Mercurial > repos > iuc > pygenometracks
diff pyGenomeTracks.xml @ 0:2074627b0f83 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/pygenometracks commit a68062b7e444233416bd30404be9bf92b6f1a363
author | iuc |
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date | Sat, 23 Jun 2018 02:28:35 -0400 |
parents | |
children | 0ea86f6bf2a1 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pyGenomeTracks.xml Sat Jun 23 02:28:35 2018 -0400 @@ -0,0 +1,768 @@ +<tool id="pygenomeTracks" name="@BINARY@" version="@WRAPPER_VERSION@.0"> + <description>plot genomic data tracks</description> + <macros> + <token name="@BINARY@">pyGenomeTracks</token> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <command detect_errors="exit_code"> +<![CDATA[ + + sed '/^$/d' '$tracks_config' && + @BINARY@ + --tracks '$tracks_config' + #if $region and $region is not None: + --region '$region' + #end if + --outFileName plot.$image_file_format + && mv plot.$image_file_format plot +]]> + </command> + <configfiles> + <configfile name="tracks_config"> +#if $x_axis.x_axis_select == "yes": +[x-axis] +#if $x_axis.fontsize: +fontsize = $x_axis.fontsize +#end if +#if $x_axis.where: +where = $x_axis.where +#end if +#end if +#for $counter, $track in enumerate($tracks): +#if $track.track_file_style_conditional.track_file_style_selector == "hic_matrix_option": + +#for $counter_matrix, $data_matrix in enumerate($track.track_file_style_conditional.matrix_h5_cooler_multiple): +[hic_section_$counter] +file = $data_matrix +file_type = hic_matrix +#if $track.track_file_style_conditional.title: +title = $track.track_file_style_conditional.title +#else: +title = $data_matrix.name +#end if +#if $track.track_file_style_conditional.depth: +depth = $track.track_file_style_conditional.depth +#end if +#if $track.track_file_style_conditional.colormap: +colormap = $track.track_file_style_conditional.colormap +#end if +#if $track.track_file_style_conditional.min_value: +min_value = $track.track_file_style_conditional.min_value +#end if +#if $track.track_file_style_conditional.max_value: +max_value = $track.track_file_style_conditional.max_value +#end if +transform = $track.track_file_style_conditional.transform +height = $track.track_file_style_conditional.height_matrix + +#if $track.track_file_style_conditional.boundaries_file is not None: +#set boundary_file = $track.track_file_style_conditional.boundaries_file[$counter_matrix] +boundaries_file = $boundary_file +#end if + +#if $track.track_file_style_conditional.x_labels: +x labels = $track.track_file_style_conditional.x_labels +#end if +#if $track.track_file_style_conditional.type: +type = $track.track_file_style_conditional.type +#end if +#if $track.track_file_style_conditional.show_masked_bins: +show_masked_bins = $track.track_file_style_conditional.show_masked_bins +#end if +#if $track.track_file_style_conditional.scale_factor: +scale factor = $track.track_file_style_conditional.scale_factor +#end if + +[spacer] +#if $track.track_file_style_conditional.spacer_height: +height = $track.track_file_style_conditional.spacer_height +#end if +#end for + +#end if + + + +#if $track.track_file_style_conditional.track_file_style_selector == "tad_score_track_option": + +#for $data_bedgraph in $track.track_file_style_conditional.track_input_bedgraph: +[tad_score_$counter] +file = $data_bedgraph +#if $track.track_file_style_conditional.title: +title = $track.track_file_style_conditional.title +#else: +title = $data_bedgraph.name +#end if +#if $track.track_file_style_conditional.color: +color = $track.track_file_style_conditional.color +#end if +#if $track.track_file_style_conditional.height_tad: +height = $track.track_file_style_conditional.height_tad +#end if +#if $track.track_file_style_conditional.type_lines: +type = $track.track_file_style_conditional.type_lines +#end if +#if $track.track_file_style_conditional.show_data: +show data range = $track.track_file_style_conditional.show_data +#end if + +#set $columns = len(open(str($track.track_file_style_conditional.track_input_bedgraph)).readline().split('\t')) +#if $columns > 5: +file_type = bedgraph_matrix +#else: +file_type = bedgraph +#end if +type = lines +[spacer] +#if $track.track_file_style_conditional.spacer_height: +height = $track.track_file_style_conditional.spacer_height +#end if +#end for +#end if + + + + +#if $track.track_file_style_conditional.track_file_style_selector == "chrom_states_option": +#for $data_bed in $track.track_file_style_conditional.track_input_bed: + +[chrom states_$counter] +file = $data_bed +#if $track.track_file_style_conditional.title: +title = $track.track_file_style_conditional.title +#else: +title = $data_bed.name +#end if +#if $track.track_file_style_conditional.color: +color = $track.track_file_style_conditional.color +#end if +display = collapsed +#if $track.track_file_style_conditional.height_chrom: +height = $track.track_file_style_conditional.height_chrom +#end if +#if $track.track_file_style_conditional.border_color: +border_color = $track.track_file_style_conditional.border_color +#end if +file_type = bed +[spacer] +#if $track.track_file_style_conditional.spacer_height: +height = $track.track_file_style_conditional.spacer_height +#end if +#end for + +#end if + + + + +#if $track.track_file_style_conditional.track_file_style_selector == "gene_track_option": +#for $data_bed in $track.track_file_style_conditional.track_input_bed: + +[genes_$counter] +file = $data_bed +#if $track.track_file_style_conditional.title: +title = $track.track_file_style_conditional.title +#else: +title = $data_bed.name +#end if +#if $track.track_file_style_conditional.color: +color = $track.track_file_style_conditional.color +#end if + +#if $track.track_file_style_conditional.height_bed: +height = $track.track_file_style_conditional.height_bed +#end if + +#if $track.track_file_style_conditional.labels: +labels = $track.track_file_style_conditional.labels +#end if + +#if $track.track_file_style_conditional.type: +type = $track.track_file_style_conditional.type +#end if + +file_type = bed +#if $track.track_file_style_conditional.fontsize: +fontsize = $track.track_file_style_conditional.fontsize +#end if + +#if $track.track_file_style_conditional.gene_rows: +gene rows = $track.track_file_style_conditional.gene_rows +#end if + +#if $track.track_file_style_conditional.global_max_row: +global max row = $track.track_file_style_conditional.global_max_row +#end if +[spacer] +#if $track.track_file_style_conditional.spacer_height: +height = $track.track_file_style_conditional.spacer_height +#end if +#end for +#end if + + +#if $track.track_file_style_conditional.track_file_style_selector == "bedgraph_track_option": +#for $data_bedgraph in $track.track_file_style_conditional.track_input_bedgraph: + +[bedgraph_$counter] +file = $data_bedgraph +#if $track.track_file_style_conditional.title: +title = $track.track_file_style_conditional.title +#else: +title = $data_bedgraph.name +#end if +#if $track.track_file_style_conditional.color: +color = $track.track_file_style_conditional.color +#end if +#if $track.track_file_style_conditional.height_bedgraph: +height = $track.track_file_style_conditional.height_bedgraph +#end if +#if $track.track_file_style_conditional.show_data: +show data range = $track.track_file_style_conditional.show_data +#end if + +#set $columns = len(open(str($track.track_file_style_conditional.track_input_bedgraph)).readline().split('\t')) +#if $columns > 5: +file_type = bedgraph_matrix +#else: +file_type = bedgraph +#end if +type = lines +[spacer] +#if $track.track_file_style_conditional.spacer_height: +height = $track.track_file_style_conditional.spacer_height +#end if +#end for +#end if + +#if $track.track_file_style_conditional.track_file_style_selector == "bigwig_track_option": +#for $data_bigwig in $track.track_file_style_conditional.track_input_bigwig: +[bigwig_$counter] +file = $data_bigwig +#if $track.track_file_style_conditional.title: +title = $track.track_file_style_conditional.title +#else: +title = $data_bigwig.name +#end if +color = $track.track_file_style_conditional.color +#if $track.track_file_style_conditional.min_value: +min_value = $track.track_file_style_conditional.min_value +#else: +min_value = 0 +#end if +#if $track.track_file_style_conditional.max_value: +max_value = $track.track_file_style_conditional.max_value +#else: +max_value = auto +#end if +height = $track.track_file_style_conditional.height_bigwig +#if $track.track_file_style_conditional.number_of_bins: +number of bins = $track.track_file_style_conditional.number_of_bins +#end if +nans to zeros = $track.track_file_style_conditional.nans_to_zeros +#if $track.track_file_style_conditional.type_conditional == 'fill_option': +type = fill +#elif $track.track_file_style_conditional.type_conditional == 'line_option': +type = line:$track.track_file_style_conditional.type_conditional.height_line +#elif $track.track_file_style_conditional.type_conditional == 'point_option': +type = points:$track.track_file_style_conditional.type_conditional.width_point +#end if +#if $track.track_file_style_conditional.data_range: +show data range = $track.track_file_style_conditional.data_range +#end if +file_type = bigwig +[spacer] +#if $track.track_file_style_conditional.spacer_height: +height = $track.track_file_style_conditional.spacer_height +#end if +#end for +#end if + + +#if $track.track_file_style_conditional.track_file_style_selector == "bedgraph_matrix_track_option": +#for $data_bedgraph_matrix in $track.track_file_style_conditional.track_input_bedgraph_matrix: + +[bedgraph_matrix_$counter] +file = $data_bedgraph_matrix +#if $track.track_file_style_conditional.title: +title = $track.track_file_style_conditional.title +#else: +title = $data_bedgraph_matrix.name +#end if +#if $track.track_file_style_conditional.height_bedgraph: +height = $track.track_file_style_conditional.height_bedgraph +#end if +orientation = inverted +#if $track.track_file_style_conditional.min_value: +min_value = $track.track_file_style_conditional.min_value +#end if +#if $track.track_file_style_conditional.max_value: +max_value = $track.track_file_style_conditional.max_value +#end if +#if $track.track_file_style_conditional.type_lines: +type = $track.track_file_style_conditional.type_lines +#end if +file_type = bedgraph_matrix +plot horizontal lines=False +[spacer] +#if $track.track_file_style_conditional.spacer_height: +height = $track.track_file_style_conditional.spacer_height +#end if +#end for +#end if + +#if $track.track_file_style_conditional.track_file_style_selector == "vlines_track_option": + +[vlines_$counter] +file = $track.track_file_style_conditional.track_input_bed_single +type = vlines +file_type = bed +#end if +#if $track.track_file_style_conditional.track_file_style_selector == "spacer_option": + +[spacer] +#if $track.track_file_style_conditional.spacer_height: +height = $track.track_file_style_conditional.spacer_height +#end if +#end if +#end for</configfile> + </configfiles> + <inputs> + <expand macro="region" /> + + <repeat name="tracks" min="1" title="Include tracks in your plot" + help="Tracks can be of different filetypes. E.g BED, HiC-Matrix, BigWig or BedGraph"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" type="select" label="Choose style of the track"> + <option value="hic_matrix_option">TAD visualization</option> + <option value="chrom_states_option">Chromatine states</option> + <option value="tad_score_track_option">TAD score</option> + <option value="gene_track_option">Gene track / Bed track</option> + <option value="bigwig_track_option">Bigwig track</option> + <option value="bedgraph_track_option">Bedgraph track </option> + <option value="bedgraph_matrix_track_option">Bedgraph matrix track</option> + <option value="vlines_track_option">Vlines track</option> + <option value="spacer_option">Spacer</option> + </param> + <when value="hic_matrix_option"> + <expand macro="plot_title" /> + <expand macro="matrix_h5_cooler_multiple_macro" /> + + <expand macro="colormap" /> + <param name="depth" type="integer" value="8000000" optional="True" label="Depth" /> + <param