Mercurial > repos > iuc > pmids_to_pubtator_matrix
annotate pubmed_by_queries.R @ 0:69714f06f18b draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
author | iuc |
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date | Wed, 24 Mar 2021 08:33:56 +0000 |
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0
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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1 #!/usr/bin/env Rscript |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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2 #tool: pubmed_by_queries |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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3 # |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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4 #This tool uses a set of search queries to download a defined number of abstracts or |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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5 #PMIDs for search query from PubMed. PubMed's search rules and syntax apply. |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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6 # |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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7 #Input: Tab-delimited table with search queries in a column starting with "ID_", |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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8 #e.g. "ID_gene" if search queries are genes. |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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9 # |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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10 #Output: Input table with additional columns |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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11 #with PMIDs or abstracts (--abstracts) from PubMed. |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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12 # |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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13 #Usage: |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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14 #$pubmed_by_queries.R [-h] [-i INPUT] [-o OUTPUT] [-n NUMBER] [-a] [-k KEY] |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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15 # |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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16 #optional arguments: |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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17 # -h, --help show this help message and exit |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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18 # -i INPUT, --input INPUT input file name. add path if file is not in working directory |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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19 # -o OUTPUT, --output OUTPUT output file name. [default "pubmed_by_queries_output"] |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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20 # -n NUMBER, --number NUMBER number of PMIDs or abstracts to save per ID [default "5"] |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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21 # -a, --abstract if abstracts instead of PMIDs should be retrieved use --abstracts |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
iuc
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22 # -k KEY, --key KEY if ncbi API key is available, add it to speed up the download of PubMed data. |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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23 # For usage in Galaxy add the API key to the Galaxy user-preferences (User/ Preferences/ Manage Information). |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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24 |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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25 if ("--install_packages" %in% commandArgs()) { |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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26 print("Installing packages") |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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27 if (!require("argparse")) install.packages("argparse", repo = "http://cran.rstudio.com/") ; |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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28 if (!require("easyPubMed")) install.packages("easyPubMed", repo = "http://cran.rstudio.com/") ; |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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29 } |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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30 |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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31 suppressPackageStartupMessages(library("argparse")) |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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32 suppressPackageStartupMessages(library("easyPubMed")) |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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33 |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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34 parser <- ArgumentParser() |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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35 parser$add_argument("-i", "--input", |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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36 help = "Input fie name. add path if file is not in working directory") |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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37 parser$add_argument("-o", "--output", default = "pubmed_by_queries_output", |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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38 help = "Output file name. [default \"%(default)s\"]") |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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39 parser$add_argument("-n", "--number", type = "integer", default = 5, |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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40 help = "Number of PMIDs (or abstracts) to save per ID. [default \"%(default)s\"]") |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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41 parser$add_argument("-a", "--abstract", action = "store_true", default = FALSE, |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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42 help = "If abstracts instead of PMIDs should be retrieved use --abstracts ") |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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43 parser$add_argument("-k", "--key", type = "character", |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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44 help = "If ncbi API key is available, add it to speed up the download of PubMed data. For usage in Galaxy add the API key to the Galaxy user-preferences (User/ Preferences/ Manage Information).") |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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45 parser$add_argument("--install_packages", action = "store_true", default = FALSE, |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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46 help = "If you want to auto install missing required packages.") |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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47 args <- parser$parse_args() |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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48 |
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49 if (!is.null(args$key)) { |
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50 if (file.exists(args$key)) { |
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51 credentials <- read.table(args$key, quote = "\"", comment.char = "") |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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52 args$key <- credentials[1, 1] |
69714f06f18b
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53 } |
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54 } |
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55 |
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56 max_web_tries <- 100 |
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57 |
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58 data <- read.delim(args$input, stringsAsFactors = FALSE) |
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59 |
