# HG changeset patch
# User iuc
# Date 1714478521 0
# Node ID 6cbca566120535e33ac73500849b675e60065ea3
# Parent  81fc1418fbfbe38c63bdf798ba6c6c6f38744384
planemo upload for repository https://github.com/open2c/pairtools commit 162c595b19e442f40be4c2f5434b20b56ade52d4

diff -r 81fc1418fbfb -r 6cbca5661205 macros.xml
--- a/macros.xml	Tue Apr 16 12:50:25 2024 +0000
+++ b/macros.xml	Tue Apr 30 12:02:01 2024 +0000
@@ -1,5 +1,5 @@
 <macros>
-    <token name="@TOOL_VERSION@">1.0.3</token>
+    <token name="@TOOL_VERSION@">1.1.0</token>
     <token name="@SUFFIX_VERSION@">0</token>
     <xml name="edam_ontology">
         <edam_datas>
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_dedup_pairs.pairsam
--- a/test-data/output_dedup_pairs.pairsam	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_dedup_pairs.pairsam	Tue Apr 30 12:02:01 2024 +0000
@@ -39,20 +39,20 @@
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-1.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-1.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs.pairsam output_sorted_pairs.pairsam	PP:pairtools_sort-1.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/2/1/3/dataset_21372a8a-66a4-4ab8-8a38-a1e4d87d5048.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/2/outputs/dataset_b7034ba7-f57e-4752-a341-425fdb4da81e.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-1.3	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-2CCE5976	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-2.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-2.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs.pairsam output_sorted_pairs.pairsam	PP:pairtools_sort-2.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/2/1/3/dataset_21372a8a-66a4-4ab8-8a38-a1e4d87d5048.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/2/outputs/dataset_b7034ba7-f57e-4752-a341-425fdb4da81e.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-2.3	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-3CAFD9D9	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-3.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-3.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs.pairsam output_sorted_pairs.pairsam	PP:pairtools_sort-3.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/2/1/3/dataset_21372a8a-66a4-4ab8-8a38-a1e4d87d5048.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/2/outputs/dataset_b7034ba7-f57e-4752-a341-425fdb4da81e.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-3.3	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
 #samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:samtools	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-4.4	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-4.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs.pairsam output_sorted_pairs.pairsam	PP:pairtools_sort-4.4	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/2/1/3/dataset_21372a8a-66a4-4ab8-8a38-a1e4d87d5048.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/2/outputs/dataset_b7034ba7-f57e-4752-a341-425fdb4da81e.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-4.4	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 HWI-ST560:29:B0A7LABXX:2:1101:15566:24141	chrI	3199	chrI	3399	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:2414197chrI31996015S35M=3350201CTTAGACAATAAGCTAGCTTTCAAGATATAAGATACGAAATAGGGGTTGA111442422223232232<FHIIIIBCCFHEHFHHIHCGIJIDHHJ?@FHNM:i:0MD:Z:35AS:i:35XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:24141145chrI33506050M=3199-201TAGAATCTCCCATGTCAACGGGTTTCCATACCTCCCCAGCATCATACATCGHHGFAGFJJJIHHFGD?HIGCGHGHHHFAFC<IHFACHDAFDEDADB==NM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU
 HWI-ST560:29:B0A7LABXX:2:1101:18861:21891	chrI	35431	chrI	35703	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:18861:2189197chrI354316050M=35654273GACCACGTGGTCGTTGATGCTGCCAGCAGTTGTGCAGACCTGCATTGCTG114442222322<2@FEAFHFH<CFHFFH?GH:CDHGGIGEFDG<BBH9?NM:i:1MD:Z:35G14AS:i:45XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:18861:21891145chrI356546050M=35431-273ATGTGGCTGCTTTAATTGAGCCTCTTTCTGTCACCTGGCATGCTGTTAAG>EGDD?BFIIIIGBHCJIJIHFIHFHGHGGC<2GHGHFD<HFFDDDDB@BNM:i:1MD:Z:14G35AS:i:45XS:i:0Yt:Z:UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_dedup_pairs.stats
--- a/test-data/output_dedup_pairs.stats	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_dedup_pairs.stats	Tue Apr 30 12:02:01 2024 +0000
@@ -28,6 +28,33 @@
 summary/frac_cis_40kb+	0.058823529411764705
 summary/frac_dups	0.011378002528445006
 summary/complexity_naive	34495.88583157276
+summary/dist_freq_convergence/convergence_dist	1333521
+summary/dist_freq_convergence/strands_w_max_convergence_dist	++
+summary/dist_freq_convergence/convergence_rel_diff_threshold	0.05
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/++	49
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/--	58
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/-+	65
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/+-	425
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist_all_strands	597
+summary/dist_freq_convergence/n_cis_pairs_above_convergence_dist_all_strands	0
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/++	0.08207705192629816
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/--	0.09715242881072027
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/-+	0.10887772194304858
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/+-	0.711892797319933
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_cis_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/++	0.061946902654867256
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/--	0.07332490518331226
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/-+	0.08217446270543616
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/+-	0.5372945638432364
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist_all_strands	0.754740834386852
+summary/dist_freq_convergence/frac_total_mapped_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/++	0.06265984654731457
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/--	0.0741687979539642
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/-+	0.08312020460358056
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/+-	0.5434782608695652
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist_all_strands	0.7634271099744245
+summary/dist_freq_convergence/frac_total_nodups_in_cis_above_convergence_dist_all_strands	0.0
 chrom_freq/chrIV/chrIV	83
 chrom_freq/chrVII/chrVII	55
 chrom_freq/chrXV/chrXV	53
@@ -133,143 +160,279 @@
 dist_freq/2-3/-+	0
 dist_freq/2-3/--	0
 dist_freq/2-3/++	0
-dist_freq/3-6/+-	0
-dist_freq/3-6/-+	1
-dist_freq/3-6/--	0
-dist_freq/3-6/++	1
-dist_freq/6-10/+-	0
-dist_freq/6-10/-+	0
-dist_freq/6-10/--	0
-dist_freq/6-10/++	0
-dist_freq/10-18/+-	0
-dist_freq/10-18/-+	0
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-dist_freq/32-56/+-	0
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-dist_freq/32-56/--	0
-dist_freq/32-56/++	0
-dist_freq/56-100/+-	0
-dist_freq/56-100/-+	1
-dist_freq/56-100/--	0
-dist_freq/56-100/++	0
-dist_freq/100-178/+-	7
-dist_freq/100-178/-+	2
-dist_freq/100-178/--	2
-dist_freq/100-178/++	0
-dist_freq/178-316/+-	334
-dist_freq/178-316/-+	9
-dist_freq/178-316/--	1
-dist_freq/178-316/++	0
-dist_freq/316-562/+-	34
-dist_freq/316-562/-+	7
-dist_freq/316-562/--	0
-dist_freq/316-562/++	0
-dist_freq/562-1000/+-	1
-dist_freq/562-1000/-+	11
-dist_freq/562-1000/--	0
-dist_freq/562-1000/++	1
-dist_freq/1000-1778/+-	0
-dist_freq/1000-1778/-+	5
-dist_freq/1000-1778/--	6
-dist_freq/1000-1778/++	1
-dist_freq/1778-3162/+-	5
-dist_freq/1778-3162/-+	7
-dist_freq/1778-3162/--	2
-dist_freq/1778-3162/++	0
-dist_freq/3162-5623/+-	4
-dist_freq/3162-5623/-+	4
-dist_freq/3162-5623/--	6
-dist_freq/3162-5623/++	4
-dist_freq/5623-10000/+-	9
-dist_freq/5623-10000/-+	4
-dist_freq/5623-10000/--	9
-dist_freq/5623-10000/++	5
-dist_freq/10000-17783/+-	7
-dist_freq/10000-17783/-+	4
-dist_freq/10000-17783/--	8
-dist_freq/10000-17783/++	10
-dist_freq/17783-31623/+-	9
-dist_freq/17783-31623/-+	4
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-dist_freq/31623-56234/-+	4
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-dist_freq/31623-56234/++	10
-dist_freq/56234-100000/+-	2
-dist_freq/56234-100000/-+	1
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-dist_freq/56234-100000/++	5
-dist_freq/100000-177828/+-	3
-dist_freq/100000-177828/-+	1
-dist_freq/100000-177828/--	1
-dist_freq/100000-177828/++	2
-dist_freq/177828-316228/+-	3
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-dist_freq/177828-316228/++	3
-dist_freq/316228-562341/+-	0
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+dist_freq/4-6/+-	0
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+dist_freq/6-7/+-	0
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+dist_freq/133-178/+-	6
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+dist_freq/178-237/+-	116
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+dist_freq/237-316/-+	6
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diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_dedup_pairs_markdups.pairsam
--- a/test-data/output_dedup_pairs_markdups.pairsam	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_dedup_pairs_markdups.pairsam	Tue Apr 30 12:02:01 2024 +0000
@@ -39,20 +39,20 @@
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-1.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-1.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-1.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-1.3	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-2CCE5976	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-2.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-2.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-2.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-2.3	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-3CAFD9D9	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-3.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-3.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-3.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-3.3	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
 #samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:samtools	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-4.4	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-4.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-4.4	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-4.4	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 HWI-ST560:29:B0A7LABXX:2:1101:15566:24141	chrI	3199	chrI	3399	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:2414197chrI31996015S35M=3350201CTTAGACAATAAGCTAGCTTTCAAGATATAAGATACGAAATAGGGGTTGA111442422223232232<FHIIIIBCCFHEHFHHIHCGIJIDHHJ?@FHNM:i:0MD:Z:35AS:i:35XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:24141145chrI33506050M=3199-201TAGAATCTCCCATGTCAACGGGTTTCCATACCTCCCCAGCATCATACATCGHHGFAGFJJJIHHFGD?HIGCGHGHHHFAFC<IHFACHDAFDEDADB==NM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU
 HWI-ST560:29:B0A7LABXX:2:1101:18861:21891	chrI	35431	chrI	35703	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:18861:2189197chrI354316050M=35654273GACCACGTGGTCGTTGATGCTGCCAGCAGTTGTGCAGACCTGCATTGCTG114442222322<2@FEAFHFH<CFHFFH?GH:CDHGGIGEFDG<BBH9?NM:i:1MD:Z:35G14AS:i:45XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:18861:21891145chrI356546050M=35431-273ATGTGGCTGCTTTAATTGAGCCTCTTTCTGTCACCTGGCATGCTGTTAAG>EGDD?BFIIIIGBHCJIJIHFIHFHGHGGC<2GHGHFD<HFFDDDDB@BNM:i:1MD:Z:14G35AS:i:45XS:i:0Yt:Z:UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_dedup_sorted.pairsam
--- a/test-data/output_dedup_sorted.pairsam	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_dedup_sorted.pairsam	Tue Apr 30 12:02:01 2024 +0000
@@ -39,24 +39,24 @@
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-1.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-1.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-1.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_sort-1.5	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmpwmd1r52h/files/c/1/c/dataset_c1c8eade-049e-4209-b8f9-a97c31df8468.dat -o /tmp/tmpwmd1r52h/job_working_directory/000/4/outputs/dataset_22fb7731-5e54-4b91-9180-5373e3456c9c.dat --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-1.4	VN:1.0.3
+#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-1.3	VN:1.1.0
+#samheader: @PG	ID:pairtools_sort-1.5	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmptyq9evah/files/c/7/1/dataset_c7168a6f-71cc-4fb2-a696-c540bb977254.dat -o /tmp/tmptyq9evah/job_working_directory/000/4/outputs/dataset_d8a3cf41-0319-4df0-bc9f-d88012565c7e.dat --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-1.4	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-2CCE5976	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-2.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-2.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-2.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_sort-2.5	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmpwmd1r52h/files/c/1/c/dataset_c1c8eade-049e-4209-b8f9-a97c31df8468.dat -o /tmp/tmpwmd1r52h/job_working_directory/000/4/outputs/dataset_22fb7731-5e54-4b91-9180-5373e3456c9c.dat --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-2.4	VN:1.0.3
+#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-2.3	VN:1.1.0
+#samheader: @PG	ID:pairtools_sort-2.5	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmptyq9evah/files/c/7/1/dataset_c7168a6f-71cc-4fb2-a696-c540bb977254.dat -o /tmp/tmptyq9evah/job_working_directory/000/4/outputs/dataset_d8a3cf41-0319-4df0-bc9f-d88012565c7e.dat --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-2.4	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-3CAFD9D9	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-3.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-3.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-3.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_sort-3.5	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmpwmd1r52h/files/c/1/c/dataset_c1c8eade-049e-4209-b8f9-a97c31df8468.dat -o /tmp/tmpwmd1r52h/job_working_directory/000/4/outputs/dataset_22fb7731-5e54-4b91-9180-5373e3456c9c.dat --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-3.4	VN:1.0.3
+#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-3.3	VN:1.1.0
+#samheader: @PG	ID:pairtools_sort-3.5	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmptyq9evah/files/c/7/1/dataset_c7168a6f-71cc-4fb2-a696-c540bb977254.dat -o /tmp/tmptyq9evah/job_working_directory/000/4/outputs/dataset_d8a3cf41-0319-4df0-bc9f-d88012565c7e.dat --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-3.4	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
 #samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:samtools	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-4.4	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-4.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-4.4	VN:1.0.2
-#samheader: @PG	ID:pairtools_sort-4.6	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmpwmd1r52h/files/c/1/c/dataset_c1c8eade-049e-4209-b8f9-a97c31df8468.dat -o /tmp/tmpwmd1r52h/job_working_directory/000/4/outputs/dataset_22fb7731-5e54-4b91-9180-5373e3456c9c.dat --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-4.5	VN:1.0.3
+#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-4.4	VN:1.1.0
+#samheader: @PG	ID:pairtools_sort-4.6	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmptyq9evah/files/c/7/1/dataset_c7168a6f-71cc-4fb2-a696-c540bb977254.dat -o /tmp/tmptyq9evah/job_working_directory/000/4/outputs/dataset_d8a3cf41-0319-4df0-bc9f-d88012565c7e.dat --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-4.5	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 HWI-ST560:29:B0A7LABXX:2:1101:15566:24141	chrI	3199	chrI	3399	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:2414197chrI31996015S35M=3350201CTTAGACAATAAGCTAGCTTTCAAGATATAAGATACGAAATAGGGGTTGA111442422223232232<FHIIIIBCCFHEHFHHIHCGIJIDHHJ?@FHNM:i:0MD:Z:35AS:i:35XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:24141145chrI33506050M=3199-201TAGAATCTCCCATGTCAACGGGTTTCCATACCTCCCCAGCATCATACATCGHHGFAGFJJJIHHFGD?HIGCGHGHHHFAFC<IHFACHDAFDEDADB==NM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU
 HWI-ST560:29:B0A7LABXX:2:1101:18861:21891	chrI	35431	chrI	35703	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:18861:2189197chrI354316050M=35654273GACCACGTGGTCGTTGATGCTGCCAGCAGTTGTGCAGACCTGCATTGCTG114442222322<2@FEAFHFH<CFHFFH?GH:CDHGGIGEFDG<BBH9?NM:i:1MD:Z:35G14AS:i:45XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:18861:21891145chrI356546050M=35431-273ATGTGGCTGCTTTAATTGAGCCTCTTTCTGTCACCTGGCATGCTGTTAAG>EGDD?BFIIIIGBHCJIJIHFIHFHGHGGC<2GHGHFD<HFFDDDDB@BNM:i:1MD:Z:14G35AS:i:45XS:i:0Yt:Z:UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_dups_pairs_markdups.pairsam
--- a/test-data/output_dups_pairs_markdups.pairsam	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_dups_pairs_markdups.pairsam	Tue Apr 30 12:02:01 2024 +0000
@@ -39,20 +39,20 @@
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-1.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-1.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --output-dups output_dups_pairs_markdups.pairsam --mark-dups output_sorted_pairs.pairsam	PP:pairtools_sort-1.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/6/8/8/dataset_688d7caa-53ad-49d2-ab0b-d5ff0c2f9a6e.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/4/outputs/dataset_3f551c40-c5f0-4546-9a95-9cff58b6eaf8.dat --output-dups /tmp/tmpp1v7bap4/job_working_directory/000/4/outputs/dataset_46064cb0-a915-4a2c-b434-30912a2c1c6e.dat --mark-dups --nproc-in 1 --nproc-out 1	PP:pairtools_sort-1.3	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-2CCE5976	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-2.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-2.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --output-dups output_dups_pairs_markdups.pairsam --mark-dups output_sorted_pairs.pairsam	PP:pairtools_sort-2.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/6/8/8/dataset_688d7caa-53ad-49d2-ab0b-d5ff0c2f9a6e.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/4/outputs/dataset_3f551c40-c5f0-4546-9a95-9cff58b6eaf8.dat --output-dups /tmp/tmpp1v7bap4/job_working_directory/000/4/outputs/dataset_46064cb0-a915-4a2c-b434-30912a2c1c6e.dat --mark-dups --nproc-in 1 --nproc-out 1	PP:pairtools_sort-2.3	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-3CAFD9D9	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-3.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-3.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --output-dups output_dups_pairs_markdups.pairsam --mark-dups output_sorted_pairs.pairsam	PP:pairtools_sort-3.3	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/6/8/8/dataset_688d7caa-53ad-49d2-ab0b-d5ff0c2f9a6e.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/4/outputs/dataset_3f551c40-c5f0-4546-9a95-9cff58b6eaf8.dat --output-dups /tmp/tmpp1v7bap4/job_working_directory/000/4/outputs/dataset_46064cb0-a915-4a2c-b434-30912a2c1c6e.dat --mark-dups --nproc-in 1 --nproc-out 1	PP:pairtools_sort-3.3	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
 #samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:samtools	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-4.4	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-4.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --output-dups output_dups_pairs_markdups.pairsam --mark-dups output_sorted_pairs.pairsam	PP:pairtools_sort-4.4	VN:1.0.2
+#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/6/8/8/dataset_688d7caa-53ad-49d2-ab0b-d5ff0c2f9a6e.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/4/outputs/dataset_3f551c40-c5f0-4546-9a95-9cff58b6eaf8.dat --output-dups /tmp/tmpp1v7bap4/job_working_directory/000/4/outputs/dataset_46064cb0-a915-4a2c-b434-30912a2c1c6e.dat --mark-dups --nproc-in 1 --nproc-out 1	PP:pairtools_sort-4.4	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 HWI-ST560:29:B0A7LABXX:2:1101:2692:4382	chrIII	80421	chrIII	80656	+	-	DD	HWI-ST560:29:B0A7LABXX:2:1101:2692:4382161chrIII804216050M=80607236CTCTTCTAGCAGGAAGAGATCGTCCTTTTTACTGTCCTCGAATCCTACCGCCCFFFFFHHHHHGIIGIGIJIIJJJJJJJJIJJIJJIJJJIJIIFIJIINM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:2692:438281chrIII806076050M=80421-236ATCTTATATGCCTAATACAAAAAGAAGCTCACTAACATCAAAATCATTATHIGGFIIGIIJJJJJIHJJJJHHFIJJIHIFIJJJHGCC>332224D=:1NM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU
 HWUSI-EAS1533_0033_FC:1:1:2403:20808	chrIV	497939	chrIV	498215	+	-	DD	HWUSI-EAS1533_0033_FC:1:1:2403:2080897chrIV4979396036M=498180277CGCTTTCATTACTCGAATCCGTCAAAGACGCTTCTT4544588888C@@CC@CCC@@@C@@@C@CCC@C@CCNM:i:1MD:Z:0A35AS:i:35XS:i:0Yt:Z:UU	HWUSI-EAS1533_0033_FC:1:1:2403:20808145chrIV4981806036M=497939-277GGCTGGTGAAGGAGTATTATTTACAATTCTAAAGCN@@C@@@@CC@CC@CC22C@@@CCCCC888886666)NM:i:1MD:Z:35T0AS:i:35XS:i:0Yt:Z:UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_pairs_split.bam
Binary file test-data/output_pairs_split.bam has changed
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_pairs_split.pairs
--- a/test-data/output_pairs_split.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_pairs_split.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -39,24 +39,24 @@
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-1.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-1.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-1.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_split-1.5	PN:pairtools_split	CL:/usr/local/bin/pairtools split /tmp/tmpnt_hb134/files/7/4/2/dataset_7420fb9f-e3dd-4326-8cef-1e023c2b5e85.dat --output-pairs /tmp/tmpnt_hb134/job_working_directory/000/2/outputs/dataset_ba891b58-22e9-49f5-83f9-063b56eda346.dat --output-sam ./output.bam --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-1.4	VN:1.0.3
+#samheader: @PG	ID:pairtools_dedup-1.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-1.3	VN:1.1.0
+#samheader: @PG	ID:pairtools_split-1.5	PN:pairtools_split	CL:/usr/local/bin/pairtools split /tmp/tmpms_dxt0a/files/8/b/9/dataset_8b97afbd-6770-4cb4-8bf1-fdc78bbce0af.dat --output-pairs /tmp/tmpms_dxt0a/job_working_directory/000/2/outputs/dataset_da90611d-b0d1-447c-ae61-f9ac7147d51d.dat --output-sam ./output.bam --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-1.4	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-2CCE5976	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-2.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-2.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-2.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_split-2.5	PN:pairtools_split	CL:/usr/local/bin/pairtools split /tmp/tmpnt_hb134/files/7/4/2/dataset_7420fb9f-e3dd-4326-8cef-1e023c2b5e85.dat --output-pairs /tmp/tmpnt_hb134/job_working_directory/000/2/outputs/dataset_ba891b58-22e9-49f5-83f9-063b56eda346.dat --output-sam ./output.bam --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-2.4	VN:1.0.3
+#samheader: @PG	ID:pairtools_dedup-2.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-2.3	VN:1.1.0
+#samheader: @PG	ID:pairtools_split-2.5	PN:pairtools_split	CL:/usr/local/bin/pairtools split /tmp/tmpms_dxt0a/files/8/b/9/dataset_8b97afbd-6770-4cb4-8bf1-fdc78bbce0af.dat --output-pairs /tmp/tmpms_dxt0a/job_working_directory/000/2/outputs/dataset_da90611d-b0d1-447c-ae61-f9ac7147d51d.dat --output-sam ./output.bam --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-2.4	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
 #samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:bwa-3CAFD9D9	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-3.3	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-3.2	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-3.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_split-3.5	PN:pairtools_split	CL:/usr/local/bin/pairtools split /tmp/tmpnt_hb134/files/7/4/2/dataset_7420fb9f-e3dd-4326-8cef-1e023c2b5e85.dat --output-pairs /tmp/tmpnt_hb134/job_working_directory/000/2/outputs/dataset_ba891b58-22e9-49f5-83f9-063b56eda346.dat --output-sam ./output.bam --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-3.4	VN:1.0.3
+#samheader: @PG	ID:pairtools_dedup-3.4	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-3.3	VN:1.1.0
+#samheader: @PG	ID:pairtools_split-3.5	PN:pairtools_split	CL:/usr/local/bin/pairtools split /tmp/tmpms_dxt0a/files/8/b/9/dataset_8b97afbd-6770-4cb4-8bf1-fdc78bbce0af.dat --output-pairs /tmp/tmpms_dxt0a/job_working_directory/000/2/outputs/dataset_da90611d-b0d1-447c-ae61-f9ac7147d51d.dat --output-sam ./output.bam --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-3.4	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
 #samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools parse -o output_parsed_pairs_bam.pairs --output-stats output_parsed_pairs.stats --min-mapq 40 --walks-policy 5unique --max-inter-align-gap 30 --chroms-path test.reduced.chrom.sizes test.bam	PP:samtools	VN:1.0.2
 #samheader: @PG	ID:pairtools_sort-4.4	PN:pairtools_sort	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools sort -o output_sorted_pairs.pairsam output_parsed_pairs_bam.pairs	PP:pairtools_parse-4.3	VN:1.0.2
-#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/scratch/ddepanis/Software/anaconda3/envs/MAP_env/bin/pairtools dedup -o output_dedup_pairs_markdups.pairsam --mark-dups --output-stats output_dedup_pairs.stats output_sorted_pairs.pairsam	PP:pairtools_sort-4.4	VN:1.0.2
-#samheader: @PG	ID:pairtools_split-4.6	PN:pairtools_split	CL:/usr/local/bin/pairtools split /tmp/tmpnt_hb134/files/7/4/2/dataset_7420fb9f-e3dd-4326-8cef-1e023c2b5e85.dat --output-pairs /tmp/tmpnt_hb134/job_working_directory/000/2/outputs/dataset_ba891b58-22e9-49f5-83f9-063b56eda346.dat --output-sam ./output.bam --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-4.5	VN:1.0.3
+#samheader: @PG	ID:pairtools_dedup-4.5	PN:pairtools_dedup	CL:/usr/local/bin/pairtools dedup /tmp/tmpp1v7bap4/files/3/7/9/dataset_379f0c04-3329-4201-9b3c-3c3a66e7eec4.dat -o /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_6f9ee270-56fb-4c2e-92f4-509340f9eb58.dat --mark-dups --output-stats /tmp/tmpp1v7bap4/job_working_directory/000/6/outputs/dataset_653a5918-ae05-4cfe-8fc9-4ecc516aeaf1.dat --nproc-in 1 --nproc-out 1	PP:pairtools_sort-4.4	VN:1.1.0
+#samheader: @PG	ID:pairtools_split-4.6	PN:pairtools_split	CL:/usr/local/bin/pairtools split /tmp/tmpms_dxt0a/files/8/b/9/dataset_8b97afbd-6770-4cb4-8bf1-fdc78bbce0af.dat --output-pairs /tmp/tmpms_dxt0a/job_working_directory/000/2/outputs/dataset_da90611d-b0d1-447c-ae61-f9ac7147d51d.dat --output-sam ./output.bam --nproc-in 1 --nproc-out 1	PP:pairtools_dedup-4.5	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type
 HWI-ST560:29:B0A7LABXX:2:1101:15566:24141	chrI	3199	chrI	3399	+	-	UU
 HWI-ST560:29:B0A7LABXX:2:1101:18861:21891	chrI	35431	chrI	35703	+	-	UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_parsed_pairs.stats
--- a/test-data/output_parsed_pairs.stats	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_parsed_pairs.stats	Tue Apr 30 12:02:01 2024 +0000
@@ -6,16 +6,14 @@
 total_nodups	308
 cis	308
 trans	0
-pair_types/MU	88
+pair_types/MU	95
 pair_types/UU	293
 pair_types/MM	49
 pair_types/UR	8
 pair_types/MR	26
 pair_types/NR	110
 pair_types/RU	7
-pair_types/nM	3
-pair_types/Mu	7
-pair_types/NM	1
+pair_types/NM	4
 cis_1kb+	15
 cis_2kb+	14
 cis_4kb+	14
@@ -31,6 +29,33 @@
 summary/frac_cis_40kb+	0.02922077922077922
 summary/frac_dups	0.0
 summary/complexity_naive	nan
+summary/dist_freq_convergence/convergence_dist	177828
+summary/dist_freq_convergence/strands_w_max_convergence_dist	++
+summary/dist_freq_convergence/convergence_rel_diff_threshold	0.05
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/++	4
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/--	2
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/-+	1
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/+-	301
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist_all_strands	308
+summary/dist_freq_convergence/n_cis_pairs_above_convergence_dist_all_strands	0
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/++	0.012987012987012988
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/--	0.006493506493506494
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/-+	0.003246753246753247
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/+-	0.9772727272727273
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_cis_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/++	0.012987012987012988
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/--	0.006493506493506494
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/-+	0.003246753246753247
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/+-	0.9772727272727273
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/++	0.012987012987012988
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/--	0.006493506493506494
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/-+	0.003246753246753247
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/+-	0.9772727272727273
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_above_convergence_dist_all_strands	0.0
 chrom_freq/Test_seq/Test_seq	308
 dist_freq/0-1/+-	0
 dist_freq/0-1/-+	0
@@ -44,143 +69,279 @@
 dist_freq/2-3/-+	0
 dist_freq/2-3/--	0
 dist_freq/2-3/++	0
-dist_freq/3-6/+-	0
-dist_freq/3-6/-+	0
-dist_freq/3-6/--	0
-dist_freq/3-6/++	0
-dist_freq/6-10/+-	0
-dist_freq/6-10/-+	0
-dist_freq/6-10/--	0
-dist_freq/6-10/++	0
-dist_freq/10-18/+-	0
-dist_freq/10-18/-+	0
-dist_freq/10-18/--	0
-dist_freq/10-18/++	0
-dist_freq/18-32/+-	0
-dist_freq/18-32/-+	0
-dist_freq/18-32/--	0
-dist_freq/18-32/++	0
-dist_freq/32-56/+-	0
-dist_freq/32-56/-+	0
-dist_freq/32-56/--	0
-dist_freq/32-56/++	0
-dist_freq/56-100/+-	1
-dist_freq/56-100/-+	0
-dist_freq/56-100/--	0
-dist_freq/56-100/++	0
-dist_freq/100-178/+-	3
-dist_freq/100-178/-+	0
-dist_freq/100-178/--	0
-dist_freq/100-178/++	0
-dist_freq/178-316/+-	53
-dist_freq/178-316/-+	0
-dist_freq/178-316/--	0
-dist_freq/178-316/++	0
-dist_freq/316-562/+-	236
-dist_freq/316-562/-+	0
-dist_freq/316-562/--	0
-dist_freq/316-562/++	0
-dist_freq/562-1000/+-	0
-dist_freq/562-1000/-+	0
-dist_freq/562-1000/--	0
-dist_freq/562-1000/++	0
-dist_freq/1000-1778/+-	1
-dist_freq/1000-1778/-+	0
-dist_freq/1000-1778/--	0
-dist_freq/1000-1778/++	0
-dist_freq/1778-3162/+-	0
-dist_freq/1778-3162/-+	0
-dist_freq/1778-3162/--	0
-dist_freq/1778-3162/++	0
-dist_freq/3162-5623/+-	0
-dist_freq/3162-5623/-+	0
-dist_freq/3162-5623/--	0
-dist_freq/3162-5623/++	0
-dist_freq/5623-10000/+-	2
-dist_freq/5623-10000/-+	0
-dist_freq/5623-10000/--	0
-dist_freq/5623-10000/++	0
-dist_freq/10000-17783/+-	1
-dist_freq/10000-17783/-+	1
-dist_freq/10000-17783/--	0
-dist_freq/10000-17783/++	0
-dist_freq/17783-31623/+-	1
-dist_freq/17783-31623/-+	0
-dist_freq/17783-31623/--	0
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-dist_freq/31623-56234/+-	0
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-dist_freq/31623-56234/--	0
-dist_freq/31623-56234/++	1
-dist_freq/56234-100000/+-	2
-dist_freq/56234-100000/-+	0
-dist_freq/56234-100000/--	1
-dist_freq/56234-100000/++	1
-dist_freq/100000-177828/+-	1
-dist_freq/100000-177828/-+	0
-dist_freq/100000-177828/--	1
-dist_freq/100000-177828/++	2
-dist_freq/177828-316228/+-	0
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-dist_freq/177828-316228/--	0
-dist_freq/177828-316228/++	0
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-dist_freq/562341-1000000/+-	0
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-dist_freq/562341-1000000/--	0
-dist_freq/562341-1000000/++	0
-dist_freq/1000000-1778279/+-	0
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-dist_freq/1000000-1778279/++	0
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diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_parsed_pairs_bam.pairs
--- a/test-data/output_parsed_pairs_bam.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_parsed_pairs_bam.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -37,14 +37,14 @@
 #samheader: @SQ	SN:chrXV	LN:1091291
 #samheader: @SQ	SN:chrXVI	LN:948066
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/c/9/dataset_5c90f563-7928-4455-84af-6129feb92ffc.dat -c /tmp/tmpvtvmbj0m/files/4/9/a/dataset_49af402e-cee9-4737-bf5e-15a04f62c1d8.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/7/outputs/dataset_461ac430-f019-4ae5-9b37-796f7c45fa35.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/e/9/dataset_be9fce34-5096-400c-9efd-1f189da7f40a.dat -c /tmp/tmpchj83off/files/1/4/3/dataset_143017f3-646f-4cc1-b3d6-fc5d73287981.dat -o /tmp/tmpchj83off/job_working_directory/000/7/outputs/dataset_3348ea1d-0460-452f-aafb-dbde21a7b812.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/c/9/dataset_5c90f563-7928-4455-84af-6129feb92ffc.dat -c /tmp/tmpvtvmbj0m/files/4/9/a/dataset_49af402e-cee9-4737-bf5e-15a04f62c1d8.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/7/outputs/dataset_461ac430-f019-4ae5-9b37-796f7c45fa35.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/e/9/dataset_be9fce34-5096-400c-9efd-1f189da7f40a.dat -c /tmp/tmpchj83off/files/1/4/3/dataset_143017f3-646f-4cc1-b3d6-fc5d73287981.dat -o /tmp/tmpchj83off/job_working_directory/000/7/outputs/dataset_3348ea1d-0460-452f-aafb-dbde21a7b812.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/c/9/dataset_5c90f563-7928-4455-84af-6129feb92ffc.dat -c /tmp/tmpvtvmbj0m/files/4/9/a/dataset_49af402e-cee9-4737-bf5e-15a04f62c1d8.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/7/outputs/dataset_461ac430-f019-4ae5-9b37-796f7c45fa35.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/e/9/dataset_be9fce34-5096-400c-9efd-1f189da7f40a.dat -c /tmp/tmpchj83off/files/1/4/3/dataset_143017f3-646f-4cc1-b3d6-fc5d73287981.dat -o /tmp/tmpchj83off/job_working_directory/000/7/outputs/dataset_3348ea1d-0460-452f-aafb-dbde21a7b812.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
-#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/c/9/dataset_5c90f563-7928-4455-84af-6129feb92ffc.dat -c /tmp/tmpvtvmbj0m/files/4/9/a/dataset_49af402e-cee9-4737-bf5e-15a04f62c1d8.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/7/outputs/dataset_461ac430-f019-4ae5-9b37-796f7c45fa35.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/e/9/dataset_be9fce34-5096-400c-9efd-1f189da7f40a.dat -c /tmp/tmpchj83off/files/1/4/3/dataset_143017f3-646f-4cc1-b3d6-fc5d73287981.dat -o /tmp/tmpchj83off/job_working_directory/000/7/outputs/dataset_3348ea1d-0460-452f-aafb-dbde21a7b812.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 HWI-ST560:29:B0A7LABXX:2:1101:5084:4188	!	0	!	0	-	-	NM	HWI-ST560:29:B0A7LABXX:2:1101:5084:4188133chrI24200*=24200GGAACCGTAAAGGTTTATCGCGGTAGTAGTTTTTCACACGATTCGATAAA?<@DFDFD=FBHHCBHGGIJIIG:C@DGD??DDH?FHGHIJJDGCGEGG;AS:i:0XS:i:0Yt:Z:NM	HWI-ST560:29:B0A7LABXX:2:1101:5084:418873chrI242009S41M=24200TTGATAGACTTCGTATGTGGAGTACTGTTTTATGGCGCTTATGTGTATTC11144222222322<<C<IHGHAEFCHEHHIHGCGHHHIIEGGGBB<<9?NM:i:0MD:Z:41AS:i:41XS:i:40XA:Z:chrXIV,-781623,50M,2;chrXI,+2426,50M,3;chrX,+9383,50M,3;chrIX,+9400,50M,3;Yt:Z:NM
 HWI-ST560:29:B0A7LABXX:2:1101:15566:24141	chrI	3199	chrI	3399	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:2414197chrI31996015S35M=3350201CTTAGACAATAAGCTAGCTTTCAAGATATAAGATACGAAATAGGGGTTGA111442422223232232<FHIIIIBCCFHEHFHHIHCGIJIDHHJ?@FHNM:i:0MD:Z:35AS:i:35XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:24141145chrI33506050M=3199-201TAGAATCTCCCATGTCAACGGGTTTCCATACCTCCCCAGCATCATACATCGHHGFAGFJJJIHHFGD?HIGCGHGHHHFAFC<IHFACHDAFDEDADB==NM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_parsed_pairs_bam_5unique.pairs
--- a/test-data/output_parsed_pairs_bam_5unique.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_parsed_pairs_bam_5unique.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -37,14 +37,14 @@
 #samheader: @SQ	SN:chrXV	LN:1091291
 #samheader: @SQ	SN:chrXVI	LN:948066
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/a/a/0/dataset_aa06559a-c31e-4f53-9ca1-50c7b65e2a6a.dat -c /tmp/tmpvtvmbj0m/files/6/0/7/dataset_6076d2d8-04ad-454f-b0d1-b44203c217ae.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/15/outputs/dataset_8206c374-986e-4a2d-883d-826510572fd0.dat --min-mapq 40 --max-molecule-size 750 --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/7/8/e/dataset_78e4da88-6bc8-43af-bfe6-8d37906fee0f.dat -c /tmp/tmpchj83off/files/5/3/5/dataset_535439dd-341d-4e21-9286-84aa079d5b25.dat -o /tmp/tmpchj83off/job_working_directory/000/15/outputs/dataset_de499407-bd0d-4497-a7ae-58bd962252d3.dat --min-mapq 40 --max-molecule-size 750 --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/a/a/0/dataset_aa06559a-c31e-4f53-9ca1-50c7b65e2a6a.dat -c /tmp/tmpvtvmbj0m/files/6/0/7/dataset_6076d2d8-04ad-454f-b0d1-b44203c217ae.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/15/outputs/dataset_8206c374-986e-4a2d-883d-826510572fd0.dat --min-mapq 40 --max-molecule-size 750 --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/7/8/e/dataset_78e4da88-6bc8-43af-bfe6-8d37906fee0f.dat -c /tmp/tmpchj83off/files/5/3/5/dataset_535439dd-341d-4e21-9286-84aa079d5b25.dat -o /tmp/tmpchj83off/job_working_directory/000/15/outputs/dataset_de499407-bd0d-4497-a7ae-58bd962252d3.dat --min-mapq 40 --max-molecule-size 750 --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/a/a/0/dataset_aa06559a-c31e-4f53-9ca1-50c7b65e2a6a.dat -c /tmp/tmpvtvmbj0m/files/6/0/7/dataset_6076d2d8-04ad-454f-b0d1-b44203c217ae.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/15/outputs/dataset_8206c374-986e-4a2d-883d-826510572fd0.dat --min-mapq 40 --max-molecule-size 750 --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/7/8/e/dataset_78e4da88-6bc8-43af-bfe6-8d37906fee0f.dat -c /tmp/tmpchj83off/files/5/3/5/dataset_535439dd-341d-4e21-9286-84aa079d5b25.dat -o /tmp/tmpchj83off/job_working_directory/000/15/outputs/dataset_de499407-bd0d-4497-a7ae-58bd962252d3.dat --min-mapq 40 --max-molecule-size 750 --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
-#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/a/a/0/dataset_aa06559a-c31e-4f53-9ca1-50c7b65e2a6a.dat -c /tmp/tmpvtvmbj0m/files/6/0/7/dataset_6076d2d8-04ad-454f-b0d1-b44203c217ae.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/15/outputs/dataset_8206c374-986e-4a2d-883d-826510572fd0.dat --min-mapq 40 --max-molecule-size 750 --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/7/8/e/dataset_78e4da88-6bc8-43af-bfe6-8d37906fee0f.dat -c /tmp/tmpchj83off/files/5/3/5/dataset_535439dd-341d-4e21-9286-84aa079d5b25.dat -o /tmp/tmpchj83off/job_working_directory/000/15/outputs/dataset_de499407-bd0d-4497-a7ae-58bd962252d3.dat --min-mapq 40 --max-molecule-size 750 --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 HWI-ST560:29:B0A7LABXX:2:1101:5084:4188	!	0	!	0	-	-	NM	HWI-ST560:29:B0A7LABXX:2:1101:5084:4188133chrI24200*=24200GGAACCGTAAAGGTTTATCGCGGTAGTAGTTTTTCACACGATTCGATAAA?<@DFDFD=FBHHCBHGGIJIIG:C@DGD??DDH?FHGHIJJDGCGEGG;AS:i:0XS:i:0Yt:Z:NM	HWI-ST560:29:B0A7LABXX:2:1101:5084:418873chrI242009S41M=24200TTGATAGACTTCGTATGTGGAGTACTGTTTTATGGCGCTTATGTGTATTC11144222222322<<C<IHGHAEFCHEHHIHGCGHHHIIEGGGBB<<9?NM:i:0MD:Z:41AS:i:41XS:i:40XA:Z:chrXIV,-781623,50M,2;chrXI,+2426,50M,3;chrX,+9383,50M,3;chrIX,+9400,50M,3;Yt:Z:NM
 HWI-ST560:29:B0A7LABXX:2:1101:15566:24141	chrI	3199	chrI	3399	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:2414197chrI31996015S35M=3350201CTTAGACAATAAGCTAGCTTTCAAGATATAAGATACGAAATAGGGGTTGA111442422223232232<FHIIIIBCCFHEHFHHIHCGIJIDHHJ?@FHNM:i:0MD:Z:35AS:i:35XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:24141145chrI33506050M=3199-201TAGAATCTCCCATGTCAACGGGTTTCCATACCTCCCCAGCATCATACATCGHHGFAGFJJJIHHFGD?HIGCGHGHHHFAFC<IHFACHDAFDEDADB==NM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_parsed_pairs_bam_min_mapq_40.pairs
--- a/test-data/output_parsed_pairs_bam_min_mapq_40.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_parsed_pairs_bam_min_mapq_40.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -37,14 +37,14 @@
 #samheader: @SQ	SN:chrXV	LN:1091291
 #samheader: @SQ	SN:chrXVI	LN:948066
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/0/f/dataset_50f1299b-3b0f-4a0a-ae1e-e09b66e47369.dat -c /tmp/tmpvtvmbj0m/files/7/5/c/dataset_75c85deb-b408-42ff-90f5-5cea61a9594f.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/11/outputs/dataset_04ba0223-4885-4724-847b-02525a7ef606.dat --min-mapq 40 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/0/b/d/dataset_0bd6cbee-0544-44c1-ad81-dd3845446ce7.dat -c /tmp/tmpchj83off/files/2/2/2/dataset_222eb307-af6a-471a-9d1d-00eab6969a45.dat -o /tmp/tmpchj83off/job_working_directory/000/11/outputs/dataset_55fc9726-73b7-4f53-83da-da08e1fbe4b9.dat --min-mapq 40 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/0/f/dataset_50f1299b-3b0f-4a0a-ae1e-e09b66e47369.dat -c /tmp/tmpvtvmbj0m/files/7/5/c/dataset_75c85deb-b408-42ff-90f5-5cea61a9594f.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/11/outputs/dataset_04ba0223-4885-4724-847b-02525a7ef606.dat --min-mapq 40 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/0/b/d/dataset_0bd6cbee-0544-44c1-ad81-dd3845446ce7.dat -c /tmp/tmpchj83off/files/2/2/2/dataset_222eb307-af6a-471a-9d1d-00eab6969a45.dat -o /tmp/tmpchj83off/job_working_directory/000/11/outputs/dataset_55fc9726-73b7-4f53-83da-da08e1fbe4b9.dat --min-mapq 40 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/0/f/dataset_50f1299b-3b0f-4a0a-ae1e-e09b66e47369.dat -c /tmp/tmpvtvmbj0m/files/7/5/c/dataset_75c85deb-b408-42ff-90f5-5cea61a9594f.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/11/outputs/dataset_04ba0223-4885-4724-847b-02525a7ef606.dat --min-mapq 40 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/0/b/d/dataset_0bd6cbee-0544-44c1-ad81-dd3845446ce7.dat -c /tmp/tmpchj83off/files/2/2/2/dataset_222eb307-af6a-471a-9d1d-00eab6969a45.dat -o /tmp/tmpchj83off/job_working_directory/000/11/outputs/dataset_55fc9726-73b7-4f53-83da-da08e1fbe4b9.dat --min-mapq 40 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
-#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/0/f/dataset_50f1299b-3b0f-4a0a-ae1e-e09b66e47369.dat -c /tmp/tmpvtvmbj0m/files/7/5/c/dataset_75c85deb-b408-42ff-90f5-5cea61a9594f.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/11/outputs/dataset_04ba0223-4885-4724-847b-02525a7ef606.dat --min-mapq 40 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/0/b/d/dataset_0bd6cbee-0544-44c1-ad81-dd3845446ce7.dat -c /tmp/tmpchj83off/files/2/2/2/dataset_222eb307-af6a-471a-9d1d-00eab6969a45.dat -o /tmp/tmpchj83off/job_working_directory/000/11/outputs/dataset_55fc9726-73b7-4f53-83da-da08e1fbe4b9.dat --min-mapq 40 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 HWI-ST560:29:B0A7LABXX:2:1101:5084:4188	!	0	!	0	-	-	NM	HWI-ST560:29:B0A7LABXX:2:1101:5084:4188133chrI24200*=24200GGAACCGTAAAGGTTTATCGCGGTAGTAGTTTTTCACACGATTCGATAAA?<@DFDFD=FBHHCBHGGIJIIG:C@DGD??DDH?FHGHIJJDGCGEGG;AS:i:0XS:i:0Yt:Z:NM	HWI-ST560:29:B0A7LABXX:2:1101:5084:418873chrI242009S41M=24200TTGATAGACTTCGTATGTGGAGTACTGTTTTATGGCGCTTATGTGTATTC11144222222322<<C<IHGHAEFCHEHHIHGCGHHHIIEGGGBB<<9?NM:i:0MD:Z:41AS:i:41XS:i:40XA:Z:chrXIV,-781623,50M,2;chrXI,+2426,50M,3;chrX,+9383,50M,3;chrIX,+9400,50M,3;Yt:Z:NM
 HWI-ST560:29:B0A7LABXX:2:1101:15566:24141	chrI	3199	chrI	3399	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:2414197chrI31996015S35M=3350201CTTAGACAATAAGCTAGCTTTCAAGATATAAGATACGAAATAGGGGTTGA111442422223232232<FHIIIIBCCFHEHFHHIHCGIJIDHHJ?@FHNM:i:0MD:Z:35AS:i:35XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:24141145chrI33506050M=3199-201TAGAATCTCCCATGTCAACGGGTTTCCATACCTCCCCAGCATCATACATCGHHGFAGFJJJIHHFGD?HIGCGHGHHHFAFC<IHFACHDAFDEDADB==NM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_parsed_pairs_bam_readid_dropped.pairs
--- a/test-data/output_parsed_pairs_bam_readid_dropped.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_parsed_pairs_bam_readid_dropped.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -37,14 +37,14 @@
 #samheader: @SQ	SN:chrXV	LN:1091291
 #samheader: @SQ	SN:chrXVI	LN:948066
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/d/5/3/dataset_d53eccb1-028d-443e-aa5b-fb883976b07e.dat -c /tmp/tmpvtvmbj0m/files/d/c/c/dataset_dcc72f04-07a7-4159-8ecd-c8b978775fa1.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/19/outputs/dataset_86720212-8079-41ce-a66f-37e5222dc3ff.dat --min-mapq 40 --max-molecule-size 750 --drop-readid --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/2/b/6/dataset_2b603561-f23a-44dd-92b4-9db918b88c03.dat -c /tmp/tmpchj83off/files/1/b/6/dataset_1b6c0c8f-df3e-40c0-9161-19d29b52573a.dat -o /tmp/tmpchj83off/job_working_directory/000/19/outputs/dataset_272af137-1fda-4769-b54c-23281e79fcd4.dat --min-mapq 40 --max-molecule-size 750 --drop-readid --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/d/5/3/dataset_d53eccb1-028d-443e-aa5b-fb883976b07e.dat -c /tmp/tmpvtvmbj0m/files/d/c/c/dataset_dcc72f04-07a7-4159-8ecd-c8b978775fa1.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/19/outputs/dataset_86720212-8079-41ce-a66f-37e5222dc3ff.dat --min-mapq 40 --max-molecule-size 750 --drop-readid --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/2/b/6/dataset_2b603561-f23a-44dd-92b4-9db918b88c03.dat -c /tmp/tmpchj83off/files/1/b/6/dataset_1b6c0c8f-df3e-40c0-9161-19d29b52573a.dat -o /tmp/tmpchj83off/job_working_directory/000/19/outputs/dataset_272af137-1fda-4769-b54c-23281e79fcd4.dat --min-mapq 40 --max-molecule-size 750 --drop-readid --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/d/5/3/dataset_d53eccb1-028d-443e-aa5b-fb883976b07e.dat -c /tmp/tmpvtvmbj0m/files/d/c/c/dataset_dcc72f04-07a7-4159-8ecd-c8b978775fa1.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/19/outputs/dataset_86720212-8079-41ce-a66f-37e5222dc3ff.dat --min-mapq 40 --max-molecule-size 750 --drop-readid --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/2/b/6/dataset_2b603561-f23a-44dd-92b4-9db918b88c03.dat -c /tmp/tmpchj83off/files/1/b/6/dataset_1b6c0c8f-df3e-40c0-9161-19d29b52573a.dat -o /tmp/tmpchj83off/job_working_directory/000/19/outputs/dataset_272af137-1fda-4769-b54c-23281e79fcd4.dat --min-mapq 40 --max-molecule-size 750 --drop-readid --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
-#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/d/5/3/dataset_d53eccb1-028d-443e-aa5b-fb883976b07e.dat -c /tmp/tmpvtvmbj0m/files/d/c/c/dataset_dcc72f04-07a7-4159-8ecd-c8b978775fa1.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/19/outputs/dataset_86720212-8079-41ce-a66f-37e5222dc3ff.dat --min-mapq 40 --max-molecule-size 750 --drop-readid --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/2/b/6/dataset_2b603561-f23a-44dd-92b4-9db918b88c03.dat -c /tmp/tmpchj83off/files/1/b/6/dataset_1b6c0c8f-df3e-40c0-9161-19d29b52573a.dat -o /tmp/tmpchj83off/job_working_directory/000/19/outputs/dataset_272af137-1fda-4769-b54c-23281e79fcd4.dat --min-mapq 40 --max-molecule-size 750 --drop-readid --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 .	!	0	!	0	-	-	NM	HWI-ST560:29:B0A7LABXX:2:1101:5084:4188133chrI24200*=24200GGAACCGTAAAGGTTTATCGCGGTAGTAGTTTTTCACACGATTCGATAAA?<@DFDFD=FBHHCBHGGIJIIG:C@DGD??DDH?FHGHIJJDGCGEGG;AS:i:0XS:i:0Yt:Z:NM	HWI-ST560:29:B0A7LABXX:2:1101:5084:418873chrI242009S41M=24200TTGATAGACTTCGTATGTGGAGTACTGTTTTATGGCGCTTATGTGTATTC11144222222322<<C<IHGHAEFCHEHHIHGCGHHHIIEGGGBB<<9?NM:i:0MD:Z:41AS:i:41XS:i:40XA:Z:chrXIV,-781623,50M,2;chrXI,+2426,50M,3;chrX,+9383,50M,3;chrIX,+9400,50M,3;Yt:Z:NM
 .	chrI	3199	chrI	3399	+	-	UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:2414197chrI31996015S35M=3350201CTTAGACAATAAGCTAGCTTTCAAGATATAAGATACGAAATAGGGGTTGA111442422223232232<FHIIIIBCCFHEHFHHIHCGIJIDHHJ?@FHNM:i:0MD:Z:35AS:i:35XS:i:0Yt:Z:UU	HWI-ST560:29:B0A7LABXX:2:1101:15566:24141145chrI33506050M=3199-201TAGAATCTCCCATGTCAACGGGTTTCCATACCTCCCCAGCATCATACATCGHHGFAGFJJJIHHFGD?HIGCGHGHHHFAFC<IHFACHDAFDEDADB==NM:i:0MD:Z:50AS:i:50XS:i:0Yt:Z:UU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_parsed_pairs_bam_readid_dropped_seq.pairs
--- a/test-data/output_parsed_pairs_bam_readid_dropped_seq.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_parsed_pairs_bam_readid_dropped_seq.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -4,13 +4,13 @@
 #chromsize: Test_seq 450060
 #samheader: @SQ	SN:Test_seq	LN:450060
 #samheader: @PG	ID:bwa-mem2	PN:bwa-mem2	VN:2.2.1	CL:bwa-mem2 mem -5SP -T0 -t 8 test test_hic_reads_1.fq test_hic_reads_2.fq
-#samheader: @PG	ID:pairtools_parse	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/6/d/e/dataset_6dee055e-91e3-4fb7-a2cb-65bb836b6b46.dat -c /tmp/tmpvtvmbj0m/files/f/6/d/dataset_f6d93a5f-aa4c-4b78-802c-e7b94fcee3e4.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/22/outputs/dataset_6d40908c-8b00-4098-8c50-3c42836de0ee.dat --min-mapq 40 --max-molecule-size 750 --drop-seq --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-mem2	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/7/b/dataset_b7b0c820-0d14-4f18-95e3-c6fb58aae265.dat -c /tmp/tmpchj83off/files/d/7/1/dataset_d718e69b-ffe9-4c9d-ab83-1d209e3a56bf.dat -o /tmp/tmpchj83off/job_working_directory/000/22/outputs/dataset_e94790d6-3c0a-4eaf-a7ee-9cab4494c297.dat --min-mapq 40 --max-molecule-size 750 --drop-seq --walks-policy 5unique --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-mem2	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 A00126:224:H7HMWDSX3:3:1101:4779:3098	!	0	Test_seq	166211	-	-	MU	A00126:224:H7HMWDSX3:3:1101:4779:3098177Test_seq214824578M=166134-48691**NM:i:3MD:Z:1T33G33A8MC:Z:78MAS:i:66XS:i:63XA:Z:Test_seq,+166134,78M,3;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1101:4779:3098113Test_seq1661344378M=21482448691**NM:i:0MD:Z:78MC:Z:78MAS:i:78XS:i:63XA:Z:Test_seq,+214829,5S73M,2;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1101:2908:3239	!	0	Test_seq	167696	-	-	MU	A00126:224:H7HMWDSX3:3:1101:2908:3239161Test_seq16733123151M=167546366**NM:i:1MD:Z:37A113MC:Z:151MAS:i:146XS:i:135XA:Z:Test_seq,-213553,151M,4;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1101:2908:323981Test_seq16754644151M=167331-366**NM:i:3MD:Z:82G15T30C21MC:Z:151MAS:i:136XS:i:114XA:Z:Test_seq,+213342,118M2I23M2I6M,8;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1101:27208:4492	Test_seq	194620	Test_seq	194909	+	-	UU	A00126:224:H7HMWDSX3:3:1101:27208:449297Test_seq19462060151M=194778290**NM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:27208:4492145Test_seq19477860132M19S=194620-290**NM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:9344:5306	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1101:9344:530697Test_seq449503760M=44950360**NM:i:4MD:Z:3T44G2C5G2MC:Z:60MAS:i:44XS:i:40XA:Z:Test_seq,+16926,60M,5;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1101:9344:5306145Test_seq4495031260M=449503-60**NM:i:1MD:Z:53T6MC:Z:60MAS:i:55XS:i:50XA:Z:Test_seq,-16926,60M,2;Yt:Z:MM
-A00126:224:H7HMWDSX3:3:1101:17607:5212	Test_seq	83425	Test_seq	93852	-	+	UR	A00126:224:H7HMWDSX3:3:1101:17607:521281Test_seq8339760122S29M=9385210428**NM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,94155,-,122M29S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1101:17607:52122129Test_seq9415560122M29H=93852-425**NM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0SA:Z:Test_seq,83397,-,122S29M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1101:17607:5212161Test_seq9385260151M=83397-10428**NM:i:0MD:Z:151MC:Z:122S29MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1101:17607:5212	Test_seq	83425	Test_seq	93852	-	+	UR	A00126:224:H7HMWDSX3:3:1101:17607:52122129Test_seq9415560122M29H=93852-425**NM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0SA:Z:Test_seq,83397,-,122S29M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1101:17607:521281Test_seq8339760122S29M=9385210428**NM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,94155,-,122M29S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1101:17607:5212161Test_seq9385260151M=83397-10428**NM:i:0MD:Z:151MC:Z:122S29MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1101:17942:7263	Test_seq	168528	Test_seq	168867	+	-	UU	A00126:224:H7HMWDSX3:3:1101:17942:726397Test_seq16852860151M=168717340**NM:i:5MD:Z:12A68A23T2A26T15MC:Z:151MAS:i:126XS:i:91Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:17942:7263145Test_seq16871760151M=168528-340**NM:i:1MD:Z:27G123MC:Z:151MAS:i:146XS:i:91Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:5683:8108	!	0	Test_seq	170834	-	+	MR	A00126:224:H7HMWDSX3:3:1101:5683:810865Test_seq137032028M123S=17083433803**NM:i:1MD:Z:7G20MC:Z:151MAS:i:23XS:i:23SA:Z:Test_seq,171170,-,119M32S,60,0;XA:Z:Test_seq,+333108,28M123S,1;Test_seq,-298750,123S26M2S,1;Test_seq,-279319,123S28M,2;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1101:5683:81082129Test_seq17117060119M32H=170834-455**NM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,137032,+,28M123S,0,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1101:5683:8108129Test_seq17083460151M=137032-33803**NM:i:0MD:Z:151MC:Z:28M123SAS:i:151XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1101:3947:9361	!	0	Test_seq	171174	-	+	NR	A00126:224:H7HMWDSX3:3:1101:3947:9361145Test_seq17134260129M22S=171174-297**NM:i:2MD:Z:58G15T54MC:Z:151MAS:i:119XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1101:3947:936197Test_seq17117460151M=171342297**NM:i:0MD:Z:151MC:Z:129M22SAS:i:151XS:i:37Yt:Z:NR
@@ -46,12 +46,12 @@
 A00126:224:H7HMWDSX3:3:1101:13042:33630	Test_seq	166363	Test_seq	166687	+	-	UU	A00126:224:H7HMWDSX3:3:1101:13042:3363097Test_seq16636360151M=166544325**NM:i:0MD:Z:151MC:Z:5M3I18M4I121MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:13042:33630145Test_seq166544605M3I18M4I121M=166363-325**NM:i:9MD:Z:59A34C49MC:Z:151MAS:i:119XS:i:62Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:29966:36072	Test_seq	170104	Test_seq	170357	+	-	UU	A00126:224:H7HMWDSX3:3:1101:29966:36072161Test_seq17010460151M=170207254**NM:i:6MD:Z:3C7G20A32A25C0A58MC:Z:151MAS:i:122XS:i:49Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:29966:3607281Test_seq17020760151M=170104-254**NM:i:1MD:Z:55A95MC:Z:151MAS:i:146XS:i:88Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:3595:36620	!	0	Test_seq	167976	-	-	MU	A00126:224:H7HMWDSX3:3:1101:3595:36620161Test_seq1675673015S136M=167826410**NM:i:4MD:Z:41G53T23T13T2MC:Z:151MAS:i:118XS:i:103XA:Z:Test_seq,-213336,124M2I10M15S,7;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1101:3595:3662081Test_seq16782660151M=167567-410**NM:i:1MD:Z:90T60MC:Z:15S136MAS:i:146XS:i:87Yt:Z:MU
