# HG changeset patch # User iuc # Date 1501932437 14400 # Node ID 8218f848c2b989fd4c95f6bd5a4b3a6cc0e002da # Parent 10214bba6195b581190df31355d50d9fa7a49145 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qiime/ commit 3b54163c4f7daff76fcc589c4a9057bb03904380 diff -r 10214bba6195 -r 8218f848c2b9 generate_test_data.sh --- a/generate_test_data.sh Mon Jul 10 16:42:21 2017 -0400 +++ b/generate_test_data.sh Sat Aug 05 07:27:17 2017 -0400 @@ -228,6 +228,54 @@ --suppress_group_significance rm -rf core_diversity_analyses_2 +# extract_barcodes +extract_barcodes.py \ + --fastq1 'test-data/extract_barcodes/inseqs.fastq' \ + --input_type 'barcode_single_end' \ + -o extract_barcodes_1 \ + --bc1_len '6' \ + --rev_comp_bc1 +rm -rf extract_barcodes_1 + +extract_barcodes.py \ + --fastq1 'test-data/extract_barcodes/inseqs_R1.fastq' \ + --input_type 'barcode_paired_end' \ + --fastq2 'test-data/extract_barcodes/inseqs_R2.fastq' \ + -o extract_barcodes_2 \ + --bc1_len '6' \ + --bc2_len '6' +rm -rf extract_barcodes_2 + +extract_barcodes.py \ + --fastq1 'test-data/extract_barcodes/inseqs_R1.fastq' \ + --input_type 'barcode_paired_end' \ + --fastq2 'test-data/extract_barcodes/inseqs_R2.fastq' \ + -o extract_barcodes_3 \ + --bc1_len '6' \ + --bc2_len '6' \ + --mapping_fp 'test-data/extract_barcodes/mapping_data.txt' \ + --attempt_read_reorientation \ + --disable_header_match +rm -rf extract_barcodes_3 + +extract_barcodes.py \ + --fastq1 'test-data/extract_barcodes/inseqs_R1.fastq' \ + --input_type 'barcode_paired_stitched' \ + -o extract_barcodes_4 \ + --bc1_len '6' \ + --bc2_len '8' \ + --rev_comp_bc1 \ + --rev_comp_bc2 +rm -rf extract_barcodes_4 + +extract_barcodes.py \ + --fastq1 'test-data/extract_barcodes/inseqs_R1.fastq' \ + --input_type 'barcode_in_label' \ + --char_delineator '#' \ + -o extract_barcodes_5 \ + --bc1_len '6' +rm -rf extract_barcodes_5 + # filter_alignment filter_alignment.py \ --input_fasta_file 'test-data/filter_alignment/alignment.fasta' \ @@ -943,23 +991,79 @@ # split_libraries_fastq split_libraries_fastq.py \ - --sequence_read_fps 'test-data/split_libraries_fastq/forward_reads.fastq' \ - -o split_libraries \ - --mapping_fps 'test-data/map.tsv' \ - --barcode_read_fps 'test-data/split_libraries_fastq/barcodes.fastq' \ + --sequence_read_fps 'test-data/split_libraries_fastq/lane1_read1.fastq.gz' \ + -o split_libraries_1 \ + --mapping_fps 'test-data/split_libraries_fastq/map.txt' \ + --barcode_read_fps 'test-data/split_libraries_fastq/lane1_barcode.fastq.gz' \ + --max_bad_run_length 3 \ + --min_per_read_length_fraction 0.75 \ + --sequence_max_n 0 \ + --start_seq_id 0 \ + --rev_comp_mapping_barcodes \ + --phred_quality_threshold 19 \ + --barcode_type 'golay_12' \ + --max_barcode_errors 1.5 +rm -rf split_libraries_1 + +split_libraries_fastq.py \ + --sequence_read_fps 'test-data/split_libraries_fastq/lane1_read1.fastq.gz' \ + -o split_libraries_2 \ + --mapping_fps 'test-data/split_libraries_fastq/map.txt' \ + --barcode_read_fps 'test-data/split_libraries_fastq/lane1_barcode.fastq.gz' \ --store_qual_scores \ --store_demultiplexed_fastq \ --max_bad_run_length 3 \ --min_per_read_length_fraction 0.75 \ --sequence_max_n 0 \ --start_seq_id 0 \ + --rev_comp_mapping_barcodes \ + --phred_quality_threshold 19 \ + --barcode_type 'golay_12' \ + --max_barcode_errors 1.5 +rm -rf split_libraries_2 + +split_libraries_fastq.py \ + --sequence_read_fps 'test-data/split_libraries_fastq/lane1_read1.fastq.gz,test-data/split_libraries_fastq/lane2_read1.fastq.gz' \ + -o split_libraries_3 \ + --mapping_fps 'test-data/split_libraries_fastq/map.txt,test-data/split_libraries_fastq/map.txt' \ + --barcode_read_fps 'test-data/split_libraries_fastq/lane1_barcode.fastq.gz,test-data/split_libraries_fastq/lane2_barcode.fastq.gz' \ + --max_bad_run_length 3 \ + --min_per_read_length_fraction 0.75 \ + --sequence_max_n 0 \ + --start_seq_id 0 \ + --rev_comp_mapping_barcodes \ + --phred_quality_threshold 19 \ --barcode_type 'golay_12' \ --max_barcode_errors 1.5 -cp split_libraries/histograms.txt 'test-data/split_libraries_fastq/histograms.tabular' -cp split_libraries/seqs.fna 'test-data/split_libraries_fastq/sequences.fasta' -cp split_libraries/seqs.qual 'test-data/split_libraries_fastq/sequence_qualities.qual' -cp split_libraries/seqs.fastq 'test-data/split_libraries_fastq/demultiplexed_sequences.fastq' -rm -rf split_libraries +rm -rf split_libraries_3 + +split_libraries_fastq.py \ + --sequence_read_fps 'test-data/split_libraries_fastq/lane1_read1.fastq.gz' \ + -o split_libraries_4 \ + --sample_ids 'my.sample.1' \ + --max_bad_run_length 3 \ + --min_per_read_length_fraction 0.75 \ + --sequence_max_n 0 \ + --start_seq_id 0 \ + --rev_comp_mapping_barcodes \ + --phred_quality_threshold 19 \ + --barcode_type 'not-barcoded' \ + --max_barcode_errors 1.5 +rm -rf split_libraries_4 + +split_libraries_fastq.py \ + --sequence_read_fps 'test-data/split_libraries_fastq/lane1_read1.fastq.gz,test-data/split_libraries_fastq/lane2_read1.fastq.gz' \ + -o split_libraries_5 \ + --sample_ids 'my.sample.1,my.sample.2' \ + --max_bad_run_length 3 \ + --min_per_read_length_fraction 0.75 \ + --sequence_max_n 0 \ + --start_seq_id 0 \ + --rev_comp_mapping_barcodes \ + --phred_quality_threshold 19 \ + --barcode_type 'not-barcoded' \ + --max_barcode_errors 1.5 +rm -rf split_libraries_5 # summarize_taxa cp 'test-data/core_diversity_analyses/otu_table.biom' 'test-data/summarize_taxa/otu_table.biom' diff -r 10214bba6195 -r 8218f848c2b9 macros.xml --- a/macros.xml Mon Jul 10 16:42:21 2017 -0400 +++ b/macros.xml Sat Aug 05 07:27:17 2017 -0400 @@ -33,7 +33,7 @@ - + diff -r 10214bba6195 -r 8218f848c2b9 pick_rep_set.xml --- a/pick_rep_set.xml Mon Jul 10 16:42:21 2017 -0400 +++ b/pick_rep_set.xml Sat Aug 05 07:27:17 2017 -0400 @@ -1,25 +1,25 @@ - + (pick_rep_set) macros.xml pick_rep_set.py --version diff -r 10214bba6195 -r 8218f848c2b9 test-data/extract_barcodes/inseqs.