name="min_value" type="float" value="" optional="True" label="Minimum value"/> + <param name="max_value" type="float" value="" optional="True" label="Maximum value"/> + <param name="transform" type="select" label="Plot the transformed value"> + <option value="log1p" selected="True" >log1p</option> + <option value="log">log</option> + <option value="-log">-log</option> + + </param> + <param name="height_matrix" type="float" value="1.5" optional="True" label="height"/> + + <param name="type" type="select" optional="True" label="Plotting type"> + <option value="arcplot">arcplot</option> + <option value="interaction">interaction</option> + </param> + <param name="show_masked_bins" type="boolean" truevalue="yes" falsevalue="no" checked="false" + label="Show masked bins" /> + <param name="boundaries_file" type="data" optional="True" format="bed" label="Boundaries file" multiple='True'/> <!-- multiple='True'--> + <param name="scale_factor" type="float" value="1.0" optional="True" label="Scale factor" help="if the values in the matrix need to be scaled the following parameter can be used" /> + <param name="x_labels" type="boolean" truevalue="yes" falsevalue="no" optional="True" label="Show x labels" /> + <expand macro="spacer_macro" /> + + </when> + <when value="tad_score_track_option"> + <expand macro="plot_title" /> + <expand macro="track_input_bedgraph_macro" /> + <param name="color" type="color" value="#000000" label="Color of track" optional="True" /> + <param name="type_lines" type="boolean" truevalue="lines" falsevalue="" checked="false" + label="Set 'type' to 'lines'" help="if type is set as lines, then the TAD score lines are drawn instead + of the matrix set to lines if a heatmap representing the matrix is not wanted"/> + <param name="height_tad" type="float" value="0.2" optional="True" label="height"/> + <param name='show_data' type="boolean" truevalue="yes" falsevalue="no" checked="false" optional="True" label="Show visualization of data range" /> + <expand macro="spacer_macro" /> + + </when> + <when value="chrom_states_option"> + <expand macro="plot_title" /> + + <expand macro="track_input_bed_macro" /> + <param name="color" type="color" value="#000000" label="Color of track" optional="True" /> + + <param name="border_color" type="color" value="#000000" label="Border color" optional="True" /> + <param name="height_chrom" type="float" value="1.5" optional="True" label="height"/> + <param name="labels" type="boolean" truevalue="on" falsevalue="off" checked="true" + label="Plot labels" /> + <expand macro="spacer_macro" /> + + </when> + <when value="gene_track_option"> + <expand macro="plot_title" /> + + <expand macro="track_input_bed_macro" /> + <param name="color" type="color" value="#000000" label="Color of track" optional="True" /> + <param name="height_bed" type="float" value="1.5" optional="True" label="height"/> + <param name="labels" type="boolean" truevalue="on" falsevalue="off" checked="true" + label="Plot labels" /> + <param name="type" type="select" optional="True" label="Type"> + <option value="genes">genes</option> + <option value="domains">domains</option> + </param> + <param name="global_max_row" type="boolean" truevalue="yes" falsevalue="no" checked="false" + label="Global max rows" /> + <param name="gene_rows" type="integer" value="" optional="True" label="Gene rows" /> + <param name="fontsize" type="integer" value="" optional="True" label="Fontsize" /> + <expand macro="spacer_macro" /> + + </when> + <when value="bedgraph_track_option"> + <expand macro="plot_title" /> + <expand macro="track_input_bedgraph_macro" /> + <param name="color" type="color" value="#000000" label="Color of track" optional="True" /> + + <param name="height_bedgraph" type="float" value="0.2" optional="True" label="height"/> + <param name='show_data' type="boolean" truevalue="yes" falsevalue="no" checked="false" optional="True" label="Show visualization of data range" /> + <expand macro="spacer_macro" /> + + </when> + <when value="bigwig_track_option"> + <expand macro="plot_title" /> + + <expand