Mercurial > repos > iuc > picrust2_pipeline
annotate picrust2_pipeline.xml @ 1:d6bb2e3febda draft default tip
planemo upload for repository https://github.com/picrust/picrust2 commit f8c32a316582ff102c9b7edf3817000691ef9eea
author | iuc |
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date | Tue, 13 Aug 2024 12:10:47 +0000 |
parents | 540aedfc81c7 |
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rev | line source |
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540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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1 <tool id="picrust2_pipeline" name="PICRUSt2 Full pipeline" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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2 <description></description> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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3 <macros> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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4 <import>macros.xml</import> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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5 </macros> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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6 <expand macro="bio_tool"/> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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7 <expand macro="requirements"/> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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8 <version_command>picrust2_pipeline.py -v</version_command> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
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9 <command detect_errors="exit_code"><![CDATA[ |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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10 @VAR_ACCESS_FOO@ |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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11 @PLACE_SEQS_PREPROCESSING@ |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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12 @PREPARE_METAGENOME_PIPELINE_PARAMS@ |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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13 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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14 picrust2_pipeline.py |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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15 ## place_seqs parameters |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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16 @PLACE_SEQS_PARAMS@ |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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17 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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18 ## hsp parameters |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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19 @HSP_PARAMS@ |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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20 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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21 ## metagenome_pipeline parameters |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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22 @METAGENOME_PIPELINE_PARAMS@ |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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23 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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24 ## pathway_pipeline parameters |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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25 #if $predict_pathways.no_pathways == '' |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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26 @PATHWAY_PIPELINE_PARAMS@ |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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27 --reaction_func '$predict_pathways.reaction_map_file.reaction_func' |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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28 #end if |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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29 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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30 #if $remove_intermediate is None |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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31 --remove_intermediate |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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32 #end if |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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33 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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34 --output 'full_pipeline_output' |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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35 --processes "\${GALAXY_SLOTS:-1}" |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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36 ## unzip all (potential) output files |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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37 && |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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38 find full_pipeline_output -name "*.gz" -exec gunzip {} \; |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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39 ## rename all tsv files to tabular to simplify discovery |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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40 && |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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41 find full_pipeline_output -name "*.tsv" -exec sh -c 'mv {} \$(dirname {})/\$(basename {} .tsv).tabular' \; |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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42 ## flatten metagenome output dirs ro simplify/enable discovery |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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43 && |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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44 for d in full_pipeline_output/*_metagenome_out; do |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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45 D=\$(basename \$d); |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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46 for i in \$d/*; do |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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47 I=\$(basename \$i); |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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48 mv "\$i" "full_pipeline_output/""\$D""_""\$I"; |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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49 done; |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
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50 done; |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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51 ]]></command> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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52 <inputs> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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53 <!-- place_seqs parameters --> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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54 <section name="place_seqs_section" title="Sequence placement options" expanded="true"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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55 <expand macro="place_seqs_params"/> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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56 </section> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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57 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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58 <!-- hsp parameters --> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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59 <section name="hsp_section" title="Hidden state prediction (HSP) options" expanded="true"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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60 <expand macro="hsp_params" nsti_falsevalue="--skip_nsti" nsti_checked="true" in_trait_arg="--in_traits" in_trait_multiple="true" in_trait_label_suff="(s)" in_traits_help="Note that EC numbers will always be predicted unless --no_pathways is set"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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61 <token name="custom_traits"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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62 <!-- both go separately into hsp observed_trait_table --> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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63 <param argument="--custom_trait_tables" type="data" multiple="true" format="tabular" label="Customized trait table(s)" help="with gene families as columns and genomes as rows"/> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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64 <!-- by default 16S is used also goes into observed_trait_table --> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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65 <param argument="--marker_gene_table" type="data" format="tabular" optional="true" label="Table of predicted marker gene (16S or other) copy numbers" /> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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66 </token> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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67 </expand> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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68 </section> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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69 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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70 <!