-A00126:224:H7HMWDSX3:3:1102:11306:1908	Test_seq	186004	Test_seq	256018	-	-	RU	A00126:224:H7HMWDSX3:3:1102:11306:1908177Test_seq18585460151M=25599370015**NM:i:1MD:Z:31G119MC:Z:125S26MAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:11306:1908113Test_seq25599342125S26M=185854-70015**NM:i:0MD:Z:26MC:Z:151MAS:i:26XS:i:0SA:Z:Test_seq,185684,+,26S125M,60,3;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:11306:19082145Test_seq1856846026H125M=185854321**NM:i:3MD:Z:68T17G1G36MC:Z:151MAS:i:110XS:i:0SA:Z:Test_seq,255993,-,125S26M,42,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:11306:1908	Test_seq	186004	Test_seq	256018	-	-	RU	A00126:224:H7HMWDSX3:3:1102:11306:1908177Test_seq18585460151M=25599370015**NM:i:1MD:Z:31G119MC:Z:125S26MAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:11306:19082145Test_seq1856846026H125M=185854321**NM:i:3MD:Z:68T17G1G36MC:Z:151MAS:i:110XS:i:0SA:Z:Test_seq,255993,-,125S26M,42,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:11306:1908113Test_seq25599342125S26M=185854-70015**NM:i:0MD:Z:26MC:Z:151MAS:i:26XS:i:0SA:Z:Test_seq,185684,+,26S125M,60,3;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1102:7491:2785	!	0	Test_seq	168666	-	-	NR	A00126:224:H7HMWDSX3:3:1102:7491:278597Test_seq1682384324S127M=168516429**NM:i:2MD:Z:33T74G18MC:Z:151MAS:i:117XS:i:97XA:Z:Test_seq,-212674,127M24S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1102:7491:2785145Test_seq16851660151M=168238-429**NM:i:1MD:Z:79A71MC:Z:24S127MAS:i:146XS:i:82Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1102:22987:3724	!	0	Test_seq	288164	-	+	MR	A00126:224:H7HMWDSX3:3:1102:22987:3724145Test_seq27243130127S24M=28816415711**NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,288412,-,128M23S,60,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1102:22987:37242193Test_seq28841260128M23H=288164-376**NM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0SA:Z:Test_seq,272431,-,127S24M,30,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1102:22987:372497Test_seq28816460151M=272431-15711**NM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1102:22987:3724	!	0	Test_seq	288164	-	+	MR	A00126:224:H7HMWDSX3:3:1102:22987:37242193Test_seq28841260128M23H=288164-376**NM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0SA:Z:Test_seq,272431,-,127S24M,30,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1102:22987:3724145Test_seq27243130127S24M=28816415711**NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,288412,-,128M23S,60,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1102:22987:372497Test_seq28816460151M=272431-15711**NM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1102:11822:4335	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1102:11822:4335113Test_seq1661592651M=21482648668**NM:i:1MD:Z:28G22MC:Z:51MAS:i:46XS:i:38XA:Z:Test_seq,+214826,51M,3;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1102:11822:4335177Test_seq2148262851M=166159-48668**NM:i:0MD:Z:51MC:Z:51MAS:i:51XS:i:43XA:Z:Test_seq,+166159,51M,2;Yt:Z:MM
-A00126:224:H7HMWDSX3:3:1102:31313:5556	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31313:555697Test_seq15101660151M=22223571249**NM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31313:5556145Test_seq22223560121S30M=151016-71249**NM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31313:55562193Test_seq15130047121M30H=151016-405**NM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RU
-A00126:224:H7HMWDSX3:3:1102:31394:5541	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31394:554197Test_seq15101660151M=22223571249**NM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31394:5541145Test_seq22223560121S30M=151016-71249**NM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31394:55412193Test_seq15130047121M30H=151016-405**NM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:31313:5556	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31313:555697Test_seq15101660151M=22223571249**NM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31313:55562193Test_seq15130047121M30H=151016-405**NM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31313:5556145Test_seq22223560121S30M=151016-71249**NM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:31394:5541	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31394:554197Test_seq15101660151M=22223571249**NM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31394:55412193Test_seq15130047121M30H=151016-405**NM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31394:5541145Test_seq22223560121S30M=151016-71249**NM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1102:4218:5822	Test_seq	170181	Test_seq	170586	+	-	UU	A00126:224:H7HMWDSX3:3:1102:4218:5822161Test_seq17018160151M=170436406**NM:i:4MD:Z:15A78A7C1A46MC:Z:151MAS:i:131XS:i:55Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:4218:582281Test_seq17043660151M=170181-406**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:25Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:18602:6652	Test_seq	171219	Test_seq	171678	+	-	UU	A00126:224:H7HMWDSX3:3:1102:18602:6652161Test_seq17121960151M=171528460**NM:i:1MD:Z:141G9MC:Z:151MAS:i:146XS:i:79Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:18602:665281Test_seq17152860151M=171219-460**NM:i:7MD:Z:2A25T26T16C6T59T8A2MC:Z:151MAS:i:120XS:i:53Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:15619:9721	!	0	Test_seq	170377	-	-	MU	A00126:224:H7HMWDSX3:3:1102:15619:9721161Test_seq17007425151M=170227304**NM:i:7MD:Z:8A24C7G20A32A25C0A28MC:Z:151MAS:i:116XS:i:102XA:Z:Test_seq,-38508,147M4S,9;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1102:15619:972181Test_seq17022760151M=170074-304**NM:i:1MD:Z:35A115MC:Z:151MAS:i:146XS:i:78Yt:Z:MU
@@ -76,9 +76,9 @@
 A00126:224:H7HMWDSX3:3:1102:24433:32565	Test_seq	169299	Test_seq	169714	+	-	UU	A00126:224:H7HMWDSX3:3:1102:24433:3256597Test_seq16929960151M=169564416**NM:i:1MD:Z:10T140MC:Z:151MAS:i:146XS:i:106Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:24433:32565145Test_seq16956460151M=169299-416**NM:i:5MD:Z:5G58T6T4A22G51MC:Z:151MAS:i:126XS:i:66Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:24343:32910	Test_seq	169299	Test_seq	169714	+	-	UU	A00126:224:H7HMWDSX3:3:1102:24343:3291097Test_seq16929960151M=169564416**NM:i:1MD:Z:10T140MC:Z:151MAS:i:146XS:i:106Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:24343:32910145Test_seq16956460151M=169299-416**NM:i:5MD:Z:5G58T6T4A22G51MC:Z:151MAS:i:126XS:i:66Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:24379:34413	Test_seq	169290	Test_seq	169639	+	-	UU	A00126:224:H7HMWDSX3:3:1102:24379:3441397Test_seq16929060151M=169489350**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:102Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:24379:34413145Test_seq16948960151M=169290-350**NM:i:4MD:Z:30C0C41T65T11MC:Z:151MAS:i:131XS:i:70Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1102:2031:34757	!	0	!	0	-	-	nM	A00126:224:H7HMWDSX3:3:1102:2031:34757177Test_seq2135927127M24S=167554-46015**NM:i:6MD:Z:0A9G1G64T6G26C15MC:Z:151MAS:i:101XS:i:96XA:Z:Test_seq,+167316,24S127M,7;Test_seq,+400737,24S114M13S,5;Yt:Z:nM	A00126:224:H7HMWDSX3:3:1102:2031:34757113Test_seq16755431151M=21359246015**NM:i:1MD:Z:127C23MC:Z:127M24SAS:i:146XS:i:131XA:Z:Test_seq,+213334,126M2I23M,4;Yt:Z:nM
+A00126:224:H7HMWDSX3:3:1102:2031:34757	!	0	!	0	-	-	NM	A00126:224:H7HMWDSX3:3:1102:2031:34757177Test_seq2135927127M24S=167554-46015**NM:i:6MD:Z:0A9G1G64T6G26C15MC:Z:151MAS:i:101XS:i:96XA:Z:Test_seq,+167316,24S127M,7;Test_seq,+400737,24S114M13S,5;Yt:Z:NM	A00126:224:H7HMWDSX3:3:1102:2031:34757113Test_seq16755431151M=21359246015**NM:i:1MD:Z:127C23MC:Z:127M24SAS:i:146XS:i:131XA:Z:Test_seq,+213334,126M2I23M,4;Yt:Z:NM
 A00126:224:H7HMWDSX3:3:1102:12563:35650	Test_seq	346775	Test_seq	347269	+	-	UU	A00126:224:H7HMWDSX3:3:1102:12563:35650161Test_seq34677560151M=347119495**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:77Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:12563:3565081Test_seq34711960151M=346775-495**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:59Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1103:19000:1892	!	0	Test_seq	169431	-	-	MR	A00126:224:H7HMWDSX3:3:1103:19000:1892177Test_seq4000600122S29M=169281-230658**NM:i:1MD:Z:26A2MC:Z:151MAS:i:26XS:i:26SA:Z:Test_seq,168996,+,28S123M,60,2;XA:Z:Test_seq,-166639,122S29M,1;Test_seq,+214367,18M1I10M122S,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1103:19000:18922209Test_seq1689966028H123M=169281436**NM:i:2MD:Z:96T6G19MC:Z:151MAS:i:113XS:i:36SA:Z:Test_seq,400060,-,122S29M,0,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1103:19000:1892113Test_seq16928160151M=400060230658**NM:i:1MD:Z:28T122MC:Z:122S29MAS:i:146XS:i:103Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1103:19000:1892	!	0	Test_seq	169431	-	-	MR	A00126:224:H7HMWDSX3:3:1103:19000:18922209Test_seq1689966028H123M=169281436**NM:i:2MD:Z:96T6G19MC:Z:151MAS:i:113XS:i:36SA:Z:Test_seq,400060,-,122S29M,0,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1103:19000:1892177Test_seq4000600122S29M=169281-230658**NM:i:1MD:Z:26A2MC:Z:151MAS:i:26XS:i:26SA:Z:Test_seq,168996,+,28S123M,60,2;XA:Z:Test_seq,-166639,122S29M,1;Test_seq,+214367,18M1I10M122S,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1103:19000:1892113Test_seq16928160151M=400060230658**NM:i:1MD:Z:28T122MC:Z:122S29MAS:i:146XS:i:103Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1103:30544:2378	!	0	Test_seq	168612	-	-	MU	A00126:224:H7HMWDSX3:3:1103:30544:2378161Test_seq1682780151M=168462335**NM:i:12MD:Z:4T53C0A6C0G0G18A2A25T7C18C6C0MC:Z:151MAS:i:95XS:i:95XA:Z:Test_seq,-212610,151M,12;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1103:30544:237881Test_seq16846260151M=168278-335**NM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:91Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1103:25880:3098	Test_seq	183557	Test_seq	184047	+	-	UU	A00126:224:H7HMWDSX3:3:1103:25880:3098161Test_seq18355760151M=183897491**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:30Yt:Z:UU	A00126:224:H7HMWDSX3:3:1103:25880:309881Test_seq18389760151M=183557-491**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:64Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1103:26639:3818	!	0	Test_seq	214212	-	-	MU	A00126:224:H7HMWDSX3:3:1103:26639:381897Test_seq414556151M=214062172758**NM:i:13MD:Z:20A25C0G26T0C13T22C0A1T5T4C4T15C3MC:Z:151MAS:i:87XS:i:81XA:Z:Test_seq,-49064,151M,14;Test_seq,-151371,151M,15;Test_seq,+258709,151M,16;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1103:26639:3818145Test_seq21406260151M=41455-172758**NM:i:1MD:Z:148A2MC:Z:151MAS:i:148XS:i:116Yt:Z:MU
@@ -134,9 +134,9 @@
 A00126:224:H7HMWDSX3:3:1104:15076:6026	Test_seq	171233	Test_seq	171678	+	-	UU	A00126:224:H7HMWDSX3:3:1104:15076:602697Test_seq17123360151M=171528446**NM:i:1MD:Z:93A57MC:Z:151MAS:i:146XS:i:38Yt:Z:UU	A00126:224:H7HMWDSX3:3:1104:15076:6026145Test_seq17152860151M=171233-446**NM:i:5MD:Z:3T16A34T9T51C33MC:Z:151MAS:i:127XS:i:60Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1104:6325:6997	Test_seq	170855	Test_seq	171213	+	-	UU	A00126:224:H7HMWDSX3:3:1104:6325:6997161Test_seq17085560151M=171063359**NM:i:1MD:Z:104A46MC:Z:151MAS:i:146XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1104:6325:699781Test_seq17106360151M=170855-359**NM:i:5MD:Z:28A2C23C35G50C8MC:Z:151MAS:i:126XS:i:58Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1104:21658:7279	!	0	Test_seq	293960	-	-	NR	A00126:224:H7HMWDSX3:3:1104:21658:727997Test_seq2935986032S119M=293810363**NM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:21658:7279145Test_seq29381060151M=293598-363**NM:i:1MD:Z:85A65MC:Z:32S119MAS:i:146XS:i:0Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1104:8169:7498	!	0	!	0	-	-	nM	A00126:224:H7HMWDSX3:3:1104:8169:749897Test_seq167315827S124M=167558394**NM:i:2MD:Z:8A44A70MC:Z:151MAS:i:114XS:i:109XA:Z:Test_seq,-213596,124M27S,3;Test_seq,+400736,27S124M,5;Yt:Z:nM	A00126:224:H7HMWDSX3:3:1104:8169:7498145Test_seq16755831151M=167315-394**NM:i:1MD:Z:118A32MC:Z:27S124MAS:i:146XS:i:131XA:Z:Test_seq,+213330,130M2I19M,4;Yt:Z:nM
+A00126:224:H7HMWDSX3:3:1104:8169:7498	!	0	!	0	-	-	NM	A00126:224:H7HMWDSX3:3:1104:8169:749897Test_seq167315827S124M=167558394**NM:i:2MD:Z:8A44A70MC:Z:151MAS:i:114XS:i:109XA:Z:Test_seq,-213596,124M27S,3;Test_seq,+400736,27S124M,5;Yt:Z:NM	A00126:224:H7HMWDSX3:3:1104:8169:7498145Test_seq16755831151M=167315-394**NM:i:1MD:Z:118A32MC:Z:27S124MAS:i:146XS:i:131XA:Z:Test_seq,+213330,130M2I19M,4;Yt:Z:NM
 A00126:224:H7HMWDSX3:3:1104:5791:7921	!	0	Test_seq	171294	-	-	NR	A00126:224:H7HMWDSX3:3:1104:5791:7921161Test_seq1710696024S127M=171144226**NM:i:3MD:Z:5C16A2C101MC:Z:151MAS:i:112XS:i:68Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:5791:792181Test_seq17114460151M=171069-226**NM:i:1MD:Z:66C84MC:Z:24S127MAS:i:146XS:i:55Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1104:11568:9377	!	0	Test_seq	168405	-	+	MR	A00126:224:H7HMWDSX3:3:1104:11568:9377145Test_seq11604721128S23M=16840552337**NM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:19SA:Z:Test_seq,168651,-,126M25S,60,1;XA:Z:Test_seq,-85506,127S19M5S,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1104:11568:93772193Test_seq16865160126M25H=168405-372**NM:i:1MD:Z:65T60MC:Z:151MAS:i:121XS:i:55SA:Z:Test_seq,116047,-,128S23M,21,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1104:11568:937797Test_seq16840560151M=116047-52337**NM:i:1MD:Z:90T60MC:Z:128S23MAS:i:146XS:i:81Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1104:11568:9377	!	0	Test_seq	168405	-	+	MR	A00126:224:H7HMWDSX3:3:1104:11568:93772193Test_seq16865160126M25H=168405-372**NM:i:1MD:Z:65T60MC:Z:151MAS:i:121XS:i:55SA:Z:Test_seq,116047,-,128S23M,21,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1104:11568:9377145Test_seq11604721128S23M=16840552337**NM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:19SA:Z:Test_seq,168651,-,126M25S,60,1;XA:Z:Test_seq,-85506,127S19M5S,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1104:11568:937797Test_seq16840560151M=116047-52337**NM:i:1MD:Z:90T60MC:Z:128S23MAS:i:146XS:i:81Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1104:24921:11741	!	0	Test_seq	168602	-	-	MU	A00126:224:H7HMWDSX3:3:1104:24921:11741113Test_seq21265024151M=168452-44199**NM:i:11MD:Z:7G2A0G4A5T2A17C0T17T52A8G26MC:Z:151MAS:i:100XS:i:85XA:Z:Test_seq,+168238,151M,14;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1104:24921:11741177Test_seq16845260151M=21265044199**NM:i:1MD:Z:14G136MC:Z:151MAS:i:146XS:i:93Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1104:8323:13463	Test_seq	170316	Test_seq	170786	+	-	UU	A00126:224:H7HMWDSX3:3:1104:8323:13463161Test_seq17031660151M=170636471**NM:i:1MD:Z:123A27MC:Z:151MAS:i:146XS:i:52Yt:Z:UU	A00126:224:H7HMWDSX3:3:1104:8323:1346381Test_seq17063660151M=170316-471**NM:i:7MD:Z:3A42G2G3A2A38G25G29MC:Z:151MAS:i:117XS:i:23Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1104:18855:14418	!	0	Test_seq	171064	-	+	NR	A00126:224:H7HMWDSX3:3:1104:18855:1441881Test_seq17129360129M22S=171064-358**NM:i:3MD:Z:81T25G15T5MC:Z:151MAS:i:114XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:18855:14418161Test_seq17106460151M=171293358**NM:i:1MD:Z:27A123MC:Z:129M22SAS:i:146XS:i:73Yt:Z:NR
@@ -146,7 +146,7 @@
 A00126:224:H7HMWDSX3:3:1104:10926:18349	Test_seq	170851	Test_seq	171176	+	-	UU	A00126:224:H7HMWDSX3:3:1104:10926:18349161Test_seq17085160151M=171045326**NM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1104:10926:1834981Test_seq17104560132M19S=170851-326**NM:i:4MD:Z:2C43A40C0C43MC:Z:151MAS:i:114XS:i:65Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1104:7075:20478	!	0	Test_seq	171148	-	+	NR	A00126:224:H7HMWDSX3:3:1104:7075:20478145Test_seq17129360129M22S=171148-274**NM:i:3MD:Z:41G65G15T5MC:Z:151MAS:i:114XS:i:48Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:7075:2047897Test_seq17114860151M=171293274**NM:i:1MD:Z:66T84MC:Z:129M22SAS:i:146XS:i:51Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1104:7229:20525	!	0	Test_seq	171148	-	+	NR	A00126:224:H7HMWDSX3:3:1104:7229:20525145Test_seq17129360129M22S=171148-274**NM:i:3MD:Z:41G65G15T5MC:Z:151MAS:i:114XS:i:48Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:7229:2052597Test_seq17114860151M=171293274**NM:i:1MD:Z:66T84MC:Z:129M22SAS:i:146XS:i:51Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1104:27416:21355	!	0	Test_seq	167173	-	+	Mu	A00126:224:H7HMWDSX3:3:1104:27416:2135581Test_seq1673507151M=167173-328**NM:i:1MD:Z:18A132MC:Z:23S128MAS:i:146XS:i:141XA:Z:Test_seq,+213534,151M,2;Yt:Z:Mu	A00126:224:H7HMWDSX3:3:1104:27416:21355161Test_seq1671734323S128M=167350328**NM:i:2MD:Z:47T22T57MC:Z:151MAS:i:118XS:i:98XA:Z:Test_seq,-213734,128M23S,6;Yt:Z:Mu
+A00126:224:H7HMWDSX3:3:1104:27416:21355	!	0	Test_seq	167173	-	+	MU	A00126:224:H7HMWDSX3:3:1104:27416:2135581Test_seq1673507151M=167173-328**NM:i:1MD:Z:18A132MC:Z:23S128MAS:i:146XS:i:141XA:Z:Test_seq,+213534,151M,2;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1104:27416:21355161Test_seq1671734323S128M=167350328**NM:i:2MD:Z:47T22T57MC:Z:151MAS:i:118XS:i:98XA:Z:Test_seq,-213734,128M23S,6;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1104:28411:22075	!	0	Test_seq	203500	-	+	MR	A00126:224:H7HMWDSX3:3:1104:28411:2207565Test_seq2147512737M114S=203500-11252**NM:i:0MD:Z:37MC:Z:151MAS:i:37XS:i:32SA:Z:Test_seq,203705,-,115M36S,60,0;XA:Z:Test_seq,-399667,114S37M,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1104:28411:220752129Test_seq20370560115M36H=203500-320**NM:i:0MD:Z:115MC:Z:151MAS:i:115XS:i:0SA:Z:Test_seq,214751,+,37M114S,27,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1104:28411:22075129Test_seq20350060151M=21475111252**NM:i:0MD:Z:151MC:Z:37M114SAS:i:151XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1104:19804:22701	!	0	Test_seq	168041	-	-	MU	A00126:224:H7HMWDSX3:3:1104:19804:22701161Test_seq1679741268M=16797468**NM:i:6MD:Z:8G2A0T4A6G16C26MC:Z:68MAS:i:40XS:i:33XA:Z:Test_seq,-212997,43M25S,2;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1104:19804:2270181Test_seq1679746068M=167974-68**NM:i:1MD:Z:20G47MC:Z:68MAS:i:63XS:i:42Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1104:28718:23547	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1104:28718:2354797Test_seq3334991179M=15135-318287**NM:i:1MD:Z:76G2MC:Z:79MAS:i:76XS:i:71XA:Z:Test_seq,-383985,79M,2;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1104:28718:23547145Test_seq15135679M=333499318287**NM:i:4MD:Z:9G40C2C22G2MC:Z:79MAS:i:61XS:i:56XA:Z:Test_seq,+383985,79M,5;Test_seq,-184559,79M,5;Test_seq,-395912,79M,5;Test_seq,-47050,79M,6;Test_seq,-333499,79M,6;Yt:Z:MM
@@ -179,10 +179,10 @@
 A00126:224:H7HMWDSX3:3:1105:27724:12399	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1105:27724:1239997Test_seq384489552M=38449952**NM:i:1MD:Z:1C50MC:Z:10S42MAS:i:50XS:i:47XA:Z:Test_seq,-333022,52M,1;Test_seq,+204890,3S49M,1;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1105:27724:12399145Test_seq384499410S42M=384489-52**NM:i:0MD:Z:42MC:Z:52MAS:i:42XS:i:40Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1105:30861:14951	!	0	Test_seq	171173	-	+	NR	A00126:224:H7HMWDSX3:3:1105:30861:14951145Test_seq17150260127M24S=171173-456**NM:i:7MD:Z:23A4A39G5G6T9T7G27MC:Z:151MAS:i:92XS:i:25Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:30861:1495197Test_seq17117360151M=171502456**NM:i:1MD:Z:8C142MC:Z:127M24SAS:i:146XS:i:36Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:7419:15436	!	0	Test_seq	214052	-	+	MU	A00126:224:H7HMWDSX3:3:1105:7419:15436145Test_seq21426836117M1I33M=214052-366**NM:i:6MD:Z:2A22T45T29C13T34MC:Z:151MAS:i:120XS:i:100XA:Z:Test_seq,+400037,151M,11;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1105:7419:1543697Test_seq21405260151M=214268366**NM:i:1MD:Z:109T41MC:Z:117M1I33MAS:i:146XS:i:106Yt:Z:MU
-A00126:224:H7HMWDSX3:3:1105:23077:15562	!	0	!	0	-	-	nM	A00126:224:H7HMWDSX3:3:1105:23077:15562161Test_seq1671312225S126M=167359379**NM:i:2MD:Z:13C98T13MC:Z:151MAS:i:116XS:i:106XA:Z:Test_seq,-213778,126M25S,4;Yt:Z:nM	A00126:224:H7HMWDSX3:3:1105:23077:1556281Test_seq16735917151M=167131-379**NM:i:1MD:Z:9A141MC:Z:25S126MAS:i:146XS:i:138XA:Z:Test_seq,+213528,3S148M,2;Yt:Z:nM
+A00126:224:H7HMWDSX3:3:1105:23077:15562	!	0	!	0	-	-	NM	A00126:224:H7HMWDSX3:3:1105:23077:15562161Test_seq1671312225S126M=167359379**NM:i:2MD:Z:13C98T13MC:Z:151MAS:i:116XS:i:106XA:Z:Test_seq,-213778,126M25S,4;Yt:Z:NM	A00126:224:H7HMWDSX3:3:1105:23077:1556281Test_seq16735917151M=167131-379**NM:i:1MD:Z:9A141MC:Z:25S126MAS:i:146XS:i:138XA:Z:Test_seq,+213528,3S148M,2;Yt:Z:NM
 A00126:224:H7HMWDSX3:3:1105:27923:18443	!	0	Test_seq	168451	-	+	NR	A00126:224:H7HMWDSX3:3:1105:27923:1844381Test_seq16868960122M29S=168451-360**NM:i:1MD:Z:27T94MC:Z:151MAS:i:117XS:i:54Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:27923:18443161Test_seq16845160151M=168689360**NM:i:1MD:Z:44T106MC:Z:122M29SAS:i:146XS:i:93Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:8820:20337	Test_seq	171233	Test_seq	171572	+	-	UU	A00126:224:H7HMWDSX3:3:1105:8820:20337161Test_seq17123360151M=171441340**NM:i:1MD:Z:31G119MC:Z:132M19SAS:i:146XS:i:36Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:8820:2033781Test_seq17144160132M19S=171233-340**NM:i:3MD:Z:73A10A7C39MC:Z:151MAS:i:117XS:i:43Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1105:10276:21010	!	0	Test_seq	171288	-	-	MR	A00126:224:H7HMWDSX3:3:1105:10276:21010113Test_seq518770132S19M=171138119394**NM:i:0MD:Z:19MC:Z:151MAS:i:19XS:i:0SA:Z:Test_seq,171070,+,19S132M,60,3;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1105:10276:210102145Test_seq1710706019H132M=171138219**NM:i:3MD:Z:16T4A23G86MC:Z:151MAS:i:117XS:i:74SA:Z:Test_seq,51877,-,132S19M,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1105:10276:21010177Test_seq17113860151M=51877-119394**NM:i:1MD:Z:105T45MC:Z:132S19MAS:i:146XS:i:0Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1105:10276:21010	!	0	Test_seq	171288	-	-	MR	A00126:224:H7HMWDSX3:3:1105:10276:210102145Test_seq1710706019H132M=171138219**NM:i:3MD:Z:16T4A23G86MC:Z:151MAS:i:117XS:i:74SA:Z:Test_seq,51877,-,132S19M,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1105:10276:21010113Test_seq518770132S19M=171138119394**NM:i:0MD:Z:19MC:Z:151MAS:i:19XS:i:0SA:Z:Test_seq,171070,+,19S132M,60,3;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1105:10276:21010177Test_seq17113860151M=51877-119394**NM:i:1MD:Z:105T45MC:Z:132S19MAS:i:146XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1105:18837:21089	Test_seq	165754	Test_seq	166211	+	-	UU	A00126:224:H7HMWDSX3:3:1105:18837:2108997Test_seq16575460151M=166061458**NM:i:3MD:Z:40A5T75G28MC:Z:151MAS:i:136XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:18837:21089145Test_seq16606160151M=165754-458**NM:i:1MD:Z:82C68MC:Z:151MAS:i:146XS:i:75Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1105:16640:21167	!	0	Test_seq	167661	-	-	NR	A00126:224:H7HMWDSX3:3:1105:16640:2116797Test_seq167316722S129M=167511346**NM:i:4MD:Z:7A37G6A73T2MC:Z:151MAS:i:111XS:i:106XA:Z:Test_seq,-213590,129M22S,5;Test_seq,+400737,22S123M6S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:16640:21167145Test_seq16751160151M=167316-346**NM:i:0MD:Z:151MC:Z:22S129MAS:i:151XS:i:122XA:Z:Test_seq,+213377,83M2I23M2I37M4S,5;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:7835:24173	Test_seq	171202	Test_seq	171530	+	-	UU	A00126:224:H7HMWDSX3:3:1105:7835:2417397Test_seq17120260151M=171381329**NM:i:1MD:Z:108A42MC:Z:101M1I49MAS:i:146XS:i:76Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:7835:24173145Test_seq17138160101M1I49M=171202-329**NM:i:8MD:Z:19G3C2C8T15G46T45A5MC:Z:151MAS:i:108XS:i:28Yt:Z:UU
@@ -215,7 +215,7 @@
 A00126:224:H7HMWDSX3:3:1106:18222:16736	!	0	Test_seq	168630	-	-	MU	A00126:224:H7HMWDSX3:3:1106:18222:1673697Test_seq16831411151M=168480317**NM:i:15MD:Z:22C0A6C0G0G18A5G9C12T7C20A13G6A1G0T17MC:Z:151MAS:i:76XS:i:68XA:Z:Test_seq,-212574,151M,17;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1106:18222:16736145Test_seq16848060151M=168314-317**NM:i:1MD:Z:15T135MC:Z:151MAS:i:146XS:i:91Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1106:18747:17018	Test_seq	170380	Test_seq	170837	+	-	UU	A00126:224:H7HMWDSX3:3:1106:18747:17018161Test_seq17038060151M=170691458**NM:i:1MD:Z:44C106MC:Z:73M4I74MAS:i:146XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:18747:1701881Test_seq1706916073M4I74M=170380-458**NM:i:5MD:Z:40G106MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:16251:18740	Test_seq	225248	Test_seq	308334	+	+	UR	A00126:224:H7HMWDSX3:3:1106:16251:18740129Test_seq2252486029M122S=30833483087**NM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,308589,-,122M29S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1106:16251:187402193Test_seq30858960122M29H=308334-377**NM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:24SA:Z:Test_seq,225248,+,29M122S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1106:16251:1874065Test_seq30833460151M=225248-83087**NM:i:0MD:Z:151MC:Z:29M122SAS:i:151XS:i:35Yt:Z:UR
-A00126:224:H7HMWDSX3:3:1106:31901:18756	!	0	Test_seq	56593	-	+	MR	A00126:224:H7HMWDSX3:3:1106:31901:1875665Test_seq115194245S23M123S=56593-58602**NM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,56816,-,123M28S,60,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:31901:187562129Test_seq5681660123M28H=56593-346**NM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0SA:Z:Test_seq,115194,+,5S23M123S,24,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1106:31901:18756129Test_seq5659360151M=11519458602**NM:i:0MD:Z:151MC:Z:5S23M123SAS:i:151XS:i:103Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1106:31901:18756	!	0	Test_seq	56593	-	+	MR	A00126:224:H7HMWDSX3:3:1106:31901:187562129Test_seq5681660123M28H=56593-346**NM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0SA:Z:Test_seq,115194,+,5S23M123S,24,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:31901:1875665Test_seq115194245S23M123S=56593-58602**NM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,56816,-,123M28S,60,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1106:31901:18756129Test_seq5659360151M=11519458602**NM:i:0MD:Z:151MC:Z:5S23M123SAS:i:151XS:i:103Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1106:24795:22357	Test_seq	169327	Test_seq	169680	+	-	UU	A00126:224:H7HMWDSX3:3:1106:24795:22357161Test_seq16932760151M=169530354**NM:i:1MD:Z:140G10MC:Z:151MAS:i:146XS:i:111Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:24795:2235781Test_seq16953057151M=169327-354**NM:i:8MD:Z:27C11G58T5T0T0T3A22G17MC:Z:151MAS:i:111XS:i:78Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:9127:23187	Test_seq	168739	Test_seq	169233	+	-	UU	A00126:224:H7HMWDSX3:3:1106:9127:23187161Test_seq16873960151M=169083495**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:81Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:9127:2318781Test_seq16908360151M=168739-495**NM:i:7MD:Z:8C62G2T3G7C31C26A5MC:Z:151MAS:i:116XS:i:65Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:17309:23328	Test_seq	168424	Test_seq	168810	+	-	UU	A00126:224:H7HMWDSX3:3:1106:17309:23328161Test_seq16842460151M=168660387**NM:i:3MD:Z:13G57T50T28MC:Z:151MAS:i:136XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:17309:2332881Test_seq16866060151M=168424-387**NM:i:1MD:Z:56T94MC:Z:151MAS:i:146XS:i:55Yt:Z:UU
@@ -223,7 +223,7 @@
 A00126:224:H7HMWDSX3:3:1106:27308:24862	!	0	Test_seq	167312	-	-	MU	A00126:224:H7HMWDSX3:3:1106:27308:24862113Test_seq21395037151M=167162-46789**NM:i:4MD:Z:28C14A49T11A45MC:Z:151MAS:i:131XS:i:112XA:Z:Test_seq,+166938,4S147M,7;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1106:27308:24862177Test_seq16716252151M=21395046789**NM:i:1MD:Z:81T69MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213722,151M,6;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1106:2284:29434	!	0	Test_seq	167543	-	-	NR	A00126:224:H7HMWDSX3:3:1106:2284:2943497Test_seq1671734428S123M=167393371**NM:i:2MD:Z:5A64T52MC:Z:151MAS:i:113XS:i:93XA:Z:Test_seq,-213739,123M28S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1106:2284:29434145Test_seq16739360151M=167173-371**NM:i:1MD:Z:118T32MC:Z:28S123MAS:i:146XS:i:117XA:Z:Test_seq,+213491,29M4D4M4I114M,10;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1106:3125:29763	Test_seq	169989	Test_seq	170460	+	-	UU	A00126:224:H7HMWDSX3:3:1106:3125:29763161Test_seq16998948151M=170310472**NM:i:10MD:Z:33G5C10A7A1G5C2G48C7G20A3MC:Z:151MAS:i:102XS:i:73Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:3125:2976381Test_seq17031060151M=169989-472**NM:i:1MD:Z:66C84MC:Z:151MAS:i:146XS:i:63Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1106:29026:29841	!	0	Test_seq	166159	-	+	MR	A00126:224:H7HMWDSX3:3:1106:29026:2984165Test_seq38347205S21M125S=166159-217314**NM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:21SA:Z:Test_seq,166429,-,128M23S,60,2;XA:Z:Test_seq,-334091,125S26M,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:29026:298412129Test_seq16642960128M23H=166159-398**NM:i:2MD:Z:95C18A13MC:Z:151MAS:i:118XS:i:28SA:Z:Test_seq,383472,+,5S21M125S,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1106:29026:29841129Test_seq16615960151M=383472217314**NM:i:1MD:Z:102T48MC:Z:5S21M125SAS:i:146XS:i:77Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1106:29026:29841	!	0	Test_seq	166159	-	+	MR	A00126:224:H7HMWDSX3:3:1106:29026:298412129Test_seq16642960128M23H=166159-398**NM:i:2MD:Z:95C18A13MC:Z:151MAS:i:118XS:i:28SA:Z:Test_seq,383472,+,5S21M125S,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:29026:2984165Test_seq38347205S21M125S=166159-217314**NM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:21SA:Z:Test_seq,166429,-,128M23S,60,2;XA:Z:Test_seq,-334091,125S26M,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1106:29026:29841129Test_seq16615960151M=383472217314**NM:i:1MD:Z:102T48MC:Z:5S21M125SAS:i:146XS:i:77Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1106:15591:30123	Test_seq	171134	Test_seq	171455	+	-	UU	A00126:224:H7HMWDSX3:3:1106:15591:3012397Test_seq17113460151M=171323322**NM:i:1MD:Z:118T32MC:Z:133M18SAS:i:146XS:i:63Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:15591:30123145Test_seq17132360133M18S=171134-322**NM:i:3MD:Z:46G30G15T39MC:Z:151MAS:i:118XS:i:38Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:30138:30483	Test_seq	183328	Test_seq	183714	+	-	UU	A00126:224:H7HMWDSX3:3:1106:30138:30483161Test_seq1833286026M4I121M=183564387**NM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:30138:3048381Test_seq18356460151M=183328-387**NM:i:0MD:Z:151MC:Z:26M4I121MAS:i:151XS:i:52Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:15890:30639	Test_seq	171134	Test_seq	171455	+	-	UU	A00126:224:H7HMWDSX3:3:1106:15890:3063997Test_seq17113460151M=171323322**NM:i:1MD:Z:118T32MC:Z:133M18SAS:i:146XS:i:63Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:15890:30639145Test_seq17132360133M18S=171134-322**NM:i:3MD:Z:46G30G15T39MC:Z:151MAS:i:118XS:i:38Yt:Z:UU
@@ -232,7 +232,7 @@
 A00126:224:H7HMWDSX3:3:1106:14362:33599	Test_seq	167148	Test_seq	167571	+	-	UU	A00126:224:H7HMWDSX3:3:1106:14362:3359997Test_seq16714841151M=167421424**NM:i:2MD:Z:30A64T55MC:Z:151MAS:i:141XS:i:121XA:Z:Test_seq,-213736,151M,6;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:14362:33599145Test_seq16742160151M=167148-424**NM:i:1MD:Z:85A65MC:Z:151MAS:i:146XS:i:106Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:27236:35697	Test_seq	406947	Test_seq	407376	+	-	UU	A00126:224:H7HMWDSX3:3:1106:27236:35697161Test_seq4069476061M4I86M=407226430**NM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:27236:3569781Test_seq40722660151M=406947-430**NM:i:0MD:Z:151MC:Z:61M4I86MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:29008:36886	!	0	Test_seq	168330	-	-	MU	A00126:224:H7HMWDSX3:3:1106:29008:36886161Test_seq16797237151M=168180359**NM:i:10MD:Z:1T12T13T50G8C35C4T3A11G1G3MC:Z:151MAS:i:108XS:i:86Yt:Z:MU	A00126:224:H7HMWDSX3:3:1106:29008:3688681Test_seq16818042151M=167972-359**NM:i:1MD:Z:91T59MC:Z:151MAS:i:146XS:i:126XA:Z:Test_seq,+212708,151M,5;Yt:Z:MU
-A00126:224:H7HMWDSX3:3:1107:20654:1313	!	0	Test_seq	435035	-	-	MR	A00126:224:H7HMWDSX3:3:1107:20654:1313177Test_seq38260035125S26M=43488552411**NM:i:1MD:Z:25A0MC:Z:151MAS:i:25XS:i:0SA:Z:Test_seq,434625,+,26S125M,60,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1107:20654:13132209Test_seq4346256026H125M=434885411**NM:i:0MD:Z:125MC:Z:151MAS:i:125XS:i:0SA:Z:Test_seq,382600,-,125S26M,35,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1107:20654:1313113Test_seq43488560151M=382600-52411**NM:i:0MD:Z:151MC:Z:125S26MAS:i:151XS:i:0Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1107:20654:1313	!	0	Test_seq	435035	-	-	MR	A00126:224:H7HMWDSX3:3:1107:20654:13132209Test_seq4346256026H125M=434885411**NM:i:0MD:Z:125MC:Z:151MAS:i:125XS:i:0SA:Z:Test_seq,382600,-,125S26M,35,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1107:20654:1313177Test_seq38260035125S26M=43488552411**NM:i:1MD:Z:25A0MC:Z:151MAS:i:25XS:i:0SA:Z:Test_seq,434625,+,26S125M,60,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1107:20654:1313113Test_seq43488560151M=382600-52411**NM:i:0MD:Z:151MC:Z:125S26MAS:i:151XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1107:4788:1892	Test_seq	166087	Test_seq	166508	+	-	UU	A00126:224:H7HMWDSX3:3:1107:4788:189297Test_seq16608760151M=166358422**NM:i:1MD:Z:16C134MC:Z:151MAS:i:146XS:i:74Yt:Z:UU	A00126:224:H7HMWDSX3:3:1107:4788:1892145Test_seq16635860151M=166087-422**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:43Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1107:7120:2801	Test_seq	165764	Test_seq	166210	+	-	UU	A00126:224:H7HMWDSX3:3:1107:7120:280197Test_seq1657646016S135M=166060447**NM:i:4MD:Z:10C25T66A8G22MC:Z:151MAS:i:115XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1107:7120:2801145Test_seq16606060151M=165764-447**NM:i:1MD:Z:107T43MC:Z:16S135MAS:i:146XS:i:75Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1107:29279:4225	!	0	Test_seq	167906	-	+	MU	A00126:224:H7HMWDSX3:3:1107:29279:4225145Test_seq16819839151M=167906-443**NM:i:4MD:Z:16G56T18C55G2MC:Z:151MAS:i:133XS:i:113XA:Z:Test_seq,+212690,151M,8;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1107:29279:422597Test_seq16790660151M=168198443**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:41Yt:Z:MU
@@ -274,11 +274,11 @@
 A00126:224:H7HMWDSX3:3:1108:9634:5588	!	0	Test_seq	212883	-	-	MU	A00126:224:H7HMWDSX3:3:1108:9634:5588161Test_seq40166179M3I69M=212733172718**NM:i:16MD:Z:15T9A5C11T21G9C5T0G3T9T6G28A0T14MC:Z:151MAS:i:74XS:i:71XA:Z:Test_seq,+212488,151M,16;Test_seq,-168400,151M,17;Test_seq,-50359,64M13I74M,24;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1108:9634:558881Test_seq21273360151M=40166-172718**NM:i:1MD:Z:104A46MC:Z:79M3I69MAS:i:146XS:i:116Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1108:16071:6872	!	0	Test_seq	167548	-	+	MR	A00126:224:H7HMWDSX3:3:1108:16071:6872129Test_seq361031026M125S=167548131446**NM:i:1MD:Z:20T5MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,167824,-,126M25S,60,4;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1108:16071:68722193Test_seq16782460126M25H=167548-402**NM:i:4MD:Z:0T60C24C32C6MC:Z:151MAS:i:110XS:i:75SA:Z:Test_seq,36103,+,26M125S,10,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1108:16071:687265Test_seq16754842151M=36103-131446**NM:i:1MD:Z:127C23MC:Z:26M125SAS:i:146XS:i:126XA:Z:Test_seq,-213340,120M2I24M5S,4;Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1108:7292:7576	!	0	Test_seq	167624	-	-	MU	A00126:224:H7HMWDSX3:3:1108:7292:7576161Test_seq1672982119S132M=167474327**NM:i:2MD:Z:25A44A61MC:Z:151MAS:i:122XS:i:112XA:Z:Test_seq,-213605,132M19S,4;Test_seq,+400719,19S132M,6;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1108:7292:757681Test_seq16747460151M=167298-327**NM:i:0MD:Z:151MC:Z:19S132MAS:i:151XS:i:104Yt:Z:MU
-A00126:224:H7HMWDSX3:3:1108:24126:7701	Test_seq	55744	Test_seq	65171	+	-	RU	A00126:224:H7HMWDSX3:3:1108:24126:770197Test_seq5574460151M=651449428**NM:i:0MD:Z:151MC:Z:123S28MAS:i:151XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:24126:7701145Test_seq6514454123S28M=55744-9428**NM:i:0MD:Z:28MC:Z:151MAS:i:28XS:i:0SA:Z:Test_seq,56015,-,123M28S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:24126:77012193Test_seq5601560123M28H=55744-394**NM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:23SA:Z:Test_seq,65144,-,123S28M,54,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1108:24126:7701	Test_seq	55744	Test_seq	65171	+	-	RU	A00126:224:H7HMWDSX3:3:1108:24126:770197Test_seq5574460151M=651449428**NM:i:0MD:Z:151MC:Z:123S28MAS:i:151XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:24126:77012193Test_seq5601560123M28H=55744-394**NM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:23SA:Z:Test_seq,65144,-,123S28M,54,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:24126:7701145Test_seq6514454123S28M=55744-9428**NM:i:0MD:Z:28MC:Z:151MAS:i:28XS:i:0SA:Z:Test_seq,56015,-,123M28S,60,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1108:21061:8187	Test_seq	168347	Test_seq	168776	+	-	UU	A00126:224:H7HMWDSX3:3:1108:21061:8187161Test_seq1683476017S134M=168626430**NM:i:2MD:Z:18A21G93MC:Z:151MAS:i:124XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:21061:818781Test_seq16862660151M=168347-430**NM:i:1MD:Z:90T60MC:Z:17S134MAS:i:146XS:i:72Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:18909:8469	Test_seq	104742	Test_seq	114432	+	-	UR	A00126:224:H7HMWDSX3:3:1108:18909:846997Test_seq1047426031M120S=1142829691**NM:i:0MD:Z:31MC:Z:151MAS:i:31XS:i:0SA:Z:Test_seq,114039,+,30S121M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1108:18909:84692145Test_seq1140396030H121M=114282394**NM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:73SA:Z:Test_seq,104742,+,31M120S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1108:18909:8469145Test_seq11428260151M=104742-9691**NM:i:1MD:Z:81T69MC:Z:31M120SAS:i:146XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1108:14498:8656	Test_seq	171559	Test_seq	171939	+	-	UU	A00126:224:H7HMWDSX3:3:1108:14498:865697Test_seq17155960151M=171789381**NM:i:8MD:Z:15G8T17G5T41C10T30C13C4MC:Z:151MAS:i:111XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:14498:8656145Test_seq17178960151M=171559-381**NM:i:1MD:Z:149C1MC:Z:151MAS:i:149XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1108:10031:9471	!	0	Test_seq	167173	-	+	Mu	A00126:224:H7HMWDSX3:3:1108:10031:9471145Test_seq16734221151M=167173-320**NM:i:1MD:Z:26A124MC:Z:30S121MAS:i:146XS:i:136XA:Z:Test_seq,+213542,151M,3;Yt:Z:Mu	A00126:224:H7HMWDSX3:3:1108:10031:947197Test_seq1671734130S121M=167342320**NM:i:4MD:Z:5A64T25T6T17MC:Z:151MAS:i:101XS:i:81XA:Z:Test_seq,-213741,121M30S,8;Yt:Z:Mu
+A00126:224:H7HMWDSX3:3:1108:10031:9471	!	0	Test_seq	167173	-	+	MU	A00126:224:H7HMWDSX3:3:1108:10031:9471145Test_seq16734221151M=167173-320**NM:i:1MD:Z:26A124MC:Z:30S121MAS:i:146XS:i:136XA:Z:Test_seq,+213542,151M,3;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1108:10031:947197Test_seq1671734130S121M=167342320**NM:i:4MD:Z:5A64T25T6T17MC:Z:151MAS:i:101XS:i:81XA:Z:Test_seq,-213741,121M30S,8;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1108:15148:12007	Test_seq	169307	Test_seq	169699	+	-	UU	A00126:224:H7HMWDSX3:3:1108:15148:12007161Test_seq16930760151M=169549393**NM:i:1MD:Z:147T3MC:Z:151MAS:i:147XS:i:103Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:15148:1200781Test_seq16954960151M=169307-393**NM:i:3MD:Z:9G69T34G36MC:Z:151MAS:i:136XS:i:28Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:8576:12962	Test_seq	169329	Test_seq	169811	+	-	UU	A00126:224:H7HMWDSX3:3:1108:8576:1296297Test_seq16932960151M=169665483**NM:i:0MD:Z:151MC:Z:4S147MAS:i:151XS:i:111Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:8576:12962145Test_seq169665604S147M=169329-483**NM:i:4MD:Z:36G45C10C52T0MC:Z:151MAS:i:131XS:i:76Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:19253:12884	Test_seq	146453	Test_seq	146833	+	-	UU	A00126:224:H7HMWDSX3:3:1108:19253:1288497Test_seq14645360151M=146683381**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:19253:12884145Test_seq14668360151M=146453-381**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
@@ -291,7 +291,7 @@
 A00126:224:H7HMWDSX3:3:1108:30544:19789	!	0	Test_seq	167661	-	-	NR	A00126:224:H7HMWDSX3:3:1108:30544:1978997Test_seq167316722S129M=167511346**NM:i:5MD:Z:7A13G23G6A43G32MC:Z:151MAS:i:104XS:i:99XA:Z:Test_seq,-213590,129M22S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:30544:19789145Test_seq16751160151M=167316-346**NM:i:1MD:Z:48T102MC:Z:22S129MAS:i:146XS:i:117XA:Z:Test_seq,+213377,83M2I23M2I37M4S,6;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1108:8187:20400	Test_seq	77443	Test_seq	233514	+	+	RU	A00126:224:H7HMWDSX3:3:1108:8187:2040065Test_seq7744360151M=233514156072**NM:i:1MD:Z:42G108MC:Z:27M124SAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:8187:20400129Test_seq2335144827M124S=77443-156072**NM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,77734,-,124M27S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:8187:204002193Test_seq7773460124M27H=77443-415**NM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:0SA:Z:Test_seq,233514,+,27M124S,48,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1108:8495:20494	!	0	Test_seq	162287	-	+	NR	A00126:224:H7HMWDSX3:3:1108:8495:20494145Test_seq16242260126M25S=162287-261**NM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:8495:2049497Test_seq16228760151M=162422261**NM:i:1MD:Z:66C84MC:Z:126M25SAS:i:146XS:i:0Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1108:7744:20509	!	0	Test_seq	169333	-	+	MR	A00126:224:H7HMWDSX3:3:1108:7744:20509145Test_seq17014430127S24M=169333-835**NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,169626,-,121M30S,60,4;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1108:7744:205092193Test_seq16962660121M30H=169333-414**NM:i:4MD:Z:2T11A22G70A12MC:Z:151MAS:i:103XS:i:0SA:Z:Test_seq,170144,-,127S24M,30,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1108:7744:2050997Test_seq16933360151M=170144835**NM:i:1MD:Z:134G16MC:Z:127S24MAS:i:146XS:i:109Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1108:7744:20509	!	0	Test_seq	169333	-	+	MR	A00126:224:H7HMWDSX3:3:1108:7744:205092193Test_seq16962660121M30H=169333-414**NM:i:4MD:Z:2T11A22G70A12MC:Z:151MAS:i:103XS:i:0SA:Z:Test_seq,170144,-,127S24M,30,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1108:7744:20509145Test_seq17014430127S24M=169333-835**NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,169626,-,121M30S,60,4;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1108:7744:2050997Test_seq16933360151M=170144835**NM:i:1MD:Z:134G16MC:Z:127S24MAS:i:146XS:i:109Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1108:16233:21339	Test_seq	197163	Test_seq	197474	+	-	UU	A00126:224:H7HMWDSX3:3:1108:16233:21339161Test_seq19716360151M=197343312**NM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:16233:2133981Test_seq19734360132M19S=197163-312**NM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:16342:23437	!	0	Test_seq	171131	-	+	NR	A00126:224:H7HMWDSX3:3:1108:16342:23437145Test_seq17129460128M23S=171131-291**NM:i:2MD:Z:106G15T5MC:Z:151MAS:i:118XS:i:43Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:16342:2343797Test_seq17113160151M=171294291**NM:i:1MD:Z:71C79MC:Z:128M23SAS:i:146XS:i:75Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1108:16740:23625	Test_seq	170381	Test_seq	170830	+	-	UU	A00126:224:H7HMWDSX3:3:1108:16740:23625161Test_seq17038160151M=170680450**NM:i:1MD:Z:140A10MC:Z:151MAS:i:146XS:i:47Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:16740:2362581Test_seq17068060151M=170381-450**NM:i:4MD:Z:0T4G29C15G99MC:Z:151MAS:i:135XS:i:0Yt:Z:UU
@@ -339,7 +339,7 @@
 A00126:224:H7HMWDSX3:3:1109:31367:22592	!	0	Test_seq	167451	-	+	MU	A00126:224:H7HMWDSX3:3:1109:31367:2259265Test_seq2131687151M=167451-45718**NM:i:1MD:Z:144A6MC:Z:81M4I66MAS:i:146XS:i:141XA:Z:Test_seq,-167720,151M,2;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1109:31367:22592129Test_seq1674515481M4I66M=21316845718**NM:i:8MD:Z:25A36A0T56C26MC:Z:151MAS:i:117XS:i:87Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1109:14751:22748	Test_seq	171454	Test_seq	171939	+	-	UU	A00126:224:H7HMWDSX3:3:1109:14751:2274897Test_seq1714546028M1I122M=171789486**NM:i:6MD:Z:71A2T19A34T9T10MC:Z:151MAS:i:118XS:i:32Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:14751:22748145Test_seq17178960151M=171454-486**NM:i:1MD:Z:149C1MC:Z:28M1I122MAS:i:149XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:32579:24314	Test_seq	132878	Test_seq	133237	+	-	UU	A00126:224:H7HMWDSX3:3:1109:32579:2431497Test_seq13287860151M=133104360**NM:i:0MD:Z:151MC:Z:134M17SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:32579:24314145Test_seq13310460134M17S=132878-360**NM:i:1MD:Z:19T114MC:Z:151MAS:i:129XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1109:17246:27352	!	0	Test_seq	81985	-	+	MR	A00126:224:H7HMWDSX3:3:1109:17246:27352145Test_seq9744012130S21M=81985-15476**NM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,82184,-,132M19S,60,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1109:17246:273522193Test_seq8218460132M19H=81985-331**NM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:21SA:Z:Test_seq,97440,-,130S21M,12,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1109:17246:2735297Test_seq8198560151M=9744015476**NM:i:0MD:Z:151MC:Z:130S21MAS:i:151XS:i:81Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1109:17246:27352	!	0	Test_seq	81985	-	+	MR	A00126:224:H7HMWDSX3:3:1109:17246:273522193Test_seq8218460132M19H=81985-331**NM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:21SA:Z:Test_seq,97440,-,130S21M,12,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1109:17246:27352145Test_seq9744012130S21M=81985-15476**NM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,82184,-,132M19S,60,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1109:17246:2735297Test_seq8198560151M=9744015476**NM:i:0MD:Z:151MC:Z:130S21MAS:i:151XS:i:81Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1109:17788:27821	Test_seq	168737	Test_seq	169220	+	-	UU	A00126:224:H7HMWDSX3:3:1109:17788:27821161Test_seq16873760151M=169070484**NM:i:1MD:Z:38T112MC:Z:151MAS:i:146XS:i:81Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:17788:2782181Test_seq16907060151M=168737-484**NM:i:7MD:Z:84G2T3G7C31C6A6G5MC:Z:151MAS:i:116XS:i:66Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:12346:31078	Test_seq	171229	Test_seq	171627	+	-	UU	A00126:224:H7HMWDSX3:3:1109:12346:31078161Test_seq17122960151M=171476399**NM:i:1MD:Z:114G36MC:Z:19M1D132MAS:i:146XS:i:27Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:12346:3107881Test_seq1714766019M1D132M=171229-399**NM:i:4MD:Z:13T5^T29A74C27MC:Z:151MAS:i:129XS:i:26Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:30852:31782	Test_seq	166084	Test_seq	166553	+	-	UU	A00126:224:H7HMWDSX3:3:1109:30852:3178297Test_seq16608460151M=166403470**NM:i:4MD:Z:8T77A26G26G10MC:Z:151MAS:i:131XS:i:71Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:30852:31782145Test_seq16640360151M=166084-470**NM:i:1MD:Z:102T48MC:Z:151MAS:i:146XS:i:40Yt:Z:UU
@@ -353,7 +353,7 @@
 A00126:224:H7HMWDSX3:3:1110:12020:6057	!	0	Test_seq	171156	-	+	NR	A00126:224:H7HMWDSX3:3:1110:12020:605781Test_seq17135060121M30S=171156-315**NM:i:2MD:Z:50G15T54MC:Z:151MAS:i:111XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1110:12020:6057161Test_seq17115660151M=171350315**NM:i:0MD:Z:151MC:Z:121M30SAS:i:151XS:i:35Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1110:15130:6151	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1110:15130:615165Test_seq333515062M=38398650472**NM:i:1MD:Z:51T10MC:Z:62MAS:i:57XS:i:57XA:Z:Test_seq,-383986,62M,1;Test_seq,+15151,62M,2;Test_seq,+316240,19M2I41M,3;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1110:15130:6151129Test_seq383986062M=333515-50472**NM:i:1MD:Z:10A51MC:Z:62MAS:i:57XS:i:57XA:Z:Test_seq,-333515,62M,1;Test_seq,-15151,62M,2;Test_seq,-316240,19M2I41M,3;Test_seq,+449331,62M,4;Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1110:18936:8046	Test_seq	168392	Test_seq	168721	+	-	UU	A00126:224:H7HMWDSX3:3:1110:18936:8046161Test_seq16839260151M=168571330**NM:i:6MD:Z:2T52T1C24A20T44A2MC:Z:151MAS:i:125XS:i:73Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:18936:804681Test_seq16857160151M=168392-330**NM:i:1MD:Z:145T5MC:Z:151MAS:i:146XS:i:73Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1110:19090:8406	!	0	Test_seq	169404	-	-	MR	A00126:224:H7HMWDSX3:3:1110:19090:8406177Test_seq523240127S24M=169254117058**NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:24SA:Z:Test_seq,168994,+,22S129M,60,1;XA:Z:Test_seq,-154647,127S24M,0;Test_seq,+255573,24M127S,0;Test_seq,+38332,24M127S,0;Test_seq,-170380,127S24M,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1110:19090:84062209Test_seq1689946022H129M=169254411**NM:i:1MD:Z:44G84MC:Z:151MAS:i:124XS:i:49SA:Z:Test_seq,52324,-,127S24M,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1110:19090:8406113Test_seq16925460151M=52324-117058**NM:i:1MD:Z:14T136MC:Z:127S24MAS:i:146XS:i:82Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1110:19090:8406	!	0	Test_seq	169404	-	-	MR	A00126:224:H7HMWDSX3:3:1110:19090:84062209Test_seq1689946022H129M=169254411**NM:i:1MD:Z:44G84MC:Z:151MAS:i:124XS:i:49SA:Z:Test_seq,52324,-,127S24M,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1110:19090:8406177Test_seq523240127S24M=169254117058**NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:24SA:Z:Test_seq,168994,+,22S129M,60,1;XA:Z:Test_seq,-154647,127S24M,0;Test_seq,+255573,24M127S,0;Test_seq,+38332,24M127S,0;Test_seq,-170380,127S24M,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1110:19090:8406113Test_seq16925460151M=52324-117058**NM:i:1MD:Z:14T136MC:Z:127S24MAS:i:146XS:i:82Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1110:18765:8531	Test_seq	169027	Test_seq	169502	+	-	UU	A00126:224:H7HMWDSX3:3:1110:18765:853197Test_seq16902760151M=169352476**NM:i:5MD:Z:14T23A57A37G1A14MC:Z:151MAS:i:126XS:i:61Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:18765:8531145Test_seq16935260151M=169027-476**NM:i:1MD:Z:115G35MC:Z:151MAS:i:146XS:i:101Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1110:23927:10676	Test_seq	166048	Test_seq	166495	+	-	UU	A00126:224:H7HMWDSX3:3:1110:23927:10676161Test_seq16604860151M=166345448**NM:i:1MD:Z:143C7MC:Z:151MAS:i:146XS:i:45Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:23927:1067681Test_seq16634560151M=166048-448**NM:i:6MD:Z:25G14T10T38A2T12C44MC:Z:151MAS:i:121XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1110:29161:13416	Test_seq	169352	Test_seq	169778	+	-	UU	A00126:224:H7HMWDSX3:3:1110:29161:1341697Test_seq16935260151M=169628427**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:66Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:29161:13416145Test_seq16962860151M=169352-427**NM:i:4MD:Z:0T34G54T28C31MC:Z:151MAS:i:135XS:i:62Yt:Z:UU
@@ -393,7 +393,7 @@
 A00126:224:H7HMWDSX3:3:1111:27254:10113	!	0	Test_seq	169321	-	+	NR	A00126:224:H7HMWDSX3:3:1111:27254:10113145Test_seq16962860119M32S=169321-426**NM:i:4MD:Z:0T11A22G70A12MC:Z:151MAS:i:103XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1111:27254:1011397Test_seq16932160151M=169628426**NM:i:1MD:Z:146G4MC:Z:119M32SAS:i:146XS:i:112Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1111:22200:10222	Test_seq	117403	Test_seq	117847	+	-	UU	A00126:224:H7HMWDSX3:3:1111:22200:10222161Test_seq11740360151M=117697445**NM:i:1MD:Z:148G2MC:Z:151MAS:i:148XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:22200:1022281Test_seq11769760151M=117403-445**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:17499:11537	Test_seq	171298	Test_seq	171659	+	-	UU	A00126:224:H7HMWDSX3:3:1111:17499:1153797Test_seq17129860151M=171509362**NM:i:3MD:Z:62G39G15T32MC:Z:151MAS:i:136XS:i:61Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:17499:11537145Test_seq17150960151M=171298-362**NM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:47Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1111:19994:11788	!	0	Test_seq	168429	-	+	MR	A00126:224:H7HMWDSX3:3:1111:19994:11788145Test_seq202637124S27M=168429148141**NM:i:1MD:Z:6G20MC:Z:151MAS:i:22XS:i:20SA:Z:Test_seq,168657,-,120M31S,60,3;XA:Z:Test_seq,-55966,131S20M,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1111:19994:117882193Test_seq16865760120M31H=168429-348**NM:i:3MD:Z:18C30T9T60MC:Z:151MAS:i:105XS:i:50SA:Z:Test_seq,20263,-,124S27M,7,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1111:19994:1178897Test_seq16842960151M=20263-148141**NM:i:1MD:Z:66T84MC:Z:124S27MAS:i:146XS:i:78Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1111:19994:11788	!	0	Test_seq	168429	-	+	MR	A00126:224:H7HMWDSX3:3:1111:19994:117882193Test_seq16865760120M31H=168429-348**NM:i:3MD:Z:18C30T9T60MC:Z:151MAS:i:105XS:i:50SA:Z:Test_seq,20263,-,124S27M,7,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1111:19994:11788145Test_seq202637124S27M=168429148141**NM:i:1MD:Z:6G20MC:Z:151MAS:i:22XS:i:20SA:Z:Test_seq,168657,-,120M31S,60,3;XA:Z:Test_seq,-55966,131S20M,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1111:19994:1178897Test_seq16842960151M=20263-148141**NM:i:1MD:Z:66T84MC:Z:124S27MAS:i:146XS:i:78Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1111:20690:11929	!	0	Test_seq	167597	-	-	NR	A00126:224:H7HMWDSX3:3:1111:20690:1192997Test_seq1672982124S50M1I76M=167447300**NM:i:3MD:Z:25A44A55MC:Z:151MAS:i:109XS:i:99XA:Z:Test_seq,-213611,74M1I52M24S,5;Test_seq,+400719,24S50M1I76M,7;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1111:20690:11929145Test_seq16744760151M=167298-300**NM:i:0MD:Z:151MC:Z:24S50M1I76MAS:i:151XS:i:101Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1111:24795:12649	!	0	Test_seq	168660	-	-	MU	A00126:224:H7HMWDSX3:3:1111:24795:12649177Test_seq21264925151M=168510-44140**NM:i:10MD:Z:8G8A5T1T18C0T17T8T0C51G25MC:Z:151MAS:i:101XS:i:86XA:Z:Test_seq,+168239,151M,13;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1111:24795:12649113Test_seq16851060151M=21264944140**NM:i:1MD:Z:126A24MC:Z:151MAS:i:146XS:i:78Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1111:8802:14732	Test_seq	170230	Test_seq	170661	+	-	UU	A00126:224:H7HMWDSX3:3:1111:8802:14732161Test_seq17023060151M=170511432**NM:i:1MD:Z:47T103MC:Z:151MAS:i:146XS:i:72Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:8802:1473281Test_seq17051160151M=170230-432**NM:i:2MD:Z:76C26T47MC:Z:151MAS:i:141XS:i:37Yt:Z:UU
@@ -417,14 +417,14 @@