macro="track_input_bigwig_macro" /> + <param name="color" type="color" value="#000000" label="Color of track" optional="True" /> + + <param name="min_value" type="float" value="" optional="True" label="Minimum value"/> + <param name="max_value" type="float" value="" optional="True" label="Maximum value"/> + + <param name="height_bigwig" type="float" value="1.5" optional="True" label="height"/> + <param name="number_of_bins" type="integer" value="" optional="True" label="Number of bins" /> + <param name="nans_to_zeros" type="boolean" truevalue="True" falsevalue="False" checked="false" + label="NAN's to zeros" /> + <conditional name="type_conditional"> + <param name="type_selector" type="select" label="Choose style of the plot."> + <option value="fill_option">fill</option> + <option value="line_option">line</option> + <option value="point_option">point</option> + </param> + <when value="line_option"> + <param name="width_line" type="float" value="0.5" optional="True" label="Width"/> + </when> + <when value="point_option"> + <param name="width_point" type="float" value="0.5" optional="True" label="Width"/> + </when> + <when value="fill_option" /> + </conditional> + <param name="data_range" type="boolean" truevalue="yes" falsevalue="no" optional="True" checked="false" /> + <expand macro="spacer_macro" /> + + </when> + + + <when value="bedgraph_matrix_track_option"> + <expand macro="plot_title" /> + + <expand macro="track_input_bedgraph_matrix_macro" /> + <param name="min_value" type="float" value="" optional="True" label="Minimum value"/> + <param name="max_value" type="float" value="" optional="True" label="Maximum value"/> + + <param name="height_bedgraph" type="float" value="1.5" optional="True" label="height"/> + + <param name="type_lines" type="boolean" truevalue="lines" falsevalue="" checked="false" + label="Set 'type' to 'lines'" help="if type is set as lines, then the TAD score lines are drawn instead + of the matrix set to lines if a heatmap representing the matrix is not wanted"/> + <expand macro="spacer_macro" /> + + </when> + <when value="vlines_track_option"> + <expand macro="track_input_bed_single_macro" /> + </when> + <when value="spacer_option"> + <expand macro="spacer_macro" /> + </when> + </conditional> + + </repeat> + + + <conditional name="x_axis"> + <param name="x_axis_select" type="select" label="Configure x-axis"> + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + <when value="yes"> + <param name="fontsize" type="integer" value="" optional="True" label="Fontsize" /> + <param name="where" type="select" optional="True" label="Where to place the x-axis"> + <option value="top">Top</option> + <option value="bottom">Bottom</option> + </param> + </when> + <when value="no" /> + </conditional> + <param name="image_file_format" type="select" label="Image output format"> + <option value="png">png</option> + <option value="svg">svg</option> + </param> + </inputs> + <outputs> + <data format="png" name="outFileName" from_work_dir="plot" label="${tool.name} on [${on_string}]: Plot"> + <change_format> + <when input="image_file_format" value="png" format="png" /> + <when input="image_file_format" value="svg" format="svg" /> + </change_format> + </data> + </outputs> + <tests> + <test> + <param name="region" value="chrX:3000000-3500000"/> + <conditional name="x_axis"> + <param name="x_axis_select" value="yes" /> + </conditional> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="hic_matrix_option" /> + <param name="matrix_h5_cooler_multiple" value="Li_et_al_2015.h5"/> + + <param name="title" value="Kc DpnII (Li et al. 2015)" /> + <param name="colormap" value="RdYlBu_r" /> + <param name="depth" value="200000" /> + <param name="transform" value="log1p" /> + <param name="boundaries_file" value="domains.bed" /> + <param name="x_labels" value="True" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="spacer_option" /> + <param name="spacer_height" value="0.05" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="chrom_states_option" /> + <param