:146 XS:i:121 XA:Z:Test_seq,+213722,151M,6; A00126:224:H7HMWDSX3:3:1106:2284:29434 97 Test_seq 167173 44 28S123M = 167393 371 GTGAAGATGCATCTACATGCCAAGGATCGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGAC :FFFFF:::FF:F:FFFF::F:,F:,F:FFFFFF:F,:FFFFF:FFFFFFFF:FFFFFFFFF,:FF,FFFF:FF,FFFF,FF,F:F:F:,FFF:FFFF,:FFF:F::F,F,:F,F,FFF:FFFFFFFFF,FFFF:,F::FFF,FFFFFFF, NM:i:2 MD:Z:5A64T52 MC:Z:151M AS:i:113 XS:i:93 XA:Z:Test_seq,-213739,123M28S,6; A00126:224:H7HMWDSX3:3:1106:2284:29434 145 Test_seq 167393 60 151M = 167173 -371 AGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATCTTTATCGT FF:FFFFFFFFF:,,FFFF:,FFFFFFFFFFFFF:FFFFFF,F,FFFF,FFFF:FF:FFF:FF:FF:F:FF,FFFFFFFFF:FFFFFF,:FFFFF,,FFFFFFFF,:FFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F NM:i:1 MD:Z:118T32 MC:Z:28S123M AS:i:146 XS:i:117 XA:Z:Test_seq,+213491,29M4D4M4I114M,10; A00126:224:H7HMWDSX3:3:1106:3125:29763 81 Test_seq 170310 60 151M = 169989 -472 GTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATT FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:66C84 MC:Z:151M AS:i:146 XS:i:63 A00126:224:H7HMWDSX3:3:1106:3125:29763 161 Test_seq 169989 48 151M = 170310 472 GGATGTCCTGTCCTTCTCCGAAAGTGGTGTGTTAAAGTCTCCTACTATAATTGTGGAGGTATCTATATCACTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATTTCT ,FFFF:FFF,FF,:FF:F,,FFFFF::F,FF,:F:FF:FFF,FFFFFFF,FFFF,FFFF:FFFFFFFFFFF:FFF:FFFFFF,F:F:F:FFFFFFFFFF,FFF,FF,F,F,,:F,F:FFFF,,FFFFFFFFFFFFFFFFFFF,FFFF,::F NM:i:10 MD:Z:33G5C10A7A1G5C2G48C7G20A3 MC:Z:151M AS:i:102 XS:i:73 A00126:224:H7HMWDSX3:3:1106:29026:29841 65 Test_seq 383472 0 5S21M125S = 166159 -217314 GCCTTGAGTTGCAATAGTGAAGGATTGATCAATCAGGAGATAAGAACTGCTTTGGATGAAGGGAAAGACAACACACAATACAAGGAAGGTCAGCCTAATTGGACTGGACTAAAAGCAAAGAGGTTTCCGGGATAAAATGAAAGCTTCAAAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:21 MC:Z:151M AS:i:21 XS:i:21 SA:Z:Test_seq,166429,-,128M23S,60,2; XA:Z:Test_seq,-334091,125S26M,1; A00126:224:H7HMWDSX3:3:1106:29026:29841 2129 Test_seq 166429 60 128M23H = 166159 -398 CTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCATCCAAAGCAGTTCTTATCTCCTGATTGATCAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:95C18A13 MC:Z:151M AS:i:118 XS:i:28 SA:Z:Test_seq,383472,+,5S21M125S,0,0; A00126:224:H7HMWDSX3:3:1106:29026:29841 129 Test_seq 166159 60 151M = 383472 217314 CAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGGTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGAC FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:::FFFFFF NM:i:1 MD:Z:102T48 MC:Z:5S21M125S AS:i:146 XS:i:77 A00126:224:H7HMWDSX3:3:1106:15591:30123 97 Test_seq 171134 60 151M = 171323 322 TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTG :FF:FFFFFF:,FF:F:F,,FF:F:F,F::,FFFFF,FFFF,:::::F:FFFFFFF,,FF::F:F:FFFFFFF:FFFFFF,,F,FFF,:FFFF:F,,:F,F,,,FF:,::FFF,,FF,FF,,FF:F,,,FF,FF,FFFF,F,FFFF,F,:F NM:i:1 MD:Z:118T32 MC:Z:133M18S AS:i:146 XS:i:63 A00126:224:H7HMWDSX3:3:1106:15591:30123 145 Test_seq 171323 60 133M18S = 171134 -322 GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAG ::::,FFF,:F:FFF,FFF:FF,:FF:FF:FF:FF,F:,F:F:::FF::F,FFFFF,,,,,FF:F::::F,,FF,F,FFFFFF:FFF,F:FF:F::FF,FF,FFF,F,FFFFFF,F,:FF,FFFF:F,FFFFFF:FF::FFFFFF:FF:,F NM:i:3 MD:Z:46G30G15T39 MC:Z:151M AS:i:118 XS:i:38 A00126:224:H7HMWDSX3:3:1106:30138:30483 81 Test_seq 183564 60 151M = 183328 -387 TGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCACTGACCCT FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:,FFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFF:FFFFF:FFFFFFF:FFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:26M4I121M AS:i:151 XS:i:52 A00126:224:H7HMWDSX3:3:1106:30138:30483 161 Test_seq 183328 60 26M4I121M = 183564 387 TGTTCTGCATCTATTAAAAATGATGAGATCGATCCATGTAGCTGTAGCAGTGGTGGGTTAGAGTTTTAGGTCTGTAGCCAGTAGATGTATGTTTGTATTTTCTCTCTGCCACTTACTAGTTGTGTTAATTTGAGCAAGTCATTTGACCTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFF::FFFFFF NM:i:4 MD:Z:147 MC:Z:151M AS:i:137 XS:i:0 A00126:224:H7HMWDSX3:3:1106:15890:30639 97 Test_seq 171134 60 151M = 171323 322 TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,::FFFFFFFFFFFF,:FF:F:FFFF,FFFFFF:FF:FFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FF NM:i:1 MD:Z:118T32 MC:Z:133M18S AS:i:146 XS:i:63 A00126:224:H7HMWDSX3:3:1106:15890:30639 145 Test_seq 171323 60 133M18S = 171134 -322 GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FF,:FFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:46G30G15T39 MC:Z:151M AS:i:118 XS:i:38 A00126:224:H7HMWDSX3:3:1106:15881:31344 97 Test_seq 168469 60 151M = 168551 207 GATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTA FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:26T124 MC:Z:125M26S AS:i:146 XS:i:94 A00126:224:H7HMWDSX3:3:1106:15881:31344 145 Test_seq 168551 60 125M26S = 168469 -207 GCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCGATCTCTCCCTCCTTTTATCCTTCCT :::FF:FF,FFFF,FFFF:F,::FFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:1 MD:Z:112T12 MC:Z:151M AS:i:120 XS:i:70 A00126:224:H7HMWDSX3:3:1106:9118:32095 97 Test_seq 171491 60 151M = 171785 445 ATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGTTTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCAATTTCTGTAATTTTATTGTTAATCTTTTGGATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:FFF:FFFFFFFFFFFFFFFFFFFF:FFFFFF:FFF NM:i:5 MD:Z:34A4A52T23T1A32 MC:Z:151M AS:i:126 XS:i:42 A00126:224:H7HMWDSX3:3:1106:9118:32095 145 Test_seq 171785 60 151M = 171491 -445 TTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTGGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTC FFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:90T60 MC:Z:151M AS:i:146 XS:i:25 A00126:224:H7HMWDSX3:3:1106:14362:33599 97 Test_seq 167148 41 151M = 167421 424 AATTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF::FF,FFFF:FFF:FFFFFF,F,FFFFFFF,F:FFFF,:FFF NM:i:2 MD:Z:30A64T55 MC:Z:151M AS:i:141 XS:i:121 XA:Z:Test_seq,-213736,151M,6; A00126:224:H7HMWDSX3:3:1106:14362:33599 145 Test_seq 167421 60 151M = 167148 -424 TTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTGGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATC FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFF:FF:,FFFFFFFFFFFF:FFFFF:F,FFFFFF NM:i:1 MD:Z:85A65 MC:Z:151M AS:i:146 XS:i:106 A00126:224:H7HMWDSX3:3:1106:27236:35697 81 Test_seq 407226 60 151M = 406947 -430 GTGCAGCTTGTCCTTGCTCTTCAACTTTGTGTTGAGAAGTAAATAATAAGCTTGAATGCTCTTTTGCAGATAATCCTAACAGGGGAACCAACACTATAGCTAGAGATTCAGGAGAATTTGAAAAGAGGTTTACTCATTTGGAAATGGGAGA F,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFF NM:i:0 MD:Z:151 MC:Z:61M4I86M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1106:27236:35697 161 Test_seq 406947 60 61M4I86M = 407226 430 TCCAGGGACCTCAGCTATTCCATGAGATAATTTTACAAATGGTAGCCAGTGTCCTGTTTCAGATCGATCACAAATGTCCCTGTGGCCCCACTAGTGTCTTAAACATTATATAGGAACAAGGCTTTGCAGGGCAAAGACAGCAGTCCACTAG FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:F:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:147 MC:Z:151M AS:i:137 XS:i:0 A00126:224:H7HMWDSX3:3:1106:29008:36886 81 Test_seq 168180 42 151M = 167972 -359 TGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGG FFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F:FFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:91T59 MC:Z:151M AS:i:146 XS:i:126 XA:Z:Test_seq,+212708,151M,5; A00126:224:H7HMWDSX3:3:1106:29008:36886 161 Test_seq 167972 37 151M = 168180 359 TCGGAAGTGCGTTAAATGTATAGATGGCATTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGTAGAGCTTTGTAGTTTTCTTTCTATAG FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFF:FF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:10 MD:Z:1T12T13T50G8C35C4T3A11G1G3 MC:Z:151M AS:i:108 XS:i:86 A00126:224:H7HMWDSX3:3:1107:20654:1313 113 Test_seq 434885 60 151M = 382600 -52411 AAAAAAAAAAAAATTAAAATACACTCCTTGTATGTGACAGTAAAGATGGTCAGTATAGCTTTAACGTGTTATCTGTATGTCTGCATGTCTTTGTACCTGAACACCTTTGTGTATTTAATCACATATGGTATTTGGCAGACTGTGGATTCTG FFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:125S26M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:20654:1313 177 Test_seq 382600 35 125S26M = 434885 52411 TGGAATTCTAGGTCTTCAAAGTGTTTGCTCTCAACATCCAACAGGTCAGCATCCTTTGGGGAAAAGGGAATGAAAACAAGACACACCAGAATGGCTGTAGCCTTAACATGAAGAAGCAATTATTATAATATGACTATTATTCAAATTTACT FFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:25A0 MC:Z:151M AS:i:25 XS:i:0 SA:Z:Test_seq,434625,+,26S125M,60,0; A00126:224:H7HMWDSX3:3:1107:20654:1313 2209 Test_seq 434625 60 26H125M = 434885 411 TAATAATTGCTTCTTCATGTTAAGGCTACAGCCATTCTGGTGTGTCTTGTTTTCATTCCCTTTTCCCCAAAGGATGCTGACCTGTTGGATGTTGAGAGCAAACACTTTGAAGACCTAGAATTCCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:125 MC:Z:151M AS:i:125 XS:i:0 SA:Z:Test_seq,382600,-,125S26M,35,1; A00126:224:H7HMWDSX3:3:1107:4788:1892 97 Test_seq 166087 60 151M = 166358 422 GGAGTTGTCTGTTGTGTTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTT FFFFFFFFF:F:FFFF:FFF:,FFFF:FFF,FFFFFFFFFFFFFFFFFFFF,FF,FFFF::FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFF:FFFF,FFFFFFFFFFFF,F:FFFFFFFF NM:i:1 MD:Z:16C134 MC:Z:151M AS:i:146 XS:i:74 A00126:224:H7HMWDSX3:3:1107:4788:1892 145 Test_seq 166358 60 151M = 166087 -422 ATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTG ,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:43 A00126:224:H7HMWDSX3:3:1107:7120:2801 97 Test_seq 165764 60 16S135M = 166060 447 ACTTGCTCTCCCCCTATTCAGTCTCTTGTTTCGTGCCAATTTTGCCATCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTGATTTAATTAGCTCACTCTTCATCAGCATCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:10C25T66A8G22 MC:Z:151M AS:i:115 XS:i:0 A00126:224:H7HMWDSX3:3:1107:7120:2801 145 Test_seq 166060 60 151M = 165764 -447 GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAAGGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCT FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:107T43 MC:Z:16S135M AS:i:146 XS:i:75 A00126:224:H7HMWDSX3:3:1107:29279:4225 97 Test_seq 167906 60 151M = 168198 443 TTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:41 A00126:224:H7HMWDSX3:3:1107:29279:4225 145 Test_seq 168198 39 151M = 167906 -443 GGTTATTTCCTCTTCGATGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTTTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATC :FFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:4 MD:Z:16G56T18C55G2 MC:Z:151M AS:i:133 XS:i:113 XA:Z:Test_seq,+212690,151M,8; A00126:224:H7HMWDSX3:3:1107:9968:5729 97 Test_seq 441645 60 31S120M = 441869 375 TTCCACAGGATTTTCAAAGAGCAGATGGATTGATCATTCAGTTTCATTCTTTCCCTTCATTACTCCAAAATCTATTCTCTAAATACCCTTTTCCATAATAGCAAATATTTCTTGATTTCTATTCCTTTGTGGAGATGCTTGCCATTTACAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFF:FFFFFFFFFF:F,FFFFFFF:F:FFFFFF::F,FFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:120 MC:Z:151M AS:i:120 XS:i:0 A00126:224:H7HMWDSX3:3:1107:9968:5729 145 Test_seq 441869 60 151M = 441645 -375 TGTTTTACTAAAATAAATATTCTCTCGAGTAGGTTGATAGTGACTTTGGTCTTTGAATCACTTATGTGTTTTAAAAACGATGTACATATTTTATAATTTGTTTAACAGATTTACACATTCAGTATTTTGGGTAATTAAGTAAAATACATGA FFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:31S120M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:1597:6825 81 Test_seq 213902 52 151M = 213742 -311 TTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTTATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGTGTTTTCTTA ,FFFF,FFFFFFFFFFF:F,:F:,:FFFFFF:FFFFFFFFFFF,F:F,FFFF,FF:FFFFFFF,FFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFF:F,FFFFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFF:FFFF:FFFFFFFF NM:i:1 MD:Z:76C74 MC:Z:19S132M AS:i:146 XS:i:121 XA:Z:Test_seq,+400403,151M,6; A00126:224:H7HMWDSX3:3:1107:1597:6825 161 Test_seq 213742 19 19S132M = 213902 311 GTAATGGTGCTCTGGGATTAAAACTCAACCACAAAAAGACAAACAACCCAATGAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAACTAACAGATATATGAGAAAATGCTCTCGATCATTAACCATTAT FFFFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFF:FFFFFFFF:FF,:FFFFFFFFFFFFFFFFFFFFF:F,FFFF:FFFFF:FFFF:FFFFFFFFFFFF,:FF:FF:FFFFF:F:FF:FF:F::FFFF, NM:i:7 MD:Z:17G6G8C54G10C24G6G0 MC:Z:151M AS:i:101 XS:i:91 XA:Z:Test_seq,-167161,132M19S,9; A00126:224:H7HMWDSX3:3:1107:3414:7122 65 Test_seq 140816 0 52M = 384324 243509 TCCTCTTCACTTTCAGCAAGCAAGGTTGTGTCATCTGCATAATGCAGGTTGT FFFFFFFF:FFFFFFFFFF::FFF:FF::FFFFFFFFFFFFFFFFFFFF:FF NM:i:1 MD:Z:32T19 MC:Z:52M AS:i:47 XS:i:47 XA:Z:Test_seq,+333187,52M,1;Test_seq,-384324,52M,1;Test_seq,-366380,50M2S,1;Test_seq,-17092,42M10S,0; A00126:224:H7HMWDSX3:3:1107:3414:7122 129 Test_seq 384324 0 52M = 140816 -243509 ACAACCTGTGTTATGCAGGTGACACAATCTTGCTTGCTGAAAGTGAAGAGGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF NM:i:3 MD:Z:8C9A8C24 MC:Z:52M AS:i:37 XS:i:37 XA:Z:Test_seq,-333187,52M,3;Test_seq,-315912,52M,4; A00126:224:H7HMWDSX3:3:1107:30897:7185 81 Test_seq 171715 60 151M = 171468 -398 TTTTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGAT FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:43G107 MC:Z:151M AS:i:146 XS:i:29 A00126:224:H7HMWDSX3:3:1107:30897:7185 161 Test_seq 171468 60 151M = 171715 398 GTAGGGTTTCTTCATTTTTTTAAATTCTTTTATCTGATTTTTTTTCAGCTACGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGATGTCTAATTCTGTAAT ,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFF NM:i:5 MD:Z:0T20T29T63T18T16 MC:Z:151M AS:i:130 XS:i:41 A00126:224:H7HMWDSX3:3:1107:20374:7247 97 Test_seq 167160 52 151M = 167385 376 TCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFF:,FFFFFF:FFFF,FF,FFF:FFF:F:F:FFFF,FFFFFFF:FFFFFFF: NM:i:1 MD:Z:83T67 MC:Z:151M AS:i:146 XS:i:121 XA:Z:Test_seq,-213724,151M,6; A00126:224:H7HMWDSX3:3:1107:20374:7247 145 Test_seq 167385 60 151M = 167160 -376 TTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:57T44G2C22A0T2T18 MC:Z:151M AS:i:121 XS:i:72 A00126:224:H7HMWDSX3:3:1107:8594:7232 113 Test_seq 39851 6 68M = 168798 128948 CTATATCCCTCATGCCCTTAGATGCAAAAATCCTCAATAAAATTCTAGCCAATAGAATTCAACAGCAT FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF NM:i:5 MD:Z:14A0A1A19C26A3 MC:Z:68M AS:i:44 XS:i:39 XA:Z:Test_seq,+153099,68M,6;Test_seq,+168802,4S64M,5; A00126:224:H7HMWDSX3:3:1107:8594:7232 177 Test_seq 168798 14 68M = 39851 -128948 GTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCAATGTTCATGAGGGATATAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:48T19 MC:Z:68M AS:i:63 XS:i:57 XA:Z:Test_seq,+39851,68M,3; A00126:224:H7HMWDSX3:3:1107:20500:7529 97 Test_seq 167160 52 151M = 167385 376 TCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATT FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FF:FF:FFFFFFF,FF:,FFFFF,FFFFF,F,FFF:F:FF,:,:FFFFFFFF:,:FFF,,,:F:FF,:,F NM:i:1 MD:Z:83T67 MC:Z:151M AS:i:146 XS:i:121 XA:Z:Test_seq,-213724,151M,6; A00126:224:H7HMWDSX3:3:1107:20500:7529 145 Test_seq 167385 60 151M = 167160 -376 TTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTTTCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATC FFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF NM:i:7 MD:Z:54A2T44G2C22A0T2T18 MC:Z:151M AS:i:116 XS:i:72 A00126:224:H7HMWDSX3:3:1107:4743:11271 97 Test_seq 171530 60 151M = 171742 363 ATTCCCTGGTCCTACAGAAGTCCCAGTCTACATTCTAATTGCTTGAGTCTGCTCCTCTGACTTTCTCTTGCATTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTATTAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFF,F::F:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:F:F,FFFF NM:i:6 MD:Z:13C29C9T12A4G5T73 MC:Z:151M AS:i:121 XS:i:59 A00126:224:H7HMWDSX3:3:1107:4743:11271 145 Test_seq 171742 60 151M = 171530 -363 CTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCT F,:F:F:FFF:F:FFFFF:FFFFFFFFFFFF:FFFFFFF:FFFFF,:FFFFFFFFFFFFFFFFF:F,FFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:16G134 MC:Z:151M AS:i:146 XS:i:25 A00126:224:H7HMWDSX3:3:1107:15727:13573 97 Test_seq 170584 60 26S125M = 170790 357 GCTTCAGGTGACAATCAAAATAGATTAGTCGTTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATCACTCTCC FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFF,FF,F:FFFFF,FFFFFF:FFFF::FFFFFFF,F::FF,FF,FF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFF:FFFFF:FFF:FFFFFFFFF NM:i:2 MD:Z:101G6A16 MC:Z:151M AS:i:115 XS:i:37 A00126:224:H7HMWDSX3:3:1107:15727:13573 145 Test_seq 170790 60 151M = 170584 -357 TGTTATATGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTTACTTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATC FFF:,F,FFFFFFFFFF:FFF:FFFFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFF NM:i:1 MD:Z:6G144 MC:Z:26S125M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1107:5719:14184 97 Test_seq 165873 60 29M2D122M = 166128 406 ATTGGCTCACTCTTCATCAGCATCTCTCTCCCCCCCGCTGACCAGTCCTTTTCATGCCTGATGAGTTGTCTTCGGGGATGGTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCGCATTCATATCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FF:FFFFFFFF:FF:FFFF:FFFFFF,FFFFFF,F NM:i:4 MD:Z:29^CC27T82A11 MC:Z:151M AS:i:133 XS:i:0 A00126:224:H7HMWDSX3:3:1107:5719:14184 145 Test_seq 166128 60 151M = 165873 -406 GTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:29M2D122M AS:i:151 XS:i:87 A00126:224:H7HMWDSX3:3:1107:11984:14857 81 Test_seq 168480 60 151M = 168347 -284 TCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATCGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:148G2 MC:Z:25S126M AS:i:148 XS:i:84 A00126:224:H7HMWDSX3:3:1107:11984:14857 161 Test_seq 168347 60 25S126M = 168480 284 GACCTTTTTCTAGGCAATAGATCGATCATGTCATCTGCAAATAGTGATAACTTTACTTCTTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCCAGTACGATGTTGAATAAGAGCGGTGATA FFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:F NM:i:5 MD:Z:18A5G9C12T49A28 MC:Z:151M AS:i:101 XS:i:68 A00126:224:H7HMWDSX3:3:1107:5376:14904 97 Test_seq 168347 60 16S135M = 168525 329 ATGACTAGTGCTTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF NM:i:1 MD:Z:18A116 MC:Z:151M AS:i:130 XS:i:73 A00126:224:H7HMWDSX3:3:1107:5376:14904 145 Test_seq 168525 60 151M = 168347 -329 TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTGTGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFF NM:i:1 MD:Z:49A101 MC:Z:16S135M AS:i:146 XS:i:82 A00126:224:H7HMWDSX3:3:1107:5737:15029 97 Test_seq 170350 60 151M = 170514 303 GATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,F:FF:F:FF NM:i:1 MD:Z:140T10 MC:Z:84M12I55M AS:i:146 XS:i:48 A00126:224:H7HMWDSX3:3:1107:5737:15029 145 Test_seq 170514 60 84M12I55M = 170350 -303 TGTGCTGAGACGTTTTTCTTAGTAGCTTGTGAGGTCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCATTACGTTTTTCTTGGCTTTTCTCTTGAGTTATGAAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTG FFFFF:FFFFFF,FFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:17 MD:Z:25A7A40G21G41A0 MC:Z:151M AS:i:100 XS:i:27 A00126:224:H7HMWDSX3:3:1107:21911:15264 81 Test_seq 98908 60 151M = 98654 -405 CAAGGAGGGAAGGTCCAGCAGAGCAGGCTTCCAGAGGTGGGCCAGGTGCTTGTCACGGTCCTAAAATAGGGTTGGACGAAACAGGGTATTTGCTTCAGATGGGCCAGTAGTATGGCACCCCTTCAAGCTGTCACTTTAAAGACATTACTCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:21911:15264 161 Test_seq 98654 60 151M = 98908 405 GATCCATCTGTCTTGTTTATCAATGCTTATATTGCTAGCCCTCCTGCTGGAGTTTTAATTCTAGCAGATCAAGAAAACTTTTCGCTAAGGGATCTTTTTAAGTCCATCTCAGTCAGGGAGAAGCCTTCTCTTTCGCCTTTCCACCTTCACC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FF,FFFFFFFFFFFFFFFFFFFFFFFFF,FFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:19768:19758 113 Test_seq 212730 0 151M = 168466 -44265 CTACTGAAACTAATAGAAGAGTTTGGCAGAGACTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACTGAAGAGGAAATAACCAAATCAATACCGTTCACAGTAGCCCCCAAGAAGATAAGAT FFFF,FFFFFFFFFFF,FFFFFFFFFFFFFF,F:FF:,FFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFF:FFFFFF:FFFFFFF:FFFF,FFF NM:i:7 MD:Z:31T12G49T0C0A25A25A2 MC:Z:151M AS:i:118 XS:i:118 XA:Z:Test_seq,+168158,151M,7; A00126:224:H7HMWDSX3:3:1107:19768:19758 177 Test_seq 168466 60 151M = 212730 44265 GGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTT FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:86 A00126:224:H7HMWDSX3:3:1107:22318:21010 97 Test_seq 109072 60 151M = 109250 325 ACTCAGAAAAAGAGCGTGAGAATGGTTGCACAACTCATCGGTGTCACTGAATCGTACATGTAGAAACTGTTGAATTGGCGTATATTCTGCCGTTTATATTCTTAAAAACAACAACAAATAAACTGTTGTTTAAAAAAAAATACCGTCAAGA FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFF,,FFFFF:FFF,FFF,,FFFFFFFFFFFFF:FF,F::FFFFF:F NM:i:0 MD:Z:151 MC:Z:33M4I114M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:22318:21010 145 Test_seq 109250 60 33M4I114M = 109072 -325 AGAAAATATTTTTAATACGTATATTTGACAAAGGATCGATCTGTATCCAGACTACATTAAGAACTCAATAAAAAATTTTTAAACTTTCAGTTTTAAAAATGGGCATGAGTAGACATTTCACAGAAGAAGACATAAAACGTCATATTAAAAG FFFFF:F,FFFFFFFFFFFFFF::FFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:4 MD:Z:147 MC:Z:151M AS:i:137 XS:i:0 A00126:224:H7HMWDSX3:3:1107:23764:21230 97 Test_seq 189673 60 29S122M = 189974 452 AAGTCCTTACCTACTCTCTCCAGCTCTGAGATCTCATCAGCGAGTAGCTTGATTCCCCGTTTAGGGAACAGCTACCTACTAGACATTTCTAGACTCTTCCTTGCTGGGAAATATCACTAATCTGGGAGACTTACCACAATGCAGTATTATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:F:FFF,F,FFFFFFFF:FFF:F,FFFFFFFFFFFFFFF:FFFFFFF:,FFFFFF,F NM:i:0 MD:Z:122 MC:Z:151M AS:i:122 XS:i:0 A00126:224:H7HMWDSX3:3:1107:23764:21230 145 Test_seq 189974 60 151M = 189673 -452 TGCAGCAAGATATTAATATTTTAAGAGAAAATGAAATTAGAAACAAAACCTCATTCATTTGAGTGCAAAGGCTAACACAAATGGAGAATAAAAGAAATATAAAGCATTGCCCCTCCTCTCAAGAAGACTACCGTAACTTCGCAGGTACCGT ,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFF,FF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:29S122M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:24921:24361 97 Test_seq 168347 60 17S134M = 168525 329 TTCCACAGACGGATTGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGGTGAATAAGAGTGGTGATAAAGGGCAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:18A88T10C15 MC:Z:151M AS:i:119 XS:i:66 A00126:224:H7HMWDSX3:3:1107:24921:24361 145 Test_seq 168525 60 151M = 168347 -329 TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:17S134M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:23222:25551 81 Test_seq 171427 60 151M = 171161 -417 CACTCGCAAGTTATCCTTCTTGATAGAGCCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTTCAGATGTCCCAGTCTACATTCTAATTGCTCGAGT FFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:28T69A17C4A29 MC:Z:151M AS:i:131 XS:i:42 A00126:224:H7HMWDSX3:3:1107:23222:25551 161 Test_seq 171161 60 151M = 171427 417 CTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FF:FFFFFF:FFFFFFFFF:FF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFF,FFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:35 A00126:224:H7HMWDSX3:3:1107:5014:25895 97 Test_seq 168347 60 23S128M = 168591 395 TTGGGCCCTGGCACCTGGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGAATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAA FFFFFFFFFFF,F:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:18A28T1G78 MC:Z:151M AS:i:113 XS:i:65 A00126:224:H7HMWDSX3:3:1107:5014:25895 145 Test_seq 168591 60 151M = 168347 -395 TTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAAC :FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:125T25 MC:Z:23S128M AS:i:146 XS:i:39 A00126:224:H7HMWDSX3:3:1107:24108:26459 81 Test_seq 167614 6 124M27S = 167465 -273 TGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGCTCATACGTGGATGGATCGATCACTTATCCTATACTGGACCAGC :FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:61C50T1A9 MC:Z:151M AS:i:109 XS:i:104 XA:Z:Test_seq,+40970,27S124M,4;Test_seq,+213301,27S124M,4; A00126:224:H7HMWDSX3:3:1107:24108:26459 161 Test_seq 167465 60 151M = 167614 273 TAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:0 MD:Z:151 MC:Z:124M27S AS:i:151 XS:i:106 A00126:224:H7HMWDSX3:3:1107:14281:27383 97 Test_seq 171469 60 151M = 171734 416 TAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCTTATTGCATTGTCTAATTCTGTAATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFF NM:i:5 MD:Z:56A57T9T0C6G18 MC:Z:151M AS:i:126 XS:i:38 A00126:224:H7HMWDSX3:3:1107:14281:27383 145 Test_seq 171734 60 151M = 171469 -416 GTGTGTTCCTTGGCTTTTTCTGCAGTTACCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTG FFFFF:FFFF,FFFFFF,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:28T122 MC:Z:151M AS:i:146 XS:i:25 A00126:224:H7HMWDSX3:3:1107:24252:27868 81 Test_seq 167455 6 113M4I34M = 167149 -453 TCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:9 MD:Z:32G2C22A0T2T84 MC:Z:151M AS:i:112 XS:i:107 XA:Z:Test_seq,-400918,113M4I34M,10; A00126:224:H7HMWDSX3:3:1107:24252:27868 161 Test_seq 167149 42 151M = 167455 453 ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFF NM:i:1 MD:Z:94T56 MC:Z:113M4I34M AS:i:146 XS:i:126 XA:Z:Test_seq,-213735,151M,5; A00126:224:H7HMWDSX3:3:1107:3025:29340 97 Test_seq 260193 60 151M = 260357 315 AGTGATTTGCCCAAGACCACTCAGTTATAATTACATAATTGTAGAGGAATTCCAAATTTTTTATTTAAAAGAAGAAGCAGGAAACATTCTAAATCTGAAAGTATTGTAATTTTTCAAGTAATTTTTTAAAATGCCATTTGCAAATATCAAG FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFF:FFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:3025:29340 145 Test_seq 260357 60 151M = 260193 -315 GGAAATGAGACTTGTTTTAGGTGCCATCAAGCTGATTCTGACTCCTAGTGAGGCTATGTAAAACAGACCAAAACACTGTTCCTGTGCCATCCTCACAATTGTTATGCTTGAGCCCATTGTTGCAGCGACTGTGTCAGTCCATCTTGTTGAG ,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F NM:i:2 MD:Z:67A41A41 MC:Z:151M AS:i:141 XS:i:31 A00126:224:H7HMWDSX3:3:1107:24695:29888 81 Test_seq 169317 60 151M = 169081 -387 AGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGA FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:150G0 MC:Z:151M AS:i:150 XS:i:112 A00126:224:H7HMWDSX3:3:1107:24695:29888 161 Test_seq 169081 60 151M = 169317 387 GTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGA FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFF:FFFFFFF:FF:FFFFFFFFF:F:FFF,F,FF:FFFF:FFFF:F:,FFFFF:FFFFFFFF:F,FFFF:FF:,:FFFFF:: NM:i:7 MD:Z:42A37G1A37C11A1G2G13 MC:Z:151M AS:i:116 XS:i:75 A00126:224:H7HMWDSX3:3:1107:1759:30718 97 Test_seq 169163 60 151M = 169313 301 GGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATTCTTTTAATTTCATTTGGCTCTGTTGTGATGTGGTCCTTCTCATTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTATTTC FFFFFFFFFF:F:FF:FF,FFFFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFF:FFF:FFFF:FFFF,FFFFFFFFFFFF:,FFFF,:,F:FFFF:F:FFFFFFF::F:FF,FFFFFFF,FF,,FFFFFFFFFFFF,,FFF:,F:FFF:FF NM:i:8 MD:Z:0A37C11A1G2G49T6G33T4 MC:Z:151M AS:i:115 XS:i:73 A00126:224:H7HMWDSX3:3:1107:1759:30718 145 Test_seq 169313 60 151M = 169163 -301 CTTTAGTCAGTCTAGCCAATGTTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGA F,FFF:FFF,FFFF:F,F:FF,FFFF:,F,F:FFFFFF:FFFFF:,FFFFFFFFF:FFFFF:FF,FF,:FFFFFF:F:FFFFF,:FF:FFFFFFFFFFFFFF:F::FFFFFFFFF:FFFF:F:F:FFFFFFFFFFFFFFF:FF,FF:FFFF NM:i:1 MD:Z:21G129 MC:Z:151M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1107:10936:32894 97 Test_seq 168278 44 151M = 168514 387 CTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGACTATGTCATCTGCAAATACGGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGT FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF,F:F:FFFFFFF:FFF:FFFF:FFFF NM:i:8 MD:Z:4T63G0T0C16A0T27T33C0 MC:Z:151M AS:i:115 XS:i:90 A00126:224:H7HMWDSX3:3:1107:10936:32894 145 Test_seq 168514 60 151M = 168278 -387 TTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTA FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:89 A00126:224:H7HMWDSX3:3:1107:5629:33536 97 Test_seq 168347 60 18S133M = 168502 306 CCATCAAACACAGATCGATCATGTCATCTGCAAGTAGTGATAGCTTTACTTCCTCCTTGCCAATCGGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCAGTGATAAAGGGCA FFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:15A2A28T71G13 MC:Z:151M AS:i:113 XS:i:70 A00126:224:H7HMWDSX3:3:1107:5629:33536 145 Test_seq 168502 60 151M = 168347 -306 AAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATCGGTGTTGAACTTTGTCAAATGCCT ,FFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:126G24 MC:Z:18S133M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1107:4390:33865 97 Test_seq 359511 60 151M = 359787 427 TGGCAGAGCACAGCCAAATGCAAAAGCGGCTGGATTTCCAAAATGGTAGCTTAGAGGAAGGTCTTGAAGCAAATTCCCTTGAGAGCAATCCCCTAAACGTGATGGAGAGCCTAAGCCAGAAGAAATACTCTTCCAGTCTGAGATTTAAAGC FFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFF:F:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:4390:33865 145 Test_seq 359787 60 151M = 359511 -427 AGTTCTCTGGTGATGGCACTGACAGAAATCTTTCCACGAAGCCTCCCACTCCTTCAGTCAGCACCTCACCCTCCCTCGGTGGTTATCTGCTTGGCAGAGTGGAATTGGATCATTATACTGGTCGGGACAGTGAAAGGTCCTTGAGGGTCTC FFFFFFF:FFFFF::FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1107:32389:34538 97 Test_seq 168968 60 151M = 169301 484 TTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTTCAGTGAAGCCGTCCGGGCCAGGACTGTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTG :FFFFFFFFFFFFFFF::F:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFF:FFF:FFFFFFF:FFFFFFFF:F:F:F::FF:FFFF:FF,F,FF NM:i:3 MD:Z:59G13T11T65 MC:Z:151M AS:i:136 XS:i:71 A00126:224:H7HMWDSX3:3:1107:32389:34538 145 Test_seq 169301 60 151M = 168968 -484 TTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCT F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:8T142 MC:Z:151M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1107:29170:34663 113 Test_seq 167150 42 151M = 213901 46752 TTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAAT FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:93T57 MC:Z:151M AS:i:146 XS:i:126 XA:Z:Test_seq,+213734,151M,5; A00126:224:H7HMWDSX3:3:1107:29170:34663 177 Test_seq 213901 8 151M = 167150 -46752 ATTCCATCTCACACCAGCAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTCATACACTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTATCTT FFF,FFF:FFFFFFF::FFFFFFFFFFFFF,:FFFFF:FFFF:FFF:FF::,FFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:12T3A60C9T54T3T4 MC:Z:151M AS:i:122 XS:i:116 XA:Z:Test_seq,+166983,151M,7;Test_seq,+400413,9S142M,8; A00126:224:H7HMWDSX3:3:1107:14027:34741 81 Test_seq 170289 60 151M = 170044 -396 TTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:84 A00126:224:H7HMWDSX3:3:1107:14027:34741 161 Test_seq 170044 60 151M = 170289 396 GAGGTGTCTATCTTGCTTTTCAATTCTGTTAAAATTTGATTTGTGTATCTTGCAGCCCTGTCATTGGGTGCGTAAATATTTAATATGGTTATATCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGG FFFFFFFFFF:FFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFF:F:,FFFFFFFF,F,FFF,F:FFF:F,FFF:FFFFF:,,FFFF NM:i:4 MD:Z:3A9C28A20C87 MC:Z:151M AS:i:132 XS:i:82 A00126:224:H7HMWDSX3:3:1107:5041:36213 97 Test_seq 167566 28 12S139M = 167823 408 GAGCTAGGCTTGTTGATCCATTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:8C130 MC:Z:151M AS:i:134 XS:i:121 XA:Z:Test_seq,-213334,126M25S,1; A00126:224:H7HMWDSX3:3:1107:5041:36213 145 Test_seq 167823 60 151M = 167566 -408 ATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGTGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATT FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:1 MD:Z:77G73 MC:Z:12S139M AS:i:146 XS:i:90 A00126:224:H7HMWDSX3:3:1108:25816:1767 97 Test_seq 170144 60 151M = 170383 390 GATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTACTGTTTGCTTGATATATTTTTTTCCATCCTTTGAG FFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,FFF:FFFFFFF NM:i:5 MD:Z:25A25C0A63T1A32 MC:Z:151M AS:i:126 XS:i:54 A00126:224:H7HMWDSX3:3:1108:25816:1767 145 Test_seq 170383 60 151M = 170144 -390 CTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCGTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:107T43 MC:Z:151M AS:i:146 XS:i:45 A00126:224:H7HMWDSX3:3:1108:6497:1939 97 Test_seq 168461 60 