 A00126:224:H7HMWDSX3:3:1111:9661:28714	Test_seq	125729	Test_seq	126089	+	-	UU	A00126:224:H7HMWDSX3:3:1111:9661:2871497Test_seq12572960151M=125939361**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:9661:28714145Test_seq12593960151M=125729-361**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:19542:29011	!	0	Test_seq	171324	-	-	MU	A00126:224:H7HMWDSX3:3:1111:19542:2901197Test_seq1709453998M1D53M=171174380**NM:i:16MD:Z:8C5A2C8C2T0C7A0G5G22A19C0T1C6^T3C31T17MC:Z:151MAS:i:69XS:i:40Yt:Z:MU	A00126:224:H7HMWDSX3:3:1111:19542:29011145Test_seq17117460151M=170945-380**NM:i:0MD:Z:151MC:Z:98M1D53MAS:i:151XS:i:37Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1111:27706:33755	Test_seq	138290	Test_seq	138706	+	-	UU	A00126:224:H7HMWDSX3:3:1111:27706:3375597Test_seq13829060151M=138556417**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:27706:33755145Test_seq13855660151M=138290-417**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1111:8910:34209	!	0	Test_seq	167531	-	+	Mu	A00126:224:H7HMWDSX3:3:1111:8910:34209145Test_seq16755832151M=167531-178**NM:i:0MD:Z:151MC:Z:31S120MAS:i:151XS:i:136XA:Z:Test_seq,+213330,130M2I19M,3;Yt:Z:Mu	A00126:224:H7HMWDSX3:3:1111:8910:3420997Test_seq1675315931S120M=167558178**NM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:95Yt:Z:Mu
+A00126:224:H7HMWDSX3:3:1111:8910:34209	!	0	Test_seq	167531	-	+	MU	A00126:224:H7HMWDSX3:3:1111:8910:34209145Test_seq16755832151M=167531-178**NM:i:0MD:Z:151MC:Z:31S120MAS:i:151XS:i:136XA:Z:Test_seq,+213330,130M2I19M,3;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1111:8910:3420997Test_seq1675315931S120M=167558178**NM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:95Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1111:24777:34413	Test_seq	417145	Test_seq	417607	+	-	UU	A00126:224:H7HMWDSX3:3:1111:24777:3441397Test_seq41714560151M=417457463**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:24777:34413145Test_seq41745760151M=417145-463**NM:i:1MD:Z:46T104MC:Z:151MAS:i:146XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:27832:34663	!	0	Test_seq	167854	-	+	MU	A00126:224:H7HMWDSX3:3:1111:27832:34663145Test_seq16819737151M=167854-494**NM:i:4MD:Z:74T64C1T7G1MC:Z:151MAS:i:134XS:i:115XA:Z:Test_seq,+212691,151M,8;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1111:27832:3466397Test_seq16785460151M=168197494**NM:i:1MD:Z:59C91MC:Z:151MAS:i:146XS:i:59Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1111:8802:34773	!	0	Test_seq	168590	-	-	NR	A00126:224:H7HMWDSX3:3:1111:8802:34773161Test_seq1683476029S122M=168440244**NM:i:2MD:Z:18A28T74MC:Z:151MAS:i:112XS:i:66Yt:Z:NR	A00126:224:H7HMWDSX3:3:1111:8802:3477381Test_seq16844060151M=168347-244**NM:i:0MD:Z:151MC:Z:29S122MAS:i:151XS:i:83Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1111:1570:34773	Test_seq	72280	Test_seq	72638	+	-	UU	A00126:224:H7HMWDSX3:3:1111:1570:3477397Test_seq7228060151M=72488359**NM:i:1MD:Z:137A13MC:Z:151MAS:i:146XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:1570:34773145Test_seq7248860151M=72280-359**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:14064:36808	!	0	Test_seq	167148	-	+	MU	A00126:224:H7HMWDSX3:3:1111:14064:36808145Test_seq4009004135M16S=167148-233887**NM:i:7MD:Z:4T0T11G32G19A0C8T54MC:Z:151MAS:i:104XS:i:100XA:Z:Test_seq,-167437,135M16S,7;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1111:14064:3680897Test_seq16714842151M=400900233887**NM:i:1MD:Z:95T55MC:Z:135M16SAS:i:146XS:i:126XA:Z:Test_seq,-213736,151M,5;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1112:23149:2597	!	0	Test_seq	169468	-	-	MU	A00126:224:H7HMWDSX3:3:1112:23149:259797Test_seq1690793739M4I108M=169318390**NM:i:12MD:Z:40T3A37G1A37C11A1G2G7MC:Z:151MAS:i:99XS:i:76Yt:Z:MU	A00126:224:H7HMWDSX3:3:1112:23149:2597145Test_seq16931860151M=169079-390**NM:i:1MD:Z:149G1MC:Z:39M4I108MAS:i:149XS:i:112Yt:Z:MU
-A00126:224:H7HMWDSX3:3:1112:10447:5243	Test_seq	168427	Test_seq	168802	+	-	RU	A00126:224:H7HMWDSX3:3:1112:10447:5243161Test_seq16842760151M=168773376**NM:i:1MD:Z:68T82MC:Z:121S30MAS:i:146XS:i:43Yt:Z:RU	A00126:224:H7HMWDSX3:3:1112:10447:524381Test_seq16877360121S30M=168427-376**NM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,168555,-,121M30S,60,1;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1112:10447:52432129Test_seq16855560121M30H=168427-249**NM:i:1MD:Z:25A95MC:Z:151MAS:i:116XS:i:70SA:Z:Test_seq,168773,-,121S30M,60,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1112:10447:5243	Test_seq	168427	Test_seq	168802	+	-	RU	A00126:224:H7HMWDSX3:3:1112:10447:5243161Test_seq16842760151M=168773376**NM:i:1MD:Z:68T82MC:Z:121S30MAS:i:146XS:i:43Yt:Z:RU	A00126:224:H7HMWDSX3:3:1112:10447:52432129Test_seq16855560121M30H=168427-249**NM:i:1MD:Z:25A95MC:Z:151MAS:i:116XS:i:70SA:Z:Test_seq,168773,-,121S30M,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1112:10447:524381Test_seq16877360121S30M=168427-376**NM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,168555,-,121M30S,60,1;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1112:21884:7012	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1112:21884:701297Test_seq16797726151M=168170344**NM:i:8MD:Z:9T64G8C32T2C4T3A13G8MC:Z:151MAS:i:111XS:i:96XA:Z:Test_seq,-212911,141M10S,9;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1112:21884:7012145Test_seq16817031151M=167977-344**NM:i:1MD:Z:101T49MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,+212718,151M,4;Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1112:16595:8124	Test_seq	170144	Test_seq	170457	+	-	UU	A00126:224:H7HMWDSX3:3:1112:16595:8124161Test_seq1701446016S135M=170307314**NM:i:3MD:Z:51C0A65A16MC:Z:151MAS:i:120XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:16595:812481Test_seq17030760151M=170144-314**NM:i:1MD:Z:16A134MC:Z:16S135MAS:i:146XS:i:48Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:9209:9361	Test_seq	212679	Test_seq	212953	+	-	UU	A00126:224:H7HMWDSX3:3:1112:9209:936197Test_seq21267960151M=212803275**NM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:110Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:9209:9361145Test_seq21280358151M=212679-275**NM:i:5MD:Z:34A37T54T7T14C0MC:Z:151MAS:i:130XS:i:100Yt:Z:UU
@@ -436,7 +436,7 @@
 A00126:224:H7HMWDSX3:3:1112:5927:14763	Test_seq	366670	Test_seq	367055	+	-	UU	A00126:224:H7HMWDSX3:3:1112:5927:1476397Test_seq36667060151M=366905386**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:50Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:5927:14763145Test_seq36690560151M=366670-386**NM:i:1MD:Z:61G89MC:Z:151MAS:i:146XS:i:22Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:9254:15922	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1112:9254:1592297Test_seq260973853M=26097353**NM:i:3MD:Z:37G7C0T6MC:Z:53MAS:i:40XS:i:36XA:Z:Test_seq,+347640,45M8S,2;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1112:9254:15922145Test_seq2609733453M=260973-53**NM:i:0MD:Z:53MC:Z:53MAS:i:53XS:i:43XA:Z:Test_seq,-150325,53M,2;Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1112:32723:16391	!	0	Test_seq	171881	-	-	NR	A00126:224:H7HMWDSX3:3:1112:32723:16391161Test_seq1716266027S124M=171731256**NM:i:4MD:Z:50A14C13C11T32MC:Z:151MAS:i:104XS:i:71Yt:Z:NR	A00126:224:H7HMWDSX3:3:1112:32723:1639181Test_seq17173160151M=171626-256**NM:i:1MD:Z:27G123MC:Z:27S124MAS:i:146XS:i:25Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1112:27633:17722	!	0	Test_seq	388748	-	-	MR	A00126:224:H7HMWDSX3:3:1112:27633:17722113Test_seq38565530127S24M=3885983071**NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,388349,+,24S127M,60,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1112:27633:177222145Test_seq3883496024H127M=388598400**NM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0SA:Z:Test_seq,385655,-,127S24M,30,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1112:27633:17722177Test_seq38859860151M=385655-3071**NM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1112:27633:17722	!	0	Test_seq	388748	-	-	MR	A00126:224:H7HMWDSX3:3:1112:27633:177222145Test_seq3883496024H127M=388598400**NM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0SA:Z:Test_seq,385655,-,127S24M,30,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1112:27633:17722113Test_seq38565530127S24M=3885983071**NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,388349,+,24S127M,60,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1112:27633:17722177Test_seq38859860151M=385655-3071**NM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1112:25825:18098	!	0	Test_seq	171611	-	-	NR	A00126:224:H7HMWDSX3:3:1112:25825:18098161Test_seq1711746033S118M=171461438**NM:i:1MD:Z:23C94MC:Z:151MAS:i:113XS:i:35Yt:Z:NR	A00126:224:H7HMWDSX3:3:1112:25825:1809881Test_seq17146160151M=171174-438**NM:i:1MD:Z:122T28MC:Z:33S118MAS:i:146XS:i:39Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1112:9362:18740	Test_seq	170285	Test_seq	170742	+	-	UU	A00126:224:H7HMWDSX3:3:1112:9362:18740161Test_seq17028560151M=170592458**NM:i:1MD:Z:107T43MC:Z:151MAS:i:146XS:i:82Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:9362:1874081Test_seq17059260151M=170285-458**NM:i:8MD:Z:1G45A42G2G3A2A28A9G11MC:Z:151MAS:i:114XS:i:24Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:31421:19304	Test_seq	236629	Test_seq	237126	+	-	UU	A00126:224:H7HMWDSX3:3:1112:31421:1930497Test_seq23662960151M=236976498**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:31421:19304145Test_seq23697660151M=236629-498**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:28Yt:Z:UU
@@ -463,14 +463,14 @@
 A00126:224:H7HMWDSX3:3:1112:23285:36213	Test_seq	170144	Test_seq	170578	+	-	UU	A00126:224:H7HMWDSX3:3:1112:23285:3621397Test_seq17014460151M=170428435**NM:i:4MD:Z:25A25C0A65A32MC:Z:151MAS:i:131XS:i:59Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:23285:36213145Test_seq17042860151M=170144-435**NM:i:1MD:Z:62T88MC:Z:151MAS:i:146XS:i:25Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:27986:36777	!	0	Test_seq	213955	-	-	MU	A00126:224:H7HMWDSX3:3:1112:27986:36777113Test_seq1673130151M=21380546493**NM:i:4MD:Z:10A7T17T104A9MC:Z:151MAS:i:131XS:i:131XA:Z:Test_seq,+213571,151M,4;Test_seq,-400734,126M25S,4;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1112:27986:36777177Test_seq21380544151M=167313-46493**NM:i:1MD:Z:86G64MC:Z:151MAS:i:146XS:i:125XA:Z:Test_seq,+167079,151M,6;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1112:24695:36714	Test_seq	169001	Test_seq	169460	+	-	UU	A00126:224:H7HMWDSX3:3:1112:24695:36714161Test_seq1690016017S134M=169310460**NM:i:4MD:Z:35C1G26A31T37MC:Z:151MAS:i:114XS:i:60Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:24695:3671481Test_seq16931060151M=169001-460**NM:i:0MD:Z:151MC:Z:17S134MAS:i:151XS:i:110Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1112:22589:36886	!	0	Test_seq	167532	-	+	Mu	A00126:224:H7HMWDSX3:3:1112:22589:3688665Test_seq2131496151M=167532-45618**NM:i:1MD:Z:0T150MC:Z:28S123MAS:i:150XS:i:146XA:Z:Test_seq,-167739,151M,1;Yt:Z:Mu	A00126:224:H7HMWDSX3:3:1112:22589:36886129Test_seq1675324228S123M=21314945618**NM:i:3MD:Z:10G32T46T32MC:Z:151MAS:i:108XS:i:88XA:Z:Test_seq,+400995,28S118M5S,6;Yt:Z:Mu
+A00126:224:H7HMWDSX3:3:1112:22589:36886	!	0	Test_seq	167532	-	+	MU	A00126:224:H7HMWDSX3:3:1112:22589:3688665Test_seq2131496151M=167532-45618**NM:i:1MD:Z:0T150MC:Z:28S123MAS:i:150XS:i:146XA:Z:Test_seq,-167739,151M,1;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1112:22589:36886129Test_seq1675324228S123M=21314945618**NM:i:3MD:Z:10G32T46T32MC:Z:151MAS:i:108XS:i:88XA:Z:Test_seq,+400995,28S118M5S,6;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1113:12011:1908	Test_seq	170321	Test_seq	170762	+	-	UU	A00126:224:H7HMWDSX3:3:1113:12011:1908161Test_seq17032160151M=170612442**NM:i:1MD:Z:56G94MC:Z:151MAS:i:146XS:i:54Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:12011:190881Test_seq17061260151M=170321-442**NM:i:6MD:Z:73G6A38G1A9T4T14MC:Z:151MAS:i:121XS:i:37Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1113:24659:2049	Test_seq	362109	Test_seq	362498	+	-	UU	A00126:224:H7HMWDSX3:3:1113:24659:204997Test_seq36210960151M=362364390**NM:i:0MD:Z:151MC:Z:135M16SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:24659:2049145Test_seq36236460135M16S=362109-390**NM:i:0MD:Z:135MC:Z:151MAS:i:135XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1113:13123:3364	Test_seq	168222	Test_seq	168674	+	-	UU	A00126:224:H7HMWDSX3:3:1113:13123:3364161Test_seq16822243151M=168524453**NM:i:4MD:Z:49T37T36G18A7MC:Z:151MAS:i:131XS:i:109XA:Z:Test_seq,-212673,7S144M,7;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:13123:336481Test_seq16852460151M=168222-453**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1113:4526:3975	!	0	Test_seq	168585	-	-	MU	A00126:224:H7HMWDSX3:3:1113:4526:3975177Test_seq21273320151M=168435-44299**NM:i:3MD:Z:41G51A10A46MC:Z:151MAS:i:136XS:i:126XA:Z:Test_seq,+168155,151M,5;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1113:4526:3975113Test_seq16843560151M=21273344299**NM:i:1MD:Z:7T143MC:Z:151MAS:i:146XS:i:78Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1113:5565:7560	!	0	Test_seq	166317	-	+	NR	A00126:224:H7HMWDSX3:3:1113:5565:756081Test_seq16643460120M31S=166317-237**NM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:30Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:5565:7560161Test_seq16631760151M=166434237**NM:i:1MD:Z:49G101MC:Z:120M31SAS:i:146XS:i:32Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:12644:7701	Test_seq	424202	Test_seq	424540	+	-	UU	A00126:224:H7HMWDSX3:3:1113:12644:770197Test_seq42420260151M=424407339**NM:i:0MD:Z:151MC:Z:134M17SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:12644:7701145Test_seq42440760134M17S=424202-339**NM:i:0MD:Z:134MC:Z:151MAS:i:134XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1113:13765:8735	!	0	Test_seq	167972	-	-	Mu	A00126:224:H7HMWDSX3:3:1113:13765:8735129Test_seq16756832151M=328153160586**NM:i:0MD:Z:151MC:Z:2S25M124SAS:i:151XS:i:136XA:Z:Test_seq,-213320,140M2I9M,3;Yt:Z:Mu	A00126:224:H7HMWDSX3:3:1113:13765:873565Test_seq328153192S25M124S=167568-160586**NM:i:0MD:Z:25MC:Z:151MAS:i:25XS:i:21SA:Z:Test_seq,167849,-,124M27S,60,1;XA:Z:Test_seq,+141607,26M125S,1;Test_seq,+432045,26M125S,1;Yt:Z:MuNEXT_SAMA00126:224:H7HMWDSX3:3:1113:13765:87352129Test_seq16784960124M27H=167568-405**NM:i:1MD:Z:115T8MC:Z:151MAS:i:119XS:i:75SA:Z:Test_seq,328153,+,2S25M124S,19,0;Yt:Z:Mu
+A00126:224:H7HMWDSX3:3:1113:13765:8735	!	0	Test_seq	167972	-	-	MU	A00126:224:H7HMWDSX3:3:1113:13765:8735129Test_seq16756832151M=328153160586**NM:i:0MD:Z:151MC:Z:2S25M124SAS:i:151XS:i:136XA:Z:Test_seq,-213320,140M2I9M,3;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1113:13765:87352129Test_seq16784960124M27H=167568-405**NM:i:1MD:Z:115T8MC:Z:151MAS:i:119XS:i:75SA:Z:Test_seq,328153,+,2S25M124S,19,0;Yt:Z:MUNEXT_SAMA00126:224:H7HMWDSX3:3:1113:13765:873565Test_seq328153192S25M124S=167568-160586**NM:i:0MD:Z:25MC:Z:151MAS:i:25XS:i:21SA:Z:Test_seq,167849,-,124M27S,60,1;XA:Z:Test_seq,+141607,26M125S,1;Test_seq,+432045,26M125S,1;Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1113:8892:9878	!	0	Test_seq	397096	-	-	NR	A00126:224:H7HMWDSX3:3:1113:8892:9878161Test_seq3967016025S126M=396946396**NM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:8892:987881Test_seq39694660151M=396701-396**NM:i:1MD:Z:95A55MC:Z:25S126MAS:i:146XS:i:58Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:7084:10441	!	0	Test_seq	167640	-	-	NR	A00126:224:H7HMWDSX3:3:1113:7084:10441161Test_seq167316721S130M=167490325**NM:i:3MD:Z:7A44A73T3MC:Z:151MAS:i:116XS:i:111XA:Z:Test_seq,-213589,130M21S,4;Test_seq,+400737,21S123M7S,5;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:7084:1044181Test_seq16749060151M=167316-325**NM:i:1MD:Z:102G48MC:Z:21S130MAS:i:146XS:i:96Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:3025:10614	Test_seq	385792	Test_seq	386184	+	-	UU	A00126:224:H7HMWDSX3:3:1113:3025:10614161Test_seq3857926015S136M=386034393**NM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:3025:1061481Test_seq38603460151M=385792-393**NM:i:0MD:Z:151MC:Z:15S136MAS:i:151XS:i:0Yt:Z:UU
@@ -505,11 +505,11 @@
 A00126:224:H7HMWDSX3:3:1113:23086:35149	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1113:23086:3514997Test_seq1702663960M=17026660**NM:i:0MD:Z:60MC:Z:60MAS:i:60XS:i:48Yt:Z:MM	A00126:224:H7HMWDSX3:3:1113:23086:35149145Test_seq1702663960M=170266-60**NM:i:0MD:Z:60MC:Z:60MAS:i:60XS:i:48Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1113:25057:36714	!	0	Test_seq	168557	-	-	MU	A00126:224:H7HMWDSX3:3:1113:25057:36714161Test_seq1682382415S136M=168407320**NM:i:6MD:Z:33T4G5T18T44G18A8MC:Z:151MAS:i:106XS:i:93XA:Z:Test_seq,-212673,8S128M15S,7;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1113:25057:3671481Test_seq16840760151M=168238-320**NM:i:1MD:Z:88T62MC:Z:15S136MAS:i:146XS:i:78Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1114:11785:1485	Test_seq	170257	Test_seq	170704	+	-	UU	A00126:224:H7HMWDSX3:3:1114:11785:148597Test_seq17025760151M=170566448**NM:i:1MD:Z:5A145MC:Z:32M12I107MAS:i:146XS:i:80Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:11785:1485145Test_seq1705666032M12I107M=170257-448**NM:i:20MD:Z:2A10T32A3A3T64G6A8C3MC:Z:151MAS:i:84XS:i:37Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1114:14986:3239	!	0	Test_seq	333500	-	-	Mu	A00126:224:H7HMWDSX3:3:1114:14986:3239161Test_seq33315332151M=333377348**NM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:136XA:Z:Test_seq,+315878,151M,3;Yt:Z:Mu	A00126:224:H7HMWDSX3:3:1114:14986:323981Test_seq33337760124M27S=333153-348**NM:i:1MD:Z:0A123MC:Z:151MAS:i:123XS:i:62Yt:Z:Mu
+A00126:224:H7HMWDSX3:3:1114:14986:3239	!	0	Test_seq	333500	-	-	MU	A00126:224:H7HMWDSX3:3:1114:14986:3239161Test_seq33315332151M=333377348**NM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:136XA:Z:Test_seq,+315878,151M,3;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1114:14986:323981Test_seq33337760124M27S=333153-348**NM:i:1MD:Z:0A123MC:Z:151MAS:i:123XS:i:62Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1114:26268:3333	Test_seq	435684	Test_seq	436086	+	-	UU	A00126:224:H7HMWDSX3:3:1114:26268:3333161Test_seq43568460151M=435936403**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:31Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:26268:333381Test_seq43593660151M=435684-403**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1114:5791:5008	!	0	Test_seq	71380	-	-	MR	A00126:224:H7HMWDSX3:3:1114:5791:5008113Test_seq703106131S20M=712301052**NM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,71226,+,20S131M,60,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1114:5791:50082145Test_seq712266020H131M=71230155**NM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:0SA:Z:Test_seq,70310,-,131S20M,6,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1114:5791:5008177Test_seq7123060151M=70310-1052**NM:i:0MD:Z:151MC:Z:131S20MAS:i:151XS:i:0Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1114:5791:5008	!	0	Test_seq	71380	-	-	MR	A00126:224:H7HMWDSX3:3:1114:5791:50082145Test_seq712266020H131M=71230155**NM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:0SA:Z:Test_seq,70310,-,131S20M,6,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1114:5791:5008113Test_seq703106131S20M=712301052**NM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,71226,+,20S131M,60,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1114:5791:5008177Test_seq7123060151M=70310-1052**NM:i:0MD:Z:151MC:Z:131S20MAS:i:151XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1114:24153:6778	Test_seq	166090	Test_seq	166553	+	-	UU	A00126:224:H7HMWDSX3:3:1114:24153:677897Test_seq16609060151M=166418464**NM:i:1MD:Z:134G16MC:Z:136M15SAS:i:146XS:i:85Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:24153:6778145Test_seq16641860136M15S=166090-464**NM:i:4MD:Z:39C23A2A20T48MC:Z:151MAS:i:116XS:i:40Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1114:29423:7827	!	0	Test_seq	168477	-	+	MR	A00126:224:H7HMWDSX3:3:1114:29423:7827145Test_seq158736131S20M=168477152586**NM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,168685,-,126M25S,60,2;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1114:29423:78272193Test_seq16868560126M25H=168477-334**NM:i:2MD:Z:2A28T94MC:Z:151MAS:i:118XS:i:58SA:Z:Test_seq,15873,-,131S20M,6,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1114:29423:782797Test_seq16847760151M=15873-152586**NM:i:1MD:Z:18T132MC:Z:131S20MAS:i:146XS:i:92Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1114:29423:7827	!	0	Test_seq	168477	-	+	MR	A00126:224:H7HMWDSX3:3:1114:29423:78272193Test_seq16868560126M25H=168477-334**NM:i:2MD:Z:2A28T94MC:Z:151MAS:i:118XS:i:58SA:Z:Test_seq,15873,-,131S20M,6,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1114:29423:7827145Test_seq158736131S20M=168477152586**NM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,168685,-,126M25S,60,2;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1114:29423:782797Test_seq16847760151M=15873-152586**NM:i:1MD:Z:18T132MC:Z:131S20MAS:i:146XS:i:92Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1114:22453:9032	Test_seq	414176	Test_seq	414453	+	-	UU	A00126:224:H7HMWDSX3:3:1114:22453:903297Test_seq41417660151M=414303278**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:22453:9032145Test_seq41430360151M=414176-278**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:27959:9392	Test_seq	171239	Test_seq	171657	+	-	UU	A00126:224:H7HMWDSX3:3:1114:27959:939297Test_seq17123960151M=171507419**NM:i:1MD:Z:63G87MC:Z:151MAS:i:146XS:i:65Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:27959:9392145Test_seq17150760151M=171239-419**NM:i:3MD:Z:17G46C11T74MC:Z:151MAS:i:136XS:i:47Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:13259:9518	Test_seq	305947	Test_seq	306239	+	-	UU	A00126:224:H7HMWDSX3:3:1114:13259:9518161Test_seq30594760151M=306104293**NM:i:0MD:Z:151MC:Z:136M15SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:13259:951881Test_seq30610460136M15S=305947-293**NM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:0Yt:Z:UU
@@ -539,7 +539,7 @@
 A00126:224:H7HMWDSX3:3:1114:6424:26209	Test_seq	166060	Test_seq	166536	+	-	UU	A00126:224:H7HMWDSX3:3:1114:6424:2620997Test_seq16606060151M=166386477**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:57Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:6424:26209145Test_seq16638660151M=166060-477**NM:i:7MD:Z:10T1T5A4C56T4G33T31MC:Z:151MAS:i:116XS:i:32Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:14534:27164	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1114:14534:2716481Test_seq1534781254M=153478-54**NM:i:2MD:Z:38G8T6MC:Z:54MAS:i:44XS:i:39XA:Z:Test_seq,-51125,54M,3;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1114:14534:27164161Test_seq1534781354M=15347854**NM:i:1MD:Z:18T35MC:Z:54MAS:i:49XS:i:44XA:Z:Test_seq,+51125,54M,2;Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1114:23936:27602	Test_seq	169575	Test_seq	169995	+	-	UU	A00126:224:H7HMWDSX3:3:1114:23936:27602161Test_seq16957560151M=169843421**NM:i:1MD:Z:88G62MC:Z:34M1D35M1D82MAS:i:146XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:23936:2760281Test_seq1698436034M1D35M1D82M=169575-421**NM:i:5MD:Z:23C10^A35^T12T47G21MC:Z:151MAS:i:122XS:i:47Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1114:5864:28119	!	0	Test_seq	168029	-	-	Mu	A00126:224:H7HMWDSX3:3:1114:5864:2811997Test_seq16756831151M=167902462**NM:i:1MD:Z:144T6MC:Z:128M23SAS:i:146XS:i:131XA:Z:Test_seq,-213320,140M2I9M,4;Yt:Z:Mu	A00126:224:H7HMWDSX3:3:1114:5864:28119145Test_seq16790260128M23S=167568-462**NM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0Yt:Z:Mu
+A00126:224:H7HMWDSX3:3:1114:5864:28119	!	0	Test_seq	168029	-	-	MU	A00126:224:H7HMWDSX3:3:1114:5864:2811997Test_seq16756831151M=167902462**NM:i:1MD:Z:144T6MC:Z:128M23SAS:i:146XS:i:131XA:Z:Test_seq,-213320,140M2I9M,4;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1114:5864:28119145Test_seq16790260128M23S=167568-462**NM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1114:28212:28808	Test_seq	168997	Test_seq	169468	+	-	UU	A00126:224:H7HMWDSX3:3:1114:28212:2880897Test_seq16899760151M=169318472**NM:i:6MD:Z:2G11G0G28T23A57A24MC:Z:151MAS:i:123XS:i:65Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:28212:28808145Test_seq16931860151M=168997-472**NM:i:1MD:Z:149G1MC:Z:151MAS:i:149XS:i:112Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:10176:29168	Test_seq	161012	Test_seq	161354	+	-	UU	A00126:224:H7HMWDSX3:3:1114:10176:29168161Test_seq16101260151M=161204343**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:51Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:10176:2916881Test_seq16120460151M=161012-343**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:28266:30436	Test_seq	168757	Test_seq	169067	+	-	UU	A00126:224:H7HMWDSX3:3:1114:28266:3043697Test_seq16875760151M=168917311**NM:i:1MD:Z:20A130MC:Z:151MAS:i:146XS:i:82Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:28266:30436145Test_seq16891760151M=168757-311**NM:i:5MD:Z:7G11T22G48G29G29MC:Z:151MAS:i:126XS:i:67Yt:Z:UU
@@ -574,7 +574,7 @@
 A00126:224:H7HMWDSX3:3:1115:1967:25974	!	0	Test_seq	167599	-	-	MU	A00126:224:H7HMWDSX3:3:1115:1967:2597497Test_seq16715018151M=167449450**NM:i:6MD:Z:28A23G22T15A1T49G7MC:Z:151MAS:i:121XS:i:111XA:Z:Test_seq,-213734,151M,8;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1115:1967:25974145Test_seq16744960151M=167150-450**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:101Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1115:7789:25974	!	0	Test_seq	167947	-	-	NR	A00126:224:H7HMWDSX3:3:1115:7789:25974161Test_seq1675683432S119M=167797380**NM:i:1MD:Z:76T42MC:Z:151MAS:i:114XS:i:99XA:Z:Test_seq,-213352,108M2I9M32S,4;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1115:7789:2597481Test_seq16779760151M=167568-380**NM:i:1MD:Z:21C129MC:Z:32S119MAS:i:146XS:i:113Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1115:18032:26678	Test_seq	170112	Test_seq	170470	+	-	UU	A00126:224:H7HMWDSX3:3:1115:18032:26678161Test_seq17011260151M=170320359**NM:i:3MD:Z:3G20A59A66MC:Z:151MAS:i:137XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:18032:2667881Test_seq17032060151M=170112-359**NM:i:1MD:Z:56C94MC:Z:151MAS:i:146XS:i:48Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1115:6686:27383	Test_seq	166310	Test_seq	166674	+	-	RU	A00126:224:H7HMWDSX3:3:1115:6686:2738397Test_seq16631060151M=166550365**NM:i:1MD:Z:6T144MC:Z:26S125MAS:i:146XS:i:27Yt:Z:RU	A00126:224:H7HMWDSX3:3:1115:6686:27383145Test_seq1665506026S125M=166310-365**NM:i:3MD:Z:27C50T9C36MC:Z:151MAS:i:110XS:i:63SA:Z:Test_seq,166445,-,27M124S,48,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1115:6686:273832193Test_seq1664454827M124H=166310-162**NM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,166550,-,26S125M,60,3;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1115:6686:27383	Test_seq	166310	Test_seq	166674	+	-	RU	A00126:224:H7HMWDSX3:3:1115:6686:2738397Test_seq16631060151M=166550365**NM:i:1MD:Z:6T144MC:Z:26S125MAS:i:146XS:i:27Yt:Z:RU	A00126:224:H7HMWDSX3:3:1115:6686:273832193Test_seq1664454827M124H=166310-162**NM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,166550,-,26S125M,60,3;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1115:6686:27383145Test_seq1665506026S125M=166310-365**NM:i:3MD:Z:27C50T9C36MC:Z:151MAS:i:110XS:i:63SA:Z:Test_seq,166445,-,27M124S,48,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1115:27516:27915	Test_seq	169316	Test_seq	169778	+	-	UU	A00126:224:H7HMWDSX3:3:1115:27516:27915161Test_seq16931660151M=169628463**NM:i:1MD:Z:37T113MC:Z:151MAS:i:146XS:i:107Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:27516:2791581Test_seq16962860151M=169316-463**NM:i:8MD:Z:0T5T5A22G83C4T5C2C17MC:Z:151MAS:i:115XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1115:31584:28040	!	0	Test_seq	167925	-	+	NR	A00126:224:H7HMWDSX3:3:1115:31584:2804081Test_seq16821860128M23S=167925-421**NM:i:4MD:Z:26A26T27G11T34MC:Z:151MAS:i:108XS:i:31Yt:Z:NR	A00126:224:H7HMWDSX3:3:1115:31584:28040161Test_seq16792560151M=168218421**NM:i:1MD:Z:135C15MC:Z:128M23SAS:i:146XS:i:73Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1115:15944:29731	!	0	Test_seq	167319	-	-	MU	A00126:224:H7HMWDSX3:3:1115:15944:2973197Test_seq16686738151M=167169453**NM:i:5MD:Z:26G9G13T10A8T80MC:Z:151MAS:i:126XS:i:106XA:Z:Test_seq,-214017,151M,9;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1115:15944:29731145Test_seq16716952151M=166867-453**NM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213715,151M,6;Yt:Z:MU
@@ -590,7 +590,7 @@
 A00126:224:H7HMWDSX3:3:1116:21097:1329	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1116:21097:132965Test_seq16796621151M=21271844753**NM:i:11MD:Z:7T4G3G2A35T29G8C34G0C4T3A11MC:Z:151MAS:i:96XS:i:83XA:Z:Test_seq,-212922,151M,14;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1116:21097:1329129Test_seq21271831151M=167966-44753**NM:i:1MD:Z:108A42MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,-168170,151M,4;Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1116:9525:1548	Test_seq	165792	Test_seq	166199	+	-	UU	A00126:224:H7HMWDSX3:3:1116:9525:1548161Test_seq16579260110M2D41M=166049408**NM:i:12MD:Z:2A5T9T13C31A10A8G11A13^CC7G19T13MC:Z:151MAS:i:95XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:9525:154881Test_seq16604960151M=165792-408**NM:i:1MD:Z:53G97MC:Z:110M2D41MAS:i:146XS:i:50Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:28917:2190	Test_seq	74270	Test_seq	74579	+	-	UU	A00126:224:H7HMWDSX3:3:1116:28917:219097Test_seq7427060151M=74429310**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:41Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:28917:2190145Test_seq7442960151M=74270-310**NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1116:3495:3724	Test_seq	335700	Test_seq	436771	-	-	UR	A00126:224:H7HMWDSX3:3:1116:3495:3724177Test_seq33566960119S32M=436621101072**NM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,436401,+,32S119M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1116:3495:37242209Test_seq4364016032H119M=436621371**NM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,335669,-,119S32M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1116:3495:3724113Test_seq43662160151M=335669-101072**NM:i:0MD:Z:151MC:Z:119S32MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1116:3495:3724	Test_seq	335700	Test_seq	436771	-	-	UR	A00126:224:H7HMWDSX3:3:1116:3495:37242209Test_seq4364016032H119M=436621371**NM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,335669,-,119S32M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1116:3495:3724177Test_seq33566960119S32M=436621101072**NM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,436401,+,32S119M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1116:3495:3724113Test_seq43662160151M=335669-101072**NM:i:0MD:Z:151MC:Z:119S32MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1116:1886:5885	Test_seq	133235	Test_seq	133657	+	-	UU	A00126:224:H7HMWDSX3:3:1116:1886:588597Test_seq13323560151M=133507423**NM:i:1MD:Z:36C114MC:Z:151MAS:i:146XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:1886:5885145Test_seq13350760151M=133235-423**NM:i:5MD:Z:12G29T70C20C2C13MC:Z:151MAS:i:126XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:5041:6715	Test_seq	168462	Test_seq	168810	+	-	UU	A00126:224:H7HMWDSX3:3:1116:5041:671597Test_seq16846260151M=168674349**NM:i:1MD:Z:33T117MC:Z:137M14SAS:i:146XS:i:91Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:5041:6715145Test_seq16867460137M14S=168462-349**NM:i:5MD:Z:42T10T2C7A19G52MC:Z:151MAS:i:112XS:i:30Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:31720:7545	!	0	Test_seq	303522	-	+	MR	A00126:224:H7HMWDSX3:3:1116:31720:754565Test_seq305328620M131S=303522-1807**NM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,303836,-,131M20S,60,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1116:31720:75452129Test_seq30383660131M20H=303522-445**NM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:43SA:Z:Test_seq,305328,+,20M131S,6,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1116:31720:7545129Test_seq30352260151M=3053281807**NM:i:0MD:Z:151MC:Z:20M131SAS:i:151XS:i:0Yt:Z:MR
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_parsed_pairs_sam.pairs
--- a/test-data/output_parsed_pairs_sam.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_parsed_pairs_sam.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -4,13 +4,13 @@
 #chromsize: Test_seq 450060
 #samheader: @SQ	SN:Test_seq	LN:450060
 #samheader: @PG	ID:bwa-mem2	PN:bwa-mem2	VN:2.2.1	CL:bwa-mem2 mem -5SP -T0 -t 8 test test_hic_reads_1.fq test_hic_reads_2.fq
-#samheader: @PG	ID:pairtools_parse	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/1/f/1/dataset_1f1b27bb-444f-4f9a-b79d-0addfd198f84.dat -c /tmp/tmpvtvmbj0m/files/a/6/c/dataset_a6c25687-079e-4e5f-a858-96e64dab4aff.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/3/outputs/dataset_4f9f5c5c-4d65-4e66-9fd4-61c5ab9be509.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-mem2	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/2/e/1/dataset_2e1a2abe-d4ed-46d7-90b0-02f7ad21e679.dat -c /tmp/tmpchj83off/files/4/0/d/dataset_40dde3ed-dc87-49a5-b6d7-c9bb448a57ee.dat -o /tmp/tmpchj83off/job_working_directory/000/3/outputs/dataset_bb5fe9ed-7777-4b2b-88c3-9f89a9d6e0ce.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-mem2	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 A00126:224:H7HMWDSX3:3:1101:4779:3098	Test_seq	166211	Test_seq	214901	-	-	UU	A00126:224:H7HMWDSX3:3:1101:4779:3098113Test_seq1661344378M=21482448691GGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:78MC:Z:78MAS:i:78XS:i:63XA:Z:Test_seq,+214829,5S73M,2;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:4779:3098177Test_seq214824578M=166134-48691AAGAGCCCAAGAGACAGAAAGGGCCACATGAACCACAGACCTACATCATCCTGAGACCAGAAGAACTAGTTGGTGCCCFFFF,FFFFFFFFFFFFF,FFFFFFFFFFFF:FF:,FFFFFFF:FF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:3MD:Z:1T33G33A8MC:Z:78MAS:i:66XS:i:63XA:Z:Test_seq,+166134,78M,3;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:2908:3239	Test_seq	167331	Test_seq	167696	+	-	UU	A00126:224:H7HMWDSX3:3:1101:2908:3239161Test_seq16733123151M=167546366TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFF:FFFFF:FFFF:FFFFFFFFFFFFFFF:FF,,FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFF:FF,FFNM:i:1MD:Z:37A113MC:Z:151MAS:i:146XS:i:135XA:Z:Test_seq,-213553,151M,4;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:2908:323981Test_seq16754644151M=167331-366TAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTACAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACFF,F,F:FFFFFFFFF,FFF:FFFFFF:FFFF:FFFFFFFFFFF:FF::F,FFFFFFFFF:,F,FFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFF::FFFFFFFFFFFFF,FFFFFFF:FF:F,:FFFFFFFFFF,F,F:FFFFFFFFFFNM:i:3MD:Z:82G15T30C21MC:Z:151MAS:i:136XS:i:114XA:Z:Test_seq,+213342,118M2I23M2I6M,8;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:27208:4492	Test_seq	194620	Test_seq	194909	+	-	UU	A00126:224:H7HMWDSX3:3:1101:27208:449297Test_seq19462060151M=194778290GCTGTGCAAACCACTGCTCCAGGCTATCTCAGCACTGAGCCAGAGACAAGGGCCAGTTCAGTCACCTGGAAAATCAGGCCAGCATCTCTGAAAAATAGGTGCCTCAGTGTTCACAGAAAAGGGCCATGTATTGAAGTAGGTCAGAGGAAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,FF:::FFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:27208:4492145Test_seq19477860132M19S=194620-290CACTCACCAGATTCAGCCCAGGACCAGAGGCTGCCGAGGGCAGGTGTGGGACGAAGTCAAGATGAAGTCAGTTCATTGTGTGGTTTTCGGTACCAGGCTAGACCTAGATTTGAACCCCAAGTCTGCTACTTAGATCATTCATTCAATGTGTFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:9344:5306	Test_seq	449503	Test_seq	449562	+	-	UU	A00126:224:H7HMWDSX3:3:1101:9344:530697Test_seq449503760M=44950360GTTCGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTATGAGTCAGTGT,FFFF,FFF:FF,FF:FF:FF:F:,F::,FF:,FFFFFFFF::FF,,:FFF,FFF::,F,NM:i:4MD:Z:3T44G2C5G2MC:Z:60MAS:i:44XS:i:40XA:Z:Test_seq,+16926,60M,5;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:9344:5306145Test_seq4495031260M=449503-60GTTTGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTGTGCGCCAGGGTFFFFFFFFF:F::FF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:F:FFFFNM:i:1MD:Z:53T6MC:Z:60MAS:i:55XS:i:50XA:Z:Test_seq,-16926,60M,2;Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1101:17607:5212	Test_seq	83425	Test_seq	93852	-	+	UR	A00126:224:H7HMWDSX3:3:1101:17607:521281Test_seq8339760122S29M=9385210428GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATCAATCCAGCTGATTTTTGTGTGTTTACCTT:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,94155,-,122M29S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1101:17607:52122129Test_seq9415560122M29H=93852-425GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATC:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0SA:Z:Test_seq,83397,-,122S29M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1101:17607:5212161Test_seq9385260151M=83397-10428AAACATGGAACGGCAAAAAATGTGGCACCGACCCGGCTATATGAAGGACACTTGTTTACAGTGTGAGAGCTGAAACAAGAAGGCAGAGTGCGGCTTTGTTTGACCTCTGTTGGAAACGCGTGCATCGGGTGATTCAAAATTTCCACCACTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:122S29MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1101:17607:5212	Test_seq	83425	Test_seq	93852	-	+	UR	A00126:224:H7HMWDSX3:3:1101:17607:52122129Test_seq9415560122M29H=93852-425GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATC:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0SA:Z:Test_seq,83397,-,122S29M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1101:17607:521281Test_seq8339760122S29M=9385210428GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATCAATCCAGCTGATTTTTGTGTGTTTACCTT:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,94155,-,122M29S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1101:17607:5212161Test_seq9385260151M=83397-10428AAACATGGAACGGCAAAAAATGTGGCACCGACCCGGCTATATGAAGGACACTTGTTTACAGTGTGAGAGCTGAAACAAGAAGGCAGAGTGCGGCTTTGTTTGACCTCTGTTGGAAACGCGTGCATCGGGTGATTCAAAATTTCCACCACTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:122S29MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1101:17942:7263	Test_seq	168528	Test_seq	168867	+	-	UU	A00126:224:H7HMWDSX3:3:1101:17942:726397Test_seq16852860151M=168717340ATTTAGAGTGATGTTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTTTATCATAAATGGGTGCTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATGFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFF:FFFF,FFFFFFFFF:FFFFF,::FFFF::F:FFFNM:i:5MD:Z:12A68A23T2A26T15MC:Z:151MAS:i:126XS:i:91Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:17942:7263145Test_seq16871760151M=168528-340ATTAATGGTTTTTCTGATATTAAACCAACCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:27G123MC:Z:151MAS:i:146XS:i:91Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:5683:8108	!	0	Test_seq	170834	-	+	MR	A00126:224:H7HMWDSX3:3:1101:5683:810865Test_seq137032028M123S=17083433803GATTTTTATTTTCTTTATGTTGAGGTGTGATCGATCCAAAAAGAAAAACACCAAGACATATTATCATCAAACTTGCCAAAACCAAAGACAAACAGAAAATTTTAAAAGCAGCCAGGGAGAAAAGAAAGGTTTCCTTCAAGGGAGAATCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF:FFFFFNM:i:1MD:Z:7G20MC:Z:151MAS:i:23XS:i:23SA:Z:Test_seq,171170,-,119M32S,60,0;XA:Z:Test_seq,+333108,28M123S,1;Test_seq,-298750,123S26M2S,1;Test_seq,-279319,123S28M,2;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1101:5683:81082129Test_seq17117060119M32H=170834-455ATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFF:FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,137032,+,28M123S,0,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1101:5683:8108129Test_seq17083460151M=137032-33803ATCTTACTTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTAAGCATTTCTTGCAGTCTTGGTTTAGFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:28M123SAS:i:151XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1101:3947:9361	!	0	Test_seq	171174	-	+	NR	A00126:224:H7HMWDSX3:3:1101:3947:9361145Test_seq17134260129M22S=171174-297TGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTATAAAAATAAAATGCTTGCTTACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFNM:i:2MD:Z:58G15T54MC:Z:151MAS:i:119XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1101:3947:936197Test_seq17117460151M=171342297ATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGAFFFFFFFFFFFFFFFF,,:FF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFF:F:FFFFFFFF::FFFFFF:FFFFFFFF:FFFFF,FFFFFFFFFF,FFFFFFF:FFF,FNM:i:0MD:Z:151MC:Z:129M22SAS:i:151XS:i:37Yt:Z:NR
@@ -46,12 +46,12 @@
 A00126:224:H7HMWDSX3:3:1101:13042:33630	Test_seq	166363	Test_seq	166687	+	-	UU	A00126:224:H7HMWDSX3:3:1101:13042:3363097Test_seq16636360151M=166544325TTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF,F:FFF:FF:,FFF,FFFF:F,:FFFFFFFF::FF:F,NM:i:0MD:Z:151MC:Z:5M3I18M4I121MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:13042:33630145Test_seq166544605M3I18M4I121M=166363-325CTGATTGATGATCAATAAAAAACCCTCTCCCTCCCTCCCTCCCTCCCTCCCCCCTTTGTAACCACATAAGTATGTGTTCTTCTCCGTTTTTTCTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCTTTTGCCTCTGACF:,FFFFFFFFFFFFF:FFFFFF:F,FFFFFFFF:FF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFFF:F:FFFFFF:,FFFFF,FFFFFNM:i:9MD:Z:59A34C49MC:Z:151MAS:i:119XS:i:62Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:29966:36072	Test_seq	170104	Test_seq	170357	+	-	UU	A00126:224:H7HMWDSX3:3:1101:29966:36072161Test_seq17010460151M=170207254TCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFF:F:FFFFFFFFFFFFFF:,:FFFF:F:FFFFFFFFFFNM:i:6MD:Z:3C7G20A32A25C0A58MC:Z:151MAS:i:122XS:i:49Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:29966:3607281Test_seq17020760151M=170104-254TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:1MD:Z:55A95MC:Z:151MAS:i:146XS:i:88Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:3595:36620	Test_seq	167567	Test_seq	167976	+	-	UU	A00126:224:H7HMWDSX3:3:1101:3595:36620161Test_seq1675673015S136M=167826410ACCAACCAGCTAGACTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATTGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGATAAAAAGACTATCATTTCCCCAAATGACTGACACTGGGCCFFFFFFFF,,FF:FFFFFF,FF:FFFFF:FFFFF:FFFFF:FFFFFFFFFF:FFFF,FF:,,FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:,:F,FFFF:FFFFFFF:F:FFFFF,FF,F:FF,:FFFFFFFF::NM:i:4MD:Z:41G53T23T13T2MC:Z:151MAS:i:118XS:i:103XA:Z:Test_seq,-213336,124M2I10M15S,7;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:3595:3662081Test_seq16782660151M=167567-410ATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCACATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAFFFFFFFFFFFFF::FFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF::F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF,FFFFFFF:FF,FF:,FFFFFFFFFFFFFFFNM:i:1MD:Z:90T60MC:Z:15S136MAS:i:146XS:i:87Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1102:11306:1908	Test_seq	186004	Test_seq	256018	-	-	RU	A00126:224:H7HMWDSX3:3:1102:11306:1908177Test_seq18585460151M=25599370015ATGTGCCAGGTGCTGTTTTATGCTTTCATCTTCACTACTTCAGTCAGTCCTCACTGCAAACCAGTGAGGCAGATAGTATTATTCCCACTTTACAGTTGAAGAGACTGAGGCTCTATGAAATTAAGAAAATGTCCCCAGCCACAGGGCTAGTFFFFF:::F,F:FF:F:FFFF::FFF,FF,F,F:FFF:F:,F:FFFFFF:FFFFFF:FFF,:F::FFFF:FFFF:FFFFFFFFFFFFFFFFF:F:FF,FFFFFFF:FFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:31G119MC:Z:125S26MAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:11306:1908113Test_seq25599342125S26M=185854-70015AGTAGTATTTAGCTATTCTTTCCCTCATATTTTCCAACAATGTCATTTTGCTCTAATTTTTCAAAAGTGTCTTCTGCTTTATTTTCCAGCTTATTGATTTTTTTATATTAGTAATAATTTTGATCGATCTAAATGCCCAATCAACCAACTT,,FF,::F:F:,:FFF:,F:FF,FF,:F,FFFFF,F,F,FFFFFFFF:F,FF:F:F,FFFFFF::FFF,F,FFFFFFFFFFFFFF,,FFF:FFFFF,FFFFF:FFFFFFFFFFFF,FFFFFFFFF:FFFFF,F,F:FFFFFFFFFFFFFFFNM:i:0MD:Z:26MC:Z:151MAS:i:26XS:i:0SA:Z:Test_seq,185684,+,26S125M,60,3;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:11306:19082145Test_seq1856846026H125M=185854321GATCAAAATTATTACTAATATAAAAAAATCAATAAGCTGGAAAATAAAGCAGAAGACACTTTTGAAAAATTAGAGCAAAATGACATTGTTGGAAAATATGAGGGAAAGAATAGCTAAATACTACTFFFFFFFFF,FFFFFFFFFFFF:FFFFF,FFFFF:FFF,,FFFFFFFFFFFFFF,F,FFF::FFFFFF,F:F:FF,F:FFFFFFFF,F,F,FFFFF,F:,FF,FF:F,:FFF:,:F:F::,FF,,NM:i:3MD:Z:68T17G1G36MC:Z:151MAS:i:110XS:i:0SA:Z:Test_seq,255993,-,125S26M,42,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:11306:1908	Test_seq	186004	Test_seq	256018	-	-	RU	A00126:224:H7HMWDSX3:3:1102:11306:1908177Test_seq18585460151M=25599370015ATGTGCCAGGTGCTGTTTTATGCTTTCATCTTCACTACTTCAGTCAGTCCTCACTGCAAACCAGTGAGGCAGATAGTATTATTCCCACTTTACAGTTGAAGAGACTGAGGCTCTATGAAATTAAGAAAATGTCCCCAGCCACAGGGCTAGTFFFFF:::F,F:FF:F:FFFF::FFF,FF,F,F:FFF:F:,F:FFFFFF:FFFFFF:FFF,:F::FFFF:FFFF:FFFFFFFFFFFFFFFFF:F:FF,FFFFFFF:FFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:31G119MC:Z:125S26MAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:11306:19082145Test_seq1856846026H125M=185854321GATCAAAATTATTACTAATATAAAAAAATCAATAAGCTGGAAAATAAAGCAGAAGACACTTTTGAAAAATTAGAGCAAAATGACATTGTTGGAAAATATGAGGGAAAGAATAGCTAAATACTACTFFFFFFFFF,FFFFFFFFFFFF:FFFFF,FFFFF:FFF,,FFFFFFFFFFFFFF,F,FFF::FFFFFF,F:F:FF,F:FFFFFFFF,F,F,FFFFF,F:,FF,FF:F,:FFF:,:F:F::,FF,,NM:i:3MD:Z:68T17G1G36MC:Z:151MAS:i:110XS:i:0SA:Z:Test_seq,255993,-,125S26M,42,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:11306:1908113Test_seq25599342125S26M=185854-70015AGTAGTATTTAGCTATTCTTTCCCTCATATTTTCCAACAATGTCATTTTGCTCTAATTTTTCAAAAGTGTCTTCTGCTTTATTTTCCAGCTTATTGATTTTTTTATATTAGTAATAATTTTGATCGATCTAAATGCCCAATCAACCAACTT,,FF,::F:F:,:FFF:,F:FF,FF,:F,FFFFF,F,F,FFFFFFFF:F,FF:F:F,FFFFFF::FFF,F,FFFFFFFFFFFFFF,,FFF:FFFFF,FFFFF:FFFFFFFFFFFF,FFFFFFFFF:FFFFF,F,F:FFFFFFFFFFFFFFFNM:i:0MD:Z:26MC:Z:151MAS:i:26XS:i:0SA:Z:Test_seq,185684,+,26S125M,60,3;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1102:7491:2785	!	0	Test_seq	168666	-	-	NR	A00126:224:H7HMWDSX3:3:1102:7491:278597Test_seq1682384324S127M=168516429CACTTGATGACCCAAAATCAGATTAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:2MD:Z:33T74G18MC:Z:151MAS:i:117XS:i:97XA:Z:Test_seq,-212674,127M24S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1102:7491:2785145Test_seq16851660151M=168238-429CAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAGTTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:79A71MC:Z:24S127MAS:i:146XS:i:82Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1102:22987:3724	Test_seq	272454	Test_seq	288164	-	+	UR	A00126:224:H7HMWDSX3:3:1102:22987:3724145Test_seq27243130127S24M=28816415711CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTTAAAATGTACAGCCATCATGAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,288412,-,128M23S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1102:22987:37242193Test_seq28841260128M23H=288164-376CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0SA:Z:Test_seq,272431,-,127S24M,30,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1102:22987:372497Test_seq28816460151M=272431-15711TAAGGCATGGTAGAACCAGAGATGTCCCACTACACTGCTTTCCACCTGGGGACACTGGGTACGGGAAAGGTTCCCTTAGAGTTCTAGCTTTTCTTTTTTTGAAGAATTTATACAACAGGGAATTTTGAGAAGCCAGCTAAAATGCCCACGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1102:22987:3724	Test_seq	272454	Test_seq	288164	-	+	UR	A00126:224:H7HMWDSX3:3:1102:22987:37242193Test_seq28841260128M23H=288164-376CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0SA:Z:Test_seq,272431,-,127S24M,30,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1102:22987:3724145Test_seq27243130127S24M=28816415711CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTTAAAATGTACAGCCATCATGAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,288412,-,128M23S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1102:22987:372497Test_seq28816460151M=272431-15711TAAGGCATGGTAGAACCAGAGATGTCCCACTACACTGCTTTCCACCTGGGGACACTGGGTACGGGAAAGGTTCCCTTAGAGTTCTAGCTTTTCTTTTTTTGAAGAATTTATACAACAGGGAATTTTGAGAAGCCAGCTAAAATGCCCACGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1102:11822:4335	Test_seq	166209	Test_seq	214876	-	-	UU	A00126:224:H7HMWDSX3:3:1102:11822:4335113Test_seq1661592651M=21482648668CAGGATAATGTAGGTCTCTGGTTCATGTCGCCCTTTCTGTCTCTTGGGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28G22MC:Z:51MAS:i:46XS:i:38XA:Z:Test_seq,+214826,51M,3;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:11822:4335177Test_seq2148262851M=166159-48668GAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTGFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFNM:i:0MD:Z:51MC:Z:51MAS:i:51XS:i:43XA:Z:Test_seq,+166159,51M,2;Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1102:31313:5556	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31313:555697Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31313:5556145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31313:55562193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RU
-A00126:224:H7HMWDSX3:3:1102:31394:5541	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31394:554197Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFF:F:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31394:5541145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFF,,FFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31394:55412193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:31313:5556	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31313:555697Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31313:55562193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31313:5556145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:31394:5541	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31394:554197Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFF:F:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31394:55412193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31394:5541145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFF,,FFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1102:4218:5822	Test_seq	170181	Test_seq	170586	+	-	UU	A00126:224:H7HMWDSX3:3:1102:4218:5822161Test_seq17018160151M=170436406TTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATGTTTTTTTACTTCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFF:FF:F:FFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFF,F,:FFFFFFFF::FFFFFFF,:FFFF:FF,FFF,FFFFFFF::,F::F:F:FFF:FFFFFFFFF,F:,FFFFF:FF:,NM:i:4MD:Z:15A78A7C1A46MC:Z:151MAS:i:131XS:i:55Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:4218:582281Test_seq17043660151M=170181-406TAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTFFFFFFFFFFFFF::F:FFFF,FFFFFFFFF:FFFFF:FFFFFF,FFFFFFFF:FF:FFFF,FFF:FFFFF:FFFFFFFFFF:FFFFF,F,FFFF,F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FF,FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:25Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:18602:6652	Test_seq	171219	Test_seq	171678	+	-	UU	A00126:224:H7HMWDSX3:3:1102:18602:6652161Test_seq17121960151M=171528460AAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:141G9MC:Z:151MAS:i:146XS:i:79Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:18602:665281Test_seq17152860151M=171219-460TGTTTCCCTGGTCCTCCAGAAGTCCCAGCCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGTGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCCTGCAACTTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:2A25T26T16C6T59T8A2MC:Z:151MAS:i:120XS:i:53Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:15619:9721	Test_seq	170074	Test_seq	170377	+	-	UU	A00126:224:H7HMWDSX3:3:1102:15619:9721161Test_seq17007425151M=170227304AAAATTTGTTTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAG,,:F::FFFFFFFFFF:FFFFFF,FFFFFFFF::FFFFFF::::::FFF:FF:F:FFFFFFFFFFFF:FFF:FF::FFFFFFFF:FFF:FFF,,FFFFFFF:FFF:FFFFFFFFF:FF:FFFFFFFFF:FFFF:FFFFFFFF:F,FFFFFFNM:i:7MD:Z:8A24C7G20A32A25C0A28MC:Z:151MAS:i:116XS:i:102XA:Z:Test_seq,-38508,147M4S,9;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:15619:972181Test_seq17022760151M=170074-304ATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGFF:FFFF::FF:FF,FFFF:FFF:F,F:FFFFF,FFF,FFF:F:FFFFFFFF:F,,FFF:FFF:FFFF:F:::FF,FFF::F,FFFFFF::F,FFFFFFFFFFFFFFF:F:FFFFFFFFFF:FFFFFFFFFFFF,FFFF:,:FFF:F,FFFNM:i:1MD:Z:35A115MC:Z:151MAS:i:146XS:i:78Yt:Z:UU
@@ -78,7 +78,7 @@