-- metagenome_pipeline parameters --> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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71 <section name="metagenome_pipeline_section" title="Metagenome prediction options" expanded="true"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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72 <expand macro="metagenome_pipeline_params" stratified_arg="--stratified"/> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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73 </section> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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74 |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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75 <!-- pathway_pipeline parameters --> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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76 <conditional name="predict_pathways"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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77 <param argument="--no_pathways" type="select" truevalue="" falsevalue="--no_pathways" checked="true" label="Infer pathways"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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78 <option value="">Yes</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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79 <option value="--no_pathways">No</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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80 </param> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
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81 <when value=""> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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82 <expand macro="pathway_pipeline_params" mapargument="--pathway_map"/> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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83 <conditional name="reaction_map_file"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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84 <param name="selector" type="select" label="Functional database to use as reactions for inferring pathway abundances"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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85 <option value="default" selected="true">Default functional database</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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parents:
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86 <option value="custom">Customized reation functional map file</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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87 </param> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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88 <when value="default"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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89 <param argument="--reaction_func" type="select" label="Default functional database"> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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90 <option value="COG">Clusters of Orthologous Genes database (COG)</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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91 <option value="EC" selected="true">Enzyme Commission number database (EC number)</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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92 <option value="KO">KEGG Orthology database (KO)</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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93 <option value="PFAM">Pfam database</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
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94 <option value="TIGRFAM">TIGRFAM database</option> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
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95 </param> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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96 </when> |
540aedfc81c7
planemo upload for repository https://github.com/picrust/picrust2 commit 972784d909912af20cd213fc56830fee79d83ca6
iuc
parents:
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97 <when value="custom"> |
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98 <param argument="--reaction_func" type="data" format="tabular" label="Custom mapping table" help="An input 2 column map table linking function ids to descriptions for each function"/> |
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|
99 </when> |
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|
100 </conditional> |
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|
101 </when> |
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102 <when value="--no_pathways"/> |
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103 </conditional> |
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104 <param argument="--remove_intermediate" type="select" multiple="true" optional="true" label="Keep intermediate files of"> |
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105 <option value="place_seqs">place_seqs</option> |
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106 <option value="pathways">pathways_pipeline</option> |
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107 </param> |
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108 </inputs> |
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109 <outputs> |
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110 <!-- place seqs outputs --> |
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111 <expand macro="place_seqs_output" from_work_dir="full_pipeline_output/" label_suffix="(place_seqs)"> |
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112 <filter>remove_intermediate and "place_seqs" in remove_intermediate</filter> |
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113 </expand> |
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114 |
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115 <!-- hsp output --> |
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116 <collection name="hsp_output" type="list" label="${tool.name} on ${on_string}: predicted copy numbers" > |
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117 <discover_datasets pattern="(?P<designation>.+)_predicted\.tabular" directory="full_pipeline_output/" format="tabular"/> |
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118 </collection> |
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119 |
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120 <!-- metagenome output --> |
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121 <collection name="metagenome_output" type="list:list" label="${tool.name} on ${on_string}: metagenome output" > |
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122 <discover_datasets pattern="(?P<identifier_0>.+)_metagenome_out_(?P<identifier_1>.+)\.tabular" directory="full_pipeline_output/" format="tabular"/> |
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123 </collection> |
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124 |
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125 <!-- pathways output --> |
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126 <expand macro="pathways_output" from_work_dir="full_pipeline_output/" label_suffix="pathways"> |
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127 <filter>predict_pathways["no_pathways"] == ""</filter> |
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128 <token name="intermediate_filter"> |
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129 <filter>remove_intermediate and "pathways" in remove_intermediate</filter> |
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130 </token> |
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131 <token name="coverage_filter"> |
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132 <filter>predict_pathways["coverage"]</filter> |
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133 </token> |
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134 <token name="per_sequence_filter"> |
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135 <filter>predict_pathways["strat_output"]["per_sequence_contrib"]</filter> |
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136 </token> |
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137 </expand> |
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138 </outputs> |
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139 <tests> |
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140 <test expect_num_outputs="5"> |
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141 <!-- place_seq --> |
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142 <param name="study_fasta" value="study_seqs_full.fasta"/> |
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143 <param name="placement_tool" value="sepp"/> |
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144 <param name="min_align" value="0.0"/> |
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145 <!-- hsp --> |
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146 <conditional name="trait_input"> |
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147 <param name="selector" value="default"/> |
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148 <param name="in_traits" value="EC,COG"/> |
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149 </conditional> |
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150 <param name="skip_nsti" value="false"/> |
