Mercurial > repos > iuc > qiime_make_otu_heatmap
changeset 3:71b9caeb675b draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a831282140ce160035a4ce984f48cc20198ed0a1
| author | iuc |
|---|---|
| date | Thu, 22 Jun 2017 06:56:26 -0400 |
| parents | bd379bfdaa41 |
| children | 11a2b9162be2 |
| files | generate_test_data.sh macros.xml test-data/assign_taxonomy/mothur_id_to_taxonomy.txt test-data/assign_taxonomy/mothur_repr_set_seqs.fasta test-data/assign_taxonomy/sortmerna_input_seqs.fasta test-data/assign_taxonomy/sortmerna_map.blast test-data/assign_taxonomy/sortmerna_taxonomic_assignation.txt test-data/assign_taxonomy/uclust_taxonomic_assignation.txt |
| diffstat | 8 files changed, 56 insertions(+), 76 deletions(-) [+] |
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--- a/generate_test_data.sh Fri May 19 04:08:04 2017 -0400 +++ b/generate_test_data.sh Thu Jun 22 06:56:26 2017 -0400 @@ -92,9 +92,32 @@ --similarity '0.9' \ --uclust_max_accepts '3' \ -o assign_taxonomy_uclust -cp assign_taxonomy_uclust/uclust_input_seqs_tax_assignments.txt 'test-data/assign_taxonomy/uclust_taxonomic_assignation.txt' +ls assign_taxonomy_uclust +md5sum 'assign_taxonomy_uclust/uclust_input_seqs_tax_assignments.txt' rm -rf assign_taxonomy_uclust +assign_taxonomy.py \ + --input_fasta_fp 'test-data/assign_taxonomy/mothur_repr_set_seqs.fasta' \ + --id_to_taxonomy_fp 'test-data/assign_taxonomy/mothur_id_to_taxonomy.txt' \ + --assignment_method 'mothur' \ + --reference_seqs_fp 'test-data/assign_taxonomy/mothur_ref_seq_set.fna' \ + --confidence '0.5' \ + -o assign_taxonomy_mothur +ls assign_taxonomy_mothur +md5sum 'assign_taxonomy_mothur/mothur_repr_set_seqs_tax_assignments.txt' +rm -rf assign_taxonomy_mothur + +assign_taxonomy.py \ + --input_fasta_fp 'test-data/assign_taxonomy/mothur_repr_set_seqs.fasta' \ + --id_to_taxonomy_fp 'test-data/assign_taxonomy/mothur_id_to_taxonomy.txt' \ + --assignment_method 'mothur' \ + --reference_seqs_fp 'test-data/assign_taxonomy/mothur_ref_seq_set.fna' \ + --blast_e_value '0.001' \ + -o assign_taxonomy_blast +ls assign_taxonomy_blast +md5sum 'assign_taxonomy_blast/mothur_repr_set_seqs_tax_assignments.txt' +rm -rf assign_taxonomy_blast + #assign_taxonomy.py \ # --input_fasta_fp 'test-data/assign_taxonomy/rdp_input_seqs.fasta' \ # --id_to_taxonomy_fp 'test-data/assign_taxonomy/rdp_id_to_taxonomy.txt' \ @@ -116,14 +139,6 @@ # -o assign_taxonomy_rtax #ls assign_taxonomy_rtax -#assign_taxonomy.py \ -# --input_fasta_fp 'test-data/assign_taxonomy/mothur_ref_seq_set.fna' \ -# --id_to_taxonomy_fp 'test-data/assign_taxonomy/mothur_id_to_taxonomy.txt' \ -# --assignment_method 'mothur' \ -# --confidence 0.5 \ -# -o assign_taxonomy_mothur -#ls assign_taxonomy_mothur - assign_taxonomy.py \ --input_fasta_fp 'test-data/assign_taxonomy/mothur_ref_seq_set.fna' \ --assignment_method 'sortmerna' \ @@ -133,8 +148,9 @@ --sortmerna_coverage "0.9" \ --sortmerna_best_N_alignments "5" \ -o assign_taxonomy_sortmerna -cp assign_taxonomy_sortmerna/sortmerna_map.blast 'test-data/assign_taxonomy/sortmerna_map.blast' -cp assign_taxonomy_sortmerna/mothur_ref_seq_set_tax_assignments.txt 'test-data/assign_taxonomy/sortmerna_taxonomic_assignation.txt' +ls assign_taxonomy_sortmerna +md5sum 'assign_taxonomy_sortmerna/mothur_ref_seq_set_tax_assignments.txt' +md5sum 'assign_taxonomy_sortmerna/sortmerna_map.blast' rm -rf assign_taxonomy_sortmerna #beta_diversity @@ -1105,22 +1121,3 @@ cp validate_mapping_file_output/*.log 'test-data/validate_mapping_file/map.tsv.log' cp validate_mapping_file_output/*corrected.txt 'test-data/validate_mapping_file/map.tsv_corrected.txt' rm -rf validate_mapping_file_output - - - - - - - - - - - - - - - - - - -
--- a/macros.xml Fri May 19 04:08:04 2017 -0400 +++ b/macros.xml Thu Jun 22 06:56:26 2017 -0400 @@ -29,6 +29,22 @@ </when> </conditional> </xml> + <xml name="assign_taxonomy_reference_source"> + <conditional name="references"> + <param name="source_selector" type="select" label="Select a reference sequence file from"> + <option value="cached">The local cache</option> + <option value="history">The active history</option> + </param> + <when value="cached"> + <param argument="--reference_seqs_fp" label="Reference sequences either used to generate a blast database (Blast) or used as training sequences for the selected classifier (RDP, Mothur)" type="select"> + <options from_data_table="qiime_rep_set"/> + </param> + </when> + <when value="history"> + <param argument="--reference_seqs_fp" type="data" format="fasta" label="Reference sequences to search against"/> + </when> + </conditional> + </xml> <xml name="pick_otus_similarity"> <param argument="--similarity" type="float" value="0.97" label="Sequence similarity threshold"/> </xml>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/assign_taxonomy/mothur_id_to_taxonomy.txt Thu Jun 22 06:56:26 2017 -0400 @@ -0,0 +1,7 @@ +X67228 Bacteria;Proteobacteria;Alphaproteobacteria;Rhizobiales;Rhizobiaceae;Rhizobium +X73443 Bacteria;Firmicutes;Clostridia;Clostridiales;Clostridiaceae;Clostridium +AB004750 Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Enterobacter +xxxxxx Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Pseudomonadaceae;Pseudomonas +AB004748 Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Enterobacter +AB000278 Bacteria;Proteobacteria;Gammaproteobacteria;Vibrionales;Vibrionaceae;Photobacterium +AB000390 Bacteria;Proteobacteria;Gammaproteobacteria;Vibrionales;Vibrionaceae;Vibrio