 A00126:224:H7HMWDSX3:3:1102:24379:34413	Test_seq	169290	Test_seq	169639	+	-	UU	A00126:224:H7HMWDSX3:3:1102:24379:3441397Test_seq16929060151M=169489350TATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:102Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:24379:34413145Test_seq16948960151M=169290-350ATTTGTTCAAGTTGTCAGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATAAAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:30C0C41T65T11MC:Z:151MAS:i:131XS:i:70Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:2031:34757	!	0	Test_seq	167704	-	-	NR	A00126:224:H7HMWDSX3:3:1102:2031:34757177Test_seq2135927127M24S=167554-46015CGATAACTGGTATCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGTGAAAGAATTTTCAGCTATGACATCTCAGACCAGCGCCTGATCGATCTCAAACTTTTGCCTAGTAGAF,:FFF,F,,,F,F::FFFFFFF,FFFFFF,F,FF:,F:F,F,F:F,FFF:FFFFFFFF:FF:FFFFF::,:F::FF:F,F,F,,:FFF:FF:FF:,F,F,:F,FFFF,FFF,FFFFFFFF,FFFF,FFF,F,FFF,FFFF,F:FF::F:FNM:i:6MD:Z:0A9G1G64T6G26C15MC:Z:151MAS:i:101XS:i:96XA:Z:Test_seq,+167316,24S127M,7;Test_seq,+400737,24S114M13S,5;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1102:2031:34757113Test_seq16755431151M=21359246015ATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCTCCAATTGACTGACACTGGTCCTTFFF:FFFFFF,:FF:FF:,FFFF:FFFFFF,:,FFF:FFFFFFFFF::F:,FFFFFFFF:F,F:F,FFFF,:FFF:F:FFF:FFFFF:FFF:FF,,FF:FF:,FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFF,FFF,FFFFF,FFNM:i:1MD:Z:127C23MC:Z:127M24SAS:i:146XS:i:131XA:Z:Test_seq,+213334,126M2I23M,4;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1102:12563:35650	Test_seq	346775	Test_seq	347269	+	-	UU	A00126:224:H7HMWDSX3:3:1102:12563:35650161Test_seq34677560151M=347119495GTTTCTTAGACATGGAAACCCTGGTGGTGTAGTGGTTAAGTGCTACGGGGCTGCTAACCAAGAGGTCGGCATTTCGAATCCACCAGGCACTCCTTGGAAACTCTATGGGGCAGTTCTACTCTGTCCTATAGGGTCGCTATGAGTCGGAATTFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFF:FFFF:F:FFFFFFFFF,FF:F,FF,FFF,FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:77Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:12563:3565081Test_seq34711960151M=346775-495ATATAAACCAAAAAATAGATAAGATAGAAAGTATTAGGCTAAGTTATGGAGAGATTTTTGAGGAGTTTTTTTTTTTTAATTTTTATTGTGCTTGAAGTGAAAGTTTACAAATCAAGTCAGTCTCTCATAGAAAAATTTATATACACCTTGCFFF:FFFFF:FFFF:F::FF::FFFF,FF::FF:FF,,FF:F:FFFF:FF:FFFFFFFFFF:FF::FFFFFF::FFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:59Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1103:19000:1892	!	0	Test_seq	169431	-	-	MR	A00126:224:H7HMWDSX3:3:1103:19000:1892177Test_seq4000600122S29M=169281-230658CAACTAAATAGACCCATAAAAAAAAACGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAGATCTTATAATAGTGGTCTTATACAGTAFFFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFF:FF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26A2MC:Z:151MAS:i:26XS:i:26SA:Z:Test_seq,168996,+,28S123M,60,2;XA:Z:Test_seq,-166639,122S29M,1;Test_seq,+214367,18M1I10M122S,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1103:19000:18922209Test_seq1689966028H123M=169281436TAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCGTTTTTTTTTATGGGTCTATTTAGTTGFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFF:FF:FFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFFFNM:i:2MD:Z:96T6G19MC:Z:151MAS:i:113XS:i:36SA:Z:Test_seq,400060,-,122S29M,0,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1103:19000:1892113Test_seq16928160151M=400060230658TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28T122MC:Z:122S29MAS:i:146XS:i:103Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1103:19000:1892	!	0	Test_seq	169431	-	-	MR	A00126:224:H7HMWDSX3:3:1103:19000:18922209Test_seq1689966028H123M=169281436TAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCGTTTTTTTTTATGGGTCTATTTAGTTGFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFF:FF:FFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFFFNM:i:2MD:Z:96T6G19MC:Z:151MAS:i:113XS:i:36SA:Z:Test_seq,400060,-,122S29M,0,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1103:19000:1892177Test_seq4000600122S29M=169281-230658CAACTAAATAGACCCATAAAAAAAAACGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAGATCTTATAATAGTGGTCTTATACAGTAFFFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFF:FF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26A2MC:Z:151MAS:i:26XS:i:26SA:Z:Test_seq,168996,+,28S123M,60,2;XA:Z:Test_seq,-166639,122S29M,1;Test_seq,+214367,18M1I10M122S,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1103:19000:1892113Test_seq16928160151M=400060230658TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28T122MC:Z:122S29MAS:i:146XS:i:103Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1103:30544:2378	!	0	Test_seq	168612	-	-	MU	A00126:224:H7HMWDSX3:3:1103:30544:2378161Test_seq1682780151M=168462335CTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGTTAGCTTTACTTCCTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCGTAATTGTFFFF:,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,:FFF:,FNM:i:12MD:Z:4T53C0A6C0G0G18A2A25T7C18C6C0MC:Z:151MAS:i:95XS:i:95XA:Z:Test_seq,-212610,151M,12;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1103:30544:237881Test_seq16846260151M=168278-335GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:91Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1103:25880:3098	Test_seq	183557	Test_seq	184047	+	-	UU	A00126:224:H7HMWDSX3:3:1103:25880:3098161Test_seq18355760151M=183897491ATCCAGTTGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCACFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:30Yt:Z:UU	A00126:224:H7HMWDSX3:3:1103:25880:309881Test_seq18389760151M=183557-491TCAATTCTCCGGTCTTTCTTAGTCATTGTTCAGCTTTCAAATGCATATTATGCGATTGAAAACACCATGGTTTGGGTCAGGTGCCCCTTAGTCCTCAAAGTGACATCTTTGCTTTTTAACACTTTAAAGAGGTCTTTTGCAGCAGATTTACFFFF:FFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:64Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1103:26639:3818	Test_seq	41455	Test_seq	214212	+	-	UU	A00126:224:H7HMWDSX3:3:1103:26639:381897Test_seq414556151M=214062172758TGGGCAAAGGATATGAACAGGCACTTCACTAAAGAAGACATTCAGGTAGCTAACAGATACATGAGGAAATGCTCATGATCATTAGCCATCAGAGAAATGCAAATCAAAACTATGACGAGATACCATTTCACCCCAACAAGGCTGGCATTAA:FFF:F:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F:FFFFFFFFFFFFFFFF:FF:FFFF:FFFFFF::FFFFFF:FFFFFFF,F:FNM:i:13MD:Z:20A25C0G26T0C13T22C0A1T5T4C4T15C3MC:Z:151MAS:i:87XS:i:81XA:Z:Test_seq,-49064,151M,14;Test_seq,-151371,151M,15;Test_seq,+258709,151M,16;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1103:26639:3818145Test_seq21406260151M=41455-172758AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCGTCF,FFFFFF:FFF,FFFFFFFFFF:FF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFF,FFFFF:FFFFF,FFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:148A2MC:Z:151MAS:i:148XS:i:116Yt:Z:UU
@@ -136,7 +136,7 @@
 A00126:224:H7HMWDSX3:3:1104:21658:7279	!	0	Test_seq	293960	-	-	NR	A00126:224:H7HMWDSX3:3:1104:21658:727997Test_seq2935986032S119M=293810363ACAAAAACATACTGTGAAAACGAGTCTAGAGTAATCCTAAACCTATACTTATGAATGTCATCCTGTCTGGAAATAGAGTTTTCTTTGTTATGTTAATGAGGTCATATCAGTGCCCTAAACCCAAGCTTTTGTGAGTTACAAGGAGAAGCATFFFFF::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FF,FFFFFFFFFFFFF,FFFF::FFFFFFFFFFF,FFFF::FFFFFFFFFFFFFFFFFFF:,FF,FFF:FFFFFFFF,FFFF:F,FFFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:21658:7279145Test_seq29381060151M=293598-363GAAAAAGACCAGAATCCTCCTCCACTGTCAAAAAGGAGCCTTCCCCTGGAGCAGGAGCCCTGAATTAGGACTTCCAGCCTCCAGACCAGTGAGAAAATAAATTTCTCTTCTTTAAAGCCCCCACTTGCAGTATTTCTGTTACAGCAGCACTFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFF:FFFFFFF,FFFFFF::FFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:85A65MC:Z:32S119MAS:i:146XS:i:0Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1104:8169:7498	!	0	Test_seq	167708	-	-	NR	A00126:224:H7HMWDSX3:3:1104:8169:749897Test_seq167315827S124M=167558394GCCCTCCTGTGGCCAGAAAAATCTCTCAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF::FFFFF:,FFFFFFFFFFF,FFFFFFFFFFFFFF,FNM:i:2MD:Z:8A44A70MC:Z:151MAS:i:114XS:i:109XA:Z:Test_seq,-213596,124M27S,3;Test_seq,+400736,27S124M,5;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:8169:7498145Test_seq16755831151M=167315-394TTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCCAATTGACTGACACTGGTCCTTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:118A32MC:Z:27S124MAS:i:146XS:i:131XA:Z:Test_seq,+213330,130M2I19M,4;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1104:5791:7921	!	0	Test_seq	171294	-	-	NR	A00126:224:H7HMWDSX3:3:1104:5791:7921161Test_seq1710696024S127M=171144226ACTATTCTAGCTATCAGCAAGGATTGATCTTTGGTTGGCAGTTTTTCTCGTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTFFFFFFFFFFFFFFFFFFFFFFF:FFFF::FFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:5C16A2C101MC:Z:151MAS:i:112XS:i:68Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:5791:792181Test_seq17114460151M=171069-226CTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGTTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:66C84MC:Z:24S127MAS:i:146XS:i:55Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1104:11568:9377	Test_seq	116069	Test_seq	168405	-	+	UR	A00126:224:H7HMWDSX3:3:1104:11568:9377145Test_seq11604721128S23M=16840552337CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCACAAAATGGAGGACAACTACAFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:19SA:Z:Test_seq,168651,-,126M25S,60,1;XA:Z:Test_seq,-85506,127S19M5S,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1104:11568:93772193Test_seq16865160126M25H=168405-372CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:65T60MC:Z:151MAS:i:121XS:i:55SA:Z:Test_seq,116047,-,128S23M,21,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1104:11568:937797Test_seq16840560151M=116047-52337TATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,:FF:FFFFFFFNM:i:1MD:Z:90T60MC:Z:128S23MAS:i:146XS:i:81Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1104:11568:9377	Test_seq	116069	Test_seq	168405	-	+	UR	A00126:224:H7HMWDSX3:3:1104:11568:93772193Test_seq16865160126M25H=168405-372CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:65T60MC:Z:151MAS:i:121XS:i:55SA:Z:Test_seq,116047,-,128S23M,21,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1104:11568:9377145Test_seq11604721128S23M=16840552337CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCACAAAATGGAGGACAACTACAFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:19SA:Z:Test_seq,168651,-,126M25S,60,1;XA:Z:Test_seq,-85506,127S19M5S,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1104:11568:937797Test_seq16840560151M=116047-52337TATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,:FF:FFFFFFFNM:i:1MD:Z:90T60MC:Z:128S23MAS:i:146XS:i:81Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1104:24921:11741	Test_seq	168602	Test_seq	212800	-	-	UU	A00126:224:H7HMWDSX3:3:1104:24921:11741177Test_seq16845260151M=21265044199TGTTGAATAAGAGCAGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATF:FFFFFFFFFF,FFFFFFF,,:,:,FFFFFFFFFFF,:FF:FFFFFFFFFFF:FF:FFFFFFFFF::FFFF:F:FFFFFFFFFFFFFFFFFF:FFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF::F:FFFF:FFFFNM:i:1MD:Z:14G136MC:Z:151MAS:i:146XS:i:93Yt:Z:UU	A00126:224:H7HMWDSX3:3:1104:24921:11741113Test_seq21265024151M=168452-44199GCAAGGAAGATTTAAAGTTATCACTTTTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCTGGTTATAAAATAAACATACAAAAATCACTTGGATTF,,,,F,,FF,,:FFF,F,FF,:::,FFF,:FF,FFFFFFF:FFFFF:FF,:F::,F,:FF,,:,:,:FF::FFF,:F:F,FF,:F:F,:::F,,:,FF,:FFF:FFF::,FFFFF,FFF:F,:FFFFF:FFF:F,FFF,FFF,FFF:FFFNM:i:11MD:Z:7G2A0G4A5T2A17C0T17T52A8G26MC:Z:151MAS:i:100XS:i:85XA:Z:Test_seq,+168238,151M,14;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1104:8323:13463	Test_seq	170316	Test_seq	170786	+	-	UU	A00126:224:H7HMWDSX3:3:1104:8323:13463161Test_seq17031660151M=170636471AAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAGTAAGTTTTTAGTGCTGTCATTTTGATGFFFFFFFFFFFFFFFFFF::FFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFF:FF:FFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFF,FF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF:FFFF:FFFFFFFF,FFFNM:i:1MD:Z:123A27MC:Z:151MAS:i:146XS:i:52Yt:Z:UU	A00126:224:H7HMWDSX3:3:1104:8323:1346381Test_seq17063660151M=170316-471CTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTTTGATCGTTTATCTATTGATTTCCATGATTFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:3A42G2G3A2A38G25G29MC:Z:151MAS:i:117XS:i:23Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1104:18855:14418	!	0	Test_seq	171064	-	+	NR	A00126:224:H7HMWDSX3:3:1104:18855:1441881Test_seq17129360129M22S=171064-358TTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTACAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTACTCATGGAGACCCCATGTTACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:81T25G15T5MC:Z:151MAS:i:114XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:18855:14418161Test_seq17106460151M=171293358ATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:1MD:Z:27A123MC:Z:129M22SAS:i:146XS:i:73Yt:Z:NR
@@ -182,7 +182,7 @@
 A00126:224:H7HMWDSX3:3:1105:23077:15562	!	0	Test_seq	167509	-	-	NR	A00126:224:H7HMWDSX3:3:1105:23077:15562161Test_seq1671312225S126M=167359379CCTCCACCCTCTTAAAAATATGTTAAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:13C98T13MC:Z:151MAS:i:116XS:i:106XA:Z:Test_seq,-213778,126M25S,4;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:23077:1556281Test_seq16735917151M=167131-379CAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:9A141MC:Z:25S126MAS:i:146XS:i:138XA:Z:Test_seq,+213528,3S148M,2;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:27923:18443	!	0	Test_seq	168451	-	+	NR	A00126:224:H7HMWDSX3:3:1105:27923:1844381Test_seq16868960122M29S=168451-360CTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAATCTACAGTATGATAAAAAAAAAAGTTA:FFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:27T94MC:Z:151MAS:i:117XS:i:54Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:27923:18443161Test_seq16845160151M=168689360ATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA:FFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,:FFF:FFFF,FFFFFFFF:FFFFFF:FF,FFFNM:i:1MD:Z:44T106MC:Z:122M29SAS:i:146XS:i:93Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:8820:20337	Test_seq	171233	Test_seq	171572	+	-	UU	A00126:224:H7HMWDSX3:3:1105:8820:20337161Test_seq17123360151M=171441340TCTTTGGTTTTGGCAAGTTTGATGATAATATATCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFF:FFFFFFFFF,FFFFFFFFNM:i:1MD:Z:31G119MC:Z:132M19SAS:i:146XS:i:36Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:8820:2033781Test_seq17144160132M19S=171233-340CCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCGGCTATGTTGGTGTTGATTTCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTTGAGTTAATTTTTCTACTAFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:73A10A7C39MC:Z:151MAS:i:117XS:i:43Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1105:10276:21010	!	0	Test_seq	171288	-	-	MR	A00126:224:H7HMWDSX3:3:1105:10276:21010113Test_seq518770132S19M=171138119394AAAAGAAAGGTTTCCTTCAAGGGAGAATCAATAAGAATAAGTTCAGACTACTCAGCAGAAACCATGCAGGCAAGAAGGGAATGGGATGACGTATACAGAGCACTGAAGGAGAAAATCTGCCAACCAAGGATCGATCAAGTTGGTTGATTGTFFFFFF,,:,,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:19MC:Z:151MAS:i:19XS:i:0SA:Z:Test_seq,171070,+,19S132M,60,3;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1105:10276:210102145Test_seq1710706019H132M=171138219GATCCTTGGTTGGCAGATTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,,:,,FFFFFFNM:i:3MD:Z:16T4A23G86MC:Z:151MAS:i:117XS:i:74SA:Z:Test_seq,51877,-,132S19M,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1105:10276:21010177Test_seq17113860151M=51877-119394TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTGGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:105T45MC:Z:132S19MAS:i:146XS:i:0Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1105:10276:21010	!	0	Test_seq	171288	-	-	MR	A00126:224:H7HMWDSX3:3:1105:10276:210102145Test_seq1710706019H132M=171138219GATCCTTGGTTGGCAGATTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,,:,,FFFFFFNM:i:3MD:Z:16T4A23G86MC:Z:151MAS:i:117XS:i:74SA:Z:Test_seq,51877,-,132S19M,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1105:10276:21010113Test_seq518770132S19M=171138119394AAAAGAAAGGTTTCCTTCAAGGGAGAATCAATAAGAATAAGTTCAGACTACTCAGCAGAAACCATGCAGGCAAGAAGGGAATGGGATGACGTATACAGAGCACTGAAGGAGAAAATCTGCCAACCAAGGATCGATCAAGTTGGTTGATTGTFFFFFF,,:,,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:19MC:Z:151MAS:i:19XS:i:0SA:Z:Test_seq,171070,+,19S132M,60,3;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1105:10276:21010177Test_seq17113860151M=51877-119394TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTGGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:105T45MC:Z:132S19MAS:i:146XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1105:18837:21089	Test_seq	165754	Test_seq	166211	+	-	UU	A00126:224:H7HMWDSX3:3:1105:18837:2108997Test_seq16575460151M=166061458AGTCAGCCCTTTCAGTCTCTCGTTTCGTGCCAATTTTGCCGTCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTAATTTAATTAGCTCACTCTTCATCAGCATCTCTCTCCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:3MD:Z:40A5T75G28MC:Z:151MAS:i:136XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:18837:21089145Test_seq16606160151M=165754-458TATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAATTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:82C68MC:Z:151MAS:i:146XS:i:75Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1105:16640:21167	!	0	Test_seq	167661	-	-	NR	A00126:224:H7HMWDSX3:3:1105:16640:2116797Test_seq167316722S129M=167511346TGAACCCTGATTGATTTGGATCGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFF:FF:FFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFF,FFFFFFFF:FFFFFFFFF:FFFFFNM:i:4MD:Z:7A37G6A73T2MC:Z:151MAS:i:111XS:i:106XA:Z:Test_seq,-213590,129M22S,5;Test_seq,+400737,22S123M6S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:16640:21167145Test_seq16751160151M=167316-346TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:22S129MAS:i:151XS:i:122XA:Z:Test_seq,+213377,83M2I23M2I37M4S,5;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:7835:24173	Test_seq	171202	Test_seq	171530	+	-	UU	A00126:224:H7HMWDSX3:3:1105:7835:2417397Test_seq17120260151M=171381329CTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGCGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:108A42MC:Z:101M1I49MAS:i:146XS:i:76Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:7835:24173145Test_seq17138160101M1I49M=171202-329TTTTCTCTGTGTTTTCTGTCCCCTCTTCCTGTTCTGGGACTCCAATCACTCACAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTCCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:19G3C2C8T15G46T45A5MC:Z:151MAS:i:108XS:i:28Yt:Z:UU
@@ -215,7 +215,7 @@
 A00126:224:H7HMWDSX3:3:1106:18222:16736	Test_seq	168314	Test_seq	168630	+	-	UU	A00126:224:H7HMWDSX3:3:1106:18222:1673697Test_seq16831411151M=168480317TGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGATAACTTTACTTCTTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCCTGATTGCCCTGGCTAAGACTTCCAACACGATGTTGAATAAGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F:F:FFFFFNM:i:15MD:Z:22C0A6C0G0G18A5G9C12T7C20A13G6A1G0T17MC:Z:151MAS:i:76XS:i:68XA:Z:Test_seq,-212574,151M,17;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:18222:16736145Test_seq16848060151M=168314-317TCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGG:FFFFFFFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:15T135MC:Z:151MAS:i:146XS:i:91Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:18747:17018	Test_seq	170380	Test_seq	170837	+	-	UU	A00126:224:H7HMWDSX3:3:1106:18747:17018161Test_seq17038060151M=170691458ACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFNM:i:1MD:Z:44C106MC:Z:73M4I74MAS:i:146XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:18747:1701881Test_seq1706916073M4I74M=170380-458CACCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGATCATTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:40G106MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:16251:18740	Test_seq	225248	Test_seq	308334	+	+	UR	A00126:224:H7HMWDSX3:3:1106:16251:18740129Test_seq2252486029M122S=30833483087GTTCTGATGTTGTTGTCATTTACAGATTATAAGTCAATCTCTTTTGAGATATAAAAGAGAGAAGTGAGGAGAAAGACATGGAGAACTCATGCTACCAAGAAACAAGATCCAGGAGAATAGCATGTCCTTTGGACCCAGGGTCCCCGTGGCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FF,FFFFFFFFFFFFF:FFNM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,308589,-,122M29S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1106:16251:187402193Test_seq30858960122M29H=308334-377AGCCACGGGGACCCTGGGTCCAAAGGACATGCTATTCTCCTGGATCTTGTTTCTTGGTAGCATGAGTTCTCCATGTCTTTCTCCTCACTTCTCTCTTTTATATCTCAAAAGAGATTGACTTAFF:FFFFFFFFFFFFF,FF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:24SA:Z:Test_seq,225248,+,29M122S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1106:16251:1874065Test_seq30833460151M=225248-83087TCCATAACATACCACTGTAGTGCCCCACATAGAATCCATTTTTATAATATGCTTAGCTTAGTTGTCTTAGTTATCCAGTGCTGCTATAACAGAAATAACACAAGTGGATGGCTTTAACAAACAGAAAGTTATTCTCTACAGTCTAGGAGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:29M122SAS:i:151XS:i:35Yt:Z:UR
-A00126:224:H7HMWDSX3:3:1106:31901:18756	Test_seq	56593	Test_seq	115194	+	+	RU	A00126:224:H7HMWDSX3:3:1106:31901:18756129Test_seq5659360151M=11519458602CTTCCGTATGGATATTATCCTATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCCAAAATGGCCAFFFFFF,,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFFFFF:FFFFFFFFFFF:,FFFFFFFFFFFFF:,FFFFFFF:NM:i:0MD:Z:151MC:Z:5S23M123SAS:i:151XS:i:103Yt:Z:RU	A00126:224:H7HMWDSX3:3:1106:31901:1875665Test_seq115194245S23M123S=56593-58602TGCTCTGTCACATGGGGTTGCTATGAGTAGTCAAGCTTTTGGGACCTTCATTTACTGATATGGCATAACTCAAAATGAGAAGAAATAGCTGCAAACATTCAGTAATAATCAGAATATGGAATGTACAAAATATAAATCTAGGAAAGTTGGAFFFFFFFFF:FF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,56816,-,123M28S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1106:31901:187562129Test_seq5681660123M28H=56593-346TCCAACTTTCCTAGATTTATATTTTGTACATTCCATATTCTGATTATTACTGAATGTTTGCAGCTATTTCTTCTCATTTTGAGTTATGCCATATCAGTAAATGAAGGTCCCAAAAGCTTGACTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:FFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0SA:Z:Test_seq,115194,+,5S23M123S,24,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1106:31901:18756	Test_seq	56593	Test_seq	115194	+	+	RU	A00126:224:H7HMWDSX3:3:1106:31901:18756129Test_seq5659360151M=11519458602CTTCCGTATGGATATTATCCTATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCCAAAATGGCCAFFFFFF,,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFFFFF:FFFFFFFFFFF:,FFFFFFFFFFFFF:,FFFFFFF:NM:i:0MD:Z:151MC:Z:5S23M123SAS:i:151XS:i:103Yt:Z:RU	A00126:224:H7HMWDSX3:3:1106:31901:187562129Test_seq5681660123M28H=56593-346TCCAACTTTCCTAGATTTATATTTTGTACATTCCATATTCTGATTATTACTGAATGTTTGCAGCTATTTCTTCTCATTTTGAGTTATGCCATATCAGTAAATGAAGGTCCCAAAAGCTTGACTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:FFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0SA:Z:Test_seq,115194,+,5S23M123S,24,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1106:31901:1875665Test_seq115194245S23M123S=56593-58602TGCTCTGTCACATGGGGTTGCTATGAGTAGTCAAGCTTTTGGGACCTTCATTTACTGATATGGCATAACTCAAAATGAGAAGAAATAGCTGCAAACATTCAGTAATAATCAGAATATGGAATGTACAAAATATAAATCTAGGAAAGTTGGAFFFFFFFFF:FF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,56816,-,123M28S,60,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1106:24795:22357	Test_seq	169327	Test_seq	169680	+	-	UU	A00126:224:H7HMWDSX3:3:1106:24795:22357161Test_seq16932760151M=169530354GCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFF,FFFFF:FFFF:FF:FFFF:FFFFFFFFFFFFFFNM:i:1MD:Z:140G10MC:Z:151MAS:i:146XS:i:111Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:24795:2235781Test_seq16953057151M=169327-354TTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCAGCCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:27C11G58T5T0T0T3A22G17MC:Z:151MAS:i:111XS:i:78Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:9127:23187	Test_seq	168739	Test_seq	169233	+	-	UU	A00126:224:H7HMWDSX3:3:1106:9127:23187161Test_seq16873960151M=169083495AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:81Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:9127:2318781Test_seq16908360151M=168739-495TTCAATCTGTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACGTAGTAATCTGAAGTGATTFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:8C62G2T3G7C31C26A5MC:Z:151MAS:i:116XS:i:65Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:17309:23328	Test_seq	168424	Test_seq	168810	+	-	UU	A00126:224:H7HMWDSX3:3:1106:17309:23328161Test_seq16842460151M=168660387ATTGCCCTGGCTACGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCCGTTGGCTTTGCATAGATGCCCTTTATTAF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:13G57T50T28MC:Z:151MAS:i:136XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:17309:2332881Test_seq16866060151M=168424-387ATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:56T94MC:Z:151MAS:i:146XS:i:55Yt:Z:UU
@@ -223,7 +223,7 @@
 A00126:224:H7HMWDSX3:3:1106:27308:24862	Test_seq	167312	Test_seq	214100	-	-	UU	A00126:224:H7HMWDSX3:3:1106:27308:24862177Test_seq16716252151M=21395046789TAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:81T69MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213722,151M,6;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:27308:24862113Test_seq21395037151M=167162-46789AATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTTATACGCTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTTTCTTAAACAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGF:F:::FFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:28C14A49T11A45MC:Z:151MAS:i:131XS:i:112XA:Z:Test_seq,+166938,4S147M,7;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:2284:29434	!	0	Test_seq	167543	-	-	NR	A00126:224:H7HMWDSX3:3:1106:2284:2943497Test_seq1671734428S123M=167393371GTGAAGATGCATCTACATGCCAAGGATCGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGAC:FFFFF:::FF:F:FFFF::F:,F:,F:FFFFFF:F,:FFFFF:FFFFFFFF:FFFFFFFFF,:FF,FFFF:FF,FFFF,FF,F:F:F:,FFF:FFFF,:FFF:F::F,F,:F,F,FFF:FFFFFFFFF,FFFF:,F::FFF,FFFFFFF,NM:i:2MD:Z:5A64T52MC:Z:151MAS:i:113XS:i:93XA:Z:Test_seq,-213739,123M28S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1106:2284:29434145Test_seq16739360151M=167173-371AGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATCTTTATCGTFF:FFFFFFFFF:,,FFFF:,FFFFFFFFFFFFF:FFFFFF,F,FFFF,FFFF:FF:FFF:FF:FF:F:FF,FFFFFFFFF:FFFFFF,:FFFFF,,FFFFFFFF,:FFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:118T32MC:Z:28S123MAS:i:146XS:i:117XA:Z:Test_seq,+213491,29M4D4M4I114M,10;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1106:3125:29763	Test_seq	169989	Test_seq	170460	+	-	UU	A00126:224:H7HMWDSX3:3:1106:3125:29763161Test_seq16998948151M=170310472GGATGTCCTGTCCTTCTCCGAAAGTGGTGTGTTAAAGTCTCCTACTATAATTGTGGAGGTATCTATATCACTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATTTCT,FFFF:FFF,FF,:FF:F,,FFFFF::F,FF,:F:FF:FFF,FFFFFFF,FFFF,FFFF:FFFFFFFFFFF:FFF:FFFFFF,F:F:F:FFFFFFFFFF,FFF,FF,F,F,,:F,F:FFFF,,FFFFFFFFFFFFFFFFFFF,FFFF,::FNM:i:10MD:Z:33G5C10A7A1G5C2G48C7G20A3MC:Z:151MAS:i:102XS:i:73Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:3125:2976381Test_seq17031060151M=169989-472GTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:66C84MC:Z:151MAS:i:146XS:i:63Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1106:29026:29841	!	0	Test_seq	166159	-	+	MR	A00126:224:H7HMWDSX3:3:1106:29026:2984165Test_seq38347205S21M125S=166159-217314GCCTTGAGTTGCAATAGTGAAGGATTGATCAATCAGGAGATAAGAACTGCTTTGGATGAAGGGAAAGACAACACACAATACAAGGAAGGTCAGCCTAATTGGACTGGACTAAAAGCAAAGAGGTTTCCGGGATAAAATGAAAGCTTCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:21SA:Z:Test_seq,166429,-,128M23S,60,2;XA:Z:Test_seq,-334091,125S26M,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:29026:298412129Test_seq16642960128M23H=166159-398CTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCATCCAAAGCAGTTCTTATCTCCTGATTGATCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:95C18A13MC:Z:151MAS:i:118XS:i:28SA:Z:Test_seq,383472,+,5S21M125S,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1106:29026:29841129Test_seq16615960151M=383472217314CAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGGTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:::FFFFFFNM:i:1MD:Z:102T48MC:Z:5S21M125SAS:i:146XS:i:77Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1106:29026:29841	!	0	Test_seq	166159	-	+	MR	A00126:224:H7HMWDSX3:3:1106:29026:298412129Test_seq16642960128M23H=166159-398CTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCATCCAAAGCAGTTCTTATCTCCTGATTGATCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:95C18A13MC:Z:151MAS:i:118XS:i:28SA:Z:Test_seq,383472,+,5S21M125S,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:29026:2984165Test_seq38347205S21M125S=166159-217314GCCTTGAGTTGCAATAGTGAAGGATTGATCAATCAGGAGATAAGAACTGCTTTGGATGAAGGGAAAGACAACACACAATACAAGGAAGGTCAGCCTAATTGGACTGGACTAAAAGCAAAGAGGTTTCCGGGATAAAATGAAAGCTTCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:21SA:Z:Test_seq,166429,-,128M23S,60,2;XA:Z:Test_seq,-334091,125S26M,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1106:29026:29841129Test_seq16615960151M=383472217314CAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGGTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:::FFFFFFNM:i:1MD:Z:102T48MC:Z:5S21M125SAS:i:146XS:i:77Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1106:15591:30123	Test_seq	171134	Test_seq	171455	+	-	UU	A00126:224:H7HMWDSX3:3:1106:15591:3012397Test_seq17113460151M=171323322TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTG:FF:FFFFFF:,FF:F:F,,FF:F:F,F::,FFFFF,FFFF,:::::F:FFFFFFF,,FF::F:F:FFFFFFF:FFFFFF,,F,FFF,:FFFF:F,,:F,F,,,FF:,::FFF,,FF,FF,,FF:F,,,FF,FF,FFFF,F,FFFF,F,:FNM:i:1MD:Z:118T32MC:Z:133M18SAS:i:146XS:i:63Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:15591:30123145Test_seq17132360133M18S=171134-322GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAG::::,FFF,:F:FFF,FFF:FF,:FF:FF:FF:FF,F:,F:F:::FF::F,FFFFF,,,,,FF:F::::F,,FF,F,FFFFFF:FFF,F:FF:F::FF,FF,FFF,F,FFFFFF,F,:FF,FFFF:F,FFFFFF:FF::FFFFFF:FF:,FNM:i:3MD:Z:46G30G15T39MC:Z:151MAS:i:118XS:i:38Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:30138:30483	Test_seq	183328	Test_seq	183714	+	-	UU	A00126:224:H7HMWDSX3:3:1106:30138:30483161Test_seq1833286026M4I121M=183564387TGTTCTGCATCTATTAAAAATGATGAGATCGATCCATGTAGCTGTAGCAGTGGTGGGTTAGAGTTTTAGGTCTGTAGCCAGTAGATGTATGTTTGTATTTTCTCTCTGCCACTTACTAGTTGTGTTAATTTGAGCAAGTCATTTGACCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFF::FFFFFFNM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:30138:3048381Test_seq18356460151M=183328-387TGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCACTGACCCTFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:,FFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFF:FFFFF:FFFFFFF:FFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:26M4I121MAS:i:151XS:i:52Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:15890:30639	Test_seq	171134	Test_seq	171455	+	-	UU	A00126:224:H7HMWDSX3:3:1106:15890:3063997Test_seq17113460151M=171323322TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,::FFFFFFFFFFFF,:FF:F:FFFF,FFFFFF:FF:FFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:118T32MC:Z:133M18SAS:i:146XS:i:63Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:15890:30639145Test_seq17132360133M18S=171134-322GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FF,:FFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:46G30G15T39MC:Z:151MAS:i:118XS:i:38Yt:Z:UU
@@ -232,7 +232,7 @@
 A00126:224:H7HMWDSX3:3:1106:14362:33599	Test_seq	167148	Test_seq	167571	+	-	UU	A00126:224:H7HMWDSX3:3:1106:14362:3359997Test_seq16714841151M=167421424AATTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF::FF,FFFF:FFF:FFFFFF,F,FFFFFFF,F:FFFF,:FFFNM:i:2MD:Z:30A64T55MC:Z:151MAS:i:141XS:i:121XA:Z:Test_seq,-213736,151M,6;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:14362:33599145Test_seq16742160151M=167148-424TTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTGGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFF:FF:,FFFFFFFFFFFF:FFFFF:F,FFFFFFNM:i:1MD:Z:85A65MC:Z:151MAS:i:146XS:i:106Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:27236:35697	Test_seq	406947	Test_seq	407376	+	-	UU	A00126:224:H7HMWDSX3:3:1106:27236:35697161Test_seq4069476061M4I86M=407226430TCCAGGGACCTCAGCTATTCCATGAGATAATTTTACAAATGGTAGCCAGTGTCCTGTTTCAGATCGATCACAAATGTCCCTGTGGCCCCACTAGTGTCTTAAACATTATATAGGAACAAGGCTTTGCAGGGCAAAGACAGCAGTCCACTAGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:F:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:27236:3569781Test_seq40722660151M=406947-430GTGCAGCTTGTCCTTGCTCTTCAACTTTGTGTTGAGAAGTAAATAATAAGCTTGAATGCTCTTTTGCAGATAATCCTAACAGGGGAACCAACACTATAGCTAGAGATTCAGGAGAATTTGAAAAGAGGTTTACTCATTTGGAAATGGGAGAF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:61M4I86MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:29008:36886	Test_seq	167972	Test_seq	168330	+	-	UU	A00126:224:H7HMWDSX3:3:1106:29008:36886161Test_seq16797237151M=168180359TCGGAAGTGCGTTAAATGTATAGATGGCATTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGTAGAGCTTTGTAGTTTTCTTTCTATAGFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFF:FF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:10MD:Z:1T12T13T50G8C35C4T3A11G1G3MC:Z:151MAS:i:108XS:i:86Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:29008:3688681Test_seq16818042151M=167972-359TGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F:FFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:91T59MC:Z:151MAS:i:146XS:i:126XA:Z:Test_seq,+212708,151M,5;Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1107:20654:1313	Test_seq	382625	Test_seq	435035	-	-	UR	A00126:224:H7HMWDSX3:3:1107:20654:1313177Test_seq38260035125S26M=43488552411TGGAATTCTAGGTCTTCAAAGTGTTTGCTCTCAACATCCAACAGGTCAGCATCCTTTGGGGAAAAGGGAATGAAAACAAGACACACCAGAATGGCTGTAGCCTTAACATGAAGAAGCAATTATTATAATATGACTATTATTCAAATTTACTFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A0MC:Z:151MAS:i:25XS:i:0SA:Z:Test_seq,434625,+,26S125M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1107:20654:13132209Test_seq4346256026H125M=434885411TAATAATTGCTTCTTCATGTTAAGGCTACAGCCATTCTGGTGTGTCTTGTTTTCATTCCCTTTTCCCCAAAGGATGCTGACCTGTTGGATGTTGAGAGCAAACACTTTGAAGACCTAGAATTCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:125MC:Z:151MAS:i:125XS:i:0SA:Z:Test_seq,382600,-,125S26M,35,1;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1107:20654:1313113Test_seq43488560151M=382600-52411AAAAAAAAAAAAATTAAAATACACTCCTTGTATGTGACAGTAAAGATGGTCAGTATAGCTTTAACGTGTTATCTGTATGTCTGCATGTCTTTGTACCTGAACACCTTTGTGTATTTAATCACATATGGTATTTGGCAGACTGTGGATTCTGFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:125S26MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1107:20654:1313	Test_seq	382625	Test_seq	435035	-	-	UR	A00126:224:H7HMWDSX3:3:1107:20654:13132209Test_seq4346256026H125M=434885411TAATAATTGCTTCTTCATGTTAAGGCTACAGCCATTCTGGTGTGTCTTGTTTTCATTCCCTTTTCCCCAAAGGATGCTGACCTGTTGGATGTTGAGAGCAAACACTTTGAAGACCTAGAATTCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:125MC:Z:151MAS:i:125XS:i:0SA:Z:Test_seq,382600,-,125S26M,35,1;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1107:20654:1313177Test_seq38260035125S26M=43488552411TGGAATTCTAGGTCTTCAAAGTGTTTGCTCTCAACATCCAACAGGTCAGCATCCTTTGGGGAAAAGGGAATGAAAACAAGACACACCAGAATGGCTGTAGCCTTAACATGAAGAAGCAATTATTATAATATGACTATTATTCAAATTTACTFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A0MC:Z:151MAS:i:25XS:i:0SA:Z:Test_seq,434625,+,26S125M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1107:20654:1313113Test_seq43488560151M=382600-52411AAAAAAAAAAAAATTAAAATACACTCCTTGTATGTGACAGTAAAGATGGTCAGTATAGCTTTAACGTGTTATCTGTATGTCTGCATGTCTTTGTACCTGAACACCTTTGTGTATTTAATCACATATGGTATTTGGCAGACTGTGGATTCTGFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:125S26MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1107:4788:1892	Test_seq	166087	Test_seq	166508	+	-	UU	A00126:224:H7HMWDSX3:3:1107:4788:189297Test_seq16608760151M=166358422GGAGTTGTCTGTTGTGTTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTFFFFFFFFF:F:FFFF:FFF:,FFFF:FFF,FFFFFFFFFFFFFFFFFFFF,FF,FFFF::FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFF:FFFF,FFFFFFFFFFFF,F:FFFFFFFFNM:i:1MD:Z:16C134MC:Z:151MAS:i:146XS:i:74Yt:Z:UU	A00126:224:H7HMWDSX3:3:1107:4788:1892145Test_seq16635860151M=166087-422ATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTG,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:43Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1107:7120:2801	Test_seq	165764	Test_seq	166210	+	-	UU	A00126:224:H7HMWDSX3:3:1107:7120:280197Test_seq1657646016S135M=166060447ACTTGCTCTCCCCCTATTCAGTCTCTTGTTTCGTGCCAATTTTGCCATCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTGATTTAATTAGCTCACTCTTCATCAGCATCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:10C25T66A8G22MC:Z:151MAS:i:115XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1107:7120:2801145Test_seq16606060151M=165764-447GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAAGGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:107T43MC:Z:16S135MAS:i:146XS:i:75Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1107:29279:4225	Test_seq	167906	Test_seq	168348	+	-	UU	A00126:224:H7HMWDSX3:3:1107:29279:422597Test_seq16790660151M=168198443TTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:41Yt:Z:UU	A00126:224:H7HMWDSX3:3:1107:29279:4225145Test_seq16819839151M=167906-443GGTTATTTCCTCTTCGATGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTTTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATC:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:4MD:Z:16G56T18C55G2MC:Z:151MAS:i:133XS:i:113XA:Z:Test_seq,+212690,151M,8;Yt:Z:UU
@@ -274,7 +274,7 @@
 A00126:224:H7HMWDSX3:3:1108:9634:5588	Test_seq	40166	Test_seq	212883	+	-	UU	A00126:224:H7HMWDSX3:3:1108:9634:5588161Test_seq40166179M3I69M=212733172718CAACAGCCAACATCACCCTAAATGGGGAGAGACTGAAAGCATTCCCCTTGAGAACGGGAACCAGACAAGGATGCCTTTTATCACCACTCGTATTCAACACTGTGCTAGAGGTCCTAGCCAGAGCAATTAGGCTAGGCAAAGAAATAAAGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFNM:i:16MD:Z:15T9A5C11T21G9C5T0G3T9T6G28A0T14MC:Z:151MAS:i:74XS:i:71XA:Z:Test_seq,+212488,151M,16;Test_seq,-168400,151M,17;Test_seq,-50359,64M13I74M,24;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:9634:558881Test_seq21273360151M=40166-172718CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:104A46MC:Z:79M3I69MAS:i:146XS:i:116Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:16071:6872	Test_seq	36103	Test_seq	167548	+	+	UR	A00126:224:H7HMWDSX3:3:1108:16071:6872129Test_seq361031026M125S=167548131446TGCAAAGGCAATTCTGATAGGGATCTAAGGGAATAGAGAAACAAATCACTAATTTCATATGGAAAGGAAAGAACCCCCGGATAAGCAAAACATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTCFFFF:FFFF:::FFFFFFFFFF::F,,FFFFF,::F:FF::FF,FFFFFF,FFFFFF,FFFF,,FFFF,FFF::FFFFFF:F,,FFF::FF:F::FFFF,F:FFF:F,:FFF,F:FFFFFFFFF:FF,F,,F:FF,:,FFFFF,FFF,F:,NM:i:1MD:Z:20T5MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,167824,-,126M25S,60,4;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1108:16071:68722193Test_seq16782460126M25H=167548-402GAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGTTTTGCTTATCCGGGGGTTCTTTCCTTTCCATATGAAATTAGTGATTTGTTTCTCTATTCCCTTA,:F,FFF,FFFFF,:,FF:F,,F,FF:FFFFFFFFF:F,FFF:,F:FFF:F,FFFF::F:FF::FFF,,F:FFFFFF::FFF,FFFF,,FFFF,FFFFFF,FFFFFF,FF::FF:F::,FFFFF,,NM:i:4MD:Z:0T60C24C32C6MC:Z:151MAS:i:110XS:i:75SA:Z:Test_seq,36103,+,26M125S,10,1;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1108:16071:687265Test_seq16754842151M=36103-131446GTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACTGF:F,FFFFFFF::FFFFFFFF:F:,:FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF,FFF:F::FFFFF:FFF:FFFFF::,FFFF,F:F:::FFF,FF,::F:F:F,:FFFFFF:F:FF::F,FF:FFF:::,,,FFF::FFF:::F:NM:i:1MD:Z:127C23MC:Z:26M125SAS:i:146XS:i:126XA:Z:Test_seq,-213340,120M2I24M5S,4;Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1108:7292:7576	Test_seq	167298	Test_seq	167624	+	-	UU	A00126:224:H7HMWDSX3:3:1108:7292:7576161Test_seq1672982119S132M=167474327ATGAGGGTAATGATGATTAAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFNM:i:2MD:Z:25A44A61MC:Z:151MAS:i:122XS:i:112XA:Z:Test_seq,-213605,132M19S,4;Test_seq,+400719,19S132M,6;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:7292:757681Test_seq16747460151M=167298-327GTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:19S132MAS:i:151XS:i:104Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1108:24126:7701	Test_seq	55744	Test_seq	65171	+	-	RU	A00126:224:H7HMWDSX3:3:1108:24126:770197Test_seq5574460151M=651449428TCTCAACAGAGAAGCTGCCAAAACACTGAAAATCTTGGCCTGGCCAATTTGACACACAATCTTAACCATCACAAATAGTTCGTCTTCTTGAGCAGTCCTTTGAAATCTTCTGTTCAGCTCTTTTACTTAATCATTTCTTCCTTTCAATTTAFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:151MC:Z:123S28MAS:i:151XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:24126:7701145Test_seq6514454123S28M=55744-9428TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTATAAGAACCAGAGGTCAGATGATGACGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:28MC:Z:151MAS:i:28XS:i:0SA:Z:Test_seq,56015,-,123M28S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:24126:77012193Test_seq5601560123M28H=55744-394TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:23SA:Z:Test_seq,65144,-,123S28M,54,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1108:24126:7701	Test_seq	55744	Test_seq	65171	+	-	RU	A00126:224:H7HMWDSX3:3:1108:24126:770197Test_seq5574460151M=651449428TCTCAACAGAGAAGCTGCCAAAACACTGAAAATCTTGGCCTGGCCAATTTGACACACAATCTTAACCATCACAAATAGTTCGTCTTCTTGAGCAGTCCTTTGAAATCTTCTGTTCAGCTCTTTTACTTAATCATTTCTTCCTTTCAATTTAFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:151MC:Z:123S28MAS:i:151XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:24126:77012193Test_seq5601560123M28H=55744-394TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:23SA:Z:Test_seq,65144,-,123S28M,54,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:24126:7701145Test_seq6514454123S28M=55744-9428TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTATAAGAACCAGAGGTCAGATGATGACGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:28MC:Z:151MAS:i:28XS:i:0SA:Z:Test_seq,56015,-,123M28S,60,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1108:21061:8187	Test_seq	168347	Test_seq	168776	+	-	UU	A00126:224:H7HMWDSX3:3:1108:21061:8187161Test_seq1683476017S134M=168626430AAATGTTTGAACTTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::FFFFFFFFFNM:i:2MD:Z:18A21G93MC:Z:151MAS:i:124XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:21061:818781Test_seq16862660151M=168347-430ATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:90T60MC:Z:17S134MAS:i:146XS:i:72Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:18909:8469	Test_seq	104742	Test_seq	114432	+	-	UR	A00126:224:H7HMWDSX3:3:1108:18909:846997Test_seq1047426031M120S=1142829691CACATATCAGGTGTCAACAGGGAAGTGATCAATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTTFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:31MC:Z:151MAS:i:31XS:i:0SA:Z:Test_seq,114039,+,30S121M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1108:18909:84692145Test_seq1140396030H121M=114282394AATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:73SA:Z:Test_seq,104742,+,31M120S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1108:18909:8469145Test_seq11428260151M=104742-9691CAGCACAACTGGGCCAAAGCAAAACAAAGAAATTTCCTGGGCACATCTAAACACTGCAAGGGACAGAGTAGCTGGGGTTGGCGATTGGGGACCATAGTTTTGGGGAACATCTAGGTCAACTGCAATAATGAAGTTTATTAATAAAATGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:81T69MC:Z:31M120SAS:i:146XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1108:14498:8656	Test_seq	171559	Test_seq	171939	+	-	UU	A00126:224:H7HMWDSX3:3:1108:14498:865697Test_seq17155960151M=171789381ACATTCTAATTGCTCAAGTCTGCTCCTCTGACTTTCTATTGCATTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGATGTCTCTCTACGGATTCTTGCAACTTATTAATTTTTCCACTATGTTCTTGAATAATCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FF:FFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:F:FFFFFF:FFFFFF,FFFFFFFFFFFFF,FFFF:FFFNM:i:8MD:Z:15G8T17G5T41C10T30C13C4MC:Z:151MAS:i:111XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:14498:8656145Test_seq17178960151M=171559-381GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTGFFFFFFF:FFFFFF,FF:FFFFFF:FF::FFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFNM:i:1MD:Z:149C1MC:Z:151MAS:i:149XS:i:0Yt:Z:UU
@@ -291,7 +291,7 @@
 A00126:224:H7HMWDSX3:3:1108:30544:19789	!	0	Test_seq	167661	-	-	NR	A00126:224:H7HMWDSX3:3:1108:30544:1978997Test_seq167316722S129M=167511346CCGTGGGTATTGATTGTGGATCGATCAGGCGCTGGTCTGAGATATCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGTTGTTTGATTTTTAGGAGCTCCCAGTTATCTGG:FFF:F:FFFF,F,FF:::FFF,F,FFF,FFF::FFFFFFFFFFFFFFFFFFFFFF,FF,:F:F,FFFFFFF:F,:FFFF:FFFFFF:FFFFFFF,F:FF:,FF,F:F:FFFFFFFFF,FF,FFF:FF:FFF:FF,FFFFF:F,F:FFF,FNM:i:5MD:Z:7A13G23G6A43G32MC:Z:151MAS:i:104XS:i:99XA:Z:Test_seq,-213590,129M22S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:30544:19789145Test_seq16751160151M=167316-346TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTCAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGFFFF::FFFFFFFFF:F,F,FFFFFFFF,FFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFF::::FF:FFF:FFFFFF:FF:F:FFFFFFFFFFFFFF,FF::FFFF:FFFF:FFFFFFFFFFF::FFFFFFF::FFFFFFFFFFFF,FF:NM:i:1MD:Z:48T102MC:Z:22S129MAS:i:146XS:i:117XA:Z:Test_seq,+213377,83M2I23M2I37M4S,6;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1108:8187:20400	Test_seq	77443	Test_seq	233514	+	+	RU	A00126:224:H7HMWDSX3:3:1108:8187:2040065Test_seq7744360151M=233514156072TCCTCAGACAGTGCAATTTTCCTTGGCTTTTCAGCCCATAAAATTACTTGATAAACACTGTCTGCTTCTAAGCTTTCGGCCTTACACAGTTGCTTTGCTCCATTGTAAATAATTAAGAATTTAACAGGCCACTGATTAAAAACAAAATTGGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,,:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:::F:FFFFF:F,FFFF:F:FFFF:F:FFNM:i:1MD:Z:42G108MC:Z:27M124SAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:8187:20400129Test_seq2335144827M124S=77443-156072CTCAAACCCATTGCCGTCGAGTTGATTACTCCATATTGGCAAGTGCAGCAAGGCTGGGGCCCCTTCAGGACATATAGAATATAGCCAACATGAGGCCCAGGGTCAAGATCAGGGGGCCATTTGGTAGCCTCCAAAAGCAAAGACTAACATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFF::FFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFNM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,77734,-,124M27S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:8187:204002193Test_seq7773460124M27H=77443-415GATGTTAGTCTTTGCTTTTGGAGGCTACCAAATGGCCCCCTGATCTTGACCCTGGGCCTCATGTTGGCTATATTCTATATGTCCTGAAGGGGCCCCAGCCTTGCTGCACTTGCCAATATGGAGTFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFF::FFFFFF:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFNM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:0SA:Z:Test_seq,233514,+,27M124S,48,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1108:8495:20494	!	0	Test_seq	162287	-	+	NR	A00126:224:H7HMWDSX3:3:1108:8495:20494145Test_seq16242260126M25S=162287-261CTCAAAAACCCTATGGAAGAGTTCTTCTGTGCACACATGGGGTGGTCACGAGTCAGAATCGACTGGACTAGCAACTAACAACAGCAGGCAAGTTCGAAGGGTCCTCCTGGGTAGTTAGATGGGATCGATCTAATCTCTAAAATCTGTAGGAF:,F:F:FFFFFFFFFF,F:FFFFF:,FFFFFFF:F:FFFFFFFFFF,FF,:FF::,FFFF:FFFFFFFFFFFFFFFF:FFF:,FFF:F,FFFFFFFFFFFFF:FFFFFF,FFFFFFF:FFFFF:FFFF,,,FFFFFF:F::FFFFFFF:FNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:8495:2049497Test_seq16228760151M=162422261GGAGGAGGCAGGCAGAGGGAGGAGAAAAGCTTATTGGCTTATCATGGCATTAGTCATGTCAAGCTCACAGACACCCATTTCGAAAAGGAGAAAACAAGCTGGGGAGTATTAGGGTCACATTCTGAAAGGTAGAGCCTCAAAAACCCTATGGFFF:F:F,FFFFFFF:FFF:FFF:FFFFFF,FFFF:FF:FFF:FF::F:FFFF:FFFFFFFFFF:F,FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFFFF::FFFFF,FF:FFFFFFFFFFFFFFFFFFF:,FFFFFFFFFF:FFFFFFNM:i:1MD:Z:66C84MC:Z:126M25SAS:i:146XS:i:0Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1108:7744:20509	Test_seq	169333	Test_seq	170167	+	-	RU	A00126:224:H7HMWDSX3:3:1108:7744:2050997Test_seq16933360151M=170144835GGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:F,FFFFF:F:FF:NM:i:1MD:Z:134G16MC:Z:127S24MAS:i:146XS:i:109Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:7744:20509145Test_seq17014430127S24M=169333-835CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCAATTGTCCCTTTTATCATTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,169626,-,121M30S,60,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:7744:205092193Test_seq16962660121M30H=169333-414CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:4MD:Z:2T11A22G70A12MC:Z:151MAS:i:103XS:i:0SA:Z:Test_seq,170144,-,127S24M,30,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1108:7744:20509	Test_seq	169333	Test_seq	170167	+	-	RU	A00126:224:H7HMWDSX3:3:1108:7744:2050997Test_seq16933360151M=170144835GGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:F,FFFFF:F:FF:NM:i:1MD:Z:134G16MC:Z:127S24MAS:i:146XS:i:109Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:7744:205092193Test_seq16962660121M30H=169333-414CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:4MD:Z:2T11A22G70A12MC:Z:151MAS:i:103XS:i:0SA:Z:Test_seq,170144,-,127S24M,30,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:7744:20509145Test_seq17014430127S24M=169333-835CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCAATTGTCCCTTTTATCATTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,169626,-,121M30S,60,4;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1108:16233:21339	Test_seq	197163	Test_seq	197474	+	-	UU	A00126:224:H7HMWDSX3:3:1108:16233:21339161Test_seq19716360151M=197343312TCACATTTTTCTCCCAGCATACGCATGCTGATGTGGGGTTTTGTCATGTTTCTTAAGGGAACTGTGATGAAAATTCTCCTCTGACAGACATGTCTCAACTTCCAGATGGTAAGAAATTTTGTGAGAGTGATGAAATTAAGATACTTGATGCFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFF:F:FFFFFFFFFFFFFFF,FFFFFFFFFF:,F:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:16233:2133981Test_seq19734360132M19S=197163-312ATTCTAGGGGGAATTGCTTCCATGACAGTGTATTGAACCTGTGCTCCAAGGGGTGCCTTTAACAGTGGACTTGGTATCCTCTGGAGTTGTACAACGTAATTGCTCTGAGCCCAGAGCCCAGGAAATAAGATCGATCTTGATTTTCAGTACGFFF:,FF:FFFFFFF:FFFF:FFFFF:FFF:FFFFF:::FFFFFFF:F,FF:FFF::FF:FF,FFFFFF:FFFFFFFFFFFFFFFFFF:FFF::FF:FFFFFFFFFFFFFF::FFF:FF:FFF,FFFF:FFFFFFFFFF,FF:FFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:16342:23437	!	0	Test_seq	171131	-	+	NR	A00126:224:H7HMWDSX3:3:1108:16342:23437145Test_seq17129460128M23S=171131-291TAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTTAATGGATGTGTGCAGCTTTTCT:FFF:,FFFFFFFFFF:FFF,FFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FF:FF:FFF:FFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFF:FFFFF:FFFFFNM:i:2MD:Z:106G15T5MC:Z:151MAS:i:118XS:i:43Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:16342:2343797Test_seq17113160151M=171294291GCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTATCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFF::FFFF,FF,:FFF,FFFFFFFFFFFFF:FFFFFFFF,FF:F:FFF,FFFFF:FFFFF:FF:NM:i:1MD:Z:71C79MC:Z:128M23SAS:i:146XS:i:75Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1108:16740:23625	Test_seq	170381	Test_seq	170830	+	-	UU	A00126:224:H7HMWDSX3:3:1108:16740:23625161Test_seq17038160151M=170680450CTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGCGACGTTTTTCF:FFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::F:FFFFF:F,FF:FFFFFFF:FF:NM:i:1MD:Z:140A10MC:Z:151MAS:i:146XS:i:47Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:16740:2362581Test_seq17068060151M=170381-450GCGTTCTATATCACCTTGTATCACTCTCCATCTGGTAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGTTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAAF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:4MD:Z:0T4G29C15G99MC:Z:151MAS:i:135XS:i:0Yt:Z:UU
@@ -339,7 +339,7 @@
 A00126:224:H7HMWDSX3:3:1109:31367:22592	Test_seq	167451	Test_seq	213168	+	+	UU	A00126:224:H7HMWDSX3:3:1109:31367:22592129Test_seq1674515481M4I66M=21316845718TTCATCATTTTTGGTAATGTTTTGTGTTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATGCACTTGGAGTTAGTTTTTGTGCATGGFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFF:FFFFFF,F:FFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:25A36A0T56C26MC:Z:151MAS:i:117XS:i:87Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:31367:2259265Test_seq2131687151M=167451-45718AAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:144A6MC:Z:81M4I66MAS:i:146XS:i:141XA:Z:Test_seq,-167720,151M,2;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:14751:22748	Test_seq	171454	Test_seq	171939	+	-	UU	A00126:224:H7HMWDSX3:3:1109:14751:2274897Test_seq1714546028M1I122M=171789486GTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTCGATTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTFFFFFFFFFF,FFF,:FFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FF:FFFFFFFFFFF:FF:FFFFFFFFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FNM:i:6MD:Z:71A2T19A34T9T10MC:Z:151MAS:i:118XS:i:32Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:14751:22748145Test_seq17178960151M=171454-486GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTG:FFF::FFFFFFFFF:FFFFFF,F,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFFFFFFF:,FFF:FFFFFFFFFFFFNM:i:1MD:Z:149C1MC:Z:28M1I122MAS:i:149XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:32579:24314	Test_seq	132878	Test_seq	133237	+	-	UU	A00126:224:H7HMWDSX3:3:1109:32579:2431497Test_seq13287860151M=133104360GACGAGCCTTGAACGTGTGAGGCTCAGTACTGCTGTGTCTTTCACGTGCGCCGCTGTATTTGTGGCCTCTGTCTCCAGGGGAAGACTGTATTCGCAGCCCCTCATTCTCAGGGTACAACATTTTGTCTTCCGAGCAGCTGGATGTGAAGGCFFFFFFFFF:FFFFFFFFFFFFF,FFFFFF:FFFFFFFF,FFFFFF:FFFFFFFFFF,F:FFFFFFF,FFF,F:FFFFFFFF,FFF:FF:,FFFFFFFFFFFFFF:FFFFFFF:,F,,FF:F:FFFF:FFFFFFF:FF:FFF,FFF,FFFFNM:i:0MD:Z:151MC:Z:134M17SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:32579:24314145Test_seq13310460134M17S=132878-360TCAAACACTTATACACCAAGTCCTCACTTATCAACATGTTTAGGTTCCAAAGTCTAGGTTGTTATACAAAAATCGTCATTAAGCAAAAATGGTAGATGACTACAACATTACATAACTGCCAGACCACTAAGAATTAAAGTGACAGCTTGAAFFFFFFFFF:F::F,FF:F,FFFFF:F:,FFF::F::FF,,FFF,FFFF,:FFF:FFFFFFF:FFFFFFF:FFFFFFFF:,FFFF:FFFFFFFFFF,FFFF,F:FFF::FF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFF:F,FF:F:FFNM:i:1MD:Z:19T114MC:Z:151MAS:i:129XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1109:17246:27352	Test_seq	81985	Test_seq	97460	+	-	RU	A00126:224:H7HMWDSX3:3:1109:17246:2735297Test_seq8198560151M=9744015476TTTTTGTTATTGTGAACAATGCAGTGAACATTGGTGTGCATATGTCTGTTTGTGTGACGGCTCTTATTTCTCTAGGATATATTTCTAGGAATGGGATTGCTGGATCATACGGTATTTGTATTTGTAGCTTTTTAAGGAAGCACCATATCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:130S21MAS:i:151XS:i:81Yt:Z:RU	A00126:224:H7HMWDSX3:3:1109:17246:27352145Test_seq9744012130S21M=81985-15476GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGATCCAGTTCTTTTAGAAGCCFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,82184,-,132M19S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1109:17246:273522193Test_seq8218460132M19H=81985-331GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:21SA:Z:Test_seq,97440,-,130S21M,12,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1109:17246:27352	Test_seq	81985	Test_seq	97460	+	-	RU	A00126:224:H7HMWDSX3:3:1109:17246:2735297Test_seq8198560151M=9744015476TTTTTGTTATTGTGAACAATGCAGTGAACATTGGTGTGCATATGTCTGTTTGTGTGACGGCTCTTATTTCTCTAGGATATATTTCTAGGAATGGGATTGCTGGATCATACGGTATTTGTATTTGTAGCTTTTTAAGGAAGCACCATATCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:130S21MAS:i:151XS:i:81Yt:Z:RU	A00126:224:H7HMWDSX3:3:1109:17246:273522193Test_seq8218460132M19H=81985-331GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:21SA:Z:Test_seq,97440,-,130S21M,12,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1109:17246:27352145Test_seq9744012130S21M=81985-15476GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGATCCAGTTCTTTTAGAAGCCFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,82184,-,132M19S,60,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1109:17788:27821	Test_seq	168737	Test_seq	169220	+	-	UU	A00126:224:H7HMWDSX3:3:1109:17788:27821161Test_seq16873760151M=169070484TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGACCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTFFF,FFFFFFFFFFFF:FFFFFFF:FFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:F,FFFFFFFFFFFF:FFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FF,F,FFF:FFFFF::FFFNM:i:1MD:Z:38T112MC:Z:151MAS:i:146XS:i:81Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:17788:2782181Test_seq16907060151M=168737-484TTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAGTTTTACATAGTAFFFFFFFF::,FFFFFFFFFFFFFFF:FFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFNM:i:7MD:Z:84G2T3G7C31C6A6G5MC:Z:151MAS:i:116XS:i:66Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:12346:31078	Test_seq	171229	Test_seq	171627	+	-	UU	A00126:224:H7HMWDSX3:3:1109:12346:31078161Test_seq17122960151M=171476399TTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATCATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACT:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFF::FFF:FFFFFF:F:FFFFFF:FF:FFFFFFFFFFFFFNM:i:1MD:Z:114G36MC:Z:19M1D132MAS:i:146XS:i:27Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:12346:3107881Test_seq1714766019M1D132M=171229-399TCTTCATTTTTTTAAATTCTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTTCTCTGACTTTCTATTGTGTTGTCTAATTCTGTAATTTTATTGTT:FFFFFFFFF:FFFFFF:FFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:13T5^T29A74C27MC:Z:151MAS:i:129XS:i:26Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:30852:31782	Test_seq	166084	Test_seq	166553	+	-	UU	A00126:224:H7HMWDSX3:3:1109:30852:3178297Test_seq16608460151M=166403470TCAGGAGTAGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTGGGTCTCTGGTTCATGTGGCCCTTTCTATCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF,FF,FFF,FFFFFFFFF,:FF,FFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:8T77A26G26G10MC:Z:151MAS:i:131XS:i:71Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:30852:31782145Test_seq16640360151M=166084-470CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCF:FFFFFF:FFFFFFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF,FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:102T48MC:Z:151MAS:i:146XS:i:40Yt:Z:UU
@@ -353,7 +353,7 @@
