Mercurial > repos > iuc > qiime_pick_otus
annotate generate_test_data.sh.orig @ 1:f25f5c21d2e4 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
author | iuc |
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date | Fri, 19 May 2017 04:06:41 -0400 |
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f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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1 #!/usr/bin/env bash |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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2 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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3 # validate_mapping_file |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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4 validate_mapping_file.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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5 -m 'test-data/validate_mapping_file/map.tsv' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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6 -o validate_mapping_file_output \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
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7 -c '_' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
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8 cp validate_mapping_file_output/*.html 'test-data/validate_mapping_file/map.tsv.html' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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9 cp validate_mapping_file_output/*.log 'test-data/validate_mapping_file/map.tsv.log' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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10 cp validate_mapping_file_output/*corrected.txt 'test-data/validate_mapping_file/map.tsv_corrected.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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11 rm -rf validate_mapping_file_output |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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12 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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13 # split_libraries_fastq |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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14 split_libraries_fastq.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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15 --sequence_read_fps 'test-data/split_libraries_fastq/forward_reads.fastq' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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16 -o split_libraries \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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17 --mapping_fps 'test-data/map.tsv' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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18 --barcode_read_fps 'test-data/split_libraries_fastq/barcodes.fastq' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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19 --store_qual_scores \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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20 --store_demultiplexed_fastq \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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21 --max_bad_run_length 3 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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22 --min_per_read_length_fraction 0.75 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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23 --sequence_max_n 0 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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24 --start_seq_id 0 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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25 --barcode_type 'golay_12' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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26 --max_barcode_errors 1.5 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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27 cp split_libraries/histograms.txt 'test-data/split_libraries_fastq/histograms.tabular' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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28 cp split_libraries/seqs.fna 'test-data/split_libraries_fastq/sequences.fasta' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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29 cp split_libraries/seqs.qual 'test-data/split_libraries_fastq/sequence_qualities.qual' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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30 cp split_libraries/seqs.fastq 'test-data/split_libraries_fastq/demultiplexed_sequences.fastq' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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31 rm -rf split_libraries |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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32 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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33 # split_libraries |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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changeset
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34 split_libraries.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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35 --map 'test-data/split_libraries/mapping_file.txt' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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36 -o split_libraries \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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37 --fasta 'test-data/split_libraries/reads_1.fna,test-data/split_libraries/reads_2.fna' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
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38 --qual 'test-data/split_libraries/reads_1.qual,test-data/split_libraries/reads_2.qual' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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39 --min_qual_score 25 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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40 --qual_score_window 0 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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41 --record_qual_scores \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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42 --min_seq_length 200 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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43 --max_seq_length 1000 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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44 --max_ambig 6 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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45 --max_homopolymer 6 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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46 --max_primer_mismatch 0 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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47 --barcode_type 'golay_12' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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48 --max_barcode_errors 1.5 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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49 --start_numbering_at 1 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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50 cp split_libraries/seqs.fna 'test-data/split_libraries/seqs.fna' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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51 cp split_libraries/split_library_log.txt 'test-data/split_libraries/split_library_log' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