151M = 168658 348 AGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:,,F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:,FF:FFFFFFFFFFFFFFFFF::FFFFFFF,FFFFFFFF,FFF NM:i:1 MD:Z:34T116 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1108:6497:1939 145 Test_seq 168658 60 151M = 168461 -348 GCATCAATTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGA FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:5T23A28T92 MC:Z:151M AS:i:136 XS:i:60 A00126:224:H7HMWDSX3:3:1108:9634:5588 81 Test_seq 212733 60 151M = 40166 -172718 CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACT F,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:104A46 MC:Z:79M3I69M AS:i:146 XS:i:116 A00126:224:H7HMWDSX3:3:1108:9634:5588 161 Test_seq 40166 1 79M3I69M = 212733 172718 CAACAGCCAACATCACCCTAAATGGGGAGAGACTGAAAGCATTCCCCTTGAGAACGGGAACCAGACAAGGATGCCTTTTATCACCACTCGTATTCAACACTGTGCTAGAGGTCCTAGCCAGAGCAATTAGGCTAGGCAAAGAAATAAAGGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFF NM:i:16 MD:Z:15T9A5C11T21G9C5T0G3T9T6G28A0T14 MC:Z:151M AS:i:74 XS:i:71 XA:Z:Test_seq,+212488,151M,16;Test_seq,-168400,151M,17;Test_seq,-50359,64M13I74M,24; A00126:224:H7HMWDSX3:3:1108:16071:6872 65 Test_seq 167548 42 151M = 36103 -131446 GTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACTG F:F,FFFFFFF::FFFFFFFF:F:,:FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF,FFF:F::FFFFF:FFF:FFFFF::,FFFF,F:F:::FFF,FF,::F:F:F,:FFFFFF:F:FF::F,FF:FFF:::,,,FFF::FFF:::F: NM:i:1 MD:Z:127C23 MC:Z:26M125S AS:i:146 XS:i:126 XA:Z:Test_seq,-213340,120M2I24M5S,4; A00126:224:H7HMWDSX3:3:1108:16071:6872 129 Test_seq 36103 10 26M125S = 167548 131446 TGCAAAGGCAATTCTGATAGGGATCTAAGGGAATAGAGAAACAAATCACTAATTTCATATGGAAAGGAAAGAACCCCCGGATAAGCAAAACATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTC FFFF:FFFF:::FFFFFFFFFF::F,,FFFFF,::F:FF::FF,FFFFFF,FFFFFF,FFFF,,FFFF,FFF::FFFFFF:F,,FFF::FF:F::FFFF,F:FFF:F,:FFF,F:FFFFFFFFF:FF,F,,F:FF,:,FFFFF,FFF,F:, NM:i:1 MD:Z:20T5 MC:Z:151M AS:i:21 XS:i:0 SA:Z:Test_seq,167824,-,126M25S,60,4; A00126:224:H7HMWDSX3:3:1108:16071:6872 2193 Test_seq 167824 60 126M25H = 167548 -402 GAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGTTTTGCTTATCCGGGGGTTCTTTCCTTTCCATATGAAATTAGTGATTTGTTTCTCTATTCCCTTA ,:F,FFF,FFFFF,:,FF:F,,F,FF:FFFFFFFFF:F,FFF:,F:FFF:F,FFFF::F:FF::FFF,,F:FFFFFF::FFF,FFFF,,FFFF,FFFFFF,FFFFFF,FF::FF:F::,FFFFF,, NM:i:4 MD:Z:0T60C24C32C6 MC:Z:151M AS:i:110 XS:i:75 SA:Z:Test_seq,36103,+,26M125S,10,1; A00126:224:H7HMWDSX3:3:1108:7292:7576 81 Test_seq 167474 60 151M = 167298 -327 GTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:19S132M AS:i:151 XS:i:104 A00126:224:H7HMWDSX3:3:1108:7292:7576 161 Test_seq 167298 21 19S132M = 167474 327 ATGAGGGTAATGATGATTAAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFF NM:i:2 MD:Z:25A44A61 MC:Z:151M AS:i:122 XS:i:112 XA:Z:Test_seq,-213605,132M19S,4;Test_seq,+400719,19S132M,6; A00126:224:H7HMWDSX3:3:1108:24126:7701 97 Test_seq 55744 60 151M = 65144 9428 TCTCAACAGAGAAGCTGCCAAAACACTGAAAATCTTGGCCTGGCCAATTTGACACACAATCTTAACCATCACAAATAGTTCGTCTTCTTGAGCAGTCCTTTGAAATCTTCTGTTCAGCTCTTTTACTTAATCATTTCTTCCTTTCAATTTA FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:0 MD:Z:151 MC:Z:123S28M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:24126:7701 145 Test_seq 65144 54 123S28M = 55744 -9428 TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTATAAGAACCAGAGGTCAGATGATGACGAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:28 MC:Z:151M AS:i:28 XS:i:0 SA:Z:Test_seq,56015,-,123M28S,60,0; A00126:224:H7HMWDSX3:3:1108:24126:7701 2193 Test_seq 56015 60 123M28H = 55744 -394 TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:123 MC:Z:151M AS:i:123 XS:i:23 SA:Z:Test_seq,65144,-,123S28M,54,0; A00126:224:H7HMWDSX3:3:1108:21061:8187 81 Test_seq 168626 60 151M = 168347 -430 ATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:90T60 MC:Z:17S134M AS:i:146 XS:i:72 A00126:224:H7HMWDSX3:3:1108:21061:8187 161 Test_seq 168347 60 17S134M = 168626 430 AAATGTTTGAACTTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCAT FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::FFFFFFFFF NM:i:2 MD:Z:18A21G93 MC:Z:151M AS:i:124 XS:i:67 A00126:224:H7HMWDSX3:3:1108:18909:8469 97 Test_seq 104742 60 31M120S = 114282 9691 CACATATCAGGTGTCAACAGGGAAGTGATCAATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTT FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:31 MC:Z:151M AS:i:31 XS:i:0 SA:Z:Test_seq,114039,+,30S121M,60,0; A00126:224:H7HMWDSX3:3:1108:18909:8469 2145 Test_seq 114039 60 30H121M = 114282 394 AATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:121 MC:Z:151M AS:i:121 XS:i:73 SA:Z:Test_seq,104742,+,31M120S,60,0; A00126:224:H7HMWDSX3:3:1108:18909:8469 145 Test_seq 114282 60 151M = 104742 -9691 CAGCACAACTGGGCCAAAGCAAAACAAAGAAATTTCCTGGGCACATCTAAACACTGCAAGGGACAGAGTAGCTGGGGTTGGCGATTGGGGACCATAGTTTTGGGGAACATCTAGGTCAACTGCAATAATGAAGTTTATTAATAAAATGTTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:81T69 MC:Z:31M120S AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1108:14498:8656 97 Test_seq 171559 60 151M = 171789 381 ACATTCTAATTGCTCAAGTCTGCTCCTCTGACTTTCTATTGCATTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGATGTCTCTCTACGGATTCTTGCAACTTATTAATTTTTCCACTATGTTCTTGAATAATCTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FF:FFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:F:FFFFFF:FFFFFF,FFFFFFFFFFFFF,FFFF:FFF NM:i:8 MD:Z:15G8T17G5T41C10T30C13C4 MC:Z:151M AS:i:111 XS:i:67 A00126:224:H7HMWDSX3:3:1108:14498:8656 145 Test_seq 171789 60 151M = 171559 -381 GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTG FFFFFFF:FFFFFF,FF:FFFFFF:FF::FFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF NM:i:1 MD:Z:149C1 MC:Z:151M AS:i:149 XS:i:0 A00126:224:H7HMWDSX3:3:1108:10031:9471 97 Test_seq 167173 41 30S121M = 167342 320 ACTTGTATATCCAGTGTCTTGCTAGTGATCGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGCTTGTCTGTTTGTGGTTGAGTTTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFF:FFFFFFFFFFFFFFF NM:i:4 MD:Z:5A64T25T6T17 MC:Z:151M AS:i:101 XS:i:81 XA:Z:Test_seq,-213741,121M30S,8; A00126:224:H7HMWDSX3:3:1108:10031:9471 145 Test_seq 167342 21 151M = 167173 -320 AGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTG FFF:FFFFFFFF,FFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFF:::FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:26A124 MC:Z:30S121M AS:i:146 XS:i:136 XA:Z:Test_seq,+213542,151M,3; A00126:224:H7HMWDSX3:3:1108:15148:12007 81 Test_seq 169549 60 151M = 169307 -393 GCATTTATCAATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTAT FFFFFF:FFFF:FFFFF:FFFFFFFF:FFFFFFFFFF::FF:F:F:F::F:F:FFFFFFF::FFFFFFFFF:FFF:FFFF:,FFFFFFFFFFFF::,FFF:FF,,FFFFFF,FFF:FFFFFFFFFF:FFFFFFFFFF:FFF,FF,FFF:FF NM:i:3 MD:Z:9G69T34G36 MC:Z:151M AS:i:136 XS:i:28 A00126:224:H7HMWDSX3:3:1108:15148:12007 161 Test_seq 169307 60 151M = 169549 393 GTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCGGGT FFFFFFFFFFFFFF,FF:FFFFF::FFFFFFFF:FF:F,,FFFFFF:FFFFFFFFFFFFFFFF::FFFFF:FFFFFFFF:FFFFFFF,FFFFFF:FFF:FFFFF:FFFFFF:FFFF::F:FF:F,,FFFF::FF:FFFFFFFFF:FFFFFF NM:i:1 MD:Z:147T3 MC:Z:151M AS:i:147 XS:i:103 A00126:224:H7HMWDSX3:3:1108:8576:12962 97 Test_seq 169329 60 151M = 169665 483 CAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF: NM:i:0 MD:Z:151 MC:Z:4S147M AS:i:151 XS:i:111 A00126:224:H7HMWDSX3:3:1108:8576:12962 145 Test_seq 169665 60 4S147M = 169329 -483 TGACGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTATTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGTTTCATTGCCTTGTGGTCTGAGAAGATGCTTTGTAATATTTCGATGTTTTGGAC FFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:36G45C10C52T0 MC:Z:151M AS:i:131 XS:i:76 A00126:224:H7HMWDSX3:3:1108:19253:12884 97 Test_seq 146453 60 151M = 146683 381 CCTATTGGCTGACTGTAGATAACACACTGTTAGGTTCTGGGAGGCTGTTTACTACTTGAGAGCAGAGTGTTTTTGCAACACTCTATTGACCCAAAACACAAGGTTCCCAACGTTTAGGTAAGGAGCCCTGGTGGTGCAATGGTTAAGCACT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:19253:12884 145 Test_seq 146683 60 151M = 146453 -381 GAGATTACAACCTGGGAAATCCTGTGGGGCACGTTCTGCTCTATTATATGGGGCTGCTGTGACTCGGAATCTACCTGACATCACACAATAGTGCTGAGGTTAAATGCTTTATGTTTGGGGTGTACAGTTGTGGTAATTCTTCCTTCTGTGG FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:25455:13761 97 Test_seq 169282 60 151M = 169627 496 ATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:::FFFFFFFFFFFF:FFF:FFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:98 A00126:224:H7HMWDSX3:3:1108:25455:13761 145 Test_seq 169627 60 151M = 169282 -496 TCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTATTATTGATTTCTAGCTTCATTGCCTTGTGGTCTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFF:F::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:1T34G79G3C30 MC:Z:151M AS:i:134 XS:i:0 A00126:224:H7HMWDSX3:3:1108:23674:14653 97 Test_seq 166058 60 151M = 166319 412 CTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTCGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTT FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFF,F:FFFF:FFFFFFFFF NM:i:1 MD:Z:87A63 MC:Z:151M AS:i:146 XS:i:75 A00126:224:H7HMWDSX3:3:1108:23674:14653 145 Test_seq 166319 60 151M = 166058 -412 CACAATTCAAAGTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTAGAAGTCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTTCGTTGACCTTTGAAGCTTTCATTTTATCTCGGAAACCTCTTTACTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:9 MD:Z:23A18A8G14T10T24C2C25C13G5 MC:Z:151M AS:i:106 XS:i:0 A00126:224:H7HMWDSX3:3:1108:21983:14794 97 Test_seq 130883 60 151M = 131101 345 GAGAGGGCAGAGGTGTCCAGAAGCTACCCAAATGGACACGAGGAGAGAGGGGGGAGGGAAGTACTGTGCTATTTGGTCAGAGGGAGAACAATTAGGAGTATATAGCAAGGTATATGTAAATTTTTGTATGAGAGACTGACCTGATTTGTAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:0 MD:Z:151 MC:Z:127M24S AS:i:151 XS:i:39 A00126:224:H7HMWDSX3:3:1108:21983:14794 145 Test_seq 131101 60 127M24S = 130883 -345 AAATAAGTTATCTGTGTAAGACCAAATGAACAACAGCTATTTTAAAGCTAGACAGGATGGTTGAGGGGCAGGGAGTTTAAGTCAATGGAAGTCAAACAACTAGAACAGAAATAACAAGAATGTTGACAATCACAAGGTCCTAGAAATGTCC ::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF NM:i:1 MD:Z:57A69 MC:Z:151M AS:i:122 XS:i:0 A00126:224:H7HMWDSX3:3:1108:30110:17973 81 Test_seq 212747 42 151M = 212594 -304 AGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:79A71 MC:Z:151M AS:i:146 XS:i:126 XA:Z:Test_seq,+168141,151M,5; A00126:224:H7HMWDSX3:3:1108:30110:17973 161 Test_seq 212594 60 151M = 212747 304 AGAAGTCCTAGCCAGGGCAATTAGGCTAGACAAAGAAATAAAGGGCATCTGGATTGCCAAGGAGGAAGTAAAATTATCTCTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATTCTCCAGAAAACTACTGAAACTAATA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:3G11A40G61T6C25 MC:Z:151M AS:i:127 XS:i:85 A00126:224:H7HMWDSX3:3:1108:7003:19069 97 Test_seq 168274 60 151M = 168563 440 GAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGACACCCTTTATTTCTTTGTCTAGCCTAA FFFFFFFFFFFF:,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF:FFFF:F:FFFFFFFFF NM:i:3 MD:Z:72G18A32T26 MC:Z:151M AS:i:136 XS:i:91 A00126:224:H7HMWDSX3:3:1108:7003:19069 145 Test_seq 168563 60 151M = 168274 -440 TGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGCGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGAT FFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFF:FFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:66G84 MC:Z:151M AS:i:146 XS:i:76 A00126:224:H7HMWDSX3:3:1108:3179:19805 113 Test_seq 167167 52 151M = 213767 46582 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA F:F:FFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:FFF:FF:FFFFF:FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:76T74 MC:Z:19S132M AS:i:146 XS:i:121 XA:Z:Test_seq,+213717,151M,6; A00126:224:H7HMWDSX3:3:1108:3179:19805 177 Test_seq 213767 35 19S132M = 167167 -46582 AAAATTTAAAAAAAAAAACAACCCATTCAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTTGATCATTAGCCATTAGAGAAATGCAAATTAAAACTATGATG FFFF,,,FFFFFFFFF:FFFFF,FF,F:FFFF:FF:F:FFFF:FFFFFFFFFFF,,F:F:FFFF:FFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFF NM:i:4 MD:Z:6A83C33G2C4 MC:Z:151M AS:i:114 XS:i:97 XA:Z:Test_seq,+167136,136M15S,8; A00126:224:H7HMWDSX3:3:1108:30544:19789 97 Test_seq 167316 7 22S129M = 167511 346 CCGTGGGTATTGATTGTGGATCGATCAGGCGCTGGTCTGAGATATCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGTTGTTTGATTTTTAGGAGCTCCCAGTTATCTGG :FFF:F:FFFF,F,FF:::FFF,F,FFF,FFF::FFFFFFFFFFFFFFFFFFFFFF,FF,:F:F,FFFFFFF:F,:FFFF:FFFFFF:FFFFFFF,F:FF:,FF,F:F:FFFFFFFFF,FF,FFF:FF:FFF:FF,FFFFF:F,F:FFF,F NM:i:5 MD:Z:7A13G23G6A43G32 MC:Z:151M AS:i:104 XS:i:99 XA:Z:Test_seq,-213590,129M22S,6; A00126:224:H7HMWDSX3:3:1108:30544:19789 145 Test_seq 167511 60 151M = 167316 -346 TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTCAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTG FFFF::FFFFFFFFF:F,F,FFFFFFFF,FFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFF::::FF:FFF:FFFFFF:FF:F:FFFFFFFFFFFFFF,FF::FFFF:FFFF:FFFFFFFFFFF::FFFFFFF::FFFFFFFFFFFF,FF: NM:i:1 MD:Z:48T102 MC:Z:22S129M AS:i:146 XS:i:117 XA:Z:Test_seq,+213377,83M2I23M2I37M4S,6; A00126:224:H7HMWDSX3:3:1108:8187:20400 65 Test_seq 77443 60 151M = 233514 156072 TCCTCAGACAGTGCAATTTTCCTTGGCTTTTCAGCCCATAAAATTACTTGATAAACACTGTCTGCTTCTAAGCTTTCGGCCTTACACAGTTGCTTTGCTCCATTGTAAATAATTAAGAATTTAACAGGCCACTGATTAAAAACAAAATTGG FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,,:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:::F:FFFFF:F,FFFF:F:FFFF:F:FF NM:i:1 MD:Z:42G108 MC:Z:27M124S AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1108:8187:20400 129 Test_seq 233514 48 27M124S = 77443 -156072 CTCAAACCCATTGCCGTCGAGTTGATTACTCCATATTGGCAAGTGCAGCAAGGCTGGGGCCCCTTCAGGACATATAGAATATAGCCAACATGAGGCCCAGGGTCAAGATCAGGGGGCCATTTGGTAGCCTCCAAAAGCAAAGACTAACATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFF::FFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF NM:i:0 MD:Z:27 MC:Z:151M AS:i:27 XS:i:0 SA:Z:Test_seq,77734,-,124M27S,60,0; A00126:224:H7HMWDSX3:3:1108:8187:20400 2193 Test_seq 77734 60 124M27H = 77443 -415 GATGTTAGTCTTTGCTTTTGGAGGCTACCAAATGGCCCCCTGATCTTGACCCTGGGCCTCATGTTGGCTATATTCTATATGTCCTGAAGGGGCCCCAGCCTTGCTGCACTTGCCAATATGGAGT FFFFF:FFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFF::FFFFFF:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFF NM:i:0 MD:Z:124 MC:Z:151M AS:i:124 XS:i:0 SA:Z:Test_seq,233514,+,27M124S,48,0; A00126:224:H7HMWDSX3:3:1108:8495:20494 97 Test_seq 162287 60 151M = 162422 261 GGAGGAGGCAGGCAGAGGGAGGAGAAAAGCTTATTGGCTTATCATGGCATTAGTCATGTCAAGCTCACAGACACCCATTTCGAAAAGGAGAAAACAAGCTGGGGAGTATTAGGGTCACATTCTGAAAGGTAGAGCCTCAAAAACCCTATGG FFF:F:F,FFFFFFF:FFF:FFF:FFFFFF,FFFF:FF:FFF:FF::F:FFFF:FFFFFFFFFF:F,FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFFFF::FFFFF,FF:FFFFFFFFFFFFFFFFFFF:,FFFFFFFFFF:FFFFFF NM:i:1 MD:Z:66C84 MC:Z:126M25S AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1108:8495:20494 145 Test_seq 162422 60 126M25S = 162287 -261 CTCAAAAACCCTATGGAAGAGTTCTTCTGTGCACACATGGGGTGGTCACGAGTCAGAATCGACTGGACTAGCAACTAACAACAGCAGGCAAGTTCGAAGGGTCCTCCTGGGTAGTTAGATGGGATCGATCTAATCTCTAAAATCTGTAGGA F:,F:F:FFFFFFFFFF,F:FFFFF:,FFFFFFF:F:FFFFFFFFFF,FF,:FF::,FFFF:FFFFFFFFFFFFFFFF:FFF:,FFF:F,FFFFFFFFFFFFF:FFFFFF,FFFFFFF:FFFFF:FFFF,,,FFFFFF:F::FFFFFFF:F NM:i:0 MD:Z:126 MC:Z:151M AS:i:126 XS:i:0 A00126:224:H7HMWDSX3:3:1108:7744:20509 97 Test_seq 169333 60 151M = 170144 835 GGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:F,FFFFF:F:FF: NM:i:1 MD:Z:134G16 MC:Z:127S24M AS:i:146 XS:i:109 A00126:224:H7HMWDSX3:3:1108:7744:20509 145 Test_seq 170144 30 127S24M = 169333 -835 CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCAATTGTCCCTTTTATCATTA FF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF NM:i:0 MD:Z:24 MC:Z:151M AS:i:24 XS:i:0 SA:Z:Test_seq,169626,-,121M30S,60,4; A00126:224:H7HMWDSX3:3:1108:7744:20509 2193 Test_seq 169626 60 121M30H = 169333 -414 CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTA FF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF NM:i:4 MD:Z:2T11A22G70A12 MC:Z:151M AS:i:103 XS:i:0 SA:Z:Test_seq,170144,-,127S24M,30,0; A00126:224:H7HMWDSX3:3:1108:16233:21339 81 Test_seq 197343 60 132M19S = 197163 -312 ATTCTAGGGGGAATTGCTTCCATGACAGTGTATTGAACCTGTGCTCCAAGGGGTGCCTTTAACAGTGGACTTGGTATCCTCTGGAGTTGTACAACGTAATTGCTCTGAGCCCAGAGCCCAGGAAATAAGATCGATCTTGATTTTCAGTACG FFF:,FF:FFFFFFF:FFFF:FFFFF:FFF:FFFFF:::FFFFFFF:F,FF:FFF::FF:FF,FFFFFF:FFFFFFFFFFFFFFFFFF:FFF::FF:FFFFFFFFFFFFFF::FFF:FF:FFF,FFFF:FFFFFFFFFF,FF:FFFFFFFF NM:i:0 MD:Z:132 MC:Z:151M AS:i:132 XS:i:0 A00126:224:H7HMWDSX3:3:1108:16233:21339 161 Test_seq 197163 60 151M = 197343 312 TCACATTTTTCTCCCAGCATACGCATGCTGATGTGGGGTTTTGTCATGTTTCTTAAGGGAACTGTGATGAAAATTCTCCTCTGACAGACATGTCTCAACTTCCAGATGGTAAGAAATTTTGTGAGAGTGATGAAATTAAGATACTTGATGC FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFF:F:FFFFFFFFFFFFFFF,FFFFFFFFFF:,F:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:132M19S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:16342:23437 97 Test_seq 171131 60 151M = 171294 291 GCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTATCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFF::FFFF,FF,:FFF,FFFFFFFFFFFFF:FFFFFFFF,FF:F:FFF,FFFFF:FFFFF:FF: NM:i:1 MD:Z:71C79 MC:Z:128M23S AS:i:146 XS:i:75 A00126:224:H7HMWDSX3:3:1108:16342:23437 145 Test_seq 171294 60 128M23S = 171131 -291 TAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTTAATGGATGTGTGCAGCTTTTCT :FFF:,FFFFFFFFFF:FFF,FFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FF:FF:FFF:FFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFF:FFFFF:FFFFF NM:i:2 MD:Z:106G15T5 MC:Z:151M AS:i:118 XS:i:43 A00126:224:H7HMWDSX3:3:1108:16740:23625 81 Test_seq 170680 60 151M = 170381 -450 GCGTTCTATATCACCTTGTATCACTCTCCATCTGGTAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGTTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAA F:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:4 MD:Z:0T4G29C15G99 MC:Z:151M AS:i:135 XS:i:0 A00126:224:H7HMWDSX3:3:1108:16740:23625 161 Test_seq 170381 60 151M = 170680 450 CTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGCGACGTTTTTC F:FFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::F:FFFFF:F,FF:FFFFFFF:FF: NM:i:1 MD:Z:140A10 MC:Z:151M AS:i:146 XS:i:47 A00126:224:H7HMWDSX3:3:1108:25789:25488 81 Test_seq 239390 60 122M29S = 239267 -245 TTTTCAATGGCTGCTTTTTCAGAAGTAGATAGACAAGTCTTTCTTCGACGTGCTTCTGGGTCAACTTGTTACCAAACCGAACTCGGGCCCCTCCCTGCCCGGTGCCTGCAGAGTTCCAATTAGATCTTCAGGTTTTGAATCACAGAACATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:0 MD:Z:122 MC:Z:151M AS:i:122 XS:i:0 A00126:224:H7HMWDSX3:3:1108:25789:25488 161 Test_seq 239267 60 151M = 239390 245 CATTTTTAAATGATGTCAAATTTATTTATGGATATTGAATACAATAAAAATCAGTTGCCATCCAGTAGATTTCTACTCATGGCGATCCACATACGTCACAGTAGAACTGTGTTCCATGGGGTTTTTTCAATGGCTGCTTTTTCAGAAGTAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:122M29S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:25491:25661 97 Test_seq 240923 60 151M = 241150 378 GATGAGCTCAAGCATAACGATTGTAAGGATGGCACAGGACTGGGCAGTGTTTCGTTCTGTTGTGCATAGGGTCGCTATGAGTTGGTACCGACTGGACAGCACCTAACAACAACAACAATGGAGAAGTTCAAACAAAGACAAAATGGGCCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,FF::FF::,F,F,F:,FF:FFFFF:F,FFFFF:FF:FFF:F,,F,F,F::F,FFF:FF,FF::F:F,,FFFF,: NM:i:2 MD:Z:85A49T15 MC:Z:151M AS:i:141 XS:i:59 A00126:224:H7HMWDSX3:3:1108:25491:25661 145 Test_seq 241150 60 151M = 240923 -378 CTAAAATTTTATGACTCTGGACTATGTTTGACCTGCTTGAAGAAAATGTTCTAATCAATAATCCTTGTCACTTTCCAAAATTTAAGTCCTTGTTCTGGAAGAGGCTTAAACTGAGAAGATAAAGGGGGAAAAAATCAGTACCAAACCAGGT FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:26033:26694 81 Test_seq 170371 60 151M = 170197 -325 CAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGA FFFFFFFFF,FFFF,FFF:FFFFFFFF,FFFFFFFFFFF:FFF:FF::FFFF,F:FFFFFFFFFFFFF,F,F,FFFFFFFFFFFFF,FFFFF:FFF:,FFFFFFFFF:F:FFFFFFF:,F:FF:FFFFFFF,F,FFFF:FF:FFFFFFFFF NM:i:1 MD:Z:119T31 MC:Z:4S147M AS:i:146 XS:i:48 A00126:224:H7HMWDSX3:3:1108:26033:26694 161 Test_seq 170197 60 4S147M = 170371 325 GGTGGATTTAAGTCTAAAGTCTATTTTTTCAGAAATTAATATTGCTACTCTTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAG FFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFF:F:FF:FFFF:FFFFFFFFF::FFFFFFFFFF::FFF,FFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF:FFFF:FF,FFF:FFFFFF:,FF:F:F,FFFFF:FFFF,F NM:i:2 MD:Z:23G22C100 MC:Z:151M AS:i:137 XS:i:69 A00126:224:H7HMWDSX3:3:1108:3251:26850 113 Test_seq 214824 26 54M = 166158 -48667 AAGAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTGA :F,:,FFFFFFF,FFFFF:F,FFF:FFFF:FFF:FFF:FFFF,,FFFFFFFFFF NM:i:1 MD:Z:1T52 MC:Z:54M AS:i:52 XS:i:44 XA:Z:Test_seq,+166158,54M,2; A00126:224:H7HMWDSX3:3:1108:3251:26850 177 Test_seq 166158 25 54M = 214824 48667 TCAGTATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTT FFFF::F,FF,FFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:4G49 MC:Z:54M AS:i:49 XS:i:41 XA:Z:Test_seq,+214829,5S49M,2; A00126:224:H7HMWDSX3:3:1108:3378:27226 97 Test_seq 72787 60 151M = 73058 422 AAGGAGAAAAAAGGAAATATTTGTACCCTGTATGCAAATATCTTGGAATGGAGCAGTAGTCTGCAGTAATGGGGGAACTGAAAAATTCCTAGAAGCTGTCAGCCTGAAATGAATATTTTATAAACATATATGATTTGGAGGCTTATATAAT FFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFF::FFFFFFF:FFFFFFF,FFFFFFFFFFFFF:F:FFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFF:FFFFFF,FFF,FFFF:FFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:3378:27226 145 Test_seq 73058 60 151M = 72787 -422 AGGTTCTCACATGGATTCTCATGAAAATTATATGAGCACGTTGTATTAATTTGTTTAAAAAAAAAATTGGTACTTTTTTATTGGCTTTGCTTCTTTTGGTTCTTACTGCCTATATCAGAAGACGTTGTATTCATTTAAAGGAAGAGCATTC F,FF,FF:FFFFF,:FF,FFFF,FF::FFFFFFFFF:FF:FFFFFFFF:FFFF,FFFF:FFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFF,FFFFFFFF NM:i:2 MD:Z:4G48G97 MC:Z:151M AS:i:141 XS:i:0 A00126:224:H7HMWDSX3:3:1108:13639:27524 81 Test_seq 330936 60 151M = 330730 -357 AACTCATGGCAACTCTAGGAAACAGAATGGGTATGGCTTTATTGGAGTCCTTAAATTCTATGGCTGGCCTGCATCGTTCTCATTGATCCTAGCCCTATGTAATCAGGTTCCTCTTCCTACCCAGCTGCTGGCTCTTCCTGTGTTTGTTAGG FFFFF,FFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:13639:27524 161 Test_seq 330730 60 151M = 330936 357 AAAAGGTCAATCAAGTAGCCAGGAGTCCCAGGTGGCAGGGCTGGTTAACTGGAATATTAAGTCACTGAGTTTCCAATACAACTCTTGGCCCAACAACCAAAAATACCTATTTCATTTTTGCTTCTGGGTGAATTTCAGGAGTTGGGGGTTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:F NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1108:26223:27837 81 Test_seq 170371 60 151M = 170197 -325 CAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:119T31 MC:Z:4S147M AS:i:146 XS:i:48 A00126:224:H7HMWDSX3:3:1108:26223:27837 161 Test_seq 170197 60 4S147M = 170371 325 GGTGGATTTAAGTCTAAAGTCTATTTTTTCAGAAATTAATATTGCTACTCTTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFF::F:FFFF,FFFFFFFFF,FFFF:FFFFFFF:FFFFFFF:FFFF NM:i:2 MD:Z:23G22C100 MC:Z:151M AS:i:137 XS:i:69 A00126:224:H7HMWDSX3:3:1108:14561:28056 97 Test_seq 168438 60 151M = 168550 263 GACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTT F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:1 MD:Z:57T93 MC:Z:151M AS:i:146 XS:i:86 A00126:224:H7HMWDSX3:3:1108:14561:28056 145 Test_seq 168550 60 151M = 168438 -263 GGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATGAATGGGTGTTAGACTTTGTCAAATGCCTTTTCTGCATCAGTTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTAT FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFF::FFFF NM:i:6 MD:Z:74A10G0A26T0A22A13 MC:Z:151M AS:i:121 XS:i:81 A00126:224:H7HMWDSX3:3:1108:9968:28338 81 Test_seq 170323 12 51M = 170323 -51 AGGTGTGTCTCTTGTAGGCAGCATATAGACAGATCGTGTTTCTTTATCCAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:29T0G20 MC:Z:51M AS:i:41 XS:i:36 XA:Z:Test_seq,+38373,15S36M,0; A00126:224:H7HMWDSX3:3:1108:9968:28338 161 Test_seq 170323 60 51M = 170323 51 AGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAG FFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFF NM:i:0 MD:Z:51 MC:Z:51M AS:i:51 XS:i:29 A00126:224:H7HMWDSX3:3:1108:5285:30373 97 Test_seq 167167 52 151M = 167401 369 GCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFF,:F:FFFF:F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:76T74 MC:Z:135M16S AS:i:146 XS:i:121 XA:Z:Test_seq,-213717,151M,6; A00126:224:H7HMWDSX3:3:1108:5285:30373 145 Test_seq 167401 19 135M16S = 167167 -369 GATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCGATCTAGAAAAAAAAT FFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFF,FFFFFFFFFFF NM:i:5 MD:Z:86G2C22A0T2T18 MC:Z:151M AS:i:110 XS:i:100 XA:Z:Test_seq,+213499,16S135M,7; A00126:224:H7HMWDSX3:3:1108:23592:31234 81 Test_seq 405676 60 127M24S = 405523 -280 GCATGAACATGGATCTCTTCTAGTCAGTTGCAGATCCTTGTAATTTCCACTGTTACCTATCTTTACCCCCCACTTACTTATAAAGTTTCTAGTTTTGCAGATTGTTCAGTTGTTGTTGTTGTTTTTATAAAGCAAAAATGTCATCAATGTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:127 MC:Z:151M AS:i:127 XS:i:0 A00126:224:H7HMWDSX3:3:1108:23592:31234 161 Test_seq 405523 60 151M = 405676 280 CCTTTTCCGTAGTTTGTTATTGCGGTAGTTCCCTGTTGATGTACTGCTGTAACTGGTTTTGAATTATCTTCAGCAAAATTTTACTTGCATGTGATATTGCTAGTATCGTGCGGTACTCTTCACATTCTGTCGGATCTCCTTTCTTTGGAAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:101G49 MC:Z:127M24S AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1108:15745:32706 97 Test_seq 213581 32 151M = 213774 344 GAAGAGAAACCAGATAACTGGGAGCTCCTAAAAATCAAACACCTATGCTCATCTGAAGACTTCACCAAAAGAGTAAAAAGACGACCTACAGATTGGGAAAGAATTTTCAGCTATGACATCTCCGACCAGCGCCTCATCTCTAAAATCTATA FF:FFFFFFFFFF:F::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFF:FFFFFFFFF:FFF:FFFFFFFFFFFF:F,F:FF:FFFF NM:i:5 MD:Z:54A27C5T3C41G16 MC:Z:151M AS:i:126 XS:i:109 XA:Z:Test_seq,-167303,151M,9;Test_seq,-400724,136M15S,7; A00126:224:H7HMWDSX3:3:1108:15745:32706 145 Test_seq 213774 60 151M = 213581 -344 TCAAGAAGTGGGCAAAGGATATGAACACGCACTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCT FFFFFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:117G33 MC:Z:151M AS:i:146 XS:i:115 A00126:224:H7HMWDSX3:3:1108:30246:32957 81 Test_seq 170621 60 9S142M = 170315 -448 GTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAATCTAACTTGTATTGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCAATGCCTCCTGTATTTAGTCCCTCTTTTTGATTATTTTGATC :FFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:48C11C3G6A18C45G5 MC:Z:151M AS:i:112 XS:i:37 A00126:224:H7HMWDSX3:3:1108:30246:32957 161 Test_seq 170315 60 151M = 170621 448 TAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFF NM:i:1 MD:Z:61C89 MC:Z:9S142M AS:i:146 XS:i:48 A00126:224:H7HMWDSX3:3:1108:3079:34131 65 Test_seq 18385 13 51M = 214177 195793 ATCCATTCATCCATTGATGGGCACCTTGGTTGCTTCCATCTTTTTGCTATT FFFFFFFFFFFFFFFFFFFF,FF,FFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:0 MD:Z:51 MC:Z:51M AS:i:51 XS:i:46 XA:Z:Test_seq,+48772,51M,1;Test_seq,-41848,51M,1; A00126:224:H7HMWDSX3:3:1108:3079:34131 129 Test_seq 214177 3 51M = 18385 -195793 AATAGCAAAAAGCTGGAAGCAACCAAGGTGTCTATCAACGGATGAATGGAT FFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFF:F:FFFFFFFFF::F NM:i:3 MD:Z:32C5T10T1 MC:Z:51M AS:i:39 XS:i:36 XA:Z:Test_seq,-48772,51M,3;Test_seq,+41848,51M,3;Test_seq,-400228,51M,4; A00126:224:H7HMWDSX3:3:1108:19533:34569 113 Test_seq 168440 60 151M = 212738 44299 CTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:55T95 MC:Z:151M AS:i:146 XS:i:88 A00126:224:H7HMWDSX3:3:1108:19533:34569 177 Test_seq 212738 0 151M = 168440 -44299 ACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACACCGAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTTAGGA FFFFFFF,FFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:36G49T1A10A51 MC:Z:151M AS:i:131 XS:i:131 XA:Z:Test_seq,+168150,151M,4; A00126:224:H7HMWDSX3:3:1108:20112:34757 97 Test_seq 168195 8 25S126M = 168454 410 TACATCCTTGGTAAGATTTATTCCTTTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACATGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGA FFFFFF::F:FFFFF:FFFFFFFFFFFFF:FFF::FF,F:FFFF,::FFFFFF:FFFFFFFFFFFF,FFFFF:FFFF:FF,,FFFFFFF:FFFFFFFFFFFF,FFF:FFFFFFF,FFF,F:FFFFFF,FF:F:FFFFFF:FFF:FF:FF,F NM:i:3 MD:Z:76T0C9T38 MC:Z:151M AS:i:111 XS:i:106 XA:Z:Test_seq,-212718,126M25S,4; A00126:224:H7HMWDSX3:3:1108:20112:34757 145 Test_seq 168454 60 151M = 168195 -410 TTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTT F:FF,F,FFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFF,F,FFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFF::FFFFFF:FF:FFFF::FF:FF,FFFFFF:FFF:FF,FFFFFF:FF:FF,FF: NM:i:1 MD:Z:41T109 MC:Z:25S126M AS:i:146 XS:i:90 A00126:224:H7HMWDSX3:3:1108:20374:35243 97 Test_seq 168195 8 25S126M = 168454 410 TACATCCTTGGTAAGATTTATTCCTTTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGA FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:F NM:i:2 MD:Z:76T10T38 MC:Z:151M AS:i:116 XS:i:111 XA:Z:Test_seq,-212718,126M25S,3; A00126:224:H7HMWDSX3:3:1108:20374:35243 145 Test_seq 168454 60 151M = 168195 -410 TTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTT FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:41T109 MC:Z:25S126M AS:i:146 XS:i:90 A00126:224:H7HMWDSX3:3:1108:22788:35509 65 Test_seq 251240 0 23M128S = 168739 -82502 GTCCTATAGGGTTGCTATGAGTTAACACATTCAGAAGTAGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAACAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCT FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFF:,F:FFFFFFF:FFFF:F:FFFFFF NM:i:0 MD:Z:23 MC:Z:151M AS:i:23 XS:i:23 SA:Z:Test_seq,169010,-,129M22S,60,1; XA:Z:Test_seq,-310625,128S23M,0;Test_seq,+365190,22M129S,0; A00126:224:H7HMWDSX3:3:1108:22788:35509 2129 Test_seq 169010 60 129M22H = 168739 -400 AGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTGTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTA FFFFFF:F:FFFF:FFFFFFF:F,:FFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF: NM:i:1 MD:Z:47T81 MC:Z:151M AS:i:124 XS:i:53 SA:Z:Test_seq,251240,+,23M128S,0,0; A00126:224:H7HMWDSX3:3:1108:22788:35509 129 Test_seq 168739 60 151M = 251240 82502 AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGTATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAA FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:F:FFFF:::,FFFFFFFFFFFFFFFFFFF:FF,FFFFFFF,:FFFFFFFFFFF,FFFFFFFFFFFFF:F:FFFFF:FF,FFFFFFF:F:,FFFFFFFFFFFF,F:F,FFFFFFFFFF, NM:i:1 MD:Z:44A106 MC:Z:23M128S AS:i:146 XS:i:81 A00126:224:H7HMWDSX3:3:1108:32416:35650 81 Test_seq 381026 60 151M = 379713 -1464 GCGTATTTGAGAAGCAGTATTACAAATCGCTGAGTATTAAATGTCCTTCTGGTTGTTTGTATTTGTCTTCCTTTCCCTTTGTCCATGGTTATCCATGCCTACGGAGGGAGAGCAGGAATGTATAATACAAAATCCTGCGGTAATCTGTGCG F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FF:,FFFFFFFFFFF:FFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:116C34 MC:Z:26M125S AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1108:32416:35650 161 Test_seq 379713 42 26M125S = 381026 1464 GTGCTCCTTCCGCCTGCCTTCTGATCAATCTGGCTCAGGTGCATGCTCTTAGCCGTTATGCTACATTGAAGGCAGGAGGATCAGGTAGATAAAGACAGAGGAGGAAAGTTTGGGGAGCGTATTTGAGAAGCAGTATTACAAATCGCTGAGT FFFFFFFFFFFFFFFFFF,FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFF:F::FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:0 MD:Z:26 MC:Z:151M AS:i:26 XS:i:0 SA:Z:Test_seq,380936,+,26S125M,60,0; A00126:224:H7HMWDSX3:3:1108:32416:35650 2209 Test_seq 380936 60 26H125M = 381026 241 AATCTGGCTCAGGTGCATGCTCTTAGCCGTTATGCTACATTGAAGGCAGGAGGATCAGGTAGATAAAGACAGAGGAGGAAAGTTTGGGGAGCGTATTTGAGAAGCAGTATTACAAATCGCTGAGT FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFF:F::FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:0 