name="track_input_bed" value="tad_classification.bed" ftype="bed" /> + <param name="title" value="TAD state" /> + <param name="height_chrom" value="0.5" /> + <param name="labels" value="off" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="tad_score_track_option" /> + <param name="track_input_bedgraph" value="tad_score.gz" ftype="bedgraph" /> + <param name="title" value="TAD separation score (Ramirez et al.)" /> + <param name="height_tad" value="10" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="spacer_option" /> + <param name="spacer_height" value="1" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="bedgraph_track_option" /> + <param name="track_input_bedgraph" value="bedgraph_chrx_2e6_5e6.bg" ftype="bedgraph" /> + <param name="title" value="bedgraph" /> + <param name="height_bedgraph" value="4" /> + <param name="color" value="blue" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="bigwig_track_option" /> + <param name="track_input_bigwig" value="bigwig_chrx_2e6_5e6.bw" ftype="bigwig" /> + <param name="title" value="rep 1 test fill" /> + <param name="height_bigwig" value="4" /> + <param name="color" value="blue" /> + <conditional name="type_conditional"> + <param name="type_selector" value="fill_option" /> + </conditional> + </conditional> + </repeat> + + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="bigwig_track_option" /> + <param name="track_input_bigwig" value="bigwig_chrx_2e6_5e6.bw" ftype="bigwig" /> + <param name="title" value="rep 1 test line" /> + <param name="height_bigwig" value="4" /> + <param name="color" value="red" /> + <conditional name="type_conditional"> + <param name="type_selector" value="line_option" /> + <param name="width_line" value="1" /> + </conditional> + </conditional> + </repeat> + + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="bigwig_track_option" /> + <param name="track_input_bigwig" value="bigwig_chrx_2e6_5e6.bw" ftype="bigwig" /> + <param name="title" value="rep 1 test lw=0.2" /> + <param name="height_bigwig" value="4" /> + <param name="color" value="red" /> + <conditional name="type_conditional"> + <param name="type_selector" value="line_option" /> + <param name="width_line" value="0.1" /> + + </conditional> + </conditional> + </repeat> + + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="bigwig_track_option" /> + <param name="track_input_bigwig" value="bigwig_chrx_2e6_5e6.bw" ftype="bigwig" /> + <param name="title" value="rep 1 test point:0.5" /> + <param name="height_bigwig" value="4" /> + <param name="color" value="black" /> + <conditional name="type_conditional"> + <param name="type_selector" value="point_option" /> + <param name="width_point" value="0.5" /> + + </conditional> + </conditional> + </repeat> + + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="spacer_option" /> + <param name="spacer_height" value="0.5" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="gene_track_option" /> + <param name="track_input_bed" value="dm3_genes.bed.gz" ftype="bed" /> + <param name="title" value="genes" /> + <param name="height_bed" value="5" /> + <param name="fontsize" value="10" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="spacer_option" /> + <param name="spacer_height" value="1" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="gene_track_option" /> + <param name="track_input_bed" value="dm3_genes.bed.gz" ftype="bed" /> + <param name="title" value="max num rows 3" /> + <param name="height_bed" value="3" /> + <param name="fontsize" value="8" /> + <param name="gene_rows" value="3" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="spacer_option" /> + <param name="spacer_height" value="1" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="gene_track_option" /> + <param name="track_input_bed" value="dm3_genes.bed6.gz" ftype="bed" /> + <param name="title" value="bed6 global max row" /> + <param name="height_bed" value="20" /> + <param name="fontsize" value="10" /> + <param name="global_max_row" value="True" /> + </conditional> + </repeat> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="vlines_track_option" /> + <param