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52 cp split_libraries/histograms.txt 'test-data/split_libraries/histograms.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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53 cp split_libraries/seqs_filtered.qual 'test-data/split_libraries/seqs_filtered.qual' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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54 rm -rf split_libraries |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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55 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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56 # pick_open_reference_otus |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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57 pick_open_reference_otus.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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58 --input_fps 'test-data/pick_open_reference_otus/sequences.fasta' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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59 -o pick_open_reference_otus_1 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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60 --reference_fp 'test-data/gg_13_8_79_otus.fasta' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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61 --otu_picking_method 'uclust' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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62 --new_ref_set_id 'New' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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63 --parallel \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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64 --percent_subsample '0.001' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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65 --prefilter_percent_id '0.0' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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66 --minimum_failure_threshold '100000' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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67 --min_otu_size '2' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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68 cp pick_open_reference_otus_1/final_otu_map.txt 'test-data/pick_open_reference_otus/1_final_otu_map.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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69 cp pick_open_reference_otus_1/final_otu_map_mc*.txt 'test-data/pick_open_reference_otus/1_final_otu_map_mc.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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70 cp pick_open_reference_otus_1/rep_set.tre 'test-data/pick_open_reference_otus/1_rep_set_tree.tre' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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71 rm -rf pick_open_reference_otus_1 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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72 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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73 pick_open_reference_otus.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
diff
changeset
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74 --input_fps 'test-data/pick_open_reference_otus/sequences.fasta' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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changeset
|
75 -o pick_open_reference_otus_2 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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76 --reference_fp 'test-data/gg_13_8_79_otus.fasta' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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changeset
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77 --otu_picking_method 'uclust' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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78 --new_ref_set_id 'New' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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79 --parallel \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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80 --percent_subsample '0.001' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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81 --prefilter_percent_id '0.0' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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82 --minimum_failure_threshold '100000' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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83 --min_otu_size '3' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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84 --suppress_taxonomy_assignment \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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85 --suppress_align_and_tree |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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86 cp pick_open_reference_otus_2/final_otu_map.txt 'test-data/pick_open_reference_otus/2_final_otu_map.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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87 cp pick_open_reference_otus_2/final_otu_map_mc*.txt 'test-data/pick_open_reference_otus/2_final_otu_map_mc.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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88 rm -rf pick_open_reference_otus_2 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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89 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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90 pick_open_reference_otus.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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91 --input_fps 'test-data/pick_open_reference_otus/sequences.fasta' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
parents:
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92 -o pick_open_reference_otus_3 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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parents:
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93 --reference_fp 'test-data/gg_13_8_79_otus.fasta' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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parents:
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94 --otu_picking_method 'uclust' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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95 --new_ref_set_id 'New' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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96 --parallel \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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97 --percent_subsample '0.001' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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98 --prefilter_percent_id '0.0' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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99 --minimum_failure_threshold '100000' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
iuc