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60 id_col_index <- grep("ID_", names(data)) |
69714f06f18b
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61 |
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62 |
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63 fetch_pmids <- function(data, number, pubmed_search, query, row, max_web_tries) { |
69714f06f18b
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64 my_pubmed_url <- paste("https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi?", |
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65 "db=pubmed&retmax=", number, |
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66 "&term=", pubmed_search$OriginalQuery, |
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67 "&usehistory=n", sep = "") |
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68 # get ids |
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69 idxml <- c() |
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70 for (i in seq(max_web_tries)) { |
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71 tryCatch({ |
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72 id_connect <- suppressWarnings(url(my_pubmed_url, open = "rb", encoding = "UTF8")) |
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73 idxml <- suppressWarnings(readLines(id_connect, warn = FALSE, encoding = "UTF8")) |
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74 suppressWarnings(close(id_connect)) |
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75 break |
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76 }, error = function(e) { |
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77 print(paste("Error getting URL, sleeping", 2 * i, "seconds.")) |
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78 print(e) |
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79 Sys.sleep(time = 2 * i) |
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80 }) |
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81 } |
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82 pmids <- c() |
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83 for (i in seq(length(idxml))) { |
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84 if (grepl("^<Id>", idxml[i])) { |
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85 pmid <- custom_grep(idxml[i], tag = "Id", format = "char") |
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86 pmids <- c(pmids, as.character(pmid[1])) |
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87 } |
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88 } |
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89 if (length(pmids) > 0) { |
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90 data[row, sapply(seq(length(pmids)), function(i) { |
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91 paste0("PMID_", i) |
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92 })] <- pmids |
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93 cat(length(pmids), " PMIDs for ", query, " are added in the table.", "\n") |
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94 } |
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95 return(data) |
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96 } |
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97 |
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98 |
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99 fetch_abstracts <- function(data, number, query, pubmed_search) { |
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100 efetch_url <- paste("https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?", |
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101 "db=pubmed&WebEnv=", pubmed_search$WebEnv, "&query_key=", pubmed_search$QueryKey, |
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102 "&retstart=", 0, "&retmax=", number, |
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103 "&rettype=", "null", "&retmode=", "xml", sep = "") |
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104 api_key <- pubmed_search$APIkey |
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105 if (!is.null(api_key)) { |
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parents:
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106 efetch_url <- paste(efetch_url, "&api_key=", api_key, sep = "") |
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107 } |
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108 # initialize |
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109 out_data <- NULL |
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110 try_num <- 1 |
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111 t_0 <- Sys.time() |
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112 # Try to fetch results |
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113 while (is.null(out_data)) { |
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114 # Timing check: kill at 3 min |
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115 if (try_num > 1) { |
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116 Sys.sleep(time = 2 * try_num) |
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117 cat("Problem to receive PubMed data or error is received. Please wait. Try number:", |
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118 try_num, "\n") |
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119 } |
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120 t_1 <- Sys.time() |
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121 if (as.numeric(difftime(t_1, t_0, units = "mins")) > 3) { |
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122 message("Killing the request! Something is not working. Please, try again later", |
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123 "\n") |
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124 return(data) |
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125 } |
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126 # ENTREZ server connect |
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127 out_data <- tryCatch({ |
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128 tmp_connect <- suppressWarnings(url(efetch_url, |
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129 open = "rb", |
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130 encoding = "UTF8")) |
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131 suppressWarnings(readLines(tmp_connect, |
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132 warn = FALSE, |
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133 encoding = "UTF8")) |
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134 }, error = function(e) { |
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135 print(e) |
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136 }, finally = { |
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137 try(suppressWarnings(close(tmp_connect)), |
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138 silent = TRUE) |
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139 }) |
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140 # Check if error |
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141 if (!is.null(out_data) && |
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142 class(out_data) == "character" && |
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143 grepl("<ERROR>", substr(paste(utils::head(out_data, n = 100), |
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144 collapse = ""), 1, 250))) { |
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145 out_data <- NULL |
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146 } |
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147 try_num <- try_num + 1 |
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148 } |
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149 if (is.null(out_data)) { |
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150 message("Killing the request! Something is not working. Please, try again later", |
69714f06f18b
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151 "\n") |
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152 return(data) |
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153 } else { |
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154 return(out_data) |
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155 } |
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iuc
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156 } |
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157 |
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158 |
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159 process_xml_abstracts <- function(out_data) { |
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160 xml_data <- paste(out_data, collapse = "") |
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161 # articles to list |
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162 xml_data <- strsplit(xml_data, "<PubmedArticle(>|[[:space:]]+?.*>)")[[1]][-1] |