 A00126:224:H7HMWDSX3:3:1110:12020:6057	!	0	Test_seq	171156	-	+	NR	A00126:224:H7HMWDSX3:3:1110:12020:605781Test_seq17135060121M30S=171156-315GGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTATGAGGATGGTGTTTCATTCTCTTGTACTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:50G15T54MC:Z:151MAS:i:111XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1110:12020:6057161Test_seq17115660151M=171350315CTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:121M30SAS:i:151XS:i:35Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1110:15130:6151	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1110:15130:615165Test_seq333515062M=38398650472CCTTTGCATAGTCAATAAAACACAGGTAAACATCCTTCTGGTATTCTCTGCCTTCAGCCAGGFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFF,F::FFFFFFFFF:FF,F,:,FNM:i:1MD:Z:51T10MC:Z:62MAS:i:57XS:i:57XA:Z:Test_seq,-383986,62M,1;Test_seq,+15151,62M,2;Test_seq,+316240,19M2I41M,3;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1110:15130:6151129Test_seq383986062M=333515-50472CCTGGCTGAAGGCAGAGAATACCAGAAGGATGTTTACCTGTGTTTTATTGACTATGCAAAGGFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FF:FFFFF,FFFF::FFFFFF,F:FFF:F:FFNM:i:1MD:Z:10A51MC:Z:62MAS:i:57XS:i:57XA:Z:Test_seq,-333515,62M,1;Test_seq,-15151,62M,2;Test_seq,-316240,19M2I41M,3;Test_seq,+449331,62M,4;Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1110:18936:8046	Test_seq	168392	Test_seq	168721	+	-	UU	A00126:224:H7HMWDSX3:3:1110:18936:8046161Test_seq16839260151M=168571330TCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCATGATGTTGAATAAGAGCGGTGATAAGGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFNM:i:6MD:Z:2T52T1C24A20T44A2MC:Z:151MAS:i:125XS:i:73Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:18936:804681Test_seq16857160151M=168392-330ATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAAFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFNM:i:1MD:Z:145T5MC:Z:151MAS:i:146XS:i:73Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1110:19090:8406	!	0	Test_seq	169404	-	-	MR	A00126:224:H7HMWDSX3:3:1110:19090:8406177Test_seq523240127S24M=169254117058AGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGATGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAACTCTCTGTCTCTTTATTGGTGCAFFFFFFFFF:F,,FFFFFF:FF::F,F,FFFF:FFFF:F:FFFFFFF,FFFF:FF,F:FFF,FFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:24SA:Z:Test_seq,168994,+,22S129M,60,1;XA:Z:Test_seq,-154647,127S24M,0;Test_seq,+255573,24M127S,0;Test_seq,+38332,24M127S,0;Test_seq,-170380,127S24M,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1110:19090:84062209Test_seq1689946022H129M=169254411GTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTFFFFFFFFFFFFFFFFFFF,FFFFF:FF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFF,FFF:F,FF:FFFF,FFFFFFF:F:FFFF:FFFF,F,F::FF:FFFFFF,,F:FFFFFFFFFNM:i:1MD:Z:44G84MC:Z:151MAS:i:124XS:i:49SA:Z:Test_seq,52324,-,127S24M,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1110:19090:8406113Test_seq16925460151M=52324-117058TGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFF:FFFFFFF:FFFFFFFF:FFFFF:F:FFFF:FFFFFFFFFF:FF:FFFFFFFFF:FFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFF:FF,F:FFNM:i:1MD:Z:14T136MC:Z:127S24MAS:i:146XS:i:82Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1110:19090:8406	!	0	Test_seq	169404	-	-	MR	A00126:224:H7HMWDSX3:3:1110:19090:84062209Test_seq1689946022H129M=169254411GTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTFFFFFFFFFFFFFFFFFFF,FFFFF:FF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFF,FFF:F,FF:FFFF,FFFFFFF:F:FFFF:FFFF,F,F::FF:FFFFFF,,F:FFFFFFFFFNM:i:1MD:Z:44G84MC:Z:151MAS:i:124XS:i:49SA:Z:Test_seq,52324,-,127S24M,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1110:19090:8406177Test_seq523240127S24M=169254117058AGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGATGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAACTCTCTGTCTCTTTATTGGTGCAFFFFFFFFF:F,,FFFFFF:FF::F,F,FFFF:FFFF:F:FFFFFFF,FFFF:FF,F:FFF,FFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:24SA:Z:Test_seq,168994,+,22S129M,60,1;XA:Z:Test_seq,-154647,127S24M,0;Test_seq,+255573,24M127S,0;Test_seq,+38332,24M127S,0;Test_seq,-170380,127S24M,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1110:19090:8406113Test_seq16925460151M=52324-117058TGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFF:FFFFFFF:FFFFFFFF:FFFFF:F:FFFF:FFFFFFFFFF:FF:FFFFFFFFF:FFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFF:FF,F:FFNM:i:1MD:Z:14T136MC:Z:127S24MAS:i:146XS:i:82Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1110:18765:8531	Test_seq	169027	Test_seq	169502	+	-	UU	A00126:224:H7HMWDSX3:3:1110:18765:853197Test_seq16902760151M=169352476GCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFF,FFF:FFFFFFFFFF:FFF:FFFFFF::FFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF,F:FF:FF,FFFFF:FF,:FNM:i:5MD:Z:14T23A57A37G1A14MC:Z:151MAS:i:126XS:i:61Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:18765:8531145Test_seq16935260151M=169027-476TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGFFFFFFFFF:FFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:115G35MC:Z:151MAS:i:146XS:i:101Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1110:23927:10676	Test_seq	166048	Test_seq	166495	+	-	UU	A00126:224:H7HMWDSX3:3:1110:23927:10676161Test_seq16604860151M=166345448AATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCTTTTCTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:143C7MC:Z:151MAS:i:146XS:i:45Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:23927:1067681Test_seq16634560151M=166048-448TTTTCATAATAGAATTATTTTGCCCATTGACTTAGAAGTCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAGGCATTCATTTTATCCTGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:6MD:Z:25G14T10T38A2T12C44MC:Z:151MAS:i:121XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1110:29161:13416	Test_seq	169352	Test_seq	169778	+	-	UU	A00126:224:H7HMWDSX3:3:1110:29161:1341697Test_seq16935260151M=169628427TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFF:FFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,FFFFFFFFFFFF,FFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:66Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:29161:13416145Test_seq16962860151M=169352-427CGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATGTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCCTTGTGGTCTGAFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:0T34G54T28C31MC:Z:151MAS:i:135XS:i:62Yt:Z:UU
@@ -393,7 +393,7 @@
 A00126:224:H7HMWDSX3:3:1111:27254:10113	!	0	Test_seq	169321	-	+	NR	A00126:224:H7HMWDSX3:3:1111:27254:10113145Test_seq16962860119M32S=169321-426CGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCTCTTTATTTGCACTGCTTTAGGFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFNM:i:4MD:Z:0T11A22G70A12MC:Z:151MAS:i:103XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1111:27254:1011397Test_seq16932160151M=169628426AGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,:FFFF:F,FFFF,:FF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:146G4MC:Z:119M32SAS:i:146XS:i:112Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1111:22200:10222	Test_seq	117403	Test_seq	117847	+	-	UU	A00126:224:H7HMWDSX3:3:1111:22200:10222161Test_seq11740360151M=117697445AATCCACTTAAGTATTTGAATGTTATTTCAACCTCTTTCTATGAGGAAGAGCTTTGGGAAAAAATAAGTAGAAAGTGAAAATATCGATAGTGATTAATGAGTGATGGCTGGCTTTGACTGCTGTAGGAGTAGGTAGGACAACCGCTACAAAFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFF,FF:FFFF,FFFFFFFFFFFFFFF,F:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFF,FF:FFFFFFFF:F:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:148G2MC:Z:151MAS:i:148XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:22200:1022281Test_seq11769760151M=117403-445TCCTCTTTCTGTGGGATGGACAAGGATGGAGCCTAGGGCCCCATCCCTGTCACATTTCTGCACAGTCCTCAGACACTTGCAGAAAGTGTTGCCGGTTTTTAGCACATCTTTCTCTGGAGATGAAACTGGCTCAGCACCAACCAGAGGGCACFFFFFF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:17499:11537	Test_seq	171298	Test_seq	171659	+	-	UU	A00126:224:H7HMWDSX3:3:1111:17499:1153797Test_seq17129860151M=171509362TGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFF:F:FFFFFF,FF:F,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFF,F:FFFFFFFFFF:NM:i:3MD:Z:62G39G15T32MC:Z:151MAS:i:136XS:i:61Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:17499:11537145Test_seq17150960151M=171298-362TTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:47Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1111:19994:11788	Test_seq	20289	Test_seq	168429	-	+	UR	A00126:224:H7HMWDSX3:3:1111:19994:11788145Test_seq202637124S27M=168429148141TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCTTTTCTTTCTTTTTAATGACCTCTTGCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:6G20MC:Z:151MAS:i:22XS:i:20SA:Z:Test_seq,168657,-,120M31S,60,3;XA:Z:Test_seq,-55966,131S20M,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1111:19994:117882193Test_seq16865760120M31H=168429-348TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:3MD:Z:18C30T9T60MC:Z:151MAS:i:105XS:i:50SA:Z:Test_seq,20263,-,124S27M,7,1;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1111:19994:1178897Test_seq16842960151M=20263-148141CCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFF:FF,FFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,NM:i:1MD:Z:66T84MC:Z:124S27MAS:i:146XS:i:78Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1111:19994:11788	Test_seq	20289	Test_seq	168429	-	+	UR	A00126:224:H7HMWDSX3:3:1111:19994:117882193Test_seq16865760120M31H=168429-348TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:3MD:Z:18C30T9T60MC:Z:151MAS:i:105XS:i:50SA:Z:Test_seq,20263,-,124S27M,7,1;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1111:19994:11788145Test_seq202637124S27M=168429148141TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCTTTTCTTTCTTTTTAATGACCTCTTGCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:6G20MC:Z:151MAS:i:22XS:i:20SA:Z:Test_seq,168657,-,120M31S,60,3;XA:Z:Test_seq,-55966,131S20M,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1111:19994:1178897Test_seq16842960151M=20263-148141CCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFF:FF,FFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,NM:i:1MD:Z:66T84MC:Z:124S27MAS:i:146XS:i:78Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1111:20690:11929	!	0	Test_seq	167597	-	-	NR	A00126:224:H7HMWDSX3:3:1111:20690:1192997Test_seq1672982124S50M1I76M=167447300TTTGAAGGCTTGTCTTGTGAATTTAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGACATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFNM:i:3MD:Z:25A44A55MC:Z:151MAS:i:109XS:i:99XA:Z:Test_seq,-213611,74M1I52M24S,5;Test_seq,+400719,24S50M1I76M,7;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1111:20690:11929145Test_seq16744760151M=167298-300TCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:24S50M1I76MAS:i:151XS:i:101Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1111:24795:12649	Test_seq	168660	Test_seq	212799	-	-	UU	A00126:224:H7HMWDSX3:3:1111:24795:12649113Test_seq16851060151M=21264944140TGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:126A24MC:Z:151MAS:i:146XS:i:78Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:24795:12649177Test_seq21264925151M=168510-44140GGCAAGGAAGAAGTAAAGTTATCACCATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCAACAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:10MD:Z:8G8A5T1T18C0T17T8T0C51G25MC:Z:151MAS:i:101XS:i:86XA:Z:Test_seq,+168239,151M,13;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:8802:14732	Test_seq	170230	Test_seq	170661	+	-	UU	A00126:224:H7HMWDSX3:3:1111:8802:14732161Test_seq17023060151M=170511432AATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATCTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGAFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFNM:i:1MD:Z:47T103MC:Z:151MAS:i:146XS:i:72Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:8802:1473281Test_seq17051160151M=170230-432TTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTTGTTACGTTTTTCTTGAGTTATGGAATCGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:76C26T47MC:Z:151MAS:i:141XS:i:37Yt:Z:UU
@@ -424,7 +424,7 @@
 A00126:224:H7HMWDSX3:3:1111:1570:34773	Test_seq	72280	Test_seq	72638	+	-	UU	A00126:224:H7HMWDSX3:3:1111:1570:3477397Test_seq7228060151M=72488359ATCAGTCTGTTTTGAGATAGAAAAGAGATTAAACAAGGAGATTAAAGAAGCAGCGATGGAGGAAGAGAGATGCAAGGCCGCATGAAGGTCGCCCAAGAGCAGAAGCTCAAAAGAGGCTAAGGTCTTCCTCCAGAGCTGAGATAGAAAGCTTFFFF:FFFFFFFFFFFFFF:FFFFF::FFFFF:FFF:FFFFFFFF:FFFFFFFFFF:FF:FFFF::FF:FFF:FFFF,FFFFFFFFFFFF::FFFF::FFFF:FFFFFFF,F:,FF:FF:FFFFFFFF:FFFFFF:FFFFFFFFFF:FF:FNM:i:1MD:Z:137A13MC:Z:151MAS:i:146XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:1570:34773145Test_seq7248860151M=72280-359AAATTTGTATGTTAAAACCGCCTACTTGTGGTATTTCTGTTATAGAACACTGGATAACTAGGACAGTTGCCGTGAAGGGGCAGCCGACTCGATGGCAGCCAAAAACAACAACAACAGCAGTGAAACACAGGTAGTGTTTCTTGGATTCATTF:FFFFFFF:,F:FFFFFF:,FFFFFF,F,F:,FF:FFFFFFFFFFFFFFF,FFFFFFFF:FFFF:FFFFFFFF:FFFFFFF:F,FFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFF:F:::FF:FFFFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:14064:36808	Test_seq	167148	Test_seq	401034	+	-	UU	A00126:224:H7HMWDSX3:3:1111:14064:3680897Test_seq16714842151M=400900233887AATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF,,F:FFF,,FFF:FFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFNM:i:1MD:Z:95T55MC:Z:135M16SAS:i:146XS:i:126XA:Z:Test_seq,-213736,151M,5;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:14064:36808145Test_seq4009004135M16S=167148-233887TTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCCTTCCCCACTC,FF:FFFF,FFFFFFFF,FFFF:FF:FF,F:FFFFFFFFF:FFFFF:FF:FFF:FFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFF:FFFFFFFF:FFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:FFFF,F:FNM:i:7MD:Z:4T0T11G32G19A0C8T54MC:Z:151MAS:i:104XS:i:100XA:Z:Test_seq,-167437,135M16S,7;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:23149:2597	Test_seq	169079	Test_seq	169468	+	-	UU	A00126:224:H7HMWDSX3:3:1112:23149:259797Test_seq1690793739M4I108M=169318390CCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGATCGATCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGFFF:FFF:FFFFF,FFFFFFFFFFFFFFF:F:FFFFFFFFFFF,FFFFFFF:FF:F:F:F::FFFFFF:FFFFF:FFFFFFF:FFFFF,FFF,F:FFFFFFFFFFFFFFF,FF:F:FFFFFFF:FF,FF,FFFFFF,:,,F:F:,F:F,:FNM:i:12MD:Z:40T3A37G1A37C11A1G2G7MC:Z:151MAS:i:99XS:i:76Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:23149:2597145Test_seq16931860151M=169079-390GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATFFFFFFFFFFFFFFFFFF,FFFF:FFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:1MD:Z:149G1MC:Z:39M4I108MAS:i:149XS:i:112Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1112:10447:5243	Test_seq	168427	Test_seq	168802	+	-	RU	A00126:224:H7HMWDSX3:3:1112:10447:5243161Test_seq16842760151M=168773376GCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCAGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:68T82MC:Z:121S30MAS:i:146XS:i:43Yt:Z:RU	A00126:224:H7HMWDSX3:3:1112:10447:524381Test_seq16877360121S30M=168427-376TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCGATCAGGGTGAATTATTTTTTTGATGTGTTFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,168555,-,121M30S,60,1;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1112:10447:52432129Test_seq16855560121M30H=168427-249TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A95MC:Z:151MAS:i:116XS:i:70SA:Z:Test_seq,168773,-,121S30M,60,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1112:10447:5243	Test_seq	168427	Test_seq	168802	+	-	RU	A00126:224:H7HMWDSX3:3:1112:10447:5243161Test_seq16842760151M=168773376GCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCAGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:68T82MC:Z:121S30MAS:i:146XS:i:43Yt:Z:RU	A00126:224:H7HMWDSX3:3:1112:10447:52432129Test_seq16855560121M30H=168427-249TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A95MC:Z:151MAS:i:116XS:i:70SA:Z:Test_seq,168773,-,121S30M,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1112:10447:524381Test_seq16877360121S30M=168427-376TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCGATCAGGGTGAATTATTTTTTTGATGTGTTFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,168555,-,121M30S,60,1;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1112:21884:7012	Test_seq	167977	Test_seq	168320	+	-	UU	A00126:224:H7HMWDSX3:3:1112:21884:701297Test_seq16797726151M=168170344AGTGCGTTAAATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGCAGTAGAGCTTTGTAGTTTTCTTTGTATAGGTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFNM:i:8MD:Z:9T64G8C32T2C4T3A13G8MC:Z:151MAS:i:111XS:i:96XA:Z:Test_seq,-212911,141M10S,9;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:21884:7012145Test_seq16817031151M=167977-344TGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:101T49MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,+212718,151M,4;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:16595:8124	Test_seq	170144	Test_seq	170457	+	-	UU	A00126:224:H7HMWDSX3:3:1112:16595:8124161Test_seq1701446016S135M=170307314TTTTATGTTATTGATCGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:FFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFF:FFF,FFFFFFFF:FFFFFF:F,FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFF,FNM:i:3MD:Z:51C0A65A16MC:Z:151MAS:i:120XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:16595:812481Test_seq17030760151M=170144-314TGTGTCTCTAAGTCTACGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:16A134MC:Z:16S135MAS:i:146XS:i:48Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:9209:9361	Test_seq	212679	Test_seq	212953	+	-	UU	A00126:224:H7HMWDSX3:3:1112:9209:936197Test_seq21267960151M=212803275GCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:110Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:9209:9361145Test_seq21280358151M=212679-275TCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGAGAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAACTACAAAGCACTACTACAAGAAATFFFFFFF:FFFFFFFFFFFFFFFFFFFF:::FFFF,F,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:5MD:Z:34A37T54T7T14C0MC:Z:151MAS:i:130XS:i:100Yt:Z:UU
@@ -436,7 +436,7 @@
 A00126:224:H7HMWDSX3:3:1112:5927:14763	Test_seq	366670	Test_seq	367055	+	-	UU	A00126:224:H7HMWDSX3:3:1112:5927:1476397Test_seq36667060151M=366905386AGTGTTAAAAAGCAAAGATGTTACCTTGAAGACTAAGGTGTGCCTGACCGAAAAAAAGACCGAAGCCATGGTATTTTCAATTGCATCATATGCATGTGAAAGCTGGACAATGAATGAGAAAGGCCAAAGAAGAATTGATGTTTTTGAATTAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:50Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:5927:14763145Test_seq36690560151M=366670-386TCCTTAGAAGCAAGATTGTCAAGACTTTGATGTACTTTGGGCATGTTATCAGGAAGGATTCATCCCTAGAGAAGGACACCATGCTTGGTAAAATAGAAAAAAACCAAAACCAAACCCACTGCCGTCAAGTGATTCCGACTCATAGCGATCTF:FFFFFFF::FF:FFFFFFFF:F,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF,FFFF,FFFFFFFF,FFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:61G89MC:Z:151MAS:i:146XS:i:22Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:9254:15922	Test_seq	260973	Test_seq	261025	+	-	UU	A00126:224:H7HMWDSX3:3:1112:9254:1592297Test_seq260973853M=26097353GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCTGTTGTAGAAGGGCACFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FF:FFFF,,,FFFFFNM:i:3MD:Z:37G7C0T6MC:Z:53MAS:i:40XS:i:36XA:Z:Test_seq,+347640,45M8S,2;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:9254:15922145Test_seq2609733453M=260973-53GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCGGTTGTAGCTGGGCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:53MC:Z:53MAS:i:53XS:i:43XA:Z:Test_seq,-150325,53M,2;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:32723:16391	!	0	Test_seq	171881	-	-	NR	A00126:224:H7HMWDSX3:3:1112:32723:16391161Test_seq1716266027S124M=171731256TTCAATAAAAACCCAAATGAAATCGATTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTGTTAATTTTTCCACTATGTTCTTGAATAATCTTTTTGAGTTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF:,FFFFFF,F:,FFFFF:FFFFFFFF:F,FFFFFFFFF:FF,FF,FFFFFF,FFFFFFFFFF,,:F:,FFFFF,F:FF:,,F,FFFNM:i:4MD:Z:50A14C13C11T32MC:Z:151MAS:i:104XS:i:71Yt:Z:NR	A00126:224:H7HMWDSX3:3:1112:32723:1639181Test_seq17173160151M=171626-256TCAGTGTGTTCCTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGF,FF:FFF,FFF:FFFFFFFFFFFFFFF:F:FFFF,FFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFF,FFFFFFF:F:F:,:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFF:,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:1MD:Z:27G123MC:Z:27S124MAS:i:146XS:i:25Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1112:27633:17722	Test_seq	385678	Test_seq	388748	-	-	UR	A00126:224:H7HMWDSX3:3:1112:27633:17722113Test_seq38565530127S24M=3885983071AAGTGCGTCAGAAGAAAGTCTGCAAAGCATTTCAGAGAGCACTCAAAGAGAAGCCTTTTAAGGAAAACTCTGAACAAAAATTATCCTCATAAGATAATTCTCTCTTCAACACTCCTCATTCTGGAGTAATCACAGACTGTTCACACATTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,388349,+,24S127M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1112:27633:177222145Test_seq3883496024H127M=388598400ACTCCAGAATGAGGAGTGTTGAAGAGAGAATTATCTTATGAGGATAATTTTTGTTCAGAGTTTTCCTTAAAAGGCTTCTCTTTGAGTGCTCTCTGAAATGCTTTGCAGACTTTCTTCTGACGCACTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0SA:Z:Test_seq,385655,-,127S24M,30,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1112:27633:17722177Test_seq38859860151M=385655-3071AGATTGGCCATGAATGGGGTGATAAGAATAGGGAGAAGCACATACCAGTAGACCAGACTTCCTGTGCACAAGCCTTTGAATCCTGCTTCTCGCTTCTGAAATAGCATTCATCTTAGGGCTGGGATCATTTATTCTCTCTGGAATTAAGCAAFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1112:27633:17722	Test_seq	385678	Test_seq	388748	-	-	UR	A00126:224:H7HMWDSX3:3:1112:27633:177222145Test_seq3883496024H127M=388598400ACTCCAGAATGAGGAGTGTTGAAGAGAGAATTATCTTATGAGGATAATTTTTGTTCAGAGTTTTCCTTAAAAGGCTTCTCTTTGAGTGCTCTCTGAAATGCTTTGCAGACTTTCTTCTGACGCACTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0SA:Z:Test_seq,385655,-,127S24M,30,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1112:27633:17722113Test_seq38565530127S24M=3885983071AAGTGCGTCAGAAGAAAGTCTGCAAAGCATTTCAGAGAGCACTCAAAGAGAAGCCTTTTAAGGAAAACTCTGAACAAAAATTATCCTCATAAGATAATTCTCTCTTCAACACTCCTCATTCTGGAGTAATCACAGACTGTTCACACATTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,388349,+,24S127M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1112:27633:17722177Test_seq38859860151M=385655-3071AGATTGGCCATGAATGGGGTGATAAGAATAGGGAGAAGCACATACCAGTAGACCAGACTTCCTGTGCACAAGCCTTTGAATCCTGCTTCTCGCTTCTGAAATAGCATTCATCTTAGGGCTGGGATCATTTATTCTCTCTGGAATTAAGCAAFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1112:25825:18098	!	0	Test_seq	171611	-	-	NR	A00126:224:H7HMWDSX3:3:1112:25825:18098161Test_seq1711746033S118M=171461438TATATTGAGTATATCATCATTTGACCTCAAAGAATTCTCCCTTGAAGGAAACCTTTTTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFFFFFFF,F:FFFFFFFFFNM:i:1MD:Z:23C94MC:Z:151MAS:i:113XS:i:35Yt:Z:NR	A00126:224:H7HMWDSX3:3:1112:25825:1809881Test_seq17146160151M=171174-438ATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:122T28MC:Z:33S118MAS:i:146XS:i:39Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1112:9362:18740	Test_seq	170285	Test_seq	170742	+	-	UU	A00126:224:H7HMWDSX3:3:1112:9362:18740161Test_seq17028560151M=170592458ATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCCTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGAFFF,FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:,FF::FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFF:FFFF:FFFFFFFFF:FFFFF:FFFF,F::FFF,F:FFFFFF,F,:FFFFFFFFF,FFF,FFFFFNM:i:1MD:Z:107T43MC:Z:151MAS:i:146XS:i:82Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:9362:1874081Test_seq17059260151M=170285-458CTTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCGATGCCTCCTATATTTAGTCCCFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,FFFFFFFF:FFFF,FFFFF:F,FFFFFF:FFFFF:FFFF:FFFFFFF,FFFFFFFFFNM:i:8MD:Z:1G45A42G2G3A2A28A9G11MC:Z:151MAS:i:114XS:i:24Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:31421:19304	Test_seq	236629	Test_seq	237126	+	-	UU	A00126:224:H7HMWDSX3:3:1112:31421:1930497Test_seq23662960151M=236976498AAGAAAAATAAAAGTAAATTCCACAGTAAGGTGAACAATTAAGATGAGATTACCAACTGAGATTACCCATTTCAGAAAGAAGTATTCTAAGTAAAAGGTAATCAAAAGTTGGCTTTTCTACTTTCAATAGAGGATTCACACATAGTCATTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:31421:19304145Test_seq23697660151M=236629-498TAAAATACATGCAGCAGAAGGTTAGTACGTAAAGACTAATTCAAACTATGCACTGATGTGTTGTTGTTAGCTGCTGTCAGGTTGGCCCTGACTCATGGTGATGCCACGCACAATGGAACAAAAGGCTGCCAGGTCCTGTGTTATATCCCATFFF:::FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:28Yt:Z:UU
@@ -470,7 +470,7 @@
 A00126:224:H7HMWDSX3:3:1113:4526:3975	Test_seq	168585	Test_seq	212883	-	-	UU	A00126:224:H7HMWDSX3:3:1113:4526:3975113Test_seq16843560151M=21273344299TAGGACTCCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:7T143MC:Z:151MAS:i:146XS:i:78Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:4526:3975177Test_seq21273320151M=168435-44299CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTF:FF:FFFFFFF::FFFFFFF,:FFF:,F,FFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFF,:FFFF,F:FFFFFFFFFF:FF:FFFFFF:F,FFFFFFFFF:::FFFFF,FFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFNM:i:3MD:Z:41G51A10A46MC:Z:151MAS:i:136XS:i:126XA:Z:Test_seq,+168155,151M,5;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1113:5565:7560	!	0	Test_seq	166317	-	+	NR	A00126:224:H7HMWDSX3:3:1113:5565:756081Test_seq16643460120M31S=166317-237AAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCTAAAATTCAAGTCATATCTTGAAATTCACTA:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:30Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:5565:7560161Test_seq16631760151M=166434237GCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTCCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFF:FFFFNM:i:1MD:Z:49G101MC:Z:120M31SAS:i:146XS:i:32Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:12644:7701	Test_seq	424202	Test_seq	424540	+	-	UU	A00126:224:H7HMWDSX3:3:1113:12644:770197Test_seq42420260151M=424407339TGGGGCAGAAGACTGCTGTTTTTCACACTTAGAGAATAAGACCTTTTCAAACTCAACATGTCCACTTGGATGTCTAATAGACATCTTAAACTTAATATATTTAAAACAATATTTGGCCCCAAAGCTGCCCTATTTTGTGTCAGCTGATGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:134M17SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:12644:7701145Test_seq42440760134M17S=424202-339ATATCCAATCCTTCAGGAAATCCTTTAGAATATCTCCAGACTCTGACCATCTTGCGCTTCCTCTGCTGTCACCATCCTGGGTCAAGCCCATCATCTCTCACCTGAACTCCTGCTCATAATCTCCTCCCAGATCTAAACTACACAGTATCTAFF:FFFFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FF:FFFFFFFFFNM:i:0MD:Z:134MC:Z:151MAS:i:134XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1113:13765:8735	Test_seq	167568	Test_seq	328153	+	+	RU	A00126:224:H7HMWDSX3:3:1113:13765:8735129Test_seq16756832151M=328153160586GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:2S25M124SAS:i:151XS:i:136XA:Z:Test_seq,-213320,140M2I9M,3;Yt:Z:RU	A00126:224:H7HMWDSX3:3:1113:13765:873565Test_seq328153192S25M124S=167568-160586AAGGAGCCCTGGTGGTGCAGTGGTTAGATCCAAATTCCAATGTCATATTTTAAGGGGATAGAGAAACAAATCACTAATTTCATATGGAAGGGAAAGAACCCCCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCAFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF,FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFNM:i:0MD:Z:25MC:Z:151MAS:i:25XS:i:21SA:Z:Test_seq,167849,-,124M27S,60,1;XA:Z:Test_seq,+141607,26M125S,1;Test_seq,+432045,26M125S,1;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1113:13765:87352129Test_seq16784960124M27H=167568-405TGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGAATTTGGATFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF,FFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:115T8MC:Z:151MAS:i:119XS:i:75SA:Z:Test_seq,328153,+,2S25M124S,19,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1113:13765:8735	Test_seq	167568	Test_seq	328153	+	+	RU	A00126:224:H7HMWDSX3:3:1113:13765:8735129Test_seq16756832151M=328153160586GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:2S25M124SAS:i:151XS:i:136XA:Z:Test_seq,-213320,140M2I9M,3;Yt:Z:RU	A00126:224:H7HMWDSX3:3:1113:13765:87352129Test_seq16784960124M27H=167568-405TGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGAATTTGGATFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF,FFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:115T8MC:Z:151MAS:i:119XS:i:75SA:Z:Test_seq,328153,+,2S25M124S,19,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1113:13765:873565Test_seq328153192S25M124S=167568-160586AAGGAGCCCTGGTGGTGCAGTGGTTAGATCCAAATTCCAATGTCATATTTTAAGGGGATAGAGAAACAAATCACTAATTTCATATGGAAGGGAAAGAACCCCCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCAFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF,FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFNM:i:0MD:Z:25MC:Z:151MAS:i:25XS:i:21SA:Z:Test_seq,167849,-,124M27S,60,1;XA:Z:Test_seq,+141607,26M125S,1;Test_seq,+432045,26M125S,1;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1113:8892:9878	!	0	Test_seq	397096	-	-	NR	A00126:224:H7HMWDSX3:3:1113:8892:9878161Test_seq3967016025S126M=396946396TTTTTTCTAGTATACCATTTTGATCAGTCTCTCCTGACATCCATTTTGATGTTTCTTTCCTGTCTTTTTAATGACCTTTTGCTTTGTTCATGTATGAGGTCCCTGATTTCATCCCACAGCTCATCTGGTCTTTGGTCATTAGTGTTCAGTGFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,::FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:8892:987881Test_seq39694660151M=396701-396CATCTGATGGTCTATTCTGCAGTCGGCTCCTGGCCTTGTTCTGACTGATGATATTGAGCTTCTTCATTGTCTCTTTCCACAGATGTAGTTGATTCGATTCCTGTGTATTCTATCTGGTGAGGTGTGTATAGTCACAGTTTATTTTGTTGAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:95A55MC:Z:25S126MAS:i:146XS:i:58Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:7084:10441	!	0	Test_seq	167640	-	-	NR	A00126:224:H7HMWDSX3:3:1113:7084:10441161Test_seq167316721S130M=167490325ATCTGACACATTTAAGTGATCGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFF:FFFNM:i:3MD:Z:7A44A73T3MC:Z:151MAS:i:116XS:i:111XA:Z:Test_seq,-213589,130M21S,4;Test_seq,+400737,21S123M7S,5;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:7084:1044181Test_seq16749060151M=167316-325CTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTACATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:1MD:Z:102G48MC:Z:21S130MAS:i:146XS:i:96Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:3025:10614	Test_seq	385792	Test_seq	386184	+	-	UU	A00126:224:H7HMWDSX3:3:1113:3025:10614161Test_seq3857926015S136M=386034393GCCAGAAGGTTCCTTAATCCCTATCCCAAGGGATTTATCTCTAAAAAGAAATGCTGTTTTGTGCGTGGAATTCAGTCTGACTCATAGCACCCTGTATGACGAAGTAGAACTGCCCCACACGGTTTCCTAGGCTGTGATATTTACAAAAAAAFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FF:FF:FFFF,FFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:,FFFFFFFFFF:FFFFFFF,FFFNM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:3025:1061481Test_seq38603460151M=385792-393CGTTTCTTCTGTTTTTGCTCCTCATCTTCTTGCCTATTTGAGACATCACAATTTGTTTGTGCAATCCTGGAAATTCTCCTTGTTTCCACCCTCAACCCATCTCAGCCTGCTGCCTTCTTGTAGACCAGGGTCCCTGACTCTGGCCCATGCA:FFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FFF:FFFFFFFFFFFFFFFFFFFFFFF:::FFF:FFFFF:FFF:FFFFFF,FFFF,FFF:FFFF::F:FF:F:FFFFFFFFFNM:i:0MD:Z:151MC:Z:15S136MAS:i:151XS:i:0Yt:Z:UU
@@ -507,9 +507,9 @@
 A00126:224:H7HMWDSX3:3:1114:11785:1485	Test_seq	170257	Test_seq	170704	+	-	UU	A00126:224:H7HMWDSX3:3:1114:11785:148597Test_seq17025760151M=170566448TTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAFFFFFFFF:FFFFFFFFFFF:F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFF:F:FFFFFFFF:FFFFFFFFF,F:FFF,FFF:FFFFFFFF:FF:F:FFF:FFFFFFFFFFF:FFFFFFFFFF,FF:FFF,FNM:i:1MD:Z:5A145MC:Z:32M12I107MAS:i:146XS:i:80Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:11785:1485145Test_seq1705666032M12I107M=170257-448TTGACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATGGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATGACTFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFF:FFF::F,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:20MD:Z:2A10T32A3A3T64G6A8C3MC:Z:151MAS:i:84XS:i:37Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:14986:3239	!	0	Test_seq	333153	-	+	NR	A00126:224:H7HMWDSX3:3:1114:14986:323981Test_seq33337760124M27S=333153-348CTGCAGTATTCCCTTGTTCTGTCTGACCAACTGCCTCTTGATCTATGTAAAGGTTCCTTATGAGCACAATTAAGTGTTCTGGAATTCCCATTCTTCGCAGTGTTATCCAGAGTTTGTTATGATCGATCCAGGCAGGTAAACCTTCAGGCCA,:FFFFFF,:FFF:FF:,FFFFF:F,:F:,FFF,:FF,F:,FFFFFF:F,FFF::FFFFFFFFFFFFF:FF,FFFFFFFFFF:F,:FFFFFFF:FFFF:FFFF:F:FFFFFFFFF::FF:F:FFFFFFF,F:FFFF,FFFFFFFFFF:FFFNM:i:1MD:Z:0A123MC:Z:151MAS:i:123XS:i:62Yt:Z:NR	A00126:224:H7HMWDSX3:3:1114:14986:3239161Test_seq33315332151M=333377348GTGGTCTTTGATCTTCATTAGTAAGTGCTTCAAGTCCTCTTCACTTTCAGCAAGCAAGGTTGTGTCATCTGCATAACGCAGGTTGTTAAAGAGTCTTCCTCCAAACCTGATGCCCCGTTCTTCTTCATATAGTCCAGCTTCTCGTATTATTF:FF,FFF:FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFF,FFFFFFFFF:FFF:F:FFFFFF:FFFF:FFFFFFF:F:FFFFFFFFFF:FFFFF:F,::::FFFF:FF:F,,F:FF,FFFFFF:,FFFFF:FFFFF,,:FF:FFFF,,F:NM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:136XA:Z:Test_seq,+315878,151M,3;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1114:26268:3333	Test_seq	435684	Test_seq	436086	+	-	UU	A00126:224:H7HMWDSX3:3:1114:26268:3333161Test_seq43568460151M=435936403CTGAGCAATAATCACCGCCCATGGGGCAGTTCTGCTCTGCCCTATAGGGTTGCTATGAGTCGGAATCAACTCCAGGGCAATAGGGTTTTTTTGGTTTTACCATCACCTTGAATCAGAAACAACTAGACAGCAATAGCAAATAGCTAAAAGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:31Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:26268:333381Test_seq43593660151M=435684-403TTATCGCAGTTGTAAATACCTTTTCTCTCTTTTTCAGGACAGACTGGCAAATCTTTGAATGTCTAATGTACACCTATGTATGATAATCTCTTTTCCTCCAGGAAAAAATTTCTGCCTTGAAAAAGCAAGCGAATCATATTGTCCAGCAGGCFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1114:5791:5008	Test_seq	70329	Test_seq	71380	-	-	UR	A00126:224:H7HMWDSX3:3:1114:5791:5008113Test_seq703106131S20M=712301052GAGACGTGGAGCCAACTTGGCCCAATTCAGTCAAGGTCACGTCCAATGATCACGTCACAGACACCAAATCCTCAGCCGCAAAAGCATGGCGAGAGGGCAGCATGCAGGTGGAAGCAGCCCATTCTAGGATCAATCCAGGTTCTGCCCCTCCFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,71226,+,20S131M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:5791:50082145Test_seq712266020H131M=71230155GATCCTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:0SA:Z:Test_seq,70310,-,131S20M,6,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1114:5791:5008177Test_seq7123060151M=70310-1052CTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCTCCCAGCCTCTAGGAGCTTATGAGFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:131S20MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1114:5791:5008	Test_seq	70329	Test_seq	71380	-	-	UR	A00126:224:H7HMWDSX3:3:1114:5791:50082145Test_seq712266020H131M=71230155GATCCTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:0SA:Z:Test_seq,70310,-,131S20M,6,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:5791:5008113Test_seq703106131S20M=712301052GAGACGTGGAGCCAACTTGGCCCAATTCAGTCAAGGTCACGTCCAATGATCACGTCACAGACACCAAATCCTCAGCCGCAAAAGCATGGCGAGAGGGCAGCATGCAGGTGGAAGCAGCCCATTCTAGGATCAATCCAGGTTCTGCCCCTCCFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,71226,+,20S131M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1114:5791:5008177Test_seq7123060151M=70310-1052CTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCTCCCAGCCTCTAGGAGCTTATGAGFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:131S20MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1114:24153:6778	Test_seq	166090	Test_seq	166553	+	-	UU	A00126:224:H7HMWDSX3:3:1114:24153:677897Test_seq16609060151M=166418464GTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,F:F:FFFFF,FF::FFFFFFFFFFFF:FFFFFFFFFF:FF,:NM:i:1MD:Z:134G16MC:Z:136M15SAS:i:146XS:i:85Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:24153:6778145Test_seq16641860136M15S=166090-464GCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAATCTCTTTGCTTTTAGTCCAGTCCAGTTGGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCGATCCACCGTCAATCFFFFFFF,FFFFFFFFFFFFFFFF:F:FF:FFFFFFFF,FFF:FFFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:39C23A2A20T48MC:Z:151MAS:i:116XS:i:40Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1114:29423:7827	Test_seq	15892	Test_seq	168477	-	+	UR	A00126:224:H7HMWDSX3:3:1114:29423:7827145Test_seq158736131S20M=168477152586TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCACCCTTTGAAATCTTCTGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,168685,-,126M25S,60,2;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:29423:78272193Test_seq16868560126M25H=168477-334TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:2A28T94MC:Z:151MAS:i:118XS:i:58SA:Z:Test_seq,15873,-,131S20M,6,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1114:29423:782797Test_seq16847760151M=15873-152586GCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18T132MC:Z:131S20MAS:i:146XS:i:92Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1114:29423:7827	Test_seq	15892	Test_seq	168477	-	+	UR	A00126:224:H7HMWDSX3:3:1114:29423:78272193Test_seq16868560126M25H=168477-334TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:2A28T94MC:Z:151MAS:i:118XS:i:58SA:Z:Test_seq,15873,-,131S20M,6,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:29423:7827145Test_seq158736131S20M=168477152586TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCACCCTTTGAAATCTTCTGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,168685,-,126M25S,60,2;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1114:29423:782797Test_seq16847760151M=15873-152586GCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18T132MC:Z:131S20MAS:i:146XS:i:92Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1114:22453:9032	Test_seq	414176	Test_seq	414453	+	-	UU	A00126:224:H7HMWDSX3:3:1114:22453:903297Test_seq41417660151M=414303278TGCCTCTTTGTCCACCTAGAAGCTTAGACAAGAAACCTGGAAGTCAGTCTTGATCCTTCTTCGTCCATCACCCCCACATCCAGTCGGTTATCAAAATACTTCTCAATTACTTCAACCTCTTCGCATCTCCACTGACAGCATTCTCCCCTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF,FF:FFFFF::FF:FF,FFFFFFFFFFF:F:FFFF,FFFFFFFFFFFFFF,,FF,FFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:22453:9032145Test_seq41430360151M=414176-278TCCACTGACAGCATTCTCCCCTCAATCACCATCGACTCGCATTAAGCCCCATTTAGTTCCATTTCCCTTTCAATCCATCTCTTCACAGCAGCCAGGGTGATTTAAAAAAATATATAAATTACAGCACATCACTCTGGTGTGTGGCCCACTGFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:27959:9392	Test_seq	171239	Test_seq	171657	+	-	UU	A00126:224:H7HMWDSX3:3:1114:27959:939297Test_seq17123960151M=171507419GTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGATTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGF,,FF:FFFFFFF,:FF:FF:FFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFF,F,FFFFFF,FFFFFFFFFFFFF,FFFFFFFFF:FF:FFFFFFFF:FFF,FFFF:FFFFFFFFFFFFF:FFFFF,,:,:FFFFFFFFFF,FFFFFFFFNM:i:1MD:Z:63G87MC:Z:151MAS:i:146XS:i:65Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:27959:9392145Test_seq17150760151M=171239-419TTTTTTCAGCTATGTTGCAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGGTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:17G46C11T74MC:Z:151MAS:i:136XS:i:47Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:13259:9518	Test_seq	305947	Test_seq	306239	+	-	UU	A00126:224:H7HMWDSX3:3:1114:13259:9518161Test_seq30594760151M=306104293GAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATACTAAAGAGGCAAAAGTCATGGAAACCACTGGAGGGCCATTATCTTTCCTCATCTCCCCCATGTTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:F:FFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:136M15SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:13259:951881Test_seq30610460136M15S=305947-293TCATCACAAAGGTCTGCGGTTGGTAAAGAGAGGAACTGGATGAAAGGGTTTTGATGGGTTGTTCAAATCTACCACCAAGGCTGTAAACAACTCCCTGAATGAAAGCATCATACTTACTCTTCTTCTAGCCTGGATTTAAAAAAGGGGGGAAFFF:FFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFF,FFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFF,FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:0Yt:Z:UU
@@ -574,7 +574,7 @@
 A00126:224:H7HMWDSX3:3:1115:1967:25974	Test_seq	167150	Test_seq	167599	+	-	UU	A00126:224:H7HMWDSX3:3:1115:1967:2597497Test_seq16715018151M=167449450TTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTAGCTGCCTGAATATCTTCTTTAGCGAAATGTGTGTTCATGTCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTCACAGAATFFFFFFFFFFFFFF:F,,FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFF:FFF,FFF:FF,FFFFFFFFFFF,FFFFFFFFF,F,,FFFF:::FFF:FFFF,FFFF,F:FFFF:F,,FFFF,FFFFFFFFF:FF:F:FFF:FNM:i:6MD:Z:28A23G22T15A1T49G7MC:Z:151MAS:i:121XS:i:111XA:Z:Test_seq,-213734,151M,8;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:1967:25974145Test_seq16744960151M=167150-450TCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTG:FFF:FFF,:FFFFF:FFFFF:FFFFF,FFFFF:FFFFFF:FFFF:FFFF:FFF,FFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFF,F,FFFFF:::FFFFFFFF:F:F,FFFFFFF:FF:FFFFFFFFFFFF:FFFFFFFFFF,NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:101Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1115:7789:25974	!	0	Test_seq	167947	-	-	NR	A00126:224:H7HMWDSX3:3:1115:7789:25974161Test_seq1675683432S119M=167797380CATATTTATGCCTATTACCAAGAAAGGTGATCGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGCTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:76T42MC:Z:151MAS:i:114XS:i:99XA:Z:Test_seq,-213352,108M2I9M32S,4;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1115:7789:2597481Test_seq16779760151M=167568-380CAGGCTGTTTTGACTACTGTAGCTGTATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:21C129MC:Z:32S119MAS:i:146XS:i:113Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1115:18032:26678	Test_seq	170112	Test_seq	170470	+	-	UU	A00126:224:H7HMWDSX3:3:1115:18032:26678161Test_seq17011260151M=170320359TGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFF:F,:FFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFF:FFFFFFFFF:FF:FF:FFF::FFFFFFFFFFF,FFF:FFNM:i:3MD:Z:3G20A59A66MC:Z:151MAS:i:137XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:18032:2667881Test_seq17032060151M=170112-359CTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:56C94MC:Z:151MAS:i:146XS:i:48Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1115:6686:27383	Test_seq	166310	Test_seq	166674	+	-	RU	A00126:224:H7HMWDSX3:3:1115:6686:2738397Test_seq16631060151M=166550365CCCAGGCGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:F,FF,FNM:i:1MD:Z:6T144MC:Z:26S125MAS:i:146XS:i:27Yt:Z:RU	A00126:224:H7HMWDSX3:3:1115:6686:27383145Test_seq1665506026S125M=166310-365TTATCCCGGAAACCTCTTTGCTTTTAGATCAATAAAAAACCCTCTCCCTCCCTTCCTCCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTACTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCFFFFFFFFFFFFFFF::,FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFF:FFFFFFNM:i:3MD:Z:27C50T9C36MC:Z:151MAS:i:110XS:i:63SA:Z:Test_seq,166445,-,27M124S,48,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1115:6686:273832193Test_seq1664454827M124H=166310-162TTATCCCGGAAACCTCTTTGCTTTTAGFFFFFFFFFFFFFFF::,FFFFFFFFFNM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,166550,-,26S125M,60,3;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1115:6686:27383	Test_seq	166310	Test_seq	166674	+	-	RU	A00126:224:H7HMWDSX3:3:1115:6686:2738397Test_seq16631060151M=166550365CCCAGGCGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:F,FF,FNM:i:1MD:Z:6T144MC:Z:26S125MAS:i:146XS:i:27Yt:Z:RU	A00126:224:H7HMWDSX3:3:1115:6686:273832193Test_seq1664454827M124H=166310-162TTATCCCGGAAACCTCTTTGCTTTTAGFFFFFFFFFFFFFFF::,FFFFFFFFFNM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,166550,-,26S125M,60,3;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1115:6686:27383145Test_seq1665506026S125M=166310-365TTATCCCGGAAACCTCTTTGCTTTTAGATCAATAAAAAACCCTCTCCCTCCCTTCCTCCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTACTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCFFFFFFFFFFFFFFF::,FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFF:FFFFFFNM:i:3MD:Z:27C50T9C36MC:Z:151MAS:i:110XS:i:63SA:Z:Test_seq,166445,-,27M124S,48,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1115:27516:27915	Test_seq	169316	Test_seq	169778	+	-	UU	A00126:224:H7HMWDSX3:3:1115:27516:27915161Test_seq16931660151M=169628463TAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATGTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,F:FFFFF,FFFF,FFFF::FFFFFF,FFF,FFNM:i:1MD:Z:37T113MC:Z:151MAS:i:146XS:i:107Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:27516:2791581Test_seq16962860151M=169316-463CGTTGCATTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATCTCTAGTTTTATTGCCTTGTGGTCTGAFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:8MD:Z:0T5T5A22G83C4T5C2C17MC:Z:151MAS:i:115XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1115:31584:28040	!	0	Test_seq	167925	-	+	NR	A00126:224:H7HMWDSX3:3:1115:31584:2804081Test_seq16821860128M23S=167925-421TCTTTTTGTTGATGTAGAGGAATCCAGGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTACTTTCAGTAGTTCTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCAATCATTTCTTCATTTGCTC:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:26A26T27G11T34MC:Z:151MAS:i:108XS:i:31Yt:Z:NR	A00126:224:H7HMWDSX3:3:1115:31584:28040161Test_seq16792560151M=168218421AGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF,FFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:135C15MC:Z:128M23SAS:i:146XS:i:73Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1115:15944:29731	Test_seq	166867	Test_seq	167319	+	-	UU	A00126:224:H7HMWDSX3:3:1115:15944:2973197Test_seq16686738151M=167169453GCTGCAATAAACATGGGTGTGCATATATCTGTTTGTATGAAGGCTCTTGTATCTCTAGGGTCTATTCCTAGGAGTGGGATTTCTGGGTTGTATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATGGATTTCCAAAGTGGTTGTAFFFFF,,FFFFFF:FFF:FFFFF:FFFFFFFF,FFF:FFF:FFF:F::FFFFFFF::FFF,,::FFFFFFFFFFFFFF:,::F:FFF,FFF,,FF,,FFFFF,FFFFFFFFFFF,FFFFFFF,F:FF:FFFFFFF:FFF::FFFFFFFFF,NM:i:5MD:Z:26G9G13T10A8T80MC:Z:151MAS:i:126XS:i:106XA:Z:Test_seq,-214017,151M,9;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:15944:29731145Test_seq16716952151M=166867-453TAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATC:FF,FF,F:FFF:FFFF,FFFFF,FF:FF:FFFFFFFFFF::FF:FFF:FFF:F,FFFFFF,F:FFFFFFF:FFFFFFFFFFFFF,FFFFFF:FFFF:FFF,FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFF:,FFFFFFFF:FFFFFFFNM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213715,151M,6;Yt:Z:UU
@@ -590,7 +590,7 @@
 A00126:224:H7HMWDSX3:3:1116:21097:1329	Test_seq	167966	Test_seq	212718	+	+	UU	A00126:224:H7HMWDSX3:3:1116:21097:132965Test_seq16796621151M=21271844753TTTGGATCGGAATTGCATTGTATGTATAGATGGCTTTTGGTAGAATAGACATTTTGACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTACTAGAGCTTTGTAGTTTTCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:11MD:Z:7T4G3G2A35T29G8C34G0C4T3A11MC:Z:151MAS:i:96XS:i:83XA:Z:Test_seq,-212922,151M,14;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:21097:1329129Test_seq21271831151M=167966-44753TCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCCCCCAFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:108A42MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,-168170,151M,4;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:9525:1548	Test_seq	165792	Test_seq	166199	+	-	UU	A00126:224:H7HMWDSX3:3:1116:9525:1548161Test_seq16579260110M2D41M=166049408CCGTCTTCCCTCTCTCTCCATCTTCCCATCCCTCCTCCAGTCAAGAGTTGCCAACACACTCTCCCGTGTCCACCTGATTTAATTAGCTCACTCTTCCTCAGCATCTCTCTCCCCCCCACTGACCAGTCCTTTTCATGCCTGATGAGTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:12MD:Z:2A5T9T13C31A10A8G11A13^CC7G19T13MC:Z:151MAS:i:95XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:9525:154881Test_seq16604960151M=165792-408ATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTACTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:53G97MC:Z:110M2D41MAS:i:146XS:i:50Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:28917:2190	Test_seq	74270	Test_seq	74579	+	-	UU	A00126:224:H7HMWDSX3:3:1116:28917:219097Test_seq7427060151M=74429310TGGGAGTCCAGGGAAGCCTTCTGGAGACCAAATCTGTTACCATCCGACTCATAGTGACCCTATAGGACAGAATAGAACTCCCCCATAGGGTTTCCAAGGCTGTAAACCTTTACAGAAGCAGATTGCCATGTCTTTCTCCCACGAAGCAGCTFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:41Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:28917:2190145Test_seq7442960151M=74270-310CGAACCGCGGACCTTTCAGTCAGCAGCCGAGTGCTTCACCACCGTACCACCAGGGCTCCCCTCCTGCGGAAAGTGATGCATAAACTGAAATCTGACCAATACATAGACTCAGCCCCCAGGATAAGAGGAACAAGAGCAGGAGTGTTCTAGGFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1116:3495:3724	Test_seq	335700	Test_seq	436771	-	-	UR	A00126:224:H7HMWDSX3:3:1116:3495:3724177Test_seq33566960119S32M=436621101072AAACTGGTGTTCAAATGTGTTTTACCTTTTGGAAGAAAAATATGATTTTCTAAAGGAGGAAAATAAGAAAAAGTATAGATTTTTCATAGTTTCACTTTTCACAATGTGGAAAAAAGATCGATCTATCCTGAAGTACCTTAAGGTTAGTGGAFF:FF:FFFFF,FFFFFFFFFFFFF:FFFFF,FFFF,FF:FFFF,FFF:FFF:FF:F,FFF:FFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,436401,+,32S119M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1116:3495:37242209Test_seq4364016032H119M=436621371GATCTTTTTTCCACATTGTGAAAAGTGAAACTATGAAAAATCTATACTTTTTCTTATTTTCCTCCTTTAGAAAATCATATTTTTCTTCCAAAAGGTAAAACACATTTGAACACCAGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:FFF,F:FF:FFF:FFF,FFFF:FF,FFFF,FFFFF:FFFFFFFFFFFFF,FFFFF:FF:FFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,335669,-,119S32M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1116:3495:3724113Test_seq43662160151M=335669-101072AAAAATGCCACGTTGGATTAACCTTGACTCAACTGGACATGTAGATACCCCATGTGGATGGTTAGAGGGGTGGCAAGGTCCATCCTCCCCAAAGAGACTGTATCCCTACCTGCCCGAGGACCTGAAGTGGTTACGTGTCCAACCAGTTGCCFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:151MC:Z:119S32MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1116:3495:3724	Test_seq	335700	Test_seq	436771	-	-	UR	A00126:224:H7HMWDSX3:3:1116:3495:37242209Test_seq4364016032H119M=436621371GATCTTTTTTCCACATTGTGAAAAGTGAAACTATGAAAAATCTATACTTTTTCTTATTTTCCTCCTTTAGAAAATCATATTTTTCTTCCAAAAGGTAAAACACATTTGAACACCAGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:FFF,F:FF:FFF:FFF,FFFF:FF,FFFF,FFFFF:FFFFFFFFFFFFF,FFFFF:FF:FFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,335669,-,119S32M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1116:3495:3724177Test_seq33566960119S32M=436621101072AAACTGGTGTTCAAATGTGTTTTACCTTTTGGAAGAAAAATATGATTTTCTAAAGGAGGAAAATAAGAAAAAGTATAGATTTTTCATAGTTTCACTTTTCACAATGTGGAAAAAAGATCGATCTATCCTGAAGTACCTTAAGGTTAGTGGAFF:FF:FFFFF,FFFFFFFFFFFFF:FFFFF,FFFF,FF:FFFF,FFF:FFF:FF:F,FFF:FFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,436401,+,32S119M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1116:3495:3724113Test_seq43662160151M=335669-101072AAAAATGCCACGTTGGATTAACCTTGACTCAACTGGACATGTAGATACCCCATGTGGATGGTTAGAGGGGTGGCAAGGTCCATCCTCCCCAAAGAGACTGTATCCCTACCTGCCCGAGGACCTGAAGTGGTTACGTGTCCAACCAGTTGCCFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:151MC:Z:119S32MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1116:1886:5885	Test_seq	133235	Test_seq	133657	+	-	UU	A00126:224:H7HMWDSX3:3:1116:1886:588597Test_seq13323560151M=133507423AATCATGGTCCAGCCAAGTTGGCACATAAGGTTAACAATCATAGCCAGCGTTACTCTTGCCTTGCACCTCGGTGTTCGTTACTGCCAGATGTACGTCGTTAATGTGCGTAACAGTCAGATACGAGATTTTTTACTATTGTCATGAACGTAAFFFFFFF:FFFF,FFFFFFF,FFFFF:FF,FFFF::,FFFFFFFFFF,F:FFFFFFFFF,FFFFFF,FFF,FFFFFF,FFFFF,FFFFFFFFFF:FFFFFFFFFF:::FFF,FFF:F,FFFF,F:FFFFFF:F,FFFF,F,FF::F,FFFFNM:i:1MD:Z:36C114MC:Z:151MAS:i:146XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:1886:5885145Test_seq13350760151M=133235-423GGGGTTTCACTGTCTGGTTTTTAGAAGCAGCATGCCAGGTCTCTGTTCCCTAGTCCACCTTAGTCTGGACACTCCGCTGAAACCTGTCCAGTGTCATAGCGACACACAGGCCTACACTGAGAGACAAGTGGTGGATGAATTTGAAGTACGT:F::FFFFFFFF,FF,FFF,,F:FFF,FFFFFF,FFF,:F:F::FFFF,FFF:F,:FFFFFFFF:,,F,:FF:FFFFFFFFFFFFFF:FFFF:F:FFFFF::FFFFFFFFF,F,FFFFFFFFFF,FFFFFFFFF,FF,F:FFFFFFFFF,FNM:i:5MD:Z:12G29T70C20C2C13MC:Z:151MAS:i:126XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:5041:6715	Test_seq	168462	Test_seq	168810	+	-	UU	A00126:224:H7HMWDSX3:3:1116:5041:671597Test_seq16846260151M=168674349GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFF::F:F:F,FFFFFFFFFFFF:F:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFF:FFFFFF:FF:FFFF,FFFFFFFF:FFF,F:,FFFFF:FFFNM:i:1MD:Z:33T117MC:Z:137M14SAS:i:146XS:i:91Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:5041:6715145Test_seq16867460137M14S=168462-349TCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTCTTTTGATATTGAACCAGCCTTGCATACCTGATATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTGATCTCCGGGACTTF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:F:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:42T10T2C7A19G52MC:Z:151MAS:i:112XS:i:30Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:31720:7545	Test_seq	303522	Test_seq	305328	+	+	RU	A00126:224:H7HMWDSX3:3:1116:31720:7545129Test_seq30352260151M=3053281807ATCTTTTATTTGGGAGAAACAAGTCAGAATTAATTCCTGTTGATTGCACTGAATTATAAATGATGAAGAATCAGAAATCAGCTACTGGCCCACAGGCATGTTGCAGAAACTCAATAAATGTTAGCCATCATCTCCTTTTTCATCATCATAAFFFFFFF:FFFF:F:FFFFFFFFFF:FFF:FFFF:FFFF,FFFFFFFFF,FFFFF::FFF:FFFFFFFFFFFFFFF,F:FFFFFFFFFF:FF:FF:FFFF,FFF:FFF:FFFFFF:FFFFFF,F:F,FFFFF:FFF:FFF:FFFF,FFF:FNM:i:0MD:Z:151MC:Z:20M131SAS:i:151XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1116:31720:754565Test_seq305328620M131S=303522-1807CCATTGCTATCAAGTGGATTGATCACTCCCTGGAGAAGGACATCATGCTTGGTAAAGTAGAGGGCCAACATGGATTGACACAGTGGCTGCAACAATGGGCTCAAGCATAACAACAACTGTGAGGATGGCGCAGGGCTGGGCAGTGTTTCATFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFF:FF:FFF:FFFFF:F:FFFFFFFFF,F::FFFFFF:FFFFFFFFFFFFFFF,F:FFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,303836,-,131M20S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1116:31720:75452129Test_seq30383660131M20H=303522-445ATGAAACACTGCCCAGCCCTGCGCCATCCTCACAGTTGTTGTTATGCTTGAGCCCATTGTTGCAGCCACTGTGTCAATCCATGTTGGCCCTCTACTTTACCAAGCATGATGTCCTTCTCCAGGGAGTGATCFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:F,FFFFFFFFFFFFFFF:FFFFFF::F,FFFFFFFFF:F:FFFFF:FFF:FF:FFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:43SA:Z:Test_seq,305328,+,20M131S,6,0;Yt:Z:RU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_parsed_pairs_sam_assemblyname.pairs
--- a/test-data/output_parsed_pairs_sam_assemblyname.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_parsed_pairs_sam_assemblyname.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -4,13 +4,13 @@
 #chromsize: Test_seq 450060
 #samheader: @SQ	SN:Test_seq	LN:450060
 #samheader: @PG	ID:bwa-mem2	PN:bwa-mem2	VN:2.2.1	CL:bwa-mem2 mem -5SP -T0 -t 8 test test_hic_reads_1.fq test_hic_reads_2.fq