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100 --min_otu_size '10' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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101 --suppress_taxonomy_assignment |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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102 cp pick_open_reference_otus_3/final_otu_map.txt 'test-data/pick_open_reference_otus/3_final_otu_map.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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103 cp pick_open_reference_otus_3/final_otu_map_mc*.txt 'test-data/pick_open_reference_otus/3_final_otu_map_mc.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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104 cp pick_open_reference_otus_3/rep_set.tre 'test-data/pick_open_reference_otus/3_rep_set_tree.tre' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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105 rm -rf pick_open_reference_otus_3 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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106 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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107 # core_diversity_analyses |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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108 # Data are from test data in https://github.com/biocore/qiime |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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109 core_diversity_analyses.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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110 --input_biom_fp 'test-data/core_diversity_analyses/otu_table.biom' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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111 -o core_diversity_analyses_1 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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112 --mapping_fp 'test-data/core_diversity_analyses/map.txt' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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113 --sampling_depth 22 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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114 --tree_fp 'test-data/core_diversity_analyses/rep_set.tre' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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115 cp core_diversity_analyses_1/bdiv_even22/unweighted_unifrac_pc.txt 'test-data/core_diversity_analyses/unweighted_unifrac_pc.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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116 rm -rf core_diversity_analyses_1 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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117 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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118 core_diversity_analyses.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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119 --input_biom_fp 'test-data/core_diversity_analyses/otu_table.biom' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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120 -o core_diversity_analyses_2 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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121 --mapping_fp 'test-data/core_diversity_analyses/map.txt' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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122 --sampling_depth 22 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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123 --nonphylogenetic_diversity \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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124 --suppress_taxa_summary \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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125 --suppress_beta_diversity \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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126 --suppress_alpha_diversity \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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127 --suppress_group_significance |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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128 rm -rf core_diversity_analyses_2 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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129 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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130 # summarize_taxa |
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131 cp 'test-data/core_diversity_analyses/otu_table.biom' 'test-data/summarize_taxa/otu_table.biom' |
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132 cp 'test-data/core_diversity_analyses/map.txt' 'test-data/summarize_taxa/map.txt' |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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133 |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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134 summarize_taxa.py \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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135 -i 'test-data/summarize_taxa/otu_table.biom' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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136 -o summarize_taxa_1 \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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137 -L '2,3,4,5,6' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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138 -m 'test-data/summarize_taxa/map.txt' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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139 --md_identifier "taxonomy" \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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140 --delimiter ";" |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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141 cp summarize_taxa_1/*_L2.txt "test-data/summarize_taxa/1_L2.txt" |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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142 cp summarize_taxa_1/*_L3.txt "test-data/summarize_taxa/1_L3.txt" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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143 cp summarize_taxa_1/*_L4.txt "test-data/summarize_taxa/1_L4.txt" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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144 cp summarize_taxa_1/*_L5.txt "test-data/summarize_taxa/1_L5.txt" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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145 cp summarize_taxa_1/*_L6.txt "test-data/summarize_taxa/1_L6.txt" |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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146 rm -rf summarize_taxa_1 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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147 |
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148 summarize_taxa.py \ |
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149 -i 'test-data/summarize_taxa/otu_table.biom' \ |
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150 -o summarize_taxa_2 \ |
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151 -L '3,6' \ |
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152 --md_identifier "taxonomy" \ |
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153 --delimiter ";" |
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154 cp summarize_taxa_2/*_L3.txt "test-data/summarize_taxa/2_L3.txt" |
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155 cp summarize_taxa_2/*_L6.txt "test-data/summarize_taxa/2_L6.txt" |
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156 rm -rf summarize_taxa_2 |
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157 |
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158 # make_emperor |
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159 cp 'test-data/core_diversity_analyses/unweighted_unifrac_pc.txt' 'test-data/make_emperor/unweighted_unifrac_pc.txt' |
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160 cp 'test-data/core_diversity_analyses/map.txt' 'test-data/make_emperor/map.txt' |
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161 cp 'test-data/summarize_taxa/2_L3.txt' 'test-data/make_emperor/2_L3.txt' |
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162 |