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163 xml_data <- sapply(xml_data, function(x) { |
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164 #trim extra stuff at the end of the record |
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165 if (!grepl("</PubmedArticle>$", x)) |
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166 x <- sub("(^.*</PubmedArticle>).*$", "\\1", x) |
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167 # Rebuid XML structure and proceed |
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168 x <- paste("<PubmedArticle>", x) |
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169 gsub("[[:space:]]{2,}", " ", x) |
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170 }, |
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171 USE.NAMES = FALSE, simplify = TRUE) |
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172 #titles |
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173 titles <- sapply(xml_data, function(x) { |
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174 x <- custom_grep(x, tag = "ArticleTitle", format = "char") |
69714f06f18b
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175 x <- gsub("</{0,1}i>", "", x, ignore.case = T) |
69714f06f18b
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176 x <- gsub("</{0,1}b>", "", x, ignore.case = T) |
69714f06f18b
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177 x <- gsub("</{0,1}sub>", "", x, ignore.case = T) |
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178 x <- gsub("</{0,1}exp>", "", x, ignore.case = T) |
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179 if (length(x) > 1) { |
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180 x <- paste(x, collapse = " ", sep = " ") |
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181 } else if (length(x) < 1) { |
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182 x <- NA |
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183 } |
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184 x |
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185 }, |
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186 USE.NAMES = FALSE, simplify = TRUE) |
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187 # abstracts |
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188 abstract_text <- sapply(xml_data, function(x) { |
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189 custom_grep(x, tag = "AbstractText", format = "char") |
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190 }, |
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191 USE.NAMES = FALSE, simplify = TRUE) |
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192 abstracts <- sapply(abstract_text, function(x) { |
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193 if (length(x) > 1) { |
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194 x <- paste(x, collapse = " ", sep = " ") |
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195 x <- gsub("</{0,1}i>", "", x, ignore.case = T) |
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196 x <- gsub("</{0,1}b>", "", x, ignore.case = T) |
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197 x <- gsub("</{0,1}sub>", "", x, ignore.case = T) |
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198 x <- gsub("</{0,1}exp>", "", x, ignore.case = T) |
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199 } else if (length(x) < 1) { |
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200 x <- NA |
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201 } else { |
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202 x <- gsub("</{0,1}i>", "", x, ignore.case = T) |
69714f06f18b
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203 x <- gsub("</{0,1}b>", "", x, ignore.case = T) |
69714f06f18b
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204 x <- gsub("</{0,1}sub>", "", x, ignore.case = T) |
69714f06f18b
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205 x <- gsub("</{0,1}exp>", "", x, ignore.case = T) |
69714f06f18b
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206 } |
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207 x |
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208 }, |
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209 USE.NAMES = FALSE, simplify = TRUE) |
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210 #add title to abstracts |
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211 if (length(titles) == length(abstracts)) { |
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212 abstracts <- paste(titles, abstracts) |
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213 } |
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214 return(abstracts) |
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215 } |
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216 |
69714f06f18b
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217 |
69714f06f18b
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218 pubmed_data_in_table <- function(data, row, query, number, key, abstract) { |
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219 if (is.null(query)) { |
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220 print(data) |
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221 } |
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222 pubmed_search <- get_pubmed_ids(query, api_key = key) |
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223 if (as.numeric(pubmed_search$Count) == 0) { |
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224 cat("No PubMed result for the following query: ", query, "\n") |
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225 return(data) |
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226 } else if (abstract == FALSE) { # fetch PMIDs |
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227 data <- fetch_pmids(data, number, pubmed_search, query, row, max_web_tries) |
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228 return(data) |
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229 } else if (abstract == TRUE) { # fetch abstracts and title text |
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230 out_data <- fetch_abstracts(data, number, query, pubmed_search) |
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231 abstracts <- process_xml_abstracts(out_data) |
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232 #add abstracts to data frame |
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233 if (length(abstracts) > 0) { |
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234 data[row, sapply(seq(length(abstracts)), |
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235 function(i) { |
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236 paste0("ABSTRACT_", i) |
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237 })] <- abstracts |
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238 cat(length(abstracts), " abstracts for ", query, " are added in the table.", |
69714f06f18b
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239 "\n") |
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240 } |
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241 return(data) |
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242 } |
69714f06f18b
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243 } |
69714f06f18b
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244 |
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245 for (i in seq(nrow(data))) { |
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246 data <- tryCatch(pubmed_data_in_table(data = data, |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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247 row = i, |
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248 query = data[i, id_col_index], |
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249 number = args$number, |
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250 key = args$key, |
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251 abstract = args$abstract), error = function(e) { |
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252 print("main error") |
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253 print(e) |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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254 Sys.sleep(5) |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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255 }) |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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256 } |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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257 |
69714f06f18b
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tools/simtext commit 63a5e13cf89cdd209d20749c582ec5b8dde4e208"
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258 write.table(data, args$output, append = FALSE, sep = "\t", row.names = FALSE, col.names = TRUE, quote = FALSE) |