MD:Z:125 MC:Z:151M AS:i:125 XS:i:0 SA:Z:Test_seq,379713,+,26M125S,42,0; A00126:224:H7HMWDSX3:3:1108:7591:35837 81 Test_seq 170384 60 151M = 170207 -328 TCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTTATGTGTGTTGTTGACAATTTCATTTTTCCACATACTTTTTTGTGCCGAGGCGTCTTTCTTA FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:8 MD:Z:40C48C13G1C0T28T3A3T7 MC:Z:151M AS:i:111 XS:i:51 A00126:224:H7HMWDSX3:3:1108:7591:35837 161 Test_seq 170207 60 151M = 170384 328 TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:55A95 MC:Z:151M AS:i:146 XS:i:75 A00126:224:H7HMWDSX3:3:1108:10122:36902 81 Test_seq 167552 34 151M = 167329 -374 TTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCC FFFFFFFFF:F:FFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:20S131M AS:i:151 XS:i:135 XA:Z:Test_seq,+213336,124M2I25M,4; A00126:224:H7HMWDSX3:3:1108:10122:36902 161 Test_seq 167329 21 20S131M = 167552 374 GCGATGTACTTCAGGGTAGATCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGGCATTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGCTTTCTCTTCATCATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FF,:FFFF:FFFFFFFFFFFFF:FFFFFFF:FFFF:F:F,:F:FFFF:FFFFF NM:i:5 MD:Z:32G6A4T1T68G15 MC:Z:151M AS:i:106 XS:i:95 XA:Z:Test_seq,-213575,128M23S,7; A00126:224:H7HMWDSX3:3:1108:8693:36996 81 Test_seq 167552 34 151M = 167329 -374 TTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:20S131M AS:i:151 XS:i:135 XA:Z:Test_seq,+213336,124M2I25M,4; A00126:224:H7HMWDSX3:3:1108:8693:36996 161 Test_seq 167329 21 20S131M = 167552 374 GCGATGTACTTCAGGGTAGATCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGGCATTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGCTTTCTCTTCATCATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:32G6A4T1T68G15 MC:Z:151M AS:i:106 XS:i:95 XA:Z:Test_seq,-213575,128M23S,7; A00126:224:H7HMWDSX3:3:1109:6036:1517 97 Test_seq 168277 60 27S124M = 168520 394 TTAATATAGTCACTGAGGTATTTATTAACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGCGATAGCTTTACTTCCTCTTTACCAATCTGGATAC FFFFFFFFF,:FFFFFFFFFF:FFFFFFFFFFFF,FF,:FFFFFFFFFFFF:FFFFFFFF:FFFFFFFF:FFF:F:FF:FFFFFFFFFFF,FFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFF,:FFFF:FFF,FFFFFFFFF::FFFF NM:i:5 MD:Z:69G18A0T17C2G13 MC:Z:151M AS:i:99 XS:i:67 A00126:224:H7HMWDSX3:3:1109:6036:1517 145 Test_seq 168520 60 151M = 168277 -394 TTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTATTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATA FFFF:F:FF:FFF:FFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFF,FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:112G38 MC:Z:27S124M AS:i:146 XS:i:61 A00126:224:H7HMWDSX3:3:1109:25039:2895 81 Test_seq 171336 60 151M = 171148 -339 CTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCACCTATTTTCTCTGTGTTTTCTCTCCTTCCTCCCTGTTCTGGGTCTCCAATCACTTGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTT FFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF NM:i:8 MD:Z:41A20G1G1C0C12T2A11C55 MC:Z:151M AS:i:111 XS:i:48 A00126:224:H7HMWDSX3:3:1109:25039:2895 161 Test_seq 171148 60 151M = 171336 339 TGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTATCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,F:FFFF:FFFFFFFFFFFF::FFFF:F:FFF::FFFFFFF:FFFFFFFFFFFF::FFFF:FFF: NM:i:1 MD:Z:84G66 MC:Z:151M AS:i:146 XS:i:39 A00126:224:H7HMWDSX3:3:1109:20627:4805 81 Test_seq 395896 10 66M = 116647 -279315 CCATAATTTGTTATGATCCACACAGTCGAATACCTTTGTATAGTCAATAAAACACAGGTAAACATT FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:27A10C26C0 MC:Z:66M AS:i:55 XS:i:50 XA:Z:Test_seq,+384014,66M,4;Test_seq,-47037,3S63M,4;Test_seq,-316208,51M2I13M,5;Test_seq,-15119,66M,5;Test_seq,-116647,66M,5; A00126:224:H7HMWDSX3:3:1109:20627:4805 161 Test_seq 116647 12 66M = 395896 279315 CCATAATTTGTTATGATCCACACAGTCGAATGCCTTTGCATAGTCAGTAAAACACAGGTAAACATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF, NM:i:1 MD:Z:28G37 MC:Z:66M AS:i:61 XS:i:56 XA:Z:Test_seq,-384014,66M,2;Test_seq,+15119,66M,3;Test_seq,+395896,66M,3;Test_seq,+316208,51M2I13M,3; A00126:224:H7HMWDSX3:3:1109:27425:6778 97 Test_seq 167324 6 151M = 167581 408 GCTGGTCGGAGATGTCATAGCTGAATATTCTTTCCCAAACTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGACCTCCCAGTGATCTGCTTTCTCTTCATCATTTTTGGTAATGTTTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FF:FFFFFFFF:FFFFFFFFFF::FFF:F,FFFFFFFFFFFF:FFFFFFFF:,,::FF,,FF:FFFFF:FFFFFF:FFFFF:FF:F,FF NM:i:7 MD:Z:7T29G0T5A60G8T5G30 MC:Z:151M AS:i:116 XS:i:111 XA:Z:Test_seq,-213560,151M,8; A00126:224:H7HMWDSX3:3:1109:27425:6778 145 Test_seq 167581 7 151M = 167324 -408 TTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCTGCTCATACATGG FFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:145T5 MC:Z:151M AS:i:146 XS:i:141 XA:Z:Test_seq,+213307,151M,2;Test_seq,+40976,151M,7; A00126:224:H7HMWDSX3:3:1109:11858:6809 97 Test_seq 168496 60 151M = 168685 315 GTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:::FFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:140A10 MC:Z:126M25S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1109:11858:6809 145 Test_seq 168685 60 126M25S = 168496 -315 TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTACTCCCCAGTACAACTCTCTGCTAA FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:2A28T94 MC:Z:151M AS:i:118 XS:i:58 A00126:224:H7HMWDSX3:3:1109:3052:8061 81 Test_seq 168570 60 151M = 168347 -374 TATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTA FFFFFFFFFFF,FF:FFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:146T4 MC:Z:25S126M AS:i:146 XS:i:74 A00126:224:H7HMWDSX3:3:1109:3052:8061 161 Test_seq 168347 60 25S126M = 168570 374 AAATATTGCCAAATAATCAGATCGATCATGTCATCAGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGATGAATAAGAGTGGTGATA :FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFF,FF,FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF,FFFFFFFFFFF:,FFFFFF,FF,FFFF:FFF,F:F:FFF:FFFF,,FF,FFF,::FFFFFFFF::FFFF:F NM:i:4 MD:Z:10T7A88T10C7 MC:Z:151M AS:i:106 XS:i:56 A00126:224:H7HMWDSX3:3:1109:31213:9298 81 Test_seq 171635 60 151M = 171470 -316 TGGATTTCTACATGCTGACTCTCTATGGATTCTTGCAACTTATTAATTTTTCCGCTATGTTCTTGAATAACCTTTTTGAGTTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTT FFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFF,FFFFFFFFFFF NM:i:4 MD:Z:17T35A2C25T68 MC:Z:151M AS:i:131 XS:i:58 A00126:224:H7HMWDSX3:3:1109:31213:9298 161 Test_seq 171470 60 151M = 171635 316 AGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTT FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFF NM:i:1 MD:Z:113T37 MC:Z:151M AS:i:146 XS:i:34 A00126:224:H7HMWDSX3:3:1109:20907:9392 81 Test_seq 123962 60 133M18S = 123707 -388 GGATCACCGTGGGTTGGAATCCACTCAGTGACAACTGGCGTTTGTGCTAGTCACCTCGAGAAGAGTCTGTTACTCCTTGTCCTTAGCTGGGTCAGATAACCATGGCCAGGTGTTGACTGATACGTAAAGTTAATCTGGAATGTACGAAGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:133 MC:Z:151M AS:i:133 XS:i:0 A00126:224:H7HMWDSX3:3:1109:20907:9392 161 Test_seq 123707 60 151M = 123962 388 TAGGAACCCTATGGGGTAGTTCCACTGTGTCCTGTGGGGTCACTATGATTCAGAAGAGACTCGACACCACTGGTTTTGATTTTTTGGTAGTTACCCTCAGTCCCAGGGTGGTGTAAATGGTTAAGCACTTGGCTGCTAACTGAAAAGTTGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:F,,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:0 MD:Z:151 MC:Z:133M18S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1109:16369:10802 97 Test_seq 214055 60 151M = 214214 290 CAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGT FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFF NM:i:1 MD:Z:0A150 MC:Z:131M20S AS:i:150 XS:i:119 A00126:224:H7HMWDSX3:3:1109:16369:10802 145 Test_seq 214214 1 131M20S = 214055 -290 ATGGATTAATGGATAAATAAATTGTGGTATATTCACACAATGGAATACTACACATCGATAAAGAACAGTGACGAATCTGTGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGCGAAATGAGTAAACTCATTAGAATGG F::,F:,FFFF:FFFFF:FFFFFFF:F:FFFFFFFFFFFF,,:FFFFF:FFFFFFFFF:FFF:FFFF:::FFFFFFFFF,FFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF NM:i:6 MD:Z:6G5T37T0G4A68T5 MC:Z:151M AS:i:101 XS:i:98 XA:Z:Test_seq,+166686,16S135M,8;Test_seq,+48661,26S125M,6; A00126:224:H7HMWDSX3:3:1109:5321:13714 97 Test_seq 171138 60 151M = 171427 440 TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATGATATGTCTTGGTGTTTTTCTTTTTGGATC FFFFFFFFFFFFFFFFFFFFFFF,:FFF:FFFFFFFFFFFFFF:FFFFFFFF,F,::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,F,:,FFFFFFF:FFFFFFFFFFFFFFFFF:FFF:FFFFFF:FFF,FFFFFFFFF NM:i:1 MD:Z:121A29 MC:Z:151M AS:i:146 XS:i:60 A00126:224:H7HMWDSX3:3:1109:5321:13714 145 Test_seq 171427 60 151M = 171138 -440 CACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGTTTCCGTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGT ,FFFFFFFFFFFFFF,FFFFFFFFFFFFF,FFFFFFFF:FFFF,:F,FFF:FFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFF:FFFF NM:i:3 MD:Z:98A4A4C42 MC:Z:151M AS:i:136 XS:i:43 A00126:224:H7HMWDSX3:3:1109:8666:14121 97 Test_seq 171003 60 151M = 171122 270 ACTTGTGTTTATCTGTAAATATCTTAATTTCTCCTTCATATTTCAAAGAGAGTTTTGCTGGATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATATGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTA FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFF NM:i:4 MD:Z:44C0G42A19C42 MC:Z:151M AS:i:131 XS:i:79 A00126:224:H7HMWDSX3:3:1109:8666:14121 145 Test_seq 171122 60 151M = 171003 -270 TCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATAGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:50T100 MC:Z:151M AS:i:146 XS:i:68 A00126:224:H7HMWDSX3:3:1109:8178:14873 97 Test_seq 168739 60 151M = 168918 330 AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF,FFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFF:FFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:81 A00126:224:H7HMWDSX3:3:1109:8178:14873 145 Test_seq 168918 60 151M = 168739 -330 GATGGTGGCTTCATAGAATGAGTTGGGTAGTATTCCGTCTTTTACTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAGGTCTTCTCTAAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:41G1T36A9G60 MC:Z:151M AS:i:131 XS:i:66 A00126:224:H7HMWDSX3:3:1109:21784:15233 81 Test_seq 363814 60 151M = 363608 -357 GAAAGCCTGATGCCAGAAGTCTATTCTTTGAAATAGCAAGAGTTAAGAGTACCTGGTAAAGCTCTACCCACCAGCCTCTGTGACTGTCTTTCTTTGTTAAAGGTACATTCTGGTCTGCATTTTGCAGCGAGTCTTCAGGTAAGCAGGAGAG :F,FF,FFF:F:F,:FFF,FF:,FF,FFFFF,FFFF:,F,FFFF::F:,FF:FFFFFFFF:FFFFFF:F,::F,FFF:FF:FFFFF::FFF:FFFFF,FF:FFF,FFFFFF,FFFFFF::FF:FFF,FFF:FFFFFFFFF:F,FFFFF:F, NM:i:1 MD:Z:22C128 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1109:21784:15233 161 Test_seq 363608 60 151M = 363814 357 TAATAACACCAATAATATTCCAAAACACATATCCATATTTAGAAGTTAGATATCCATTATCAGCTTAATCAGTTCTATGTAATTCATTCTTGCTTCTCCCTCATTACCTTGATTTCATGAAATCATCTGTTTCTTGACAAAAGAGTTTTGG ,FFFFFFFFFFFFF::F:FFFFFF::,:F:F:,FF:F:,FFFFFF,FF:F,,FF:F::FF:FFFFF:F,FFF,F:FFFF:F::,FFFFFF::FF:FF,F:F,FF,FFF:F:FF:,,F,FFFFFFF,,F,,,FFF,,F:,FF::F,F,FF,: NM:i:4 MD:Z:45G5T82G3T12 MC:Z:151M AS:i:131 XS:i:0 A00126:224:H7HMWDSX3:3:1109:12816:15640 97 Test_seq 166376 60 151M = 166514 268 TTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCACTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFF:::F,FFFF:F:FF:FFF NM:i:1 MD:Z:47G103 MC:Z:130M21S AS:i:146 XS:i:30 A00126:224:H7HMWDSX3:3:1109:12816:15640 145 Test_seq 166514 60 130M21S = 166376 -268 TTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCAATAAAAAACCCTCTCCCTCCCTCCCTTCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTTCTATTTCTCAAGATCGATCAGGAACCAGACAAGGAT FFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:67C56C5 MC:Z:151M AS:i:120 XS:i:0 A00126:224:H7HMWDSX3:3:1109:24198:16438 97 Test_seq 46648 0 62M7S = 137021 90442 CCAGTTGTGAGGATTTTTGTTTTCTTTATGCTGAGGTATAATCCATACTGAAGGCTGTGGTCATTAATC FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF NM:i:2 MD:Z:8A28G24 MC:Z:69M AS:i:52 XS:i:52 A00126:224:H7HMWDSX3:3:1109:24198:16438 145 Test_seq 137021 8 69M = 46648 -90442 CCAGTTGTGAGGATTTTTGTTTTCTTTATGATGAGGTGTAATCCATACTGAAGGCTGTGGTCTTTGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:30T38 MC:Z:62M7S AS:i:64 XS:i:59 XA:Z:Test_seq,-315822,69M,2;Test_seq,-395506,69M,2;Test_seq,-333097,69M,3;Test_seq,-46648,69M,4;Test_seq,-8650,69M,4; A00126:224:H7HMWDSX3:3:1109:21965:18239 65 Test_seq 168498 60 79M = 40140 -128359 TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATG F,FFF::FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FF:,:FFFFFFFFFF,F,FFFFFFFFFFFFF,F:F:FFF NM:i:0 MD:Z:79 MC:Z:79M AS:i:79 XS:i:49 A00126:224:H7HMWDSX3:3:1109:21965:18239 129 Test_seq 40140 51 79M = 168498 128359 CATAATAGAGGGCATTTATACAAAGCCAACAGCCAACATCATCCTAAATGGAGAGAGCCTGAAAGCAATTCCCTTGAGA FFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFF:FFFFFFFFFF:FFFFF:F:FFFFF,F:FFFFFFFFF NM:i:2 MD:Z:7A59T11 MC:Z:79M AS:i:69 XS:i:49 A00126:224:H7HMWDSX3:3:1109:32081:19257 81 Test_seq 170494 60 151M = 170202 -443 CATTTTTCCACATGCTTTTTTGTGCTGAGACGTTTTTCTTAGTAGCTTGTGAGATCCTCATTTTCATAATGTTTAACTTTGTGTTTGTTGAGTCGTTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATT FFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFF,FF NM:i:3 MD:Z:13A31A34A70 MC:Z:151M AS:i:136 XS:i:20 A00126:224:H7HMWDSX3:3:1109:32081:19257 161 Test_seq 170202 60 151M = 170494 443 AAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGAT F:FFFFFFFF:FFF,FFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:FFFFFF:FFFFF:FF:FFFFFFFF:FF,FFFF:FFFF:FFFFFFFFF,FF,FF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFF NM:i:1 MD:Z:60A90 MC:Z:151M AS:i:146 XS:i:88 A00126:224:H7HMWDSX3:3:1109:24975:20415 81 Test_seq 166330 60 151M = 166044 -437 GTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTAGAAGTCCCCTCAAGCCAAGTTCCCCAGACCCCAGCCCCTGCTTCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTACTTTTAATCCAGTCCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:9 MD:Z:12A18A8G14T6A3T24C42G6G9 MC:Z:151M AS:i:106 XS:i:27 A00126:224:H7HMWDSX3:3:1109:24975:20415 161 Test_seq 166044 60 151M = 166330 437 TGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTT FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFF, NM:i:1 MD:Z:90G60 MC:Z:151M AS:i:146 XS:i:44 A00126:224:H7HMWDSX3:3:1109:8169:20619 81 Test_seq 168660 60 151M = 168399 -412 ATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:56T94 MC:Z:151M AS:i:146 XS:i:55 A00126:224:H7HMWDSX3:3:1109:8169:20619 161 Test_seq 168399 60 151M = 168660 412 ACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTACGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCCGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:38G57T50T3 MC:Z:151M AS:i:137 XS:i:71 A00126:224:H7HMWDSX3:3:1109:31367:22592 65 Test_seq 213168 7 151M = 167451 -45718 AAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGC F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:144A6 MC:Z:81M4I66M AS:i:146 XS:i:141 XA:Z:Test_seq,-167720,151M,2; A00126:224:H7HMWDSX3:3:1109:31367:22592 129 Test_seq 167451 54 81M4I66M = 213168 45718 TTCATCATTTTTGGTAATGTTTTGTGTTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATGCACTTGGAGTTAGTTTTTGTGCATGG FFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFF:FFFFFF,F:FFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:8 MD:Z:25A36A0T56C26 MC:Z:151M AS:i:117 XS:i:87 A00126:224:H7HMWDSX3:3:1109:14751:22748 97 Test_seq 171454 60 28M1I122M = 171789 486 GTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTCGATTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTT FFFFFFFFFF,FFF,:FFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FF:FFFFFFFFFFF:FF:FFFFFFFFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,F NM:i:6 MD:Z:71A2T19A34T9T10 MC:Z:151M AS:i:118 XS:i:32 A00126:224:H7HMWDSX3:3:1109:14751:22748 145 Test_seq 171789 60 151M = 171454 -486 GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTG :FFF::FFFFFFFFF:FFFFFF,F,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFFFFFFF:,FFF:FFFFFFFFFFFF NM:i:1 MD:Z:149C1 MC:Z:28M1I122M AS:i:149 XS:i:0 A00126:224:H7HMWDSX3:3:1109:32579:24314 97 Test_seq 132878 60 151M = 133104 360 GACGAGCCTTGAACGTGTGAGGCTCAGTACTGCTGTGTCTTTCACGTGCGCCGCTGTATTTGTGGCCTCTGTCTCCAGGGGAAGACTGTATTCGCAGCCCCTCATTCTCAGGGTACAACATTTTGTCTTCCGAGCAGCTGGATGTGAAGGC FFFFFFFFF:FFFFFFFFFFFFF,FFFFFF:FFFFFFFF,FFFFFF:FFFFFFFFFF,F:FFFFFFF,FFF,F:FFFFFFFF,FFF:FF:,FFFFFFFFFFFFFF:FFFFFFF:,F,,FF:F:FFFF:FFFFFFF:FF:FFF,FFF,FFFF NM:i:0 MD:Z:151 MC:Z:134M17S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1109:32579:24314 145 Test_seq 133104 60 134M17S = 132878 -360 TCAAACACTTATACACCAAGTCCTCACTTATCAACATGTTTAGGTTCCAAAGTCTAGGTTGTTATACAAAAATCGTCATTAAGCAAAAATGGTAGATGACTACAACATTACATAACTGCCAGACCACTAAGAATTAAAGTGACAGCTTGAA FFFFFFFFF:F::F,FF:F,FFFFF:F:,FFF::F::FF,,FFF,FFFF,:FFF:FFFFFFF:FFFFFFF:FFFFFFFF:,FFFF:FFFFFFFFFF,FFFF,F:FFF::FF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFF:F,FF:F:FF NM:i:1 MD:Z:19T114 MC:Z:151M AS:i:129 XS:i:0 A00126:224:H7HMWDSX3:3:1109:17246:27352 97 Test_seq 81985 60 151M = 97440 15476 TTTTTGTTATTGTGAACAATGCAGTGAACATTGGTGTGCATATGTCTGTTTGTGTGACGGCTCTTATTTCTCTAGGATATATTTCTAGGAATGGGATTGCTGGATCATACGGTATTTGTATTTGTAGCTTTTTAAGGAAGCACCATATCAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:130S21M AS:i:151 XS:i:81 A00126:224:H7HMWDSX3:3:1109:17246:27352 145 Test_seq 97440 12 130S21M = 81985 -15476 GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGATCCAGTTCTTTTAGAAGCC FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:0 MD:Z:21 MC:Z:151M AS:i:21 XS:i:0 SA:Z:Test_seq,82184,-,132M19S,60,0; A00126:224:H7HMWDSX3:3:1109:17246:27352 2193 Test_seq 82184 60 132M19H = 81985 -331 GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGA FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:132 MC:Z:151M AS:i:132 XS:i:21 SA:Z:Test_seq,97440,-,130S21M,12,0; A00126:224:H7HMWDSX3:3:1109:17788:27821 81 Test_seq 169070 60 151M = 168737 -484 TTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAGTTTTACATAGTA FFFFFFFF::,FFFFFFFFFFFFFFF:FFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFF NM:i:7 MD:Z:84G2T3G7C31C6A6G5 MC:Z:151M AS:i:116 XS:i:66 A00126:224:H7HMWDSX3:3:1109:17788:27821 161 Test_seq 168737 60 151M = 169070 484 TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGACCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGT FFF,FFFFFFFFFFFF:FFFFFFF:FFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:F,FFFFFFFFFFFF:FFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FF,F,FFF:FFFFF::FFF NM:i:1 MD:Z:38T112 MC:Z:151M AS:i:146 XS:i:81 A00126:224:H7HMWDSX3:3:1109:12346:31078 81 Test_seq 171476 60 19M1D132M = 171229 -399 TCTTCATTTTTTTAAATTCTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTTCTCTGACTTTCTATTGTGTTGTCTAATTCTGTAATTTTATTGTT :FFFFFFFFF:FFFFFF:FFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:13T5^T29A74C27 MC:Z:151M AS:i:129 XS:i:26 A00126:224:H7HMWDSX3:3:1109:12346:31078 161 Test_seq 171229 60 151M = 171476 399 TTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATCATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACT :FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFF::FFF:FFFFFF:F:FFFFFF:FF:FFFFFFFFFFFFF NM:i:1 MD:Z:114G36 MC:Z:19M1D132M AS:i:146 XS:i:27 A00126:224:H7HMWDSX3:3:1109:30852:31782 97 Test_seq 166084 60 151M = 166403 470 TCAGGAGTAGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTGGGTCTCTGGTTCATGTGGCCCTTTCTATCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF,FF,FFF,FFFFFFFFF,:FF,FFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:8T77A26G26G10 MC:Z:151M AS:i:131 XS:i:71 A00126:224:H7HMWDSX3:3:1109:30852:31782 145 Test_seq 166403 60 151M = 166084 -470 CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATC F:FFFFFF:FFFFFFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF,FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:102T48 MC:Z:151M AS:i:146 XS:i:40 A00126:224:H7HMWDSX3:3:1109:25617:34178 81 Test_seq 168503 60 151M = 168188 -466 AGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:133A17 MC:Z:148M3S AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1109:25617:34178 161 Test_seq 168188 11 148M3S = 168503 466 GTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTGT :FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFF:FFFFFFFFFF,FFFFFFFFFFFFFF::FFFF:FFFFFFFFF NM:i:2 MD:Z:83T10T53 MC:Z:151M AS:i:138 XS:i:131 XA:Z:Test_seq,-212700,151M,4; A00126:224:H7HMWDSX3:3:1109:11234:36667 65 Test_seq 151130 13 51M = 214124 62995 AAACAGTGCTGCAATAAACATGGGTGTGCATATATCTGTTCGTGTAAAGGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFF:FFFF NM:i:1 MD:Z:41A9 MC:Z:51M AS:i:46 XS:i:41 XA:Z:Test_seq,+400281,51M,2; A00126:224:H7HMWDSX3:3:1109:11234:36667 129 Test_seq 214124 13 51M = 151130 -62995 GCCTTCACACAAACAGATATATGTACACCCATGTTTATTGCAGCTCTGTTT FFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:23C27 MC:Z:51M AS:i:46 XS:i:41 XA:Z:Test_seq,-166860,51M,2; A00126:224:H7HMWDSX3:3:1109:15076:36965 97 Test_seq 162165 60 151M = 162426 384 TGGGGGGTTGTAAGTCATAAGTGGTCTGACAGAACAAAACAAAATTATTTGCATCAGATTAAAACAAAGAACAAAGTCATTAACGTTAGGTCAAAACAAAGTCGTTAACAGAGGTATGTGAAGGAGGAGGCAGGCAGAGGGAGGAGAAAAG FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFF:FFFF:FFFFFFF:FF:FFFFFFFFFFFFFFFFFF,FFFFFFF,FFFF:F NM:i:1 MD:Z:84C66 MC:Z:123M28S AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1109:15076:36965 145 Test_seq 162426 60 123M28S = 162165 -384 AAAACCCTATGGAAGAGTTCTTCTGTGCACACATGGGGTGGTCACGAGTCAGAATCGACTGGACTAGCAACTAACAACAGCAGGCAAGTTCGAAGGGTCCTCCTGGGTAGTTAGATGGGATCTAATGGTCTCTTGCTTTCTTCATGTCCTC FF,FFF:F:FFFF:FFFFFFFFF:FFFFFFFF:,FFFFFFFFFFFF,FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFF NM:i:0 MD:Z:123 MC:Z:151M AS:i:123 XS:i:0 A00126:224:H7HMWDSX3:3:1110:14624:3364 97 Test_seq 168277 48 17S134M = 168524 398 CTCTGACAAGGTGGACTACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTGTACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTC FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFFFFF:FF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFF,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,,FFFF NM:i:5 MD:Z:60A3A4G18A28T16 MC:Z:151M AS:i:109 XS:i:84 A00126:224:H7HMWDSX3:3:1110:14624:3364 145 Test_seq 168524 60 151M = 168277 -398 CTCCGTTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGAT FFF:FFFF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:4A146 MC:Z:17S134M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1110:14534:3646 97 Test_seq 168277 47 17S134M = 168524 398 CTCTGACAAGGTGGACTACTCTTCCAAACTCTTCTAGTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTGTACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTC FF:F:FFF:FFFFFF,FFF:FFFFFFFFF:::FFFF,FF:F,FFF,FFFFFFFFFFFFFFFFFF:FF:FFFFFFFFF:FFFF:FF,FF:FFFFFF:FFF:FFFFF,FFFFF,:FFFFFF:F,FFFFF:FFF,FF,FFFFF::FF:FFF:FF NM:i:6 MD:Z:19T40A3A4G18A28T16 MC:Z:151M AS:i:104 XS:i:79 A00126:224:H7HMWDSX3:3:1110:14534:3646 145 Test_seq 168524 60 151M = 168277 -398 CTCCGTTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGAT FF:FFFFFFFF:FF:FFFF:,:FFFFF:FFF:FF:FFFFFF:FF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF NM:i:1 MD:Z:4A146 MC:Z:17S134M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1110:14841:4492 81 Test_seq 170172 60 151M = 169863 -460 GTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:1 MD:Z:24A126 MC:Z:151M AS:i:146 XS:i:74 A00126:224:H7HMWDSX3:3:1110:14841:4492 161 Test_seq 169863 60 151M = 170172 460 TCCATGTGCGCTAGAAAAAAAAGTATATTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCTATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTCCTTCTCCGAAAGT FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF NM:i:3 MD:Z:3C5A101G39 MC:Z:151M AS:i:137 XS:i:34 A00126:224:H7HMWDSX3:3:1110:3378:5181 81 Test_seq 167526 52 151M = 167332 -345 TTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGGCTATCA ,FFFFFFFF::FFFF,FFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:144A6 MC:Z:15S136M AS:i:146 XS:i:121 XA:Z:Test_seq,+213362,98M2I23M2I26M,6; A00126:224:H7HMWDSX3:3:1110:3378:5181 161 Test_seq 167332 21 15S136M = 167526 345 TATAGATTACATTTAGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATAATTTTTGGTAA FFFFF:FFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFF:FFFFF:FFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFF:F:FFFFFFFF,FFFF:FFFFFFFFFFFFF,FFFFFFF:FFF NM:i:2 MD:Z:36A87C11 MC:Z:151M AS:i:126 XS:i:116 XA:Z:Test_seq,-213567,136M15S,4; A00126:224:H7HMWDSX3:3:1110:12020:6057 81 Test_seq 171350 60 121M30S = 171156 -315 GGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTATGAGGATGGTGTTTCATTCTCTTGTACTCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:50G15T54 MC:Z:151M AS:i:111 XS:i:0 A00126:224:H7HMWDSX3:3:1110:12020:6057 161 Test_seq 171156 60 151M = 171350 315 CTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:121M30S AS:i:151 XS:i:35 A00126:224:H7HMWDSX3:3:1110:15130:6151 65 Test_seq 333515 0 62M = 383986 50472 CCTTTGCATAGTCAATAAAACACAGGTAAACATCCTTCTGGTATTCTCTGCCTTCAGCCAGG FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFF,F::FFFFFFFFF:FF,F,:,F NM:i:1 MD:Z:51T10 MC:Z:62M AS:i:57 XS:i:57 XA:Z:Test_seq,-383986,62M,1;Test_seq,+15151,62M,2;Test_seq,+316240,19M2I41M,3; A00126:224:H7HMWDSX3:3:1110:15130:6151 129 Test_seq 383986 0 62M = 333515 -50472 CCTGGCTGAAGGCAGAGAATACCAGAAGGATGTTTACCTGTGTTTTATTGACTATGCAAAGG FFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FF:FFFFF,FFFF::FFFFFF,F:FFF:F:FF NM:i:1 MD:Z:10A51 MC:Z:62M AS:i:57 XS:i:57 XA:Z:Test_seq,-333515,62M,1;Test_seq,-15151,62M,2;Test_seq,-316240,19M2I41M,3;Test_seq,+449331,62M,4; A00126:224:H7HMWDSX3:3:1110:18936:8046 81 Test_seq 168571 60 151M = 168392 -330 ATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAA FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF NM:i:1 MD:Z:145T5 MC:Z:151M AS:i:146 XS:i:73 A00126:224:H7HMWDSX3:3:1110:18936:8046 161 Test_seq 168392 60 151M = 168571 330 TCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCATGATGTTGAATAAGAGCGGTGATAAGGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFF NM:i:6 MD:Z:2T52T1C24A20T44A2 MC:Z:151M AS:i:125 XS:i:73 A00126:224:H7HMWDSX3:3:1110:19090:8406 113 Test_seq 169254 60 151M = 52324 -117058 TGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTG FFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFF:FFFFFFF:FFFFFFFF:FFFFF:F:FFFF:FFFFFFFFFF:FF:FFFFFFFFF:FFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFF:FF,F:FF NM:i:1 MD:Z:14T136 MC:Z:127S24M AS:i:146 XS:i:82 A00126:224:H7HMWDSX3:3:1110:19090:8406 177 Test_seq 52324 0 127S24M = 169254 117058 AGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGATGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAACTCTCTGTCTCTTTATTGGTGCA FFFFFFFFF:F,,FFFFFF:FF::F,F,FFFF:FFFF:F:FFFFFFF,FFFF:FF,F:FFF,FFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:, NM:i:0 MD:Z:24 MC:Z:151M AS:i:24 XS:i:24 SA:Z:Test_seq,168994,+,22S129M,60,1; XA:Z:Test_seq,-154647,127S24M,0;Test_seq,+255573,24M127S,0;Test_seq,+38332,24M127S,0;Test_seq,-170380,127S24M,0; A00126:224:H7HMWDSX3:3:1110:19090:8406 2209 Test_seq 168994 60 22H129M = 169254 411 GTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCT FFFFFFFFFFFFFFFFFFF,FFFFF:FF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFF,FFF:F,FF:FFFF,FFFFFFF:F:FFFF:FFFF,F,F::FF:FFFFFF,,F:FFFFFFFFF NM:i:1 MD:Z:44G84 MC:Z:151M AS:i:124 XS:i:49 SA:Z:Test_seq,52324,-,127S24M,0,0; A00126:224:H7HMWDSX3:3:1110:18765:8531 97 Test_seq 169027 60 151M = 169352 476 GCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFF,FFF:FFFFFFFFFF:FFF:FFFFFF::FFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF,F:FF:FF,FFFFF:FF,:F NM:i:5 MD:Z:14T23A57A37G1A14 MC:Z:151M AS:i:126 XS:i:61 A00126:224:H7HMWDSX3:3:1110:18765:8531 145 Test_seq 169352 60 151M = 169027 -476 TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTG FFFFFFFFF:FFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:115G35 MC:Z:151M AS:i:146 XS:i:101 A00126:224:H7HMWDSX3:3:1110:23927:10676 81 Test_seq 166345 60 151M = 166048 -448 TTTTCATAATAGAATTATTTTGCCCATTGACTTAGAAGTCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAGGCATTCATTTTATCCTGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTC FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:6 MD:Z:25G14T10T38A2T12C44 MC:Z:151M AS:i:121 XS:i:0 A00126:224:H7HMWDSX3:3:1110:23927:10676 161 Test_seq 166048 60 151M = 166345 448 AATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCTTTTCTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:143C7 MC:Z:151M AS:i:146 XS:i:45 A00126:224:H7HMWDSX3:3:1110:29161:13416 97 Test_seq 169352 60 151M = 169628 427 TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTG FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFF:FFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,FFFFFFFFFFFF,FFFF: NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:66 A00126:224:H7HMWDSX3:3:1110:29161:13416 145 Test_seq 169628 60 151M = 169352 -427 CGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATGTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCCTTGTGGTCTGA FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:0T34G54T28C31 MC:Z:151M AS:i:135 XS:i:62 A00126:224:H7HMWDSX3:3:1110:18873:14356 81 Test_seq 168252 41 92M4I55M = 167906 -493 TTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTGGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCGATCATGTCATCTGCAAATAGTAATAGCTTTACTTCTTCCTTGCCAATCTGGAT F:FFF:F,FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:11 MD:Z:19T10T15A47G18A1G13C17 MC:Z:151M AS:i:102 XS:i:77 A00126:224:H7HMWDSX3:3:1110:18873:14356 161 Test_seq 167906 60 151M = 168252 493 TTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTAAATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFF,FFFFFFF:FF,FFFFF,FFFF,FFFF:FFFFFFF:FFFF:FF,F:,,FFF,F:F,:FFFFF:::FFFF: NM:i:1 MD:Z:80T70 MC:Z:92M4I55M AS:i:146 XS:i:76 A00126:224:H7HMWDSX3:3:1110:29297:15468 97 Test_seq 168209 26 114M4S = 168209 118 CTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTAAGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,F NM:i:1 MD:Z:62T51 MC:Z:118M AS:i:109 XS:i:98 XA:Z:Test_seq,-212716,4S114M,4; A00126:224:H7HMWDSX3:3:1110:29297:15468 145 Test_seq 168209 28 118M = 168209 -118 CTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTT FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:62T55 MC:Z:114M4S AS:i:113 XS:i:101 XA:Z:Test_seq,+212712,118M,5; A00126:224:H7HMWDSX3:3:1110:9796:16736 81 Test_seq 167331 23 151M = 167173 -309 TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:37A113 MC:Z:16S135M AS:i:146 XS:i:135 XA:Z:Test_seq,+213553,151M,4; A00126:224:H7HMWDSX3:3:1110:9796:16736 161 Test_seq 167173 27 16S135M = 167331 309 AGATGCTAGATGGATCGATCGAGAGCATTTTCTCATGTACCTGTCAGCTGCCTGAATATCTTCCTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF, NM:i:5 MD:Z:23T4T0G17T22T64 MC:Z:151M AS:i:110 XS:i:96 XA:Z:Test_seq,-213727,135M16S,8; A00126:224:H7HMWDSX3:3:1110:30951:17675 81 Test_seq 449108 27 42M10S = 383784 -65366 CTGACTGGAAGAGATCCATATTTATGCCTATTCCCAAGAAAGCCGATGCAAC FFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFF NM:i:0 MD:Z:42 MC:Z:52M AS:i:42 XS:i:37 XA:Z:Test_seq,+15394,10S42M,1;Test_seq,+47309,10S39M3S,1; A00126:224:H7HMWDSX3:3:1110:30951:17675 161 Test_seq 383784 0 52M = 449108 65366 CTGACTGGAAGAGATCCATATTTATGCCTATTCCCAAGAAAGGTGATCCAAC FFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FF:FFFFF::FF NM:i:1 MD:Z:20C31 MC:Z:42M10S AS:i:47 XS:i:47 XA:Z:Test_seq,-333748,52M,1;Test_seq,-15384,52M,1;Test_seq,+449108,52M,2; A00126:224:H7HMWDSX3:3:1110:13666:17832 81 