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163 make_emperor.py \ |
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164 --input_coords 'test-data/make_emperor/unweighted_unifrac_pc.txt' \ |
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165 -o make_emperor_1 \ |
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166 --map_fp 'test-data/make_emperor/map.txt' \ |
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167 --number_of_axes '10' \ |
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168 --add_unique_columns \ |
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169 --number_of_segments 8 |
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170 rm -rf make_emperor_1 |
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171 |
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172 make_emperor.py \ |
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173 --input_coords 'test-data/make_emperor/unweighted_unifrac_pc.txt' \ |
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174 -o make_emperor_2 \ |
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175 --map_fp 'test-data/make_emperor/map.txt' \ |
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176 --number_of_axes '10' \ |
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177 --add_unique_columns \ |
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178 --number_of_segments 8 \ |
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179 --taxa_fp 'test-data/make_emperor/2_L3.txt' \ |
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180 --n_taxa_to_keep 10 |
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181 rm -rf make_emperor_2 |
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182 |
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183 #alpha_rarefaction |
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184 alpha_rarefaction.py \ |
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185 --otu_table_fp "test-data/alpha_rarefaction/otu_table.biom" \ |
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186 --mapping_fp "test-data/alpha_rarefaction/mapping_file.txt" \ |
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187 -o alpha_rarefaction \ |
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188 --num_steps '2' \ |
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189 --tree_fp "test-data/alpha_rarefaction/rep_set.tre" \ |
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190 --min_rare_depth '10' \ |
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191 --max_rare_depth '50' \ |
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192 --retain_intermediate_files |
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193 rm -rf alpha_rarefaction |
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194 |
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195 ##beta_diversity |
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196 beta_diversity.py \ |
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197 --input_path 'test-data/beta_diversity/otu_table.biom' \ |
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198 -o beta_diversity_1 \ |
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199 --metrics 'unweighted_unifrac,weighted_unifrac' \ |
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200 --tree_path 'test-data/beta_diversity/rep_set.tre' |
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201 md5 'beta_diversity_1/unweighted_unifrac_otu_table.txt' |
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202 md5 'beta_diversity_1/weighted_unifrac_otu_table.txt' |
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203 rm -rf beta_diversity_1 |
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204 |
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205 beta_diversity.py \ |
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206 --input_path 'test-data/beta_diversity/otu_table.biom' \ |
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207 -o beta_diversity_2 \ |
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208 --metrics 'abund_jaccard,binary_chisq,binary_chord,binary_euclidean,binary_hamming,binary_jaccard,binary_lennon,binary_ochiai,binary_pearson,binary_sorensen_dice,bray_curtis,canberra,chisq,chord,euclidean,gower,hellinger,kulczynski,manhattan,morisita_horn,pearson,soergel,spearman_approx,specprof,unifrac_g,unifrac_g_full_tree,unweighted_unifrac,unweighted_unifrac_full_tree,weighted_normalized_unifrac,weighted_unifrac' \ |
f25f5c21d2e4
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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209 --tree_path 'test-data/beta_diversity/rep_set.tre' |
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210 md5 'beta_diversity_2/canberra_otu_table.txt' |
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211 md5 'beta_diversity_2/pearson_otu_table.txt' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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212 rm -rf beta_diversity_2 |
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213 |
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214 # jackknifed_beta_diversity |
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215 jackknifed_beta_diversity.py \ |
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216 --otu_table_fp 'test-data/jackknifed_beta_diversity/otu_table.biom' \ |
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217 --mapping_fp 'test-data/jackknifed_beta_diversity/map.txt' \ |
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218 -o jackknifed_beta_diversity \ |
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219 --seqs_per_sample '10' \ |
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220 --tree_fp 'test-data/jackknifed_beta_diversity/rep_set.tre' \ |
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221 --master_tree 'consensus' \ |
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222 --parallel |
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223 rm -rf jackknifed_beta_diversity |
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224 |
f25f5c21d2e4
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225 #beta_diversity_through_plots |
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226 beta_diversity_through_plots.py \ |
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227 --otu_table_fp 'test-data/beta_diversity_through_plots/otu_table.biom' \ |
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228 --mapping_fp 'test-data/beta_diversity_through_plots/map.txt' \ |
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229 --output_dir beta_diversity_through_plots \ |
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230 --tree_fp 'test-data/beta_diversity_through_plots/rep_set.tre' \ |
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231 --parallel |
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232 cp beta_diversity_through_plots/unweighted_unifrac_dm.txt 'test-data/beta_diversity_through_plots/' |
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233 cp beta_diversity_through_plots/unweighted_unifrac_pc.txt 'test-data/beta_diversity_through_plots/' |
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234 cp beta_diversity_through_plots/weighted_unifrac_dm.txt 'test-data/beta_diversity_through_plots/' |
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235 cp beta_diversity_through_plots/weighted_unifrac_pc.txt 'test-data/beta_diversity_through_plots/' |
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236 rm -rf beta_diversity_through_plots |
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237 |
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238 # assign_taxonomy |
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239 assign_taxonomy.py \ |
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240 --input_fasta_fp 'test-data/assign_taxonomy/uclust_input_seqs.fasta' \ |