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/extract_barcodes/inseqs.fastq Sat Aug 05 07:27:17 2017 -0400 @@ -0,0 +1,8 @@ +@HWI-ST753:99:C038WACXX:4:1101:1252:1987 1:N:0: +NACGGAGGGTGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGCGCGTAGGTGGCCCGGTCAGCGTGCGGTGACAGCTCGGCGCTCAACCACGAGTAGG ++ +#1=DDBDFHCFHHIIJGDHIJJJJIFGGHCGGGGEGIEGCHHHEDB@B8?;@AAB?DDGGEE@DDDDB>FDGD>@-:74=;A*74====?BBD@ -@L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG -+ -GGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHFHBHHECCHBDHFEHAE@@BDB=B@@A2D(5553DAAAAE@EEA>;)9;==A>7B@=@78;:82?C<><9?<>>>98=@ -@L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA -+ -IIIGIIIIGIIIIIGIIHIDIHIHHF@IIFIIIIIGIIIHIHGHBIIIGHHGIFEEEFHHHDEEDG>DBEFBDB@BB=EEBBBEGEDDBA>D@@@@BCBB;BD@@>:@@>B@?=1@@@3<9767=6>;;? -@L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG -+ -IIIIIGIIIIIIIGIIIIIIIIIIIFIIGEGGDIIIIIFFHIIGDGEGD>GDGIIEIGIIFH>GBDDGFGGEGBB2DBDDGBDEBEBB8?B>A@D=38=AC>?>@@?DD@>=?=478;+;BE8>B -@L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG -+ -IIIIIHIIIIIIHEEIIEIIIIIIGGHIHEIIHDFCIGHHHCEDBIEIEEHBBEBBEIHGGGGGDHGGEEGDG?<2A5DBBBBEBEBBB@BEDEBBBE;@@@B@@8:@B@@@@AE@@A0@>=<9:;9=7>:;==DBD=@DBBB=D6'=;;A=?=99=9.9.2,/2===79@@6@ -@L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT -+ -IIIIIIIIGIIIDGIIIFIIHIIIIIIIIIIIIIIEIFGFGGGHIIGIIIHBHIHGIGIHI@EG@EGFFIFEDFBC=;;2?-?=A? -@L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG -+ -IIIIIIIIIIIIIIIIIIIIIIIIIBIIIGIIIIIIIIIIFIIIIIIIIIIIHGIIIIIIIEDGFGEDDGDBG:@@ECGEFEBHBHFHBB@@GGBBBDBECBBGECBECBFEBDEBEBB@@2B4?<=?;A??A@A+>? -@L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG -+ -IIIIIIIIIIIIHGGIIHIIIIIIIHIIIHIIIIIGIHHIIIIHDIIIHHHDIIIHIIHDHGEHEHGDGHEDGDHEEFEHG,EDCDCF@EBBECB@@B6>@A@ECBBCBBEEA>BBB@@DBAB=@8@@ -@L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT -+ -GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHGHHHHGBHHHHHHHHHFHHHHEHHHHEFEHFGGFGAA>C=GGDBDEDEEBCBBEEBB>BD@BB@DBBBB=C>BCCB>EBB8? -@L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG -+ -HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGBGGGEGGGG@GGDGHHFGHHHHHHGGFEGBGGG3CE>EAABGDBFFEDFE>E>DCB@EFFBC>EDBDD+CAA>>9?=99DCC?ACABEBBEBEECEBGDECDEEEEEED8B>@@@??EEB*B71;2>7@?===@ -@L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT -+ -IIIIIIIIHIIIHIIIIIIIIIIIIBIIIFIIIIIHIIHHHIIFFIIIFEIFIIIHIIIIIHGIEECHEFIDIBC>GBFFFEEHBEGEBBEFDBCBBF8A??ABBD=B@@DBB??A;;>? -@L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG -+ -IIIIIIIHIIIIIIIIIHIIIIIGIFIIIIIIIIIHIIIHHIHHFIIIGIIIEGIFHIIGHIIIGHGIGIGHIEDBFAEHHFEGGDGECBC@BDB@ -@L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG -+ -HHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHGHHHHHHHHHGFGGGFGGEGGGGGGHHHHHGHHHHHHHHHEEEGCHHHGGGHHHBHGH>EEDBDEHHHDFBFG>B -@L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG -+ -IIIIIIIIIIIIGIIIIIIIIIIHIEIIIHIIIGIIIIIIHIHFIIIIIDIHHIGGIIIIHBHIFIFIIIHHIEE@GBGDDDD@EHEEEECBEEEDCFBCBBFEEI>@B@DBDBEEB9???,?@A=A=<=;*;? -@L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG -+ -IIIIIIIIIIIIGIIIIIIDIIIIIHIIIHIIIIIHIIHHIIIIHIIIHHFHGIHHIGGIEHDICDEIEIIEIBBBD@FBEEBEEEFDDBEFEBBBBEBBBBD?BB88<:<9?B@B@@@A?3@@@9?@ -@L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA -+ -HHHHHHHHDAGGGHHHHHHHHHHHHHHHHHHHHHGHHHHHHH@HHHHGHHFGHHHHHHHFHHHHGHHHDHHGHGGHGBC>DECEDB>6>>>A6>>;4>=6:=9=B:@ -@L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG -+ -GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEHHHHHHHFHHHEHEHHHHHHHBHHEHGHHHFGGGGBEEHEGCBGDCGEGGEGGEGECCEC@EE>B@A==@7,8=>;1=9A>>>;A -@L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG -+ -IIIIIIIIIIGIHHIIIIIGIIIIIFIIIHIIIIIHIIIHIIGIGIIIHIIFIIIGIIIDHGDIFIFEHIFBHBDFFFEIE8BDEDDEEFDDHECC@EABCEEBAD3D@BCCCEF>CBBBC8C?B@=@@<<>BB=@@B -@L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG -+ -GGGHHHHHGEGGGHHHHHHHHHHHHHHHHHGHHHHHBHHHHHHGCGBGEEGEEGGDBBADBGDFDEEGCGFEFFCDBBDBECCEFHEEEE:HB;A<::=?>=;EEEAE@>AC>,:.52?;:5255:,7@3:4:@ -@L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG -+ -IIIGIIIIIIIIGIIIIIIGIGIIHIIIFIHIIIIHHIIIIIEIEIHIDDGG@GGBGGGHIIIIGIFIGIIHHEEEGBHEHHFFGHGGGGEGDEBEDEACDDCABBDDB@@@BEBEBCCBDBBBBDEE?B@>B -@L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG -+ -GGGHHHHHBHHHHHHHHHHHHHHHHHHHHHGHHHHHGHHHHHBGHHHHHGHHBGGEGEDGBGGHHH>HDBGDGHHGHED>GB@GEGEGHFBHED@BDDDHFD@C>FCEFEHBB>@?:>??8826=8/:;<<:?>)>A -@L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG -+ -IIIIIIIIIIIIBHHIIHIHIIIIICIIIFIIIIIHIIIHGHHFEIHIEFIIBIHFHIIHIIGHEHGHGHHGHDBBICHEFHEGGDGEECGHEGDE>GGEDEGDDDBEEBEE@BBEBBDGDC=ABBBB?==-:==? -@L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG -+ -HIIIIIIIIIIIIIIIIIIIIIIIIIDIII3IIGGGGGIIIII@HIIGIIHGIIIIIHIIHIIIHIIDEGGEGGHHIHIHIIGDFIBFGFGGFGFEEGEFEF@EEEEFEBEDFDCD@BBEDE>EDCFBEDBEF>;A>:>AA8>>;AA -@L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG -+ -IIIIIIIIIIIIFIHIIIIGIIIIIFIIIHIIIIIIIIIIDIHIIIHIGIIHIIIIIIIIFHHIGHGIHIGEDBBBGBGHFHGCGHGEBEEIEEBEECEEHCHBEFBBB@BBBC>E@B?@@?@=@@BB;>@?>@?>A -@L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG -+ -DGGEBEEE?B,B?GGGGGGGGGGGGGGGGG>GGDGGGGGGGGGGGGGGGGGGGGGA>>G@>GDDGDGFFGD8FBB??>GDGDBA@AD;AAAE5?B;;4ADDBBB@B@D1.?8??-8::;? -@L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA -+ -IIIIIIIIIIIIHHHIIIIIIIIHIBIIIFIIIIIHIIIIIIEFIIFIEHFHHIIFIIIIHIHIEIHIHIIFICECIBHHIFEHDEGECBBHEDCBDFCEEBHBBEC>B=BBBCEBDA@BB>B>BB@CB=?