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/assign_taxonomy/mothur_repr_set_seqs.fasta Thu Jun 22 06:56:26 2017 -0400 @@ -0,0 +1,4 @@ +>X67228 some description +aacgaacgctggcggcaggcttaacacatgcaagtcgaacgctccgcaaggagagtggcagacgggtgagtaacgcgtgggaatctacccaaccctgcggaatagctctgggaaactggaattaataccgcatacgccctacgggggaaagatttatcggggatggatgagcccgcgttggattagctagttggtggggtaaaggcctaccaaggcgacgatccatagctggtctgagaggatgatcagccacattgggactgagacacggcccaaa +>EF503697 +TAAAATGACTAGCCTGCGAGTCACGCCGTAAGGCGTGGCATACAGGCTCAGTAACACGTAGTCAACATGCCCAAAGGACGTGGATAACCTCGGGAAACTGAGGATAAACCGCGATAGGCCAAGGTTTCTGGAATGAGCTATGGCCGAAATCTATATGGCCTTTGGATTGGACTGCGGCCGATCAGGCTGTTGGTGAGGTAATGGCCCACCAAACCTGTAACCGGTACGGGCTTTGAGAGAAGTAGCCCGGAGATGGGCACTGAGACAAGGGCCCAGGCCCTATGGGGCGCAGCAGGCGCGAAACCTCTGCAATAGGCGAAAGCCTGACAGGGTTACTCTGAGTGATGCCCGCTAAGGGTATCTTTTGGCACCTCTAAAAATGGTGCAGAATAAGGGGTGGGCAAGTCTGGTGTCAGCCGCCGCGGTAATACCAGCACCCCGAGTTGTCGGGACGATTATTGGGCCTAAAGCATCCGTAGCCTGTTCTGCAAGTCCTCCGTTAAATCCACCTGCTCAACGGATGGGCTGCGGAGGATACCGCAGAGCTAGGAGGCGGGAGAGGCAAACGGTACTCAGTGGGTAGGGGTAAAATCCATTGATCTACTGAAGACCACCAGTGGCGAAGGCGGTTTGCCAGAACGCGCTCGACGGTGAGGGATGAAAGCTGGGGGAGCAAACCGGATTAGATACCCGGGGTAGTCCCAGCTGTAAACGGATGCAGACTCGGGTGATGGGGTTGGCTTCCGGCCCAACCCCAATTGCCCCCAGGCGAAGCCCGTTAAGATCTTGCCGCCCTGTCAGATGTCAGGGCCGCCAATACTCGAAACCTTAAAAGGAAATTGGGCGCGGGAAAAGTCACCAAAAGGGGGTTGAAACCCTGCGGGTTATATATTGTAAACC
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/assign_taxonomy/sortmerna_input_seqs.fasta Thu Jun 22 06:56:26 2017 -0400 @@ -0,0 +1,2 @@ +>X67228 +aacgaacgctggcggcaggcttaacacatgcaagtcgaacgctccgcaaggagagtggcagacgggtgagtaacgcgtgggaatctacccaaccctgcggaatagctctgggaaactggaattaataccgcatacgccctacgggggaaagatttatcggggatggatgagcccgcgttggattagctagttggtggggtaaaggcctaccaaggcgacgatccatagctggtctgagaggatgatcagccacattgggactgagacacggcccaaa
--- a/test-data/assign_taxonomy/sortmerna_map.blast Fri May 19 04:08:04 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,28 +0,0 @@ -X67228 152350 98.6 277 4 0 1 277 22 298 5.76e-129 464 277M 100 -X67228 558499 97.1 275 8 0 1 275 2 276 1.05e-122 443 275M2S 99.3 -X67228 553706 97.5 277 7 0 1 277 1 277 4.7e-125 451 277M 100 -X67228 553981 95.7 277 12 0 1 277 2 278 1.55e-118 429 277M 100 -X67228 4423084 98.6 277 4 0 1 277 21 297 5.76e-129 464 277M 100 -X73443 179865 96.3 269 2 8 8 276 2 268 2.31e-114 415 7S3M1I28M1I3M1I7M1D8M1D20M1I26M1I115M1D54M 97.5 -X73443 181718 96 269 3 8 8 276 2 268 4.66e-113 411 7S3M1I28M1I3M1I7M1D8M1D20M1I26M1I115M1D54M 97.5 -X73443 193551 96.3 269 2 8 8 276 2 268 2.31e-114 415 7S3M1I28M1I3M1I7M1D8M1D21M1I26M1I114M1D54M 97.5 -X73443 212341 96.3 269 2 8 8 276 2 268 2.31e-114 415 7S3M1I28M1I3M1I7M1D8M1D21M1I26M1I114M1D54M 97.5 -X73443 175883 96 269 3 8 8 276 2 268 4.66e-113 411 7S3M1I28M1I3M1I7M1D8M1D21M1I26M1I114M1D54M 97.5 -AB004750 3888577 100 339 0 0 1 339 26 364 1.61e-166 588 339M 100 -AB004750 581782 97.6 339 8 0 1 339 27 365 4.36e-156 554 339M 100 -AB004750 1108679 97.9 339 7 0 1 339 26 364 2.16e-157 558 339M 100 -AB004750 1109844 97.9 339 7 0 1 339 26 364 2.16e-157 558 339M 100 -AB004750 4418165 99.7 339 1 0 1 339 28 366 3.25e-165 584 339M 100 -xxxxxx 1102995 97.5 361 8 1 1 361 22 383 2.94e-166 588 174M1D187M 100 -xxxxxx 340031 95.6 361 13 3 1 361 23 386 1.07e-158 562 169M3D192M 100 -xxxxxx 340031 95.6 361 13 3 1 361 23 386 1.07e-158 562 169M3D192M 100 -AB004748 581782 98 396 8 0 1 396 27 422 8.13e-186 653 396M 100 -AB004748 1108679 98.2 396 7 0 1 396 26 421 4.04e-187 657 396M 100 -AB004748 1109844 98.2 396 7 0 1 396 26 421 4.04e-187 657 396M 100 -AB004748 3888577 100 396 0 0 1 396 26 421 3.01e-196 687 396M 100 -AB004748 561327 97.5 396 10 0 1 396 1 396 3.3e-183 644 396M 100 -AB000278 554346 98.6 368 5 0 1 368 6 373 4e-175 617 368M 100 -AB000278 160928 97 368 7 4 1 368 33 400 2.94e-166 588 33M1D5M1I8M1I2M1D318M 100 -AB000390 4433053 98.1 317 6 0 1 317 13 329 3.2e-147 524 317M 100 -AB000390 19456 94.4 317 14 4 1 317 12 328 4.28e-132 474 77M2D4M2I234M 100 -AB000390 4432126 94.4 317 14 4 1 317 13 329 4.28e-132 474 77M2D4M2I234M 100
--- a/test-data/assign_taxonomy/sortmerna_taxonomic_assignation.txt Fri May 19 04:08:04 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,8 +0,0 @@ -#OTU ID taxonomy confidence num hits -AB004750 k__Bacteria; p__Proteobacteria; c__Gammaproteobacteria; o__Enterobacteriales; f__Enterobacteriaceae; g__; s__ 0.60 5 -AB000390 k__Bacteria; p__Proteobacteria; c__Gammaproteobacteria; o__Vibrionales; f__Vibrionaceae 1.00 3 -xxxxxx k__Bacteria; p__Proteobacteria; c__Gammaproteobacteria; o__Alteromonadales; f__Alteromonadaceae; g__Marinobacter; s__ 1.00 3 -X67228 k__Bacteria; p__Proteobacteria; c__Alphaproteobacteria; o__Rhizobiales; f__Rhizobiaceae 0.60 5 -AB000278 k__Bacteria; p__Proteobacteria; c__Gammaproteobacteria; o__Vibrionales; f__Vibrionaceae; g__Photobacterium 1.00 2 -AB004748 k__Bacteria; p__Proteobacteria; c__Gammaproteobacteria; o__Enterobacteriales; f__Enterobacteriaceae; g__; s__ 0.60 5 -X73443 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae 1.00 5
--- a/test-data/assign_taxonomy/uclust_taxonomic_assignation.txt Fri May 19 04:08:04 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,10 +0,0 @@ -11469739 k__Bacteria; p__OP9; c__JS1; o__SB-45; f__; g__; s__ 1.00 3 -11480235 k__Bacteria; p__OD1; c__; o__; f__; g__; s__ 1.00 1 -11460543 k__Bacteria; p__OP9; c__JS1; o__SB-45; f__; g__; s__ 1.00 3 -11460523 k__Bacteria; p__Proteobacteria; c__Deltaproteobacteria; o__Desulfobacterales; f__Desulfobulbaceae; g__; s__ 1.00 3 -11472286 k__Bacteria; p__WS5; c__; o__; f__; g__; s__ 1.00 1 -11458037 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Peptococcaceae; g__Desulfosporosinus; s__meridiei 1.00 3 -11472384 k__Bacteria; p__Proteobacteria; c__Betaproteobacteria; o__Burkholderiales; f__Burkholderiaceae; g__Burkholderia; s__ 0.67 3 -11469752 k__Bacteria; p__TM7; c__TM7-1; o__; f__; g__; s__ 1.00 3 -11480408 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__ 1.00 3 -11468680 k__Bacteria; p__Proteobacteria; c__Betaproteobacteria; o__Burkholderiales; f__Burkholderiaceae; g__Burkholderia; s__ 1.00 3