-#samheader: @PG	ID:pairtools_parse	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpqgxtfoa0/files/1/0/c/dataset_10cd8bd7-2817-493f-9564-e4ab0fd42e68.dat -c /tmp/tmpqgxtfoa0/files/a/b/0/dataset_ab072667-6807-4c7f-9c2f-4a8de9dd1caa.dat --assembly test_assembly -o /tmp/tmpqgxtfoa0/job_working_directory/000/28/outputs/dataset_a8df527f-2db8-468b-a5eb-bd15b7e24367.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-mem2	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/5/2/e/dataset_52effa56-a42c-41a9-802e-401d7a5813de.dat -c /tmp/tmpchj83off/files/8/d/d/dataset_8dd0a91e-ecbd-4f98-86cd-2510709cac82.dat --assembly test_assembly -o /tmp/tmpchj83off/job_working_directory/000/28/outputs/dataset_0a87c13d-5811-4e81-9626-bb8665d07df4.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-mem2	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 A00126:224:H7HMWDSX3:3:1101:4779:3098	Test_seq	166211	Test_seq	214901	-	-	UU	A00126:224:H7HMWDSX3:3:1101:4779:3098113Test_seq1661344378M=21482448691GGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFNM:i:0MD:Z:78MC:Z:78MAS:i:78XS:i:63XA:Z:Test_seq,+214829,5S73M,2;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:4779:3098177Test_seq214824578M=166134-48691AAGAGCCCAAGAGACAGAAAGGGCCACATGAACCACAGACCTACATCATCCTGAGACCAGAAGAACTAGTTGGTGCCCFFFF,FFFFFFFFFFFFF,FFFFFFFFFFFF:FF:,FFFFFFF:FF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:3MD:Z:1T33G33A8MC:Z:78MAS:i:66XS:i:63XA:Z:Test_seq,+166134,78M,3;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:2908:3239	Test_seq	167331	Test_seq	167696	+	-	UU	A00126:224:H7HMWDSX3:3:1101:2908:3239161Test_seq16733123151M=167546366TGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFF:FFFFF:FFFF:FFFFFFFFFFFFFFF:FF,,FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFF:FF,FFNM:i:1MD:Z:37A113MC:Z:151MAS:i:146XS:i:135XA:Z:Test_seq,-213553,151M,4;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:2908:323981Test_seq16754644151M=167331-366TAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTACAAATGGATATCCAGGTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACFF,F,F:FFFFFFFFF,FFF:FFFFFF:FFFF:FFFFFFFFFFF:FF::F,FFFFFFFFF:,F,FFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFF::FFFFFFFFFFFFF,FFFFFFF:FF:F,:FFFFFFFFFF,F,F:FFFFFFFFFFNM:i:3MD:Z:82G15T30C21MC:Z:151MAS:i:136XS:i:114XA:Z:Test_seq,+213342,118M2I23M2I6M,8;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:27208:4492	Test_seq	194620	Test_seq	194909	+	-	UU	A00126:224:H7HMWDSX3:3:1101:27208:449297Test_seq19462060151M=194778290GCTGTGCAAACCACTGCTCCAGGCTATCTCAGCACTGAGCCAGAGACAAGGGCCAGTTCAGTCACCTGGAAAATCAGGCCAGCATCTCTGAAAAATAGGTGCCTCAGTGTTCACAGAAAAGGGCCATGTATTGAAGTAGGTCAGAGGAAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,FF:::FFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:27208:4492145Test_seq19477860132M19S=194620-290CACTCACCAGATTCAGCCCAGGACCAGAGGCTGCCGAGGGCAGGTGTGGGACGAAGTCAAGATGAAGTCAGTTCATTGTGTGGTTTTCGGTACCAGGCTAGACCTAGATTTGAACCCCAAGTCTGCTACTTAGATCATTCATTCAATGTGTFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:9344:5306	Test_seq	449503	Test_seq	449562	+	-	UU	A00126:224:H7HMWDSX3:3:1101:9344:530697Test_seq449503760M=44950360GTTCGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTATGAGTCAGTGT,FFFF,FFF:FF,FF:FF:FF:F:,F::,FF:,FFFFFFFF::FF,,:FFF,FFF::,F,NM:i:4MD:Z:3T44G2C5G2MC:Z:60MAS:i:44XS:i:40XA:Z:Test_seq,+16926,60M,5;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:9344:5306145Test_seq4495031260M=449503-60GTTTGGACAGAACAAGGGGATACTGATTGGTTTAAAGTCAGGAAAGGTGTGCGCCAGGGTFFFFFFFFF:F::FF,FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:F:FFFFNM:i:1MD:Z:53T6MC:Z:60MAS:i:55XS:i:50XA:Z:Test_seq,-16926,60M,2;Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1101:17607:5212	Test_seq	83425	Test_seq	93852	-	+	UR	A00126:224:H7HMWDSX3:3:1101:17607:521281Test_seq8339760122S29M=9385210428GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATCAATCCAGCTGATTTTTGTGTGTTTACCTT:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,94155,-,122M29S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1101:17607:52122129Test_seq9415560122M29H=93852-425GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATC:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0SA:Z:Test_seq,83397,-,122S29M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1101:17607:5212161Test_seq9385260151M=83397-10428AAACATGGAACGGCAAAAAATGTGGCACCGACCCGGCTATATGAAGGACACTTGTTTACAGTGTGAGAGCTGAAACAAGAAGGCAGAGTGCGGCTTTGTTTGACCTCTGTTGGAAACGCGTGCATCGGGTGATTCAAAATTTCCACCACTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:122S29MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1101:17607:5212	Test_seq	83425	Test_seq	93852	-	+	UR	A00126:224:H7HMWDSX3:3:1101:17607:52122129Test_seq9415560122M29H=93852-425GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATC:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:0SA:Z:Test_seq,83397,-,122S29M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1101:17607:521281Test_seq8339760122S29M=9385210428GCAATGAAGCAGCAGTTTTTGTAGTGAAGGGAAATGTCCTGATAACATTAAGTAAGAAATGAACTACAGGCCAGGGCATTGGATTGATTTAGTGTGGAGTTTCTCCAGCAATTTGTCAGATCAATCCAGCTGATTTTTGTGTGTTTACCTT:FF:FFF:FFFF,FFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,94155,-,122M29S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1101:17607:5212161Test_seq9385260151M=83397-10428AAACATGGAACGGCAAAAAATGTGGCACCGACCCGGCTATATGAAGGACACTTGTTTACAGTGTGAGAGCTGAAACAAGAAGGCAGAGTGCGGCTTTGTTTGACCTCTGTTGGAAACGCGTGCATCGGGTGATTCAAAATTTCCACCACTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:122S29MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1101:17942:7263	Test_seq	168528	Test_seq	168867	+	-	UU	A00126:224:H7HMWDSX3:3:1101:17942:726397Test_seq16852860151M=168717340ATTTAGAGTGATGTTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTGAGAGTTTTTATCATAAATGGGTGCTGGACTTTGTCAAATGCCTTTTCTGCATCAATTGATAAGATCATGFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,FFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFF:FFFF,FFFFFFFFF:FFFFF,::FFFF::F:FFFNM:i:5MD:Z:12A68A23T2A26T15MC:Z:151MAS:i:126XS:i:91Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:17942:7263145Test_seq16871760151M=168528-340ATTAATGGTTTTTCTGATATTAAACCAACCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:27G123MC:Z:151MAS:i:146XS:i:91Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:5683:8108	!	0	Test_seq	170834	-	+	MR	A00126:224:H7HMWDSX3:3:1101:5683:810865Test_seq137032028M123S=17083433803GATTTTTATTTTCTTTATGTTGAGGTGTGATCGATCCAAAAAGAAAAACACCAAGACATATTATCATCAAACTTGCCAAAACCAAAGACAAACAGAAAATTTTAAAAGCAGCCAGGGAGAAAAGAAAGGTTTCCTTCAAGGGAGAATCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF:FFFFFNM:i:1MD:Z:7G20MC:Z:151MAS:i:23XS:i:23SA:Z:Test_seq,171170,-,119M32S,60,0;XA:Z:Test_seq,+333108,28M123S,1;Test_seq,-298750,123S26M2S,1;Test_seq,-279319,123S28M,2;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1101:5683:81082129Test_seq17117060119M32H=170834-455ATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFF:FFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,137032,+,28M123S,0,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1101:5683:8108129Test_seq17083460151M=137032-33803ATCTTACTTTGTTTGTTTTTGTGCTTTCCCTATTTGAGTTGCGTTGATATCAGGACGTTCTGTTTTGTGACCTTGTATTGTGCTGGTACCTGATATTATTGGTCATCAGGCCAAACAATCTCCTTAAGCATTTCTTGCAGTCTTGGTTTAGFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:28M123SAS:i:151XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1101:3947:9361	!	0	Test_seq	171174	-	+	NR	A00126:224:H7HMWDSX3:3:1101:3947:9361145Test_seq17134260129M22S=171174-297TGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTATAAAAATAAAATGCTTGCTTACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFNM:i:2MD:Z:58G15T54MC:Z:151MAS:i:119XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1101:3947:936197Test_seq17117460151M=171342297ATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGAFFFFFFFFFFFFFFFF,,:FF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFF:F:FFFFFFFF::FFFFFF:FFFFFFFF:FFFFF,FFFFFFFFFF,FFFFFFF:FFF,FNM:i:0MD:Z:151MC:Z:129M22SAS:i:151XS:i:37Yt:Z:NR
@@ -46,12 +46,12 @@
 A00126:224:H7HMWDSX3:3:1101:13042:33630	Test_seq	166363	Test_seq	166687	+	-	UU	A00126:224:H7HMWDSX3:3:1101:13042:3363097Test_seq16636360151M=166544325TTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF,F:FFF:FF:,FFF,FFFF:F,:FFFFFFFF::FF:F,NM:i:0MD:Z:151MC:Z:5M3I18M4I121MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:13042:33630145Test_seq166544605M3I18M4I121M=166363-325CTGATTGATGATCAATAAAAAACCCTCTCCCTCCCTCCCTCCCTCCCTCCCCCCTTTGTAACCACATAAGTATGTGTTCTTCTCCGTTTTTTCTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCTTTTGCCTCTGACF:,FFFFFFFFFFFFF:FFFFFF:F,FFFFFFFF:FF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFFF:F:FFFFFF:,FFFFF,FFFFFNM:i:9MD:Z:59A34C49MC:Z:151MAS:i:119XS:i:62Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:29966:36072	Test_seq	170104	Test_seq	170357	+	-	UU	A00126:224:H7HMWDSX3:3:1101:29966:36072161Test_seq17010460151M=170207254TCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFF:F:FFFFFFFFFFFFFF:,:FFFF:F:FFFFFFFFFFNM:i:6MD:Z:3C7G20A32A25C0A58MC:Z:151MAS:i:122XS:i:49Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:29966:3607281Test_seq17020760151M=170104-254TAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFF:F:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFNM:i:1MD:Z:55A95MC:Z:151MAS:i:146XS:i:88Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1101:3595:36620	Test_seq	167567	Test_seq	167976	+	-	UU	A00126:224:H7HMWDSX3:3:1101:3595:36620161Test_seq1675673015S136M=167826410ACCAACCAGCTAGACTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATTGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGATAAAAAGACTATCATTTCCCCAAATGACTGACACTGGGCCFFFFFFFF,,FF:FFFFFF,FF:FFFFF:FFFFF:FFFFF:FFFFFFFFFF:FFFF,FF:,,FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:,:F,FFFF:FFFFFFF:F:FFFFF,FF,F:FF,:FFFFFFFF::NM:i:4MD:Z:41G53T23T13T2MC:Z:151MAS:i:118XS:i:103XA:Z:Test_seq,-213336,124M2I10M15S,7;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1101:3595:3662081Test_seq16782660151M=167567-410ATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCACATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAFFFFFFFFFFFFF::FFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF::F,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFF,FFFFFFF:FF,FF:,FFFFFFFFFFFFFFFNM:i:1MD:Z:90T60MC:Z:15S136MAS:i:146XS:i:87Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1102:11306:1908	Test_seq	186004	Test_seq	256018	-	-	RU	A00126:224:H7HMWDSX3:3:1102:11306:1908177Test_seq18585460151M=25599370015ATGTGCCAGGTGCTGTTTTATGCTTTCATCTTCACTACTTCAGTCAGTCCTCACTGCAAACCAGTGAGGCAGATAGTATTATTCCCACTTTACAGTTGAAGAGACTGAGGCTCTATGAAATTAAGAAAATGTCCCCAGCCACAGGGCTAGTFFFFF:::F,F:FF:F:FFFF::FFF,FF,F,F:FFF:F:,F:FFFFFF:FFFFFF:FFF,:F::FFFF:FFFF:FFFFFFFFFFFFFFFFF:F:FF,FFFFFFF:FFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:31G119MC:Z:125S26MAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:11306:1908113Test_seq25599342125S26M=185854-70015AGTAGTATTTAGCTATTCTTTCCCTCATATTTTCCAACAATGTCATTTTGCTCTAATTTTTCAAAAGTGTCTTCTGCTTTATTTTCCAGCTTATTGATTTTTTTATATTAGTAATAATTTTGATCGATCTAAATGCCCAATCAACCAACTT,,FF,::F:F:,:FFF:,F:FF,FF,:F,FFFFF,F,F,FFFFFFFF:F,FF:F:F,FFFFFF::FFF,F,FFFFFFFFFFFFFF,,FFF:FFFFF,FFFFF:FFFFFFFFFFFF,FFFFFFFFF:FFFFF,F,F:FFFFFFFFFFFFFFFNM:i:0MD:Z:26MC:Z:151MAS:i:26XS:i:0SA:Z:Test_seq,185684,+,26S125M,60,3;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:11306:19082145Test_seq1856846026H125M=185854321GATCAAAATTATTACTAATATAAAAAAATCAATAAGCTGGAAAATAAAGCAGAAGACACTTTTGAAAAATTAGAGCAAAATGACATTGTTGGAAAATATGAGGGAAAGAATAGCTAAATACTACTFFFFFFFFF,FFFFFFFFFFFF:FFFFF,FFFFF:FFF,,FFFFFFFFFFFFFF,F,FFF::FFFFFF,F:F:FF,F:FFFFFFFF,F,F,FFFFF,F:,FF,FF:F,:FFF:,:F:F::,FF,,NM:i:3MD:Z:68T17G1G36MC:Z:151MAS:i:110XS:i:0SA:Z:Test_seq,255993,-,125S26M,42,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:11306:1908	Test_seq	186004	Test_seq	256018	-	-	RU	A00126:224:H7HMWDSX3:3:1102:11306:1908177Test_seq18585460151M=25599370015ATGTGCCAGGTGCTGTTTTATGCTTTCATCTTCACTACTTCAGTCAGTCCTCACTGCAAACCAGTGAGGCAGATAGTATTATTCCCACTTTACAGTTGAAGAGACTGAGGCTCTATGAAATTAAGAAAATGTCCCCAGCCACAGGGCTAGTFFFFF:::F,F:FF:F:FFFF::FFF,FF,F,F:FFF:F:,F:FFFFFF:FFFFFF:FFF,:F::FFFF:FFFF:FFFFFFFFFFFFFFFFF:F:FF,FFFFFFF:FFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:31G119MC:Z:125S26MAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:11306:19082145Test_seq1856846026H125M=185854321GATCAAAATTATTACTAATATAAAAAAATCAATAAGCTGGAAAATAAAGCAGAAGACACTTTTGAAAAATTAGAGCAAAATGACATTGTTGGAAAATATGAGGGAAAGAATAGCTAAATACTACTFFFFFFFFF,FFFFFFFFFFFF:FFFFF,FFFFF:FFF,,FFFFFFFFFFFFFF,F,FFF::FFFFFF,F:F:FF,F:FFFFFFFF,F,F,FFFFF,F:,FF,FF:F,:FFF:,:F:F::,FF,,NM:i:3MD:Z:68T17G1G36MC:Z:151MAS:i:110XS:i:0SA:Z:Test_seq,255993,-,125S26M,42,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:11306:1908113Test_seq25599342125S26M=185854-70015AGTAGTATTTAGCTATTCTTTCCCTCATATTTTCCAACAATGTCATTTTGCTCTAATTTTTCAAAAGTGTCTTCTGCTTTATTTTCCAGCTTATTGATTTTTTTATATTAGTAATAATTTTGATCGATCTAAATGCCCAATCAACCAACTT,,FF,::F:F:,:FFF:,F:FF,FF,:F,FFFFF,F,F,FFFFFFFF:F,FF:F:F,FFFFFF::FFF,F,FFFFFFFFFFFFFF,,FFF:FFFFF,FFFFF:FFFFFFFFFFFF,FFFFFFFFF:FFFFF,F,F:FFFFFFFFFFFFFFFNM:i:0MD:Z:26MC:Z:151MAS:i:26XS:i:0SA:Z:Test_seq,185684,+,26S125M,60,3;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1102:7491:2785	!	0	Test_seq	168666	-	-	NR	A00126:224:H7HMWDSX3:3:1102:7491:278597Test_seq1682384324S127M=168516429CACTTGATGACCCAAAATCAGATTAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCATGTCATCTGCAAATAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:2MD:Z:33T74G18MC:Z:151MAS:i:117XS:i:97XA:Z:Test_seq,-212674,127M24S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1102:7491:2785145Test_seq16851660151M=168238-429CAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAGTTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:79A71MC:Z:24S127MAS:i:146XS:i:82Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1102:22987:3724	Test_seq	272454	Test_seq	288164	-	+	UR	A00126:224:H7HMWDSX3:3:1102:22987:3724145Test_seq27243130127S24M=28816415711CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTTAAAATGTACAGCCATCATGAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,288412,-,128M23S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1102:22987:37242193Test_seq28841260128M23H=288164-376CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0SA:Z:Test_seq,272431,-,127S24M,30,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1102:22987:372497Test_seq28816460151M=272431-15711TAAGGCATGGTAGAACCAGAGATGTCCCACTACACTGCTTTCCACCTGGGGACACTGGGTACGGGAAAGGTTCCCTTAGAGTTCTAGCTTTTCTTTTTTTGAAGAATTTATACAACAGGGAATTTTGAGAAGCCAGCTAAAATGCCCACGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1102:22987:3724	Test_seq	272454	Test_seq	288164	-	+	UR	A00126:224:H7HMWDSX3:3:1102:22987:37242193Test_seq28841260128M23H=288164-376CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:128MC:Z:151MAS:i:128XS:i:0SA:Z:Test_seq,272431,-,127S24M,30,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1102:22987:3724145Test_seq27243130127S24M=28816415711CACTATTTTCTCAACCTCAATGTCATTTGTCCTTAAATGACACCCACTTCCATACCACCGCTCATTCAGGTTTTCTTTTATCTGTGTATCCCTAAAGACAAACAAATCTCAATGACGTCTGTGACTTTTAAAATGTACAGCCATCATGAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,288412,-,128M23S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1102:22987:372497Test_seq28816460151M=272431-15711TAAGGCATGGTAGAACCAGAGATGTCCCACTACACTGCTTTCCACCTGGGGACACTGGGTACGGGAAAGGTTCCCTTAGAGTTCTAGCTTTTCTTTTTTTGAAGAATTTATACAACAGGGAATTTTGAGAAGCCAGCTAAAATGCCCACGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1102:11822:4335	Test_seq	166209	Test_seq	214876	-	-	UU	A00126:224:H7HMWDSX3:3:1102:11822:4335113Test_seq1661592651M=21482648668CAGGATAATGTAGGTCTCTGGTTCATGTCGCCCTTTCTGTCTCTTGGGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28G22MC:Z:51MAS:i:46XS:i:38XA:Z:Test_seq,+214826,51M,3;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:11822:4335177Test_seq2148262851M=166159-48668GAGCCCAAGAGACAGAAAGGGCCACATGAACCAGAGACCTACATCATCCTGFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFNM:i:0MD:Z:51MC:Z:51MAS:i:51XS:i:43XA:Z:Test_seq,+166159,51M,2;Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1102:31313:5556	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31313:555697Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31313:5556145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31313:55562193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RU
-A00126:224:H7HMWDSX3:3:1102:31394:5541	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31394:554197Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFF:F:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31394:5541145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFF,,FFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31394:55412193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:31313:5556	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31313:555697Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31313:55562193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31313:5556145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFF:FFFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1102:31394:5541	Test_seq	151016	Test_seq	222264	+	-	RU	A00126:224:H7HMWDSX3:3:1102:31394:554197Test_seq15101660151M=22223571249TCCCTCACTGTTCTTTATCGATGTGTAGTATTCCATTCTGTGAATATGCCATAATTTATTTATCCATTCATCTGTTGATGGGCATCTTGGTTGCTTCCAGCTTCTTGCTGTTATAAACAGTGCTGCAATAAACATGGGTGTGCATATATCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFF:FFFFFF:F:FFFNM:i:0MD:Z:151MC:Z:121S30MAS:i:151XS:i:22Yt:Z:RU	A00126:224:H7HMWDSX3:3:1102:31394:55412193Test_seq15130047121M30H=151016-405CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:101SA:Z:Test_seq,222235,-,121S30M,60,0;XA:Z:Test_seq,-48995,15M2I104M30S,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1102:31394:5541145Test_seq22223560121S30M=151016-71249CCACAAGCAGTGTATAAGTGTTCCAATCTCTCCACAGCCTCTCCAACATTTATTATTTTGTGTTTTTTGGATTAATGCCAGCCTTGTTGGAGTGAGATGAAATCTCATTGTAGTTTTGATCGATCCATTTTGAATTAGTTTTTGTGTATGCFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFF,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFF:FFFFF,,FFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,151300,-,121M30S,47,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1102:4218:5822	Test_seq	170181	Test_seq	170586	+	-	UU	A00126:224:H7HMWDSX3:3:1102:4218:5822161Test_seq17018160151M=170436406TTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATGTTTTTTTACTTCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFF:FF:F:FFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFF,F,:FFFFFFFF::FFFFFFF,:FFFF:FF,FFF,FFFFFFF::,F::F:F:FFF:FFFFFFFFF,F:,FFFFF:FF:,NM:i:4MD:Z:15A78A7C1A46MC:Z:151MAS:i:131XS:i:55Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:4218:582281Test_seq17043660151M=170181-406TAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTFFFFFFFFFFFFF::F:FFFF,FFFFFFFFF:FFFFF:FFFFFF,FFFFFFFF:FF:FFFF,FFF:FFFFF:FFFFFFFFFF:FFFFF,F,FFFF,F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FF,FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:25Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:18602:6652	Test_seq	171219	Test_seq	171678	+	-	UU	A00126:224:H7HMWDSX3:3:1102:18602:6652161Test_seq17121960151M=171528460AAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:NM:i:1MD:Z:141G9MC:Z:151MAS:i:146XS:i:79Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:18602:665281Test_seq17152860151M=171219-460TGTTTCCCTGGTCCTCCAGAAGTCCCAGCCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGTGTTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCCTGCAACTTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:2A25T26T16C6T59T8A2MC:Z:151MAS:i:120XS:i:53Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:15619:9721	Test_seq	170074	Test_seq	170377	+	-	UU	A00126:224:H7HMWDSX3:3:1102:15619:9721161Test_seq17007425151M=170227304AAAATTTGTTTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATGTAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAG,,:F::FFFFFFFFFF:FFFFFF,FFFFFFFF::FFFFFF::::::FFF:FF:F:FFFFFFFFFFFF:FFF:FF::FFFFFFFF:FFF:FFF,,FFFFFFF:FFF:FFFFFFFFF:FF:FFFFFFFFF:FFFF:FFFFFFFF:F,FFFFFFNM:i:7MD:Z:8A24C7G20A32A25C0A28MC:Z:151MAS:i:116XS:i:102XA:Z:Test_seq,-38508,147M4S,9;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:15619:972181Test_seq17022760151M=170074-304ATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGFF:FFFF::FF:FF,FFFF:FFF:F,F:FFFFF,FFF,FFF:F:FFFFFFFF:F,,FFF:FFF:FFFF:F:::FF,FFF::F,FFFFFF::F,FFFFFFFFFFFFFFF:F:FFFFFFFFFF:FFFFFFFFFFFF,FFFF:,:FFF:F,FFFNM:i:1MD:Z:35A115MC:Z:151MAS:i:146XS:i:78Yt:Z:UU
@@ -78,7 +78,7 @@
 A00126:224:H7HMWDSX3:3:1102:24379:34413	Test_seq	169290	Test_seq	169639	+	-	UU	A00126:224:H7HMWDSX3:3:1102:24379:3441397Test_seq16929060151M=169489350TATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:102Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:24379:34413145Test_seq16948960151M=169290-350ATTTGTTCAAGTTGTCAGGACAGTTCTCTGATTTTGGCTCTTTCTTCTTTTTGTATGTGTGCATTTATCGATAAAAATTGGCCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCTTTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:30C0C41T65T11MC:Z:151MAS:i:131XS:i:70Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1102:2031:34757	!	0	Test_seq	167704	-	-	NR	A00126:224:H7HMWDSX3:3:1102:2031:34757177Test_seq2135927127M24S=167554-46015CGATAACTGGTATCTCCTAAAAATCAAACACCTATGCTCATCTAAAGACTTCACCAAAAGAGTAAAAAGACCACCTACAGACTGTGAAAGAATTTTCAGCTATGACATCTCAGACCAGCGCCTGATCGATCTCAAACTTTTGCCTAGTAGAF,:FFF,F,,,F,F::FFFFFFF,FFFFFF,F,FF:,F:F,F,F:F,FFF:FFFFFFFF:FF:FFFFF::,:F::FF:F,F,F,,:FFF:FF:FF:,F,F,:F,FFFF,FFF,FFFFFFFF,FFFF,FFF,F,FFF,FFFF,F:FF::F:FNM:i:6MD:Z:0A9G1G64T6G26C15MC:Z:151MAS:i:101XS:i:96XA:Z:Test_seq,+167316,24S127M,7;Test_seq,+400737,24S114M13S,5;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1102:2031:34757113Test_seq16755431151M=21359246015ATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCTCCAATTGACTGACACTGGTCCTTFFF:FFFFFF,:FF:FF:,FFFF:FFFFFF,:,FFF:FFFFFFFFF::F:,FFFFFFFF:F,F:F,FFFF,:FFF:F:FFF:FFFFF:FFF:FF,,FF:FF:,FFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFF,FFF,FFFFF,FFNM:i:1MD:Z:127C23MC:Z:127M24SAS:i:146XS:i:131XA:Z:Test_seq,+213334,126M2I23M,4;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1102:12563:35650	Test_seq	346775	Test_seq	347269	+	-	UU	A00126:224:H7HMWDSX3:3:1102:12563:35650161Test_seq34677560151M=347119495GTTTCTTAGACATGGAAACCCTGGTGGTGTAGTGGTTAAGTGCTACGGGGCTGCTAACCAAGAGGTCGGCATTTCGAATCCACCAGGCACTCCTTGGAAACTCTATGGGGCAGTTCTACTCTGTCCTATAGGGTCGCTATGAGTCGGAATTFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFF:FFFF:F:FFFFFFFFF,FF:F,FF,FFF,FNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:77Yt:Z:UU	A00126:224:H7HMWDSX3:3:1102:12563:3565081Test_seq34711960151M=346775-495ATATAAACCAAAAAATAGATAAGATAGAAAGTATTAGGCTAAGTTATGGAGAGATTTTTGAGGAGTTTTTTTTTTTTAATTTTTATTGTGCTTGAAGTGAAAGTTTACAAATCAAGTCAGTCTCTCATAGAAAAATTTATATACACCTTGCFFF:FFFFF:FFFF:F::FF::FFFF,FF::FF:FF,,FF:F:FFFF:FF:FFFFFFFFFF:FF::FFFFFF::FFFFFFFFF,FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:59Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1103:19000:1892	!	0	Test_seq	169431	-	-	MR	A00126:224:H7HMWDSX3:3:1103:19000:1892177Test_seq4000600122S29M=169281-230658CAACTAAATAGACCCATAAAAAAAAACGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAGATCTTATAATAGTGGTCTTATACAGTAFFFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFF:FF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26A2MC:Z:151MAS:i:26XS:i:26SA:Z:Test_seq,168996,+,28S123M,60,2;XA:Z:Test_seq,-166639,122S29M,1;Test_seq,+214367,18M1I10M122S,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1103:19000:18922209Test_seq1689966028H123M=169281436TAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCGTTTTTTTTTATGGGTCTATTTAGTTGFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFF:FF:FFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFFFNM:i:2MD:Z:96T6G19MC:Z:151MAS:i:113XS:i:36SA:Z:Test_seq,400060,-,122S29M,0,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1103:19000:1892113Test_seq16928160151M=400060230658TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28T122MC:Z:122S29MAS:i:146XS:i:103Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1103:19000:1892	!	0	Test_seq	169431	-	-	MR	A00126:224:H7HMWDSX3:3:1103:19000:18922209Test_seq1689966028H123M=169281436TAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCGTCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCGTTTTTTTTTATGGGTCTATTTAGTTGFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFF:FF:FFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFFFNM:i:2MD:Z:96T6G19MC:Z:151MAS:i:113XS:i:36SA:Z:Test_seq,400060,-,122S29M,0,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1103:19000:1892177Test_seq4000600122S29M=169281-230658CAACTAAATAGACCCATAAAAAAAAACGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGACGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAGATCTTATAATAGTGGTCTTATACAGTAFFFFFFFFFFFFFFF:FFF,FFF:FFFFFFFFFFFFFF:FF:FFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:26A2MC:Z:151MAS:i:26XS:i:26SA:Z:Test_seq,168996,+,28S123M,60,2;XA:Z:Test_seq,-166639,122S29M,1;Test_seq,+214367,18M1I10M122S,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1103:19000:1892113Test_seq16928160151M=400060230658TATTCGGGTTATTTGTTTCCTTTCCTGTATTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:28T122MC:Z:122S29MAS:i:146XS:i:103Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1103:30544:2378	!	0	Test_seq	168612	-	-	MU	A00126:224:H7HMWDSX3:3:1103:30544:2378161Test_seq1682780151M=168462335CTCTGCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGTTAGCTTTACTTCCTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCGTAATTGTFFFF:,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,:FFF:,FNM:i:12MD:Z:4T53C0A6C0G0G18A2A25T7C18C6C0MC:Z:151MAS:i:95XS:i:95XA:Z:Test_seq,-212610,151M,12;Yt:Z:MU	A00126:224:H7HMWDSX3:3:1103:30544:237881Test_seq16846260151M=168278-335GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAFFFFFFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:91Yt:Z:MU
 A00126:224:H7HMWDSX3:3:1103:25880:3098	Test_seq	183557	Test_seq	184047	+	-	UU	A00126:224:H7HMWDSX3:3:1103:25880:3098161Test_seq18355760151M=183897491ATCCAGTTGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCACFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:30Yt:Z:UU	A00126:224:H7HMWDSX3:3:1103:25880:309881Test_seq18389760151M=183557-491TCAATTCTCCGGTCTTTCTTAGTCATTGTTCAGCTTTCAAATGCATATTATGCGATTGAAAACACCATGGTTTGGGTCAGGTGCCCCTTAGTCCTCAAAGTGACATCTTTGCTTTTTAACACTTTAAAGAGGTCTTTTGCAGCAGATTTACFFFF:FFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:64Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1103:26639:3818	Test_seq	41455	Test_seq	214212	+	-	UU	A00126:224:H7HMWDSX3:3:1103:26639:381897Test_seq414556151M=214062172758TGGGCAAAGGATATGAACAGGCACTTCACTAAAGAAGACATTCAGGTAGCTAACAGATACATGAGGAAATGCTCATGATCATTAGCCATCAGAGAAATGCAAATCAAAACTATGACGAGATACCATTTCACCCCAACAAGGCTGGCATTAA:FFF:F:FFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F:FFFFFFFFFFFFFFFF:FF:FFFF:FFFFFF::FFFFFF:FFFFFFF,F:FNM:i:13MD:Z:20A25C0G26T0C13T22C0A1T5T4C4T15C3MC:Z:151MAS:i:87XS:i:81XA:Z:Test_seq,-49064,151M,14;Test_seq,-151371,151M,15;Test_seq,+258709,151M,16;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1103:26639:3818145Test_seq21406260151M=41455-172758AAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGGAATATACCCTAGAGAAATAAGAGCCTTCACACAAACAGATATATGCACACCCATGTTTATTGCAGCTCTGTTTACAATAGCAAAAAGCTGGAAGCAACCAAGGTGTCCGTCF,FFFFFF:FFF,FFFFFFFFFF:FF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFF,FFFFF:FFFFF,FFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:148A2MC:Z:151MAS:i:148XS:i:116Yt:Z:UU
@@ -136,7 +136,7 @@
 A00126:224:H7HMWDSX3:3:1104:21658:7279	!	0	Test_seq	293960	-	-	NR	A00126:224:H7HMWDSX3:3:1104:21658:727997Test_seq2935986032S119M=293810363ACAAAAACATACTGTGAAAACGAGTCTAGAGTAATCCTAAACCTATACTTATGAATGTCATCCTGTCTGGAAATAGAGTTTTCTTTGTTATGTTAATGAGGTCATATCAGTGCCCTAAACCCAAGCTTTTGTGAGTTACAAGGAGAAGCATFFFFF::FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FF,FFFFFFFFFFFFF,FFFF::FFFFFFFFFFF,FFFF::FFFFFFFFFFFFFFFFFFF:,FF,FFF:FFFFFFFF,FFFF:F,FFFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:21658:7279145Test_seq29381060151M=293598-363GAAAAAGACCAGAATCCTCCTCCACTGTCAAAAAGGAGCCTTCCCCTGGAGCAGGAGCCCTGAATTAGGACTTCCAGCCTCCAGACCAGTGAGAAAATAAATTTCTCTTCTTTAAAGCCCCCACTTGCAGTATTTCTGTTACAGCAGCACTFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFF:FFFFFFF,FFFFFF::FFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFF,FFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:85A65MC:Z:32S119MAS:i:146XS:i:0Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1104:8169:7498	!	0	Test_seq	167708	-	-	NR	A00126:224:H7HMWDSX3:3:1104:8169:749897Test_seq167315827S124M=167558394GCCCTCCTGTGGCCAGAAAAATCTCTCAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF::FFFFF:,FFFFFFFFFFF,FFFFFFFFFFFFFF,FNM:i:2MD:Z:8A44A70MC:Z:151MAS:i:114XS:i:109XA:Z:Test_seq,-213596,124M27S,3;Test_seq,+400736,27S124M,5;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:8169:7498145Test_seq16755831151M=167315-394TTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCTTTTCCCCAATTGACTGACACTGGTCCTTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:118A32MC:Z:27S124MAS:i:146XS:i:131XA:Z:Test_seq,+213330,130M2I19M,4;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1104:5791:7921	!	0	Test_seq	171294	-	-	NR	A00126:224:H7HMWDSX3:3:1104:5791:7921161Test_seq1710696024S127M=171144226ACTATTCTAGCTATCAGCAAGGATTGATCTTTGGTTGGCAGTTTTTCTCGTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTFFFFFFFFFFFFFFFFFFFFFFF:FFFF::FFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:5C16A2C101MC:Z:151MAS:i:112XS:i:68Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:5791:792181Test_seq17114460151M=171069-226CTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGTTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:66C84MC:Z:24S127MAS:i:146XS:i:55Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1104:11568:9377	Test_seq	116069	Test_seq	168405	-	+	UR	A00126:224:H7HMWDSX3:3:1104:11568:9377145Test_seq11604721128S23M=16840552337CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCACAAAATGGAGGACAACTACAFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:19SA:Z:Test_seq,168651,-,126M25S,60,1;XA:Z:Test_seq,-85506,127S19M5S,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1104:11568:93772193Test_seq16865160126M25H=168405-372CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:65T60MC:Z:151MAS:i:121XS:i:55SA:Z:Test_seq,116047,-,128S23M,21,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1104:11568:937797Test_seq16840560151M=116047-52337TATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,:FF:FFFFFFFNM:i:1MD:Z:90T60MC:Z:128S23MAS:i:146XS:i:81Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1104:11568:9377	Test_seq	116069	Test_seq	168405	-	+	UR	A00126:224:H7HMWDSX3:3:1104:11568:93772193Test_seq16865160126M25H=168405-372CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:65T60MC:Z:151MAS:i:121XS:i:55SA:Z:Test_seq,116047,-,128S23M,21,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1104:11568:9377145Test_seq11604721128S23M=16840552337CTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCACAAAATGGAGGACAACTACAFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:19SA:Z:Test_seq,168651,-,126M25S,60,1;XA:Z:Test_seq,-85506,127S19M5S,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1104:11568:937797Test_seq16840560151M=116047-52337TATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,:FF:FFFFFFFNM:i:1MD:Z:90T60MC:Z:128S23MAS:i:146XS:i:81Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1104:24921:11741	Test_seq	168602	Test_seq	212800	-	-	UU	A00126:224:H7HMWDSX3:3:1104:24921:11741177Test_seq16845260151M=21265044199TGTTGAATAAGAGCAGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATF:FFFFFFFFFF,FFFFFFF,,:,:,FFFFFFFFFFF,:FF:FFFFFFFFFFF:FF:FFFFFFFFF::FFFF:F:FFFFFFFFFFFFFFFFFF:FFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF::F:FFFF:FFFFNM:i:1MD:Z:14G136MC:Z:151MAS:i:146XS:i:93Yt:Z:UU	A00126:224:H7HMWDSX3:3:1104:24921:11741113Test_seq21265024151M=168452-44199GCAAGGAAGATTTAAAGTTATCACTTTTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCTGGTTATAAAATAAACATACAAAAATCACTTGGATTF,,,,F,,FF,,:FFF,F,FF,:::,FFF,:FF,FFFFFFF:FFFFF:FF,:F::,F,:FF,,:,:,:FF::FFF,:F:F,FF,:F:F,:::F,,:,FF,:FFF:FFF::,FFFFF,FFF:F,:FFFFF:FFF:F,FFF,FFF,FFF:FFFNM:i:11MD:Z:7G2A0G4A5T2A17C0T17T52A8G26MC:Z:151MAS:i:100XS:i:85XA:Z:Test_seq,+168238,151M,14;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1104:8323:13463	Test_seq	170316	Test_seq	170786	+	-	UU	A00126:224:H7HMWDSX3:3:1104:8323:13463161Test_seq17031660151M=170636471AAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAGTAAGTTTTTAGTGCTGTCATTTTGATGFFFFFFFFFFFFFFFFFF::FFFFFFFFFF:FFFFFFFFFFFFFFFF:FF:FFFFFF:FF:FFFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFF,FF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF:FFFF:FFFFFFFF,FFFNM:i:1MD:Z:123A27MC:Z:151MAS:i:146XS:i:52Yt:Z:UU	A00126:224:H7HMWDSX3:3:1104:8323:1346381Test_seq17063660151M=170316-471CTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTTTGATCGTTTATCTATTGATTTCCATGATTFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:3A42G2G3A2A38G25G29MC:Z:151MAS:i:117XS:i:23Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1104:18855:14418	!	0	Test_seq	171064	-	+	NR	A00126:224:H7HMWDSX3:3:1104:18855:1441881Test_seq17129360129M22S=171064-358TTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTACAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTACTCATGGAGACCCCATGTTACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:81T25G15T5MC:Z:151MAS:i:114XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1104:18855:14418161Test_seq17106460151M=171293358ATATATGATCCTTGGTTGGCAGTTTTTCTCCTTCAGTGCTCTGTATACGTCGTCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:1MD:Z:27A123MC:Z:129M22SAS:i:146XS:i:73Yt:Z:NR
@@ -182,7 +182,7 @@
 A00126:224:H7HMWDSX3:3:1105:23077:15562	!	0	Test_seq	167509	-	-	NR	A00126:224:H7HMWDSX3:3:1105:23077:15562161Test_seq1671312225S126M=167359379CCTCCACCCTCTTAAAAATATGTTAAATCTCATCGTAGTTTTAATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:13C98T13MC:Z:151MAS:i:116XS:i:106XA:Z:Test_seq,-213778,126M25S,4;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:23077:1556281Test_seq16735917151M=167131-379CAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF:FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:9A141MC:Z:25S126MAS:i:146XS:i:138XA:Z:Test_seq,+213528,3S148M,2;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:27923:18443	!	0	Test_seq	168451	-	+	NR	A00126:224:H7HMWDSX3:3:1105:27923:1844381Test_seq16868960122M29S=168451-360CTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAATCTACAGTATGATAAAAAAAAAAGTTA:FFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:27T94MC:Z:151MAS:i:117XS:i:54Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:27923:18443161Test_seq16845160151M=168689360ATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTA:FFFF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,:FFF:FFFF,FFFFFFFF:FFFFFF:FF,FFFNM:i:1MD:Z:44T106MC:Z:122M29SAS:i:146XS:i:93Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:8820:20337	Test_seq	171233	Test_seq	171572	+	-	UU	A00126:224:H7HMWDSX3:3:1105:8820:20337161Test_seq17123360151M=171441340TCTTTGGTTTTGGCAAGTTTGATGATAATATATCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFF,FFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFF:FFFFFFFFF,FFFFFFFFNM:i:1MD:Z:31G119MC:Z:132M19SAS:i:146XS:i:36Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:8820:2033781Test_seq17144160132M19S=171233-340CCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCGGCTATGTTGGTGTTGATTTCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTTGAGTTAATTTTTCTACTAFFFFFF:FFFFFF:FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:73A10A7C39MC:Z:151MAS:i:117XS:i:43Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1105:10276:21010	!	0	Test_seq	171288	-	-	MR	A00126:224:H7HMWDSX3:3:1105:10276:21010113Test_seq518770132S19M=171138119394AAAAGAAAGGTTTCCTTCAAGGGAGAATCAATAAGAATAAGTTCAGACTACTCAGCAGAAACCATGCAGGCAAGAAGGGAATGGGATGACGTATACAGAGCACTGAAGGAGAAAATCTGCCAACCAAGGATCGATCAAGTTGGTTGATTGTFFFFFF,,:,,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:19MC:Z:151MAS:i:19XS:i:0SA:Z:Test_seq,171070,+,19S132M,60,3;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1105:10276:210102145Test_seq1710706019H132M=171138219GATCCTTGGTTGGCAGATTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,,:,,FFFFFFNM:i:3MD:Z:16T4A23G86MC:Z:151MAS:i:117XS:i:74SA:Z:Test_seq,51877,-,132S19M,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1105:10276:21010177Test_seq17113860151M=51877-119394TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTGGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:105T45MC:Z:132S19MAS:i:146XS:i:0Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1105:10276:21010	!	0	Test_seq	171288	-	-	MR	A00126:224:H7HMWDSX3:3:1105:10276:210102145Test_seq1710706019H132M=171138219GATCCTTGGTTGGCAGATTTTCTCCTTCAGTGCTCTGTATACGTCATCCCATTCCCTTCTTGCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,,,:,,FFFFFFNM:i:3MD:Z:16T4A23G86MC:Z:151MAS:i:117XS:i:74SA:Z:Test_seq,51877,-,132S19M,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1105:10276:21010113Test_seq518770132S19M=171138119394AAAAGAAAGGTTTCCTTCAAGGGAGAATCAATAAGAATAAGTTCAGACTACTCAGCAGAAACCATGCAGGCAAGAAGGGAATGGGATGACGTATACAGAGCACTGAAGGAGAAAATCTGCCAACCAAGGATCGATCAAGTTGGTTGATTGTFFFFFF,,:,,,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:19MC:Z:151MAS:i:19XS:i:0SA:Z:Test_seq,171070,+,19S132M,60,3;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1105:10276:21010177Test_seq17113860151M=51877-119394TGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTGGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:105T45MC:Z:132S19MAS:i:146XS:i:0Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1105:18837:21089	Test_seq	165754	Test_seq	166211	+	-	UU	A00126:224:H7HMWDSX3:3:1105:18837:2108997Test_seq16575460151M=166061458AGTCAGCCCTTTCAGTCTCTCGTTTCGTGCCAATTTTGCCGTCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTAATTTAATTAGCTCACTCTTCATCAGCATCTCTCTCCCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:3MD:Z:40A5T75G28MC:Z:151MAS:i:136XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:18837:21089145Test_seq16606160151M=165754-458TATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAATTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:82C68MC:Z:151MAS:i:146XS:i:75Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1105:16640:21167	!	0	Test_seq	167661	-	-	NR	A00126:224:H7HMWDSX3:3:1105:16640:2116797Test_seq167316722S129M=167511346TGAACCCTGATTGATTTGGATCGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFF:FF:FFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFF,FFFFFFFF:FFFFFFFFF:FFFFFNM:i:4MD:Z:7A37G6A73T2MC:Z:151MAS:i:111XS:i:106XA:Z:Test_seq,-213590,129M22S,5;Test_seq,+400737,22S123M6S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1105:16640:21167145Test_seq16751160151M=167316-346TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:22S129MAS:i:151XS:i:122XA:Z:Test_seq,+213377,83M2I23M2I37M4S,5;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1105:7835:24173	Test_seq	171202	Test_seq	171530	+	-	UU	A00126:224:H7HMWDSX3:3:1105:7835:2417397Test_seq17120260151M=171381329CTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGCGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:108A42MC:Z:101M1I49MAS:i:146XS:i:76Yt:Z:UU	A00126:224:H7HMWDSX3:3:1105:7835:24173145Test_seq17138160101M1I49M=171202-329TTTTCTCTGTGTTTTCTGTCCCCTCTTCCTGTTCTGGGACTCCAATCACTCACAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTAGGGTTTCTCCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:19G3C2C8T15G46T45A5MC:Z:151MAS:i:108XS:i:28Yt:Z:UU
@@ -215,7 +215,7 @@
 A00126:224:H7HMWDSX3:3:1106:18222:16736	Test_seq	168314	Test_seq	168630	+	-	UU	A00126:224:H7HMWDSX3:3:1106:18222:1673697Test_seq16831411151M=168480317TGGAGGATTCCTTAGGGTTTTCTGTGTATATAATCATGTCATCTGCAAATAGTGATAACTTTACTTCTTCCTTGCCAATCCGGATACCTTTTATTTCTTTGTCTAGCCTGATTGCCCTGGCTAAGACTTCCAACACGATGTTGAATAAGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F:F:FFFFFNM:i:15MD:Z:22C0A6C0G0G18A5G9C12T7C20A13G6A1G0T17MC:Z:151MAS:i:76XS:i:68XA:Z:Test_seq,-212574,151M,17;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:18222:16736145Test_seq16848060151M=168314-317TCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGG:FFFFFFFFFFFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:15T135MC:Z:151MAS:i:146XS:i:91Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:18747:17018	Test_seq	170380	Test_seq	170837	+	-	UU	A00126:224:H7HMWDSX3:3:1106:18747:17018161Test_seq17038060151M=170691458ACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGGGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGAGACGTTTTTFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FFFNM:i:1MD:Z:44C106MC:Z:73M4I74MAS:i:146XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:18747:1701881Test_seq1706916073M4I74M=170380-458CACCTTGTATCACTCTCCATCTGGCAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGATCATTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAATTAATCTFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:40G106MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:16251:18740	Test_seq	225248	Test_seq	308334	+	+	UR	A00126:224:H7HMWDSX3:3:1106:16251:18740129Test_seq2252486029M122S=30833483087GTTCTGATGTTGTTGTCATTTACAGATTATAAGTCAATCTCTTTTGAGATATAAAAGAGAGAAGTGAGGAGAAAGACATGGAGAACTCATGCTACCAAGAAACAAGATCCAGGAGAATAGCATGTCCTTTGGACCCAGGGTCCCCGTGGCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FF,FFFFFFFFFFFFF:FFNM:i:0MD:Z:29MC:Z:151MAS:i:29XS:i:0SA:Z:Test_seq,308589,-,122M29S,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1106:16251:187402193Test_seq30858960122M29H=308334-377AGCCACGGGGACCCTGGGTCCAAAGGACATGCTATTCTCCTGGATCTTGTTTCTTGGTAGCATGAGTTCTCCATGTCTTTCTCCTCACTTCTCTCTTTTATATCTCAAAAGAGATTGACTTAFF:FFFFFFFFFFFFF,FF,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:0MD:Z:122MC:Z:151MAS:i:122XS:i:24SA:Z:Test_seq,225248,+,29M122S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1106:16251:1874065Test_seq30833460151M=225248-83087TCCATAACATACCACTGTAGTGCCCCACATAGAATCCATTTTTATAATATGCTTAGCTTAGTTGTCTTAGTTATCCAGTGCTGCTATAACAGAAATAACACAAGTGGATGGCTTTAACAAACAGAAAGTTATTCTCTACAGTCTAGGAGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:29M122SAS:i:151XS:i:35Yt:Z:UR
-A00126:224:H7HMWDSX3:3:1106:31901:18756	Test_seq	56593	Test_seq	115194	+	+	RU	A00126:224:H7HMWDSX3:3:1106:31901:18756129Test_seq5659360151M=11519458602CTTCCGTATGGATATTATCCTATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCCAAAATGGCCAFFFFFF,,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFFFFF:FFFFFFFFFFF:,FFFFFFFFFFFFF:,FFFFFFF:NM:i:0MD:Z:151MC:Z:5S23M123SAS:i:151XS:i:103Yt:Z:RU	A00126:224:H7HMWDSX3:3:1106:31901:1875665Test_seq115194245S23M123S=56593-58602TGCTCTGTCACATGGGGTTGCTATGAGTAGTCAAGCTTTTGGGACCTTCATTTACTGATATGGCATAACTCAAAATGAGAAGAAATAGCTGCAAACATTCAGTAATAATCAGAATATGGAATGTACAAAATATAAATCTAGGAAAGTTGGAFFFFFFFFF:FF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,56816,-,123M28S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1106:31901:187562129Test_seq5681660123M28H=56593-346TCCAACTTTCCTAGATTTATATTTTGTACATTCCATATTCTGATTATTACTGAATGTTTGCAGCTATTTCTTCTCATTTTGAGTTATGCCATATCAGTAAATGAAGGTCCCAAAAGCTTGACTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:FFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0SA:Z:Test_seq,115194,+,5S23M123S,24,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1106:31901:18756	Test_seq	56593	Test_seq	115194	+	+	RU	A00126:224:H7HMWDSX3:3:1106:31901:18756129Test_seq5659360151M=11519458602CTTCCGTATGGATATTATCCTATCACTGACAGCATTTTACTTCAGGATAGATCTTGAAATGTTCTTTTTGATGATGAATGCAATGCCATTCCTCTTCAAGTTGTCATTCCCAGCATAGTAGACCACATGATTGTCTGATCCAAAATGGCCAFFFFFF,,FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFFFFF:FFFFFFFFFFF:,FFFFFFFFFFFFF:,FFFFFFF:NM:i:0MD:Z:151MC:Z:5S23M123SAS:i:151XS:i:103Yt:Z:RU	A00126:224:H7HMWDSX3:3:1106:31901:187562129Test_seq5681660123M28H=56593-346TCCAACTTTCCTAGATTTATATTTTGTACATTCCATATTCTGATTATTACTGAATGTTTGCAGCTATTTCTTCTCATTTTGAGTTATGCCATATCAGTAAATGAAGGTCCCAAAAGCTTGACTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:FFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:0SA:Z:Test_seq,115194,+,5S23M123S,24,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1106:31901:1875665Test_seq115194245S23M123S=56593-58602TGCTCTGTCACATGGGGTTGCTATGAGTAGTCAAGCTTTTGGGACCTTCATTTACTGATATGGCATAACTCAAAATGAGAAGAAATAGCTGCAAACATTCAGTAATAATCAGAATATGGAATGTACAAAATATAAATCTAGGAAAGTTGGAFFFFFFFFF:FF,FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F,FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:23MC:Z:151MAS:i:23XS:i:0SA:Z:Test_seq,56816,-,123M28S,60,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1106:24795:22357	Test_seq	169327	Test_seq	169680	+	-	UU	A00126:224:H7HMWDSX3:3:1106:24795:22357161Test_seq16932760151M=169530354GCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFF,FFFFF:FFFF:FF:FFFF:FFFFFFFFFFFFFFNM:i:1MD:Z:140G10MC:Z:151MAS:i:146XS:i:111Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:24795:2235781Test_seq16953057151M=169327-354TTCTTCTTTTTGTATGTGTGCATTTATTGATATAAATTGACCTCTGAGCACTGCTTTTGCTGTGTCCCAGAGGTTTTGATAGGAAGTATTTTCATTCTCGTTGCAGCCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:27C11G58T5T0T0T3A22G17MC:Z:151MAS:i:111XS:i:78Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:9127:23187	Test_seq	168739	Test_seq	169233	+	-	UU	A00126:224:H7HMWDSX3:3:1106:9127:23187161Test_seq16873960151M=169083495AACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:81Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:9127:2318781Test_seq16908360151M=168739-495TTCAATCTGTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTACGTAGTAATCTGAAGTGATTFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:7MD:Z:8C62G2T3G7C31C26A5MC:Z:151MAS:i:116XS:i:65Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:17309:23328	Test_seq	168424	Test_seq	168810	+	-	UU	A00126:224:H7HMWDSX3:3:1106:17309:23328161Test_seq16842460151M=168660387ATTGCCCTGGCTACGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCCGTTGGCTTTGCATAGATGCCCTTTATTAF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:13G57T50T28MC:Z:151MAS:i:136XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:17309:2332881Test_seq16866060151M=168424-387ATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:56T94MC:Z:151MAS:i:146XS:i:55Yt:Z:UU
@@ -223,7 +223,7 @@
 A00126:224:H7HMWDSX3:3:1106:27308:24862	Test_seq	167312	Test_seq	214100	-	-	UU	A00126:224:H7HMWDSX3:3:1106:27308:24862177Test_seq16716252151M=21395046789TAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:81T69MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213722,151M,6;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:27308:24862113Test_seq21395037151M=167162-46789AATAAATGTTGGAGAGGCTGCGGAGAGATTGGAACTCTTATACGCTGCTGGTGGGAATGTAAAATGGTACAACCACTTTGGAAATCTATCTGGCGTTTTCTTAAACAGTTAGAAATAGAACTACCATACAACCCAGAAATCCCACTCCTCGF:F:::FFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:28C14A49T11A45MC:Z:151MAS:i:131XS:i:112XA:Z:Test_seq,+166938,4S147M,7;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:2284:29434	!	0	Test_seq	167543	-	-	NR	A00126:224:H7HMWDSX3:3:1106:2284:2943497Test_seq1671734428S123M=167393371GTGAAGATGCATCTACATGCCAAGGATCGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGAC:FFFFF:::FF:F:FFFF::F:,F:,F:FFFFFF:F,:FFFFF:FFFFFFFF:FFFFFFFFF,:FF,FFFF:FF,FFFF,FF,F:F:F:,FFF:FFFF,:FFF:F::F,F,:F,F,FFF:FFFFFFFFF,FFFF:,F::FFF,FFFFFFF,NM:i:2MD:Z:5A64T52MC:Z:151MAS:i:113XS:i:93XA:Z:Test_seq,-213739,123M28S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1106:2284:29434145Test_seq16739360151M=167173-371AGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTAGATCCTATTTTTTCTTCCATGATCTTTATCGTFF:FFFFFFFFF:,,FFFF:,FFFFFFFFFFFFF:FFFFFF,F,FFFF,FFFF:FF:FFF:FF:FF:F:FF,FFFFFFFFF:FFFFFF,:FFFFF,,FFFFFFFF,:FFFFFFFF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:118T32MC:Z:28S123MAS:i:146XS:i:117XA:Z:Test_seq,+213491,29M4D4M4I114M,10;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1106:3125:29763	Test_seq	169989	Test_seq	170460	+	-	UU	A00126:224:H7HMWDSX3:3:1106:3125:29763161Test_seq16998948151M=170310472GGATGTCCTGTCCTTCTCCGAAAGTGGTGTGTTAAAGTCTCCTACTATAATTGTGGAGGTATCTATATCACTTTTCAATTCTGTTAAAATTTGATTTATGTATCTTGCAGCCCTGTCATTGGGTGCATAAATATTTAATATGGTTATTTCT,FFFF:FFF,FF,:FF:F,,FFFFF::F,FF,:F:FF:FFF,FFFFFFF,FFFF,FFFF:FFFFFFFFFFF:FFF:FFFFFF,F:F:F:FFFFFFFFFF,FFF,FF,F,F,,:F,F:FFFF,,FFFFFFFFFFFFFFFFFFF,FFFF,::FNM:i:10MD:Z:33G5C10A7A1G5C2G48C7G20A3MC:Z:151MAS:i:102XS:i:73Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:3125:2976381Test_seq17031060151M=169989-472GTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:66C84MC:Z:151MAS:i:146XS:i:63Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1106:29026:29841	!	0	Test_seq	166159	-	+	MR	A00126:224:H7HMWDSX3:3:1106:29026:2984165Test_seq38347205S21M125S=166159-217314GCCTTGAGTTGCAATAGTGAAGGATTGATCAATCAGGAGATAAGAACTGCTTTGGATGAAGGGAAAGACAACACACAATACAAGGAAGGTCAGCCTAATTGGACTGGACTAAAAGCAAAGAGGTTTCCGGGATAAAATGAAAGCTTCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:21SA:Z:Test_seq,166429,-,128M23S,60,2;XA:Z:Test_seq,-334091,125S26M,1;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:29026:298412129Test_seq16642960128M23H=166159-398CTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCATCCAAAGCAGTTCTTATCTCCTGATTGATCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:95C18A13MC:Z:151MAS:i:118XS:i:28SA:Z:Test_seq,383472,+,5S21M125S,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1106:29026:29841129Test_seq16615960151M=383472217314CAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGGTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:::FFFFFFNM:i:1MD:Z:102T48MC:Z:5S21M125SAS:i:146XS:i:77Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1106:29026:29841	!	0	Test_seq	166159	-	+	MR	A00126:224:H7HMWDSX3:3:1106:29026:298412129Test_seq16642960128M23H=166159-398CTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCATCCAAAGCAGTTCTTATCTCCTGATTGATCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:95C18A13MC:Z:151MAS:i:118XS:i:28SA:Z:Test_seq,383472,+,5S21M125S,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1106:29026:2984165Test_seq38347205S21M125S=166159-217314GCCTTGAGTTGCAATAGTGAAGGATTGATCAATCAGGAGATAAGAACTGCTTTGGATGAAGGGAAAGACAACACACAATACAAGGAAGGTCAGCCTAATTGGACTGGACTAAAAGCAAAGAGGTTTCCGGGATAAAATGAAAGCTTCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:21SA:Z:Test_seq,166429,-,128M23S,60,2;XA:Z:Test_seq,-334091,125S26M,1;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1106:29026:29841129Test_seq16615960151M=383472217314CAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTCCTTTGCCTTTGCTCCAGGTGGGGTGAGACCAATTGATGTATCTTAGATGGCCGCTTGTTGGCATTTAGGACFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF:::FFFFFFNM:i:1MD:Z:102T48MC:Z:5S21M125SAS:i:146XS:i:77Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1106:15591:30123	Test_seq	171134	Test_seq	171455	+	-	UU	A00126:224:H7HMWDSX3:3:1106:15591:3012397Test_seq17113460151M=171323322TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTG:FF:FFFFFF:,FF:F:F,,FF:F:F,F::,FFFFF,FFFF,:::::F:FFFFFFF,,FF::F:F:FFFFFFF:FFFFFF,,F,FFF,:FFFF:F,,:F,F,,,FF:,::FFF,,FF,FF,,FF:F,,,FF,FF,FFFF,F,FFFF,F,:FNM:i:1MD:Z:118T32MC:Z:133M18SAS:i:146XS:i:63Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:15591:30123145Test_seq17132360133M18S=171134-322GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAG::::,FFF,:F:FFF,FFF:FF,:FF:FF:FF:FF,F:,F:F:::FF::F,FFFFF,,,,,FF:F::::F,,FF,F,FFFFFF:FFF,F:FF:F::FF,FF,FFF,F,FFFFFF,F,:FF,FFFF:F,FFFFFF:FF::FFFFFF:FF:,FNM:i:3MD:Z:46G30G15T39MC:Z:151MAS:i:118XS:i:38Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:30138:30483	Test_seq	183328	Test_seq	183714	+	-	UU	A00126:224:H7HMWDSX3:3:1106:30138:30483161Test_seq1833286026M4I121M=183564387TGTTCTGCATCTATTAAAAATGATGAGATCGATCCATGTAGCTGTAGCAGTGGTGGGTTAGAGTTTTAGGTCTGTAGCCAGTAGATGTATGTTTGTATTTTCTCTCTGCCACTTACTAGTTGTGTTAATTTGAGCAAGTCATTTGACCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFF::FFFFFFNM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:30138:3048381Test_seq18356460151M=183328-387TGGTTCCGACTCATAGCATCCCTATGTACAACAGAATGAAACGCTGCCCTGTCCTGTGCCAGCCTTGCAATCATTGTTATGCTTGATCCCATTGTTGTAGCCACTGTATCAATCCATCTCATTGAGGGTCTTGCTCTTTTTCACTGACCCTFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:,FFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFF:FFFFF:FFFFFFF:FFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:26M4I121MAS:i:151XS:i:52Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:15890:30639	Test_seq	171134	Test_seq	171455	+	-	UU	A00126:224:H7HMWDSX3:3:1106:15890:3063997Test_seq17113460151M=171323322TGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTCGATGATAATATGTCTTGGTGTTTTTCTTTTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,::FFFFFFFFFFFF,:FF:F:FFFF,FFFFFF:FF:FFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:118T32MC:Z:133M18SAS:i:146XS:i:63Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:15890:30639145Test_seq17132360133M18S=171134-322GATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGATTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTAATCATGGCCCAGCCAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF:FF,:FFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:46G30G15T39MC:Z:151MAS:i:118XS:i:38Yt:Z:UU
@@ -232,7 +232,7 @@