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151 <!-- metagenome --> |
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152 <param name="input" value="workflow_seq_abun.biom"/> |
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153 <conditional name="input_options"> |
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154 <param name="selector" value="ASV"/> |
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155 <param name="min_reads" value="1"/> |
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156 <param name="min_samples" value="1"/> |
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157 </conditional> |
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158 <param name="max_nsti" value="2.0"/> |
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159 <param name="skip_norm" value="false"/> |
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160 <!-- pathway --> |
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161 <param name="skip_minpath" value="false"/> |
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162 <param name="no_gap_fill" value="true"/> |
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163 |
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164 <param name="remove_intermediate" value="place_seqs"/> |
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165 <output name="out_tree" ftype="newick"> |
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166 <assert_contents> |
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167 <has_text text="643348582"/> |
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168 <has_n_lines n="1"/> |
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169 </assert_contents> |
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170 </output> |
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171 <output_collection name="place_seqs_intermediate_output" type="list" count="2"> |
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172 <element name="query_align" ftype="stockholm"> |
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173 <assert_contents> |
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174 <has_text text="STOCKHOLM 1.0"/> |
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175 <has_n_lines n="160106"/> |
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176 </assert_contents> |
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177 </element> |
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178 <element name="study_seqs_filtered" ftype="fasta"> |
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179 <assert_contents> |
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180 <has_text text="02905cfb87861c837dde629596d9272b"/> |
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181 <has_n_lines n="10"/> |
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182 </assert_contents> |
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183 </element> |
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184 </output_collection> |
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185 <output_collection name="hsp_output" type="list" count="3"> |
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186 <element name="COG"> |
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187 <assert_contents> |
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188 <has_text text="COG0001"/> |
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189 <has_n_lines n="6"/> |
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190 </assert_contents> |
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191 </element> |
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192 <element name="EC"> |
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193 <assert_contents> |
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194 <has_text text="EC:1.1.1.1"/> |
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195 <has_n_lines n="6"/> |
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196 </assert_contents> |
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197 </element> |
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198 <element name="marker"> |
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199 <assert_contents> |
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200 <has_text text="16S_rRNA_Count"/> |
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201 <has_n_lines n="6"/> |
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202 </assert_contents> |
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203 </element> |
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204 </output_collection> |
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205 <output_collection name="metagenome_output" type="list:list" count="2"> |
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206 <element name="COG"> |
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207 <element name="pred_metagenome_unstrat"> |
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208 <assert_contents> |
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209 <has_text text="function"/> |
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210 <has_n_lines n="1793"/> |
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211 </assert_contents> |
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212 </element> |
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213 <element name="seqtab_norm"> |
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214 <assert_contents> |
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215 <has_text text="normalized"/> |
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216 <has_n_lines n="6"/> |
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217 </assert_contents> |
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218 </element> |
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219 </element> |
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220 <element name="EC"> |
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221 <element name="pred_metagenome_unstrat"> |
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222 <assert_contents> |
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223 <has_text text="function"/> |
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224 <has_n_lines n="768"/> |
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225 </assert_contents> |
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226 </element> |
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227 <element name="seqtab_norm"> |
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228 <assert_contents> |
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229 <has_text text="normalized"/> |
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230 <has_n_lines n="6"/> |
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231 </assert_contents> |
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232 </element> |
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233 </element> |
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234 </output_collection> |
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235 <output name="pathways_output" ftype="tabular"> |
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236 <assert_contents> |
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237 <has_text text="pathway"/> |
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238 <has_n_lines n="149"/> |
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239 <has_n_columns n="3"/> |
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240 </assert_contents> |
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241 </output> |
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242 </test> |
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243 <!-- same as test 1, but only COG as in_traits (which has the same result since EC is added automatically), and test the other intermediate result --> |
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244 <test expect_num_outputs="5"> |
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245 <!-- place_seq --> |
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246 <param name="study_fasta" value="study_seqs_full.fasta"/> |
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247 <param name="placement_tool" value="sepp"/> |
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248 <param name="min_align" value="0.0"/> |
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249 <!-- hsp --> |
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250 <conditional name="trait_input"> |
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251 <param name="selector" value="default"/> |
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252 <param name="in_traits" value="COG"/> |
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253 </conditional> |
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254 <param name="skip_nsti" value="false"/> |
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255 <!-- metagenome --> |
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256 <param name="input" value="workflow_seq_abun.biom"/> |
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257 <conditional name="input_options"> |
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258 <param name="selector" value="ASV"/> |
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259 <param name="min_reads" value="1"/> |
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260 <param name="min_samples" value="1"/> |
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261 </conditional> |
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262 <param name="max_nsti" value="2.0"/> |
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263 <param name="skip_norm" value="false"/> |
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264 <!-- pathway --> |
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265 <param name="skip_minpath" value="false"/> |