Test_seq 168630 60 42M4I105M = 168483 -294 GTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACAGTAATGGTTTTCCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATC FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:9 MD:Z:6A26T52T1T10T47 MC:Z:151M AS:i:112 XS:i:58 A00126:224:H7HMWDSX3:3:1110:13666:17832 161 Test_seq 168483 60 151M = 168630 294 TTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:12T138 MC:Z:42M4I105M AS:i:146 XS:i:93 A00126:224:H7HMWDSX3:3:1110:2636:18176 97 Test_seq 167381 0 73M = 167381 73 CTCTTTTGGAGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTC FF:FFF:,FFF:F,FF,:F:F:F,::F:FF:::,FFFFFFFFFFF,:F,FF,FFFFF:FF:FFFFFFFFFFF: NM:i:1 MD:Z:9T63 MC:Z:73M AS:i:68 XS:i:68 XA:Z:Test_seq,-213581,73M,1; A00126:224:H7HMWDSX3:3:1110:2636:18176 145 Test_seq 167381 0 73M = 167381 -73 CTCTTTTGGAGAAGTATTTAGTTGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTC FF,FFFF:F,FFFFF,FFF:,,FFF,F:FFFFFF,FFFFFFFFFF:FFFFF,:FFFFFFF,FF:FFFFFFFF, NM:i:3 MD:Z:9T5C5A51 MC:Z:73M AS:i:58 XS:i:58 XA:Z:Test_seq,+213581,73M,3; A00126:224:H7HMWDSX3:3:1110:12156:19695 81 Test_seq 170207 60 151M = 170074 -284 TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATC :FFFFF:FFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F NM:i:1 MD:Z:55A95 MC:Z:151M AS:i:146 XS:i:88 A00126:224:H7HMWDSX3:3:1110:12156:19695 161 Test_seq 170074 60 151M = 170207 284 AAAATTTGATTTATGTATCTTGCAGCCCTGTCATTAGGTGCGTAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFF,::FFFFFFFFFFFFF,F,F, NM:i:5 MD:Z:33C1G26A58C0A28 MC:Z:151M AS:i:126 XS:i:82 A00126:224:H7HMWDSX3:3:1110:1859:20588 97 Test_seq 228268 60 151M = 228489 372 GTTTACTCAAATGGACACTCATTCCTCTAGTAATTTTTTCAAGTATGCCTCATTGCTTTTCTATAAAACAAGCGCTGAAGAACTCTCAGAGGGCTGGTGCAAGCTTTGAGCATATTAGCTCTTTATTAGAGGTCTTGAATGCCCACACAGC FFFFFFFFF:FFF:FFFFFFFFF,FFFF::FFF,FFFFFFFFFFFF:,FF:FFFFFFFFF:FFFFFF:FFF,F,FFFFFFFFFFF:FFFF:,:FF,FFFFFFFFFFFFFF:FFFFFF,:FFFFFFFFF,F,FFFFF,FFFF,:,,F:,FFF NM:i:2 MD:Z:33G110C6 MC:Z:151M AS:i:141 XS:i:0 A00126:224:H7HMWDSX3:3:1110:1859:20588 145 Test_seq 228489 60 151M = 228268 -372 AACCCCGATAACTGCTGTTTGAGAAGTCACTCTGGAAGAATTAAAAACAAAAACCCGTTGCTGCTGAGTTGATTCTGACTCGTAGCGACCCTACAGGACACAGTAGAAATGCCCCTATAAGGCTTCTAAGGAGTGGCTGGTGGATTCAAAT FFF:,F,FF:F::F:F,FFF:FF,,:,FFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,FFF:FFFFFFFFFF:F:F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFFFF NM:i:1 MD:Z:26G124 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1110:31114:23375 81 Test_seq 168525 60 151M = 168238 -438 TCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC FFFFFF,FFFFFFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:1 MD:Z:69A81 MC:Z:151M AS:i:146 XS:i:87 A00126:224:H7HMWDSX3:3:1110:31114:23375 161 Test_seq 168238 56 151M = 168525 438 AATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACACTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::FFFFFFFFFFFFF NM:i:6 MD:Z:33T7T65G0G18A21G1 MC:Z:151M AS:i:124 XS:i:94 A00126:224:H7HMWDSX3:3:1110:14660:24721 97 Test_seq 171186 60 151M = 171417 379 AGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTCTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTC FFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF NM:i:1 MD:Z:94T56 MC:Z:3S65M1D22M1I60M AS:i:146 XS:i:67 A00126:224:H7HMWDSX3:3:1110:14660:24721 145 Test_seq 171417 60 3S65M1D22M1I60M = 171186 -379 CCGGGACTCCAGTCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTAAATTCTTTTATCTGATTTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTC FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFF NM:i:5 MD:Z:8A56^T6T35A39 MC:Z:151M AS:i:118 XS:i:39 A00126:224:H7HMWDSX3:3:1110:30897:25097 81 Test_seq 186506 60 151M = 186269 -388 CTTATATTTAAAAAAAAAAAAAAGAGAACCTTAGTTTTCAAAAGACACAAGACTTAGATTAGAAGTGCCTACCACATGTCTAGCATGGTGAATTAAAAACAACAACAACAACAAAAAAGGTAACTTGTGAAACTTTAGAACACCAAAGATT ,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:22S129M AS:i:151 XS:i:23 A00126:224:H7HMWDSX3:3:1110:30897:25097 161 Test_seq 186269 60 22S129M = 186506 388 ATTTATTTATAGTTCTGTGAGTGTCTCTAAAGGGTTGTGGACATTGGGGTTGCAGACTGAGCTAGTCAGTGACAGCCCACTCAACTTTTGCTCATGCAGCCCTTCTGATGCTTATTCCTTGTTTTAATAAAAAGACTTCCTACCAGAAACA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFF NM:i:0 MD:Z:129 MC:Z:151M AS:i:129 XS:i:0 A00126:224:H7HMWDSX3:3:1110:2166:26412 97 Test_seq 156809 60 151M = 157092 434 GGAGCCTGCGAACAAGGGCCTATTCTCCCGAAATAGGCCCACATAGGTCCATGCAGAAGGGAAAGGTGCTCAAAGTCCACGGACAGTTTATGCCTGGATAGGAGCTGCTTCTGTCCTGAGCTCCCCTGGTTAGTGGAACTAGCAAATTATC FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:F:FF::,FFFF,FFFFFFFFFFFFFFF:FFFFFFFF:FFFF:F:FF:FFFFFF:,:FFFFFFFFF:FF:FFFFFFFFFFFFF:FFF,FFFFFFFFF:FF:FF:FFFFFFFFF:F NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:97 A00126:224:H7HMWDSX3:3:1110:2166:26412 145 Test_seq 157092 60 151M = 156809 -434 TATGCAAGTACTTAGCTTTTGCCGAGAGCACTGTCGTTCTCAGGTTCCGGAGGTGTGAGTAGACTGTGTGGCTGGCTGCTTCTCCCTGAGGAAACTGTAGCCAAACGCTAGTACCAGCCCACCACCGCCGCTGCCGTGGGAATGGTGCCTG FFFFF:FFFFFFFFFFFFFFFF:FF,FFF:F:F:FFFFFFFFFFF:FFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:55 A00126:224:H7HMWDSX3:3:1110:19162:27007 97 Test_seq 171120 60 151M = 171331 336 ATTCTCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,,,,F:FFFFFFFFFFFFFFFFFF,:FFFFF,FFFF:FFFFFFF,FFFFFFFFFFFF:FFFFFF::FF:FFFFFFF,FFFF NM:i:1 MD:Z:4C146 MC:Z:125M26S AS:i:146 XS:i:70 A00126:224:H7HMWDSX3:3:1110:19162:27007 145 Test_seq 171331 60 125M26S = 171120 -336 TTCATCTTTCATGATGTCAGGGAAGTTTTGTGTAAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAGTCACTATTAGGGTCACTAGGGGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:3G29C35G15T39 MC:Z:151M AS:i:106 XS:i:40 A00126:224:H7HMWDSX3:3:1110:19407:28902 97 Test_seq 167163 52 151M = 167410 373 AATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTA FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFF:FFFFF::FFFFFFFFFFFFFFFFFFF::FF,FFF::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:80T70 MC:Z:126M25S AS:i:146 XS:i:121 XA:Z:Test_seq,-213721,151M,6; A00126:224:H7HMWDSX3:3:1110:19407:28902 145 Test_seq 167410 19 126M25S = 167163 -373 AGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCGATCATTCGTCTTTTTATTGTTGAG FFF:FFF:FFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:6 MD:Z:32T44G2C22A0T2T18 MC:Z:151M AS:i:96 XS:i:86 XA:Z:Test_seq,+213499,25S126M,8; A00126:224:H7HMWDSX3:3:1110:19180:29136 97 Test_seq 32280 60 20S131M = 32345 216 GGAGAAGTTCATTTTAAGAGTAAAATGCCTTTCATTTTTCAGTGCAATTAGAAAATCCTCTTGGAAGCTCAAAGTTTATACAGAGTTTTTTAAAATATACTCTTGATTGTATTTTAGCATTCTTTATTTATTTATTTTGTATTTTATATAA FFFFFFFFFFF,:FF,F,F:FFFFFF,:F,F:,F,FFFF,F,:FFFF,F:,FFFF,,,,,:F:F:F,F,,FFF,:FF,FFF,,::F:FFFF,FFF,:,,:,,:F,:FFFFFF,F,:,:FFF,:FFF:FFFFFF:FFFF,:,FFFF,,FFFF NM:i:7 MD:Z:57C3T14C1C13G1G31G4 MC:Z:151M AS:i:96 XS:i:0 A00126:224:H7HMWDSX3:3:1110:19180:29136 145 Test_seq 32345 60 151M = 32280 -216 TTTTTTAAAATCTCCTCTTGATTGTATGTGAGCATTCTTTATTTATTTATTTTGTATTTTAGATAAAGGTTTACAGAGGAAATTAGTATGACAGGATCTAAAAGTGCTTAATGTTCTTGAAGTAACATATTTAATGGGTGTCTGCTTCTCA FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:20S131M AS:i:151 XS:i:23 A00126:224:H7HMWDSX3:3:1110:10628:29105 97 Test_seq 212528 7 151M = 212713 336 ATTTCCCTTGAGAACGGGAACCAGACAAGGATGCTCTTTAACACCGCTCTTATTCAACATTATGCTAGAAGTCCTAGCCAGAGCAATTAGGCTAGACAAAGAAATAAAGGTCATCCGGATTGGCAAAGAGGAAGTAAAATTATCTCTATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:14 MD:Z:0C4G4G8G5A8C5T3T11G4G7G40G4T10G24 MC:Z:151M AS:i:85 XS:i:78 XA:Z:Test_seq,-168373,13S138M,12;Test_seq,+40206,39M3I109M,16; A00126:224:H7HMWDSX3:3:1110:10628:29105 145 Test_seq 212713 31 151M = 212528 -336 AGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGC FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:113A37 MC:Z:151M AS:i:146 XS:i:131 XA:Z:Test_seq,+168175,151M,4; A00126:224:H7HMWDSX3:3:1110:18475:29481 81 Test_seq 166403 60 151M = 166060 -494 CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCAGAAACCTCTTTGCTTTTAATCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACAGATTGATC FFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:49G18G33T39T8 MC:Z:151M AS:i:131 XS:i:40 A00126:224:H7HMWDSX3:3:1110:18475:29481 161 Test_seq 166060 60 151M = 166403 494 GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCAATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCT FFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FF:FFFF:,FF NM:i:1 MD:Z:64G86 MC:Z:151M AS:i:146 XS:i:85 A00126:224:H7HMWDSX3:3:1110:23366:30655 97 Test_seq 168163 0 151M = 168481 469 ATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACTTGAGACTCTGCCAAACTGTTCTATTAGTTTCAGTAGTTTTC FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FF:FFFFFFF NM:i:5 MD:Z:6C101T0C9T7C23 MC:Z:151M AS:i:126 XS:i:126 XA:Z:Test_seq,-212725,151M,5; A00126:224:H7HMWDSX3:3:1110:23366:30655 145 Test_seq 168481 60 151M = 168163 -469 CCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGT :::FF:FFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:1 MD:Z:14T136 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1110:1678:32330 97 Test_seq 163369 60 151M = 163597 379 AGCCAGAAGCATAGCATCTACAGTCTTTCTGACTCTGTGTCCATTAATCACAGCTGCTTCTCTAACTCCGACCTCTGATAAGGACCCTTGGGATAATCTCTTCATCTCAAAATCCTTATTTCAAACACATCTGCAAAGCCCCTTTTGCCAT FFFFFFFFFFF,:FFFFFFF:FFFFF:F:FFFFFFFFFFF,,:FFFFFFFFFFFFFFFFFFFFFF:F::FF,FFFF:F:FF:F,F,FF:F,FFFF,F:F:F:FFFFF::FFFF:FFF:FF:FFF,FFFF:,F,:FFFF:FFFFF,F::FFF NM:i:1 MD:Z:124C26 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1110:1678:32330 145 Test_seq 163597 60 151M = 163369 -379 AGTGTATCATAGAAAATACATTGATGCCAGCACTGGTTTTAGAGCAGACCTGAGTTCAAATCCTGCCTTCAGCACCTAGTAGTTGTGTGACCATCGTGCCTTTGGGTTTCTGTTTTTTCATCCATAAAATAAGACTAAAATCCCAAGATTA FF:,FF,:FFFFFFFFFFFFF:FF:::FFF:FFFFF,FFFF,FF:FFFF::F:,::,FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF,FFFFFFF,F::FFFFFFFF:FF:FFFFFF:FFF:FF:FFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:40 A00126:224:H7HMWDSX3:3:1110:23809:32706 97 Test_seq 150778 60 151M = 151034 407 GTTGTCTTTCCCTTCACCTAAACTAGTTCTTAACTACTATCTAATTAGTGAATAGCCCCGCCCACGGTCCCGTCCCTCCCCCACTGGTAACCCCAGAAGAAAGTTTTCTTCTCAGTTTAAAATAATTATAAAGTTCTTATAATAGTGGTCT FFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFF::::,:,FF,:FFFF,,,,,,,,FFF,:FFFF,F,FF,FFFF,F:,:F,,F,,:,,::,:,,,F,,,FFF::,F,,F::,:F,:F,F,::F:FF,:,:::F NM:i:11 MD:Z:54C4T6T4T10T9A8T19C2T2C1C21 MC:Z:151M AS:i:96 XS:i:30 A00126:224:H7HMWDSX3:3:1110:23809:32706 145 Test_seq 151034 60 151M = 150778 -407 CGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTGTTCATGTAAAGGCTCCT FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:69 A00126:224:H7HMWDSX3:3:1110:15799:33113 97 Test_seq 173420 60 151M = 173609 340 ATTCTGCCCAGCTCTGCCTTACTCTCAGTCCCACTGCTTTTCCCATAAGGCCATGGAATCAGGCATGATGTGTAGAAGCATAAACTGGGGGGTCACTGAGTGAAGCAATTCTGACTAACACAGTCAGAGGTTCTCCACAGCCTTGAACGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF,FFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFF,FFFF:FFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1110:15799:33113 145 Test_seq 173609 60 151M = 173420 -340 CCAGCGTTGGATTAGACTGGACCTTCTTTGAGGAGATGTCAAGGAAGGACTGTTCAGGAGCCAAGGGAAGTGTTCTTCATGGTGGCAATAACTGAAGTCTCCACTTAGGTCCTCGATATTTCCTAAGCTTGTTGTCATTAGTTGCTCTAAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1110:3812:33990 81 Test_seq 169359 60 151M = 169218 -292 AAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTCGGGAC ,FFFFFFFF,FFFFFFF,FFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:146A4 MC:Z:151M AS:i:146 XS:i:66 A00126:224:H7HMWDSX3:3:1110:3812:33990 161 Test_seq 169218 60 151M = 169359 292 GTAATCTGAAATGATTCTTTTAATTTCATTTGGTTCTGTTGTGATGTGGTCCTTCTCGTTTCTTATTTGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAG F:FFFFF:FF:F:F:FFF:F:FF::F:FF:FF:F,FFF:FFFFF:FFFF:F:FFFFFF:FFF:,FF,FFFFFFFF,FFFFF,F,FFFF:FFFFF:FFF:F:F:FFFFFFF,FFF:F:FFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF NM:i:4 MD:Z:33C16T16C23T59 MC:Z:151M AS:i:131 XS:i:72 A00126:224:H7HMWDSX3:3:1110:9489:34428 81 Test_seq 170319 60 151M = 170166 -304 TCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:1 MD:Z:57C93 MC:Z:151M AS:i:146 XS:i:64 A00126:224:H7HMWDSX3:3:1110:9489:34428 161 Test_seq 170166 60 151M = 170319 304 TATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTTTTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTA FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFFF,FFFFFF:FFFFFFFFFFF:FFFF NM:i:4 MD:Z:29C0A18A46A54 MC:Z:151M AS:i:131 XS:i:75 A00126:224:H7HMWDSX3:3:1111:24768:1360 97 Test_seq 168456 60 151M = 168549 220 GAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:39T111 MC:Z:127M24S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1111:24768:1360 145 Test_seq 168549 59 127M24S = 168456 -220 TGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTGTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCCTCTCCTTTCTCTGTCGCTCTAGT FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:60A7T18A26T12 MC:Z:151M AS:i:107 XS:i:78 A00126:224:H7HMWDSX3:3:1111:29586:1720 97 Test_seq 168277 60 26S125M = 168524 398 AACATAGTAATATTTTACATATTTTAACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTCTACTTCCTCCTTGCCAATCTGGATACC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:3 MD:Z:69G18A8T27 MC:Z:151M AS:i:110 XS:i:72 A00126:224:H7HMWDSX3:3:1111:29586:1720 145 Test_seq 168524 60 151M = 168277 -398 CTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGAT FF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:26S125M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:19352:2284 65 Test_seq 213219 1 151M = 167332 -45888 GCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGCTGATATTTGACAAAGGACCAGTGTCGGTCAATTGGGGAAATGATAGTC FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:F:FFFFFFFF:FFF:FFFFFFFFF NM:i:2 MD:Z:93A34A22 MC:Z:151M AS:i:141 XS:i:139 XA:Z:Test_seq,-167669,151M,3; A00126:224:H7HMWDSX3:3:1111:19352:2284 129 Test_seq 167332 21 151M = 213219 45888 GAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGT FFFFFFFFFFFFFFFFFFFFFF:FF,,:FFFFFFFFF,:FFFFFF:FFFFFFF:FFF:FFFF,F,FFFF,FFFFF,FFFF,F:FF:F,F:FFF:F:FF:FFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFF:FFFF,:FFFFFFFFFF NM:i:1 MD:Z:36A114 MC:Z:151M AS:i:146 XS:i:136 XA:Z:Test_seq,-213552,151M,3; A00126:224:H7HMWDSX3:3:1111:26467:7968 81 Test_seq 168683 60 128M23S = 168497 -314 TTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGTATTATTTTTTTGATGTGTTGTTGGATTAGTCCATCTTAGAACATGGAAGA FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:33T66A27 MC:Z:151M AS:i:118 XS:i:55 A00126:224:H7HMWDSX3:3:1111:26467:7968 161 Test_seq 168497 60 151M = 168683 314 TTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,:F:FFF:FFFFFFFFFFFFFFF:FFFFFFFFF:,FFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:128M23S AS:i:151 XS:i:96 A00126:224:H7HMWDSX3:3:1111:29125:8124 97 Test_seq 391538 60 151M = 391803 391 GGTGCTCCTTGGAAACCCTGTGGGGCAGTTCTACTCTGTCCCATAGGGTCTCTATGAGTCGGAATTGTCTCAAGGGCATCGGGTATGTATGTATATATGTAGAAATGACAGATTTTGCCATGTTAGTTTTCCACAATGTTTTCGGTGAAGT FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:126M25S AS:i:151 XS:i:31 A00126:224:H7HMWDSX3:3:1111:29125:8124 145 Test_seq 391803 60 126M25S = 391538 -391 AGCTGTACACTTGAGAGATAAACAGTTAAAAAGTGCCTTATGTGCCTTCCTGGTGTTTTCTGTGCACATATGGCCATATATTATGGGCATACCAAATCAAAAAAACAAGTTGCTGTCCAGGTGATCGATCCCTGTGCTGACTTTGGAATTC :FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:126 MC:Z:151M AS:i:126 XS:i:0 A00126:224:H7HMWDSX3:3:1111:2871:8187 81 Test_seq 168516 60 151M = 168350 -317 CAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCAATT FF::F:FFFFFFFFFFFFFFF:FFFFF:FFFFFFF,FFF:,:FFFFFF:,FF,FFFFFFF,FF:F:,FF,FFFFFFFFFFFFFFFF::FF,,FFF:FF,,FFFFF:F:FFFF:FFF,,FFFFF:FF:FFFFFFFFFFFFF,FFFF,FFFFF NM:i:1 MD:Z:147T3 MC:Z:27S124M AS:i:147 XS:i:67 A00126:224:H7HMWDSX3:3:1111:2871:8187 161 Test_seq 168350 38 27S124M = 168516 317 TGTGAGTTCTACCAAACATTTAGATCTTGTCATCTGCAAATAGTGATAACTTTACTTCCTCCTTGCCAATCCAGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCAGTGATAA F:F,F:FFFF:FFFF:FFFFFFF:FFFFFFFFFFFFFFF:FF,FFFFFF:FFFF:F::F:,FFF:,,FFF:F:FFFFF:FFFFFFFF,FFFF:FFF,::FFFFFF,,:FF,FFF:FFFFF,F,,FFFFFFFFFFFFFFFFF:FFFFFFFFF NM:i:5 MD:Z:15A5G22T0G70G7 MC:Z:151M AS:i:99 XS:i:80 XA:Z:Test_seq,-212565,130M21S,10; A00126:224:H7HMWDSX3:3:1111:25301:8672 81 Test_seq 170430 60 120M31S = 170261 -289 TATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGAGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCCGAGACGTTTTTCTTAGTAGATTGTGAGATCAGTCCTACCATTTTACAGTCTTGCCAGCAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:3 MD:Z:49T10T28T30 MC:Z:151M AS:i:105 XS:i:22 A00126:224:H7HMWDSX3:3:1111:25301:8672 161 Test_seq 170261 60 151M = 170430 289 TATTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGGTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF, NM:i:1 MD:Z:44T106 MC:Z:120M31S AS:i:146 XS:i:70 A00126:224:H7HMWDSX3:3:1111:11153:8719 97 Test_seq 170147 60 151M = 170256 260 CAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAGTATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTAATATATTTTTTTACATCCTTTGAGTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F::FFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFF,FF:F:FFF:FFFFF NM:i:4 MD:Z:49A34A38G12C14 MC:Z:151M AS:i:131 XS:i:55 A00126:224:H7HMWDSX3:3:1111:11153:8719 145 Test_seq 170256 60 151M = 170147 -260 CTTATTATTTGCTTAATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:14G136 MC:Z:151M AS:i:146 XS:i:73 A00126:224:H7HMWDSX3:3:1111:21468:9079 81 Test_seq 117697 60 151M = 117403 -445 TCCTCTTTCTGTGGGATGGACAAGGATGGAGCCTAGGGCCCCATCCCTGTCACATTTCTGCACAGTCCTCAGACACTTGCAGAAAGTGTTGCCGGTTTTTAGCACATCTTTCTCTGGAGATGAAACTGGCTCAGCACCAACCAGAGGGCAC FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:21468:9079 161 Test_seq 117403 60 151M = 117697 445 AATCCACTTAAGTATTTGAATGTTATTTCAACCTCTTTCTATGAGGAAGAGCTTTGGGAAAAAATAAGTAGAAAGTGAAAATATCGATAGTGATTAATGAGTGATGGCTGGCTTTGACTGCTGTAGGAGTAGGTAGGACAACCGCTACAAA FFFFFFFFFFFFFF,F:FFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF: NM:i:1 MD:Z:148G2 MC:Z:151M AS:i:148 XS:i:0 A00126:224:H7HMWDSX3:3:1111:3884:9909 97 Test_seq 196478 60 32M119S = 319087 122760 TCCTCATTCATAAATAGGGCTTGTTATAGATCACTCACGGCAACCCCGTGTGTGCAGAGTAGAGCTGCACTCCACAGTGGTTTTCAAGGCTGTGAACTTTCAGAAACAGGTCACTAGTCCTTTCTTTCAAGGTGCCTCTGAGTGGGTTCAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFF NM:i:0 MD:Z:32 MC:Z:151M AS:i:32 XS:i:0 SA:Z:Test_seq,318867,+,32S119M,60,0; A00126:224:H7HMWDSX3:3:1111:3884:9909 2145 Test_seq 318867 60 32H119M = 319087 371 ACTCACGGCAACCCCGTGTGTGCAGAGTAGAGCTGCACTCCACAGTGGTTTTCAAGGCTGTGAACTTTCAGAAACAGGTCACTAGTCCTTTCTTTCAAGGTGCCTCTGAGTGGGTTCAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFF NM:i:0 MD:Z:119 MC:Z:151M AS:i:119 XS:i:0 SA:Z:Test_seq,196478,+,32M119S,60,0; A00126:224:H7HMWDSX3:3:1111:3884:9909 145 Test_seq 319087 60 151M = 196478 -122760 GGTGATGGTTTAGTGGGGTGGATGGGTAGAGTAGTGAGAGGCATCAGCTGCTCTCCTGTTGAAGCTGGAAGACATCATCAAGATCAGACAGCCTGAGAGAAGCGTCTTGGGAGGAAGTGGGTACGAGCATTGGCTGTCTAAGAGTTGAGAT FFFFFFFFFFFFFFFFFF,F:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:32M119S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:27254:10113 97 Test_seq 169321 60 151M = 169628 426 AGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCT FFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,:FFFF:F,FFFF,:FF:FFFF:FFFFFFFFFF:FFFFFFFFFFFF NM:i:1 MD:Z:146G4 MC:Z:119M32S AS:i:146 XS:i:112 A00126:224:H7HMWDSX3:3:1111:27254:10113 145 Test_seq 169628 60 119M32S = 169321 -426 CGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCTCTTTATTTGCACTGCTTTAGG FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFF NM:i:4 MD:Z:0T11A22G70A12 MC:Z:151M AS:i:103 XS:i:0 A00126:224:H7HMWDSX3:3:1111:22200:10222 81 Test_seq 117697 60 151M = 117403 -445 TCCTCTTTCTGTGGGATGGACAAGGATGGAGCCTAGGGCCCCATCCCTGTCACATTTCTGCACAGTCCTCAGACACTTGCAGAAAGTGTTGCCGGTTTTTAGCACATCTTTCTCTGGAGATGAAACTGGCTCAGCACCAACCAGAGGGCAC FFFFFF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:22200:10222 161 Test_seq 117403 60 151M = 117697 445 AATCCACTTAAGTATTTGAATGTTATTTCAACCTCTTTCTATGAGGAAGAGCTTTGGGAAAAAATAAGTAGAAAGTGAAAATATCGATAGTGATTAATGAGTGATGGCTGGCTTTGACTGCTGTAGGAGTAGGTAGGACAACCGCTACAAA FFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFF,FF:FFFF,FFFFFFFFFFFFFFF,F:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFF,FF:FFFFFFFF:F:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:148G2 MC:Z:151M AS:i:148 XS:i:0 A00126:224:H7HMWDSX3:3:1111:17499:11537 97 Test_seq 171298 60 151M = 171509 362 TGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFF:F:FFFFFF,FF:F,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFF,F:FFFFFFFFFF: NM:i:3 MD:Z:62G39G15T32 MC:Z:151M AS:i:136 XS:i:61 A00126:224:H7HMWDSX3:3:1111:17499:11537 145 Test_seq 171509 60 151M = 171298 -362 TTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:74T76 MC:Z:151M AS:i:146 XS:i:47 A00126:224:H7HMWDSX3:3:1111:19994:11788 97 Test_seq 168429 60 151M = 20263 -148141 CCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFF:FF,FFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF, NM:i:1 MD:Z:66T84 MC:Z:124S27M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1111:19994:11788 145 Test_seq 20263 7 124S27M = 168429 148141 TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCTTTTCTTTCTTTTTAATGACCTCTTGC F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:1 MD:Z:6G20 MC:Z:151M AS:i:22 XS:i:20 SA:Z:Test_seq,168657,-,120M31S,60,3; XA:Z:Test_seq,-55966,131S20M,0; A00126:224:H7HMWDSX3:3:1111:19994:11788 2193 Test_seq 168657 60 120M31H = 168429 -348 TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATC F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF NM:i:3 MD:Z:18C30T9T60 MC:Z:151M AS:i:105 XS:i:50 SA:Z:Test_seq,20263,-,124S27M,7,1; A00126:224:H7HMWDSX3:3:1111:20690:11929 97 Test_seq 167298 21 24S50M1I76M = 167447 300 TTTGAAGGCTTGTCTTGTGAATTTAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGACATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTT FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFF NM:i:3 MD:Z:25A44A55 MC:Z:151M AS:i:109 XS:i:99 XA:Z:Test_seq,-213611,74M1I52M24S,5;Test_seq,+400719,24S50M1I76M,7; A00126:224:H7HMWDSX3:3:1111:20690:11929 145 Test_seq 167447 60 151M = 167298 -300 TCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGG FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:24S50M1I76M AS:i:151 XS:i:101 A00126:224:H7HMWDSX3:3:1111:24795:12649 113 Test_seq 168510 60 151M = 212649 44140 TGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:126A24 MC:Z:151M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1111:24795:12649 177 Test_seq 212649 25 151M = 168510 -44140 GGCAAGGAAGAAGTAAAGTTATCACCATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCAACAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF NM:i:10 MD:Z:8G8A5T1T18C0T17T8T0C51G25 MC:Z:151M AS:i:101 XS:i:86 XA:Z:Test_seq,+168239,151M,13; A00126:224:H7HMWDSX3:3:1111:8802:14732 81 Test_seq 170511 60 151M = 170230 -432 TTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTTGTTACGTTTTTCTTGAGTTATGGAATCGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAA FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:76C26T47 MC:Z:151M AS:i:141 XS:i:37 A00126:224:H7HMWDSX3:3:1111:8802:14732 161 Test_seq 170230 60 151M = 170511 432 AATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATCTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGA FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF NM:i:1 MD:Z:47T103 MC:Z:151M AS:i:146 XS:i:72 A00126:224:H7HMWDSX3:3:1111:18756:15843 97 Test_seq 226350 60 151M = 226644 417 GTTTCTTGAATACGTTTTGATATTGTCTGCTGTCCTTGGAACATTCAGTAGTTATTTTCTTCATTTATTGGCTGTAGATTTGTTTGTTTCATCCTGCTTTTTTGTTTTATTTGGTTATGTCTGAGCAGGCAGGCTGTGTGTTCTTTGTTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,:FFF:FF:FFFFFFFFFFFFF:FFFFFFF:FFF,FF:F NM:i:1 MD:Z:12T138 MC:Z:123M28S AS:i:146 XS:i:49 A00126:224:H7HMWDSX3:3:1111:18756:15843 145 Test_seq 226644 60 123M28S = 226350 -417 TGGGCCAGAGGCCAGTGCAGGACAGGCTCTGGTAGGCTATGCCTGTGTTGCTCGAAGGTGCAATGCTCAGCATATGGTGCAGGTAGGCAGGAAGGAGGGGGGAGGTTGTGATGTGTAGAGCTATAAGCACTTTCCATTGTTGTTGTTGTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:123 MC:Z:151M AS:i:123 XS:i:0 A00126:224:H7HMWDSX3:3:1111:23647:16172 97 Test_seq 165973 60 151M = 166176 354 CAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCGCATTCATATCATTAGGTATGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTAGCAGGGCAGACATC FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:3 MD:Z:41A94G0A13 MC:Z:151M AS:i:136 XS:i:42 A00126:224:H7HMWDSX3:3:1111:23647:16172 145 Test_seq 166176 60 151M = 165973 -354 CTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:48G102 MC:Z:151M AS:i:146 XS:i:50 A00126:224:H7HMWDSX3:3:1111:27841:16454 65 Test_seq 169311 60 151M = 39072 -130240 TTCTTTAGTCAGTCTGGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:::FF:F:FFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:1 MD:Z:15A135 MC:Z:151M AS:i:146 XS:i:116 A00126:224:H7HMWDSX3:3:1111:27841:16454 129 Test_seq 39072 38 151M = 169311 130240 GAAATTCATAGAATGCAATGAGAATGAAAATACTTCCTATCAAAACGTTTGGGACACAGTGAAAGCAGGGCTCAGAGGTCAATTTATATCAATAAATGCACACATACATAAAGAAGAAAGAGCCAAAATCAGAGAACTGTCCCTATAACTT FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:11 MD:Z:14T3C4C6C15C11A9T15C0G45A13C5 MC:Z:151M AS:i:96 XS:i:72 A00126:224:H7HMWDSX3:3:1111:5692:17863 97 Test_seq 168773 60 151M = 169099 461 GATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTGGTATCAGGGAGATGGT FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:141A9 MC:Z:135M16S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1111:5692:17863 145 Test_seq 169099 54 135M16S = 168773 -461 GTTATGCGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTGAGAGTACAATTTTTCGTAGTAATCTGAAATGATTAATTGTGGAAAGTATA FFF:FFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:6G55G1A35T1C11A20 MC:Z:151M AS:i:105 XS:i:76 A00126:224:H7HMWDSX3:3:1111:10402:18474 97 Test_seq 165665 60 151M = 165955 441 TCAAGTGAACATTTACCATTCCAATCAGTCTGTCACATGTAGGTTTACATACATCATACTCCCTTCTCCCACTTGCTCTCCCCCTATTGAGTCAACCCTTTCAGTCTCTCGTTTCCTGCCAATTTTGCCATCTTCCCTCTCTCTCTATCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF,FFFFF:FFFFFFFF,FFFFFF:,FF::FFF:FFFFFFF:FFFF: NM:i:4 MD:Z:80G13G20G19T15 MC:Z:151M AS:i:131 XS:i:0 A00126:224:H7HMWDSX3:3:1111:10402:18474 145 Test_seq 165955 60 151M = 165665 -441 GTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTAGTCACATTCATATCATTAGGTATGGTCTTTTTATGAGAATTTGGGGTCTGTATCCCATTGGCCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTC F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:117T33 MC:Z:151M AS:i:146 XS:i:35 A00126:224:H7HMWDSX3:3:1111:13024:18787 97 Test_seq 170380 60 151M = 170580 339 ACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTT FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF,FFF,F::FFFF:FFF,FFF,FFF:FFFF NM:i:1 MD:Z:110T40 MC:Z:14M12I125M AS:i:146 XS:i:48 A00126:224:H7HMWDSX3:3:1111:13024:18787 145 Test_seq 170580 60 14M12I125M = 170380 -339 GTTGAGTCGTTACGTTTTTCTTGGCTTTTTTCTTGAGTTATGGAGTTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCATTCTATATCGCCTTGTATCACTCTCCATCTGGCAGT FFFFFFFFFFFF,F::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:16 MD:Z:32A69G2G6A26 MC:Z:151M AS:i:105 XS:i:37 A00126:224:H7HMWDSX3:3:1111:26612:18834 97 Test_seq 212573 50 151M = 212858 436 GCTCTTATTCAACATTGTGCTAGAAGTCCTAGCCAGAGCAATTAGGCTAGACAAAGAAATAAAGGGCATCCGGATTGGCAAGGAGGAAGTAAAATTATCTCTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:4 MD:Z:11G12G45T68T11 MC:Z:151M AS:i:131 XS:i:105 XA:Z:Test_seq,+40248,151M,10; A00126:224:H7HMWDSX3:3:1111:26612:18834 145 Test_seq 212858 60 151M = 212573 -436 AGTAGCCCCCAAGAAGATAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAATTACAAAGTACTACCACAAGAAACTAAAAAGGACCTACTTAAGTGGAAAAACATACCTTGCTCATGGATAGGAAGACTT FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:86T64 MC:Z:151M AS:i:146 XS:i:85 A00126:224:H7HMWDSX3:3:1111:9417:19272 97 Test_seq 221904 12 21M130S = 305995 84242 ATTTCCTTTGCCCATTTTTTAGATCAAAAGGAAGAGAGAGATATTTACAATGTCTTTTCTAATTTCTGAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFF,FFFFF: NM:i:0 MD:Z:21 MC:Z:151M AS:i:21 XS:i:0 SA:Z:Test_seq,305900,+,20S131M,60,0; A00126:224:H7HMWDSX3:3:1111:9417:19272 2145 Test_seq 305900 60 20H131M = 305995 246 AGATCAAAAGGAAGAGAGAGATATTTACAATGTCTTTTCTAATTTCTGAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGAT FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFF,FFFFF: NM:i:0 MD:Z:131 MC:Z:151M AS:i:131 XS:i:0 SA:Z:Test_seq,221904,+,21M130S,12,0; A00126:224:H7HMWDSX3:3:1111:9417:19272 145 Test_seq 305995 60 151M = 221904 -84242 TTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATACTAAAGAGGCAAAAGTCATGGAAACCACTGGAGGGCCATTATCTTTCCTCATCTCCCCCATGTTGCCCCATCTCATCACAAAGGTCTGCGGTTGGTAAAGAGAGGAACTGGATG FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:21M130S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:17526:19288 97 Test_seq 152698 60 151M = 152848 286 ATCCCCTTTATTTCTTTATCTAGCCTAATTGCTCTGGCTAGGACCTCCAACACAGTGTTGAATAAGAACGGTGATAAAGGGCATCCTTGTCTGGGTCCCAGTCTCAGTGGGAATGCTTTCAGGCTCTCTCATTTTGGATTATGTTGGCTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFF:FFFF,:FFFFFFFF,FFF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:FFFF:,FFFFFFFFFFF NM:i:1 MD:Z:139G11 MC:Z:136M15S AS:i:146 XS:i:79 A00126:224:H7HMWDSX3:3:1111:17526:19288 145 Test_seq 152848 45 136M15S = 152698 -286 TTGGCTTTGTATAAATGCCCTTTATTATGTTGAGGAATTTTCCTTCTATTCCTATTTTGCTGAGAGTTTTTATCATGAATGGGTGTTGAACTTTGTCAAATGCCCTTTTTGCATCAGTTGATAATATCTTGTGATTGATCACTTTTGGTAG FFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFF:FFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:136 MC:Z:151M AS:i:136 XS:i:116 XA:Z:Test_seq,+40033,15S136M,4; A00126:224:H7HMWDSX3:3:1111:11062:21277 97 Test_seq 170255 60 151M = 170474 348 GCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATT FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:7A143 MC:Z:129M22S AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1111:11062:21277 145 Test_seq 170474 60 129M22S = 170255 -348 ATGTGTGTTGTTCGCAATTTCATTTTTCCACATACTTTTTTGTGCTGAGACATTTTTCTTAGTAAATTGTGAGATCTTCATTTTCATAGTGTTTGATTTTATGTTTGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATGGAG FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:9 MD:Z:12G2C0T34G12G11C11A5A1C32 MC:Z:151M AS:i:84 XS:i:0 A00126:224:H7HMWDSX3:3:1111:9127:26193 81 Test_seq 171316 60 151M = 171173 -294 TTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTCTCCCCCCTCCCTGTTCTGGGACTACAATCACCCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:82G1G15T5C7T36 MC:Z:151M AS:i:126 XS:i:41 A00126:224:H7HMWDSX3:3:1111:9127:26193 161 Test_seq 171173 60 151M = 171316 294 GATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFF,F:,FFF::FF:FFFFFFFFF:FF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:36 A00126:224:H7HMWDSX3:3:1111:3956:26381 113 Test_seq 212649 27 151M = 168465 -44185 GGCAAGGAAGAAGTAAAGTTATCACTATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGAT FFF:,:FF:FFF:FFFFFFFFFF:FF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFF NM:i:7 MD:Z:8G8A5T20C0T17T61G25 MC:Z:151M AS:i:116 XS:i:101 XA:Z:Test_seq,+168239,151M,10; A00126:224:H7HMWDSX3:3:1111:3956:26381 177 Test_seq 168465 60 151M = 212649 44185 CGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTT FFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:30T120 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1111:9986:26334 65 Test_seq 213148 3 151M = 167568 -45581 GCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:1T149 MC:Z:151M AS:i:149 XS:i:146 XA:Z:Test_seq,-167740,151M,1; A00126:224:H7HMWDSX3:3:1111:9986:26334 129 Test_seq 167568 9 151M = 213148 45581 GATCGACTTGCAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAGAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFF:FFFFFFF:FFFFF,FFFFFFFFFFFFFFFFF:FFFFFFF NM:i:3 MD:Z:4C5G87A52 MC:Z:151M AS:i:136 XS:i:130 XA:Z:Test_seq,-213320,140M2I9M,5; A00126:224:H7HMWDSX3:3:1111:3043:26428 97 Test_seq 170295 60 151M = 170622 468 TTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTT :FF:FFFFFFFF,FF:FFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFF:F:FFF:FFFFFFFF:FFFFF,:FF,FF:FFFFFFFFFF,FF,FFFFFFFFF,FFFFFF,:F,FFFFFFFF:FF NM:i:1 MD:Z:81C69 MC:Z:141M10S AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1111:3043:26428 145 Test_seq 170622 60 141M10S = 170295 -468 CTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCAATGCCTCCTGTATTTAGTCCCTCTTTTTGATTATTGTGATCTCTTACCTAT F,F,FFFF,FFFF,FFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFF:::FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF NM:i:3 MD:Z:63G6A18C51 MC:Z:151M AS:i:126 XS:i:37 A00126:224:H7HMWDSX3:3:1111:10646:27853 65 Test_seq 213148 3 151M = 167568 -45581 GCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFF NM:i:1 MD:Z:1T149 MC:Z:151M AS:i:149 XS:i:146 XA:Z:Test_seq,-167740,151M,1; A00126:224:H7HMWDSX3:3:1111:10646:27853 129 Test_seq 167568 9 151M = 213148 45581 GATCGACTTGCAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAGAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,F:FF,FF:FFFFFF,FFF:F:FFF:F:FFFFFFFFFF::,FFFFF, NM:i:3 MD:Z:4C5G87A52 MC:Z:151M AS:i:136 XS:i:130 XA:Z:Test_seq,-213320,140M2I9M,5; A00126:224:H7HMWDSX3:3:1111:23954:28009 97 Test_seq 166002 60 151M = 166206 355 ATTCCTTTAGTTGCAGTCATACCATTAGGTATGGTCTTTTCATGAGAATTTGGGGTCTGTTTCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:6C4C0A2T5T18T19A90 MC:Z:151M AS:i:120 XS:i:0 A00126:224:H7HMWDSX3:3:1111:23954:28009 145 Test_seq 166206 60 151M = 166002 -355 GTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAG FFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:18G132 MC:Z:151M AS:i:146 XS:i:81 A00126:224:H7HMWDSX3:3:1111:22354:28025 81 Test_seq 169277 60 151M = 169011 -417 TTCTCATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGC FFFFFFFFFFFFFFFFFF::FF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:1 MD:Z:4T146 MC:Z:151M AS:i:146 XS:i:98 A00126:224:H7HMWDSX3:3:1111:22354:28025 161 Test_seq 169011 60 151M = 169277 417 GATTTGGTAGAACTCTGCAGTGAAGCCGTCCAGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACTGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCC FFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFF,FFFFF,:FFFFFFF,F,F:FFFFFFFFF:FFFFFFFFFFF,FFFFFFFFF,FFFF:FFFFFFFFFF NM:i:5 MD:Z:1G28T0G37C80G0 MC:Z:151M AS:i:133 XS:i:58 A00126:224:H7HMWDSX3:3:1111:9661:28714 97 Test_seq 125729 60 151M = 125939 361 TTTGCAGCTTTCCCAAGGCAGAGCAAAGCACGAGAAGATGGGCTCACAGGCCCGATCGCAGGGGCTCACAGCCTCCTCACTCTCCTAGGTTGCATTTCACAGACCAGCTTCCCTCACCCAGGATACTGGAGGCCGCAGCCTTCCAAATCAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:9661:28714 145 Test_seq 125939 60 151M = 125729 -361 GTGATTAACCTCATTAGCACACGTGGTTGCCTCAGATGGCATTAGCAGTCAGCCAGATATTATTAAAAACAACAGCCATTGCCCTGTTGGGTGCACCGTGCCTGGTGCTCCCTAGCTCTGGGCCTTCAGGAGCAGCTGGTCACACACCTTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:19542:29011 97 Test_seq 170945 39 98M1D53M = 171174 380 CAAACAATATCCTTTAGTATTTCTTGTAGCTTTGGTTTGTTTTTTTCAAATTCTCTAAACTTGTGTTTGTCTGTAAATATCTTAATTTTGCATTCATATTCAGAGAGAGTTTTGCTGGATATATGATCCTTGGCTGGCAGTTTTTATCCTT FFFFFFF:FFFF,FFFFFF:FF:F:FF:FF,FF:FFF:FF,FFFF,FFFF,:,FFFFFFFFFFF,FFFFFF,FFFFFFF::FFF:FFFF:F,,FFFFF,F,FFF,FFFFFFF,FFFFFFFFFF:,FF:FF:F,FF:FFF,F:,FF,:F:FF NM:i:16 MD:Z:8C5A2C8C2T0C7A0G5G22A19C0T1C6^T3C31T17 MC:Z:151M AS:i:69 XS:i:40 A00126:224:H7HMWDSX3:3:1111:19542:29011 145 Test_seq 171174 60 151M = 170945 -380 ATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGA FFFFFFFFF:F,FFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFF,FFFF:FFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:98M1D53M AS:i:151 XS:i:37 A00126:224:H7HMWDSX3:3:1111:27706:33755 97 Test_seq 138290 60 151M = 138556 417 GCTGAGTAACCAGTAGTCTATTAAGAAGGCACATATGGTGCTAGAATAACTAGAAAATAGTGCTGGAAAAACTGCATATCCATATGGAAAAAATGAACCTTAACCCTTACTTCACACCATATATAAAAACCAATTCAAAACAGATCTTAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFF: NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:27706:33755 145 Test_seq 138556 60 151M = 138290 -417 AAGAACAGGAAAGGCACCTTTATTTTGTTGTACAAGGAAAAGGAGTCAGTCGGAGCTGTTGCGCTAAGACTGCTCCCCAAGGACAAGGGGAAAAGTTACTTTTAAGGGGTTTACAAGAAGGGAGTTCATACGAGTTATATCAGCAACATTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:8910:34209 97 Test_seq 167531 59 31S120M = 167558 178 GCCATCTTGGTAACTCTTTGGAAACTATGACTGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCC FFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF: NM:i:0 MD:Z:120 MC:Z:151M AS:i:120 XS:i:95 A00126:224:H7HMWDSX3:3:1111:8910:34209 145 Test_seq 167558 32 151M = 167531 -178 TTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTC FFFFFFFFFFFFFFF:FFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:31S120M AS:i:151 XS:i:136 XA:Z:Test_seq,+213330,130M2I19M,3; A00126:224:H7HMWDSX3:3:1111:24777:34413 97 Test_seq 417145 60 151M = 417457 463 CATCTTTCTTACATTTCAAAGTCTGCTCCCTGGTTAAAGGAAGTGGAAATAAAGCGTGGGATTTGGGGATTAAATCAGTGGGATTCCAAATTAGGCTTATTTTTTTGTGGTGGTTATTCAATTACATAAATCTGTACTCTTGATCTTCTCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:24777:34413 145 Test_seq 417457 60 151M = 417145 -463 TTCCAAAATCCAGAAGTTCTGGAAAAGAGATTTTTTTCTCGGCAGCCCCAAAATCCAAAAAATTTTTGGAGGAAAAAAAAAGTCCTTTGTAAGGTTGGCACCAAAACTTAGTCGACTGCAAAAACCTGACTTGAACTGATGTTCACACTTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:46T104 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1111:27832:34663 97 Test_seq 167854 60 151M = 168197 494 CCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTGCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTG FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:59C91 MC:Z:151M AS:i:146 XS:i:59 A00126:224:H7HMWDSX3:3:1111:27832:34663 145 Test_seq 168197 37 151M = 167854 -494 TGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTACGTATACGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:4 MD:Z:74T64C1T7G1 MC:Z:151M AS:i:134 XS:i:115 XA:Z:Test_seq,+212691,151M,8; A00126:224:H7HMWDSX3:3:1111:8802:34773 81 Test_seq 168440 60 151M = 168347 -244 CTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:29S122M AS:i:151 XS:i:83 A00126:224:H7HMWDSX3:3:1111:8802:34773 161 Test_seq 168347 60 29S122M = 168440 244 TTATCCCCAATTTAGGTTTATTTGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF NM:i:2 MD:Z:18A28T74 MC:Z:151M AS:i:112 XS:i:66 A00126:224:H7HMWDSX3:3:1111:1570:34773 97 Test_seq 72280 60 151M = 72488 359 ATCAGTCTGTTTTGAGATAGAAAAGAGATTAAACAAGGAGATTAAAGAAGCAGCGATGGAGGAAGAGAGATGCAAGGCCGCATGAAGGTCGCCCAAGAGCAGAAGCTCAAAAGAGGCTAAGGTCTTCCTCCAGAGCTGAGATAGAAAGCTT FFFF:FFFFFFFFFFFFFF:FFFFF::FFFFF:FFF:FFFFFFFF:FFFFFFFFFF:FF:FFFF::FF:FFF:FFFF,FFFFFFFFFFFF::FFFF::FFFF:FFFFFFF,F:,FF:FF:FFFFFFFF:FFFFFF:FFFFFFFFFF:FF:F NM:i:1 MD:Z:137A13 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1111:1570:34773 145 Test_seq 72488 60 151M = 72280 -359 AAATTTGTATGTTAAAACCGCCTACTTGTGGTATTTCTGTTATAGAACACTGGATAACTAGGACAGTTGCCGTGAAGGGGCAGCCGACTCGATGGCAGCCAAAAACAACAACAACAGCAGTGAAACACAGGTAGTGTTTCTTGGATTCATT F:FFFFFFF:,F:FFFFFF:,FFFFFF,F,F:,FF:FFFFFFFFFFFFFFF,FFFFFFFF:FFFF:FFFFFFFF:FFFFFFF:F,FFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFF:F:::FF:FFFFFF: NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1111:14064:36808 97 Test_seq 167148 42 151M = 400900 233887 AATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF,,F:FFF,,FFF:FFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFF NM:i:1 MD:Z:95T55 MC:Z:135M16S AS:i:146 XS:i:126 XA:Z:Test_seq,-213736,151M,5; A00126:224:H7HMWDSX3:3:1111:14064:36808 145 Test_seq 400900 4 135M16S = 167148 -233887 TTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCCTTCCCCACTC ,FF:FFFF,FFFFFFFF,FFFF:FF:FF,F:FFFFFFFFF:FFFFF:FF:FFF:FFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFF:FFFFFFFF:FFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:FFFF,F:F NM:i:7 MD:Z:4T0T11G32G19A0C8T54 MC:Z:151M AS:i:104 XS:i:100 XA:Z:Test_seq,-167437,135M16S,7; A00126:224:H7HMWDSX3:3:1112:23149:2597 97 Test_seq 169079 37 39M4I108M = 169318 390 CCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGATCGATCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTG FFF:FFF:FFFFF,FFFFFFFFFFFFFFF:F:FFFFFFFFFFF,FFFFFFF:FF:F:F:F::FFFFFF:FFFFF:FFFFFFF:FFFFF,FFF,F:FFFFFFFFFFFFFFF,FF:F:FFFFFFF:FF,FF,FFFFFF,:,,F:F:,F:F,:F NM:i:12 MD:Z:40T3A37G1A37C11A1G2G7 MC:Z:151M AS:i:99 XS:i:76 A00126:224:H7HMWDSX3:3:1112:23149:2597 145 Test_seq 169318 60 151M = 169079 -390 GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT FFFFFFFFFFFFFFFFFF,FFFF:FFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF NM:i:1 MD:Z:149G1 MC:Z:39M4I108M AS:i:149 XS:i:112 A00126:224:H7HMWDSX3:3:1112:10447:5243 81 Test_seq 168773 60 121S30M = 168427 -376 TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCGATCAGGGTGAATTATTTTTTTGATGTGTT FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF NM:i:0 MD:Z:30 MC:Z:151M AS:i:30 XS:i:0 SA:Z:Test_seq,168555,-,121M30S,60,1; A00126:224:H7HMWDSX3:3:1112:10447:5243 2129 Test_seq 168555 60 121M30H = 168427 -249 TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:25A95 MC:Z:151M AS:i:116 XS:i:70 SA:Z:Test_seq,168773,-,121S30M,60,0; A00126:224:H7HMWDSX3:3:1112:10447:5243 161 Test_seq 168427 60 151M = 168773 376 GCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCAGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGT FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:68T82 MC:Z:121S30M AS:i:146 XS:i:43 A00126:224:H7HMWDSX3:3:1112:21884:7012 97 Test_seq 167977 26 151M = 168170 344 AGTGCGTTAAATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGCAGTAGAGCTTTGTAGTTTTCTTTGTATAGGTCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFF NM:i:8 MD:Z:9T64G8C32T2C4T3A13G8 MC:Z:151M AS:i:111 XS:i:96 XA:Z:Test_seq,-212911,141M10S,9; A00126:224:H7HMWDSX3:3:1112:21884:7012 145 Test_seq 168170 31 151M = 167977 -344 TGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:101T49 MC:Z:151M AS:i:146 XS:i:131 XA:Z:Test_seq,+212718,151M,4; A00126:224:H7HMWDSX3:3:1112:16595:8124 81 Test_seq 170307 60 151M = 170144 -314 TGTGTCTCTAAGTCTACGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTC FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F NM:i:1 MD:Z:16A134 MC:Z:16S135M AS:i:146 XS:i:48 A00126:224:H7HMWDSX3:3:1112:16595:8124 161 Test_seq 170144 60 16S135M = 170307 314 TTTTATGTTATTGATCGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTT FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:FFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFF:FFF,FFFFFFFF:FFFFFF:F,FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFF,F NM:i:3 MD:Z:51C0A65A16 MC:Z:151M AS:i:120 XS:i:53 A00126:224:H7HMWDSX3:3:1112:9209:9361 97 Test_seq 212679 60 151M = 212803 275 GCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:1 MD:Z:33T117 MC:Z:151M AS:i:146 XS:i:110 A00126:224:H7HMWDSX3:3:1112:9209:9361 145 Test_seq 212803 58 151M = 212679 -275 TCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGAGAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAACTACAAAGCACTACTACAAGAAAT FFFFFFF:FFFFFFFFFFFFFFFFFFFF:::FFFF,F,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:34A37T54T7T14C0 MC:Z:151M AS:i:130 XS:i:100 A00126:224:H7HMWDSX3:3:1112:16875:9518 81 Test_seq 398018 60 151M = 397769 -400 GACAAACTGACAGACTAGTGGTGGATTTCTAAATCATACCACTCAAAAAGCCATATATTATCATGTGAAATTTTTTTTCACAGTTTATGAATGAAACTTTTTCTTTCTACCCTTGCCAACACTGAATAGTATCAAGATTTTTCAATTTGCC FFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:134C16 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1112:16875:9518 161 Test_seq 397769 60 151M = 398018 400 CGTCTTTTGTGTGCCTTCCAACCTGAGGGGCTCACCTTACTGTACTATATCAGACAATGTTCTGCTGCTATTCATCAGCTTTTCAGTGGCCTAATTTTTTTCAGAAGTAGATCACCAGGTCCTTCTTCCCAGTCTGTCTTAGTCTGGAAGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1112:10357:9721 97 Test_seq 212679 60 151M = 212803 275 GCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF NM:i:1 MD:Z:33T117 MC:Z:151M AS:i:146 XS:i:110 A00126:224:H7HMWDSX3:3:1112:10357:9721 145 Test_seq 212803 58 151M = 212679 -275 TCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGAGAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAACTACAAAGCACTACTACAAGAAAT F:FFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:34A37T54T7T14C0 MC:Z:151M AS:i:130 XS:i:100 A00126:224:H7HMWDSX3:3:1112:22914:10739 81 Test_seq 167513 57 151M = 167173 -491 ATCCTATTTTTTCTTCCATGATCTTTATTGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:28C122 MC:Z:151M AS:i:146 XS:i:119 XA:Z:Test_seq,+213375,85M2I23M2I37M2S,6; A00126:224:H7HMWDSX3:3:1112:22914:10739 161 Test_seq 167173 50 151M = 167513 491 GATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGC F:FFF,FFFFFFFFFFFFF:FFFFF:::FF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFF:,FFFFFFFFFFFFFFFFF:FFFF:FFFF,FFF:FFF:FF:FFFFFFF:,:FFFFFF,FFFFFFFF:FFFF:FFFFF,FFFFFF:FF NM:i:2 MD:Z:70T79A0 MC:Z:151M AS:i:145 XS:i:121 XA:Z:Test_seq,-213711,151M,6; A00126:224:H7HMWDSX3:3:1112:6008:14027 97 Test_seq 168347 60 18S133M = 168522 326 GCTGCCAAATCACTGAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCACGATGTTGAATAAGAGCGGTGATAAAGGGCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:18A81T32 MC:Z:151M AS:i:123 XS:i:76 A00126:224:H7HMWDSX3:3:1112:6008:14027 145 Test_seq 168522 60 151M = 168347 -326 CTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCGTTTTCTGCATCTATTGATAAG FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:129C21 MC:Z:18S133M AS:i:146 XS:i:72 A00126:224:H7HMWDSX3:3:1112:27778:14184 97 Test_seq 170407 60 151M = 170510 254 AGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATGCTTTTTTGTGCTGAGACGTTTTTCTTGGTAGATTGTGAGATCCTCATTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,,FFF,FF,FFF,,,F:F::,,F,F:FF:FFF NM:i:2 MD:Z:100A26A23 MC:Z:151M AS:i:141 XS:i:25 A00126:224:H7HMWDSX3:3:1112:27778:14184 145 Test_seq 170510 60 151M = 170407 -254 TTTTTGTGCTGAGACGTTTTTCTTGGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTCGTTACGTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:24A126 MC:Z:151M AS:i:146 XS:i:26 A00126:224:H7HMWDSX3:3:1112:5927:14763 97 Test_seq 366670 60 151M = 366905 386 AGTGTTAAAAAGCAAAGATGTTACCTTGAAGACTAAGGTGTGCCTGACCGAAAAAAAGACCGAAGCCATGGTATTTTCAATTGCATCATATGCATGTGAAAGCTGGACAATGAATGAGAAAGGCCAAAGAAGAATTGATGTTTTTGAATTA FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:50 A00126:224:H7HMWDSX3:3:1112:5927:14763 145 Test_seq 366905 60 151M = 366670 -386 TCCTTAGAAGCAAGATTGTCAAGACTTTGATGTACTTTGGGCATGTTATCAGGAAGGATTCATCCCTAGAGAAGGACACCATGCTTGGTAAAATAGAAAAAAACCAAAACCAAACCCACTGCCGTCAAGTGATTCCGACTCATAGCGATCT F:FFFFFFF::FF:FFFFFFFF:F,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF,FFFF,FFFFFFFF,FFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:61G89 MC:Z:151M AS:i:146 XS:i:22 A00126:224:H7HMWDSX3:3:1112:9254:15922 97 Test_seq 260973 8 53M = 260973 53 GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCTGTTGTAGAAGGGCAC FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FF:FFFF,,,FFFFF NM:i:3 MD:Z:37G7C0T6 MC:Z:53M AS:i:40 XS:i:36 XA:Z:Test_seq,+347640,45M8S,2; A00126:224:H7HMWDSX3:3:1112:9254:15922 145 Test_seq 260973 34 53M = 260973 -53 GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCGGTTGTAGCTGGGCAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:53 MC:Z:53M AS:i:53 XS:i:43 XA:Z:Test_seq,-150325,53M,2; A00126:224:H7HMWDSX3:3:1112:32723:16391 81 Test_seq 171731 60 151M = 171626 -256 TCAGTGTGTTCCTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGG F,FF:FFF,FFF:FFFFFFFFFFFFFFF:F:FFFF,FFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFF,FFFFFFF:F:F:,:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFF:,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF NM:i:1 MD:Z:27G123 MC:Z:27S124M AS:i:146 XS:i:25 A00126:224:H7HMWDSX3:3:1112:32723:16391 161 Test_seq 171626 60 27S124M = 171731 256 TTCAATAAAAACCCAAATGAAATCGATTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTGTTAATTTTTCCACTATGTTCTTGAATAATCTTTTTGAGTTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTT FFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF:,FFFFFF,F:,FFFFF:FFFFFFFF:F,FFFFFFFFF:FF,FF,FFFFFF,FFFFFFFFFF,,:F:,FFFFF,F:FF:,,F,FFF NM:i:4 MD:Z:50A14C13C11T32 MC:Z:151M AS:i:104 XS:i:71 A00126:224:H7HMWDSX3:3:1112:27633:17722 113 Test_seq 385655 30 127S24M = 388598 3071 AAGTGCGTCAGAAGAAAGTCTGCAAAGCATTTCAGAGAGCACTCAAAGAGAAGCCTTTTAAGGAAAACTCTGAACAAAAATTATCCTCATAAGATAATTCTCTCTTCAACACTCCTCATTCTGGAGTAATCACAGACTGTTCACACATTGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:0 MD:Z:24 MC:Z:151M AS:i:24 XS:i:0 SA:Z:Test_seq,388349,+,24S127M,60,0; A00126:224:H7HMWDSX3:3:1112:27633:17722 2145 Test_seq 388349 60 24H127M = 388598 400 ACTCCAGAATGAGGAGTGTTGAAGAGAGAATTATCTTATGAGGATAATTTTTGTTCAGAGTTTTCCTTAAAAGGCTTCTCTTTGAGTGCTCTCTGAAATGCTTTGCAGACTTTCTTCTGACGCACTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:127 MC:Z:151M AS:i:127 XS:i:0 SA:Z:Test_seq,385655,-,127S24M,30,0; A00126:224:H7HMWDSX3:3:1112:27633:17722 177 Test_seq 388598 60 151M = 385655 -3071 AGATTGGCCATGAATGGGGTGATAAGAATAGGGAGAAGCACATACCAGTAGACCAGACTTCCTGTGCACAAGCCTTTGAATCCTGCTTCTCGCTTCTGAAATAGCATTCATCTTAGGGCTGGGATCATTTATTCTCTCTGGAATTAAGCAA FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:127S24M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1112:25825:18098 81 Test_seq 171461 60 151M = 171174 -438 ATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATT FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:122T28 MC:Z:33S118M AS:i:146 XS:i:39 A00126:224:H7HMWDSX3:3:1112:25825:18098 161 Test_seq 171174 60 33S118M = 171461 438 TATATTGAGTATATCATCATTTGACCTCAAAGAATTCTCCCTTGAAGGAAACCTTTTTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAAT FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFFFFFFF,F:FFFFFFFFF NM:i:1 MD:Z:23C94 MC:Z:151M AS:i:113 XS:i:35 A00126:224:H7HMWDSX3:3:1112:9362:18740 81 Test_seq 170592 60 151M = 170285 -458 CTTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCGATGCCTCCTATATTTAGTCCC FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,FFFFFFFF:FFFF,FFFFF:F,FFFFFF:FFFFF:FFFF:FFFFFFF,FFFFFFFFF NM:i:8 MD:Z:1G45A42G2G3A2A28A9G11 MC:Z:151M AS:i:114 XS:i:24 A00126:224:H7HMWDSX3:3:1112:9362:18740 161 Test_seq 170285 60 151M = 170592 458 ATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCCTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGA FFF,FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:,FF::FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFF:FFFF:FFFFFFFFF:FFFFF:FFFF,F::FFF,F:FFFFFF,F,:FFFFFFFFF,FFF,FFFFF NM:i:1 MD:Z:107T43 MC:Z:151M AS:i:146 XS:i:82 A00126:224:H7HMWDSX3:3:1112:31421:19304 97 Test_seq 236629 60 151M = 236976 498 AAGAAAAATAAAAGTAAATTCCACAGTAAGGTGAACAATTAAGATGAGATTACCAACTGAGATTACCCATTTCAGAAAGAAGTATTCTAAGTAAAAGGTAATCAAAAGTTGGCTTTTCTACTTTCAATAGAGGATTCACACATAGTCATTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1112:31421:19304 145 Test_seq 236976 60 151M = 236629 -498 TAAAATACATGCAGCAGAAGGTTAGTACGTAAAGACTAATTCAAACTATGCACTGATGTGTTGTTGTTAGCTGCTGTCAGGTTGGCCCTGACTCATGGTGATGCCACGCACAATGGAACAAAAGGCTGCCAGGTCCTGTGTTATATCCCAT FFF:::FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:28 A00126:224:H7HMWDSX3:3:1112:21314:19586 81 Test_seq 166369 60 151M = 166044 -476 CATTGACTTAGAAGTCCCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCATTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAGTTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:5 MD:Z:1G14T52T42A23T14 MC:Z:151M AS:i:129 XS:i:30 A00126:224:H7HMWDSX3:3:1112:21314:19586 161 Test_seq 166044 60 151M = 166369 476 TGAGAATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGTACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F NM:i:1 MD:Z:93C57 MC:Z:151M AS:i:146 XS:i:76 A00126:224:H7HMWDSX3:3:1112:25563:19680 97 Test_seq 95887 38 102M = 95891 102 TACTTGTGGTCCATTAGTCCTTTGGACTAATCTTTCCCTTGTGTCTTTGGTTTTCTTCATTTTCCCTTACTCCAGACGAGGTGAGACCAGTGGAGTATCTTA FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:F::FFFFFF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFFF:FFFFFFFFF,FF:FFFFFFFFFFFF NM:i:0 MD:Z:102 MC:Z:4S98M AS:i:102 XS:i:87 XA:Z:Test_seq,+81335,102M,3; A00126:224:H7HMWDSX3:3:1112:25563:19680 145 Test_seq 95891 1 4S98M = 95887 -102 GTGGTGTGGTACATTAGTCCTTTGGACTAATCTTTCCCTTGTGTCTTTGGTTTTCTTCATTCTGCCTTGCTCCAGATGGGGTGAGACCAGTGGAGTATCTTA :,,:F:FFFF,FF:F:FFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFF,FFF:FF,FFFF:FFFF,F,FFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:6C50T1C4A7C1A23 MC:Z:102M AS:i:68 XS:i:66 XA:Z:Test_seq,-81346,11S91M,5; A00126:224:H7HMWDSX3:3:1112:14479:19899 97 Test_seq 166210 60 151M = 166417 357 TTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATT FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFF:FFFF,:FFFFFFFFFFFFFFF:FFF,FFF:FFFFFF:FFFFFFFFFFFFFF:F NM:i:0 MD:Z:151 MC:Z:140M1I10M AS:i:151 XS:i:33 A00126:224:H7HMWDSX3:3:1112:14479:19899 145 Test_seq 166417 60 140M1I10M = 166210 -357 TGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCTGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATAAATAAAAAAACCCT FFFFFFFFFFFFF,F,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:34C101C13 MC:Z:151M AS:i:136 XS:i:30 A00126:224:H7HMWDSX3:3:1112:31213:20290 81 Test_seq 172001 60 127M4I20M = 171742 -406 AAAACGGTCGCTGTCTGCCCGTGTTTGTTCGTTTTCCGTCTCTAAGTCTGTGCTTGTTGTTCAGAGTTCGTAGATTGTTATGTATGTGATCGATTCACTTGTTTTTCCGAGTCTTTGTTGCACGAGGGATCGATCCGCGGTAGCGTCCACC FF::FFFFFFF,FFF:FFFFFFFF,FFFFFF::,FFFFFFFF,F:FFF,F:FFFFFFFFF:F:FFFFF:FF,FFFFFFFF,FF,,F,F::FFFF,FFFFFFFF:FFFF,FFFFF:,F:FFFF,FFFFFFFFFFFFFFF:F,FFFFFFF:FF NM:i:13 MD:Z:4T1A5A9A10C2T15T28A40A24 MC:Z:151M AS:i:95 XS:i:0 A00126:224:H7HMWDSX3:3:1112:31213:20290 161 Test_seq 171742 60 151M = 172001 406 CTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCT F,FFFFFF:FFFFFFFFFF:FFFF::FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFF:FFFFF,FFFFFFFFFFFFFFFFFF,::FFF:F:FFFFFFFFFFFFF,FF NM:i:1 MD:Z:16G134 MC:Z:127M4I20M AS:i:146 XS:i:25 A00126:224:H7HMWDSX3:3:1112:3341:20932 65 Test_seq 214068 60 151M = 166640 -47429 AACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAATGGA FFFFFFFFFFFFFFF:FFFFFFF:FFFFF:FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:F:FFFFFFF:FFFFFFFFFFFFFFF:F:FFF NM:i:0 MD:Z:151 MC:Z:15S136M AS:i:151 XS:i:72 A00126:224:H7HMWDSX3:3:1112:3341:20932 129 Test_seq 166640 2 15S136M = 214068 47429 AAATAAAACAGGATCGATCTTATAATAGTGGTCTTATACAATATTTGTCCATTTGCCTCTGACTAATTTCGCTCAGCATAATGCCTTCCAGGTTCCTCCATGTTATGAAATGTTTCATGGATTCGTCACTGTTCTTTATCGATGCTTAGTA FFFFFFFFFFF:FFFFFF:FFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:F,FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:8 MD:Z:35T13C5A12T7A25G0A26G5 MC:Z:151M AS:i:96 XS:i:93 XA:Z:Test_seq,-214259,126M1I9M15S,8;Test_seq,+400061,15S102M34S,5; A00126:224:H7HMWDSX3:3:1112:28664:21418 97 Test_seq 165351 60 23S128M = 165635 435 ACGCTGCCCGGCCAAATCCGATCAATCCACCAGGTGCTCCTTGGAAACCCTATGGGGCAGTTCTACTCTGTCCTATAGGGTTGTTATGAGTTGAAATCGACTTGATGGCAATGGGGTTTTTTACGGTGGCTTTGTTTTTTTTTTTTTTTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,,FFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFF,,,F:FFF:F,FFFFFFFFF: NM:i:0 MD:Z:128 MC:Z:151M AS:i:128 XS:i:46 A00126:224:H7HMWDSX3:3:1112:28664:21418 145 Test_seq 165635 60 151M = 165351 -435 TTTTTTTTTTTAATTAATTTTTATTAAGCTTCAAGTGAACATTTACCATTCCAATCAGTCTGTCACATGTAGGTTTACATACATCATACTCCCTTCTCCCACTTGCTCTCGCCCTATTGAGTCAGCCCTTTCAGTCTCTCGTTTCGTGCCA FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:23S128M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1112:10158:21809 97 Test_seq 169288 60 151M = 169528 391 GTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTA FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFF NM:i:1 MD:Z:21T129 MC:Z:151M AS:i:146 XS:i:106 A00126:224:H7HMWDSX3:3:1112:10158:21809 145 Test_seq 169528 60 151M = 169288 -391 CTTTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCC FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:29C70T11A22G15 MC:Z:151M AS:i:131 XS:i:88 A00126:224:H7HMWDSX3:3:1112:9896:22106 97 Test_seq 170144 60 23S128M = 170285 292 TTTCTGTCAATGGAAATATATTAGATCAATTGTCCCTTTTGTCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGGTCCTCCTCTTCTTTTTTGCTTATTGTTTGCTTGA FFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFF:FF,FFFFFFF:FF:FFFFFFFFFFFFFFFF,FFFF:FFFFF,F,,FF::,::FF,:FFFF::FF:FFF:FF:::F: NM:i:6 MD:Z:17A33C0A40C1A22A9 MC:Z:151M AS:i:98 XS:i:57 A00126:224:H7HMWDSX3:3:1112:9896:22106 145 Test_seq 170285 60 151M = 170144 -292 ATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:139C11 MC:Z:23S128M AS:i:146 XS:i:53 A00126:224:H7HMWDSX3:3:1112:14760:23954 97 Test_seq 168773 60 15S15M1D121M = 168967 345 TATACACCACAGTTAGATCAGGGTGAATTATTTTTTGGTGTATTGTTGGATTCTATTGGCTAGAATTGTGTTGAGGATTTTTGCATCTATGTTCATGAAGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTGGT F:F,FFFF,FFFFF:FFFFFFFF:FFF,FFF:::::FF:FF:,FFFFFFFFFFF,FFFFF::FFF,:,:FFFFFFFFFF,FFFFFFFFFFF,FFF:FF:FFFFFF,FFFFFFF:FFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFF,FFFF NM:i:5 MD:Z:15^T7A3G25T30G52 MC:Z:151M AS:i:109 XS:i:62 A00126:224:H7HMWDSX3:3:1112:14760:23954 145 Test_seq 168967 60 151M = 168773 -345 CTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAATGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTT FFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFF:F::FFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF: NM:i:1 MD:Z:63G87 MC:Z:15S15M1D121M AS:i:146 XS:i:67 A00126:224:H7HMWDSX3:3:1112:2302:24424 113 Test_seq 168466 60 151M = 212668 44185 GGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTT FFFFFFFFFFF::FFFFFFFFFFF:FFFF,FF:,F:FF::FFFFF,::FFFF,FFFF:FFF:::FFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF,:FFFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFF NM:i:1 MD:Z:29T121 MC:Z:133M18S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1112:2302:24424 177 Test_seq 212668 27 133M18S = 168466 -44185 TATCACTATTTGCATATGACATGATTATATACACAGAAAACCCTAAGGAATCATCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTAGTAAAGAACAACTAGAT FFFFF,FF:FFF:F,FFFF,FFFFFFFFFFFFF:FFFFFFF,,FFF,,FFF:,FFFFFFFFF:FFFF:FFFF:F:,F:FFFFFFFFF:FF:FFF,,F,F:F:FFFFFFFFFFFFFF:FFFFFF::,FFF,FFFFFF,,FFFF,:F,:::FF NM:i:7 MD:Z:4T9G10C0T17T7C53G26 MC:Z:151M AS:i:98 XS:i:83 XA:Z:Test_seq,+168238,18S133M,10; A00126:224:H7HMWDSX3:3:1112:28592:24580 97 Test_seq 169132 54 151M = 169281 300 TGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAAGAATTTATCCATTTCTTCTAAGTTTTCAAATTAGTTAGAGTACAATTTTTCGTAGTAATCTGAAATGATTCTTTTAATTTCATTTGGTTCTGTTTTGATGTGGTCCTTCTCGTTTCTTA FFFFFFFFFFF,:FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF NM:i:9 MD:Z:29G7C14G11T4C11A37C6G9T14 MC:Z:151M AS:i:106 XS:i:74 A00126:224:H7HMWDSX3:3:1112:28592:24580 145 Test_seq 169281 60 151M = 169132 -300 TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTA FF:FFFFF:FFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:28T122 MC:Z:151M AS:i:146 XS:i:103 A00126:224:H7HMWDSX3:3:1112:30861:24721 81 Test_seq 341935 60 135M16S = 341770 -300 CTGGCCCTGCCCACATCCCTAGAAGTCTTCCTTCTGCAACTCTGAAGTGGCTCTAGAGTTCTCCAATGCCCAGATAGGCAGAGCCAGACGCAGCTGGGAGTTGGAGCTAGGAGAAGAGGCAGACAAACGAGGAGTAGTCCTTAAATTCTAT FFFF:FFFFF::F:FFF::FFFF,FFFFFFF:FF:::FFF,FFFF,FFFF,F,F,FF:F::FFFFFF::F:,:FF:,FF:FFFFF::F:::F:FF,F:F:FF,:FF:F::FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF::FF:FF NM:i:1 MD:Z:40A94 MC:Z:151M AS:i:130 XS:i:0 A00126:224:H7HMWDSX3:3:1112:30861:24721 161 Test_seq 341770 60 151M = 341935 300 GCTGACAGGCTGTCCCTCCCAGGGTTGGGGTTTCCTGTTTGAAAGTGTTTAGTGTGTGCAGCCCGCTTCCAGCGTCTACTCATCTACCTTTCTGATGTAGAAGAGGGAGGGCGCCGGCAGCCTTTTTATGTCGGTAGACTTGCCCGGGGCT :FFFFF:FFFF:FFF:F:FFF:FFF:FFFFFFFFFF:F,FFFF:FF:F:FFFFFFF:FFFFFFFFFFFFFFFFF:FF:FFFF:FFFFF,FF::FF,FFFFFF:FF,FFFFFFFFFFFFFFFFF:FFFFFFFFFF,FFFF:FFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:135M16S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1112:30120:25598 81 Test_seq 170779 60 151M = 170533 -397 CCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTTAATTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:1 MD:Z:61C89 MC:Z:61M12I78M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1112:30120:25598 161 Test_seq 170533 60 61M12I78M = 170779 397 TAGTAGCTTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTTTCTTGAGTTATTGAATTGATATTCCTTTTTGTGGTTAACTTATTATCTACCCCTAATTTTCTAAGTAAAAACCT FFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:F,F,FFFF:,F,:FF:FFFF,FFFF,FF:F,::F:FFFFF:FFF,FFFF:F,FFFF,F:FFFFFF:F,:FFF:F,FFFF:FFF,FFFFFF,:, NM:i:18 MD:Z:6A39T29G25C8T8T18 MC:Z:151M AS:i:91 XS:i:0 A00126:224:H7HMWDSX3:3:1112:14570:25755 65 Test_seq 40993 2 89M = 167626 126634 ATATTTGACAAAGGCCCAGTGTCAGTTAATTGGGGAAAAGATAGTCTTTTTAACAAATGGTGCTGGCATAACTGCATATCCACTTGCAA FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFF,F NM:i:5 MD:Z:14A23T35G7T0C5 MC:Z:89M AS:i:67 XS:i:64 XA:Z:Test_seq,+213324,89M,5;Test_seq,-167626,89M,5; A00126:224:H7HMWDSX3:3:1112:14570:25755 129 Test_seq 167626 0 89M = 40993 -126634 TCGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATAT FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFF NM:i:1 MD:Z:1T87 MC:Z:89M AS:i:87 XS:i:87 XA:Z:Test_seq,-213324,89M,1;Test_seq,-40993,89M,3; A00126:224:H7HMWDSX3:3:1112:5891:26256 65 Test_seq 404397 21 31M120S = 214069 -190329 TTCTGCTCTGTCCTATAGGGTCACTATGAGTGATCTTATAATAGTGGTCTTATACAATATTTGTCCTTTTGCCTCTGACTAATTTCACTCAGCATAATGCCTTCCAGGTTCCTCCATGTTATGAAATGTTTCACAGATTCGTCACTGTTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:31 MC:Z:151M AS:i:31 XS:i:26 SA:Z:Test_seq,214275,-,110M1I9M31S,12,2; A00126:224:H7HMWDSX3:3:1112:5891:26256 2129 Test_seq 214275 12 110M1I9M31H = 214069 -325 AGAACAGTGACGAATCTGTGAAACATTTCATAACATGGAGGAACCTGGAAGGCATTATGCTGAGTGAAATTAGTCAGAGGCAAAAGGACAAATATTGTATAAGACCACTATTATAAGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:94C24 MC:Z:151M AS:i:107 XS:i:100 SA:Z:Test_seq,404397,+,31M120S,21,0; XA:Z:Test_seq,+166640,31S120M,4;Test_seq,+400061,31S120M,5;Test_seq,+48610,36S115M,5; A00126:224:H7HMWDSX3:3:1112:5891:26256 129 Test_seq 214069 60 151M = 404397 190329 ACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAACGGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:146T4 MC:Z:31M120S AS:i:146 XS:i:103 A00126:224:H7HMWDSX3:3:1112:32380:26944 81 Test_seq 9289 60 151M = 9121 -319 TCTGCACTCGGTTGTACCACCTTTCTTGGAAATAGGCATACATATGGATCTCTTCCAGTCAGTTGGCTAGGTAGCTGACTTCCAAATGTCTTGGCACAGACCGGTGAGCACTTTCAGTGCTGCATCTATTTTTCTCAATTGGTATTCTCTC FF,::FFFF:F,F:FFFF::FF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,:F:FF:F:FFFFF:F:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:F:FFFF:,FFFF,FFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1112:32380:26944 