 A00126:224:H7HMWDSX3:3:1106:14362:33599	Test_seq	167148	Test_seq	167571	+	-	UU	A00126:224:H7HMWDSX3:3:1106:14362:3359997Test_seq16714841151M=167421424AATTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF::FF,FFFF:FFF:FFFFFF,F,FFFFFFF,F:FFFF,:FFFNM:i:2MD:Z:30A64T55MC:Z:151MAS:i:141XS:i:121XA:Z:Test_seq,-213736,151M,6;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:14362:33599145Test_seq16742160151M=167148-424TTTTAGGAGCTCCCAGTTATCTGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTGGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFF:FF:,FFFFFFFFFFFF:FFFFF:F,FFFFFFNM:i:1MD:Z:85A65MC:Z:151MAS:i:146XS:i:106Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:27236:35697	Test_seq	406947	Test_seq	407376	+	-	UU	A00126:224:H7HMWDSX3:3:1106:27236:35697161Test_seq4069476061M4I86M=407226430TCCAGGGACCTCAGCTATTCCATGAGATAATTTTACAAATGGTAGCCAGTGTCCTGTTTCAGATCGATCACAAATGTCCCTGTGGCCCCACTAGTGTCTTAAACATTATATAGGAACAAGGCTTTGCAGGGCAAAGACAGCAGTCCACTAGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFF:F:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:147MC:Z:151MAS:i:137XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:27236:3569781Test_seq40722660151M=406947-430GTGCAGCTTGTCCTTGCTCTTCAACTTTGTGTTGAGAAGTAAATAATAAGCTTGAATGCTCTTTTGCAGATAATCCTAACAGGGGAACCAACACTATAGCTAGAGATTCAGGAGAATTTGAAAAGAGGTTTACTCATTTGGAAATGGGAGAF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:61M4I86MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1106:29008:36886	Test_seq	167972	Test_seq	168330	+	-	UU	A00126:224:H7HMWDSX3:3:1106:29008:36886161Test_seq16797237151M=168180359TCGGAAGTGCGTTAAATGTATAGATGGCATTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTAGTAGAGCTTTGTAGTTTTCTTTCTATAGFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,F:FFFF:FF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:10MD:Z:1T12T13T50G8C35C4T3A11G1G3MC:Z:151MAS:i:108XS:i:86Yt:Z:UU	A00126:224:H7HMWDSX3:3:1106:29008:3688681Test_seq16818042151M=167972-359TGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:F:FFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:1MD:Z:91T59MC:Z:151MAS:i:146XS:i:126XA:Z:Test_seq,+212708,151M,5;Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1107:20654:1313	Test_seq	382625	Test_seq	435035	-	-	UR	A00126:224:H7HMWDSX3:3:1107:20654:1313177Test_seq38260035125S26M=43488552411TGGAATTCTAGGTCTTCAAAGTGTTTGCTCTCAACATCCAACAGGTCAGCATCCTTTGGGGAAAAGGGAATGAAAACAAGACACACCAGAATGGCTGTAGCCTTAACATGAAGAAGCAATTATTATAATATGACTATTATTCAAATTTACTFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A0MC:Z:151MAS:i:25XS:i:0SA:Z:Test_seq,434625,+,26S125M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1107:20654:13132209Test_seq4346256026H125M=434885411TAATAATTGCTTCTTCATGTTAAGGCTACAGCCATTCTGGTGTGTCTTGTTTTCATTCCCTTTTCCCCAAAGGATGCTGACCTGTTGGATGTTGAGAGCAAACACTTTGAAGACCTAGAATTCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:125MC:Z:151MAS:i:125XS:i:0SA:Z:Test_seq,382600,-,125S26M,35,1;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1107:20654:1313113Test_seq43488560151M=382600-52411AAAAAAAAAAAAATTAAAATACACTCCTTGTATGTGACAGTAAAGATGGTCAGTATAGCTTTAACGTGTTATCTGTATGTCTGCATGTCTTTGTACCTGAACACCTTTGTGTATTTAATCACATATGGTATTTGGCAGACTGTGGATTCTGFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:125S26MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1107:20654:1313	Test_seq	382625	Test_seq	435035	-	-	UR	A00126:224:H7HMWDSX3:3:1107:20654:13132209Test_seq4346256026H125M=434885411TAATAATTGCTTCTTCATGTTAAGGCTACAGCCATTCTGGTGTGTCTTGTTTTCATTCCCTTTTCCCCAAAGGATGCTGACCTGTTGGATGTTGAGAGCAAACACTTTGAAGACCTAGAATTCCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFNM:i:0MD:Z:125MC:Z:151MAS:i:125XS:i:0SA:Z:Test_seq,382600,-,125S26M,35,1;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1107:20654:1313177Test_seq38260035125S26M=43488552411TGGAATTCTAGGTCTTCAAAGTGTTTGCTCTCAACATCCAACAGGTCAGCATCCTTTGGGGAAAAGGGAATGAAAACAAGACACACCAGAATGGCTGTAGCCTTAACATGAAGAAGCAATTATTATAATATGACTATTATTCAAATTTACTFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A0MC:Z:151MAS:i:25XS:i:0SA:Z:Test_seq,434625,+,26S125M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1107:20654:1313113Test_seq43488560151M=382600-52411AAAAAAAAAAAAATTAAAATACACTCCTTGTATGTGACAGTAAAGATGGTCAGTATAGCTTTAACGTGTTATCTGTATGTCTGCATGTCTTTGTACCTGAACACCTTTGTGTATTTAATCACATATGGTATTTGGCAGACTGTGGATTCTGFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:125S26MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1107:4788:1892	Test_seq	166087	Test_seq	166508	+	-	UU	A00126:224:H7HMWDSX3:3:1107:4788:189297Test_seq16608760151M=166358422GGAGTTGTCTGTTGTGTTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGGCCTTGGTGTTCTTFFFFFFFFF:F:FFFF:FFF:,FFFF:FFF,FFFFFFFFFFFFFFFFFFFF,FF,FFFF::FFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF,FFFFFFFFFF:FFFF,FFFFFFFFFFFF,F:FFFFFFFFNM:i:1MD:Z:16C134MC:Z:151MAS:i:146XS:i:74Yt:Z:UU	A00126:224:H7HMWDSX3:3:1107:4788:1892145Test_seq16635860151M=166087-422ATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTG,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:43Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1107:7120:2801	Test_seq	165764	Test_seq	166210	+	-	UU	A00126:224:H7HMWDSX3:3:1107:7120:280197Test_seq1657646016S135M=166060447ACTTGCTCTCCCCCTATTCAGTCTCTTGTTTCGTGCCAATTTTGCCATCTTCCCTCTCTCTCTATCTTCCCATCCCCCCTCCAGTCAAGAGTTGCCAACACACTCTCCAGTGTCCACCTGATTTAATTAGCTCACTCTTCATCAGCATCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:10C25T66A8G22MC:Z:151MAS:i:115XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1107:7120:2801145Test_seq16606060151M=165764-447GTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAAGGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:107T43MC:Z:16S135MAS:i:146XS:i:75Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1107:29279:4225	Test_seq	167906	Test_seq	168348	+	-	UU	A00126:224:H7HMWDSX3:3:1107:29279:422597Test_seq16790660151M=168198443TTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:41Yt:Z:UU	A00126:224:H7HMWDSX3:3:1107:29279:4225145Test_seq16819839151M=167906-443GGTTATTTCCTCTTCGATGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTTTTCTATTAGTTTCAGTAGTTTTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATC:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFNM:i:4MD:Z:16G56T18C55G2MC:Z:151MAS:i:133XS:i:113XA:Z:Test_seq,+212690,151M,8;Yt:Z:UU
@@ -274,7 +274,7 @@
 A00126:224:H7HMWDSX3:3:1108:9634:5588	Test_seq	40166	Test_seq	212883	+	-	UU	A00126:224:H7HMWDSX3:3:1108:9634:5588161Test_seq40166179M3I69M=212733172718CAACAGCCAACATCACCCTAAATGGGGAGAGACTGAAAGCATTCCCCTTGAGAACGGGAACCAGACAAGGATGCCTTTTATCACCACTCGTATTCAACACTGTGCTAGAGGTCCTAGCCAGAGCAATTAGGCTAGGCAAAGAAATAAAGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFF:FFFFFFNM:i:16MD:Z:15T9A5C11T21G9C5T0G3T9T6G28A0T14MC:Z:151MAS:i:74XS:i:71XA:Z:Test_seq,+212488,151M,16;Test_seq,-168400,151M,17;Test_seq,-50359,64M13I74M,24;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:9634:558881Test_seq21273360151M=40166-172718CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTF,F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:104A46MC:Z:79M3I69MAS:i:146XS:i:116Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:16071:6872	Test_seq	36103	Test_seq	167548	+	+	UR	A00126:224:H7HMWDSX3:3:1108:16071:6872129Test_seq361031026M125S=167548131446TGCAAAGGCAATTCTGATAGGGATCTAAGGGAATAGAGAAACAAATCACTAATTTCATATGGAAAGGAAAGAACCCCCGGATAAGCAAAACATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTCFFFF:FFFF:::FFFFFFFFFF::F,,FFFFF,::F:FF::FF,FFFFFF,FFFFFF,FFFF,,FFFF,FFF::FFFFFF:F,,FFF::FF:F::FFFF,F:FFF:F,:FFF,F:FFFFFFFFF:FF,F,,F:FF,:,FFFFF,FFF,F:,NM:i:1MD:Z:20T5MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,167824,-,126M25S,60,4;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1108:16071:68722193Test_seq16782460126M25H=167548-402GAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGTTTTGCTTATCCGGGGGTTCTTTCCTTTCCATATGAAATTAGTGATTTGTTTCTCTATTCCCTTA,:F,FFF,FFFFF,:,FF:F,,F,FF:FFFFFFFFF:F,FFF:,F:FFF:F,FFFF::F:FF::FFF,,F:FFFFFF::FFF,FFFF,,FFFF,FFFFFF,FFFFFF,FF::FF:F::,FFFFF,,NM:i:4MD:Z:0T60C24C32C6MC:Z:151MAS:i:110XS:i:75SA:Z:Test_seq,36103,+,26M125S,10,1;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1108:16071:687265Test_seq16754842151M=36103-131446GTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATTATTTCCCCAATTGACTGACACTGF:F,FFFFFFF::FFFFFFFF:F:,:FFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF,FFF:F::FFFFF:FFF:FFFFF::,FFFF,F:F:::FFF,FF,::F:F:F,:FFFFFF:F:FF::F,FF:FFF:::,,,FFF::FFF:::F:NM:i:1MD:Z:127C23MC:Z:26M125SAS:i:146XS:i:126XA:Z:Test_seq,-213340,120M2I24M5S,4;Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1108:7292:7576	Test_seq	167298	Test_seq	167624	+	-	UU	A00126:224:H7HMWDSX3:3:1108:7292:7576161Test_seq1672982119S132M=167474327ATGAGGGTAATGATGATTAAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFNM:i:2MD:Z:25A44A61MC:Z:151MAS:i:122XS:i:112XA:Z:Test_seq,-213605,132M19S,4;Test_seq,+400719,19S132M,6;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:7292:757681Test_seq16747460151M=167298-327GTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:19S132MAS:i:151XS:i:104Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1108:24126:7701	Test_seq	55744	Test_seq	65171	+	-	RU	A00126:224:H7HMWDSX3:3:1108:24126:770197Test_seq5574460151M=651449428TCTCAACAGAGAAGCTGCCAAAACACTGAAAATCTTGGCCTGGCCAATTTGACACACAATCTTAACCATCACAAATAGTTCGTCTTCTTGAGCAGTCCTTTGAAATCTTCTGTTCAGCTCTTTTACTTAATCATTTCTTCCTTTCAATTTAFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:151MC:Z:123S28MAS:i:151XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:24126:7701145Test_seq6514454123S28M=55744-9428TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTATAAGAACCAGAGGTCAGATGATGACGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:28MC:Z:151MAS:i:28XS:i:0SA:Z:Test_seq,56015,-,123M28S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:24126:77012193Test_seq5601560123M28H=55744-394TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:23SA:Z:Test_seq,65144,-,123S28M,54,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1108:24126:7701	Test_seq	55744	Test_seq	65171	+	-	RU	A00126:224:H7HMWDSX3:3:1108:24126:770197Test_seq5574460151M=651449428TCTCAACAGAGAAGCTGCCAAAACACTGAAAATCTTGGCCTGGCCAATTTGACACACAATCTTAACCATCACAAATAGTTCGTCTTCTTGAGCAGTCCTTTGAAATCTTCTGTTCAGCTCTTTTACTTAATCATTTCTTCCTTTCAATTTAFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:151MC:Z:123S28MAS:i:151XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:24126:77012193Test_seq5601560123M28H=55744-394TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:123MC:Z:151MAS:i:123XS:i:23SA:Z:Test_seq,65144,-,123S28M,54,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:24126:7701145Test_seq6514454123S28M=55744-9428TTCCACACTCATCCGGTATTGGGTCATCAGTGTTCAATGCATGAAATTATTCTTGAGACAGTCTCTAAATTCAGGTGGGATATGCTCAACATTGTGTACTTTGGCTCTCGTGGATTTGTTTTATAAGAACCAGAGGTCAGATGATGACGAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:28MC:Z:151MAS:i:28XS:i:0SA:Z:Test_seq,56015,-,123M28S,60,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1108:21061:8187	Test_seq	168347	Test_seq	168776	+	-	UU	A00126:224:H7HMWDSX3:3:1108:21061:8187161Test_seq1683476017S134M=168626430AAATGTTTGAACTTAGATCATGTCATCTGCAAATAGTGATAGCTTTACTTCCTCCTTACCAATCTGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::FFFFFFFFFNM:i:2MD:Z:18A21G93MC:Z:151MAS:i:124XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:21061:818781Test_seq16862660151M=168347-430ATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:90T60MC:Z:17S134MAS:i:146XS:i:72Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:18909:8469	Test_seq	104742	Test_seq	114432	+	-	UR	A00126:224:H7HMWDSX3:3:1108:18909:846997Test_seq1047426031M120S=1142829691CACATATCAGGTGTCAACAGGGAAGTGATCAATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTTFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:31MC:Z:151MAS:i:31XS:i:0SA:Z:Test_seq,114039,+,30S121M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1108:18909:84692145Test_seq1140396030H121M=114282394AATCCACAAAACATCTCACAACGTGGATGAATCTGGAGGGCATTATGCTGAGTGAAATAAGTCATTCACAAAAAGACAAATAATGTGTGAGATCACTATTATAAGAACTCAAGAAAAGGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:121MC:Z:151MAS:i:121XS:i:73SA:Z:Test_seq,104742,+,31M120S,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1108:18909:8469145Test_seq11428260151M=104742-9691CAGCACAACTGGGCCAAAGCAAAACAAAGAAATTTCCTGGGCACATCTAAACACTGCAAGGGACAGAGTAGCTGGGGTTGGCGATTGGGGACCATAGTTTTGGGGAACATCTAGGTCAACTGCAATAATGAAGTTTATTAATAAAATGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:81T69MC:Z:31M120SAS:i:146XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1108:14498:8656	Test_seq	171559	Test_seq	171939	+	-	UU	A00126:224:H7HMWDSX3:3:1108:14498:865697Test_seq17155960151M=171789381ACATTCTAATTGCTCAAGTCTGCTCCTCTGACTTTCTATTGCATTGTCAAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGATGTCTCTCTACGGATTCTTGCAACTTATTAATTTTTCCACTATGTTCTTGAATAATCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFF,FF:FFFFFFF,FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:F:FFFFFF:FFFFFF,FFFFFFFFFFFFF,FFFF:FFFNM:i:8MD:Z:15G8T17G5T41C10T30C13C4MC:Z:151MAS:i:111XS:i:67Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:14498:8656145Test_seq17178960151M=171559-381GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTGFFFFFFF:FFFFFF,FF:FFFFFF:FF::FFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFNM:i:1MD:Z:149C1MC:Z:151MAS:i:149XS:i:0Yt:Z:UU
@@ -291,7 +291,7 @@
 A00126:224:H7HMWDSX3:3:1108:30544:19789	!	0	Test_seq	167661	-	-	NR	A00126:224:H7HMWDSX3:3:1108:30544:1978997Test_seq167316722S129M=167511346CCGTGGGTATTGATTGTGGATCGATCAGGCGCTGGTCTGAGATATCATAGCTGAATATTCTTTCCCAATCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGTTGTTTGATTTTTAGGAGCTCCCAGTTATCTGG:FFF:F:FFFF,F,FF:::FFF,F,FFF,FFF::FFFFFFFFFFFFFFFFFFFFFF,FF,:F:F,FFFFFFF:F,:FFFF:FFFFFF:FFFFFFF,F:FF:,FF,F:F:FFFFFFFFF,FF,FFF:FF:FFF:FF,FFFFF:F,F:FFF,FNM:i:5MD:Z:7A13G23G6A43G32MC:Z:151MAS:i:104XS:i:99XA:Z:Test_seq,-213590,129M22S,6;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:30544:19789145Test_seq16751160151M=167316-346TGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTCAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGFFFF::FFFFFFFFF:F,F,FFFFFFFF,FFFFFFFFFFFFF,FFFFFF,FFFFFFFFFFF::::FF:FFF:FFFFFF:FF:F:FFFFFFFFFFFFFF,FF::FFFF:FFFF:FFFFFFFFFFF::FFFFFFF::FFFFFFFFFFFF,FF:NM:i:1MD:Z:48T102MC:Z:22S129MAS:i:146XS:i:117XA:Z:Test_seq,+213377,83M2I23M2I37M4S,6;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1108:8187:20400	Test_seq	77443	Test_seq	233514	+	+	RU	A00126:224:H7HMWDSX3:3:1108:8187:2040065Test_seq7744360151M=233514156072TCCTCAGACAGTGCAATTTTCCTTGGCTTTTCAGCCCATAAAATTACTTGATAAACACTGTCTGCTTCTAAGCTTTCGGCCTTACACAGTTGCTTTGCTCCATTGTAAATAATTAAGAATTTAACAGGCCACTGATTAAAAACAAAATTGGFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,,:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:::F:FFFFF:F,FFFF:F:FFFF:F:FFNM:i:1MD:Z:42G108MC:Z:27M124SAS:i:146XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:8187:20400129Test_seq2335144827M124S=77443-156072CTCAAACCCATTGCCGTCGAGTTGATTACTCCATATTGGCAAGTGCAGCAAGGCTGGGGCCCCTTCAGGACATATAGAATATAGCCAACATGAGGCCCAGGGTCAAGATCAGGGGGCCATTTGGTAGCCTCCAAAAGCAAAGACTAACATCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFF:FFFFF:FFFFFFFFFFFF:FFFFFF::FFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFNM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,77734,-,124M27S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:8187:204002193Test_seq7773460124M27H=77443-415GATGTTAGTCTTTGCTTTTGGAGGCTACCAAATGGCCCCCTGATCTTGACCCTGGGCCTCATGTTGGCTATATTCTATATGTCCTGAAGGGGCCCCAGCCTTGCTGCACTTGCCAATATGGAGTFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFF::FFFFFF:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFNM:i:0MD:Z:124MC:Z:151MAS:i:124XS:i:0SA:Z:Test_seq,233514,+,27M124S,48,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1108:8495:20494	!	0	Test_seq	162287	-	+	NR	A00126:224:H7HMWDSX3:3:1108:8495:20494145Test_seq16242260126M25S=162287-261CTCAAAAACCCTATGGAAGAGTTCTTCTGTGCACACATGGGGTGGTCACGAGTCAGAATCGACTGGACTAGCAACTAACAACAGCAGGCAAGTTCGAAGGGTCCTCCTGGGTAGTTAGATGGGATCGATCTAATCTCTAAAATCTGTAGGAF:,F:F:FFFFFFFFFF,F:FFFFF:,FFFFFFF:F:FFFFFFFFFF,FF,:FF::,FFFF:FFFFFFFFFFFFFFFF:FFF:,FFF:F,FFFFFFFFFFFFF:FFFFFF,FFFFFFF:FFFFF:FFFF,,,FFFFFF:F::FFFFFFF:FNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:8495:2049497Test_seq16228760151M=162422261GGAGGAGGCAGGCAGAGGGAGGAGAAAAGCTTATTGGCTTATCATGGCATTAGTCATGTCAAGCTCACAGACACCCATTTCGAAAAGGAGAAAACAAGCTGGGGAGTATTAGGGTCACATTCTGAAAGGTAGAGCCTCAAAAACCCTATGGFFF:F:F,FFFFFFF:FFF:FFF:FFFFFF,FFFF:FF:FFF:FF::F:FFFF:FFFFFFFFFF:F,FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFFFFF::FFFFF,FF:FFFFFFFFFFFFFFFFFFF:,FFFFFFFFFF:FFFFFFNM:i:1MD:Z:66C84MC:Z:126M25SAS:i:146XS:i:0Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1108:7744:20509	Test_seq	169333	Test_seq	170167	+	-	RU	A00126:224:H7HMWDSX3:3:1108:7744:2050997Test_seq16933360151M=170144835GGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:F,FFFFF:F:FF:NM:i:1MD:Z:134G16MC:Z:127S24MAS:i:146XS:i:109Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:7744:20509145Test_seq17014430127S24M=169333-835CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCAATTGTCCCTTTTATCATTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,169626,-,121M30S,60,4;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:7744:205092193Test_seq16962660121M30H=169333-414CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:4MD:Z:2T11A22G70A12MC:Z:151MAS:i:103XS:i:0SA:Z:Test_seq,170144,-,127S24M,30,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1108:7744:20509	Test_seq	169333	Test_seq	170167	+	-	RU	A00126:224:H7HMWDSX3:3:1108:7744:2050997Test_seq16933360151M=170144835GGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFF,F:F,FFFFF:F:FF:NM:i:1MD:Z:134G16MC:Z:127S24MAS:i:146XS:i:109Yt:Z:RU	A00126:224:H7HMWDSX3:3:1108:7744:205092193Test_seq16962660121M30H=169333-414CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:4MD:Z:2T11A22G70A12MC:Z:151MAS:i:103XS:i:0SA:Z:Test_seq,170144,-,127S24M,30,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1108:7744:20509145Test_seq17014430127S24M=169333-835CTCGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCAATTGTCCCTTTTATCATTAFF:FFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,169626,-,121M30S,60,4;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1108:16233:21339	Test_seq	197163	Test_seq	197474	+	-	UU	A00126:224:H7HMWDSX3:3:1108:16233:21339161Test_seq19716360151M=197343312TCACATTTTTCTCCCAGCATACGCATGCTGATGTGGGGTTTTGTCATGTTTCTTAAGGGAACTGTGATGAAAATTCTCCTCTGACAGACATGTCTCAACTTCCAGATGGTAAGAAATTTTGTGAGAGTGATGAAATTAAGATACTTGATGCFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFF:F:FFFFFFFFFFFFFFF,FFFFFFFFFF:,F:FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:132M19SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:16233:2133981Test_seq19734360132M19S=197163-312ATTCTAGGGGGAATTGCTTCCATGACAGTGTATTGAACCTGTGCTCCAAGGGGTGCCTTTAACAGTGGACTTGGTATCCTCTGGAGTTGTACAACGTAATTGCTCTGAGCCCAGAGCCCAGGAAATAAGATCGATCTTGATTTTCAGTACGFFF:,FF:FFFFFFF:FFFF:FFFFF:FFF:FFFFF:::FFFFFFF:F,FF:FFF::FF:FF,FFFFFF:FFFFFFFFFFFFFFFFFF:FFF::FF:FFFFFFFFFFFFFF::FFF:FF:FFF,FFFF:FFFFFFFFFF,FF:FFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1108:16342:23437	!	0	Test_seq	171131	-	+	NR	A00126:224:H7HMWDSX3:3:1108:16342:23437145Test_seq17129460128M23S=171131-291TAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTTAATGGATGTGTGCAGCTTTTCT:FFF:,FFFFFFFFFF:FFF,FFFFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FF:FF:FFF:FFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFF:FFFFF:FFFFFNM:i:2MD:Z:106G15T5MC:Z:151MAS:i:118XS:i:43Yt:Z:NR	A00126:224:H7HMWDSX3:3:1108:16342:2343797Test_seq17113160151M=171294291GCCTGCATGGTTTCTGCTGAGTAGTCTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTATCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFF:FFFFFFFFF::FFFF,FF,:FFF,FFFFFFFFFFFFF:FFFFFFFF,FF:F:FFF,FFFFF:FFFFF:FF:NM:i:1MD:Z:71C79MC:Z:128M23SAS:i:146XS:i:75Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1108:16740:23625	Test_seq	170381	Test_seq	170830	+	-	UU	A00126:224:H7HMWDSX3:3:1108:16740:23625161Test_seq17038160151M=170680450CTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTTTCATGTGTGTTGTTGGCCTTTTCATTTTTCCACATACTTTTTTGTGCTGCGACGTTTTTCF:FFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF::F:FFFFF:F,FF:FFFFFFF:FF:NM:i:1MD:Z:140A10MC:Z:151MAS:i:146XS:i:47Yt:Z:UU	A00126:224:H7HMWDSX3:3:1108:16740:2362581Test_seq17068060151M=170381-450GCGTTCTATATCACCTTGTATCACTCTCCATCTGGTAGTTCAATGCCTCCTATATTTAGTCCCTCTTTTTGATTATTGTGATCGTTTATCTATTGATTTCCATGATTTCCTGTTATGTGTATTATTTTGTTTATTTATTTATTTTTTAGAAF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:4MD:Z:0T4G29C15G99MC:Z:151MAS:i:135XS:i:0Yt:Z:UU
@@ -339,7 +339,7 @@
 A00126:224:H7HMWDSX3:3:1109:31367:22592	Test_seq	167451	Test_seq	213168	+	+	UU	A00126:224:H7HMWDSX3:3:1109:31367:22592129Test_seq1674515481M4I66M=21316845718TTCATCATTTTTGGTAATGTTTTGTGTTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGTCCCTATTTTTTCTTCCATGATCGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATGCACTTGGAGTTAGTTTTTGTGCATGGFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFF:FFFFFF,F:FFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:8MD:Z:25A36A0T56C26MC:Z:151MAS:i:117XS:i:87Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:31367:2259265Test_seq2131687151M=167451-45718AAGAAGAAAGTGGGAGGCCTCACTCTACCTGATTTCAGAACCTATTATACAGCTACAGTAGTCAAAACAGCCTGGTACTGGTACAACAACAGGCACATAGACCAATGGAACAGAATTGAGAACCCAGATATAAATCCATCCATGTATGAGCF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:144A6MC:Z:81M4I66MAS:i:146XS:i:141XA:Z:Test_seq,-167720,151M,2;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:14751:22748	Test_seq	171454	Test_seq	171939	+	-	UU	A00126:224:H7HMWDSX3:3:1109:14751:2274897Test_seq1714546028M1I122M=171789486GTCCCACATGATTCTTAGGGTTTCTTCATTTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGTGTCGATTCCCTGGTCCTCCAGATGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTCCTATTGCGTTFFFFFFFFFF,FFF,:FFFFFFFF:FFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FF:FFFFFFFFFFF:FF:FFFFFFFFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF,FNM:i:6MD:Z:71A2T19A34T9T10MC:Z:151MAS:i:118XS:i:32Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:14751:22748145Test_seq17178960151M=171454-486GCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGTTGGAGAAGCTGTCATGGTCTGTTTCTTTATGTGGTTTGATATGGACTGCTGTCTCTG:FFF::FFFFFFFFF:FFFFFF,F,:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF,FFFFFFFF:FFFF:FFFFFF:FFFFFFFFFFFFFFFFF:,FFF:FFFFFFFFFFFFNM:i:1MD:Z:149C1MC:Z:28M1I122MAS:i:149XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:32579:24314	Test_seq	132878	Test_seq	133237	+	-	UU	A00126:224:H7HMWDSX3:3:1109:32579:2431497Test_seq13287860151M=133104360GACGAGCCTTGAACGTGTGAGGCTCAGTACTGCTGTGTCTTTCACGTGCGCCGCTGTATTTGTGGCCTCTGTCTCCAGGGGAAGACTGTATTCGCAGCCCCTCATTCTCAGGGTACAACATTTTGTCTTCCGAGCAGCTGGATGTGAAGGCFFFFFFFFF:FFFFFFFFFFFFF,FFFFFF:FFFFFFFF,FFFFFF:FFFFFFFFFF,F:FFFFFFF,FFF,F:FFFFFFFF,FFF:FF:,FFFFFFFFFFFFFF:FFFFFFF:,F,,FF:F:FFFF:FFFFFFF:FF:FFF,FFF,FFFFNM:i:0MD:Z:151MC:Z:134M17SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:32579:24314145Test_seq13310460134M17S=132878-360TCAAACACTTATACACCAAGTCCTCACTTATCAACATGTTTAGGTTCCAAAGTCTAGGTTGTTATACAAAAATCGTCATTAAGCAAAAATGGTAGATGACTACAACATTACATAACTGCCAGACCACTAAGAATTAAAGTGACAGCTTGAAFFFFFFFFF:F::F,FF:F,FFFFF:F:,FFF::F::FF,,FFF,FFFF,:FFF:FFFFFFF:FFFFFFF:FFFFFFFF:,FFFF:FFFFFFFFFF,FFFF,F:FFF::FF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFF:F,FF:F:FFNM:i:1MD:Z:19T114MC:Z:151MAS:i:129XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1109:17246:27352	Test_seq	81985	Test_seq	97460	+	-	RU	A00126:224:H7HMWDSX3:3:1109:17246:2735297Test_seq8198560151M=9744015476TTTTTGTTATTGTGAACAATGCAGTGAACATTGGTGTGCATATGTCTGTTTGTGTGACGGCTCTTATTTCTCTAGGATATATTTCTAGGAATGGGATTGCTGGATCATACGGTATTTGTATTTGTAGCTTTTTAAGGAAGCACCATATCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:130S21MAS:i:151XS:i:81Yt:Z:RU	A00126:224:H7HMWDSX3:3:1109:17246:27352145Test_seq9744012130S21M=81985-15476GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGATCCAGTTCTTTTAGAAGCCFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,82184,-,132M19S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1109:17246:273522193Test_seq8218460132M19H=81985-331GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:21SA:Z:Test_seq,97440,-,130S21M,12,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1109:17246:27352	Test_seq	81985	Test_seq	97460	+	-	RU	A00126:224:H7HMWDSX3:3:1109:17246:2735297Test_seq8198560151M=9744015476TTTTTGTTATTGTGAACAATGCAGTGAACATTGGTGTGCATATGTCTGTTTGTGTGACGGCTCTTATTTCTCTAGGATATATTTCTAGGAATGGGATTGCTGGATCATACGGTATTTGTATTTGTAGCTTTTTAAGGAAGCACCATATCATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFF:F,FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:130S21MAS:i:151XS:i:81Yt:Z:RU	A00126:224:H7HMWDSX3:3:1109:17246:273522193Test_seq8218460132M19H=81985-331GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:132MC:Z:151MAS:i:132XS:i:21SA:Z:Test_seq,97440,-,130S21M,12,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1109:17246:27352145Test_seq9744012130S21M=81985-15476GTTCCTGTCTCCTCGCAGCCTCTCCAGCATTTGTTATTTTCTGTTTTTTTTTGTGTCATGCCAGTAATGTCAGGATGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTTAATGACTAGTGATCGATCCAGTTCTTTTAGAAGCCFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFNM:i:0MD:Z:21MC:Z:151MAS:i:21XS:i:0SA:Z:Test_seq,82184,-,132M19S,60,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1109:17788:27821	Test_seq	168737	Test_seq	169220	+	-	UU	A00126:224:H7HMWDSX3:3:1109:17788:27821161Test_seq16873760151M=169070484TAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGACCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTCTATTGGCTAGAATTTTGTTGAGGATTTTTGCATCTATGTTCATGAGGGATATAGGTCTAAAATTTTCTTTTTTTGTFFF,FFFFFFFFFFFF:FFFFFFF:FFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:F,FFFFFFFFFFFF:FFFFFFF,FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FF,F,FFF:FFFFF::FFFNM:i:1MD:Z:38T112MC:Z:151MAS:i:146XS:i:81Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:17788:2782181Test_seq16907060151M=168737-484TTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTACTTCTGAATGTGTTAGTTTAGGTAGGTAGTATTGTTCCAAGAATTTATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAGTTTTACATAGTAFFFFFFFF::,FFFFFFFFFFFFFFF:FFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFNM:i:7MD:Z:84G2T3G7C31C6A6G5MC:Z:151MAS:i:116XS:i:66Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:12346:31078	Test_seq	171229	Test_seq	171627	+	-	UU	A00126:224:H7HMWDSX3:3:1109:12346:31078161Test_seq17122960151M=171476399TTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATCATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACT:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFF::FFF:FFFFFF:F:FFFFFF:FF:FFFFFFFFFFFFFNM:i:1MD:Z:114G36MC:Z:19M1D132MAS:i:146XS:i:27Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:12346:3107881Test_seq1714766019M1D132M=171229-399TCTTCATTTTTTTAAATTCTTTATCTGATTTTTTTTCAGCTATGTTGGTGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTTCTCTGACTTTCTATTGTGTTGTCTAATTCTGTAATTTTATTGTT:FFFFFFFFF:FFFFFF:FFFFFFFFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:13T5^T29A74C27MC:Z:151MAS:i:129XS:i:26Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1109:30852:31782	Test_seq	166084	Test_seq	166553	+	-	UU	A00126:224:H7HMWDSX3:3:1109:30852:3178297Test_seq16608460151M=166403470TCAGGAGTAGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTGGGTCTCTGGTTCATGTGGCCCTTTCTATCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFF,FF,FFF,FFFFFFFFF,:FF,FFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:8T77A26G26G10MC:Z:151MAS:i:131XS:i:71Yt:Z:UU	A00126:224:H7HMWDSX3:3:1109:30852:31782145Test_seq16640360151M=166084-470CAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCF:FFFFFF:FFFFFFFFF::FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFF,FF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:102T48MC:Z:151MAS:i:146XS:i:40Yt:Z:UU
@@ -353,7 +353,7 @@
 A00126:224:H7HMWDSX3:3:1110:12020:6057	!	0	Test_seq	171156	-	+	NR	A00126:224:H7HMWDSX3:3:1110:12020:605781Test_seq17135060121M30S=171156-315GGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGATAGAGTCCCACATGATTCTTATGAGGATGGTGTTTCATTCTCTTGTACTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:50G15T54MC:Z:151MAS:i:111XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1110:12020:6057161Test_seq17115660151M=171350315CTGAACTTATTCTTATTGATTCTCCCTTGAAGGAAACCTTTCTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGGTTCGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:121M30SAS:i:151XS:i:35Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1110:15130:6151	!	0	!	0	-	-	MM	A00126:224:H7HMWDSX3:3:1110:15130:615165Test_seq333515062M=38398650472CCTTTGCATAGTCAATAAAACACAGGTAAACATCCTTCTGGTATTCTCTGCCTTCAGCCAGGFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFF,F::FFFFFFFFF:FF,F,:,FNM:i:1MD:Z:51T10MC:Z:62MAS:i:57XS:i:57XA:Z:Test_seq,-383986,62M,1;Test_seq,+15151,62M,2;Test_seq,+316240,19M2I41M,3;Yt:Z:MM	A00126:224:H7HMWDSX3:3:1110:15130:6151129Test_seq383986062M=333515-50472CCTGGCTGAAGGCAGAGAATACCAGAAGGATGTTTACCTGTGTTTTATTGACTATGCAAAGGFFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FF:FFFFF,FFFF::FFFFFF,F:FFF:F:FFNM:i:1MD:Z:10A51MC:Z:62MAS:i:57XS:i:57XA:Z:Test_seq,-333515,62M,1;Test_seq,-15151,62M,2;Test_seq,-316240,19M2I41M,3;Test_seq,+449331,62M,4;Yt:Z:MM
 A00126:224:H7HMWDSX3:3:1110:18936:8046	Test_seq	168392	Test_seq	168721	+	-	UU	A00126:224:H7HMWDSX3:3:1110:18936:8046161Test_seq16839260151M=168571330TCCGGATACCCTTTATTTCTTTGTCTAGCCTAATTGCCCTGGCTAGGACTTCAAGCATGATGTTGAATAAGAGCGGTGATAAGGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATGTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFFFFNM:i:6MD:Z:2T52T1C24A20T44A2MC:Z:151MAS:i:125XS:i:73Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:18936:804681Test_seq16857160151M=168392-330ATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAAFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFNM:i:1MD:Z:145T5MC:Z:151MAS:i:146XS:i:73Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1110:19090:8406	!	0	Test_seq	169404	-	-	MR	A00126:224:H7HMWDSX3:3:1110:19090:8406177Test_seq523240127S24M=169254117058AGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGATGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAACTCTCTGTCTCTTTATTGGTGCAFFFFFFFFF:F,,FFFFFF:FF::F,F,FFFF:FFFF:F:FFFFFFF,FFFF:FF,F:FFF,FFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:24SA:Z:Test_seq,168994,+,22S129M,60,1;XA:Z:Test_seq,-154647,127S24M,0;Test_seq,+255573,24M127S,0;Test_seq,+38332,24M127S,0;Test_seq,-170380,127S24M,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1110:19090:84062209Test_seq1689946022H129M=169254411GTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTFFFFFFFFFFFFFFFFFFF,FFFFF:FF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFF,FFF:F,FF:FFFF,FFFFFFF:F:FFFF:FFFF,F,F::FF:FFFFFF,,F:FFFFFFFFFNM:i:1MD:Z:44G84MC:Z:151MAS:i:124XS:i:49SA:Z:Test_seq,52324,-,127S24M,0,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1110:19090:8406113Test_seq16925460151M=52324-117058TGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFF:FFFFFFF:FFFFFFFF:FFFFF:F:FFFF:FFFFFFFFFF:FF:FFFFFFFFF:FFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFF:FF,F:FFNM:i:1MD:Z:14T136MC:Z:127S24MAS:i:146XS:i:82Yt:Z:MR
+A00126:224:H7HMWDSX3:3:1110:19090:8406	!	0	Test_seq	169404	-	-	MR	A00126:224:H7HMWDSX3:3:1110:19090:84062209Test_seq1689946022H129M=169254411GTTAAGTCTTCTCTGAAGGTTTGGTAGAACTCTGCAGTGAAGCCATCTGGGCCAGGACTTTTTTTTGTTGGAAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTFFFFFFFFFFFFFFFFFFF,FFFFF:FF:F,FFFFFFFFFFFFFFFFFFFFFFFFF:FF,FFFFFFF,FFF:F,FF:FFFF,FFFFFFF:F:FFFF:FFFF,F,F::FF:FFFFFF,,F:FFFFFFFFFNM:i:1MD:Z:44G84MC:Z:151MAS:i:124XS:i:49SA:Z:Test_seq,52324,-,127S24M,0,0;Yt:Z:MRNEXT_SAMA00126:224:H7HMWDSX3:3:1110:19090:8406177Test_seq523240127S24M=169254117058AGAACAACTAAATAGACCCATAACAAAAAAAGAGATTGAAACGGTAATCAAAAAACTTCCAACAAAAAAAAGTCCTGGCCCAGATGGCTTCACTGCAGAGTTCTACCAAACCTTCAGAGAAGACTTAACTCTCTGTCTCTTTATTGGTGCAFFFFFFFFF:F,,FFFFFF:FF::F,F,FFFF:FFFF:F:FFFFFFF,FFFF:FF,F:FFF,FFFFFFF,FF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FF:FFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:,NM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:24SA:Z:Test_seq,168994,+,22S129M,60,1;XA:Z:Test_seq,-154647,127S24M,0;Test_seq,+255573,24M127S,0;Test_seq,+38332,24M127S,0;Test_seq,-170380,127S24M,0;Yt:Z:MR	A00126:224:H7HMWDSX3:3:1110:19090:8406113Test_seq16925460151M=52324-117058TGTTGTGATGTGGTCCTTCTCGTTTCTTATTCGGGTTATTTGTTTCCTTTCCTGTTTTTCTTTAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:F:FFF:FFFFFFF:FFFFFFFF:FFFFF:F:FFFF:FFFFFFFFFF:FF:FFFFFFFFF:FFFFFFFFFFF,:FFFFFFFFFFFFFFFFFFFFFFF:FF,F:FFNM:i:1MD:Z:14T136MC:Z:127S24MAS:i:146XS:i:82Yt:Z:MR
 A00126:224:H7HMWDSX3:3:1110:18765:8531	Test_seq	169027	Test_seq	169502	+	-	UU	A00126:224:H7HMWDSX3:3:1110:18765:853197Test_seq16902760151M=169352476GCAGTGAAGCCGTCCGGGCCAGGACTTTTTTTTGTTGGGAGTTTTTTGATTACCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGTTCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F,FFFFF,FFF:FFFFFFFFFF:FFF:FFFFFF::FFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFF,F:FF:FF,FFFFF:FF,:FNM:i:5MD:Z:14T23A57A37G1A14MC:Z:151MAS:i:126XS:i:61Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:18765:8531145Test_seq16935260151M=169027-476TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGFFFFFFFFF:FFF,FFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:115G35MC:Z:151MAS:i:146XS:i:101Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1110:23927:10676	Test_seq	166048	Test_seq	166495	+	-	UU	A00126:224:H7HMWDSX3:3:1110:23927:10676161Test_seq16604860151M=166345448AATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCTTTTCTGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:143C7MC:Z:151MAS:i:146XS:i:45Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:23927:1067681Test_seq16634560151M=166048-448TTTTCATAATAGAATTATTTTGCCCATTGACTTAGAAGTCCCCTCAAACCAAGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAGGCATTCATTTTATCCTGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:6MD:Z:25G14T10T38A2T12C44MC:Z:151MAS:i:121XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1110:29161:13416	Test_seq	169352	Test_seq	169778	+	-	UU	A00126:224:H7HMWDSX3:3:1110:29161:1341697Test_seq16935260151M=169628427TTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGGTTCTTTTGTTGCTCACTTTCTATTTGTTCAAGTTGFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFF:FFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF,FFFFFFFFFFFF,FFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:66Yt:Z:UU	A00126:224:H7HMWDSX3:3:1110:29161:13416145Test_seq16962860151M=169352-427CGTTGCTTTCTAAGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATGTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATTTCTAGCTTCATTGCCTTGTGGTCTGAFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:0T34G54T28C31MC:Z:151MAS:i:135XS:i:62Yt:Z:UU
@@ -393,7 +393,7 @@
 A00126:224:H7HMWDSX3:3:1111:27254:10113	!	0	Test_seq	169321	-	+	NR	A00126:224:H7HMWDSX3:3:1111:27254:10113145Test_seq16962860119M32S=169321-426CGTTGCTTTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTCGTTTTTCTGTTATTGATCGATCTCTTTATTTGCACTGCTTTAGGFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFNM:i:4MD:Z:0T11A22G70A12MC:Z:151MAS:i:103XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1111:27254:1011397Test_seq16932160151M=169628426AGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATTCTFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFF,:FFFF:F,FFFF,:FF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFNM:i:1MD:Z:146G4MC:Z:119M32SAS:i:146XS:i:112Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1111:22200:10222	Test_seq	117403	Test_seq	117847	+	-	UU	A00126:224:H7HMWDSX3:3:1111:22200:10222161Test_seq11740360151M=117697445AATCCACTTAAGTATTTGAATGTTATTTCAACCTCTTTCTATGAGGAAGAGCTTTGGGAAAAAATAAGTAGAAAGTGAAAATATCGATAGTGATTAATGAGTGATGGCTGGCTTTGACTGCTGTAGGAGTAGGTAGGACAACCGCTACAAAFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFF,FF:FFFF,FFFFFFFFFFFFFFF,F:FFFFFFFFFFFF:FFFFF:FFFFFFFFFFF,FF:FFFFFFFF:F:FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:148G2MC:Z:151MAS:i:148XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:22200:1022281Test_seq11769760151M=117403-445TCCTCTTTCTGTGGGATGGACAAGGATGGAGCCTAGGGCCCCATCCCTGTCACATTTCTGCACAGTCCTCAGACACTTGCAGAAAGTGTTGCCGGTTTTTAGCACATCTTTCTCTGGAGATGAAACTGGCTCAGCACCAACCAGAGGGCACFFFFFF,FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:17499:11537	Test_seq	171298	Test_seq	171659	+	-	UU	A00126:224:H7HMWDSX3:3:1111:17499:1153797Test_seq17129860151M=171509362TGGGGTTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTCTGTCAGGAGTTCTTCAACTATTTTCTCTGTGTTTTCTGTCCCCCCTCCCTGTTCTGGGACTCCAATCACTCGCAAGTTATCCTTCTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FFF:F:FFFFFF,FF:F,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF:FFFF,F:FFFFFFFFFF:NM:i:3MD:Z:62G39G15T32MC:Z:151MAS:i:136XS:i:61Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:17499:11537145Test_seq17150960151M=171298-362TTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCTAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:47Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1111:19994:11788	Test_seq	20289	Test_seq	168429	-	+	UR	A00126:224:H7HMWDSX3:3:1111:19994:11788145Test_seq202637124S27M=168429148141TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCTTTTCTTTCTTTTTAATGACCTCTTGCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:6G20MC:Z:151MAS:i:22XS:i:20SA:Z:Test_seq,168657,-,120M31S,60,3;XA:Z:Test_seq,-55966,131S20M,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1111:19994:117882193Test_seq16865760120M31H=168429-348TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:3MD:Z:18C30T9T60MC:Z:151MAS:i:105XS:i:50SA:Z:Test_seq,20263,-,124S27M,7,1;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1111:19994:1178897Test_seq16842960151M=20263-148141CCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFF:FF,FFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,NM:i:1MD:Z:66T84MC:Z:124S27MAS:i:146XS:i:78Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1111:19994:11788	Test_seq	20289	Test_seq	168429	-	+	UR	A00126:224:H7HMWDSX3:3:1111:19994:117882193Test_seq16865760120M31H=168429-348TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:3MD:Z:18C30T9T60MC:Z:151MAS:i:105XS:i:50SA:Z:Test_seq,20263,-,124S27M,7,1;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1111:19994:11788145Test_seq202637124S27M=168429148141TGCATCTATTGATAAGATGATGTGGTTTTTATCTTTTGTTTTATTTATGAGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCGATCTTTTCTTTCTTTTTAATGACCTCTTGCF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:1MD:Z:6G20MC:Z:151MAS:i:22XS:i:20SA:Z:Test_seq,168657,-,120M31S,60,3;XA:Z:Test_seq,-55966,131S20M,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1111:19994:1178897Test_seq16842960151M=20263-148141CCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFF:FF,FFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF,F,F:FFFFFF,NM:i:1MD:Z:66T84MC:Z:124S27MAS:i:146XS:i:78Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1111:20690:11929	!	0	Test_seq	167597	-	-	NR	A00126:224:H7HMWDSX3:3:1111:20690:1192997Test_seq1672982124S50M1I76M=167447300TTTGAAGGCTTGTCTTGTGAATTTAATCATGTAGATTTTAGAGATCAGGCGCTGGTCTGAGATGTCATAGCTGACATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF,FFFNM:i:3MD:Z:25A44A55MC:Z:151MAS:i:109XS:i:99XA:Z:Test_seq,-213611,74M1I52M24S,5;Test_seq,+400719,24S50M1I76M,7;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1111:20690:11929145Test_seq16744760151M=167298-300TCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:24S50M1I76MAS:i:151XS:i:101Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1111:24795:12649	Test_seq	168660	Test_seq	212799	-	-	UU	A00126:224:H7HMWDSX3:3:1111:24795:12649113Test_seq16851060151M=21264944140TGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGGACTTTGTCAAATGCCTTTTCTGCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:126A24MC:Z:151MAS:i:146XS:i:78Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:24795:12649177Test_seq21264925151M=168510-44140GGCAAGGAAGAAGTAAAGTTATCACCATTTGCAGATGACATGATTATATACACAGAAAACCCTAAGGAATCCAACAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:10MD:Z:8G8A5T1T18C0T17T8T0C51G25MC:Z:151MAS:i:101XS:i:86XA:Z:Test_seq,+168239,151M,13;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:8802:14732	Test_seq	170230	Test_seq	170661	+	-	UU	A00126:224:H7HMWDSX3:3:1111:8802:14732161Test_seq17023060151M=170511432AATATTGCTACTCCTCTTCTTTTTTGCTTATTATTTGCTTGATATATCTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGAFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFNM:i:1MD:Z:47T103MC:Z:151MAS:i:146XS:i:72Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:8802:1473281Test_seq17051160151M=170230-432TTTTGTGCTGAGACGTTTTTCTTAGTAGATTGTGAGATCCTCATTTTCATAATGTTTAACTTTATGTTTGTTGAGTTGTTACGTTTTTCTTGAGTTATGGAATCGATATTCCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:76C26T47MC:Z:151MAS:i:141XS:i:37Yt:Z:UU
@@ -424,7 +424,7 @@
 A00126:224:H7HMWDSX3:3:1111:1570:34773	Test_seq	72280	Test_seq	72638	+	-	UU	A00126:224:H7HMWDSX3:3:1111:1570:3477397Test_seq7228060151M=72488359ATCAGTCTGTTTTGAGATAGAAAAGAGATTAAACAAGGAGATTAAAGAAGCAGCGATGGAGGAAGAGAGATGCAAGGCCGCATGAAGGTCGCCCAAGAGCAGAAGCTCAAAAGAGGCTAAGGTCTTCCTCCAGAGCTGAGATAGAAAGCTTFFFF:FFFFFFFFFFFFFF:FFFFF::FFFFF:FFF:FFFFFFFF:FFFFFFFFFF:FF:FFFF::FF:FFF:FFFF,FFFFFFFFFFFF::FFFF::FFFF:FFFFFFF,F:,FF:FF:FFFFFFFF:FFFFFF:FFFFFFFFFF:FF:FNM:i:1MD:Z:137A13MC:Z:151MAS:i:146XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:1570:34773145Test_seq7248860151M=72280-359AAATTTGTATGTTAAAACCGCCTACTTGTGGTATTTCTGTTATAGAACACTGGATAACTAGGACAGTTGCCGTGAAGGGGCAGCCGACTCGATGGCAGCCAAAAACAACAACAACAGCAGTGAAACACAGGTAGTGTTTCTTGGATTCATTF:FFFFFFF:,F:FFFFFF:,FFFFFF,F,F:,FF:FFFFFFFFFFFFFFF,FFFFFFFF:FFFF:FFFFFFFF:FFFFFFF:F,FFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFF:F:::FF:FFFFFF:NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1111:14064:36808	Test_seq	167148	Test_seq	401034	+	-	UU	A00126:224:H7HMWDSX3:3:1111:14064:3680897Test_seq16714842151M=400900233887AATTTGCATTTCTCTAATGGCTAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF::F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFF,,F:FFF,,FFF:FFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFNM:i:1MD:Z:95T55MC:Z:135M16SAS:i:146XS:i:126XA:Z:Test_seq,-213736,151M,5;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1111:14064:36808145Test_seq4009004135M16S=167148-233887TTATCGGGTTTCTCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATACCTTGTATTAGGGCTCCTAGGGTTGTCCCAATTTTTTCTTCCATGATCTTTATCATTTTAGTCTTTATGTTTAGGTCTTTGATCGATCCCTTCCCCACTC,FF:FFFF,FFFFFFFF,FFFF:FF:FF,F:FFFFFFFFF:FFFFF:FF:FFF:FFFFF:FFFFFFFFFFFFFFFF,FFFFFFFFFFFF:FFF:FFFFFFFF:FFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFF:FFFF,F:FNM:i:7MD:Z:4T0T11G32G19A0C8T54MC:Z:151MAS:i:104XS:i:100XA:Z:Test_seq,-167437,135M16S,7;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:23149:2597	Test_seq	169079	Test_seq	169468	+	-	UU	A00126:224:H7HMWDSX3:3:1112:23149:259797Test_seq1690793739M4I108M=169318390CCGTTTCAATCTCTTTTTTTGTTATGGGTCTATTTAGTTGATCGATCTGCTTCTGAATGTGTTAGTTTAGGTAGGTAGTGTTTTTCAAGGAATTCATCCATTTCTTCTAGGTTTTCAAATTTGTTAGAGTACAATTTTTCATAATAATCTGFFF:FFF:FFFFF,FFFFFFFFFFFFFFF:F:FFFFFFFFFFF,FFFFFFF:FF:F:F:F::FFFFFF:FFFFF:FFFFFFF:FFFFF,FFF,F:FFFFFFFFFFFFFFF,FF:F:FFFFFFF:FF,FF,FFFFFF,:,,F:F:,F:F,:FNM:i:12MD:Z:40T3A37G1A37C11A1G2G7MC:Z:151MAS:i:99XS:i:76Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:23149:2597145Test_seq16931860151M=169079-390GTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATTTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGATFFFFFFFFFFFFFFFFFF,FFFF:FFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:1MD:Z:149G1MC:Z:39M4I108MAS:i:149XS:i:112Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1112:10447:5243	Test_seq	168427	Test_seq	168802	+	-	RU	A00126:224:H7HMWDSX3:3:1112:10447:5243161Test_seq16842760151M=168773376GCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCAGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:68T82MC:Z:121S30MAS:i:146XS:i:43Yt:Z:RU	A00126:224:H7HMWDSX3:3:1112:10447:524381Test_seq16877360121S30M=168427-376TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCGATCAGGGTGAATTATTTTTTTGATGTGTTFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,168555,-,121M30S,60,1;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1112:10447:52432129Test_seq16855560121M30H=168427-249TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A95MC:Z:151MAS:i:116XS:i:70SA:Z:Test_seq,168773,-,121S30M,60,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1112:10447:5243	Test_seq	168427	Test_seq	168802	+	-	RU	A00126:224:H7HMWDSX3:3:1112:10447:5243161Test_seq16842760151M=168773376GCCCTGGCTAGGACTTCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCAGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:68T82MC:Z:121S30MAS:i:146XS:i:43Yt:Z:RU	A00126:224:H7HMWDSX3:3:1112:10447:52432129Test_seq16855560121M30H=168427-249TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:25A95MC:Z:151MAS:i:116XS:i:70SA:Z:Test_seq,168773,-,121S30M,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1112:10447:524381Test_seq16877360121S30M=168427-376TGCATAGATGCCCTTTATTATGTTGCGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATGGGTGTTGAACTTTGTCAAATGCCTTTTCTGCATCTATTGATAAGATCGATCAGGGTGAATTATTTTTTTGATGTGTTFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFNM:i:0MD:Z:30MC:Z:151MAS:i:30XS:i:0SA:Z:Test_seq,168555,-,121M30S,60,1;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1112:21884:7012	Test_seq	167977	Test_seq	168320	+	-	UU	A00126:224:H7HMWDSX3:3:1112:21884:701297Test_seq16797726151M=168170344AGTGCGTTAAATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGCAGTAGAGCTTTGTAGTTTTCTTTGTATAGGTCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFFFNM:i:8MD:Z:9T64G8C32T2C4T3A13G8MC:Z:151MAS:i:111XS:i:96XA:Z:Test_seq,-212911,141M10S,9;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:21884:7012145Test_seq16817031151M=167977-344TGGGGGCTACTGTGAATGGTATTGATTTGGTTATTTCCTCTTCGGTGTTCTTTTTGTTGATGTAGAGGAATCCAAGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTAGTTTCAGTAGTTTTCTGGAGGAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:101T49MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,+212718,151M,4;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:16595:8124	Test_seq	170144	Test_seq	170457	+	-	UU	A00126:224:H7HMWDSX3:3:1112:16595:8124161Test_seq1701446016S135M=170307314TTTTATGTTATTGATCGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGTGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTGTTTGCTTGATATATTTFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:F:FFFFFFFFF:FFFFFF:FFFFFFFF:FFFFFFFFF:FFF,FFFFFFFF:FFFFFF:F,FFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFF,FNM:i:3MD:Z:51C0A65A16MC:Z:151MAS:i:120XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:16595:812481Test_seq17030760151M=170144-314TGTGTCTCTAAGTCTACGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:16A134MC:Z:16S135MAS:i:146XS:i:48Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:9209:9361	Test_seq	212679	Test_seq	212953	+	-	UU	A00126:224:H7HMWDSX3:3:1112:9209:936197Test_seq21267960151M=212803275GCAGATGACATGATCTTATACACAGAAAACCCTAAGGAATCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCAAAGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:33T117MC:Z:151MAS:i:146XS:i:110Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:9209:9361145Test_seq21280358151M=212679-275TCTACATCAACAAAAAGAACATCAAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGAGAAAATACTTAGGAATAAATCTTACCAAAGATGTAAAAGACCTATACAAAGAAAACTACAAAGCACTACTACAAGAAATFFFFFFF:FFFFFFFFFFFFFFFFFFFF:::FFFF,F,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFNM:i:5MD:Z:34A37T54T7T14C0MC:Z:151MAS:i:130XS:i:100Yt:Z:UU
@@ -436,7 +436,7 @@
 A00126:224:H7HMWDSX3:3:1112:5927:14763	Test_seq	366670	Test_seq	367055	+	-	UU	A00126:224:H7HMWDSX3:3:1112:5927:1476397Test_seq36667060151M=366905386AGTGTTAAAAAGCAAAGATGTTACCTTGAAGACTAAGGTGTGCCTGACCGAAAAAAAGACCGAAGCCATGGTATTTTCAATTGCATCATATGCATGTGAAAGCTGGACAATGAATGAGAAAGGCCAAAGAAGAATTGATGTTTTTGAATTAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:50Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:5927:14763145Test_seq36690560151M=366670-386TCCTTAGAAGCAAGATTGTCAAGACTTTGATGTACTTTGGGCATGTTATCAGGAAGGATTCATCCCTAGAGAAGGACACCATGCTTGGTAAAATAGAAAAAAACCAAAACCAAACCCACTGCCGTCAAGTGATTCCGACTCATAGCGATCTF:FFFFFFF::FF:FFFFFFFF:F,FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFF,FFF,FFFF,FFFFFFFF,FFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:61G89MC:Z:151MAS:i:146XS:i:22Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:9254:15922	Test_seq	260973	Test_seq	261025	+	-	UU	A00126:224:H7HMWDSX3:3:1112:9254:1592297Test_seq260973853M=26097353GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCTGTTGTAGAAGGGCACFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFF,FF:FFFF,,,FFFFFNM:i:3MD:Z:37G7C0T6MC:Z:53MAS:i:40XS:i:36XA:Z:Test_seq,+347640,45M8S,2;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:9254:15922145Test_seq2609733453M=260973-53GGGGTTCTCTGTTGTGTTCCCTGTCAGGGCAGTCATCGGTTGTAGCTGGGCACFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:53MC:Z:53MAS:i:53XS:i:43XA:Z:Test_seq,-150325,53M,2;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:32723:16391	!	0	Test_seq	171881	-	-	NR	A00126:224:H7HMWDSX3:3:1112:32723:16391161Test_seq1716266027S124M=171731256TTCAATAAAAACCCAAATGAAATCGATTTAATCTTTTGGATTTCTACATGCTGTCTCTCTATGGATTCTTGCAACTTGTTAATTTTTCCACTATGTTCTTGAATAATCTTTTTGAGTTCTTCAACAGTTTTATCAGTGTGTTCCTTGGCTTFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFF:,FFFFFF,F:,FFFFF:FFFFFFFF:F,FFFFFFFFF:FF,FF,FFFFFF,FFFFFFFFFF,,:F:,FFFFF,F:FF:,,F,FFFNM:i:4MD:Z:50A14C13C11T32MC:Z:151MAS:i:104XS:i:71Yt:Z:NR	A00126:224:H7HMWDSX3:3:1112:32723:1639181Test_seq17173160151M=171626-256TCAGTGTGTTCCTTGGCTTTTTCTGCATTTATCCTAATTTCATTTGTGATATCTTTAAGCATTCTGTAAATTAGTTTTTTATATTCTGTATCTGATAATTCCAGGATTGTATCTTCATTTGGGAAAGATTTTGATTCTTTTGTTTGGGGGGF,FF:FFF,FFF:FFFFFFFFFFFFFFF:F:FFFF,FFFFF:FFFFFFFFFFFFFFFFFFF,FFFFFFF,FFFFFFF:F:F:,:FFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFF:,FFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFNM:i:1MD:Z:27G123MC:Z:27S124MAS:i:146XS:i:25Yt:Z:NR
-A00126:224:H7HMWDSX3:3:1112:27633:17722	Test_seq	385678	Test_seq	388748	-	-	UR	A00126:224:H7HMWDSX3:3:1112:27633:17722113Test_seq38565530127S24M=3885983071AAGTGCGTCAGAAGAAAGTCTGCAAAGCATTTCAGAGAGCACTCAAAGAGAAGCCTTTTAAGGAAAACTCTGAACAAAAATTATCCTCATAAGATAATTCTCTCTTCAACACTCCTCATTCTGGAGTAATCACAGACTGTTCACACATTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,388349,+,24S127M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1112:27633:177222145Test_seq3883496024H127M=388598400ACTCCAGAATGAGGAGTGTTGAAGAGAGAATTATCTTATGAGGATAATTTTTGTTCAGAGTTTTCCTTAAAAGGCTTCTCTTTGAGTGCTCTCTGAAATGCTTTGCAGACTTTCTTCTGACGCACTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0SA:Z:Test_seq,385655,-,127S24M,30,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1112:27633:17722177Test_seq38859860151M=385655-3071AGATTGGCCATGAATGGGGTGATAAGAATAGGGAGAAGCACATACCAGTAGACCAGACTTCCTGTGCACAAGCCTTTGAATCCTGCTTCTCGCTTCTGAAATAGCATTCATCTTAGGGCTGGGATCATTTATTCTCTCTGGAATTAAGCAAFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1112:27633:17722	Test_seq	385678	Test_seq	388748	-	-	UR	A00126:224:H7HMWDSX3:3:1112:27633:177222145Test_seq3883496024H127M=388598400ACTCCAGAATGAGGAGTGTTGAAGAGAGAATTATCTTATGAGGATAATTTTTGTTCAGAGTTTTCCTTAAAAGGCTTCTCTTTGAGTGCTCTCTGAAATGCTTTGCAGACTTTCTTCTGACGCACTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:127MC:Z:151MAS:i:127XS:i:0SA:Z:Test_seq,385655,-,127S24M,30,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1112:27633:17722113Test_seq38565530127S24M=3885983071AAGTGCGTCAGAAGAAAGTCTGCAAAGCATTTCAGAGAGCACTCAAAGAGAAGCCTTTTAAGGAAAACTCTGAACAAAAATTATCCTCATAAGATAATTCTCTCTTCAACACTCCTCATTCTGGAGTAATCACAGACTGTTCACACATTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFNM:i:0MD:Z:24MC:Z:151MAS:i:24XS:i:0SA:Z:Test_seq,388349,+,24S127M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1112:27633:17722177Test_seq38859860151M=385655-3071AGATTGGCCATGAATGGGGTGATAAGAATAGGGAGAAGCACATACCAGTAGACCAGACTTCCTGTGCACAAGCCTTTGAATCCTGCTTCTCGCTTCTGAAATAGCATTCATCTTAGGGCTGGGATCATTTATTCTCTCTGGAATTAAGCAAFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:127S24MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1112:25825:18098	!	0	Test_seq	171611	-	-	NR	A00126:224:H7HMWDSX3:3:1112:25825:18098161Test_seq1711746033S118M=171461438TATATTGAGTATATCATCATTTGACCTCAAAGAATTCTCCCTTGAAGGAAACCTTTTTTTTCTCCCTGGCTGCTTTTAAAATTTTCTGTTTGTCTTTGGTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFF:FFFFFFF:FFFFFFFFFFFFF,F:FFFFFFFFFNM:i:1MD:Z:23C94MC:Z:151MAS:i:113XS:i:35Yt:Z:NR	A00126:224:H7HMWDSX3:3:1112:25825:1809881Test_seq17146160151M=171174-438ATGATTCTTAGGGTTTCTTCATTTTTTTTAATTCTTTTATCTGATTTTTTTTCAGCTATGTTGGAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGCTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:122T28MC:Z:33S118MAS:i:146XS:i:39Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1112:9362:18740	Test_seq	170285	Test_seq	170742	+	-	UU	A00126:224:H7HMWDSX3:3:1112:9362:18740161Test_seq17028560151M=170592458ATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCCTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGAFFF,FFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF:,FF::FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF,FFFF:FFFF:FFFFFFFFF:FFFFF:FFFF,F::FFF,F:FFFFFF,F,:FFFFFFFFF,FFF,FFFFFNM:i:1MD:Z:107T43MC:Z:151MAS:i:146XS:i:82Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:9362:1874081Test_seq17059260151M=170285-458CTTTTTTCTTGAGTTATGGAATTGATATTCCTTTTTGTGGTTACCTTTTTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCCTTCTATTTCGCCTTGTATCACTCTCCATCTGGCAGTTCGATGCCTCCTATATTTAGTCCCFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFF:FF,FFFFFFFF:FFFF,FFFFF:F,FFFFFF:FFFFF:FFFF:FFFFFFF,FFFFFFFFFNM:i:8MD:Z:1G45A42G2G3A2A28A9G11MC:Z:151MAS:i:114XS:i:24Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1112:31421:19304	Test_seq	236629	Test_seq	237126	+	-	UU	A00126:224:H7HMWDSX3:3:1112:31421:1930497Test_seq23662960151M=236976498AAGAAAAATAAAAGTAAATTCCACAGTAAGGTGAACAATTAAGATGAGATTACCAACTGAGATTACCCATTTCAGAAAGAAGTATTCTAAGTAAAAGGTAATCAAAAGTTGGCTTTTCTACTTTCAATAGAGGATTCACACATAGTCATTGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1112:31421:19304145Test_seq23697660151M=236629-498TAAAATACATGCAGCAGAAGGTTAGTACGTAAAGACTAATTCAAACTATGCACTGATGTGTTGTTGTTAGCTGCTGTCAGGTTGGCCCTGACTCATGGTGATGCCACGCACAATGGAACAAAAGGCTGCCAGGTCCTGTGTTATATCCCATFFF:::FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:28Yt:Z:UU
@@ -470,7 +470,7 @@