name="track_input_bed_single" value="domains.bed" ftype="bed" /> + <param name="type" value="vlines" /> + </conditional> + </repeat> + <param name="image_file_format" value="png" /> + <output name="outFileName" file="master_TADs_plot.png" ftype="png" compare="sim_size" delta="35000" /> + </test> + <test> + <param name="region" value="chrX:3000000-3500000"/> + <conditional name="x_axis"> + <param name="x_axis_select" value="yes" /> + </conditional> + <repeat name="tracks"> + <conditional name="track_file_style_conditional"> + <param name="track_file_style_selector" value="bigwig_track_option" /> + <param name="track_input_bigwig" value="bigwig_chrx_2e6_5e6.bw,bigwig_chrx_2e6_5e6.bw" ftype="bigwig" /> + <param name="title" value="rep 1 test line" /> + <param name="height_bigwig" value="4" /> + <param name="color" value="red" /> + <conditional name="type_conditional"> + <param name="type_selector" value="line_option" /> + <param name="width_line" value="1" /> + </conditional> + </conditional> + </repeat> + <param name="image_file_format" value="png" /> + <output name="outFileName" file="bigwig_multiple.png" ftype="png" compare="sim_size" delta="35000" /> + </test> + </tests> + <help><![CDATA[ + +pyGenomeTracks +============== + +**pyGenomeTracks** is a visualization tool which aims to produce high-quality genome browser tracks that are highly customizable. + Currently, it is possible to plot: + + - bigwig + - bed (many options) + - bedgraph + - links (represented as arcs) + - Hi-C matrices + +_________________ + +Usage +----- + +This tool takes various types of tracks as input: + + - **Chromatin states:** display blocks of different colors following a bed file. + - **Gene track / Bed Track:** display genes or bed files. Labels like gene names can be toggled on or off. + - **Bigwig track:** generic bigwig track plotting. + - **Bedgraph track:** generic bedgraph track plotting. + - **Bedgraph matrix track** is used to specifically plot bm files computed by HiCExplorer's ``hicFindTADs`` (TAD seperation scores). + - **Vlines:** vertical lines drawn on top of all tracks following a bed file. It is used as a visual support where regions start / end over all tracks, for example to display TAD boundaries computed by HiCExplorer's ``hicFindTADs``. + - **Spacer:** Add some space between two tracks. + - **Hi-C tracks:** + - **TAD vizualisation:** corrected Hi-C contact matrix to plot a contact heatmap. It is recommended to follow HiCExplorer's ``hicPlotMatrix`` instructions. Boundaries file can used, which is the output of HiCExplorer's ``hicFindTADs`` in bed format. If selected, TADs will be drawn directly on the contact heatmap. + - **TAD score:** display TAD seperation score computed by HiCExplorer's ``hicFindTADs``. + + +For each track, parameters for the color, the width or the font size can be defined. + +_________________ + +Output +------ + +**pyGenomeTracks** output is similar to a genome browser screen-shot that besides the usual genes, and score data (like bigwig or bedgraph files) +also contains Hi-C data. The plot is composed of tracks that need to be specified. +Below is represented the 85 Mb to 110 Mb region from human chromosome 2 visualized using **pyGenomeTracks**. +TADs were computed by HiCExplorer's ``hicFindTADs``. The additional tracks added correspond to: +TAD-separation score (as reported by HiCExplorer's ``hicFindTADs``), chromatin states, +principal component 1 (A/B compartment) computed using HiCExplorer's ``hicPCA``, ChIP-seq coverage for the H3K27ac mark, DNA methylation, and a gene track. +Data are from mouse cardiac myocytes, published by `Nothjunge et al. (2017)`_. + +.. image:: $PATH_TO_IMAGES/pyGenomeTracks.png + :width: 70 % + +_________________ + +| For more information about pyGenomeTracks please consider our documentation on github_ + +.. _github: https://github.com/deeptools/pyGenomeTracks +.. _`Nothjunge et al. (2017)`: https://www.nature.com/articles/s41467-017-01724-9 +]]></help> + <expand macro="citations" /> +</tool>