161 Test_seq 9121 60 151M = 9289 319 GATCCATCCGACATCAGCAAAGATATCCCGTGTTCCACCCCTTCTTCAGAATCTGGCTTGAATTCCTGGCAGTTCCCTGTCAAAATACTGCTGCAGCCACTTTTAAATGACCTTCAGCAAAATTTTACTTGGCAGTGGGTTATTTTATATT FFFFFFF::F:FF:F:FF,FFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFF,,F:,FFFF:FFFFFF,FFF:FF:FFFFFFFF,FFFFF,FFFF,FF,F NM:i:1 MD:Z:141T9 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1112:25012:28494 81 Test_seq 339924 60 126M25S = 339682 -368 CACCCTTTGGAGGACATTCACATCAGCATTTATTTTAAACTGTTACAGCAACTCAGAAAAACAGGTTGGAAAATACAGAAACTTTCCATGAAAAATGACTAAAAGGAAGTCAATGTAAGAGTTTTATAAGAAAATAAAATTCAAACCGCAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:126 MC:Z:151M AS:i:126 XS:i:0 A00126:224:H7HMWDSX3:3:1112:25012:28494 161 Test_seq 339682 60 151M = 339924 368 TCAGAGCTTGGAACCAAAAACCAAACCCACTGGCTTCCAGTCGATTCTGACTCATAGCGACCCTATAGGACAGAGTAGAACTGCCCCATAGAGTTTCCAAGGAGCGCCTGGTGGATTTGAACTGCCAACCTCTTGGTTAGCAGCCATAGCA FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:126M25S AS:i:151 XS:i:89 A00126:224:H7HMWDSX3:3:1112:27941:31688 65 Test_seq 213182 7 151M = 167567 -45616 AGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCAGCTGATATTTGAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:130A20 MC:Z:19S132M AS:i:146 XS:i:141 XA:Z:Test_seq,-167706,151M,2; A00126:224:H7HMWDSX3:3:1112:27941:31688 129 Test_seq 167567 26 19S132M = 213182 45616 GAGGCTGGGCGGTGGGGAGTGATCCATTTGGAGTTAGTTTTTGTGCATAGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFF NM:i:2 MD:Z:7C21G102 MC:Z:151M AS:i:122 XS:i:110 XA:Z:Test_seq,-213340,120M31S,2; A00126:224:H7HMWDSX3:3:1112:19931:32690 97 Test_seq 168171 7 151M = 168471 451 GGGGGCTACTGTGAATGGTATTGATTTAGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGAT FFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:27G72T10T39 MC:Z:151M AS:i:136 XS:i:131 XA:Z:Test_seq,-212717,151M,4; A00126:224:H7HMWDSX3:3:1112:19931:32690 145 Test_seq 168471 60 151M = 168171 -451 TAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATC FFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:91 A00126:224:H7HMWDSX3:3:1112:24551:34053 97 Test_seq 200001 60 151M = 200346 496 GATCAAATGTTTTTGGAAACATTTTCATTATGAGCAGAGGTTTTTTTTGTTTGTTTTTGTTTTTTAATTTCAGCAATCCAGGGTTCAGATTGCTCTACTATTTGCTAACTTTAGGAAGTGTAAAAAGCATATTTTTCTTACCTGAAAAGGA FFFFF:::FFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFF::FF:FFFFF:::FFFFFFFFFF:FFF:FFFFFFF::FFFFF,FFFFFF,FFFFFFFF:FFFFFFFF:,:F,F:,F:F NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1112:24551:34053 145 Test_seq 200346 60 151M = 200001 -496 TTTGAACTGCCAGTCTTTTGGTTAGCAGCTGTAGCACTTAACTACTACGCCACCAGGGTTACCACTCAGCACAGAGGAAAAGCTTAATAAATGGTAACTATCATAATCGTCAAGGAGCCCTGGTGGCGTGGTGGTTAAAGCACTTGGCTGT FF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1112:23285:36213 97 Test_seq 170144 60 151M = 170428 435 GATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFF:FFFFFFFFFF::FFF:,FF::::FFFFFFFFFFFFF,FFFFFFFFFFF NM:i:4 MD:Z:25A25C0A65A32 MC:Z:151M AS:i:131 XS:i:59 A00126:224:H7HMWDSX3:3:1112:23285:36213 145 Test_seq 170428 60 151M = 170144 -435 ATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCATTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:62T88 MC:Z:151M AS:i:146 XS:i:25 A00126:224:H7HMWDSX3:3:1112:27986:36777 113 Test_seq 167313 0 151M = 213805 46493 AGAGATCAGGCGCTGGTCGGAGATGTCATAGCTGAAAATTCTTTCCCAGTCTGTAAGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCGTCATTTTTG FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:10A7T17T104A9 MC:Z:151M AS:i:131 XS:i:131 XA:Z:Test_seq,+213571,151M,4;Test_seq,-400734,126M25S,4; A00126:224:H7HMWDSX3:3:1112:27986:36777 177 Test_seq 213805 44 151M = 167313 -46493 CTTCACTAAAGAAGATATTCAGGCAGCTAACAGATACATGAGAAAATGCTCTCGATCATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCATTAATCCAAAAAACACAAAATAATAAA FFFF:F:FFFFFFFFFFFFFF,FFFFFF:FFFFFFFFFFF:FFF:FFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:86G64 MC:Z:151M AS:i:146 XS:i:125 XA:Z:Test_seq,+167079,151M,6; A00126:224:H7HMWDSX3:3:1112:24695:36714 81 Test_seq 169310 60 151M = 169001 -460 TTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:17S134M AS:i:151 XS:i:110 A00126:224:H7HMWDSX3:3:1112:24695:36714 161 Test_seq 169001 60 17S134M = 169310 460 CCAAAAGCGATCTAAGCCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGTCATCTGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTGTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF:FF:FFF:FFFFFFFFFFFFFFF,FFFFFF:FFFFFFF:FFFFFFFFFF:FFFFF:FF:FFFFF,FFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF: NM:i:4 MD:Z:35C1G26A31T37 MC:Z:151M AS:i:114 XS:i:60 A00126:224:H7HMWDSX3:3:1112:22589:36886 65 Test_seq 213149 6 151M = 167532 -45618 CAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:0T150 MC:Z:28S123M AS:i:150 XS:i:146 XA:Z:Test_seq,-167739,151M,1; A00126:224:H7HMWDSX3:3:1112:22589:36886 129 Test_seq 167532 42 28S123M = 213149 45618 CCTTTGGTGTTGGTTTTGTTTTTTGATCGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCCACCTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATCCTTTTGCAAATGGATATCCAGTTATGCCAGCA FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFF:FF::F::FFFFFF,F,FF:F NM:i:3 MD:Z:10G32T46T32 MC:Z:151M AS:i:108 XS:i:88 XA:Z:Test_seq,+400995,28S118M5S,6; A00126:224:H7HMWDSX3:3:1113:12011:1908 81 Test_seq 170612 60 151M = 170321 -442 ATTGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATGTTTAGTCCCCCTTTGTGATTATTGTGATC FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:73G6A38G1A9T4T14 MC:Z:151M AS:i:121 XS:i:37 A00126:224:H7HMWDSX3:3:1113:12011:1908 161 Test_seq 170321 60 151M = 170612 442 TAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCATGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFF:FFFF,FFF:F:: NM:i:1 MD:Z:56G94 MC:Z:151M AS:i:146 XS:i:54 A00126:224:H7HMWDSX3:3:1113:24659:2049 97 Test_seq 362109 60 151M = 362364 390 ATGCGCCCACGTGCACCATGGTTTCTTCAGCCATGCTTCTTACTACAGAAAGTGATTCCCAACTCTACATACTCAGGAAAGTTATTGGGTTGTCTTTTTCTGTTTAAAACTATTTGTGCTTATCGTGGACAATATTGAAAGTAATAAGAAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:135M16S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1113:24659:2049 145 Test_seq 362364 60 135M16S = 362109 -390 ATGAATACATATCAGAGATTTATTTGACTCATTAATGAGGGAACCAGTAAGATGGTAAAGCTAGTTCCAAAACGTATAGGGAAAACAGAAGTATTTTAGTTATTAAAAGAAAGTTGAAATAAAATTTCTGGATCACTCTTAAAACCCAGAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:135 MC:Z:151M AS:i:135 XS:i:0 A00126:224:H7HMWDSX3:3:1113:13123:3364 81 Test_seq 168524 60 151M = 168222 -453 CTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGAT FFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1113:13123:3364 161 Test_seq 168222 43 151M = 168524 453 TTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGCTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF NM:i:4 MD:Z:49T37T36G18A7 MC:Z:151M AS:i:131 XS:i:109 XA:Z:Test_seq,-212673,7S144M,7; A00126:224:H7HMWDSX3:3:1113:4526:3975 113 Test_seq 168435 60 151M = 212733 44299 TAGGACTCCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:7T143 MC:Z:151M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1113:4526:3975 177 Test_seq 212733 20 151M = 168435 -44299 CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACT F:FF:FFFFFFF::FFFFFFF,:FFF:,F,FFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFF,:FFFF,F:FFFFFFFFFF:FF:FFFFFF:F,FFFFFFFFF:::FFFFF,FFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFF NM:i:3 MD:Z:41G51A10A46 MC:Z:151M AS:i:136 XS:i:126 XA:Z:Test_seq,+168155,151M,5; A00126:224:H7HMWDSX3:3:1113:5565:7560 81 Test_seq 166434 60 120M31S = 166317 -237 AAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCTAAAATTCAAGTCATATCTTGAAATTCACTA :FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:120 MC:Z:151M AS:i:120 XS:i:30 A00126:224:H7HMWDSX3:3:1113:5565:7560 161 Test_seq 166317 60 151M = 166434 237 GCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTCCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFF:FFFF NM:i:1 MD:Z:49G101 MC:Z:120M31S AS:i:146 XS:i:32 A00126:224:H7HMWDSX3:3:1113:12644:7701 97 Test_seq 424202 60 151M = 424407 339 TGGGGCAGAAGACTGCTGTTTTTCACACTTAGAGAATAAGACCTTTTCAAACTCAACATGTCCACTTGGATGTCTAATAGACATCTTAAACTTAATATATTTAAAACAATATTTGGCCCCAAAGCTGCCCTATTTTGTGTCAGCTGATGGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:134M17S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1113:12644:7701 145 Test_seq 424407 60 134M17S = 424202 -339 ATATCCAATCCTTCAGGAAATCCTTTAGAATATCTCCAGACTCTGACCATCTTGCGCTTCCTCTGCTGTCACCATCCTGGGTCAAGCCCATCATCTCTCACCTGAACTCCTGCTCATAATCTCCTCCCAGATCTAAACTACACAGTATCTA FF:FFFFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FF:FFFFFFFFF NM:i:0 MD:Z:134 MC:Z:151M AS:i:134 XS:i:0 A00126:224:H7HMWDSX3:3:1113:13765:8735 65 Test_seq 328153 19 2S25M124S = 167568 -160586 AAGGAGCCCTGGTGGTGCAGTGGTTAGATCCAAATTCCAATGTCATATTTTAAGGGGATAGAGAAACAAATCACTAATTTCATATGGAAGGGAAAGAACCCCCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCA FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF,FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF NM:i:0 MD:Z:25 MC:Z:151M AS:i:25 XS:i:21 SA:Z:Test_seq,167849,-,124M27S,60,1; XA:Z:Test_seq,+141607,26M125S,1;Test_seq,+432045,26M125S,1; A00126:224:H7HMWDSX3:3:1113:13765:8735 2129 Test_seq 167849 60 124M27H = 167568 -405 TGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGAATTTGGAT FFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF,FFFFFFFF:FFFFFFFFFFF NM:i:1 MD:Z:115T8 MC:Z:151M AS:i:119 XS:i:75 SA:Z:Test_seq,328153,+,2S25M124S,19,0; A00126:224:H7HMWDSX3:3:1113:13765:8735 129 Test_seq 167568 32 151M = 328153 160586 GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGC FFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:2S25M124S AS:i:151 XS:i:136 XA:Z:Test_seq,-213320,140M2I9M,3; A00126:224:H7HMWDSX3:3:1113:8892:9878 81 Test_seq 396946 60 151M = 396701 -396 CATCTGATGGTCTATTCTGCAGTCGGCTCCTGGCCTTGTTCTGACTGATGATATTGAGCTTCTTCATTGTCTCTTTCCACAGATGTAGTTGATTCGATTCCTGTGTATTCTATCTGGTGAGGTGTGTATAGTCACAGTTTATTTTGTTGAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:95A55 MC:Z:25S126M AS:i:146 XS:i:58 A00126:224:H7HMWDSX3:3:1113:8892:9878 161 Test_seq 396701 60 25S126M = 396946 396 TTTTTTCTAGTATACCATTTTGATCAGTCTCTCCTGACATCCATTTTGATGTTTCTTTCCTGTCTTTTTAATGACCTTTTGCTTTGTTCATGTATGAGGTCCCTGATTTCATCCCACAGCTCATCTGGTCTTTGGTCATTAGTGTTCAGTG FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,::FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:126 MC:Z:151M AS:i:126 XS:i:0 A00126:224:H7HMWDSX3:3:1113:7084:10441 81 Test_seq 167490 60 151M = 167316 -325 CTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTACATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATC FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF NM:i:1 MD:Z:102G48 MC:Z:21S130M AS:i:146 XS:i:96 A00126:224:H7HMWDSX3:3:1113:7084:10441 161 Test_seq 167316 7 21S130M = 167490 325 ATCTGACACATTTAAGTGATCGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFF:FFF NM:i:3 MD:Z:7A44A73T3 MC:Z:151M AS:i:116 XS:i:111 XA:Z:Test_seq,-213589,130M21S,4;Test_seq,+400737,21S123M7S,5; A00126:224:H7HMWDSX3:3:1113:3025:10614 81 Test_seq 386034 60 151M = 385792 -393 CGTTTCTTCTGTTTTTGCTCCTCATCTTCTTGCCTATTTGAGACATCACAATTTGTTTGTGCAATCCTGGAAATTCTCCTTGTTTCCACCCTCAACCCATCTCAGCCTGCTGCCTTCTTGTAGACCAGGGTCCCTGACTCTGGCCCATGCA :FFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FFF:FFFFFFFFFFFFFFFFFFFFFFF:::FFF:FFFFF:FFF:FFFFFF,FFFF,FFF:FFFF::F:FF:F:FFFFFFFFF NM:i:0 MD:Z:151 MC:Z:15S136M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1113:3025:10614 161 Test_seq 385792 60 15S136M = 386034 393 GCCAGAAGGTTCCTTAATCCCTATCCCAAGGGATTTATCTCTAAAAAGAAATGCTGTTTTGTGCGTGGAATTCAGTCTGACTCATAGCACCCTGTATGACGAAGTAGAACTGCCCCACACGGTTTCCTAGGCTGTGATATTTACAAAAAAA FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FF:FF:FFFF,FFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:,FFFFFFFFFF:FFFFFFF,FFF NM:i:0 MD:Z:136 MC:Z:151M AS:i:136 XS:i:0 A00126:224:H7HMWDSX3:3:1113:6903:10942 81 Test_seq 166434 60 120M31S = 166317 -237 AAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCTAAAATTCAAGTCATATCTTGAAATTCACTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:0 MD:Z:120 MC:Z:151M AS:i:120 XS:i:30 A00126:224:H7HMWDSX3:3:1113:6903:10942 161 Test_seq 166317 60 151M = 166434 237 GCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTCCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:1 MD:Z:49G101 MC:Z:120M31S AS:i:146 XS:i:32 A00126:224:H7HMWDSX3:3:1113:32081:11365 97 Test_seq 168282 60 151M = 168457 326 GCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCCTGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTG FFFFFFFFF:FFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFF,F,FFF:F:F:F:FFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:0T54A7G0G18A67 MC:Z:151M AS:i:130 XS:i:92 A00126:224:H7HMWDSX3:3:1113:32081:11365 145 Test_seq 168457 60 151M = 168282 -326 AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGG FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFF NM:i:1 MD:Z:38T112 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1113:11822:11381 81 Test_seq 168651 60 127M24S = 168421 -357 CTTTTCTGCATCAATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCGTACCTGGTATAAATCCCACTTGATCACTCTGTTCGGGCCTCTTCTTGCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF NM:i:3 MD:Z:12T52T34A26 MC:Z:151M AS:i:112 XS:i:51 A00126:224:H7HMWDSX3:3:1113:11822:11381 161 Test_seq 168421 60 151M = 168651 357 CTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:127M24S AS:i:151 XS:i:69 A00126:224:H7HMWDSX3:3:1113:30861:11913 97 Test_seq 168282 60 151M = 168457 326 GCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCCTGTATACAATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTG FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FF:FFFFFF NM:i:5 MD:Z:0T54A7G0G18A67 MC:Z:151M AS:i:130 XS:i:92 A00126:224:H7HMWDSX3:3:1113:30861:11913 145 Test_seq 168457 60 151M = 168282 -326 AATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGG FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:38T112 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1113:25021:12258 81 Test_seq 384484 10 51M = 204882 -179653 GATAAACGGAGAAAAAATTGAAGTTGTCAAGGATTTCATTTTACTTGGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:15G35 MC:Z:51M AS:i:46 XS:i:42 XA:Z:Test_seq,-449828,47M4S,1;Test_seq,+136952,51M,3; A00126:224:H7HMWDSX3:3:1113:25021:12258 161 Test_seq 204882 0 51M = 384484 179653 GATAAACAGAGAAAAGATTGAAGTTGTCAAGGATTTCATTTGACTTGGATC FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:2 MD:Z:16T24T9 MC:Z:51M AS:i:41 XS:i:41 XA:Z:Test_seq,+384484,51M,2;Test_seq,+449828,47M4S,2;Test_seq,-333028,51M,4; A00126:224:H7HMWDSX3:3:1113:30337:12696 97 Test_seq 169850 60 151M = 170199 500 TTCTAGAGAATGTTCCATGTGCACTAGAAAAAAAAGTATACTTTGCAGCAGTTGGGTGGAGAGTTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTCCTGTC FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:16C23T35T47G26 MC:Z:151M AS:i:131 XS:i:61 A00126:224:H7HMWDSX3:3:1113:30337:12696 145 Test_seq 170199 60 151M = 169850 -500 TTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATA FFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:63A87 MC:Z:151M AS:i:146 XS:i:75 A00126:224:H7HMWDSX3:3:1113:14434:14841 81 Test_seq 170206 60 151M = 170093 -264 CTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGAT ,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF: NM:i:1 MD:Z:56A94 MC:Z:151M AS:i:146 XS:i:88 A00126:224:H7HMWDSX3:3:1113:14434:14841 161 Test_seq 170093 47 151M = 170206 264 TTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F:FFFFFFF:FFF,FFF::, NM:i:5 MD:Z:14C7G20A58C0A47 MC:Z:151M AS:i:126 XS:i:101 XA:Z:Test_seq,-38489,151M,10; A00126:224:H7HMWDSX3:3:1113:14290:15405 97 Test_seq 168462 60 151M = 168688 349 GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFF:FF::,:FFFFF:FFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF:FFF NM:i:1 MD:Z:33T117 MC:Z:123M28S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1113:14290:15405 145 Test_seq 168688 60 123M28S = 168462 -349 TCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTTAAACAGATGGGAAAACCAAGGCCCAGA FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:28T94 MC:Z:151M AS:i:118 XS:i:55 A00126:224:H7HMWDSX3:3:1113:31087:16000 81 Test_seq 167526 52 151M = 167317 -360 TTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATCA FFFFF:FFFFFFF:FFFFFF:FFFFFF:FFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:1 MD:Z:118T32 MC:Z:18S133M AS:i:146 XS:i:121 XA:Z:Test_seq,+213362,98M2I23M2I26M,6; A00126:224:H7HMWDSX3:3:1113:31087:16000 161 Test_seq 167317 7 18S133M = 167526 360 TGCTCTCTTTCTCCCTCAATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTAGTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCT FFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFFFFFFF:F,FFFFFFF:FFF:F:FFFFFFFF::FFFFFFF::,FFFFFF:FFFF:FFFF:FFFF:F,FFFF,FFFF:F:FFFFFFFFFFFFFF:,F,F,FF:FFFF NM:i:3 MD:Z:6A44A12C68 MC:Z:151M AS:i:118 XS:i:113 XA:Z:Test_seq,-213585,133M18S,4; A00126:224:H7HMWDSX3:3:1113:14027:16297 97 Test_seq 168274 27 151M = 168525 402 GAGACTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTACACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAA FFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFF,FFFFF NM:i:6 MD:Z:8T54A4T3G18A28T30 MC:Z:151M AS:i:121 XS:i:106 XA:Z:Test_seq,-212614,151M,9; A00126:224:H7HMWDSX3:3:1113:14027:16297 145 Test_seq 168525 60 151M = 168274 -402 TCCATTTAGAGTGATATTGGCTGTTCGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATC FFF:FF,FFFFFFFFFF,FFFFFFFFF:F,FFFFFFF:F:FFFFFFF::F:FF:FFFFFFFF:FFF:FFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:25G125 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1113:10493:17127 81 Test_seq 167169 52 151M = 166938 -382 TAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:74T76 MC:Z:151M AS:i:146 XS:i:121 XA:Z:Test_seq,+213715,151M,6; A00126:224:H7HMWDSX3:3:1113:10493:17127 161 Test_seq 166938 7 151M = 167169 382 GAGTGGGATTTCTGGGTTGTATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATAGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCCGCAGCCTCTCCAACATTTATTATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFF:FF:FFFFFFFFFF:FFFFFF:FFF:FFFFF:FFF,FFFF NM:i:4 MD:Z:60G29A27G7A24 MC:Z:151M AS:i:131 XS:i:126 XA:Z:Test_seq,-213946,151M,5; A00126:224:H7HMWDSX3:3:1113:29857:18380 97 Test_seq 248238 60 37S114M = 248425 338 GTCCACTGGCTCATGGAGCCCCTATGGACCTGGGATCTAAATGCTTTGCTACATGTACATGGAAACCCTGGTGGCGCAGTGGTTAAGAGCTACGTCTGCTAACCAAAAGGTCAGCAGTTCGAATCCCCCAGGTGCTTCCTTGGAAACTCTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF NM:i:0 MD:Z:114 MC:Z:151M AS:i:114 XS:i:39 A00126:224:H7HMWDSX3:3:1113:29857:18380 145 Test_seq 248425 60 151M = 248238 -338 TTTATATGTACAAAGCACAAGCTTTGGATTGAGATGGTTCCGAGTTCAAATTTCTTTACTAAATTTATGGTCTTAGGAAAGTTATTTAACATTTCTAAGGTTCAGTTTTCTCATTTATAAAATGATAATGATAATAGCTGTCTGTAGGCTT FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:37S114M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1113:4444:18584 113 Test_seq 213611 0 126M25S = 167529 -46058 AAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGGGAAAGAATTTTCAGCTATGACATCTCAGACCAGCGCCTGATCTCTAAAATCTACATGATTTAGATGAATGGTTGCTAGCATTTAA F:FF:FFFFF,FF,::FFFFFFFFFFF:FFFFFFFFFF:FFF,:F:F:FFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:58T33C26T6 MC:Z:151M AS:i:111 XS:i:111 XA:Z:Test_seq,+167298,25S126M,3;Test_seq,+400719,25S126M,5; A00126:224:H7HMWDSX3:3:1113:4444:18584 177 Test_seq 167529 53 151M = 213611 46058 CATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTT FFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:147A3 MC:Z:126M25S AS:i:147 XS:i:122 XA:Z:Test_seq,+213359,101M2I23M2I23M,6; A00126:224:H7HMWDSX3:3:1113:25536:18818 97 Test_seq 170292 60 151M = 170625 471 GAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGTTCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAA FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:63A87 MC:Z:13S138M AS:i:146 XS:i:76 A00126:224:H7HMWDSX3:3:1113:25536:18818 145 Test_seq 170625 60 13S138M = 170292 -471 GTTGTTATACCTCTTTGTGGTTACCTGAATATTTACCCCTATTTTTCTAAGTAAAAACCTAACTCGTATTGCTCTATATCGCCTTGTATCACTCTCCATATGGCAGTTCTATGCCACCTGTATTTAGTCCCTCTTTTTGATTATTGTGATC FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:10 MD:Z:13T1T35T4C1T1G6A18C9A5T35 MC:Z:151M AS:i:88 XS:i:30 A00126:224:H7HMWDSX3:3:1113:26829:18897 81 Test_seq 168461 60 151M = 168270 -342 AGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAG FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:34T116 MC:Z:151M AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1113:26829:18897 161 Test_seq 168270 60 151M = 168461 342 ACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCTGGGTACCCTTTATTTCTTTGTCTAGC FFFFFFFFFFFFFFFFFFFFFFFF:F,:FF:FFFFFFFFF,FFFFFFFFF,:FFFFFFFFFFFFFFF,:FFFFFFFF,FF:FFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFF:F,FFFFFFFFFFFFFF NM:i:4 MD:Z:1T74G18A31A23 MC:Z:151M AS:i:134 XS:i:91 A00126:224:H7HMWDSX3:3:1113:14055:19225 97 Test_seq 171575 60 14S137M = 171741 317 TTGCCAATTGACTTAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTATTAATTTTTCCACTATGTTCTTGAATAATCTTTTT FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFF:F:FF NM:i:4 MD:Z:8T23T83C13C6 MC:Z:151M AS:i:117 XS:i:78 A00126:224:H7HMWDSX3:3:1113:14055:19225 145 Test_seq 171741 60 151M = 171575 -317 CCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGC :FFFFFFFF::FFFFFFF:FF,FFFFFFFFF,F:FFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:14S137M AS:i:151 XS:i:25 A00126:224:H7HMWDSX3:3:1113:15085:20322 97 Test_seq 169286 60 151M = 169619 461 GGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTT FFF,FF,,FFFFFF:FFFFF,F::F,FFF:F:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,:FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFF:FF,F:FFF:FF:FFFF,F:FFFFFF::: NM:i:1 MD:Z:23T127 MC:Z:128M23S AS:i:146 XS:i:104 A00126:224:H7HMWDSX3:3:1113:15085:20322 145 Test_seq 169619 60 128M23S = 169286 -461 TTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCGTTTCTTTCCATG FFF,FF:FFFFF::FFFFF:FF:FF::FF:FFFF:FFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFF:FFFF:FF NM:i:4 MD:Z:9T11A22G70A12 MC:Z:151M AS:i:108 XS:i:0 A00126:224:H7HMWDSX3:3:1113:5945:21277 97 Test_seq 168437 60 151M = 168659 373 GGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTT FF:FFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:F,FFFFFFFFFFFFFFFF,FFFFFFFFFF,FFF NM:i:1 MD:Z:58T92 MC:Z:151M AS:i:146 XS:i:85 A00126:224:H7HMWDSX3:3:1113:5945:21277 145 Test_seq 168659 60 151M = 168437 -373 CATCAATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGAT :F:F:FFFFFFFFFFF,FFF::F:FFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFF:FF,FFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFFFFFF NM:i:2 MD:Z:4T52T93 MC:Z:151M AS:i:141 XS:i:55 A00126:224:H7HMWDSX3:3:1113:8675:22780 97 Test_seq 168347 60 24S127M = 168519 323 CATGAGAGTAAAGAAAATGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTACTTCTCTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAGTAAGAGCGGTGATAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF NM:i:5 MD:Z:18A21G19T4T45A15 MC:Z:151M AS:i:102 XS:i:46 A00126:224:H7HMWDSX3:3:1113:8675:22780 145 Test_seq 168519 60 151M = 168347 -323 GTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTACATCTATTGAT FFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:139G11 MC:Z:24S127M AS:i:146 XS:i:84 A00126:224:H7HMWDSX3:3:1113:5159:23359 97 Test_seq 238719 60 21S130M = 238959 391 TAGTCCAATCCCTATTTGATCGATCAAAAGATATTGACCTCCTAGGGTTTTTAGCATGTATTTTCAAATCATCCTAAAGGAAAGTAATACAAACTTATAGTCTCACCTGTTGTGTGAAAAGACCTGTTTTTCAAGATGCTTGTCTACACAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF NM:i:0 MD:Z:130 MC:Z:151M AS:i:130 XS:i:0 A00126:224:H7HMWDSX3:3:1113:5159:23359 145 Test_seq 238959 60 151M = 238719 -391 GGTCTTTGTCCATTGTTCTACTGATATTAGTCTCTTTTAGTTGATTCACAGGACTTGAGAGGTTATTGCTATTAATCTCTATCACATATTTTGCAAATATATTCCTCAGTTTCTTTACTTGCCTTTTATTTATTTTTTCATTTTAAGGTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF NM:i:0 MD:Z:151 MC:Z:21S130M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1113:32551:24267 97 Test_seq 258024 60 16S135M = 258130 257 CTCCTTCAAACTGATCGATCACCAACTTCATATGGAAAGGGAAGCATCTCTGGATAAGTAAATCATTACTGAAAAAGAAGAACAAAGTGAAAGGCCTCACACTGTCTGATTTTAGAACCTATTATAATGCCACAGTAGTCAAAACAGCTTG FFFFFFFF:FFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:135 MC:Z:151M AS:i:135 XS:i:32 A00126:224:H7HMWDSX3:3:1113:32551:24267 145 Test_seq 258130 60 151M = 258024 -257 TATAATGCCACAGTAGTCAAAACAGCTTGGTACTGTTACAGCAACAGATACATAGACCAATGGAACAGAATTGAGAATCCAGACATAAATTCGTCCACTTAAGAGCAGCTGATATTTGACAAAGGCCCAAAGTCCATTAATTGGGGAAAAG FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:16S135M AS:i:151 XS:i:51 A00126:224:H7HMWDSX3:3:1113:27046:26068 97 Test_seq 168498 60 151M = 168543 178 TCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:138A12 MC:Z:133M18S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1113:27046:26068 145 Test_seq 168543 60 133M18S = 168498 -178 GGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCGATCCCAGTGGTCATGGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:93A26T12 MC:Z:151M AS:i:123 XS:i:75 A00126:224:H7HMWDSX3:3:1113:24804:26537 97 Test_seq 170240 60 151M = 170399 310 CTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCT FFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFF,F:FFFFFFF,FFFFFFFF,FFFFFFF,F::F:F::FF:::FFFFFF,FFF NM:i:1 MD:Z:22A128 MC:Z:151M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1113:24804:26537 145 Test_seq 170399 60 151M = 170240 -310 GTGCATTTAGTCCATTTACATTCAGTGTAATTATAGCTAAATAAGTTTTTAGTGTTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCAATTTCATTTTTCCACATACTTTTTTGTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATC FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:25C10A17C35C0T32A14G11 MC:Z:151M AS:i:116 XS:i:29 A00126:224:H7HMWDSX3:3:1113:21576:28995 81 Test_seq 221298 60 151M = 221041 -408 CTGTGATTGATTTATTTCACTCAGCATAATGCCCTCCAGATTCATCTATGTTGTGAGCTGCTTTGCAGATTCGTCATTGTTCTTTATCGTCACATAGTACTCCATTGTGTGTATGTACCACAATTTGTTTATCCATTCATCTGTTGATAGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:42 A00126:224:H7HMWDSX3:3:1113:21576:28995 161 Test_seq 221041 60 151M = 221298 408 TCAGCCCAGTAATTTGGTCCCTCAGGGAGTTTAGATGTGTCTGTGGACCTTCCATGACCTTGCCTTGTATAAGCTTTTCTGGCTTCCCCAGTATTGTGGACTGTCTTACCCTTCACCTAAGTTACCATTTATCTATTGTCTAGTGTTTTTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:43 A00126:224:H7HMWDSX3:3:1113:32172:31751 97 Test_seq 167149 42 151M = 167384 367 ATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FF:F:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,F:FFFFF:FFF:FFFFFFFF,FF,F:,:FFF,FF:FFF:FF:::,FFFFFFFFF,:FFFFFFFF: NM:i:1 MD:Z:94T56 MC:Z:132M19S AS:i:146 XS:i:126 XA:Z:Test_seq,-213735,151M,5; A00126:224:H7HMWDSX3:3:1113:32172:31751 145 Test_seq 167384 31 132M19S = 167149 -367 TTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTGATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCACTCAGATAAGAGAAGGTC F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFF,FFFFFF:FF:FF:,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF::FFFF:FFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFF NM:i:1 MD:Z:54T77 MC:Z:151M AS:i:127 XS:i:113 XA:Z:Test_seq,+213528,28S123M,2; A00126:224:H7HMWDSX3:3:1113:25174:32252 97 Test_seq 167567 32 14S137M = 167823 407 TATGGGATGTTGAGTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCGATTGACTGACACTGGTCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:2 MD:Z:108C8A19 MC:Z:151M AS:i:127 XS:i:112 XA:Z:Test_seq,-213335,125M2I10M14S,5; A00126:224:H7HMWDSX3:3:1113:25174:32252 145 Test_seq 167823 60 151M = 167567 -407 ATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATC FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:150T0 MC:Z:14S137M AS:i:150 XS:i:96 A00126:224:H7HMWDSX3:3:1113:29921:33176 81 Test_seq 74215 60 151M = 74097 -269 GAAGGATCATAGGAAAGGTATAGGTTACTGCTGGAAGGAGCATCTTAACAAGAGTTGGGAGTCCAGGGAAGCCTTCTGGAGACCAAATCTGTTACCATCCGACTCATAGTGACCCTATAGGACAGAATAGAACTCCCCCATAGGGTTTCCA FFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF,FF:FFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:27S124M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1113:29921:33176 161 Test_seq 74097 60 27S124M = 74215 269 CCTGATGGCACAGTGGCTTAGCTCTCAACTCTCTCCTAGGCACTGATGATTGAGTAATGAACACTCTAGACATGTTCCCTGTCTTTACTTACAATCTATTGGGGAAATGGACATCTAATCAAGCAGTTATAACACAACATGCTATGAAGGA FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F:FFFF,F:FFFFF:F:FFF:FFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFF:FFFFFFFFFFFFF NM:i:0 MD:Z:124 MC:Z:151M AS:i:124 XS:i:0 A00126:224:H7HMWDSX3:3:1113:23393:35117 97 Test_seq 170266 39 60M = 170266 60 GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGG FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:60 MC:Z:60M AS:i:60 XS:i:48 A00126:224:H7HMWDSX3:3:1113:23393:35117 145 Test_seq 170266 39 60M = 170266 -60 GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:60 MC:Z:60M AS:i:60 XS:i:48 A00126:224:H7HMWDSX3:3:1113:23086:35149 97 Test_seq 170266 39 60M = 170266 60 GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:60 MC:Z:60M AS:i:60 XS:i:48 A00126:224:H7HMWDSX3:3:1113:23086:35149 145 Test_seq 170266 39 60M = 170266 -60 GCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGG FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:60 MC:Z:60M AS:i:60 XS:i:48 A00126:224:H7HMWDSX3:3:1113:25057:36714 81 Test_seq 168407 60 151M = 168238 -320 TTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGC ,FFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:88T62 MC:Z:15S136M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1113:25057:36714 161 Test_seq 168238 24 15S136M = 168407 320 CCCCAAGGTGTATTTAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGACACTCTGCCAAACTCTTCTATTAGTGTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAGTGATAGCT FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:F::FFFFFF:FFFFFFFFFFF:FFFFFFFF:FFFFFFFFF:F,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:33T4G5T18T44G18A8 MC:Z:151M AS:i:106 XS:i:93 XA:Z:Test_seq,-212673,8S128M15S,7; A00126:224:H7HMWDSX3:3:1114:11785:1485 97 Test_seq 170257 60 151M = 170566 448 TTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTA FFFFFFFF:FFFFFFFFFFF:F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFF:F:FFFFFFFF:FFFFFFFFF,F:FFF,FFF:FFFFFFFF:FF:F:FFF:FFFFFFFFFFF:FFFFFFFFFF,FF:FFF,F NM:i:1 MD:Z:5A145 MC:Z:32M12I107M AS:i:146 XS:i:80 A00126:224:H7HMWDSX3:3:1114:11785:1485 145 Test_seq 170566 60 32M12I107M = 170257 -448 TTGACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATGGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATGACT FFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFF:FFF::F,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:20 MD:Z:2A10T32A3A3T64G6A8C3 MC:Z:151M AS:i:84 XS:i:37 A00126:224:H7HMWDSX3:3:1114:14986:3239 81 Test_seq 333377 60 124M27S = 333153 -348 CTGCAGTATTCCCTTGTTCTGTCTGACCAACTGCCTCTTGATCTATGTAAAGGTTCCTTATGAGCACAATTAAGTGTTCTGGAATTCCCATTCTTCGCAGTGTTATCCAGAGTTTGTTATGATCGATCCAGGCAGGTAAACCTTCAGGCCA ,:FFFFFF,:FFF:FF:,FFFFF:F,:F:,FFF,:FF,F:,FFFFFF:F,FFF::FFFFFFFFFFFFF:FF,FFFFFFFFFF:F,:FFFFFFF:FFFF:FFFF:F:FFFFFFFFF::FF:F:FFFFFFF,F:FFFF,FFFFFFFFFF:FFF