@ -@L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG -+ -IIIIIIIIHIIIDIIIIIIIIIIIIFIIIHIIIIIHIIIIHIIIIIIIIIIIIIEIIIIIIIGIHHEIHIHHICEHIHHII@EHEHFEGDGHGEFEEFGGBEHBEEBBBEBEB@@>@=@B -@L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA -+ -IIIIHIHIEGGGEIIIIIIIIIIIIGIIIHIIIEGBGDDDDGGGIIIBEHIIFHHIIFIIGDBC@>EC3@?>@GEGG@@DBD@E8CC=BBDBEAC,38==:;=@?,3=9-<;68<===B -@L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT -+ -HHHHHHHHEGGGGHHHHHHHHHHHHFHHHHHHHDBGGFHHHHBGGDDDHHHHGGHHBFGFCGGFBEGGDGD>D>9ACACEEEBEEBBCDB@EF>DBB@C3*=@AA@=@BAEEBCCB@C -@L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG -+ -IIIIIIIIFIIIIIIIIIIIHIIIHHIIIHIIHIIHIIIFHIIFHIIIHFHIIDHHIHIIIIHIEHDIGIHGFDDCFCB@DEBFEFCFC>EDDBBBBBAAA?A@BB@E@?>-@A@B??@DB-A -@L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG -+ -IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIHIIGHIIIFHHIHIGIIIIHIIIIGHHIHIGHIECCGCFFIFFHDFGB=@@DBBBBBBDB?@EBBB=B>=B@BBBB -@L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG -+ -HIIIIIIIIIIIIDIIIHIIIIIIIBIIIGIIIIIGIGIHIFIHHIIIGHIIIFHHIGDFIEEIEFIDFHHGEGBHCFHGEGDGCDEBCCBBEEC>B@B>B8@BBB:B;???9@B>C@>;? -@L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC -+ -IIIIIIIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIHIHHHIIHIHIEIIFEHHIIIIIIIHIGIHIBDHEHGHHHGHHEGDBCDF>DDCBFE@?BB@B@78BB;@=@@?@=;A=????@:>=@=B -@L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG -+ -IIIIIIIIIIIIIIIIIIIIIIIIIGIIIHIIIIIHIIHHHIIIHIIIEEIFIIIFHIIIHHEIGGGIGIIGIGGDIEGGHGHDEEABCCEDBC@BBEBBDBFEEGABB@BCC<<<;:A0?A -@L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG -+ -IIIIGIIIIIIIIGGIIIIIIIIIIIIIIIIIIDIIIIIIIIHIHHHIIIHFIIHHIGIIIIHGEIDGFIIHIHFEIEHGIGHGGEG@EEBGEEBDEEEDGEHHDGHHGDDCGBDBB?DBD@B??A8A?@DBGGGGGGGDGGGGGGGG@GGGGGGGGGGGGGGGGGGGGGGGDGGDGCFF>?GGGGGGEGGGGGG8GGGGDGGBGCE>CECDEBEC?>,+?A=??==B@BBA<>>/=9?8?>>? -@L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG -+ -IIIIIIIIIIIIIFIIIIIGIIIIIBIIIFIIIGIFIIIHHHHIHIIIFHIGEIIHHIHHIH>IFIEIHHGHIBC>GCFEIEEHGEGDEEDGDBCBBBDDEEH@2@AEEB>BAA>>9>=AA77>>@? -@L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG -+ -BEEGGGGG3EBEDEEEEEGG3@>,EEGGADB??<16/459DFDGFFFDB15*9:/AAAAA(??;;FCCBDDD@>BBDBBB85>;A -@L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG -+ -BBBGGGGGDDGGGACA@,;<58;GDGGGGGGFGGBGGEGGGGGGGGEGEGEGBGEGGGEG8EEFEFEGFBFADCCEBDBFBEEEEAEBBBDD<>A8>>AEEBE@DBB8DB@B0>9)>>@=8@>:3:8@ -@L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG -+ -<@BGGGGGGGGGGGGGGGGGGGGGGDGGGGGEEGGGGGGGGGGGGGGGGGEGGGGGGBEGGGGGEGGGGGGGGBG2GBGDGGDEGDGFEGFGEGFFDEEGDEGEFDEGDDBDFC8FCBDDCCDDBFBC5AA>A>A8AAAA5@89? -@L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG -+ -IIIIIIIIGIIIHHIIIIIIIIIIIEIIIEIIIIIHIIHHHHHHHHIIGHEHIIIHIHFHICGHEFEIEIHEH@=BD@DEFECB:BBBCBCFCBBC=CCC@8E@CBBCBBBBBD8D@;AA@??BDDEGDBAAD=>@@@ -@L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG -+ -HHHHHHHHDGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHHFEHHHHEHHHHHHHHHHHDHHHHGGHGHGDHHHFGGGHHHHHHFHGHGHGCEE2EBA?CB@B@@BDBBB=B@=BBBBBBD7BBB -@L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG -+ -GGGGGGGGDGGGGHHHHHHFHHHHHHHHHHHHHHHHHHHHHHHHGHEHHHHHHHHEHFEFBGGHGGHHBEGEGFHBEHHBHGHHDHHHGHGBHC8>8?ECDEEEECB@/:::;5==>>A -@L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG -+ -IIIIIIIIIIIIIIIIIIIHIIIIHHIIIIIIIIIHIIIIFGHHHIGIGHHIIIIIIHIIHHIIGFFIHHHGICHGHGHGGHEDHHGDFGGGGGGDB@BCCBDABE@E@BBBBEEBF@?A>?==B@/=?@?>;@BB@ -@L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG -+ -IIIIIIIIIIIIIGHIIIIDIIIIIGIIIFIIIGIIIIIIIICGGIIFBDGG@IFFIFHGHIHDBECEHGHGFFD8FCGCGGGGFGEBDEEEBEEGEGBB>BB@BBCCA=;??=A@=?:>@??:@@(@ -@L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC -+ -IIIIIIIIIIIIIIIIIIIDIIIIIHGIIIIIIIIGIIIFIHIIHIIIGIHFIIHIIHIGHGGHEHEBEEEFCGEGDBEECEBBCC<@BDBBEDBDBD@BCBCDDDADB@==@?@B=@@B3B=@@ -@L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG -+ -HHHHHHHHHHDHFHHHHHHHHHHHHGHHHGHHHHHHHHHHHHHHHHHHGGGG>HHHHHEDGDDGFGGGDGGBGEEGBGG8G@DEGGEFEFEBBBDBBD@>?:;??=?B -@L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG -+ -HHHGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHHGG1D>D;A9A8C -@L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG -+ -HHHH@HHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHHHHHHHHHGEHHHFHEGEDGDDDGGFFFDDABEEEDDCFGEEDBCAEBEEEEE@EBBEBBEEEE8B@@:<8==<=BAA>A@:@8@ -@L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG -+ -IIIIIIIIIIIIIIHIIIIIIIIIIHIIIIIIIIIIIHIHFIIHIIHIHHHIIIHHIHHIIIHIGIHIFHIFHEDBIDFFEHHGGHHEFEBIEFFCEED>EDEBEEDBEEHDBEFEBDGEEDDBFEDEBCBBB;CBBB -@L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG -+ -IIIIIIIIIIIIIHIIIIIIIIIIIHIIIFIIIHIHIIHHIHHIIIIIHFIIGIFDAGIIHFHHEH@IHIIGIHGGIEHGHEHGHFHGEEEIHHEEE@EEEEIEFHBEDFGDFGGEEDEEBB18A@@@B@88@ -@L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG -+ -IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIHIGIIGIIIIGIIIGIIIHIIIIIHHHIIIHIIIHIHHIIIHHGIGE>FEEEFHEEGGGGCHEHGGEGDEEGG@GDDEDGEBEGDBEEEDEBEBBDDCCDDC>DCC@C?CBCE@BBBB>9@<>?;@+;CCEB>B;/=?=BCBCB>BB>@@B<@?<85*928578=@B -@L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG -+ -GGGHGHHHHHHHGHHHHHHFHHHHHFHHHHHHHHHHHHHHHHHHEHHHEEGDGHHHHHHHHHHHGHHHHHHHHDGGHGHHHHFEHGGBHBEHEGHGHGGHGDHAEHEHBBDBBBBC>BEEEBBAEAAEEBEAB><@B -@L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG -+ -HHHHHHHHEHHHHHHHHHHGHHHHHHHHHDHHHHHHHHHHHHHHHHHHHHDHHHHEFHHHHH@HHHHHEHHEGEEEHDHEFHEGHE@EHGGHGC@B8DEEECEBEED8;?