 A00126:224:H7HMWDSX3:3:1113:4526:3975	Test_seq	168585	Test_seq	212883	-	-	UU	A00126:224:H7HMWDSX3:3:1113:4526:3975113Test_seq16843560151M=21273344299TAGGACTCCAAGTACGATGTTGAATAAGAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCTGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:7T143MC:Z:151MAS:i:146XS:i:78Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:4526:3975177Test_seq21273320151M=168435-44299CTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAAATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATCACCAAATCAATACCATTCACAGTAGCCCCCAAGAAGATAAAATACTF:FF:FFFFFFF::FFFFFFF,:FFF:,F,FFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFFF,:FFFF,F:FFFFFFFFFF:FF:FFFFFF:F,FFFFFFFFF:::FFFFF,FFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFF:FFFFFNM:i:3MD:Z:41G51A10A46MC:Z:151MAS:i:136XS:i:126XA:Z:Test_seq,+168155,151M,5;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1113:5565:7560	!	0	Test_seq	166317	-	+	NR	A00126:224:H7HMWDSX3:3:1113:5565:756081Test_seq16643460120M31S=166317-237AAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTTTAGTCCAGTCCAATTAGGCTGACCTTCCTTGTATTGTGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCTAAAATTCAAGTCATATCTTGAAATTCACTA:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:120MC:Z:151MAS:i:120XS:i:30Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:5565:7560161Test_seq16631760151M=166434237GCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTCCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCTTTGCTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFF:FFFFNM:i:1MD:Z:49G101MC:Z:120M31SAS:i:146XS:i:32Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:12644:7701	Test_seq	424202	Test_seq	424540	+	-	UU	A00126:224:H7HMWDSX3:3:1113:12644:770197Test_seq42420260151M=424407339TGGGGCAGAAGACTGCTGTTTTTCACACTTAGAGAATAAGACCTTTTCAAACTCAACATGTCCACTTGGATGTCTAATAGACATCTTAAACTTAATATATTTAAAACAATATTTGGCCCCAAAGCTGCCCTATTTTGTGTCAGCTGATGGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF,FFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:134M17SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:12644:7701145Test_seq42440760134M17S=424202-339ATATCCAATCCTTCAGGAAATCCTTTAGAATATCTCCAGACTCTGACCATCTTGCGCTTCCTCTGCTGTCACCATCCTGGGTCAAGCCCATCATCTCTCACCTGAACTCCTGCTCATAATCTCCTCCCAGATCTAAACTACACAGTATCTAFF:FFFFFFFFFFFFFF:FFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF:FF:FFFFFFFFFNM:i:0MD:Z:134MC:Z:151MAS:i:134XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1113:13765:8735	Test_seq	167568	Test_seq	328153	+	+	RU	A00126:224:H7HMWDSX3:3:1113:13765:8735129Test_seq16756832151M=328153160586GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:2S25M124SAS:i:151XS:i:136XA:Z:Test_seq,-213320,140M2I9M,3;Yt:Z:RU	A00126:224:H7HMWDSX3:3:1113:13765:873565Test_seq328153192S25M124S=167568-160586AAGGAGCCCTGGTGGTGCAGTGGTTAGATCCAAATTCCAATGTCATATTTTAAGGGGATAGAGAAACAAATCACTAATTTCATATGGAAGGGAAAGAACCCCCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCAFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF,FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFNM:i:0MD:Z:25MC:Z:151MAS:i:25XS:i:21SA:Z:Test_seq,167849,-,124M27S,60,1;XA:Z:Test_seq,+141607,26M125S,1;Test_seq,+432045,26M125S,1;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1113:13765:87352129Test_seq16784960124M27H=167568-405TGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGAATTTGGATFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF,FFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:115T8MC:Z:151MAS:i:119XS:i:75SA:Z:Test_seq,328153,+,2S25M124S,19,0;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1113:13765:8735	Test_seq	167568	Test_seq	328153	+	+	RU	A00126:224:H7HMWDSX3:3:1113:13765:8735129Test_seq16756832151M=328153160586GATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGTTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATTGACTGACACTGGTCCTTTGTCAAATATCAGCFFFFFFFFFFFFF,FFFF,FFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFF,FFFFFFF:FFFFFFFFFFFF::FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:2S25M124SAS:i:151XS:i:136XA:Z:Test_seq,-213320,140M2I9M,3;Yt:Z:RU	A00126:224:H7HMWDSX3:3:1113:13765:87352129Test_seq16784960124M27H=167568-405TGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGAATTTGGATFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFF:FFF,FFFFFFFF:FFFFFFFFFFFNM:i:1MD:Z:115T8MC:Z:151MAS:i:119XS:i:75SA:Z:Test_seq,328153,+,2S25M124S,19,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1113:13765:873565Test_seq328153192S25M124S=167568-160586AAGGAGCCCTGGTGGTGCAGTGGTTAGATCCAAATTCCAATGTCATATTTTAAGGGGATAGAGAAACAAATCACTAATTTCATATGGAAGGGAAAGAACCCCCGGATAAGCAAAGCATTACTGAAAAAGAAGAAGAAAGTGGGAGGCCTCAFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFF,FFF:FFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFNM:i:0MD:Z:25MC:Z:151MAS:i:25XS:i:21SA:Z:Test_seq,167849,-,124M27S,60,1;XA:Z:Test_seq,+141607,26M125S,1;Test_seq,+432045,26M125S,1;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1113:8892:9878	!	0	Test_seq	397096	-	-	NR	A00126:224:H7HMWDSX3:3:1113:8892:9878161Test_seq3967016025S126M=396946396TTTTTTCTAGTATACCATTTTGATCAGTCTCTCCTGACATCCATTTTGATGTTTCTTTCCTGTCTTTTTAATGACCTTTTGCTTTGTTCATGTATGAGGTCCCTGATTTCATCCCACAGCTCATCTGGTCTTTGGTCATTAGTGTTCAGTGFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,::FFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:126MC:Z:151MAS:i:126XS:i:0Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:8892:987881Test_seq39694660151M=396701-396CATCTGATGGTCTATTCTGCAGTCGGCTCCTGGCCTTGTTCTGACTGATGATATTGAGCTTCTTCATTGTCTCTTTCCACAGATGTAGTTGATTCGATTCCTGTGTATTCTATCTGGTGAGGTGTGTATAGTCACAGTTTATTTTGTTGAAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:95A55MC:Z:25S126MAS:i:146XS:i:58Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:7084:10441	!	0	Test_seq	167640	-	-	NR	A00126:224:H7HMWDSX3:3:1113:7084:10441161Test_seq167316721S130M=167490325ATCTGACACATTTAAGTGATCGATCAGGCGCTGGTCTGAGATGTCATAGCTGAATATTCTTTCCCAGTCTGTAGGTGGTCTTTTTACTCTTTTGGTGAAGTCTTTAGATGAGCATAGGTGTTTGATTTTTAGGAGCTCCCAGTTATCGGGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFFFFFFFFFFF:FFFFFFFF::FFFFFFFFFFFFFFFFFFFFF:FFFNM:i:3MD:Z:7A44A73T3MC:Z:151MAS:i:116XS:i:111XA:Z:Test_seq,-213589,130M21S,4;Test_seq,+400737,21S123M7S,5;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1113:7084:1044181Test_seq16749060151M=167316-325CTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTACATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFNM:i:1MD:Z:102G48MC:Z:21S130MAS:i:146XS:i:96Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1113:3025:10614	Test_seq	385792	Test_seq	386184	+	-	UU	A00126:224:H7HMWDSX3:3:1113:3025:10614161Test_seq3857926015S136M=386034393GCCAGAAGGTTCCTTAATCCCTATCCCAAGGGATTTATCTCTAAAAAGAAATGCTGTTTTGTGCGTGGAATTCAGTCTGACTCATAGCACCCTGTATGACGAAGTAGAACTGCCCCACACGGTTTCCTAGGCTGTGATATTTACAAAAAAAFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FF:FF:FFFF,FFFFFFFFFFFFFFF,FFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:,FFFFFFFFFF:FFFFFFF,FFFNM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1113:3025:1061481Test_seq38603460151M=385792-393CGTTTCTTCTGTTTTTGCTCCTCATCTTCTTGCCTATTTGAGACATCACAATTTGTTTGTGCAATCCTGGAAATTCTCCTTGTTTCCACCCTCAACCCATCTCAGCCTGCTGCCTTCTTGTAGACCAGGGTCCCTGACTCTGGCCCATGCA:FFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFF,F:FFF:FFFFFFFFFFFFFFFFFFFFFFF:::FFF:FFFFF:FFF:FFFFFF,FFFF,FFF:FFFF::F:FF:F:FFFFFFFFFNM:i:0MD:Z:151MC:Z:15S136MAS:i:151XS:i:0Yt:Z:UU
@@ -507,9 +507,9 @@
 A00126:224:H7HMWDSX3:3:1114:11785:1485	Test_seq	170257	Test_seq	170704	+	-	UU	A00126:224:H7HMWDSX3:3:1114:11785:148597Test_seq17025760151M=170566448TTATTGTTTGCTTGATATATTTTTTTCCATCCTTTGAGTTTTAGTTTGTTTGTGTCTCTAAGTCTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCCGTGACTCTCTGTCTCTTTATTGGTGCATTTAFFFFFFFF:FFFFFFFFFFF:F:FFFF:FFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFF:FFFFFFF:F:FFFFFFFF:FFFFFFFFF,F:FFF,FFF:FFFFFFFF:FF:F:FFF:FFFFFFFFFFF:FFFFFFFFFF,FF:FFF,FNM:i:1MD:Z:5A145MC:Z:32M12I107MAS:i:146XS:i:80Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:11785:1485145Test_seq1705666032M12I107M=170257-448TTGACTTTATGTTAGTTGAGTCGTTACGTTTTTCTTGGCTTTTATCTTGAGTTATGGAGTTGTTATACCTTTTTGTGGTTACCTTATTATTTACCCCTATTTTTCTAAGTAAAAACCTAACTTGTATCGTTCTATATCGCCTTGTATGACTFFFFF:FFFFFFFFFFFFFFFF:FFFFF:FFF:FFF::F,FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:20MD:Z:2A10T32A3A3T64G6A8C3MC:Z:151MAS:i:84XS:i:37Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:14986:3239	!	0	Test_seq	333153	-	+	NR	A00126:224:H7HMWDSX3:3:1114:14986:323981Test_seq33337760124M27S=333153-348CTGCAGTATTCCCTTGTTCTGTCTGACCAACTGCCTCTTGATCTATGTAAAGGTTCCTTATGAGCACAATTAAGTGTTCTGGAATTCCCATTCTTCGCAGTGTTATCCAGAGTTTGTTATGATCGATCCAGGCAGGTAAACCTTCAGGCCA,:FFFFFF,:FFF:FF:,FFFFF:F,:F:,FFF,:FF,F:,FFFFFF:F,FFF::FFFFFFFFFFFFF:FF,FFFFFFFFFF:F,:FFFFFFF:FFFF:FFFF:F:FFFFFFFFF::FF:F:FFFFFFF,F:FFFF,FFFFFFFFFF:FFFNM:i:1MD:Z:0A123MC:Z:151MAS:i:123XS:i:62Yt:Z:NR	A00126:224:H7HMWDSX3:3:1114:14986:3239161Test_seq33315332151M=333377348GTGGTCTTTGATCTTCATTAGTAAGTGCTTCAAGTCCTCTTCACTTTCAGCAAGCAAGGTTGTGTCATCTGCATAACGCAGGTTGTTAAAGAGTCTTCCTCCAAACCTGATGCCCCGTTCTTCTTCATATAGTCCAGCTTCTCGTATTATTF:FF,FFF:FFFFFFFFFF:FFFFF:FFFFFFFFFFFFFF,FFFFFFFFF:FFF:F:FFFFFF:FFFF:FFFFFFF:F:FFFFFFFFFF:FFFFF:F,::::FFFF:FF:F,,F:FF,FFFFFF:,FFFFF:FFFFF,,:FF:FFFF,,F:NM:i:0MD:Z:151MC:Z:124M27SAS:i:151XS:i:136XA:Z:Test_seq,+315878,151M,3;Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1114:26268:3333	Test_seq	435684	Test_seq	436086	+	-	UU	A00126:224:H7HMWDSX3:3:1114:26268:3333161Test_seq43568460151M=435936403CTGAGCAATAATCACCGCCCATGGGGCAGTTCTGCTCTGCCCTATAGGGTTGCTATGAGTCGGAATCAACTCCAGGGCAATAGGGTTTTTTTGGTTTTACCATCACCTTGAATCAGAAACAACTAGACAGCAATAGCAAATAGCTAAAAGTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:31Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:26268:333381Test_seq43593660151M=435684-403TTATCGCAGTTGTAAATACCTTTTCTCTCTTTTTCAGGACAGACTGGCAAATCTTTGAATGTCTAATGTACACCTATGTATGATAATCTCTTTTCCTCCAGGAAAAAATTTCTGCCTTGAAAAAGCAAGCGAATCATATTGTCCAGCAGGCFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1114:5791:5008	Test_seq	70329	Test_seq	71380	-	-	UR	A00126:224:H7HMWDSX3:3:1114:5791:5008113Test_seq703106131S20M=712301052GAGACGTGGAGCCAACTTGGCCCAATTCAGTCAAGGTCACGTCCAATGATCACGTCACAGACACCAAATCCTCAGCCGCAAAAGCATGGCGAGAGGGCAGCATGCAGGTGGAAGCAGCCCATTCTAGGATCAATCCAGGTTCTGCCCCTCCFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,71226,+,20S131M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:5791:50082145Test_seq712266020H131M=71230155GATCCTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:0SA:Z:Test_seq,70310,-,131S20M,6,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1114:5791:5008177Test_seq7123060151M=70310-1052CTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCTCCCAGCCTCTAGGAGCTTATGAGFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:131S20MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1114:5791:5008	Test_seq	70329	Test_seq	71380	-	-	UR	A00126:224:H7HMWDSX3:3:1114:5791:50082145Test_seq712266020H131M=71230155GATCCTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFF,FFFFFFFFFFFFF:FFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:0SA:Z:Test_seq,70310,-,131S20M,6,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:5791:5008113Test_seq703106131S20M=712301052GAGACGTGGAGCCAACTTGGCCCAATTCAGTCAAGGTCACGTCCAATGATCACGTCACAGACACCAAATCCTCAGCCGCAAAAGCATGGCGAGAGGGCAGCATGCAGGTGGAAGCAGCCCATTCTAGGATCAATCCAGGTTCTGCCCCTCCFFF:FFFFFFFFFFFFF,FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,71226,+,20S131M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1114:5791:5008177Test_seq7123060151M=70310-1052CTAGAATGGGCTGCTTCCACCTGCATGCTGCCCTCTCGCCATGCTTTTGCGGCTGAGGATTTGGTGTCTGTGACGTGATCATTGGACGTGACCTTGACTGAATTGGGCCAAGTTGGCTCCACGTCTCTCCCAGCCTCTAGGAGCTTATGAGFFFFF:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:131S20MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1114:24153:6778	Test_seq	166090	Test_seq	166553	+	-	UU	A00126:224:H7HMWDSX3:3:1114:24153:677897Test_seq16609060151M=166418464GTTGTCTGTTGTGCTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCTCTTGGGTTCTTAGTTGTCGTGTGACCTTGGTGTTCTTCCTFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFF,F:F:FFFFF,FF::FFFFFFFFFFFF:FFFFFFFFFF:FF,:NM:i:1MD:Z:134G16MC:Z:136M15SAS:i:146XS:i:85Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:24153:6778145Test_seq16641860136M15S=166090-464GCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAATCTCTTTGCTTTTAGTCCAGTCCAGTTGGGCTGACCTTCCTTGTATTGAGTGTTGTCTTTCCCTTCACCCAAAGCAGTTCTTATCTACTGATTGATCGATCCACCGTCAATCFFFFFFF,FFFFFFFFFFFFFFFF:F:FF:FFFFFFFF,FFF:FFFFFF,FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFF,FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:39C23A2A20T48MC:Z:151MAS:i:116XS:i:40Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1114:29423:7827	Test_seq	15892	Test_seq	168477	-	+	UR	A00126:224:H7HMWDSX3:3:1114:29423:7827145Test_seq158736131S20M=168477152586TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCACCCTTTGAAATCTTCTGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,168685,-,126M25S,60,2;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:29423:78272193Test_seq16868560126M25H=168477-334TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:2A28T94MC:Z:151MAS:i:118XS:i:58SA:Z:Test_seq,15873,-,131S20M,6,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1114:29423:782797Test_seq16847760151M=15873-152586GCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18T132MC:Z:131S20MAS:i:146XS:i:92Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1114:29423:7827	Test_seq	15892	Test_seq	168477	-	+	UR	A00126:224:H7HMWDSX3:3:1114:29423:78272193Test_seq16868560126M25H=168477-334TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:2MD:Z:2A28T94MC:Z:151MAS:i:118XS:i:58SA:Z:Test_seq,15873,-,131S20M,6,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1114:29423:7827145Test_seq158736131S20M=168477152586TTGTCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTTTTCTGATATTAAACCAGCCTTGCATACCTGGTATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTAGTCACCCTTTGAAATCTTCTGTTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,168685,-,126M25S,60,2;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1114:29423:782797Test_seq16847760151M=15873-152586GCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAGTTTTTATCATAAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:18T132MC:Z:131S20MAS:i:146XS:i:92Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1114:22453:9032	Test_seq	414176	Test_seq	414453	+	-	UU	A00126:224:H7HMWDSX3:3:1114:22453:903297Test_seq41417660151M=414303278TGCCTCTTTGTCCACCTAGAAGCTTAGACAAGAAACCTGGAAGTCAGTCTTGATCCTTCTTCGTCCATCACCCCCACATCCAGTCGGTTATCAAAATACTTCTCAATTACTTCAACCTCTTCGCATCTCCACTGACAGCATTCTCCCCTCAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF,FF:FFFFF::FF:FF,FFFFFFFFFFF:F:FFFF,FFFFFFFFFFFFFF,,FF,FFFF:FFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:22453:9032145Test_seq41430360151M=414176-278TCCACTGACAGCATTCTCCCCTCAATCACCATCGACTCGCATTAAGCCCCATTTAGTTCCATTTCCCTTTCAATCCATCTCTTCACAGCAGCCAGGGTGATTTAAAAAAATATATAAATTACAGCACATCACTCTGGTGTGTGGCCCACTGFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:27959:9392	Test_seq	171239	Test_seq	171657	+	-	UU	A00126:224:H7HMWDSX3:3:1114:27959:939297Test_seq17123960151M=171507419GTTTTGGCAAGTTTGATGATAATATGTCTTGGTGTTTTTCTTTTTGGATCAATCTTAAATGGGATTCGATGAGCATCTTGGATAGATATCCTTTCGTCTTTCATGATGTCAGGGAAGTTTTGTGTCAGGAGTTCTTCAACTATTTTCTCTGF,,FF:FFFFFFF,:FF:FF:FFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFF,F,FFFFFF,FFFFFFFFFFFFF,FFFFFFFFF:FF:FFFFFFFF:FFF,FFFF:FFFFFFFFFFFFF:FFFFF,,:,:FFFFFFFFFF,FFFFFFFFNM:i:1MD:Z:63G87MC:Z:151MAS:i:146XS:i:65Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:27959:9392145Test_seq17150760151M=171239-419TTTTTTCAGCTATGTTGCAGTTGATTCCCTGGTCCTCCAGAAGTCCCAGTCTACATTCTAATTGGTCGAGTCTGCTCCTCTGACTTTCTATTGCGTTGTCTAATTCTGTAATTTTATTGTTAATCTTTTGGATTTCTACATGCTGTCTCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:3MD:Z:17G46C11T74MC:Z:151MAS:i:136XS:i:47Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1114:13259:9518	Test_seq	305947	Test_seq	306239	+	-	UU	A00126:224:H7HMWDSX3:3:1114:13259:9518161Test_seq30594760151M=306104293GAGTGTTTTTCTAAGTAGTCAAGTGACTGTTCACTTAGATTTAAGACATTTCTCTTCTCCTGTCATCAGCATTAGAGCCCAGATACTAAAGAGGCAAAAGTCATGGAAACCACTGGAGGGCCATTATCTTTCCTCATCTCCCCCATGTTGCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFF:F:FFFFFFFFFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:136M15SAS:i:151XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1114:13259:951881Test_seq30610460136M15S=305947-293TCATCACAAAGGTCTGCGGTTGGTAAAGAGAGGAACTGGATGAAAGGGTTTTGATGGGTTGTTCAAATCTACCACCAAGGCTGTAAACAACTCCCTGAATGAAAGCATCATACTTACTCTTCTTCTAGCCTGGATTTAAAAAAGGGGGGAAFFF:FFFFF,FFFFFFFFFF:FFFFFFFFFFF:FFF,FFFF:FF:FFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFF,FF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:136MC:Z:151MAS:i:136XS:i:0Yt:Z:UU
@@ -574,7 +574,7 @@
 A00126:224:H7HMWDSX3:3:1115:1967:25974	Test_seq	167150	Test_seq	167599	+	-	UU	A00126:224:H7HMWDSX3:3:1115:1967:2597497Test_seq16715018151M=167449450TTTGCATTTCTCTAATGGCTAATGATCGGGAGCATTTTCTCATGTATCTGTTAGCTGCCTGAATATCTTCTTTAGCGAAATGTGTGTTCATGTCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTCACAGAATFFFFFFFFFFFFFF:F,,FFFFF:FFFFFFFFFFFFFFFF:FFFFFFFF,FFFFFFFF:FFF,FFF:FF,FFFFFFFFFFF,FFFFFFFFF,F,,FFFF:::FFF:FFFF,FFFF,F:FFFF:F,,FFFF,FFFFFFFFF:FF:F:FFF:FNM:i:6MD:Z:28A23G22T15A1T49G7MC:Z:151MAS:i:121XS:i:111XA:Z:Test_seq,-213734,151M,8;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:1967:25974145Test_seq16744960151M=167150-450TCTTCATCATTTTTGGTAATGTTTTGTATTCTGTTTATGCCCTGTATTAGGGCTCCTAGGGTTGATCCTATTTTTTCTTCCATGATCTTTATCGTTTTAGTCTTTATGTTTAGGTCTTTGATCCACTTGGAGTTAGTTTTTGTGCATGGTG:FFF:FFF,:FFFFF:FFFFF:FFFFF,FFFFF:FFFFFF:FFFF:FFFF:FFF,FFFFFFFFFFF,FFFFFFFFFFFF:FFFFFFFFFFFF,F,FFFFF:::FFFFFFFF:F:F,FFFFFFF:FF:FFFFFFFFFFFF:FFFFFFFFFF,NM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:101Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1115:7789:25974	!	0	Test_seq	167947	-	-	NR	A00126:224:H7HMWDSX3:3:1115:7789:25974161Test_seq1675683432S119M=167797380CATATTTATGCCTATTACCAAGAAAGGTGATCGATCCACTTGGAGTTAGTTTTTGTGCATGGTGTGAGGTATGGGTCCTGTTTCATTCTTTTGCAAATGGATATCCAGCTATGCCAGCACCATTTGTTAAAAAGACTATCATTTCCCCAATFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FNM:i:1MD:Z:76T42MC:Z:151MAS:i:114XS:i:99XA:Z:Test_seq,-213352,108M2I9M32S,4;Yt:Z:NR	A00126:224:H7HMWDSX3:3:1115:7789:2597481Test_seq16779760151M=167568-380CAGGCTGTTTTGACTACTGTAGCTGTATAATAGGTTCTGAAATCAGGTAGAGTGAGGCCTCCCACTTTCTTCTTCTTTTTCAGTAATGCTTTGCTTATCCGGGGGTTCTTTCCCTTCCATATGAAATTAGTGATTTGTTTCTCTATCCCCTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:21C129MC:Z:32S119MAS:i:146XS:i:113Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1115:18032:26678	Test_seq	170112	Test_seq	170470	+	-	UU	A00126:224:H7HMWDSX3:3:1115:18032:26678161Test_seq17011260151M=170320359TGCATAAATATTTAATATGGTTATGTCTTCCTGATCAATTGTCCCTTTTATCATTATATAGTGTCCTTCTTTATCCTTTGTGGCGGATTTAAGTCTAAAGTCTATTTTGTCAGAAATTAATATTGCTACTCCTCTTCTTTTTTGCTTATTAFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFF,FFFFFFFFFFFFFFFFFFFFF:F,:FFFFFFFFFFFFFFFFFFF:,FFFFFFFFFFFF:FFFFFFFFF:FF:FF:FFF::FFFFFFFFFFF,FFF:FFNM:i:3MD:Z:3G20A59A66MC:Z:151MAS:i:137XS:i:53Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:18032:2667881Test_seq17032060151M=170112-359CTAAGGTGTGTCTCTTGTAGGCAGCATATAGATGGATCGTGTTTCTTTATCCAGTCTGTGACTCTCTGTCTCTTTATTGGTGCATTTAGTCCATTTACATTCAGCGTAATTATAGATAAATAAGTTTTTAGTGCTGTCATTTTGATGCCTTFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:56C94MC:Z:151MAS:i:146XS:i:48Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1115:6686:27383	Test_seq	166310	Test_seq	166674	+	-	RU	A00126:224:H7HMWDSX3:3:1115:6686:2738397Test_seq16631060151M=166550365CCCAGGCGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:F,FF,FNM:i:1MD:Z:6T144MC:Z:26S125MAS:i:146XS:i:27Yt:Z:RU	A00126:224:H7HMWDSX3:3:1115:6686:27383145Test_seq1665506026S125M=166310-365TTATCCCGGAAACCTCTTTGCTTTTAGATCAATAAAAAACCCTCTCCCTCCCTTCCTCCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTACTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCFFFFFFFFFFFFFFF::,FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFF:FFFFFFNM:i:3MD:Z:27C50T9C36MC:Z:151MAS:i:110XS:i:63SA:Z:Test_seq,166445,-,27M124S,48,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1115:6686:273832193Test_seq1664454827M124H=166310-162TTATCCCGGAAACCTCTTTGCTTTTAGFFFFFFFFFFFFFFF::,FFFFFFFFFNM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,166550,-,26S125M,60,3;Yt:Z:RU
+A00126:224:H7HMWDSX3:3:1115:6686:27383	Test_seq	166310	Test_seq	166674	+	-	RU	A00126:224:H7HMWDSX3:3:1115:6686:2738397Test_seq16631060151M=166550365CCCAGGCGCCACAATTCAAAGTGGGATGCAGAATGTTTTCATAATAGAATTATTTTGCCCGTTGACTTAGAAGTCTCCTCAAACCATGTTCCCCAGACCCCAGCCCCTGCTCCGCTGACCTTTGAAGCTTTCATTTTATCCCGGAAACCTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:F,FF,FNM:i:1MD:Z:6T144MC:Z:26S125MAS:i:146XS:i:27Yt:Z:RU	A00126:224:H7HMWDSX3:3:1115:6686:273832193Test_seq1664454827M124H=166310-162TTATCCCGGAAACCTCTTTGCTTTTAGFFFFFFFFFFFFFFF::,FFFFFFFFFNM:i:0MD:Z:27MC:Z:151MAS:i:27XS:i:0SA:Z:Test_seq,166550,-,26S125M,60,3;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1115:6686:27383145Test_seq1665506026S125M=166310-365TTATCCCGGAAACCTCTTTGCTTTTAGATCAATAAAAAACCCTCTCCCTCCCTTCCTCCCTCCCCCCTTTGTAACCACAAAAGTATGTGTTCTTCTCCGTTTTTACTATTTCTCAAGATCTTATAATAGTGGTCTTATACAATATTTGTCCFFFFFFFFFFFFFFF::,FFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFF:FFFFFFNM:i:3MD:Z:27C50T9C36MC:Z:151MAS:i:110XS:i:63SA:Z:Test_seq,166445,-,27M124S,48,0;Yt:Z:RU
 A00126:224:H7HMWDSX3:3:1115:27516:27915	Test_seq	169316	Test_seq	169778	+	-	UU	A00126:224:H7HMWDSX3:3:1115:27516:27915161Test_seq16931660151M=169628463TAGTCAGTCTAGCCAATGGTTTATCAATTTTGTTAATGTTTTCAAAGAACCAGCTTTTGGCTTTGTTAATTCTTTCAATTGTTTTTCTGTTCTCTAATTCATTTAGTTCAGCTCTAATTTTTATTATTTGTTTTCTTCTGGTGCCTGATGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFF:FFFF:FFFFFFFFFFFFFFFFFFFFF:F,FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF,F:FFFFF,FFFF,FFFF::FFFFFF,FFF,FFNM:i:1MD:Z:37T113MC:Z:151MAS:i:146XS:i:107Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:27516:2791581Test_seq16962860151M=169316-463CGTTGCATTCTATGAATTTCCTTATTCCCTCCTTGATGTCTTCTATAACCCAGTCTTTTTTCAGGAGGGTATTGTTCATTTTCCAAGTATTTGATTTCTTTTCCCTAGTTTTTCTGTTATTGATCTCTAGTTTTATTGCCTTGTGGTCTGAFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:8MD:Z:0T5T5A22G83C4T5C2C17MC:Z:151MAS:i:115XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1115:31584:28040	!	0	Test_seq	167925	-	+	NR	A00126:224:H7HMWDSX3:3:1115:31584:2804081Test_seq16821860128M23S=167925-421TCTTTTTGTTGATGTAGAGGAATCCAGGTGATTTTTGTATGTTTATTTTATAACCTGAGACTCTTCCAAACTCTTCTATTACTTTCAGTAGTTCTCTGGAGGATTCCTTAGGGTTTTCCATGTATACGATCAATCATTTCTTCATTTGCTC:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:4MD:Z:26A26T27G11T34MC:Z:151MAS:i:108XS:i:31Yt:Z:NR	A00126:224:H7HMWDSX3:3:1115:31584:28040161Test_seq16792560151M=168218421AGTGATTTGTTTCTCTATCCCCTTAAAATATGACATTGGTATTTGGATTGGAAGTGCGTTATATGTATAGATGGCTTTTGGTAGAATAGACATTTTTACTATGTTAAGTCTTCCTATCCATGAGCAGGGTATGTTTTTCCACTTAAGTATGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FF,FFFFFFFFFFFFFFFFF:FFNM:i:1MD:Z:135C15MC:Z:128M23SAS:i:146XS:i:73Yt:Z:NR
 A00126:224:H7HMWDSX3:3:1115:15944:29731	Test_seq	166867	Test_seq	167319	+	-	UU	A00126:224:H7HMWDSX3:3:1115:15944:2973197Test_seq16686738151M=167169453GCTGCAATAAACATGGGTGTGCATATATCTGTTTGTATGAAGGCTCTTGTATCTCTAGGGTCTATTCCTAGGAGTGGGATTTCTGGGTTGTATGGTAGTTCTATTTCTAACTGTTTAAGATAACGCCAGATGGATTTCCAAAGTGGTTGTAFFFFF,,FFFFFF:FFF:FFFFF:FFFFFFFF,FFF:FFF:FFF:F::FFFFFFF::FFF,,::FFFFFFFFFFFFFF:,::F:FFF,FFF,,FF,,FFFFF,FFFFFFFFFFF,FFFFFFF,F:FF:FFFFFFF:FFF::FFFFFFFFF,NM:i:5MD:Z:26G9G13T10A8T80MC:Z:151MAS:i:126XS:i:106XA:Z:Test_seq,-214017,151M,9;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1115:15944:29731145Test_seq16716952151M=166867-453TAATGATCGAGAGCATTTTCTCATGTATCTGTTGGCTGCCTGAATATCTTCTTTAGTGAAATGTGTGTTCATATCCTTTGCCCACTTCTTGATTGGGTTGTTTGTCTTTTTGTGGTTGAGTTTTGACAGAATCATGTAGATTTTAGAGATC:FF,FF,F:FFF:FFFF,FFFFF,FF:FF:FFFFFFFFFF::FF:FFF:FFF:F,FFFFFF,F:FFFFFFF:FFFFFFFFFFFFF,FFFFFF:FFFF:FFF,FFFFFFFFFFFFFFFF:FFFFF:FFFFFFFF:,FFFFFFFF:FFFFFFFNM:i:1MD:Z:74T76MC:Z:151MAS:i:146XS:i:121XA:Z:Test_seq,+213715,151M,6;Yt:Z:UU
@@ -590,7 +590,7 @@
 A00126:224:H7HMWDSX3:3:1116:21097:1329	Test_seq	167966	Test_seq	212718	+	+	UU	A00126:224:H7HMWDSX3:3:1116:21097:132965Test_seq16796621151M=21271844753TTTGGATCGGAATTGCATTGTATGTATAGATGGCTTTTGGTAGAATAGACATTTTGACTATGTTAAGTCTTCCTATCCATGAGCAAGGTATGTTTTTCCACTTAAGTATGTCCTTTTGAATTTCTTGTACTAGAGCTTTGTAGTTTTCTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFNM:i:11MD:Z:7T4G3G2A35T29G8C34G0C4T3A11MC:Z:151MAS:i:96XS:i:83XA:Z:Test_seq,-212922,151M,14;Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:21097:1329129Test_seq21271831151M=167966-44753TCCTCCAGAAAACTACTGAAACTAATAGAAGAGTTTGGCAGAGTCTCAGGTTATAAGATAAACATACAAAAATCACTTGGATTCCTCTACATCAACAAAAAGAACATCGAAGAGGAAATAACCAAATCAATACCATTCACAGTAGCCCCCAFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:108A42MC:Z:151MAS:i:146XS:i:131XA:Z:Test_seq,-168170,151M,4;Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:9525:1548	Test_seq	165792	Test_seq	166199	+	-	UU	A00126:224:H7HMWDSX3:3:1116:9525:1548161Test_seq16579260110M2D41M=166049408CCGTCTTCCCTCTCTCTCCATCTTCCCATCCCTCCTCCAGTCAAGAGTTGCCAACACACTCTCCCGTGTCCACCTGATTTAATTAGCTCACTCTTCCTCAGCATCTCTCTCCCCCCCACTGACCAGTCCTTTTCATGCCTGATGAGTTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:12MD:Z:2A5T9T13C31A10A8G11A13^CC7G19T13MC:Z:151MAS:i:95XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:9525:154881Test_seq16604960151M=165792-408ATTTGGGGTCTGTATCCCATTGGTCTCCTGCTCCCTCAGGAGTTGTCTGTTGTACTCCCTGACAGGGCAGACATCGATTGTGGCCGGGCACCAACTAGTTCTTCTGGTCTCAGGATAATGTAGGTCTCTGGTTCATGTGGCCCTTTCTGTCFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:1MD:Z:53G97MC:Z:110M2D41MAS:i:146XS:i:50Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:28917:2190	Test_seq	74270	Test_seq	74579	+	-	UU	A00126:224:H7HMWDSX3:3:1116:28917:219097Test_seq7427060151M=74429310TGGGAGTCCAGGGAAGCCTTCTGGAGACCAAATCTGTTACCATCCGACTCATAGTGACCCTATAGGACAGAATAGAACTCCCCCATAGGGTTTCCAAGGCTGTAAACCTTTACAGAAGCAGATTGCCATGTCTTTCTCCCACGAAGCAGCTFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:41Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:28917:2190145Test_seq7442960151M=74270-310CGAACCGCGGACCTTTCAGTCAGCAGCCGAGTGCTTCACCACCGTACCACCAGGGCTCCCCTCCTGCGGAAAGTGATGCATAAACTGAAATCTGACCAATACATAGACTCAGCCCCCAGGATAAGAGGAACAAGAGCAGGAGTGTTCTAGGFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF:F:,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFNM:i:0MD:Z:151MC:Z:151MAS:i:151XS:i:0Yt:Z:UU
-A00126:224:H7HMWDSX3:3:1116:3495:3724	Test_seq	335700	Test_seq	436771	-	-	UR	A00126:224:H7HMWDSX3:3:1116:3495:3724177Test_seq33566960119S32M=436621101072AAACTGGTGTTCAAATGTGTTTTACCTTTTGGAAGAAAAATATGATTTTCTAAAGGAGGAAAATAAGAAAAAGTATAGATTTTTCATAGTTTCACTTTTCACAATGTGGAAAAAAGATCGATCTATCCTGAAGTACCTTAAGGTTAGTGGAFF:FF:FFFFF,FFFFFFFFFFFFF:FFFFF,FFFF,FF:FFFF,FFF:FFF:FF:F,FFF:FFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,436401,+,32S119M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1116:3495:37242209Test_seq4364016032H119M=436621371GATCTTTTTTCCACATTGTGAAAAGTGAAACTATGAAAAATCTATACTTTTTCTTATTTTCCTCCTTTAGAAAATCATATTTTTCTTCCAAAAGGTAAAACACATTTGAACACCAGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:FFF,F:FF:FFF:FFF,FFFF:FF,FFFF,FFFFF:FFFFFFFFFFFFF,FFFFF:FF:FFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,335669,-,119S32M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1116:3495:3724113Test_seq43662160151M=335669-101072AAAAATGCCACGTTGGATTAACCTTGACTCAACTGGACATGTAGATACCCCATGTGGATGGTTAGAGGGGTGGCAAGGTCCATCCTCCCCAAAGAGACTGTATCCCTACCTGCCCGAGGACCTGAAGTGGTTACGTGTCCAACCAGTTGCCFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:151MC:Z:119S32MAS:i:151XS:i:0Yt:Z:UR
+A00126:224:H7HMWDSX3:3:1116:3495:3724	Test_seq	335700	Test_seq	436771	-	-	UR	A00126:224:H7HMWDSX3:3:1116:3495:37242209Test_seq4364016032H119M=436621371GATCTTTTTTCCACATTGTGAAAAGTGAAACTATGAAAAATCTATACTTTTTCTTATTTTCCTCCTTTAGAAAATCATATTTTTCTTCCAAAAGGTAAAACACATTTGAACACCAGTTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFF:FFFFFFF:FFF,F:FF:FFF:FFF,FFFF:FF,FFFF,FFFFF:FFFFFFFFFFFFF,FFFFF:FF:FFNM:i:0MD:Z:119MC:Z:151MAS:i:119XS:i:0SA:Z:Test_seq,335669,-,119S32M,60,0;Yt:Z:URNEXT_SAMA00126:224:H7HMWDSX3:3:1116:3495:3724177Test_seq33566960119S32M=436621101072AAACTGGTGTTCAAATGTGTTTTACCTTTTGGAAGAAAAATATGATTTTCTAAAGGAGGAAAATAAGAAAAAGTATAGATTTTTCATAGTTTCACTTTTCACAATGTGGAAAAAAGATCGATCTATCCTGAAGTACCTTAAGGTTAGTGGAFF:FF:FFFFF,FFFFFFFFFFFFF:FFFFF,FFFF,FF:FFFF,FFF:FFF:FF:F,FFF:FFFFFFF:FFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:32MC:Z:151MAS:i:32XS:i:0SA:Z:Test_seq,436401,+,32S119M,60,0;Yt:Z:UR	A00126:224:H7HMWDSX3:3:1116:3495:3724113Test_seq43662160151M=335669-101072AAAAATGCCACGTTGGATTAACCTTGACTCAACTGGACATGTAGATACCCCATGTGGATGGTTAGAGGGGTGGCAAGGTCCATCCTCCCCAAAGAGACTGTATCCCTACCTGCCCGAGGACCTGAAGTGGTTACGTGTCCAACCAGTTGCCFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:FFFFFFF:FFFFFF:FFFFFFFFFFFF:FFFF:FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF:FFFFFFFFFFFFFFF::FFFFFFFFFFF:FFFFFFFFFNM:i:0MD:Z:151MC:Z:119S32MAS:i:151XS:i:0Yt:Z:UR
 A00126:224:H7HMWDSX3:3:1116:1886:5885	Test_seq	133235	Test_seq	133657	+	-	UU	A00126:224:H7HMWDSX3:3:1116:1886:588597Test_seq13323560151M=133507423AATCATGGTCCAGCCAAGTTGGCACATAAGGTTAACAATCATAGCCAGCGTTACTCTTGCCTTGCACCTCGGTGTTCGTTACTGCCAGATGTACGTCGTTAATGTGCGTAACAGTCAGATACGAGATTTTTTACTATTGTCATGAACGTAAFFFFFFF:FFFF,FFFFFFF,FFFFF:FF,FFFF::,FFFFFFFFFF,F:FFFFFFFFF,FFFFFF,FFF,FFFFFF,FFFFF,FFFFFFFFFF:FFFFFFFFFF:::FFF,FFF:F,FFFF,F:FFFFFF:F,FFFF,F,FF::F,FFFFNM:i:1MD:Z:36C114MC:Z:151MAS:i:146XS:i:0Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:1886:5885145Test_seq13350760151M=133235-423GGGGTTTCACTGTCTGGTTTTTAGAAGCAGCATGCCAGGTCTCTGTTCCCTAGTCCACCTTAGTCTGGACACTCCGCTGAAACCTGTCCAGTGTCATAGCGACACACAGGCCTACACTGAGAGACAAGTGGTGGATGAATTTGAAGTACGT:F::FFFFFFFF,FF,FFF,,F:FFF,FFFFFF,FFF,:F:F::FFFF,FFF:F,:FFFFFFFF:,,F,:FF:FFFFFFFFFFFFFF:FFFF:F:FFFFF::FFFFFFFFF,F,FFFFFFFFFF,FFFFFFFFF,FF,F:FFFFFFFFF,FNM:i:5MD:Z:12G29T70C20C2C13MC:Z:151MAS:i:126XS:i:0Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:5041:6715	Test_seq	168462	Test_seq	168810	+	-	UU	A00126:224:H7HMWDSX3:3:1116:5041:671597Test_seq16846260151M=168674349GAGCGGTGATAAAGGGCATCCTTGTCTGGTTCCCGTTCTCAAGGGAAATGCTTTCAGGTTCTCTCCATTTAGAGTGATATTGGCTGTTGGCTTTGCATAGATGCCCTTTATTATGTTGAGGAATTTTCCTTCAATTCCTATTTTGGTAAGAFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFF::F:F:F,FFFFFFFFFFFF:F:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFF,FFF:FFFFFF:FF:FFFF,FFFFFFFF:FFF,F:,FFFFF:FFFNM:i:1MD:Z:33T117MC:Z:137M14SAS:i:146XS:i:91Yt:Z:UU	A00126:224:H7HMWDSX3:3:1116:5041:6715145Test_seq16867460137M14S=168462-349TCATGTGGTTTTTATCTTTTGTTTTATTTATGTGATGGATTACATTAATGGTTCTTTTGATATTGAACCAGCCTTGCATACCTGATATAAATCCCACTTGATCAGGGTGAATTATTTTTTTGATGTGTTGTTGGATTGATCTCCGGGACTTF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FF:F:FFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:5MD:Z:42T10T2C7A19G52MC:Z:151MAS:i:112XS:i:30Yt:Z:UU
 A00126:224:H7HMWDSX3:3:1116:31720:7545	Test_seq	303522	Test_seq	305328	+	+	RU	A00126:224:H7HMWDSX3:3:1116:31720:7545129Test_seq30352260151M=3053281807ATCTTTTATTTGGGAGAAACAAGTCAGAATTAATTCCTGTTGATTGCACTGAATTATAAATGATGAAGAATCAGAAATCAGCTACTGGCCCACAGGCATGTTGCAGAAACTCAATAAATGTTAGCCATCATCTCCTTTTTCATCATCATAAFFFFFFF:FFFF:F:FFFFFFFFFF:FFF:FFFF:FFFF,FFFFFFFFF,FFFFF::FFF:FFFFFFFFFFFFFFF,F:FFFFFFFFFF:FF:FF:FFFF,FFF:FFF:FFFFFF:FFFFFF,F:F,FFFFF:FFF:FFF:FFFF,FFF:FNM:i:0MD:Z:151MC:Z:20M131SAS:i:151XS:i:0Yt:Z:RU	A00126:224:H7HMWDSX3:3:1116:31720:754565Test_seq305328620M131S=303522-1807CCATTGCTATCAAGTGGATTGATCACTCCCTGGAGAAGGACATCATGCTTGGTAAAGTAGAGGGCCAACATGGATTGACACAGTGGCTGCAACAATGGGCTCAAGCATAACAACAACTGTGAGGATGGCGCAGGGCTGGGCAGTGTTTCATFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFF:FFFFFFFFFFF:FF:FFF:FFFFF:F:FFFFFFFFF,F::FFFFFF:FFFFFFFFFFFFFFF,F:FFFFF:FFFFFFF:FFFFFFFFFFFFFFFFFNM:i:0MD:Z:20MC:Z:151MAS:i:20XS:i:0SA:Z:Test_seq,303836,-,131M20S,60,0;Yt:Z:RUNEXT_SAMA00126:224:H7HMWDSX3:3:1116:31720:75452129Test_seq30383660131M20H=303522-445ATGAAACACTGCCCAGCCCTGCGCCATCCTCACAGTTGTTGTTATGCTTGAGCCCATTGTTGCAGCCACTGTGTCAATCCATGTTGGCCCTCTACTTTACCAAGCATGATGTCCTTCTCCAGGGAGTGATCFFFFFFFFFFFFFFFFF:FFFFFFF:FFFFF:F,FFFFFFFFFFFFFFF:FFFFFF::F,FFFFFFFFF:F:FFFFF:FFF:FF:FFFFFFFFFFF:FFFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFNM:i:0MD:Z:131MC:Z:151MAS:i:131XS:i:43SA:Z:Test_seq,305328,+,20M131S,6,0;Yt:Z:RU
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/output_sorted_pairs.pairs
--- a/test-data/output_sorted_pairs.pairs	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/output_sorted_pairs.pairs	Tue Apr 30 12:02:01 2024 +0000
@@ -38,18 +38,18 @@
 #samheader: @SQ	SN:chrXV	LN:1091291
 #samheader: @SQ	SN:chrXVI	LN:948066
 #samheader: @PG	ID:bwa	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.00.1.fastq.gz MATalpha_R1.lane1.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/c/9/dataset_5c90f563-7928-4455-84af-6129feb92ffc.dat -c /tmp/tmpvtvmbj0m/files/4/9/a/dataset_49af402e-cee9-4737-bf5e-15a04f62c1d8.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/7/outputs/dataset_461ac430-f019-4ae5-9b37-796f7c45fa35.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.0.3
-#samheader: @PG	ID:pairtools_sort-1.3	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmpwmd1r52h/files/d/5/c/dataset_d5c01101-89f1-4667-9536-e0a8f57684e3.dat -o /tmp/tmpwmd1r52h/job_working_directory/000/2/outputs/dataset_b446f275-b840-40de-9fb8-08bd13d19337.dat --nproc-in 1 --nproc-out 1	PP:pairtools_parse-1.2	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-1.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/e/9/dataset_be9fce34-5096-400c-9efd-1f189da7f40a.dat -c /tmp/tmpchj83off/files/1/4/3/dataset_143017f3-646f-4cc1-b3d6-fc5d73287981.dat -o /tmp/tmpchj83off/job_working_directory/000/7/outputs/dataset_3348ea1d-0460-452f-aafb-dbde21a7b812.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa	VN:1.1.0
+#samheader: @PG	ID:pairtools_sort-1.3	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmptyq9evah/files/8/6/3/dataset_863ae21a-b207-4ba9-b94f-54406023b20a.dat -o /tmp/tmptyq9evah/job_working_directory/000/2/outputs/dataset_de1b3c74-45c1-408a-bb81-f91bacfb79cd.dat --nproc-in 1 --nproc-out 1	PP:pairtools_parse-1.2	VN:1.1.0
 #samheader: @PG	ID:bwa-2CCE5976	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane1.01.1.fastq.gz MATalpha_R1.lane1.01.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/c/9/dataset_5c90f563-7928-4455-84af-6129feb92ffc.dat -c /tmp/tmpvtvmbj0m/files/4/9/a/dataset_49af402e-cee9-4737-bf5e-15a04f62c1d8.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/7/outputs/dataset_461ac430-f019-4ae5-9b37-796f7c45fa35.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.0.3
-#samheader: @PG	ID:pairtools_sort-2.3	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmpwmd1r52h/files/d/5/c/dataset_d5c01101-89f1-4667-9536-e0a8f57684e3.dat -o /tmp/tmpwmd1r52h/job_working_directory/000/2/outputs/dataset_b446f275-b840-40de-9fb8-08bd13d19337.dat --nproc-in 1 --nproc-out 1	PP:pairtools_parse-2.2	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-2.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/e/9/dataset_be9fce34-5096-400c-9efd-1f189da7f40a.dat -c /tmp/tmpchj83off/files/1/4/3/dataset_143017f3-646f-4cc1-b3d6-fc5d73287981.dat -o /tmp/tmpchj83off/job_working_directory/000/7/outputs/dataset_3348ea1d-0460-452f-aafb-dbde21a7b812.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-2CCE5976	VN:1.1.0
+#samheader: @PG	ID:pairtools_sort-2.3	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmptyq9evah/files/8/6/3/dataset_863ae21a-b207-4ba9-b94f-54406023b20a.dat -o /tmp/tmptyq9evah/job_working_directory/000/2/outputs/dataset_de1b3c74-45c1-408a-bb81-f91bacfb79cd.dat --nproc-in 1 --nproc-out 1	PP:pairtools_parse-2.2	VN:1.1.0
 #samheader: @PG	ID:bwa-3CAFD9D9	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.00.1.fastq.gz MATalpha_R1.lane2.00.2.fastq.gz
-#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/c/9/dataset_5c90f563-7928-4455-84af-6129feb92ffc.dat -c /tmp/tmpvtvmbj0m/files/4/9/a/dataset_49af402e-cee9-4737-bf5e-15a04f62c1d8.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/7/outputs/dataset_461ac430-f019-4ae5-9b37-796f7c45fa35.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.0.3
-#samheader: @PG	ID:pairtools_sort-3.3	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmpwmd1r52h/files/d/5/c/dataset_d5c01101-89f1-4667-9536-e0a8f57684e3.dat -o /tmp/tmpwmd1r52h/job_working_directory/000/2/outputs/dataset_b446f275-b840-40de-9fb8-08bd13d19337.dat --nproc-in 1 --nproc-out 1	PP:pairtools_parse-3.2	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-3.2	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/e/9/dataset_be9fce34-5096-400c-9efd-1f189da7f40a.dat -c /tmp/tmpchj83off/files/1/4/3/dataset_143017f3-646f-4cc1-b3d6-fc5d73287981.dat -o /tmp/tmpchj83off/job_working_directory/000/7/outputs/dataset_3348ea1d-0460-452f-aafb-dbde21a7b812.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:bwa-3CAFD9D9	VN:1.1.0
+#samheader: @PG	ID:pairtools_sort-3.3	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmptyq9evah/files/8/6/3/dataset_863ae21a-b207-4ba9-b94f-54406023b20a.dat -o /tmp/tmptyq9evah/job_working_directory/000/2/outputs/dataset_de1b3c74-45c1-408a-bb81-f91bacfb79cd.dat --nproc-in 1 --nproc-out 1	PP:pairtools_parse-3.2	VN:1.1.0
 #samheader: @PG	ID:bwa-4548A671	PN:bwa	VN:0.7.15-r1140	CL:bwa mem -t 8 -v 3 -SP sacCer3.fa.gz MATalpha_R1.lane2.01.1.fastq.gz MATalpha_R1.lane2.01.2.fastq.gz
 #samheader: @PG	ID:samtools	PN:samtools	PP:bwa-4548A671	VN:1.19.2	CL:samtools view -s 0.1 -b -@ 4 -o subset.bam test.bam
-#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpvtvmbj0m/files/5/c/9/dataset_5c90f563-7928-4455-84af-6129feb92ffc.dat -c /tmp/tmpvtvmbj0m/files/4/9/a/dataset_49af402e-cee9-4737-bf5e-15a04f62c1d8.dat -o /tmp/tmpvtvmbj0m/job_working_directory/000/7/outputs/dataset_461ac430-f019-4ae5-9b37-796f7c45fa35.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.0.3
-#samheader: @PG	ID:pairtools_sort-4.4	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmpwmd1r52h/files/d/5/c/dataset_d5c01101-89f1-4667-9536-e0a8f57684e3.dat -o /tmp/tmpwmd1r52h/job_working_directory/000/2/outputs/dataset_b446f275-b840-40de-9fb8-08bd13d19337.dat --nproc-in 1 --nproc-out 1	PP:pairtools_parse-4.3	VN:1.0.3
+#samheader: @PG	ID:pairtools_parse-4.3	PN:pairtools_parse	CL:/usr/local/bin/pairtools parse /tmp/tmpchj83off/files/b/e/9/dataset_be9fce34-5096-400c-9efd-1f189da7f40a.dat -c /tmp/tmpchj83off/files/1/4/3/dataset_143017f3-646f-4cc1-b3d6-fc5d73287981.dat -o /tmp/tmpchj83off/job_working_directory/000/7/outputs/dataset_3348ea1d-0460-452f-aafb-dbde21a7b812.dat --min-mapq 1 --max-molecule-size 750 --walks-policy mask --max-inter-align-gap 20 --nproc-in 1 --nproc-out 1	PP:samtools	VN:1.1.0
+#samheader: @PG	ID:pairtools_sort-4.4	PN:pairtools_sort	CL:/usr/local/bin/pairtools sort /tmp/tmptyq9evah/files/8/6/3/dataset_863ae21a-b207-4ba9-b94f-54406023b20a.dat -o /tmp/tmptyq9evah/job_working_directory/000/2/outputs/dataset_de1b3c74-45c1-408a-bb81-f91bacfb79cd.dat --nproc-in 1 --nproc-out 1	PP:pairtools_parse-4.3	VN:1.1.0
 #columns: readID chrom1 pos1 chrom2 pos2 strand1 strand2 pair_type sam1 sam2
 HWUSI-EAS1533_0033_FC:1:1:1193:16038	!	0	!	0	-	-	MM	HWUSI-EAS1533_0033_FC:1:1:1193:1603881chrIV1529635036MchrIX187220AATTGACATTAAGCAATTGATGATGGTGATCATGCT@@@@@@C@@C@C@@CCCCC@@CC@CC7777832324NM:i:0MD:Z:36AS:i:36XS:i:36Yt:Z:MM	HWUSI-EAS1533_0033_FC:1:1:1193:16038161chrIX18722036MchrIV15296350NAAGTAAATTTACGATCTGGAAGGAGTGCTGTATGT&,,,,22032@@@@@@@@@@@@@@@@@@@@@@@@@@NM:i:2MD:Z:0A11G23AS:i:30XS:i:30XA:Z:chrX,+18705,36M,2;chrXV,+24474,36M,2;Yt:Z:MM
 HWUSI-EAS1533_0033_FC:1:1:2292:14294	!	0	!	0	-	-	MM	HWUSI-EAS1533_0033_FC:1:1:2292:1429481chrVII1001809036M=1001598-247ATCGAAGATCCATTTGCTGAAGATGACTGGGAAGCT7@@@@@C@@@C@C@CC@@@@C@C@C@778771111-NM:i:0MD:Z:36AS:i:36XS:i:36XA:Z:chrVIII,-452209,36M,0;Yt:Z:MM	HWUSI-EAS1533_0033_FC:1:1:2292:14294161chrVII1001598036M=1001809247NGCTTTGGACTTGATTGTTGACGCTATCAAGGCTGC)333377777@C@@@C@CCC@@@@C@C@@CC@@@@@NM:i:1MD:Z:0A35AS:i:35XS:i:35XA:Z:chrVIII,+451998,36M,1;Yt:Z:MM
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/pairs_output.stats
--- a/test-data/pairs_output.stats	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/pairs_output.stats	Tue Apr 30 12:02:01 2024 +0000
@@ -29,6 +29,33 @@
 summary/frac_cis_40kb+	0.1383219954648526
 summary/frac_dups	0.0
 summary/complexity_naive	nan
+summary/dist_freq_convergence/convergence_dist	421697
+summary/dist_freq_convergence/strands_w_max_convergence_dist	++
+summary/dist_freq_convergence/convergence_rel_diff_threshold	0.05
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/++	25
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/--	26
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/-+	7
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/+-	383
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist_all_strands	441
+summary/dist_freq_convergence/n_cis_pairs_above_convergence_dist_all_strands	0
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/--	0.05895691609977324
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/-+	0.015873015873015872
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/+-	0.8684807256235828
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_cis_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/--	0.05895691609977324
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/-+	0.015873015873015872
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/+-	0.8684807256235828
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/--	0.05895691609977324
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/-+	0.015873015873015872
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/+-	0.8684807256235828
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_above_convergence_dist_all_strands	0.0
 chrom_freq/Test_seq/Test_seq	441
 dist_freq/0-1/+-	0
 dist_freq/0-1/-+	0
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+dist_freq/1000000000+/++	0
 chromsizes/Test_seq	450060
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/pairs_output_merged.stats
--- a/test-data/pairs_output_merged.stats	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/pairs_output_merged.stats	Tue Apr 30 12:02:01 2024 +0000
@@ -7,28 +7,55 @@
 cis	882
 trans	0
 pair_types/NU	2
-pair_types/UR	38
+pair_types/RU	34
 pair_types/MR	12
-pair_types/MU	42
 pair_types/UU	810
-pair_types/RU	34
 pair_types/NR	238
+pair_types/MU	42
 pair_types/MM	8
+pair_types/UR	38
 cis_1kb+	150
 cis_2kb+	144
 cis_4kb+	140
 cis_10kb+	134
 cis_20kb+	122
 cis_40kb+	122
+summary/frac_cis	1.0
 summary/frac_cis_1kb+	0.17006802721088435
-summary/frac_dups	0.0
-summary/frac_cis_10kb+	0.15192743764172337
-summary/complexity_naive	nan
-summary/frac_cis	1.0
 summary/frac_cis_2kb+	0.16326530612244897
 summary/frac_cis_4kb+	0.15873015873015872
+summary/frac_cis_10kb+	0.15192743764172337
 summary/frac_cis_20kb+	0.1383219954648526
 summary/frac_cis_40kb+	0.1383219954648526
+summary/frac_dups	0.0
+summary/complexity_naive	nan
+summary/dist_freq_convergence/convergence_dist	421697
+summary/dist_freq_convergence/strands_w_max_convergence_dist	++
+summary/dist_freq_convergence/convergence_rel_diff_threshold	0.05
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/++	50
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/--	52
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/-+	14
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/+-	766
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist_all_strands	882
+summary/dist_freq_convergence/n_cis_pairs_above_convergence_dist_all_strands	0
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/--	0.05895691609977324
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/-+	0.015873015873015872
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/+-	0.8684807256235828
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_cis_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/--	0.05895691609977324
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/-+	0.015873015873015872
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/+-	0.8684807256235828
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/--	0.05895691609977324
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/-+	0.015873015873015872
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/+-	0.8684807256235828
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_above_convergence_dist_all_strands	0.0
 chrom_freq/Test_seq/Test_seq	882
 dist_freq/0-1/+-	0
 dist_freq/0-1/-+	0
@@ -42,144 +69,280 @@
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 dist_freq/2-3/--	0
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 chromsizes/Test_seq	450060
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/pairs_output_with_chromsize.stats
--- a/test-data/pairs_output_with_chromsize.stats	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/pairs_output_with_chromsize.stats	Tue Apr 30 12:02:01 2024 +0000
@@ -29,6 +29,33 @@
 summary/frac_cis_40kb+	0.1383219954648526
 summary/frac_dups	0.0
 summary/complexity_naive	nan
+summary/dist_freq_convergence/convergence_dist	421697
+summary/dist_freq_convergence/strands_w_max_convergence_dist	++
+summary/dist_freq_convergence/convergence_rel_diff_threshold	0.05
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/++	25
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/--	26
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/-+	7
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist/+-	383
+summary/dist_freq_convergence/n_cis_pairs_below_convergence_dist_all_strands	441
+summary/dist_freq_convergence/n_cis_pairs_above_convergence_dist_all_strands	0
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/--	0.05895691609977324
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/-+	0.015873015873015872
+summary/dist_freq_convergence/frac_cis_in_cis_below_convergence_dist/+-	0.8684807256235828
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+summary/dist_freq_convergence/frac_cis_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/--	0.05895691609977324
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+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist/+-	0.8684807256235828
+summary/dist_freq_convergence/frac_total_mapped_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_total_mapped_in_cis_above_convergence_dist_all_strands	0.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/++	0.05668934240362812
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/--	0.05895691609977324
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/-+	0.015873015873015872
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist/+-	0.8684807256235828
+summary/dist_freq_convergence/frac_total_nodups_in_cis_below_convergence_dist_all_strands	1.0
+summary/dist_freq_convergence/frac_total_nodups_in_cis_above_convergence_dist_all_strands	0.0
 chrom_freq/Test_seq/Test_seq	441
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 dist_freq/0-1/-+	0
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+dist_freq/10000000-13335214/-+	0
+dist_freq/10000000-13335214/--	0
+dist_freq/10000000-13335214/++	0
+dist_freq/13335214-17782794/+-	0
+dist_freq/13335214-17782794/-+	0
+dist_freq/13335214-17782794/--	0
+dist_freq/13335214-17782794/++	0
+dist_freq/17782794-23713737/+-	0
+dist_freq/17782794-23713737/-+	0
+dist_freq/17782794-23713737/--	0
+dist_freq/17782794-23713737/++	0
+dist_freq/23713737-31622777/+-	0
+dist_freq/23713737-31622777/-+	0
+dist_freq/23713737-31622777/--	0
+dist_freq/23713737-31622777/++	0
+dist_freq/31622777-42169650/+-	0
+dist_freq/31622777-42169650/-+	0
+dist_freq/31622777-42169650/--	0
+dist_freq/31622777-42169650/++	0
+dist_freq/42169650-56234133/+-	0
+dist_freq/42169650-56234133/-+	0
+dist_freq/42169650-56234133/--	0
+dist_freq/42169650-56234133/++	0
+dist_freq/56234133-74989421/+-	0
+dist_freq/56234133-74989421/-+	0
+dist_freq/56234133-74989421/--	0
+dist_freq/56234133-74989421/++	0
+dist_freq/74989421-100000000/+-	0
+dist_freq/74989421-100000000/-+	0
+dist_freq/74989421-100000000/--	0
+dist_freq/74989421-100000000/++	0
+dist_freq/100000000-133352143/+-	0
+dist_freq/100000000-133352143/-+	0
+dist_freq/100000000-133352143/--	0
+dist_freq/100000000-133352143/++	0
+dist_freq/133352143-177827941/+-	0
+dist_freq/133352143-177827941/-+	0
+dist_freq/133352143-177827941/--	0
+dist_freq/133352143-177827941/++	0
+dist_freq/177827941-237137371/+-	0
+dist_freq/177827941-237137371/-+	0
+dist_freq/177827941-237137371/--	0
+dist_freq/177827941-237137371/++	0
+dist_freq/237137371-316227766/+-	0
+dist_freq/237137371-316227766/-+	0
+dist_freq/237137371-316227766/--	0
+dist_freq/237137371-316227766/++	0
+dist_freq/316227766-421696503/+-	0
+dist_freq/316227766-421696503/-+	0
+dist_freq/316227766-421696503/--	0
+dist_freq/316227766-421696503/++	0
+dist_freq/421696503-562341325/+-	0
+dist_freq/421696503-562341325/-+	0
+dist_freq/421696503-562341325/--	0
+dist_freq/421696503-562341325/++	0
+dist_freq/562341325-749894209/+-	0
+dist_freq/562341325-749894209/-+	0
+dist_freq/562341325-749894209/--	0
+dist_freq/562341325-749894209/++	0
+dist_freq/749894209-1000000000/+-	0
+dist_freq/749894209-1000000000/-+	0
+dist_freq/749894209-1000000000/--	0
+dist_freq/749894209-1000000000/++	0
+dist_freq/1000000000+/+-	0
+dist_freq/1000000000+/-+	0
+dist_freq/1000000000+/--	0
+dist_freq/1000000000+/++	0
 chromsizes/Test_seq	450060
diff -r 81fc1418fbfb -r 6cbca5661205 test-data/pairs_output_yaml.stats
--- a/test-data/pairs_output_yaml.stats	Tue Apr 16 12:50:25 2024 +0000
+++ b/test-data/pairs_output_yaml.stats	Tue Apr 30 12:02:01 2024 +0000
@@ -3,10 +3,8 @@
   total_unmapped: 4
   total_single_sided_mapped: 147
   total_mapped: 441
-  total_dups: 0
   total_nodups: 441
   cis: 441
-  trans: 0
   pair_types:
     UU: 405
     NR: 119
@@ -32,6 +30,38 @@
     frac_cis_40kb+: 0.1383219954648526
     frac_dups: 0.0
     complexity_naive: .nan
+    dist_freq_convergence:
+      convergence_dist: 421697
+      strands_w_max_convergence_dist: ++
+      convergence_rel_diff_threshold: 0.05
+      n_cis_pairs_below_convergence_dist:
+        ++: 25
+        --: 26
+        -+: 7
+        +-: 383
+      n_cis_pairs_below_convergence_dist_all_strands: 441
+      n_cis_pairs_above_convergence_dist_all_strands: 0
+      frac_cis_in_cis_below_convergence_dist:
+        ++: 0.05668934240362812
+        --: 0.05895691609977324
+        -+: 0.015873015873015872
+        +-: 0.8684807256235828
+      frac_cis_in_cis_below_convergence_dist_all_strands: 1.0
+      frac_cis_in_cis_above_convergence_dist_all_strands: 0.0
+      frac_total_mapped_in_cis_below_convergence_dist:
+        ++: 0.05668934240362812
+        --: 0.05895691609977324
+        -+: 0.015873015873015872
+        +-: 0.8684807256235828
+      frac_total_mapped_in_cis_below_convergence_dist_all_strands: 1.0
+      frac_total_mapped_in_cis_above_convergence_dist_all_strands: 0.0
+      frac_total_nodups_in_cis_below_convergence_dist:
+        ++: 0.05668934240362812
+        --: 0.05895691609977324
+        -+: 0.015873015873015872
+        +-: 0.8684807256235828
+      frac_total_nodups_in_cis_below_convergence_dist_all_strands: 1.0
+      frac_total_nodups_in_cis_above_convergence_dist_all_strands: 0.0
   chrom_freq:
     Test_seq/Test_seq: 441
   dist_freq:
@@ -40,156 +70,292 @@
       1: 0
       2: 0
       3: 0
+      4: 0
       6: 0
+      7: 0
       10: 0
+      13: 0
       18: 0
-      32: 7
-      56: 9
-      100: 5
-      178: 60
-      316: 284
-      562: 1
-      1000: 1
-      1778: 1
+      24: 0
+      32: 0
+      42: 7
+      56: 7
+      75: 2
+      100: 3
+      133: 2
+      178: 3
+      237: 57
+      316: 171
+      422: 113
+      562: 0
+      750: 1
+      1000: 0
+      1334: 1
+      1778: 0
+      2371: 1
       3162: 0
-      5623: 2
-      10000: 2
+      4217: 0
+      5623: 0
+      7499: 2
+      10000: 1
+      13335: 1
       17783: 1
+      23714: 0
       31623: 0
-      56234: 3
-      100000: 3
-      177828: 4
+      42170: 0
+      56234: 2
+      74989: 1
+      100000: 1
+      133352: 2
+      177828: 3
+      237137: 1
       316228: 0
+      421697: 0
       562341: 0
+      749894: 0
       1000000: 0
+      1333521: 0
       1778279: 0
+      2371374: 0
       3162278: 0
+      4216965: 0
       5623413: 0
+      7498942: 0
       10000000: 0
+      13335214: 0
       17782794: 0
+      23713737: 0
       31622777: 0
+      42169650: 0
       56234133: 0
+      74989421: 0
       100000000: 0
+      133352143: 0
       177827941: 0
+      237137371: 0
       316227766: 0
+      421696503: 0
       562341325: 0
+      749894209: 0
       1000000000: 0
     -+:
       0: 0
       1: 0
       2: 0
       3: 0
+      4: 0
       6: 0
+      7: 0
       10: 0
+      13: 0
       18: 0
+      24: 0
       32: 0
+      42: 0
       56: 0
+      75: 0
       100: 0
+      133: 0
       178: 0
+      237: 0
       316: 0
+      422: 0
       562: 0
+      750: 0
       1000: 0
+      1334: 0
       1778: 0
+      2371: 0
       3162: 0
+      4217: 0
       5623: 0
-      10000: 2
+      7499: 0
+      10000: 1
+      13335: 1
       17783: 0
-      31623: 1
+      23714: 0
+      31623: 0
+      42170: 1
       56234: 0
-      100000: 3
+      74989: 0
+      100000: 0
+      133352: 3
       177828: 0
+      237137: 0
       316228: 1
+      421697: 0
       562341: 0
+      749894: 0
       1000000: 0
+      1333521: 0
       1778279: 0
+      2371374: 0
       3162278: 0
+      4216965: 0
       5623413: 0
+      7498942: 0
       10000000: 0
+      13335214: 0
       17782794: 0
+      23713737: 0
       31622777: 0
+      42169650: 0
       56234133: 0
+      74989421: 0
       100000000: 0
+      133352143: 0
       177827941: 0
+      237137371: 0
       316227766: 0
+      421696503: 0
       562341325: 0
+      749894209: 0
       1000000000: 0
     --:
       0: 0
       1: 0
       2: 0
       3: 0
+      4: 0
       6: 0
+      7: 0
       10: 0
+      13: 0
       18: 0
+      24: 0
       32: 0
+      42: 0
       56: 0
+      75: 0
       100: 0
+      133: 0
       178: 0
+      237: 0
       316: 0
+      422: 0
       562: 0
+      750: 0
       1000: 1
-      1778: 1
+      1334: 0
+      1778: 0
+      2371: 1
       3162: 0
+      4217: 0
       5623: 0
+      7499: 0
       10000: 0
+      13335: 0
       17783: 0
-      31623: 21
+      23714: 0
+      31623: 0
+      42170: 21
       56234: 1
+      74989: 0
       100000: 2
+      133352: 0
       177828: 0
+      237137: 0
       316228: 0
+      421697: 0
       562341: 0
+      749894: 0
       1000000: 0
+      1333521: 0
       1778279: 0
+      2371374: 0
       3162278: 0
+      4216965: 0
       5623413: 0
+      7498942: 0
       10000000: 0
+      13335214: 0
       17782794: 0
+      23713737: 0
       31622777: 0
+      42169650: 0
       56234133: 0
+      74989421: 0
       100000000: 0
+      133352143: 0
       177827941: 0
+      237137371: 0
       316227766: 0
+      421696503: 0
       562341325: 0
+      749894209: 0
       1000000000: 0
     ++:
       0: 0
       1: 0
       2: 0
       3: 0
+      4: 0
       6: 0
+      7: 0
       10: 0
+      13: 0
       18: 0
+      24: 0
       32: 0
+      42: 0
       56: 0
+      75: 0
       100: 0
+      133: 0
       178: 0
+      237: 0
       316: 0
+      422: 0
       562: 0
+      750: 0
       1000: 0
+      1334: 0
       1778: 1
+      2371: 0
       3162: 0
+      4217: 0
       5623: 1
+      7499: 0
       10000: 1
+      13335: 0
       17783: 0
-      31623: 10
-      56234: 3
-      100000: 7
+      23714: 0
+      31623: 0
+      42170: 10
+      56234: 2
+      74989: 1
+      100000: 4
+      133352: 3
       177828: 2
+      237137: 0
       316228: 0
+      421697: 0
       562341: 0
+      749894: 0
       1000000: 0
+      1333521: 0
       1778279: 0
+      2371374: 0
       3162278: 0
+      4216965: 0
       5623413: 0
+      7498942: 0
       10000000: 0
+      13335214: 0
       17782794: 0
+      23713737: 0
       31622777: 0
+      42169650: 0
       56234133: 0
+      74989421: 0
       100000000: 0
+      133352143: 0
       177827941: 0
+      237137371: 0
       316227766: 0
+      421696503: 0
       562341325: 0
+      749894209: 0
       1000000000: 0
   chromsizes:
     Test_seq: 450060