NM:i:1 MD:Z:0A123 MC:Z:151M AS:i:123 XS:i:62 A00126:224:H7HMWDSX3:3:1114:14986:3239 161 Test_seq 333153 32 151M = 333377 348 GTGGTCTTTGATCTTCATTAGTAAGTGCTTCAAGTCCTCTTCACTTTCAGCAAGCAAGGTTGTGTCATCTGCATAACGCAGGTTGTTAAAGAGTCTTCCTCCAAACCTGATGCCCCGTTCTTCTTCATATAGTCCAGCTTCTCGTATTATT F:FF,FFF:FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFF,FFFFFFFFF:FFF:F:FFFFFF:FFFF:FFFFFFF:F:FFFFFFFFFF:FFFFF:F,::::FFFF:FF:F,,F:FF,FFFFFF:,FFFFF:FFFFF,,:FF:FFFF,,F: NM:i:0 MD:Z:151 MC:Z:124M27S AS:i:151 XS:i:136 XA:Z:Test_seq,+315878,151M,3; A00126:224:H7HMWDSX3:3:1114:26268:3333 81 Test_seq 435936 60 151M = 435684 -403 TTATCGCAGTTGTAAATACCTTTTCTCTCTTTTTCAGGACAGACTGGCAAATCTTTGAATGTCTAATGTACACCTATGTATGATAATCTCTTTTCCTCCAGGAAAAAATTTCTGCCTTGAAAAAGCAAGCGAATCATATTGTCCAGCAGGC FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:26268:3333 161 Test_seq 435684 60 151M = 435936 403 CTGAGCAATAATCACCGCCCATGGGGCAGTTCTGCTCTGCCCTATAGGGTTGCTATGAGTCGGAATCAACTCCAGGGCAATAGGGTTTTTTTGGTTTTACCATCACCTTGAATCAGAAACAACTAGACAGCAATAGCAAATAGCTAAAAGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:31 A00126:224:H7HMWDSX3:3:1114:5791:5008 113 Test_seq 70310 6 131S20M = 71230 1052 GAGACGTGGAGCCAACTTGGCCCAATTCAGTCAAGGTCACGTCCAATGATCACGTCACAGACACCAAATCCTCAGCCGCAAAAGCATGGCGAGAGGGCAGCATGCAGGTGGAAGCAGCCCATTCTAGGATCAATCCAGGTTCTGCCCCTCC FFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:20 MC:Z:151M AS:i:20 XS:i:0 SA:Z:Test_seq,71226,+,20S131M,60,0; A00126:224:H7HMWDSX3:3:1114:5791:5008 2145 Test_seq 71226 60 20H131M = 71230 155 GATCCTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFF NM:i:0 MD:Z:131 MC:Z:151M AS:i:131 XS:i:0 SA:Z:Test_seq,70310,-,131S20M,6,0; A00126:224:H7HMWDSX3:3:1114:5791:5008 177 Test_seq 71230 60 151M = 70310 -1052 CTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCTCCCAGCCTCTAGGAGCTTATGAG FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:131S20M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:24153:6778 97 Test_seq 166090 60 151M = 166418 464 GTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCT FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,F:F:FFFFF,FF::FFFFFFFFFFFF:FFFFFFFFFF:FF,: NM:i:1 MD:Z:134G16 MC:Z:136M15S AS:i:146 XS:i:85 A00126:224:H7HMWDSX3:3:1114:24153:6778 145 Test_seq 166418 60 136M15S = 166090 -464 GCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAATCTCTTTGCTTTTAGTCCAGTCCAGTTGGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCGATCCACCGTCAATC FFFFFFF,FFFFFFFFFFFFFFFF:F:FF:FFFFFFFF,FFF:FFFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:39C23A2A20T48 MC:Z:151M AS:i:116 XS:i:40 A00126:224:H7HMWDSX3:3:1114:29423:7827 97 Test_seq 168477 60 151M = 15873 -152586 GCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:18T132 MC:Z:131S20M AS:i:146 XS:i:92 A00126:224:H7HMWDSX3:3:1114:29423:7827 145 Test_seq 15873 6 131S20M = 168477 152586 TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCACCCTTTGAAATCTTCTGTTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:0 MD:Z:20 MC:Z:151M AS:i:20 XS:i:0 SA:Z:Test_seq,168685,-,126M25S,60,2; A00126:224:H7HMWDSX3:3:1114:29423:7827 2193 Test_seq 168685 60 126M25H = 168477 -334 TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:2A28T94 MC:Z:151M AS:i:118 XS:i:58 SA:Z:Test_seq,15873,-,131S20M,6,0; A00126:224:H7HMWDSX3:3:1114:22453:9032 97 Test_seq 414176 60 151M = 414303 278 TGCCTCTTTGTCCACCTAGAAGCTTAGACAAGAAACCTGGAAGTCAGTCTTGATCCTTCTTCGTCCATCACCCCCACATCCAGTCGGTTATCAAAATACTTCTCAATTACTTCAACCTCTTCGCATCTCCACTGACAGCATTCTCCCCTCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF,FF:FFFFF::FF:FF,FFFFFFFFFFF:F:FFFF,FFFFFFFFFFFFFF,,FF,FFFF:FF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:22453:9032 145 Test_seq 414303 60 151M = 414176 -278 TCCACTGACAGCATTCTCCCCTCAATCACCATCGACTCGCATTAAGCCCCATTTAGTTCCATTTCCCTTTCAATCCATCTCTTCACAGCAGCCAGGGTGATTTAAAAAAATATATAAATTACAGCACATCACTCTGGTGTGTGGCCCACTG FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:27959:9392 97 Test_seq 171239 60 151M = 171507 419 GTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGATTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTG F,,FF:FFFFFFF,:FF:FF:FFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFF,F,FFFFFF,FFFFFFFFFFFFF,FFFFFFFFF:FF:FFFFFFFF:FFF,FFFF:FFFFFFFFFFFFF:FFFFF,,:,:FFFFFFFFFF,FFFFFFFF NM:i:1 MD:Z:63G87 MC:Z:151M AS:i:146 XS:i:65 A00126:224:H7HMWDSX3:3:1114:27959:9392 145 Test_seq 171507 60 151M = 171239 -419 TTTTTTCAGCTATGTTGCAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGGTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:17G46C11T74 MC:Z:151M AS:i:136 XS:i:47 A00126:224:H7HMWDSX3:3:1114:13259:9518 81 Test_seq 306104 60 136M15S = 305947 -293 TCATCACAAAGGTCTGCGGTTGGTAAAGAGAGGAACTGGATGAAAGGGTTTTGATGGGTTGTTCAAATCTACCACCAAGGCTGTAAACAACTCCCTGAATGAAAGCATCATACTTACTCTTCTTCTAGCCTGGATTTAAAAAAGGGGGGAA FFF:FFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFF,FFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFF,FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:136 MC:Z:151M AS:i:136 XS:i:0 A00126:224:H7HMWDSX3:3:1114:13259:9518 161 Test_seq 305947 60 151M = 306104 293 GAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATACTAAAGAGGCAAAAGTCATGGAAACCACTGGAGGGCCATTATCTTTCCTCATCTCCCCCATGTTGC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:F:FFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:136M15S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:27199:9643 97 Test_seq 171239 60 151M = 171507 419 GTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGATTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTG F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFF NM:i:1 MD:Z:63G87 MC:Z:151M AS:i:146 XS:i:65 A00126:224:H7HMWDSX3:3:1114:27199:9643 145 Test_seq 171507 60 151M = 171239 -419 TTTTTTCAGCTATGTTGCAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGGTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:17G46C11T74 MC:Z:151M AS:i:136 XS:i:47 A00126:224:H7HMWDSX3:3:1114:26341:10097 97 Test_seq 169294 60 151M = 169620 453 TGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F,FFFFFFFF NM:i:1 MD:Z:15T135 MC:Z:127M24S AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1114:26341:10097 145 Test_seq 169620 60 127M24S = 169294 -453 TTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTAATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATCAATCCTCTTTTCTTACTC FF:FFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F:FFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:4 MD:Z:8T11A21G0G83 MC:Z:151M AS:i:107 XS:i:72 A00126:224:H7HMWDSX3:3:1114:18647:10394 81 Test_seq 171514 60 151M = 171286 -379 AGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGAT FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:69T81 MC:Z:30S121M AS:i:146 XS:i:56 A00126:224:H7HMWDSX3:3:1114:18647:10394 161 Test_seq 171286 60 30S121M = 171514 379 TGTGCAACCATTCTTACCCTCTTTTTCTAAATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF:FFFF,F,FFF NM:i:1 MD:Z:114G6 MC:Z:151M AS:i:116 XS:i:43 A00126:224:H7HMWDSX3:3:1114:8006:11130 97 Test_seq 168738 60 151M = 168961 374 AAAGCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFF:F::FFF,F:FF,F:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FF:FFFFFFF:FFFFFFFF,F,FFFFFFFFFFFFFF, NM:i:1 MD:Z:3C147 MC:Z:151M AS:i:147 XS:i:81 A00126:224:H7HMWDSX3:3:1114:8006:11130 145 Test_seq 168961 60 151M = 168738 -374 TCTATGCTTTGAAATACCTTCAGTAGTAATGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCTGTCTGGGCCAGGGCCTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTAT FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:28G47C12A1T59 MC:Z:151M AS:i:131 XS:i:81 A00126:224:H7HMWDSX3:3:1114:8223:11193 97 Test_seq 168738 60 151M = 168961 374 AAAGCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFF:F:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF NM:i:1 MD:Z:3C147 MC:Z:151M AS:i:147 XS:i:81 A00126:224:H7HMWDSX3:3:1114:8223:11193 145 Test_seq 168961 60 151M = 168738 -374 TCTATGCTTTGAAATACCTTCAGTAGTAATGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCTGTCTGGGCCAGGGCCTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTAT F,FFFFF:FFFFF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:28G47C12A1T59 MC:Z:151M AS:i:131 XS:i:81 A00126:224:H7HMWDSX3:3:1114:21658:14043 97 Test_seq 167949 60 151M = 168149 331 AAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:111C39 MC:Z:10M1D120M21S AS:i:146 XS:i:80 A00126:224:H7HMWDSX3:3:1114:21658:14043 145 Test_seq 168149 7 10M1D120M21S = 167949 -331 TTCTTAAGTATTTATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTGATCTCCTTTTTGATGTCTGG F:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:3C6^T9C101T8 MC:Z:151M AS:i:110 XS:i:105 XA:Z:Test_seq,+212759,21S117M1D13M,5; A00126:224:H7HMWDSX3:3:1114:9028:14058 97 Test_seq 169008 60 146M5S = 169331 474 AAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTAGTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFF,FFFFFFFFF,FFFFFF:FFF,,FFFF,F:F,F,F:F,FFFF,FF:,:,FF,FF:F:FFF:F,FFF,,F,F,F,F,F,:FFF:FFF,FFFFFFF NM:i:2 MD:Z:0G115C29 MC:Z:151M AS:i:140 XS:i:67 A00126:224:H7HMWDSX3:3:1114:9028:14058 145 Test_seq 169331 60 151M = 169008 -474 ATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTC F:FFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:F:FFF NM:i:0 MD:Z:151 MC:Z:146M5S AS:i:151 XS:i:111 A00126:224:H7HMWDSX3:3:1114:20925:15186 97 Test_seq 168989 60 151M = 169319 481 GTGGTGTTAACTCTTCTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,FFFFF,FFFFFFFFFFFFFFFF::::FFFFF,F:FFF,:F:FFFFF,:F,F,FFFF,FFFFFFFFFFFF,F,F,F:F NM:i:4 MD:Z:10G11G38A14A74 MC:Z:151M AS:i:131 XS:i:85 A00126:224:H7HMWDSX3:3:1114:20925:15186 145 Test_seq 169319 60 151M = 168989 -481 TCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATT :FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:148G2 MC:Z:151M AS:i:148 XS:i:112 A00126:224:H7HMWDSX3:3:1114:13684:16986 73 Test_seq 209433 11 53M = 209433 0 TCCCTCCTGACAACATGTCCAAAGTATGTAAGACGCAGCCTCGCCATCCTTGC FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,FF,F,FFF,FFFFFFFFFF NM:i:1 MD:Z:38T14 AS:i:48 XS:i:43 XA:Z:Test_seq,-240774,53M,2;Test_seq,+332659,53M,2; A00126:224:H7HMWDSX3:3:1114:13684:16986 133 Test_seq 209433 0 * = 209433 0 GCAAGGATGGTGAGACCGCATCTTACATACTTTGGTCATGTTGTCAGGAGGGA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF MC:Z:53M AS:i:0 XS:i:0 A00126:224:H7HMWDSX3:3:1114:4372:17425 113 Test_seq 168509 60 151M = 212625 44103 ATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGC FFF:FFFFF:FFFFFFF:FFFF:FFFFFFFFF:FFFFFFFFFF,,FFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFF,FFF::FFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF: NM:i:1 MD:Z:100A50 MC:Z:137M14S AS:i:146 XS:i:83 A00126:224:H7HMWDSX3:3:1114:4372:17425 177 Test_seq 212625 29 137M14S = 168509 -44103 AAAGAAATAAAGGGTATCCGGATTGGCAAGGAGGAAGTAAAGTTATCACTATTTGCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTGATCACATGACAGA FF,FFF:FFFFFF:F:FFFFFF:FFFFF,FFF:FFFFFFFF:FF,FFF:FF:FFF:,FFFF:FFFFF:FFFFFFFFF:FFF::,FFF:F,,FFFFFF,FF,FF:FF::FFFF,,FFFFFFF:FFFFFF:FFFFFFFFFFF:F:,F:F:,FF NM:i:5 MD:Z:14C3T22A5T39T49 MC:Z:151M AS:i:112 XS:i:97 XA:Z:Test_seq,+168277,14S137M,8; A00126:224:H7HMWDSX3:3:1114:6126:18145 81 Test_seq 383367 60 126M25S = 383221 -272 TAAAGTATTATGACATGTGCAAAGAGCTGGAGATGGAAAACCAAAAGGGAAGAACATGCTTGGCATTTCTCCAGCTGAAAGAACTGAAGAAAAAGTTGAAGCTTCGAGTTGCAATAGTGAAGGATTTAAAACATTACCTGCAAAGGTAAAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:126 MC:Z:151M AS:i:126 XS:i:0 A00126:224:H7HMWDSX3:3:1114:6126:18145 161 Test_seq 383221 60 151M = 383367 272 ACAGGAAAGAATGAAAAGACCAAGATGGATGTCAGAGGAGACTCTGAAACTTGCTCTCTAATGTTGAGCCGCTAAAGCAAAAGGAAGAATTGATGAAGTAAAGGAACTGAACAGAAGATTTCAAAAGGCAGCTCGAGAAGACAAAGTAAAG FF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:126M25S AS:i:151 XS:i:79 A00126:224:H7HMWDSX3:3:1114:31195:18411 97 Test_seq 168238 17 22S129M = 168451 364 CTAGCAGTGGTGTCCCTTGATCAATCCAAGTGATTTTTGTATGGTTATTTTATAACCTGAGACTCTTCCAGACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCGTGTATACGATCATGTCATCTGCAAATAGT FFFFFFFFFFFFFFFF,,FFFFFFFFFFFFFFFF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:21T11T14A50A8G18A1 MC:Z:151M AS:i:102 XS:i:93 XA:Z:Test_seq,-212672,129M22S,8; A00126:224:H7HMWDSX3:3:1114:31195:18411 145 Test_seq 168451 60 151M = 168238 -364 ATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA :FFFFFFFF,FFFFFFFFFFFFF,FFFFFFFF,FFFFFFFF,FFFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FF:FF:FFFFFFFFFFFFFFFFF,FFFFF NM:i:0 MD:Z:151 MC:Z:22S129M AS:i:151 XS:i:87 A00126:224:H7HMWDSX3:3:1114:30834:18724 81 Test_seq 169458 60 151M = 169313 -296 GCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGTAGGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTACATTTATCAATATAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGA FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:6 MD:Z:46C0A13C0C28G8G50 MC:Z:151M AS:i:121 XS:i:71 A00126:224:H7HMWDSX3:3:1114:30834:18724 161 Test_seq 169313 60 151M = 169458 296 CTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACAAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGA F:FFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F,FFFFFFFF NM:i:1 MD:Z:53C97 MC:Z:151M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1114:23339:18771 81 Test_seq 167439 60 133M18S = 167331 -241 ATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATTTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCAAGTAAAATGAAA FFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:72T23C36 MC:Z:151M AS:i:123 XS:i:85 A00126:224:H7HMWDSX3:3:1114:23339:18771 161 Test_seq 167331 23 151M = 167439 241 TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFFFF:F,FFFFFFFFF:FFFFFFFFFFF:FFFFF::F,F,FFFFF,:F:FF NM:i:1 MD:Z:37A113 MC:Z:133M18S AS:i:146 XS:i:135 XA:Z:Test_seq,-213553,151M,4; A00126:224:H7HMWDSX3:3:1114:22806:19194 81 Test_seq 166056 60 151M = 165892 -315 GTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCAGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGG FFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFF:F,FFF:FFFFFFFFFFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF NM:i:1 MD:Z:78G72 MC:Z:10M2D141M AS:i:146 XS:i:76 A00126:224:H7HMWDSX3:3:1114:22806:19194 161 Test_seq 165892 60 10M2D141M = 166056 315 GCATCTCTCTCCCCTCCACTGACCAGTCCTCTCCATGCCTGATGATTTGTCTTCGGGGATGGTTCCTGTCTTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTAGGATTCCTCTAGTCGCAGTCATACCATTATGTATGGTCTTTTCAT FFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFF:FFFFFFFF:FFFFFF:FFFFFFFFFFFFF:FFFF,FFFFFF:FFFF,:FFFFFFFFFFFFF,,FFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFF,FF:FFFFFFFFF:FFF, NM:i:15 MD:Z:10^CC4C2G12T1T4T7G24C34G14A2T5T5G12T2 MC:Z:151M AS:i:80 XS:i:0 A00126:224:H7HMWDSX3:3:1114:24243:19304 97 Test_seq 73194 60 32M119S = 92225 19182 AAAGGAAGAGCATTCATTCAGAGGACATTTAAGTCACCCTTAACGTCCATAATTCTACCAACTGCCTCTTCGAGGCAGTCTAGGCTTTGACTATCGAGTGCATCGAACCCCTCCAGCCTCTACCCATTATCCAATTCCAAAACCACTTCCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF NM:i:0 MD:Z:32 MC:Z:151M AS:i:32 XS:i:0 SA:Z:Test_seq,92047,+,31S120M,60,0; A00126:224:H7HMWDSX3:3:1114:24243:19304 2145 Test_seq 92047 60 31H120M = 92225 329 AGTCACCCTTAACGTCCATAATTCTACCAACTGCCTCTTCGAGGCAGTCTAGGCTTTGACTATCGAGTGCATCGAACCCCTCCAGCCTCTACCCATTATCCAATTCCAAAACCACTTCCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF NM:i:0 MD:Z:120 MC:Z:151M AS:i:120 XS:i:0 SA:Z:Test_seq,73194,+,32M119S,60,0; A00126:224:H7HMWDSX3:3:1114:24243:19304 145 Test_seq 92225 60 151M = 73194 -19182 CCACCTAGTGCTGCCATAACAAAAGATACCACAAGGGTGTAGCTTTAAAGAACAGAAATTGGTTTTCTCACAGGCCAGGCCAAGCCCGTTGCCATCAAGCTGATGCCCACACGTGGTGACCCTGCGTGTGTCGGAGGAGAACTGTGCCCCC FFFFFFFFFFF:FFFF,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:32M119S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:19633:20400 81 Test_seq 168016 56 151M = 167895 -272 ATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTTTAGTTTTCTTTGTATAGGTCTTTTACATCCTTGGTAAGATTTATTCCTAAGTATTTTATCT ::FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:44C44A13G12C5C28 MC:Z:151M AS:i:126 XS:i:96 A00126:224:H7HMWDSX3:3:1114:19633:20400 161 Test_seq 167895 60 151M = 168016 272 CCGGGGCTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFF NM:i:1 MD:Z:6G144 MC:Z:151M AS:i:146 XS:i:67 A00126:224:H7HMWDSX3:3:1114:26802:21355 81 Test_seq 170423 60 151M = 170234 -340 GCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGACAATTTCATTTTTCCACATACTTTTTTGTGCTGAGGCGTTTTTCTTAGTAAATTGTGAGATCCTCATTTTCATAGTGTTTGACTTT FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:64G1C0T32A14G23A5A5 MC:Z:151M AS:i:116 XS:i:48 A00126:224:H7HMWDSX3:3:1114:26802:21355 161 Test_seq 170234 60 151M = 170423 340 TTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFF:FFFFFF:FFF:F:FFFFFFFF NM:i:1 MD:Z:28A122 MC:Z:151M AS:i:146 XS:i:78 A00126:224:H7HMWDSX3:3:1114:9480:22169 81 Test_seq 212860 60 16M1I9M3I122M = 212718 -289 TAGCCCCCAAGAAGATAAAAATACTTAGGAGGAATAAATCTTACCAAAGACATAAAAGACCTATACGAAGAAAACTACAATGTACTACTGCAAGAAACTAAAAAGGACCTACTTAAGTGGAAAAACATACCTTGCTCATGGATAGGAAGAC :FFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFF:FFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF NM:i:10 MD:Z:46T0G14A7T5A8A61 MC:Z:151M AS:i:101 XS:i:44 A00126:224:H7HMWDSX3:3:1114:9480:22169 161 Test_seq 212718 52 151M = 212860 289 TCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:119A31 MC:Z:16M1I9M3I122M AS:i:146 XS:i:121 XA:Z:Test_seq,-168170,151M,6; A00126:224:H7HMWDSX3:3:1114:8594:22639 81 Test_seq 214119 11 50M = 214119 -50 CAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCT :,FFFFF,FFFF,F,:FF,F,,FF:FF:FFFF:,FF,FFF:F:F:FFF,F NM:i:1 MD:Z:0T49 MC:Z:50M AS:i:49 XS:i:45 XA:Z:Test_seq,+166866,50M,1; A00126:224:H7HMWDSX3:3:1114:8594:22639 161 Test_seq 214119 24 50M = 214119 50 CAAGAGCCTTCATACAAACAGATATATGCACACCCATGTTTATGGCAGCT FFF::FFFFFFFFFF:FFFFFFFFFFF:FFFFFF:FF:FFFFF:FFF::F NM:i:3 MD:Z:0T11C30T6 MC:Z:50M AS:i:39 XS:i:31 A00126:224:H7HMWDSX3:3:1114:9335:22952 81 Test_seq 171539 60 127M24S = 171244 -422 CCCTCCGGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGGCTTTCTATTGCGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTACTCAGGCTAAGATTTTTGGTATT FFF:FFF,FFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:0T5A37T5A17T58 MC:Z:151M AS:i:106 XS:i:47 A00126:224:H7HMWDSX3:3:1114:9335:22952 161 Test_seq 171244 60 151M = 171539 422 GGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFF,FFFFFFFFF:F NM:i:0 MD:Z:151 MC:Z:127M24S AS:i:151 XS:i:66 A00126:224:H7HMWDSX3:3:1114:30264:23156 97 Test_seq 166091 60 151M = 166413 473 TTGTCTGTTGTGTTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCAAGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FF,:FFF:F:,FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFF NM:i:3 MD:Z:12C80T39G17 MC:Z:151M AS:i:136 XS:i:75 A00126:224:H7HMWDSX3:3:1114:30264:23156 145 Test_seq 166413 60 151M = 166091 -473 CCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCAATAAAAAAC FFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:30 A00126:224:H7HMWDSX3:3:1114:26386:26052 97 Test_seq 336841 60 151M = 337069 379 GTTACACTCTGTAACACATGGGGTTGCCATGAGTCAGAATCTACTCAATGGCAATGGGTTTTTTGTTTTTGGTTTACCTCTTAGGGTTGTTATCAGGATTAAACAAACTAATACTATATTTGGAAGAGTACCTGGTGTGTATAATAACTGT FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:26386:26052 145 Test_seq 337069 60 151M = 336841 -379 TTGTTAGTATGAATGTATGCACCTTAGACCCTCAAAAGAGAGAACGACTGTCTCATAGATGTCTACTTAGTCAAGTGTATTGCTAGTGTTGTCCTGGGCGATGTTTGAAACCATAGTGCTTCAGGTCGAGGCTGTTAATTCATTCTTGATC FFFFF,:FFF:FFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:6424:26209 97 Test_seq 166060 60 151M = 166386 477 GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,F:FFFF,FFFFFFFFF:F NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:57 A00126:224:H7HMWDSX3:3:1114:6424:26209 145 Test_seq 166386 60 151M = 166060 -477 CCTCAAACCAAGGTCCCCGGACCTCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCCTTTAATCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTC FFFFFFFFF:F:,FFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:10T1T5A4C56T4G33T31 MC:Z:151M AS:i:116 XS:i:32 A00126:224:H7HMWDSX3:3:1114:14534:27164 81 Test_seq 153478 12 54M = 153478 -54 TCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGAAATGATT FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:2 MD:Z:38G8T6 MC:Z:54M AS:i:44 XS:i:39 XA:Z:Test_seq,-51125,54M,3; A00126:224:H7HMWDSX3:3:1114:14534:27164 161 Test_seq 153478 13 54M = 153478 54 TCTAGGTTTTCAAATTTGGTAGAGTACAATTTTTCATAGTAATCTGATATGATT FFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:18T35 MC:Z:54M AS:i:49 XS:i:44 XA:Z:Test_seq,+51125,54M,2; A00126:224:H7HMWDSX3:3:1114:23936:27602 81 Test_seq 169843 60 34M1D35M1D82M = 169575 -421 TGGTCTATTCTAGAGAATGTTCCATGTGCACTAGAAAAAAAGTATATTTTGCAGCAGTTGGGTGGAGAGTCTGTATAAGTCAATGAGGTCAAGTTGGTTGATTGTTGTAAGTAGGTCTTCCGTGTCTCTATTGAGCTTCTTACTGGATGTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFF NM:i:5 MD:Z:23C10^A35^T12T47G21 MC:Z:151M AS:i:122 XS:i:47 A00126:224:H7HMWDSX3:3:1114:23936:27602 161 Test_seq 169575 60 151M = 169843 421 GAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTTGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFF: NM:i:1 MD:Z:88G62 MC:Z:34M1D35M1D82M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1114:5864:28119 97 Test_seq 167568 31 151M = 167902 462 GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAAAATCAGC FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFF::F:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:: NM:i:1 MD:Z:144T6 MC:Z:128M23S AS:i:146 XS:i:131 XA:Z:Test_seq,-213320,140M2I9M,4; A00126:224:H7HMWDSX3:3:1114:5864:28119 145 Test_seq 167902 60 128M23S = 167568 -462 TTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTGATCATTCAAAAGTGGTTGCAGC FFFFFFFF::FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:128 MC:Z:151M AS:i:128 XS:i:0 A00126:224:H7HMWDSX3:3:1114:28212:28808 97 Test_seq 168997 60 151M = 169318 472 AACTCTTCTCTGAAAATTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:F:FFFF:FFFFF::FFFFFFF:FFF,FFFFFF,FFFF:FFFFFFFFF:FFFFF,FF::FFF:FFFF:FFFF::FF:FFFFFFFFF::FFFF:FFFFFFFF:FF,,FF:FFF: NM:i:6 MD:Z:2G11G0G28T23A57A24 MC:Z:151M AS:i:123 XS:i:65 A00126:224:H7HMWDSX3:3:1114:28212:28808 145 Test_seq 169318 60 151M = 168997 -472 GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT FFFFFFFFFFFF,FF:FFFFFFFF:FFFFFF:FFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:149G1 MC:Z:151M AS:i:149 XS:i:112 A00126:224:H7HMWDSX3:3:1114:10176:29168 81 Test_seq 161204 60 151M = 161012 -343 ACATACAGCTAACTTACCACACACCAGAGAGGCCCGTGGGCCACCTCAGGGGCTGGAACCCCCTGCAGTGACACACGGCATCTTAAATGCATGTGCGTGTTTTCTGTAAAGGATCCACAGCATTCGTCTGATTCTCAAAGGAGCCTGTGAT :,FF::F:FFFFF:FFFF:FFF:FFFFFFFFFFFFFFF:,FF::FF:FFFFFF:,FFFFF:FFFFF,F:FFFFFFFFF:FFFFFFFFF:FF::F:FFFFFFFFFFFFFF:FFFFFFFF,,:FFF,FFFFFFFFFFFFFFF::FFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:10176:29168 161 Test_seq 161012 60 151M = 161204 343 TTGGGAGCCCCTCTGTTCCCTGTCTTACTGAGTCCCTAGGTTCTATACCCAACCCCCTGTTACCTCCTTGATAGTATAGCCTATCTCTCTTTTACTTGCTTCTCCTCTAACCACACCAGTCTCTTTGCTGTTCCTCAACAGGTGCCCTCCG FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FF:FFFFFF,F::FFFFFFFFFF:FF:FFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:51 A00126:224:H7HMWDSX3:3:1114:28266:30436 97 Test_seq 168757 60 151M = 168917 311 GGTATAAATCCCACTTGATCGGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTACCTGGTTTTG FF:FFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFF,FFF,:FFFFFFFFFF,FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFF NM:i:1 MD:Z:20A130 MC:Z:151M AS:i:146 XS:i:82 A00126:224:H7HMWDSX3:3:1114:28266:30436 145 Test_seq 168917 60 151M = 168757 -311 AGATGGTAGCTTCATAGAAGGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTAAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAG FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:7G11T22G48G29G29 MC:Z:151M AS:i:126 XS:i:67 A00126:224:H7HMWDSX3:3:1114:7075:30906 81 Test_seq 168748 60 151M = 168547 -352 TGCATACCTGGTACAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAATGTCTTTAC FFFFFFFFFFFF:FFFFFF::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF NM:i:1 MD:Z:13T137 MC:Z:151M AS:i:146 XS:i:81 A00126:224:H7HMWDSX3:3:1114:7075:30906 161 Test_seq 168547 60 151M = 168748 352 GTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTGTCTTTTGTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFF NM:i:1 MD:Z:140A10 MC:Z:151M AS:i:146 XS:i:86 A00126:224:H7HMWDSX3:3:1114:21838:31955 97 Test_seq 165871 60 31M3D105M15S = 166132 412 TAATTAGCTCACTCTTCATCAGCATCTCTCTCCCCCCACTGACCAGTCCTTTTCATGCCTGATGATTTGTCTTCGGGGATGGTTCCTGTCCTGTGCCATCAGAAGTTCTGGGGAGCATTGTCTCTGGGATTCCTCTGGTCGCAGTCATACC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:7 MD:Z:5G25^CCC6G19T7G70 MC:Z:151M AS:i:107 XS:i:0 A00126:224:H7HMWDSX3:3:1114:21838:31955 145 Test_seq 166132 60 151M = 165871 -412 CCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTT FFFFFFFFFF,F:FF:FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:92G58 MC:Z:31M3D105M15S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1114:27760:32002 81 Test_seq 197256 60 148M3S = 196937 -467 CTCAACTTCCAGATGGTAAGAAATTTTGTGAGAGTGATGAAATTAAGATACTTGATGCGTCACAGGCCAGGGCAGCAGCACTGCTCAATTCTAGGGGGAATTGCTTCCATGACAGTGTATTGAACCTGTGCTCCAAGGGGTGCCTTTATAG FFFFFFFFFFFFFFF:F:FFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFF:FFFFF:FFFFFFFFFFFFF::FFFFFFFF NM:i:0 MD:Z:148 MC:Z:151M AS:i:148 XS:i:0 A00126:224:H7HMWDSX3:3:1114:27760:32002 161 Test_seq 196937 60 151M = 197256 467 CACCATTAACTCCTGAAAGCACTCACACACATCCTACCCAACACAACTAAAATAATTCCACCATCTGAGACTCTTATGGAAGAAATGTTACCAAATACAAAGTGCAAGGTAAAATAGCATGGTCTTCTGGACATTTTTCCCTCCCTTGTGC FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFFFFF:FFFFFFFFFFF,FFFFFFF:,FFFFFFFFF,FF NM:i:0 MD:Z:151 MC:Z:148M3S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:28763:32550 97 Test_seq 169079 46 151M = 169314 386 CGGTTTCAATCTCTTTTTTTGTTCTGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCTAGGAATTCATCCATTTCTTCTAGGTTTGCAAATTTGTTAGAGTACAATTTTTCATAAGAATCTGAAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFF NM:i:10 MD:Z:1C21A58G1A25T11C11A1G2G0T10 MC:Z:151M AS:i:107 XS:i:80 A00126:224:H7HMWDSX3:3:1114:28763:32550 145 Test_seq 169314 60 151M = 169079 -386 TTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGAT FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF: NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:112 A00126:224:H7HMWDSX3:3:1114:30436:32628 97 Test_seq 167332 9 109M = 400753 233528 GAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:36A72 MC:Z:107M2S AS:i:104 XS:i:99 XA:Z:Test_seq,-213594,109M,2;Test_seq,+400753,107M2S,3; A00126:224:H7HMWDSX3:3:1114:30436:32628 145 Test_seq 400753 3 107M2S = 167332 -233528 GAGATGTCATAGCTGCAAATTTTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGAGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTAT FFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:15A2G10G28T48 MC:Z:109M AS:i:87 XS:i:84 XA:Z:Test_seq,+213594,109M,5;Test_seq,-167332,109M,6; A00126:224:H7HMWDSX3:3:1114:28393:32878 113 Test_seq 212712 11 53M = 168274 -44439 AAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTC FFF,FFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFF::FFFFFFFFFFFFFFF NM:i:1 MD:Z:0T52 MC:Z:53M AS:i:52 XS:i:48 XA:Z:Test_seq,+168274,53M,1; A00126:224:H7HMWDSX3:3:1114:28393:32878 177 Test_seq 168274 13 53M = 212712 44439 GAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTTCTT FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:49C3 MC:Z:53M AS:i:49 XS:i:44 XA:Z:Test_seq,+212716,4S49M,1; A00126:224:H7HMWDSX3:3:1114:16586:33223 81 Test_seq 167154 44 151M = 166961 -344 CATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF NM:i:1 MD:Z:89T61 MC:Z:151M AS:i:146 XS:i:125 XA:Z:Test_seq,+213730,151M,6; A00126:224:H7HMWDSX3:3:1114:16586:33223 161 Test_seq 166961 6 151M = 167154 344 GTAGTTCTATTTCTAACTGTTTAAGATAATGCCAGATAGATTTCCAAAGTGGTTATACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCTGCAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:F:FFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:7 MD:Z:29C7G16G12A27G6C0A47 MC:Z:151M AS:i:116 XS:i:111 XA:Z:Test_seq,-213923,151M,8; A00126:224:H7HMWDSX3:3:1114:18105:33536 97 Test_seq 167102 44 26S125M = 167412 461 TTAATGTTTCTGTGGCTGAAGTTGAGTTAATGCCAGTCTAGTTGGTGTGAGATGGAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCCTTAGTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF NM:i:2 MD:Z:42C75T6 MC:Z:151M AS:i:115 XS:i:95 XA:Z:Test_seq,-213808,125M26S,6; A00126:224:H7HMWDSX3:3:1114:18105:33536 145 Test_seq 167412 60 151M = 167102 -461 GTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:99T51 MC:Z:26S125M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1114:13801:36354 81 Test_seq 191666 60 151M = 191422 -395 CTTAAAACTAGCCCAAGCCCTCAGCAAACCACAGAAACGTAAACTACCACAGAGGGGATAATCTGGCTGGAGCAGGGTGGAGCGGGTGACCTAACCCGTTTCTCCACCGTGCACCACTGCGAGGGCTCCGGTTCTGCATGCCTCTTGCTTG FFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1114:13801:36354 161 Test_seq 191422 60 151M = 191666 395 TCTCTTGTGGCTGTGTAGCAAGACTGTTAAAGTGCTCAGTGCCTGACAGAAATTTAAACTCCCAATGAGAGATTTTGCTGCAATGGAGTAGAAAGTTGAAATTTAAGAAAAGAAGACTAAGCTGCCTCTCAGAGAATAGCTCCAGAAGGCT FFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1115:10854:1626 97 Test_seq 168347 60 29S122M = 168547 351 AAGAAGGGCTACATGTATTTACAGATCGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTGCCAATCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGT FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:2 MD:Z:18A28T74 MC:Z:151M AS:i:112 XS:i:66 A00126:224:H7HMWDSX3:3:1115:10854:1626 145 Test_seq 168547 60 151M = 168347 -351 GTTGGCTTTGCATAGGTGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTT FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF NM:i:1 MD:Z:15A135 MC:Z:29S122M AS:i:146 XS:i:76 A00126:224:H7HMWDSX3:3:1115:12454:2425 97 Test_seq 169083 60 151M = 169317 385 TTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCGGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCCAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTGGAGTACAATTTTTCATAATAATCTGAAATGATT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFF NM:i:8 MD:Z:45T32G1A36A0C11A1G2G15 MC:Z:151M AS:i:111 XS:i:76 A00126:224:H7HMWDSX3:3:1115:12454:2425 145 Test_seq 169317 60 151M = 169083 -385 AGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGA FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:150G0 MC:Z:151M AS:i:150 XS:i:112 A00126:224:H7HMWDSX3:3:1115:19262:2409 81 Test_seq 167145 42 151M = 166958 -338 TTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGAC FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:F:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:98T52 MC:Z:151M AS:i:146 XS:i:126 XA:Z:Test_seq,+213739,151M,5; A00126:224:H7HMWDSX3:3:1115:19262:2409 161 Test_seq 166958 7 151M = 167145 338 ATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATAGATTTCCAAAGTGGTTGTACCATTTTACATTCCCACCAGCAGTGTATGAGAGTTCCAATCTCTCCGCAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGC FF:FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,:FFFF:,:FFF::FF,FFFFFF:FFFF:F NM:i:4 MD:Z:40G29A27G7A44 MC:Z:151M