Mercurial > repos > iuc > qiime_pick_open_reference_otus
diff pick_open_reference_otus.xml @ 3:41482ca21d31 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit 3b54163c4f7daff76fcc589c4a9057bb03904380
author | iuc |
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date | Sat, 05 Aug 2017 07:24:40 -0400 |
parents | f668a753bcfc |
children | bf3348cc35d5 |
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--- a/pick_open_reference_otus.xml Mon Jul 10 16:43:22 2017 -0400 +++ b/pick_open_reference_otus.xml Sat Aug 05 07:24:40 2017 -0400 @@ -1,64 +1,63 @@ <tool id="qiime_pick_open_reference_otus" name="Perform open-reference OTU picking" version="@WRAPPER_VERSION@.0"> - <description></description> + <description>(pick_open_reference_otus)</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"/> <version_command>pick_open_reference_otus.py --version</version_command> <command detect_errors="aggressive"><![CDATA[ - pick_open_reference_otus.py - --input_fps '$input_fps' - -o otus - --otu_picking_method '$otu_picking_method' - #if str( $ref_sequences.selector ) == 'history' - #set $ref = $ref_sequences.reference_fp - #else: - #set $ref = $ref_sequences.reference_fp.fields.path - #end if - --reference_fp '$ref' - #if str( $prefilter.use_prefilter ) == 'yes' - #if str( $prefilter.prefilter_ref_seq.selector ) == 'history' - #set $ref = $prefilter.prefilter_ref_seq.prefilter_refseqs_fp - #else: - #set $ref = $prefilter.prefilter_ref_seq.prefilter_refseqs_fp.fields.path - #end if - --prefilter_refseqs_fp '$ref' - #end if - --new_ref_set_id '$new_ref_set_id' - $parallel - -O "\${GALAXY_SLOTS:-4}" - --percent_subsample '$percent_subsample' - --prefilter_percent_id '$prefilter_percent_id' - #if str($step1_otu_map_fp) != 'None': - --step1_otu_map_fp '$step1_otu_map_fp' - #end if - #if str($step1_failures_fasta_fp) != 'None': - --step1_failures_fasta_fp='$step1_failures_fasta_fp' - #end if - --minimum_failure_threshold '$minimum_failure_threshold' - $suppress_step4 - --min_otu_size '$min_otu_size' - $suppress_taxonomy_assignment - $suppress_align_and_tree +pick_open_reference_otus.py + --input_fps '$input_fps' + -o otus + --otu_picking_method '$otu_picking_method' + #if str( $ref_sequences.selector ) == 'history' + #set $ref = $ref_sequences.reference_fp + #else: + #set $ref = $ref_sequences.reference_fp.fields.path + #end if + --reference_fp '$ref' + #if str( $prefilter.use_prefilter ) == 'yes' + #if str( $prefilter.prefilter_ref_seq.selector ) == 'history' + #set $ref = $prefilter.prefilter_ref_seq.prefilter_refseqs_fp + #else: + #set $ref = $prefilter.prefilter_ref_seq.prefilter_refseqs_fp.fields.path + #end if + --prefilter_refseqs_fp '$ref' + #end if + --new_ref_set_id '$new_ref_set_id' + $parallel + -O "\${GALAXY_SLOTS:-4}" + --percent_subsample '$percent_subsample' + --prefilter_percent_id '$prefilter_percent_id' + #if str($step1_otu_map_fp) != 'None': + --step1_otu_map_fp '$step1_otu_map_fp' + #end if + #if str($step1_failures_fasta_fp) != 'None': + --step1_failures_fasta_fp='$step1_failures_fasta_fp' + #end if + --minimum_failure_threshold '$minimum_failure_threshold' + $suppress_step4 + --min_otu_size '$min_otu_size' + $suppress_taxonomy_assignment + $suppress_align_and_tree - #if str( $suppress_align_and_tree ) == "" - #if str( $suppress_taxonomy_assignment ) == "" - && mv otus/otu_table_mc*_w_tax.biom otus/otu_table_w_tax.biom - && mv otus/otu_table_mc*_w_tax_no_pynast_failures.biom otus/tu_table_w_tax_no_pynast_failures - #else - && mv otus/otu_table_mc*_no_pynast_failures.biom otus/otu_table_no_pynast_failures.biom - #end if - #end if - +#if str( $suppress_align_and_tree ) == "" + #if str( $suppress_taxonomy_assignment ) == "" + && mv otus/otu_table_mc*_w_tax.biom otus/otu_table_w_tax.biom + && mv otus/otu_table_mc*_w_tax_no_pynast_failures.biom otus/tu_table_w_tax_no_pynast_failures + #else + && mv otus/otu_table_mc*_no_pynast_failures.biom otus/otu_table_no_pynast_failures.biom + #end if +#end if ]]></command> <inputs> - <param argument="--input_fps" type="data" format="fasta,fastq" multiple="True" label="Input sequence files"/> + <param argument="--input_fps" type="data" format="fasta,fastq" multiple="true" label="Input sequence files"/> <param argument="--otu_picking_method" type="select" label="The OTU picking method to use for reference and de novo steps"> - <option value="uclust" selected="True">uclust</option> + <option value="uclust" selected="true">uclust</option> </param> <conditional name="ref_sequences"> - <param name="selector" type="select" label="Reference sequences to query"> - <option value="cached" selected="True">Public databases</option> + <param name="selector" type="select" label="Reference sequences to query"> + <option value="cached" selected="true">Public databases</option> <option value="history">Databases from your history</option> </param> <when value="cached"> @@ -73,12 +72,12 @@ <conditional name="prefilter"> <param name="use_prefilter" type="select" label="Use different reference sequences for the prefilter?"