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241 --assignment_method 'uclust' \ |
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242 --min_consensus_fraction '0.51' \ |
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243 --similarity '0.9' \ |
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244 --uclust_max_accepts '3' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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245 -o assign_taxonomy_uclust |
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246 cp assign_taxonomy_uclust/uclust_input_seqs_tax_assignments.txt 'test-data/assign_taxonomy/uclust_taxonomic_assignation.txt' |
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247 rm -rf assign_taxonomy_uclust |
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248 |
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249 #assign_taxonomy.py \ |
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250 # --input_fasta_fp 'test-data/assign_taxonomy/rdp_input_seqs.fasta' \ |
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251 # --id_to_taxonomy_fp 'test-data/assign_taxonomy/rdp_id_to_taxonomy.txt' \ |
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252 # --assignment_method 'rdp' \ |
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253 # --confidence '3' \ |
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254 # -o assign_taxonomy_rdp |
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255 |
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256 #assign_taxonomy.py \ |
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257 # --input_fasta_fp 'test-data/assign_taxonomy/rtax_ref_seq_set.fna' \ |
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258 # --id_to_taxonomy_fp 'test-data/assign_taxonomy/rtax_id_to_taxonomy.txt' \ |
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259 # --assignment_method 'rtax' \ |
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260 # --read_1_seqs_fp 'test-data/assign_taxonomy/read_1.seqs.fna' \ |
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261 # --read_2_seqs_fp 'test-data/assign_taxonomy/read_2.seqs.fna' \ |
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262 # --single_ok \ |
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263 # --no_single_ok_generic \ |
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264 # --read_id_regex "\S+\s+(\S+)" \ |
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265 # --amplicon_id_regex "(\S+)\s+(\S+?)\/" \ |
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266 # --header_id_regex "\S+\s+(\S+?)\/" \ |
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267 # -o assign_taxonomy_rtax |
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268 #ls assign_taxonomy_rtax |
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269 |
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270 #assign_taxonomy.py \ |
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271 # --input_fasta_fp 'test-data/assign_taxonomy/mothur_ref_seq_set.fna' \ |
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272 # --id_to_taxonomy_fp 'test-data/assign_taxonomy/mothur_id_to_taxonomy.txt' \ |
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273 # --assignment_method 'mothur' \ |
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274 # --confidence 0.5 \ |
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275 # -o assign_taxonomy_mothur |
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276 #ls assign_taxonomy_mothur |
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277 |
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278 assign_taxonomy.py \ |
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279 --input_fasta_fp 'test-data/assign_taxonomy/mothur_ref_seq_set.fna' \ |
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280 --assignment_method 'sortmerna' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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281 --min_consensus_fraction "0.51" \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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282 --similarity "0.9" \ |
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283 --sortmerna_e_value "1.0" \ |
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284 --sortmerna_coverage "0.9" \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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285 --sortmerna_best_N_alignments "5" \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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286 -o assign_taxonomy_sortmerna |
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287 cp assign_taxonomy_sortmerna/sortmerna_map.blast 'test-data/assign_taxonomy/sortmerna_map.blast' |
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288 cp assign_taxonomy_sortmerna/mothur_ref_seq_set_tax_assignments.txt 'test-data/assign_taxonomy/sortmerna_taxonomic_assignation.txt' |
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289 rm -rf assign_taxonomy_sortmerna |
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290 <<<<<<< HEAD |
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291 ======= |
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292 |
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293 # filter_otus_from_otu_table |
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294 filter_otus_from_otu_table.py \ |
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295 --input_fp 'test-data/filter_otus_from_otu_table/otu_table.biom' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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296 --min_count '2' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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297 --max_count '1000' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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298 --min_samples '5' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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299 --max_samples '350' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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300 --output_fp 'test-data/filter_otus_from_otu_table/filtered_otu_table.biom' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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301 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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302 filter_otus_from_otu_table.py \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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303 --input_fp 'test-data/filter_otus_from_otu_table/otu_table.biom' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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304 --otu_ids_to_exclude_fp 'test-data/filter_otus_from_otu_table/chimeric_otus.txt' \ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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305 --output_fp 'test-data/filter_otus_from_otu_table/chimera_filtered_otu_table.biom' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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306 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit a9d1e0debcd357d8080a1c6c5f1d206dd45a7a4d
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307 filter_otus_from_otu_table.py \ |
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308 --input_fp 'test-data/filter_otus_from_otu_table/otu_table.biom' \ |
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309 --otu_ids_to_exclude_fp 'test-data/filter_otus_from_otu_table/chimeric_otus.txt' \ |
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310 --negate_ids_to_exclude \ |
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311 --output_fp 'test-data/filter_otus_from_otu_table/chimera_picked_otu_table.biom' |
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312 >>>>>>> Update of the Qiime_core generate_test_data script |