AS:i:131 XS:i:126 XA:Z:Test_seq,-213926,151M,5; A00126:224:H7HMWDSX3:3:1115:21224:2581 97 Test_seq 169351 60 151M = 169584 384 ATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTT FFFFFFFFFF:FFFFFFFF,:,FFF::FFFFF:F:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFF:FF,FFFFF:F:,F:FFFFFFFFFFFFFFFF,FF,FF:,F,FFFFFFFF::FFF:FFFFFFFFFFFF,F,FFF NM:i:1 MD:Z:116G34 MC:Z:151M AS:i:146 XS:i:101 A00126:224:H7HMWDSX3:3:1115:21224:2581 145 Test_seq 169584 60 151M = 169351 -384 TTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF NM:i:4 MD:Z:44T11A22G70A0 MC:Z:151M AS:i:135 XS:i:22 A00126:224:H7HMWDSX3:3:1115:19804:3380 81 Test_seq 169318 60 151M = 169004 -465 GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT :FFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:149G1 MC:Z:151M AS:i:149 XS:i:112 A00126:224:H7HMWDSX3:3:1115:19804:3380 161 Test_seq 169004 60 151M = 169318 465 CTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTACTTAGTTGTTCTACTTCTGAATGTGTTAGGTTAGGTAGGTAGTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFF:FFFFFF:F:FFFFF:F,FFFFFF:,FF,FFF NM:i:5 MD:Z:7G38A14A45T28T14 MC:Z:151M AS:i:126 XS:i:78 A00126:224:H7HMWDSX3:3:1115:20320:5431 81 Test_seq 169318 60 151M = 169004 -465 GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:149G1 MC:Z:151M AS:i:149 XS:i:112 A00126:224:H7HMWDSX3:3:1115:20320:5431 161 Test_seq 169004 60 151M = 169318 465 CTCTGAAAGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGGCTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTACTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTG FFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,F:FFFFFFFFFFFFF,F:FFFFF:FFF:FFFFFFFFFFFFFF,:F:FFF::FF:FF,FFFF:FFFFF NM:i:4 MD:Z:7G38A14A45T43 MC:Z:151M AS:i:131 XS:i:83 A00126:224:H7HMWDSX3:3:1115:8187:6903 81 Test_seq 290434 60 32M1I118M = 290254 -330 GAGCAGGGGCACGTTTGCTCAAGTATAAGGGTGGTAGTGTAGTTTACCATCACCAAGAATTATTAGGATATTAACTCACTTTAGACTTAAGATAGCAGTATGTCACTTAAAGAAATTCCAGACCTAGGCAAGATTTTTGCCTTCAGACCTC FFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:150 MC:Z:151M AS:i:143 XS:i:0 A00126:224:H7HMWDSX3:3:1115:8187:6903 161 Test_seq 290254 60 151M = 290434 330 ATGTGAAGAATGTAACCAATGGCATTGATCTGTATGTGTAGAAATTGTTGAATGGGTGTATGTTATGTATATTTTCAACCAAAATTAAAAAAAAAAAAAAAGAGAAAGAAGAGGATGGGACCAGAAGGGCAAGGCCACAGGGGCATGACCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF,FF:FFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:32M1I118M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1115:12274:7623 97 Test_seq 213781 60 151M = 214075 445 GTGGGCAAAGGATATGAACACGCTCTTCACTAAAGAAGATAGTCAGGCGGCTAACAGATACATGAGAAAATGCTCTCGATTATTAGCCATTAGAGAAATGCAAATTAAAACTACGATGAGATTCCATCTCACTCCAACAAGGCTGGCATTA F:F::F:F,:FFF:,:F:FFF,::FFFFF:FFF::FFFFFFFFFF:F,FFFFFFFFFF:FFFF:FFFFFFF,FFFFFFFFFFFFFF,FFFFF,FFF,:FFF,FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFF,:FFFFFFF: NM:i:5 MD:Z:23A17T6A31C29G40 MC:Z:151M AS:i:126 XS:i:91 A00126:224:H7HMWDSX3:3:1115:12274:7623 145 Test_seq 214075 60 151M = 213781 -445 ATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCATCAAAGGATGAATGG FFF,FFF:FFFFFFF,F:FFFFF::,FFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFF:FF,FF::FFFFF::,FFF:FFFF:FF,F:FFF:FFFFFFF:F,FFFFFFF,F::FFF,FFFFFFFFFFFF:FFF:FFFFFF:F:FFF NM:i:1 MD:Z:140T10 MC:Z:151M AS:i:146 XS:i:103 A00126:224:H7HMWDSX3:3:1115:13892:9987 81 Test_seq 169617 60 130M21S = 169352 -395 ATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTCTTCTGTTATTGATCGATCAGAAAGGTGCC FFFFFFF,FFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:11T11A22G70A3T8 MC:Z:151M AS:i:105 XS:i:0 A00126:224:H7HMWDSX3:3:1115:13892:9987 161 Test_seq 169352 60 151M = 169617 395 TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFF:FFFFF,FFF:FFFFFFFFFFFFFFF:FFFF:FF,FFF:,FFF:F:FFFFFF:FFFFFFFF,,FFFFFFFFF:F:F:,FF:FFFFFFFFF,F,FF, NM:i:1 MD:Z:115G35 MC:Z:130M21S AS:i:146 XS:i:101 A00126:224:H7HMWDSX3:3:1115:16803:13808 81 Test_seq 169319 60 151M = 168997 -473 TCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATT FFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF NM:i:1 MD:Z:148G2 MC:Z:151M AS:i:148 XS:i:112 A00126:224:H7HMWDSX3:3:1115:16803:13808 161 Test_seq 168997 60 151M = 169319 473 AACTCTTCTCTGAAAATTTGGTAGAACTCTGCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFF::F:FFFF:F:FF,FFF,F,:FFF:FFFFFFFFFFFFFF:FF::FFFFFF::::FFFF,,FFFFFF,FFFFFFFFFFFFFF,F:FFFFF,,:FF:F, NM:i:6 MD:Z:2G11G0G28T23A57A24 MC:Z:151M AS:i:123 XS:i:65 A00126:224:H7HMWDSX3:3:1115:4915:16673 97 Test_seq 151752 0 55M = 400946 249249 TTATGCCATGTGTTAGGGCTCCTAGCATTGTCCCTATTTTTTCTTCCATGATCTA FFFFF:F,:F,:FFF:FFFFFFFF,FFFFF,,:F,FFFF,FFF:F,FFFF,,F:F NM:i:4 MD:Z:11A11C0A29T0 MC:Z:55M AS:i:39 XS:i:39 XA:Z:Test_seq,-213497,55M,4;Test_seq,+49445,53M2S,3;Test_seq,+19104,36M1D19M,4;Test_seq,-41170,55M,5;Test_seq,+400946,55M,5; A00126:224:H7HMWDSX3:3:1115:4915:16673 145 Test_seq 400946 60 55M = 151752 -249249 TTATGCCTTGTATTAGGGCTCCTAACGTTGTCCCTATTTTTTCTTCCATGATGTT FFFF::FFFFF:FFF,FF:FFFF,,:FF::F:F:F:FFFF:FFFF:FFFFFF,FF NM:i:1 MD:Z:52C2 MC:Z:55M AS:i:52 XS:i:0 A00126:224:H7HMWDSX3:3:1115:12599:18677 97 Test_seq 219279 60 151M = 219429 301 GAAATTCAGGAAGCAGAGCTTCCTCAAGTCTTAAAGGGTAGGCTCACAAACTCTGGAGTCTCAAAGAGCGGAACCATAGCCTGGAGCCTCAAAGAGTGGAACCACAGCCTCAAAGGGTGAGACCACAGCGTCCTAGGTATCAAAGAGTCAG FFFF::FFFFFFFFF:F:FF:FFFF:FFFFFFFFFFFF:F:,FFFFFFFFF::FFFFF:FFF:F,F:FF::FFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF,,FFFFFFFF,::FFF::,FFFFFFFFF,FF,FF: NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1115:12599:18677 145 Test_seq 219429 60 151M = 219279 -301 GATCTTCAGTAAATCTGTCCTGTTGTGTGGGGCTGCCTTTCACGTGTGCTGGGGAAATGGCCCCAGATCCACTGTCCAAAGCCGTGGGGGTGGGACTGCCATGGCCAAGGGACAGAAGGACAGAGACTGCCGGAGCAGAGGGGGTGGGATC :FFF,FFF,,F,,FFF,,:,FF,,,:FFFFFF,FFFFF:FF,F,,FF,:,FF:,,FF:F,,F,F,:F:FF:F,F:FFFF:F:FF,FFFF:FFF,FFFFF,FFFFFF,,F:FF:F,F,FFFF,:F,FFF::,F:FFFF,,:F,FF,:FFFFF NM:i:5 MD:Z:8C13G0A20A39A66 MC:Z:151M AS:i:126 XS:i:0 A00126:224:H7HMWDSX3:3:1115:4652:19038 65 Test_seq 261704 26 60M = 214124 -47581 TGCTCTTGTAAACAGAGCTGCAATAAACATGGGCGTGCATGTATCTGTTCGTGTAAAGGC FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFF NM:i:3 MD:Z:4A21G6T26 MC:Z:60M AS:i:45 XS:i:35 A00126:224:H7HMWDSX3:3:1115:4652:19038 129 Test_seq 214124 32 60M = 261704 47581 GCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:60 MC:Z:60M AS:i:60 XS:i:50 XA:Z:Test_seq,-166851,60M,2; A00126:224:H7HMWDSX3:3:1115:14696:21057 81 Test_seq 169097 60 137M14S = 168933 -301 TTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTCGTTTAGGTAGGTAGTATTTTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACATAGTAATCTGAAATGATTTAAGCAGATGTCTT :FFFF,FFFFFF,FFFFFFFFFFFFF::,FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFF:FF:FFFFFF:FFFFFFF,FFF:F NM:i:6 MD:Z:41A15G6G7C31C13G18 MC:Z:151M AS:i:107 XS:i:71 A00126:224:H7HMWDSX3:3:1115:14696:21057 161 Test_seq 168933 60 151M = 169097 301 GAATGAGTTGGGTAGTATTCCGTCTTTTTCTATGCTTTGAAATACCTTCAGTAGTAGTGGTGTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTT FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:FFF:FF:FFFFFFFFFFF:F,FFFF:FFFFFFFFFFFF,:::FFFFFFFF,FFF:FFFFFFFF:FFFF NM:i:1 MD:Z:26G124 MC:Z:137M14S AS:i:146 XS:i:75 A00126:224:H7HMWDSX3:3:1115:17580:21605 81 Test_seq 168019 60 138M13S = 167749 -408 TTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGCAGAGTTTTATAGTTTTCTTTGTATAGGTCTTTTACATTCTTGGTAAGATTTATTCCTAAGAGCATTGGCATTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF,FFFF,FFFFF,FFFF::FFFFFFFFFFFFF:FFFFF,FFFFFFFFF:FFF:FFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFF::FFFFFFFFFFFF NM:i:5 MD:Z:41C58G12C1C3C18 MC:Z:151M AS:i:113 XS:i:78 A00126:224:H7HMWDSX3:3:1115:17580:21605 161 Test_seq 167749 1 151M = 168019 408 TTCTCAATTCTGTTCCATTGGTCTATGTGCCTGTTGTTGTACCAGTACCAGGCTGTTTTGACTACTGTAGCTGTATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGG :FFFFF,,:FFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFF:FFFFFFF:FFFFFFFFFF,FFFFFFFFFFFFFFFF,FF:F:,FFF,FFF:FFFF:FFFF:FFFFF:FF:F:F NM:i:1 MD:Z:69C81 MC:Z:138M13S AS:i:146 XS:i:144 XA:Z:Test_seq,-213139,151M,2; A00126:224:H7HMWDSX3:3:1115:28266:23422 81 Test_seq 167814 60 136M15S = 167524 -426 TGTAGCTATATAATAGGTTCTGAAATCAGGTAGGGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGTTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTACTCATAACCATTCAT FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:4C2G25A37C64 MC:Z:151M AS:i:118 XS:i:81 A00126:224:H7HMWDSX3:3:1115:28266:23422 161 Test_seq 167524 52 151M = 167814 426 TCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTAT FFFFFFFFFFFFFFFFFFF::FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFF,F NM:i:1 MD:Z:120T30 MC:Z:136M15S AS:i:146 XS:i:121 XA:Z:Test_seq,-213364,96M2I23M2I28M,6; A00126:224:H7HMWDSX3:3:1115:28583:23719 81 Test_seq 166429 60 125M26S = 166075 -479 CTTTGAAGCTTTCATTTTATCCCGGAAACCTTTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGACTGATCGATCAGGGGAGAGCTAATACACTTAT FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:31C44T42T5 MC:Z:151M AS:i:110 XS:i:40 A00126:224:H7HMWDSX3:3:1115:28583:23719 161 Test_seq 166075 60 151M = 166429 479 CCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGAC FFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF:F:FF:FFFFFFFF NM:i:1 MD:Z:149G1 MC:Z:125M26S AS:i:149 XS:i:46 A00126:224:H7HMWDSX3:3:1115:22878:24486 81 Test_seq 167514 49 151M = 167180 -485 CCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGGTATGCCGGCACCATTTGTTA FFFFFF:FFFFFFFFF:FFFFFFF,FFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:0T129T6A13 MC:Z:151M AS:i:140 XS:i:116 XA:Z:Test_seq,+213374,86M2I23M2I38M,7; A00126:224:H7HMWDSX3:3:1115:22878:24486 161 Test_seq 167180 60 151M = 167514 485 AGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATCAGGAGCTGGTC FF:FFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF:F:FFFFFFFFFF:FFFF:FFFFFFFFF,FF:F::,FFFFFF:FFFFF:FFFF,FF NM:i:1 MD:Z:63T87 MC:Z:151M AS:i:146 XS:i:116 A00126:224:H7HMWDSX3:3:1115:11966:24846 97 Test_seq 162525 24 23M128S = 362198 199824 CTCCTGGGTAGTTAGATGGGATCACTCAGCAGGAATTATGCGGTGCATTTTTCTGTAGGTTAAGTCGTCTAGGGAGCTGAAGGATAGTCTCATTAGTTCTCAACCTCTTCTCCATACCTTTTGTGGGAGTGCACACACATGCGCCCACGTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:23 MC:Z:151M AS:i:23 XS:i:0 SA:Z:Test_seq,361994,+,23S128M,60,0; A00126:224:H7HMWDSX3:3:1115:11966:24846 2145 Test_seq 361994 60 23H128M = 362198 355 ACTCAGCAGGAATTATGCGGTGCATTTTTCTGTAGGTTAAGTCGTCTAGGGAGCTGAAGGATAGTCTCATTAGTTCTCAACCTCTTCTCCATACCTTTTGTGGGAGTGCACACACATGCGCCCACGTG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:128 MC:Z:151M AS:i:128 XS:i:0 SA:Z:Test_seq,162525,+,23M128S,24,0; A00126:224:H7HMWDSX3:3:1115:11966:24846 145 Test_seq 362198 60 151M = 162525 -199824 TTGTCTTTTTCTGTTTAAAACTATTTGTGCTTATCGTGGACAATATTGAAAGTAATAAGAAAGGGTAAAACATCCTTTGAAAGTCTGGTAAATGAGAACACACTACTGCATTTTCCTTTCCTTTTTCTTGTAAGCATAGTTTTTGTCTGTT FFF:F,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:23M128S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1115:1967:25974 97 Test_seq 167150 18 151M = 167449 450 TTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTAGCTGCCTGAATATCTTCTTTAGCGAAATGTGTGTTCATGTCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTCACAGAAT FFFFFFFFFFFFFF:F,,FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFF:FFF,FFF:FF,FFFFFFFFFFF,FFFFFFFFF,F,,FFFF:::FFF:FFFF,FFFF,F:FFFF:F,,FFFF,FFFFFFFFF:FF:F:FFF:F NM:i:6 MD:Z:28A23G22T15A1T49G7 MC:Z:151M AS:i:121 XS:i:111 XA:Z:Test_seq,-213734,151M,8; A00126:224:H7HMWDSX3:3:1115:1967:25974 145 Test_seq 167449 60 151M = 167150 -450 TCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTG :FFF:FFF,:FFFFF:FFFFF:FFFFF,FFFFF:FFFFFF:FFFF:FFFF:FFF,FFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFF,F,FFFFF:::FFFFFFFF:F:F,FFFFFFF:FF:FFFFFFFFFFFF:FFFFFFFFFF, NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:101 A00126:224:H7HMWDSX3:3:1115:7789:25974 81 Test_seq 167797 60 151M = 167568 -380 CAGGCTGTTTTGACTACTGTAGCTGTATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:21C129 MC:Z:32S119M AS:i:146 XS:i:113 A00126:224:H7HMWDSX3:3:1115:7789:25974 161 Test_seq 167568 34 32S119M = 167797 380 CATATTTATGCCTATTACCAAGAAAGGTGATCGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGCTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F NM:i:1 MD:Z:76T42 MC:Z:151M AS:i:114 XS:i:99 XA:Z:Test_seq,-213352,108M2I9M32S,4; A00126:224:H7HMWDSX3:3:1115:18032:26678 81 Test_seq 170320 60 151M = 170112 -359 CTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTT FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:56C94 MC:Z:151M AS:i:146 XS:i:48 A00126:224:H7HMWDSX3:3:1115:18032:26678 161 Test_seq 170112 60 151M = 170320 359 TGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTA FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFF:F,:FFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFF:FFFFFFFFF:FF:FF:FFF::FFFFFFFFFFF,FFF:FF NM:i:3 MD:Z:3G20A59A66 MC:Z:151M AS:i:137 XS:i:53 A00126:224:H7HMWDSX3:3:1115:6686:27383 97 Test_seq 166310 60 151M = 166550 365 CCCAGGCGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:F,FF,F NM:i:1 MD:Z:6T144 MC:Z:26S125M AS:i:146 XS:i:27 A00126:224:H7HMWDSX3:3:1115:6686:27383 145 Test_seq 166550 60 26S125M = 166310 -365 TTATCCCGGAAACCTCTTTGCTTTTAGATCAATAAAAAACCCTCTCCCTCCCTTCCTCCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTACTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCC FFFFFFFFFFFFFFF::,FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFF:FFFFFF NM:i:3 MD:Z:27C50T9C36 MC:Z:151M AS:i:110 XS:i:63 SA:Z:Test_seq,166445,-,27M124S,48,0; A00126:224:H7HMWDSX3:3:1115:6686:27383 2193 Test_seq 166445 48 27M124H = 166310 -162 TTATCCCGGAAACCTCTTTGCTTTTAG FFFFFFFFFFFFFFF::,FFFFFFFFF NM:i:0 MD:Z:27 MC:Z:151M AS:i:27 XS:i:0 SA:Z:Test_seq,166550,-,26S125M,60,3; A00126:224:H7HMWDSX3:3:1115:27516:27915 81 Test_seq 169628 60 151M = 169316 -463 CGTTGCATTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATCTCTAGTTTTATTGCCTTGTGGTCTGA FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:8 MD:Z:0T5T5A22G83C4T5C2C17 MC:Z:151M AS:i:115 XS:i:0 A00126:224:H7HMWDSX3:3:1115:27516:27915 161 Test_seq 169316 60 151M = 169628 463 TAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATGTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGG FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,F:FFFFF,FFFF,FFFF::FFFFFF,FFF,FF NM:i:1 MD:Z:37T113 MC:Z:151M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1115:31584:28040 81 Test_seq 168218 60 128M23S = 167925 -421 TCTTTTTGTTGATGTAGAGGAATCCAGGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTACTTTCAGTAGTTCTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCAATCATTTCTTCATTTGCTC :FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:4 MD:Z:26A26T27G11T34 MC:Z:151M AS:i:108 XS:i:31 A00126:224:H7HMWDSX3:3:1115:31584:28040 161 Test_seq 167925 60 151M = 168218 421 AGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF,FFFFFFFFFFFFFFFFF:FF NM:i:1 MD:Z:135C15 MC:Z:128M23S AS:i:146 XS:i:73 A00126:224:H7HMWDSX3:3:1115:15944:29731 97 Test_seq 166867 38 151M = 167169 453 GCTGCAATAAACATGGGTGTGCATATATCTGTTTGTATGAAGGCTCTTGTATCTCTAGGGTCTATTCCTAGGAGTGGGATTTCTGGGTTGTATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATGGATTTCCAAAGTGGTTGTA FFFFF,,FFFFFF:FFF:FFFFF:FFFFFFFF,FFF:FFF:FFF:F::FFFFFFF::FFF,,::FFFFFFFFFFFFFF:,::F:FFF,FFF,,FF,,FFFFF,FFFFFFFFFFF,FFFFFFF,F:FF:FFFFFFF:FFF::FFFFFFFFF, NM:i:5 MD:Z:26G9G13T10A8T80 MC:Z:151M AS:i:126 XS:i:106 XA:Z:Test_seq,-214017,151M,9; A00126:224:H7HMWDSX3:3:1115:15944:29731 145 Test_seq 167169 52 151M = 166867 -453 TAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATC :FF,FF,F:FFF:FFFF,FFFFF,FF:FF:FFFFFFFFFF::FF:FFF:FFF:F,FFFFFF,F:FFFFFFF:FFFFFFFFFFFFF,FFFFFF:FFFF:FFF,FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFF:,FFFFFFFF:FFFFFFF NM:i:1 MD:Z:74T76 MC:Z:151M AS:i:146 XS:i:121 XA:Z:Test_seq,+213715,151M,6; A00126:224:H7HMWDSX3:3:1115:19054:30639 97 Test_seq 168458 60 151M = 168590 258 ATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:37T113 MC:Z:126M25S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1115:19054:30639 145 Test_seq 168590 60 126M25S = 168458 -258 CTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATGTGGTTTTTGTCTTTTGTTTTATTTATGTGATGGATTACATTAGATCTGCTGTGAGTAGCCAA FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:3 MD:Z:46A26T23A28 MC:Z:151M AS:i:111 XS:i:81 A00126:224:H7HMWDSX3:3:1115:17933:33301 97 Test_seq 171467 60 29S122M = 171716 400 AATGAAGAAACCTTAAGAATCATGTGGGACTTAGGGTTTCTTCATTTTTTTAAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCAGCTCCTCTG FFF:FFF:FFFFFFF:F,F,FFFFFFF,FF,:FF::F::FFFFF,:FF:,FF:FF::,,FF:,F::FFFFFF::F,F::FF::FFF,F,FFF:FF,,F,,FF,F,,:F,FFFFFF:F:F,FFF:,F,,FFF,,,,FFFFFF,:FF,,,F:, NM:i:3 MD:Z:22T89T3T5 MC:Z:151M AS:i:107 XS:i:43 A00126:224:H7HMWDSX3:3:1115:17933:33301 145 Test_seq 171716 60 151M = 171467 -400 TCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTTTTCTGCAGTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATT FFF:FFFFFFFFFFFFFFFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:1T149 MC:Z:29S122M AS:i:149 XS:i:30 A00126:224:H7HMWDSX3:3:1115:4517:33583 81 Test_seq 116724 60 127M24S = 116372 -479 CTCTGCTTCCAGCCAAGATCCATCTGACATCAGCAATGATATGCCTTGTTCCACTTCCTCTTCTGAATCTGGCTTGGATTTCTGGCAGTTCCCTGTCAGTGTACTGCTGCAACATTTTTGAATGATCGATCCACAAATCGGCATATCATAG FFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,F,F:FFFFFFFFFFFFFF NM:i:1 MD:Z:19T107 MC:Z:151M AS:i:122 XS:i:45 A00126:224:H7HMWDSX3:3:1115:4517:33583 161 Test_seq 116372 60 151M = 116724 479 GCTTACATTTGTAAGAATATAGTAGTAAGTAGGAGAGAGAGAACAGCACACCTGATGCTGAGCTCTTCTTCATGTAGTCCAGCTTCTTGGAGTATTTGCTCAGTGTACAAATTGACTAAGTATGGTGAAAGGATACAACCCTGACACACAC FFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFF:F:FFFFFFFFFF:FFFFFFFF,FFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FF,FF:FFFFFF,F:FFFFF,FFFFFFFFFFFFFFFF:FFF:FFFFFFF NM:i:1 MD:Z:7C143 MC:Z:127M24S AS:i:146 XS:i:42 A00126:224:H7HMWDSX3:3:1115:31313:33771 81 Test_seq 38766 60 124M27S = 38503 -387 AACTGCTACAATCAACCAAATTGACCTCATAGACTTATACAGAACACTCCACCCAACAGCTGCAAAGTATAAGTTTTTTCTAGTACACATGGAACATTCTCTAGAATAGATCACATATTAGATCGATCAACCTCCACCACTTCATTCTGAA ,F:FFF,F:F:FF:FFF:,F,FFFF:FF,FFFFFF:F,FFFFFFF:FFFFFFF:,,::FFFF:,,,FFFF:FFFFFFFFFFFFFFF:FF,FFF,F::FFF:FFFFFF:FFFFF:FFFFFFF:FFFFFFF,FFFFF:FF,FF:FFFFFFFFF NM:i:0 MD:Z:124 MC:Z:151M AS:i:124 XS:i:29 A00126:224:H7HMWDSX3:3:1115:31313:33771 161 Test_seq 38503 60 151M = 38766 387 AATTTCTGACAGAATAGACTTTAAAGTTAAATCCACCACAAAGGATAAAGAAGGACACTACATAATGATTAAAGGGACAACTGGTCAGGAAGATATAACCATATTAAGTATTTATGCACCCAATGACAGGGCTGCAAGATTCATAAAACAA F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFF:F:FF:FF:FFFF,FFFFF:FFF,F:FF:FF,FF:FF:FFFFFFFFFF::FFFFFFFFFFFFFFFFFFF:FF::FF:FF: NM:i:0 MD:Z:151 MC:Z:124M27S AS:i:151 XS:i:31 A00126:224:H7HMWDSX3:3:1115:23113:33974 81 Test_seq 172038 60 4S71M1I63M12S = 171813 -359 TTCCGTCTCTAAGTCTGTGTTTGTTGTTCAGAGTTCGTAGATTGTTATGTATGTGATCGATTCACTTGTTTTTCCTGAGTCTTTGTTGCAAGAGGGATCCGCGGTAGCGTCCACCTAGTCCGCCATCTTGGCCCCGCCTACAGTACTAGCA FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF NM:i:3 MD:Z:44A74G14 MC:Z:151M AS:i:117 XS:i:0 A00126:224:H7HMWDSX3:3:1115:23113:33974 161 Test_seq 171813 60 151M = 172038 359 ATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATACGGACTGCTGTCTCCGAGCCATCACTGGGAAACTAGATTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:111T39 MC:Z:4S71M1I63M12S AS:i:146 XS:i:40 A00126:224:H7HMWDSX3:3:1115:12762:34491 97 Test_seq 380554 24 23M128S = 224090 -156315 TTGGTTAGCAGCTTGAGCTCTTAGATCCTTTTTATTTCAGTTGGGTCTCTAGTAATGGCCCCCATTTCATTTCTTATTTGGGTTGGTTGTGTCCTCTACTTTTTTCTTTTATCAGTTTTGTCAGTGGTTTGTTGTTTTTGTTGATCCTTTC FFFF,F,FF:FF:,,FF:F,::F::FF:FFFFF:FF,FF:F:,F:FFF:F,F:FFF,,F:FF,:,F,F:::F::F:FF,:,,:F,,FF:F:F,,,,F,,,F:F,::F,,FFF,,F,FFFF::F,F,,,::FF,,,FFFFFF,,FF,,F,:F NM:i:0 MD:Z:23 MC:Z:151M AS:i:23 XS:i:0 SA:Z:Test_seq,223789,+,23S128M,60,5; A00126:224:H7HMWDSX3:3:1115:12762:34491 2145 Test_seq 223789 60 23H128M = 224090 452 GATCCTTTTTATTTCAGTTGGGTCTCTAGTAATGGCCCCCATTTCATTTCTTATTTGGGTTGGTTGTGTCCTCTACTTTTTTCTTTTATCAGTTTTGTCAGTGGTTTGTTGTTTTTGTTGATCCTTTC ::FF:FFFFF:FF,FF:F:,F:FFF:F,F:FFF,,F:FF,:,F,F:::F::F:FF,:,,:F,,FF:F:F,,,,F,,,F:F,::F,,FFF,,F,FFFF::F,F,,,::FF,,,FFFFFF,,FF,,F,:F NM:i:5 MD:Z:27T33A0T11C36A16 MC:Z:151M AS:i:103 XS:i:0 SA:Z:Test_seq,380554,+,23M128S,24,0; A00126:224:H7HMWDSX3:3:1115:12762:34491 145 Test_seq 224090 60 151M = 380554 156315 TGTATGCATCTATTGTTGTAAATAGACCTCTGAGCACTGCCTTTGCTGTATCCCAATGGTTTTAGTATGATGTGTTTTCATTCTCGGAATTTTTTTTATTCCATCTTTGATTTCTTTTATTACGCAGTGGTTTTCAAGCAGGGTGTTACTC FFFFFF:F:F,::F:,FFFFFFFFFF:FFFFFFFF:FFFFFFFFF:FF,FFFFF,FF,,F:FF:F::FF::FFFFFF:FFFFFF,FFFF,FFFFFF:F,,FFFFFFF:FFFFFFF:FFFFFFF::FFFFFFFFFF,:FFFFFFF:FFFFFF NM:i:0 MD:Z:151 MC:Z:23M128S AS:i:151 XS:i:37 A00126:224:H7HMWDSX3:3:1115:29568:35258 81 Test_seq 166228 60 151M = 166063 -316 TGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGTTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACCCCAGGTGCCACAATTCAAAGTGGGATGCAGAGTGTTTTCATAATAGAATTGTTTTGCCCATTGACTTA :FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:3 MD:Z:114A18A8G8 MC:Z:151M AS:i:136 XS:i:76 A00126:224:H7HMWDSX3:3:1115:29568:35258 161 Test_seq 166063 60 151M = 166228 316 TCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:57 A00126:224:H7HMWDSX3:3:1115:16432:35305 97 Test_seq 167861 60 151M = 168152 409 CTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTAGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAAT FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFF:F:F:FF NM:i:1 MD:Z:71T79 MC:Z:118M33S AS:i:146 XS:i:52 A00126:224:H7HMWDSX3:3:1115:16432:35305 145 Test_seq 168152 8 118M33S = 167861 -409 CTAAGTATTTTATCTTCTTGGGGGCTACTGTGAATGGTATTGATTTGGTTATGTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATATCCTGAGACTACTCATGCCCAAAGAATGCCGAG FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,F,FFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF NM:i:2 MD:Z:17C34T65 MC:Z:151M AS:i:108 XS:i:103 XA:Z:Test_seq,+212759,23S128M,5; A00126:224:H7HMWDSX3:3:1115:2672:36401 97 Test_seq 169299 60 151M = 169513 365 CCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTT :FFFFF,,,FFFFF,FFFFFFFFFFFFFF:FFFF,:FFFFF:FFFFFFFFFFFFFFFFFFFF::FF:,F::FFFF,,,FFFFFF::FF,F:F:F,FF:FFFFF:FFFF,F:FF,FF,FFFF,FFF,,:,F:FF,FF:FF:FFFFFF:FFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:101 A00126:224:H7HMWDSX3:3:1115:2672:36401 145 Test_seq 169513 60 151M = 169299 -365 TCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATATAAATTGGCCTCTGAGCACTGCCTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGA FFFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFF::FFFFF:FFF:FFFFFFF,FFF::FFF,FFFFF,FFFFFFFFF:FFFF:FF::F:FFFF,::FFFF:FFFFFF::F:FFFFFFFF,FFF:FFFFFFFFFFFFFF:F,F,,:F NM:i:5 MD:Z:6C0C63T43T34G0 MC:Z:151M AS:i:132 XS:i:33 A00126:224:H7HMWDSX3:3:1116:21097:1329 65 Test_seq 167966 21 151M = 212718 44753 TTTGGATCGGAATTGCATTGTATGTATAGATGGCTTTTGGTAGAATAGACATTTTGACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTACTAGAGCTTTGTAGTTTTCTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF NM:i:11 MD:Z:7T4G3G2A35T29G8C34G0C4T3A11 MC:Z:151M AS:i:96 XS:i:83 XA:Z:Test_seq,-212922,151M,14; A00126:224:H7HMWDSX3:3:1116:21097:1329 129 Test_seq 212718 31 151M = 167966 -44753 TCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCCCCCA FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:108A42 MC:Z:151M AS:i:146 XS:i:131 XA:Z:Test_seq,-168170,151M,4; A00126:224:H7HMWDSX3:3:1116:9525:1548 81 Test_seq 166049 60 151M = 165792 -408 ATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTACTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:1 MD:Z:53G97 MC:Z:110M2D41M AS:i:146 XS:i:50 A00126:224:H7HMWDSX3:3:1116:9525:1548 161 Test_seq 165792 60 110M2D41M = 166049 408 CCGTCTTCCCTCTCTCTCCATCTTCCCATCCCTCCTCCAGTCAAGAGTTGCCAACACACTCTCCCGTGTCCACCTGATTTAATTAGCTCACTCTTCCTCAGCATCTCTCTCCCCCCCACTGACCAGTCCTTTTCATGCCTGATGAGTTGTC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:12 MD:Z:2A5T9T13C31A10A8G11A13^CC7G19T13 MC:Z:151M AS:i:95 XS:i:0 A00126:224:H7HMWDSX3:3:1116:28917:2190 97 Test_seq 74270 60 151M = 74429 310 TGGGAGTCCAGGGAAGCCTTCTGGAGACCAAATCTGTTACCATCCGACTCATAGTGACCCTATAGGACAGAATAGAACTCCCCCATAGGGTTTCCAAGGCTGTAAACCTTTACAGAAGCAGATTGCCATGTCTTTCTCCCACGAAGCAGCT FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:41 A00126:224:H7HMWDSX3:3:1116:28917:2190 145 Test_seq 74429 60 151M = 74270 -310 CGAACCGCGGACCTTTCAGTCAGCAGCCGAGTGCTTCACCACCGTACCACCAGGGCTCCCCTCCTGCGGAAAGTGATGCATAAACTGAAATCTGACCAATACATAGACTCAGCCCCCAGGATAAGAGGAACAAGAGCAGGAGTGTTCTAGG FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1116:3495:3724 113 Test_seq 436621 60 151M = 335669 -101072 AAAAATGCCACGTTGGATTAACCTTGACTCAACTGGACATGTAGATACCCCATGTGGATGGTTAGAGGGGTGGCAAGGTCCATCCTCCCCAAAGAGACTGTATCCCTACCTGCCCGAGGACCTGAAGTGGTTACGTGTCCAACCAGTTGCC FFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFF NM:i:0 MD:Z:151 MC:Z:119S32M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1116:3495:3724 177 Test_seq 335669 60 119S32M = 436621 101072 AAACTGGTGTTCAAATGTGTTTTACCTTTTGGAAGAAAAATATGATTTTCTAAAGGAGGAAAATAAGAAAAAGTATAGATTTTTCATAGTTTCACTTTTCACAATGTGGAAAAAAGATCGATCTATCCTGAAGTACCTTAAGGTTAGTGGA FF:FF:FFFFF,FFFFFFFFFFFFF:FFFFF,FFFF,FF:FFFF,FFF:FFF:FF:F,FFF:FFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:32 MC:Z:151M AS:i:32 XS:i:0 SA:Z:Test_seq,436401,+,32S119M,60,0; A00126:224:H7HMWDSX3:3:1116:3495:3724 2209 Test_seq 436401 60 32H119M = 436621 371 GATCTTTTTTCCACATTGTGAAAAGTGAAACTATGAAAAATCTATACTTTTTCTTATTTTCCTCCTTTAGAAAATCATATTTTTCTTCCAAAAGGTAAAACACATTTGAACACCAGTTT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:FFF,F:FF:FFF:FFF,FFFF:FF,FFFF,FFFFF:FFFFFFFFFFFFF,FFFFF:FF:FF NM:i:0 MD:Z:119 MC:Z:151M AS:i:119 XS:i:0 SA:Z:Test_seq,335669,-,119S32M,60,0; A00126:224:H7HMWDSX3:3:1116:1886:5885 97 Test_seq 133235 60 151M = 133507 423 AATCATGGTCCAGCCAAGTTGGCACATAAGGTTAACAATCATAGCCAGCGTTACTCTTGCCTTGCACCTCGGTGTTCGTTACTGCCAGATGTACGTCGTTAATGTGCGTAACAGTCAGATACGAGATTTTTTACTATTGTCATGAACGTAA FFFFFFF:FFFF,FFFFFFF,FFFFF:FF,FFFF::,FFFFFFFFFF,F:FFFFFFFFF,FFFFFF,FFF,FFFFFF,FFFFF,FFFFFFFFFF:FFFFFFFFFF:::FFF,FFF:F,FFFF,F:FFFFFF:F,FFFF,F,FF::F,FFFF NM:i:1 MD:Z:36C114 MC:Z:151M AS:i:146 XS:i:0 A00126:224:H7HMWDSX3:3:1116:1886:5885 145 Test_seq 133507 60 151M = 133235 -423 GGGGTTTCACTGTCTGGTTTTTAGAAGCAGCATGCCAGGTCTCTGTTCCCTAGTCCACCTTAGTCTGGACACTCCGCTGAAACCTGTCCAGTGTCATAGCGACACACAGGCCTACACTGAGAGACAAGTGGTGGATGAATTTGAAGTACGT :F::FFFFFFFF,FF,FFF,,F:FFF,FFFFFF,FFF,:F:F::FFFF,FFF:F,:FFFFFFFF:,,F,:FF:FFFFFFFFFFFFFF:FFFF:F:FFFFF::FFFFFFFFF,F,FFFFFFFFFF,FFFFFFFFF,FF,F:FFFFFFFFF,F NM:i:5 MD:Z:12G29T70C20C2C13 MC:Z:151M AS:i:126 XS:i:0 A00126:224:H7HMWDSX3:3:1116:5041:6715 97 Test_seq 168462 60 151M = 168674 349 GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGA FFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFF::F:F:F,FFFFFFFFFFFF:F:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFF:FFFFFF:FF:FFFF,FFFFFFFF:FFF,F:,FFFFF:FFF NM:i:1 MD:Z:33T117 MC:Z:137M14S AS:i:146 XS:i:91 A00126:224:H7HMWDSX3:3:1116:5041:6715 145 Test_seq 168674 60 137M14S = 168462 -349 TCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTCTTTTGATATTGAACCAGCCTTGCATACCTGATATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTGATCTCCGGGACTT F:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:F:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:5 MD:Z:42T10T2C7A19G52 MC:Z:151M AS:i:112 XS:i:30 A00126:224:H7HMWDSX3:3:1116:31720:7545 65 Test_seq 305328 6 20M131S = 303522 -1807 CCATTGCTATCAAGTGGATTGATCACTCCCTGGAGAAGGACATCATGCTTGGTAAAGTAGAGGGCCAACATGGATTGACACAGTGGCTGCAACAATGGGCTCAAGCATAACAACAACTGTGAGGATGGCGCAGGGCTGGGCAGTGTTTCAT FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFF:FF:FFF:FFFFF:F:FFFFFFFFF,F::FFFFFF:FFFFFFFFFFFFFFF,F:FFFFF:FFFFFFF:FFFFFFFFFFFFFFFFF NM:i:0 MD:Z:20 MC:Z:151M AS:i:20 XS:i:0 SA:Z:Test_seq,303836,-,131M20S,60,0; A00126:224:H7HMWDSX3:3:1116:31720:7545 2129 Test_seq 303836 60 131M20H = 303522 -445 ATGAAACACTGCCCAGCCCTGCGCCATCCTCACAGTTGTTGTTATGCTTGAGCCCATTGTTGCAGCCACTGTGTCAATCCATGTTGGCCCTCTACTTTACCAAGCATGATGTCCTTCTCCAGGGAGTGATC FFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:F,FFFFFFFFFFFFFFF:FFFFFF::F,FFFFFFFFF:F:FFFFF:FFF:FF:FFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:131 MC:Z:151M AS:i:131 XS:i:43 SA:Z:Test_seq,305328,+,20M131S,6,0; A00126:224:H7HMWDSX3:3:1116:31720:7545 129 Test_seq 303522 60 151M = 305328 1807 ATCTTTTATTTGGGAGAAACAAGTCAGAATTAATTCCTGTTGATTGCACTGAATTATAAATGATGAAGAATCAGAAATCAGCTACTGGCCCACAGGCATGTTGCAGAAACTCAATAAATGTTAGCCATCATCTCCTTTTTCATCATCATAA FFFFFFF:FFFF:F:FFFFFFFFFF:FFF:FFFF:FFFF,FFFFFFFFF,FFFFF::FFF:FFFFFFFFFFFFFFF,F:FFFFFFFFFF:FF:FF:FFFF,FFF:FFF:FFFFFF:FFFFFF,F:F,FFFFF:FFF:FFF:FFFF,FFF:F NM:i:0 MD:Z:151 MC:Z:20M131S AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1116:30870:8296 97 Test_seq 169295 60 151M = 169582 438 GTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTG FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFF:FFFFFFF:FFF:F:FFFFFFFF:F:F:FFFFFFFFFFF:FFFFFFF,FFFFF:FFFF:F,FFFFFFFFFFFF NM:i:1 MD:Z:14T136 MC:Z:151M AS:i:146 XS:i:107 A00126:224:H7HMWDSX3:3:1116:30870:8296 145 Test_seq 169582 60 151M = 169295 -438 GCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCC :FFF:FFFFFFFFF,:FFF,FFFFF:FFFFFFF:FFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF: NM:i:3 MD:Z:46T11A22G69 MC:Z:151M AS:i:136 XS:i:22 A00126:224:H7HMWDSX3:3:1116:5186:8719 81 Test_seq 169618 60 129M22S = 169344 -403 TTTTCATTCTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTCTTCTGTTATTGATCGATCTGTCATACTCTG F:FFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFF:FFF:FFFFFFFFFFF NM:i:5 MD:Z:10T11A22G70A3T8 MC:Z:151M AS:i:104 XS:i:0 A00126:224:H7HMWDSX3:3:1116:5186:8719 161 Test_seq 169344 60 151M = 169618 403 TTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGT FFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFF,FFFFF:FFFF:FFFF,FF:FFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFF,FFFFFFFF,FFF:FFFFFF,FFFFFFFFFFFFF:FFFFFFF,FFFF,F:FFFFF,FFFF,F:F, NM:i:1 MD:Z:123G27 MC:Z:129M22S AS:i:146 XS:i:101 A00126:224:H7HMWDSX3:3:1116:11080:9690 97 Test_seq 171531 60 151M = 171767 387 TTCCCTGGTCCTCCAGAAGTCCTAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGTGTTGTCAAATTCTATAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGTATTCTTGCAACTTATTAAT FFFFFFF:F,FFFFFFFFFF::FFFFFFFFFF:FFFFFFF:FF:F:F,FFFFFFFF:F:::,FFFF:FFFFFFFFFFF,FF:FFF:F,:FFFFFFF:,FFFFFFF::FFF:FF:F,:F,,F:::F,:FFFFF,FF,FFF,,F,:FFFFF:F NM:i:6 MD:Z:22C29T16C6T6G47G19 MC:Z:151M AS:i:121 XS:i:53 A00126:224:H7HMWDSX3:3:1116:11080:9690 145 Test_seq 171767 60 151M = 171531 -387 ATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF,F:FFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:151 MC:Z:151M AS:i:151 XS:i:0 A00126:224:H7HMWDSX3:3:1116:31367:10222 97 Test_seq 168346 60 130M = 168472 256 ATCATGTCATCTGCAAATAGTGATAGCTTTACTTTGTCCTTACCAATCTGGATACCCTTTATTTTTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGTGGTGATAAAG * NM:i:7 MD:Z:0G18A14C0C5G22C54C10 MC:Z:130M AS:i:99 XS:i:40 A00126:224:H7HMWDSX3:3:1116:31367:10222 145 Test_seq 168472 60 130M = 168346 -256 AAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA * NM:i:0 MD:Z:130 MC:Z:130M AS:i:130 XS:i:75