> <option value="true">True</option> - <option value="false" selected="True">False</option> + <option value="false" selected="true">False</option> </param> <when value="true"> <conditional name="prefilter_ref_seq"> <param name="selector" type="select" label="Reference sequences to query"> - <option value="cached" selected="True">Public databases</option> + <option value="cached" selected="true">Public databases</option> <option value="history">Databases from your history</option> </param> <when value="cached"> @@ -94,16 +93,16 @@ <when value="false"/> </conditional> <param argument="--new_ref_set_id" type="text" value="New" label="Unique identifier for OTUs that get created in this ref set" help="This is useful to support combining of reference sets"/> - <param argument="--parallel" type="boolean" truevalue="--parallel" falsevalue="" checked="False" label="Run in parallel where available?"/> + <param argument="--parallel" type="boolean" truevalue="--parallel" falsevalue="" checked="false" label="Run in parallel where available?"/> <param argument="--percent_subsample" type="float" value="0.001" min="0" max="1" label="Percent of failure sequences to include in the subsample to cluster de novo" help="Large numbers should give more comprehensive results but will be slower"/> <param argument="--prefilter_percent_id" type="float" value="0.0" min="0" max="1" label="Percent of sequence pre-cluster against the reference" help="Any reads which fail to hit are discarded (a quality filter)"/> - <param argument="--step1_otu_map_fp" type="data" format="txt" optional="True" label="Reference OTU picking OTU map to avoid rebuilding if one has already been built (optional)" help="This must be an OTU map generated by this workflow, not (for example) by pick_closed_reference_otus"/> - <param argument="--step1_failures_fasta_fp" type="data" format="fasta" optional="True" label="Reference OTU picking failures fasta filepath, to avoid rebuilding if one has already been built (optional)" help="This must be a failures file generated by this workflow, not (for example) by pick_closed_reference_otus"/> + <param argument="--step1_otu_map_fp" type="data" format="txt" optional="true" label="Reference OTU picking OTU map to avoid rebuilding if one has already been built (optional)" help="This must be an OTU map generated by this workflow, not (for example) by pick_closed_reference_otus"/> + <param argument="--step1_failures_fasta_fp" type="data" format="fasta" optional="true" label="Reference OTU picking failures fasta filepath, to avoid rebuilding if one has already been built (optional)" help="This must be a failures file generated by this workflow, not (for example) by pick_closed_reference_otus"/> <param argument="--minimum_failure_threshold" type="integer" value="100000" label="Minimum number of sequences that must fail to hit the reference for subsampling to be performed" help="If fewer than this number of sequences fail to hit the reference, the de novo clustering step will run serially rather than invoking the subsampled open reference approach to improve performance"/> - <param argument="--suppress_step4" type="boolean" truevalue="--suppress_step4" falsevalue="" checked="False" label="Suppress the final de novo OTU picking step?" help="It may be necessary for extremely large data sets"/> - <param argument="--min_otu_size" type="integer" value="2" label="Minimum otu size to retain the OTU" help=""/> - <param argument="--suppress_taxonomy_assignment" type="boolean" truevalue="--suppress_taxonomy_assignment" falsevalue="" checked="False" label="Skip the taxonomy assignment step?" help="It results in an OTU table without taxonomy"/> - <param argument="--suppress_align_and_tree" type="boolean" truevalue="--suppress_align_and_tree" falsevalue="" checked="False" label="Skip the sequence alignment and tree-building steps?" help=""/> + <param argument="--suppress_step4" type="boolean" truevalue="--suppress_step4" falsevalue="" checked="false" label="Suppress the final de novo OTU picking step?" help="It may be necessary for extremely large data sets"/> + <param argument="--min_otu_size" type="integer" value="2" label="Minimum otu size to retain the OTU"/> + <param argument="--suppress_taxonomy_assignment" type="boolean" truevalue="--suppress_taxonomy_assignment" falsevalue="" checked="false" label="Skip the taxonomy assignment step?" help="It results in an OTU table without taxonomy"/> + <param argument="--suppress_align_and_tree" type="boolean" truevalue="--suppress_align_and_tree" falsevalue="" checked="false" label="Skip the sequence alignment and tree-building steps?"/> </inputs> <outputs> <data name="final_otu_map" format="txt" from_work_dir="otus/final_otu_map.txt" label="${tool.name} on ${on_string}: Final OTU map"/>