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266 <param name="no_gap_fill" value="true"/> |
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267 |
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268 <param name="remove_intermediate" value="pathways"/> |
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269 <output name="out_tree" ftype="newick"> |
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270 <assert_contents> |
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271 <has_text text="643348582"/> |
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272 <has_n_lines n="1"/> |
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273 </assert_contents> |
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274 </output> |
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275 <output_collection name="hsp_output" type="list" count="3"> |
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276 <element name="COG" ftype="tabular"> |
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277 <assert_contents> |
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278 <has_text text="COG0001"/> |
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279 <has_n_lines n="6"/> |
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280 <has_n_columns n="4599"/> |
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281 </assert_contents> |
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282 </element> |
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283 <element name="EC" ftype="tabular"> |
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284 <assert_contents> |
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285 <has_text text="EC:1.1.1.1"/> |
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286 <has_n_lines n="6"/> |
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287 <has_n_columns n="2914"/> |
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288 </assert_contents> |
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289 </element> |
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290 <element name="marker" ftype="tabular"> |
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291 <assert_contents> |
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292 <has_text text="16S_rRNA_Count"/> |
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293 <has_n_lines n="6"/> |
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294 <has_n_columns n="2"/> |
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295 </assert_contents> |
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296 </element> |
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297 </output_collection> |
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298 |
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299 <output_collection name="metagenome_output" type="list:list" count="2"> |
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300 <element name="COG"> |
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301 <element name="pred_metagenome_unstrat" ftype="tabular"> |
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302 <assert_contents> |
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303 <has_text text="function"/> |
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304 <has_n_lines n="1793"/> |
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305 <has_n_columns n="3"/> |
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306 </assert_contents> |
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307 </element> |
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308 <element name="seqtab_norm" ftype="tabular"> |
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309 <assert_contents> |
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310 <has_text text="normalized"/> |
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311 <has_n_lines n="6"/> |
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312 <has_n_columns n="3"/> |
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313 </assert_contents> |
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314 </element> |
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315 </element> |
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316 <element name="EC"> |
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317 <element name="pred_metagenome_unstrat" ftype="tabular"> |
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318 <assert_contents> |
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319 <has_text text="function"/> |
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320 <has_n_lines n="768"/> |
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321 <has_n_columns n="3"/> |
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322 </assert_contents> |
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323 </element> |
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324 <element name="seqtab_norm" ftype="tabular"> |
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325 <assert_contents> |
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326 <has_text text="normalized"/> |
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327 <has_n_lines n="6"/> |
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328 <has_n_columns n="3"/> |
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329 </assert_contents> |
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330 </element> |
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331 </element> |
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332 </output_collection> |
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333 <output name="pathways_output" ftype="tabular"> |
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334 <assert_contents> |
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335 <has_text text="pathway"/> |
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336 <has_n_lines n="149"/> |
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337 <has_n_columns n="3"/> |
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338 </assert_contents> |
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339 </output> |
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340 <output_collection name="pathways_intermediate_output" type="list" count="1"> |
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341 <element name="regrouped_infile" ftype="tabular"> |
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342 <assert_contents> |
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343 <has_text text="function"/> |
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344 <has_n_lines n="1509"/> |
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345 <has_n_columns n="3"/> |
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346 </assert_contents> |
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347 </element> |
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348 </output_collection> |
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349 </test> |
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350 <!-- due to the long test times the other pathways_outputs |
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351 have been tested manually --> |
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352 </tests> |
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353 <help><![CDATA[ |
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354 @HELP_HEADER@ |
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355 |
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356 PICRUSt2 full pipeline |
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357 ====================== |
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358 Run sequence placement with EPA-NG and GAPPA to place study sequences (i.e. OTUs and ASVs) into a reference tree. Then runs hidden-state prediction with the castor R package to predict genome for each study sequence. Metagenome profiles are then generated, which can be optionally stratified by the contributing sequence. Finally, pathway abundances are predicted based on metagenome profiles. By default, output files include predictions for Enzyme Commission (EC) numbers, KEGG Orthologs (KOs), and MetaCyc pathway abundances. However, the tool enables users to use custom reference and trait tables to customize analyses. |
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359 |
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360 Note |
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361 ==== |
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362 The standard pipeline will generate metagenome predictions for 16S rRNA gene data. |
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363 |
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364 Input |
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365 ===== |
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366 1. A FASTA of amplicon sequences variants (ASVs; i.e. your representative sequences, not your raw reads) |
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367 2. A BIOM table of the abundance of each ASV across each sample. |
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368 |
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369 Output |
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370 ====== |
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371 1. Output tree with placed study sequences. |
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372 2. Metagenome Predictions |
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373 3. Pathway level predictions |
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374 |
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375 ]]></help> |
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376 <citations> |
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377 <citation type="doi">10.1038/s41587-020-0548-6</citation> |
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378 </citations> |
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379 </tool> |