=??DBCDBCCCBDBBCECGGHH@GGCFGGEGHGB@BBBEEEEH@BBCCB@B>CE=A@@=:==? -@L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG -+ -EEEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGFGFGGGGDGFGGF>EGEGEFED8DGDEEEBGDGBDFDBDBDEE8>+>;;49;? -@L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT -+ -IIIIIHIDIIIIIDIIIIIIIIIIGDIIIEIIIIIIIIFFFIIIHIIIHEIIIIGFIIIGIIHIEHEIFDIFIGGGHEIEDHCGHGHHDEEIEEEEEEGGG>GDEDBB>ADAD@A;>4<=<967:<<=:? -@L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC -+ -HHHHHHHHHHHHHHDHHHHHHHHHHHGHHHHHHHHEHHGGBGGGHHHHHHHHHHGHHHHHHGEGFFGFFGFDFEFHFDHEEHBC2BEC8=??ABBB@D@@>BBBB@5@77==1CC@BB -@L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG -+ -GGGIIIIIIIIIIIHIIIIGIIIIIDIIIGIIIIIIIHIIIGIIGIIHEGGGCIIFIIIHIIIIHGHIHHIEHHEDDHFBIDFFHHFEGGGHG>EEBEBCEBC@BBBB=69=,:186:EBEBC;;@:@ -@L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA -+ -IIIIIIIIIIIIGIIIIHIIIIIIHHIIIIIIIIIHIIHHIIIIHIIIHGHHIIIHHIHIIIHIEEEIHIIGIFCDIBHHI@HHGEDD>B@DBEBBBEEEBEFCEGEEBE>>)>@EDCC@B<>:5:6:>68>0A;9>;:@?<>??@B -@L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG -+ -GGGHHHHHHHGHHHHHHHHGHHHHHGHHHHHHHHHHHHHHHHGHHHHHHHHHHHGBHHHHHBHHHHHHHFHGHBHHFGEGEEBEHHHDDDB@GB=?=?EEE>EBD3=@AABDDD>BBCAAECDCDB@BB@:C@BB@B=0=0>?=>@ -@L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG -+ -IIIIIIIHIIIIHIIIIIIIIIIIIIIIIIIIIHIIIIIIHIFIIIIIHHIFIIIHGHHIHIIIHHIIFFICIB8BDGD@DBACFEDGCEEBFFFCBBBEBEBAA;8?>?AAABBCC@BB>>C@DB@B@7@@@83;8@89<7+=8=(=5?9? -@L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG -+ -<@@GGGGGGGBGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGBGGGGGGDGGGB@>ADAEEE@D?A=<=@B@@@B@B>BBBBCA@@)>@<4>1>@@>;>A4:@A -@L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG -+ -IIIIIIIIIIIIIIIIIIIHIIIIIDIIIIIIIIIHIIHIIIIIHIIIHDIFIIIIIIIIIIHIGIGIHHIEIFFEIEIHIFIGGHGHDHHHGHGBB@@BBB=BBDB?@B:BBBEEECB??A4@4?==? -@L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG -+ -=B@GDGGGEGEGGGGGGGGEGGGGGDGGGGEEEGGGBGGGGGGGDGGGGGGG@EEBBE???DBGGGEGBGGDGGGGBC?0?BDGDDGCBDDDDDDDDEGGFDDDAG+(2:39DBAAA -@L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG -+ -IIIIIIIIHIIIIIIIIIIGIIIIFFIIIFIIIIIHIIIHEIIIHIIIHHIFIIIIHIIIHHHIGHHFHIHEIDBBFGHFHFEHEIIEBDBEEDCDEF=DBDDCBD=D@BDEBDFCC>B@B4B=@=>@=BB>8? diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/forward_reads.fastq --- a/test-data/split_libraries_fastq/forward_reads.fastq Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1000 +0,0 @@ -@HWI-EAS440_0386:1:23:17547:1423#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGATTGGTGTG -+ -hhhdHddddddddfehhfhhhghggfhhhfhhgggfhhgfgdfcfhehfdgfhggfggfggffgddfgdffdgdaagaaddcbdccc]a^ad__a]_____ba_`a`__^__\]^OWZR\Z\\WYTZ_U^BBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:14818:1533#0/1 -CCCCNCAGCGGCAAAAATTAAAATTTTTACCGCTTCGGCGTTATAGCCTCACACTCAATCTTTTATCACGAAGTCATGATTGAATCGCGAGTGGTCGGCAGATTGCGATAAACGGGCACATTAAATTTAAACTGATGATTCCACTGCAACAA -+ -US[QCQSZPHNYIa[^aaccaac[]accBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:14401:1629#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGGGTTGGGGG -+ -fffbF`bbWZZ]Zggggfgcgggggddggdgggggdbgdddgbgeggg`ffdggeabba`aaad]]]d\]`]`[]]aWaYa\aa`a`_````_^]^^^]]]X]_``____``BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:15259:1649#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGAGTTTGGGGG -+ -hhhdHcdd^bbbahhhhhhfhhhhhghhhhhhhhhghhhgehchfhhhgghghfhhhgehgaffaecfdggdh\bafadddddgddfdbc^]a_\^_a``\X`^_`]`aaabcaYbY_^^X]^ZY^Z^a`_^aBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:13748:2482#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGCGGGATTCGTGGTG -+ -fffbF^bbS[T[Uggggggg__gffddfggeggggaagfeefbfaaff_cfacfeceddgggegdgdgaggddcddd`a_aad`ddadd`daaWY]]Yddda_]_]]a_Y___X\_]YaY]\]WYNV]\]_BBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:6532:3028#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGAGTCTTGGGG -+ -hhhfHdffacdcchhhhghfhhhhfehhhchhhehghhgghhgehghhffghhhgghghddgggfhfgdhhdea`Wf^dddddbgagcdedgcfddbcddaddcefb_addddd_daaaaaaa`ca`aX^Z\X_^^^^BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:8677:3027#0/1 -TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGCCGGCACATGATACTGTGAGACTAGCGTAACGGAGGGGGAACCGGAATTCTTGGTG -+ -ggg`Fbbbdeeeegggggggggggfgggggggggggggggggfgggggggggfgggggggf]gfccfcfbfbdged]ddda_dedaa]aZc__BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:5678:3052#0/1 -TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGGGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTGGGG -+ -hhheHeeeaaabbhghhhhhhhhhghhhhghhhhhfhghhghhghhhhdhehhfghhaehhgghddhhhhhadffffdb]Z__Z^_`^daadda`c`Z\T[\\]\RJ]YS[\X_a]]P_XM\T\^\^^BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:11889:3171#0/1 -TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTATGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG -+ -hhheHddeeeeddhghhhhhhhhhhchhhhhhhhhfhfdddffhgghhghgehhegghhhhffhffhhg_edaecdcc[dabaadddaadaaeY\WQX^]]^^``RZZ___a__aa_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:2112:3374#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGTGTGG -+ -fffdGcddZZ___ggggggggggggggggfggggggfgdggggeggggeggggggeggdggeggggggdggdgcaaf\^_b_b_gdedd_eedR^Z^^`dcRb_P__R^\[]^c`cbadaaab^da^aBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:5900:3393#0/1 -TACGNATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGTG -+ -hhheHeeea\aaaghhhhhhhhhhhhhhhhghhhhfhgggghhhhhhfcghhhhgfhdgahddfbddfcfca]daed]cfcdcb]bbb^]_^bUZRYW___^_abaV_^SYYW````]^_[O_^```Z\RS^BBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:15468:3455#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGGGGTGGGGGGGATTCGGGGGG -+ -hhhdHaddddedehhhhghhhhhhhchhhghhhghghhgfghhddfdhggfhfhehghhfhgghdhehghhfgaaaf_cacaadaadbb`bcaacabcdbecc`ddaaa_aabacaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:4405:3490#0/1 -TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACGGCTTTTGAAACTGTCCTGCTTGATTTCAGGAGAGGTAAGCGGAATTCCTAGTG -+ -fffdGdcdbabbbggggggggggggggggggggggggggggggfggggggggggfggWffcfdgdggggggdfdeWadggededacgegdddgcd__da_c`XebdadedaedaJaaaW``_RZW^[W]Q^BBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:4674:3593#0/1 -TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGAACCGGAAATCTCGGTG -+ -gggUDW_Y[a^^aggggggggagfffffcffeeccffabbbbcfefffdeggdggeggggddda_cf_bY__`bbbaa_cccaaL^aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:15912:3842#0/1 -AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAAACTATGGGCTCAACCCATAAATTGCTTTCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTAGGTGGAATTCCCGGTG -+ -fff\Faaa`babbggggggggggggggggggggggggggggggggggfggggggg`ggcdfdegegggfafdfgggdgggggfa_eggaddbdaaf]`fecddgdddb[``a\aaa\`bddbdacb]caacBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:15732:4249#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGTGGGGGG -+ -hhhdHdee`bbaahhhhhhghhhfddffhehhhhhehhhdhhhaeddhghfheh_gghghedehggdhghggfaacfaedf_fabddaaa_fab_```decdhcegdW[_b`bdaeaa^b]W`[`]``BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:17444:4527#0/1 -TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGATGTGAAAGGCTGGGGCTCAACCCCGGGACTGCATTGGAAACTGCCCGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG -+ -hhheHdedfffffhghhhhhhhhhfffhhhghhhhfhfggghhghhghgggfghhhfggfdffgachh]ca_aaabdd_cdead]_aa_]]_baaa_a_a_]_\YJWZX\U\Z^^^U_a``^a\aBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:10725:4873#0/1 -TACGNAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGTG -+ -gggdGbcdb^b`\fffffgggggggdggggggggggggeeefffcfdfgggggggggggcgfga[_cfdfeb_`_bbdddeefdbdccdgaggggdafcddbgdeaeaaaaabdgdda]]b_bccecacaaBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:11691:4945#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATACGTGGTG -+ -hhhbHbbbcdddchhhhhhhhhhhgghhhhhhhhhhhhhgchhghhhhhhhfhhghhhhhhgghghfhghhdhdcdhcgghghfbggdedghedcdah]dbaacdgddcaeee]dd_ac_``_TT[T[Z_XZV_^W^BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:4723:5217#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACCGTAGGGGTGGGGGGAATTTGTAGGG -+ -NRRKD\ZQVUVSTcccccccbccccccccc_cccccaccccccccccc_ccc_bcc[[cccccccc[caccccc^ccP]Z`Z_ccccccccccHQGYVa_acccccYc_ccccI[Va]\\^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:13608:5705#0/1 -TACGNAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGCCTGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGAACTGCAGCGCGAACTGGCTGGCTTGAGGACCAGCAACGTGGGCGGAATTCATGGTG -+ -hhhbH`ba_Z_a]^ffffhgd]hffdffhhhhghhbhhdedfffbfef_acfcdddadgaaeeaRcaa```^`___ca_aaabcbcabdccdcacaaa_Zb__\__[___Z]_aabBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:5195:5979#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGATGTGGGGGG -+ -gggbF^bbSa\_\gggggffaefffdafgggggggdggeedfafa[cfdgeggggggg]ggddgdgegdggegabad`dbbbcdddbdd`adT]TY]_]_Z]`aae`c]]bYd]Z`WWaa_]aBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:1372:6057#0/1 -TACGNAGGTGGCGAGCGNTGTCCGGAATTATTGGGCGTAAAGGGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT -+ -fffcGcccZY]]Y`abaEccaccfffdfffWffffffcfaffafffffcfffcafbdfc_ccefccfc^W^^abbcacYYX^Vccaccdc`b]^ZP^X^M^Z^`W]W]a_aZa]^MX]]]M]W`_XW`BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:2305:6385#0/1 -TACGNAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTGTGTTTGAGGTAGGCGGAATGCGTGGTG -+ -hhhaH`bbddddchghhhhehhhhgahhhehhhfhhhhddcfffdhfhfcffdhhefhhhhhghghggehebh^[\d\fcfacggdddfffchb_dXc``_afaa_aaadbcddadaa^\RH\JSISO___Z_BBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:17333:6563#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGTGGGG -+ -gggdGaddabbbaggggggcgggggacgggggggggggggggggggggeeggfgggdggeggfcaacfecfdfgeQgedgggefedfegffgfgfbffgffcfadg]daadc][aa_aaca_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:1091:6583#0/1 -TACGNAGGTGGCAAGCGNTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGNTNNNNNNCCNNCCTGCATTTGAAACTGNAGTNCTTGAGTGTCGGANNNNNNANCGGNATTCCGTGTG -+ -\`^WF`UY^RW[\ab`aEacdcafffceffaccffdfffffdff]fRfd^eeeffccfReaaaebbKef`ffff]fafda[ddPCVDDCCCCYFDD]XZ]\VZfRcc`TYZ_DQT^DU`^`O^c[cfBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:16169:6630#0/1 -AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG -+ -ghheHeeeaddcdhhhhhhfhhhhghchhhghhhhgghhhghhghhhffehfcgghghggdfghdhh_dddbdddghbfggddc_dfadad`ddaba_gddadeddabaadeaadbb``eb]caaaabaad]ba_W^_Z_ZQZR\UZ\Y[TY -@HWI-EAS440_0386:1:23:14554:7030#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTTGGGGTG -+ -hhhbHb`beeeeehhhhhhhhhhhhghhhhhhhhhhhhhhhhhhhhhhhhhhdhghhhhhhhghhhhhhhhhhggghghghgfgghggdgfggdcgghgh_dhgghdgcdadd]`b_`fddadZ^_W`\^`]b`_b[`BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:14518:7351#0/1 -AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG -+ -dggdGdcdadddcgggggggggggggggggggggggggggggggggggggggggfggggggggfggfgg_gdgggdffegggffbggecddddababdgfffdff`da_acfa]a]`]c^c]daabaaa[c_aYY]]_T`XQNU\TUU]_[a -@HWI-EAS440_0386:1:23:17440:7360#0/1 -TACGNATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGCGGCAGTGCAAGTCAGGAGTGAAAACTTGGGGCTCAACCCCAAGACTGCTTTTGAAACTGTACAGCTAGAGTGTAGGAAGGGCAAGCGGAATTCCTTGTG -+ -gggdGdddddccdggggggggggggfgggggggggggggggggadddffdfffgggffegggdggggg_ddb_ebddeebgcgfggff_dePdaaca_dddgdff[daadccaa]aaaaaKaa_aT\a\Z[^RY\\\\]_BBBBBBBBBBBB -@HWI-EAS440_0386:1:23:11290:7687#0/1 -TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGGACCGGAATTCTCGGTG -+ -hhhdFeee^aaaaghhhhfdb_dfddcfghfhhhhfhhhfhhccffffhhhhcfhhhhhhggefdff_fefefdddbe[ddedc_Zaa^[^^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:1465:8235#0/1 -TACANAGGTCTCAAGCGNTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGGGAGACTTGAGTAATGGAGGGGGAGCCGGAGTTCTCGTGG -+ -cccLD]YZVZ]]]aaaaDcccccccccccccccccacccccccccccccccacccccccccccccccccccccccccbTTP]]cBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:3176:8324#0/1 -TACGNATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGATGTGAAAGGCTGGGGCTCAACCCCGGGACTGCATTGGAAACTGCCCGTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG -+ -ddd]D`__Z^Y]UfffffffffffffffffWfffffafffffffdffffffccfffff^ZO^^dad]d^dd^dcfcf[IZ`Z^ffcfededa`^WZZWcfaaaXTWYH^^^^SWM^X]cbbacacb[c[H^BBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:12535:8807#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGGATTGTGGTGT -+ -hhhcH_dcaddacghhhehghhhghdhhhdhhghhdhhdeeeegfhggfgffgdghgghfffffcfggfgedfaaad_dcdcadaddab`ddaddaacaaaae_ca]aZ__ZaaaaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:14562:8883#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTCGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTTGGGGTG -+ -hhheHdeebbbbahhhhhhfhhhghghhhhhhhhhhhghhhhghghghehhfchhhhhhhhhhfghhhdhhehdc]hehgggehfaffddahdececcdd]dgac_bbcabcddbgcd]ca_aZ^ZOZVV[Q[a_aBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:5451:9226#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGCGGGATTCGGGGTG -+ -hhheHeeedeeeehghhhhhhhhhhghhhhhhhghghhhhhhhehhehghhhhhhhhhghhhhhfgghghhgheedfeegheggfdhfcdahegddgefgcdfdfg[ca_dddcdcea[dbcbaadaedaaaa]^^aBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:19204:9448#0/1 -TACGNATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCAGGCGGTTTAATAAGTCGAATGTTAAAGATCGGGGCTCAACCCCGTAAAGCATTGGAAACTGATAAACTTGAGTAGTGGAGAGGAAAGTGGAATTCCTAGTGT -+ -b^^aD^aaccccccccccccccccccccccccccccccccccccaccccccccccccccccccccccacccccacccc`cbcbc`aaacaa_ac_a__a^accca[_[X__Z_NRMJT__Z_VX[X[aY_BBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:13161:10184#0/1 -TACGNAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATCTGCAAGTCAGTAGTGGAATCCCAGGGCTTAACCCTGGAACTGCTATTGAAACTGTGGGTCTTGAGTGAGGTAGAGGCAGGGGGGATTGCCGGGG -+ -hhhaHddccdccchhhhhhhhhhhhfehhhhhhhhehhhhhhhhghdhghhhhhhhhhhgchghdhfcddddf_acffeghheefghdgagbhhgddhegdggehhaa_]`b_dedab`[`__adaabY]^\_Z^Z^BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:19192:10519#0/1 -TACGNAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGGCTTTTAAGTCTGACGTGAAAATGCGGGGCTTAACCCCGTATGGCGTTGGCTACTGGAAGTCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT -+ -dhh[Ga\\daacchhhhhhdhhhhhhhhhhhhhfhghhchdhhhhhhhhgfhhhhhghhhhcchhhhhhhhhhdhehhghchhhghdh_fcWcfaccf``d`]UPIWMYZSYW]\L[Q\[Z[Va_a_[^_BBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:17370:10963#0/1 -AACGNAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAAGTGAAAACTATGGGCTCAACCCATAAATTGCTTTCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTAGGTGGAATTCCCGGTG -+ -fggdGeeedccccggggggggggggggggggggggggggggggggggeggg[gggg]daafcffedfcaafffeddaefdddcddcdegggcffWdafddedcgg]g]caff[aac[cccf]c_aR_a[cc[ca]aacV^BBBBBBBBBBBB -@HWI-EAS440_0386:1:23:8386:11532#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGGGGAGTTCGTGGTG -+ -hhhbH^ba\aaaaefhhhhghhhhhefhhehhhhhghhhghggehgggeghdghhe`gfghgggdgghfggfgfdcgdffgfccfbfeddcfcfccdfdfcdfaee]a_]\\_]^Y^\b__]]\_aa\_\a_aBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:10215:11663#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGGGGGGGG -+ -fffbF`bb`bbbaffffffffffffefffffccfffffffffffefffefffdffefffefedfdeefedddf[aTc_ddedecdddddddcaddaadccY_cbddad]aaacZQ^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:15454:11726#0/1 -TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCCCATGATACTGCGAGACTAGAGTAATGGAGGGGGGAGCGGAATTCTGGGGG -+ -fffbFbbb`abbagfgggggggggggggggggggggggggcgeggggfe[fffgggfeba`b`fcaccfacafccacaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:6589:11781#0/1 -TACGNAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTATGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCATGGCTAGAGTTTGTCAGAGGAGGGTGGAATTGCGCGTG -+ -gggaFaaa^\\aafffffggcggffd_fgggggffefffcfffggggfgggggggggggggfafcfdf[dfdcddddfbfdeddddfbcc_ff_b]`abb`[bgegd_cddac_ggdddcc_[]``]]X]`Z]^VZ\\W_\OIRTEWWV^X^ -@HWI-EAS440_0386:1:23:3256:12101#0/1 -TACGNATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGGAATTCCTAGTG -+ -gggZE_X\\a\aaggggggggggggggggggggffefefddfffdfffggggggaggggggg]fdfefdegdgbacg]bddbdd`aaV_^aa^TXSOU__\_aaaa\_`aaa^U]\_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:5860:12157#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTGGGGGGG -+ -hhhdHadd\```ahfhhhhghghhgffhhghhhfhfhhggfhhdafdfeehbhhgfghfhgffhffdhfggfgddcgcfefbgaffdcdaaedb_aabdfcdcdefcddae[c`c_`\acaabcdccfaa^^^^FX^BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:8148:12187#0/1 -TACGNAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT -+ -hhheHdee^bb``ghhhgfadefhgdahhghhhhhghfhgghhghhfhgggfdgffgggfhfegddgggadagdabeecdaeab`dacaaaaad__bd__b_aa_\_\SY\[YaaZaa_a\_\a][^aXXFX\Z\Z^\_BBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:14399:12333#0/1 -TACGNAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCGCAGTGT -+ -]]^ZE^^^^bbbbggggggggggggggggggggggggggggggggggggggggggdggggggggddedcacacbbfbdddfcdb`ddddadQda_Rcaa]aaac`[___a`[_^ca^abb]b]aW\]aX_W`BBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:6966:12378#0/1 -AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG -+ -^[[YE\Z[\\YV\ffffffdfffffffffffffffffffffffffffdfff[]dd_]dcc[dcffcdfcfffffdf[c^^a[affdfffff[b]a^^^fffQad^Ya[a^adW`````cccQfZZIP^BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:5056:12637#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGGAATCGTGGGG -+ -gggeGdeebbbbagggggggggggggggfdffcffeffgggggggggggggggggfggggggfdcddfdfcdfbbafbdefcdgfefdcb_eabbabdceacfadda_bad_dbad_a]`]`Z\aa_`_d___U_]Y_BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:2628:12653#0/1 -TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGGGACTAGAGTAATGGAGGGGGGACCGGACTTTTCGGGG -+ -cccLD[[]]bbbacccccccccccccccccccccccccccccacccccccccccccccccccbc_cc_aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:3303:12826#0/1 -TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAACCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGAACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG -+ -hhhbH`bbbbbb`hhhhhhhhhhhhhghhhhhhhhehdgdghghghhhgghghhfhghgghefgddheeggagdcdgefdcfcd[ce]aaaaadcaX`aaadacacWa^Z^]Zaa_aR^]^VZZZ\\^BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:11685:12866#0/1 -TACGNAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGGG -+ -hhhbHbbacccdchhhhhhahhhhhhhhhhfhhhhehghhhhfghhhheghhfhhfhhhghh_edeeheghdef[dfcafaf_cdeeebdbfcb^`cb_``__ddccaffca__aaaada_a__^``[TOYPZaaZ`_U`__^BBBBBBBBB -@HWI-EAS440_0386:1:23:3932:12883#0/1 -AACGNAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAAGTGAAAACTATGGGCTCAACCCATAAATTGCTTTCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTGGGTGGAATTTCCGGTG -+ -accaD\aaZZZ_Zcccccccccccccccccccccccccccccaccccccccccccccacc[cbcbcc[Z[][^ZZUYZcbbbbcccbccaa`_bc`acabcbBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:3535:13218#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGGG -+ -hhheHbdd]````ghhhfhehghhfdhhhdhhgghdhhfdffgddgehdbhdheecdhffhfegff`gdgddga\_c_dcdcdddccbbaadaaaaacdda`c`ab]aY\_a__aa^_aaa__]_a]bZX]T^_Z_\ZK^BBBBBBBBBBBB -@HWI-EAS440_0386:1:23:6909:13409#0/1 -TACGNAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGTG -+ -gggeGddea[aa\fffffgggggggbggfeffeggggggggggfdf_fdeeffggfgafcfdd[caafggggecdggggfgggffggcdadgggcdcgcdaagddb]ba__]cba_bacdbcdcc`aabaaVJ____\Z_BBBBBBBBBBBB -@HWI-EAS440_0386:1:23:6191:13528#0/1 -AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG -+ -fggdGadd`deeeggggggfgggggffggggggggfgggggggggggegggggggggeggggggfgggeagdggfbggeaggbdbddaaa_dbec_cddddddfdccddd^fccce````badaa_aaa_aV_`Z]]]Q\]PW\\\\V\\T_ -@HWI-EAS440_0386:1:23:17158:13561#0/1 -TACANAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGCACATGATACTGCGAGACTAGGGTAATGGAGGGGGGAGCGGGCTTCTCGGTG -+ -eee_D]``\aaaagggggggggggggggcgggggegggggggaggggggggggfgggggggffdddfafacacaYaaaad[bb`ZZ]\a_bY_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:19363:13593#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTATAATTGCAGTTGATACTGGCGACCTGGAGTGCAACAGAGGCAGGCGGAATTCGTGGTG -+ -hhhbH`bbeeeeehfhhhhghhhhhchhhfhhhghghheehghfhfhhhhhc_hhgbhhgfgahhggghhgfacaafbddgdhbeagabaafecccadd_dadd\bad^^__aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:10747:13717#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTACAGTAGAGGCGGGGGGGATTCGTGGGG -+ -[`a_Ea_^ccaccffffffffffffffffffccffffffffffafffffffcfffff_cfffcfefffffffffdeffffffccefecddefdddedceaeafeff_caccccJS^ZWZYOVV]``]bYaaYa^BBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:11158:13762#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTCGGTGGG -+ -fffcGdcc`abbbggfgggggdggggggggggggggggggf_fgggeggggg]gggdgcgfggcgggdfffdfbdbcdgegadgfgdbedageddaddgecggbeaaeabcdbc__dW_aT]_]_V``__BBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:8303:13856#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGAGTGTGGGT -+ -fffdGcdd\aaaaggggggagggffddfggggggggggggggcggggggggcggfeggeggggggffgcfecfdbdddcccacegfgdeffeccdddefgfdf`ggadbaddd[Z`]\`b`W]]]\``ba__BBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:19671:14034#0/1 -TACGNAGGATCCGAGCGNTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGTG -+ -fffdGddd\aaaaacddFededegfagggggggggggggggggcffffggggggffeggeegggggffgged_```b`acfccabdcaaaWfa]_aaaffbdfc_[a]]_b`]_d]]__]S]`aa]abBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:3839:14047#0/1 -TACGNAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTCCTAAGTCCATCTTAAAAGTGCGGGGCTTAACCCCGTGATGGGATGTAAAATGGGAAGCTGGGGGTTCGGATGGGGAAGGGGGATTTCTTGGGG -+ -ddddGdddTVY]ZfffffffffffffffffffffdfcfffdfffffffffffcffcfQ^[dadfce]]a]adW````]cacce[cf_cccaaO^U^Z^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:10766:14550#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGGG -+ 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-TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTTGGTGTG -+ -fffbFbbbaddccffffffafffffefffffccfffffffdfffdfffeeffde_ffffdffefdeefdfdefdddfdfdddddcdddbcafddddddddddd`e`bda_a_[ddd_cbdbOb_]\aa\aa]aaaaJ`BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:18778:15286#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGGG -+ -ddd^E_a\addccgggggggggggfcgggggggggggggfggggggggfeffcggggggggfffdfdfgggggdddgdgggdgdYdcdbaadaddddgffdcgaggad_a]aaccfcc]cc_a_\__a_\aTaWS^BBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:1172:15688#0/1 -TACGNAGGATCCGAGCGNTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTGCAGTTGAGGCAGGGGGGAGTTGGGGTG -+ -W_WQF^NN]QN]NcZROEQ`U^NXX\XX[f[Qbe_f__d[^V``dTdVffYfb`efddafY_daff^[feefcfWccffffffafafdfffbfa\dddfaccffffW_ccddcKaaa[Y\aK_T\`W_V`bZ^a^Vb^BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:10432:15754#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG -+ -hhhbH`bbaddcchhhhhhghhhhhghhhhhhhhhghhhgehhhdhdhddfdfhhddhhhhgghdeehghhggdccheeggdgadada``^d`aa]aaddbbe_ddad]^b`badaaa_dd_bad_]da`_\_^\[_^BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:15265:16137#0/1 -TACGNAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTGGTCGCGTCTGTCGTGAAATCCTCTGGCTTAACTGGGGGCTTGCGGTGGGTACGGGCCGGCTTGGGGGCGGGAGGGGGGGAGTGGAACCCTGGGG -+ -ghhaHcdc_aacchhhhhfcfffhfhahhhhhhhgfhhghhhgghggghhhghghhghhgccdfcaffdaffddhdhd]ddffa_cc_[[```hea]e^^_d_]a^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:3853:16425#0/1 -AACGNAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG -+ -gggdGccddeeedgggggggggggggcggggggggdgeeedffegggggggggegegggdgfcc_ffcccaacaddgeeggddadagd_abcddaaaadc_gaaebaaaaac_dcbb``bb_aaa_^\YXZLUa\\__BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:4837:16715#0/1 -TACGNAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGGACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTG -+ -hhhdHaddcaccdhghhhhhfghhhhdhhhhhhhghhhffffafffffhhh]hgggghcdhhchfhheghhfdgffeggghgadddggde]fc_`Qcb_acaadce^ddaaadddaba_b__bedddd__aOa__`BBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:15530:16858#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGTG -+ -fffcGccc`abbbffffffffffffafffffccfffffffffffffffeefefffcefdfdfffcccfbffcfcccfcfdddcdcedb]bbebacdd[bcaacda[addbdc_WZ`ZZab_]bYZ`XaYaaBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:10039:17071#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGGTTGTGGTG -+ -hhhdHcdddeeeehhhhhhhhhhhgfhhhhhhhhhghhhgehhhfhhhggghhhhghhghggfhegdhghhfgdcbeahdgdcffffbdaagdcccacbae[aaddaba]dccccdccaaa_aQ][Z^\`^Z]_\_aBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:12821:17733#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAATCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCGGAGGGGTAAGCGGAAGTCCTAGTG -+ -gggeGdeeacddcggggggcgggggeggggcggffbfbcacffgggggcgff]fe_fffc]]ac_c]ccfc_abbefd^`\^\aaac_aa_afb`W``aaa[a_ca\]]]`]]acK_\\W\N]_d_aa]ST_BBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:6118:18575#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGTGGGG -+ -dffbFbbaUZZ^Zgggggg_gggggg[gggggggggagggfgggggfgafffafd^bbff_fffed]f^`dbdcccf_d_b^dfffddgfgcbceeeeggeWgf]cW[cccf[X^W`_accaceeaac]`b]]c_`a`BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:5450:18847#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG -+ -hhh`Hbbb_aaaahhhhhhhhhhhfhhhhdhhhhhhhhhhhgghghfhhhfhhhhghhhhghhhghhfehhghfgcfdhgggggfhhgggdg_addbdceedecggdg_dedcacaaa^[[WSPZZWW[X[XX^ZW^^BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:2535:18871#0/1 -TACGNAGGATACGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCAGGTGGTCTTGCAAGTCAGTGGTGAAAAGCTGAGGCTCAACCTCAGCCTTGCCGTTGAAACTGTAAGACTTGAGAGTACATGATGTGGGCGGAATGCGTCGTG -+ -hhhaHdbacddcchfhhhhchhhhhahhhhhhhhhhfhhhdhhhhhghhhhhghhhedhggghhhfafdfhhf^_^ccdabbdhcghheghhh]fcfchhdeaccfcW`cffcb^K[^bbb]d_bW`R^d`R`fccaBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:4582:18950#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG -+ -hhhbH]b`^``^`ghhhfhhhfhhhdhhhfhhhhhfhhgffggffgfhdffeggdghgfgefdfcffgadddeaaad_dddddadadacc`dcdccddaa`cd_ad_aUWQUT``_\]_a_\\\__\_ZZ^BBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:15559:19608#0/1 -TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGGGGGG -+ -hfhaG_aacdddchhhhhhhhhhhfghhhhhhhhhhhhgghhhhghhhgghghhhhhhhhhhghgfghhhhfhdechdhegdefcfff_abebgbdaeada_dadd_e_]a`cWXX[P[[WVVNXJOVZTYTS_\Y__BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:23:9787:19631#0/1 -TACGNAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCACTGCAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTAGAGCTAGAGTGCTGGAGAGGCAAGCGGGATTCCTAGTG -+ -gggeGeeea`ab`ggggggggggggggggggggfggggggcgggggggfggggddggggeggggfgdgfdgdgdddaadaaaaaV_b_aaaLa\__\O]_]\GZUOWO\S]\S\U\ZZ_\_^_\aBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:17851:2016#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTTTCGTGGG -+ -hhhccdcFcdddchhhhhhghhhhhghhhfhhhhhghhhhhhhfghhhgghhhhggghfhegfhgb]hdhhdhac]daWTTNEXWWTS_^`b^_abbdddcadcddRd_adbbcacaaadaaa^^\^`__a\\^^V^^\^^BBBBBBBBBBB -@HWI-EAS440_0386:1:24:12302:2593#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGGNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGTATTTGTGGTG -+ -aaa\\a_DRZV^Zcccc_cccccccccccccccccc]cccccccIcccZ]cRZ``VMZ^a]a]cccccbcccccaac_WJQLDVVTLK[[[[SLYTYYccccbccc`cZV[][PKSUW[ZXZ[X_BBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:8488:2754#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTTAAATTGCAGTTGGTACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATTGCGGTG -+ -]YZZZ_^DQZUSV^c]accccccccccccccccccccccccccccccccccccccYLcaccccc[POccccccaccQ[UMQGD^XZWTaaL]UIFUHT[]^]^NFFFSY\\XWH[ZJTcc]ccccbcBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:6114:3818#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATAGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG -+ -hhhddacF^bb`ahhhhhhhhhhhfghhhhhhhhhghhggghhhghghgghghgghhhhhhhghgffhhhhdccdafc]\\[H^\][[ccaedefcdfffdbfccdcdaabdbacda`_b^_a`aa]caa[]__Y[aBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:13084:4634#0/1 -TACGTATNGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGNGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGGGTGTCGGGGGGGGAAGGTGTATTCCTATGG -+ -hhhdaacFa[a^ahhhhhhhhhhhhghhhhhhhghghgcccffhghhgfghhgghhhhhhhggdaeffdhedebcdecZZZ^FZVYVY_^`Lb`^\\[daaaaddb_b^Q^]^X[^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:9705:4722#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGGAGTCGTGGGG -+ -ghheedeFacdacghhhhhfhhhhhahhhhhhhhhfhhgggghgghhhgegehhg_hcggehggfgbhfggfhcddhd]]^^H\^\\\dadebddddccfdefaadcdbabaadab]_]^WY^BBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:6985:4749#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGCTTCGGGGGG -+ -gggaa^aEZ```_fffffgagggggggggcgggggggggegggggfgggfgggggegggfggggdggfefeeade_gd[[ZYF\Z[ZZdd]dcgacgddcdcedgeadaacacWca_`ca_aaadd_aaa]aBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:2227:6089#0/1 -TACGTAGNGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCAAGCAACTCAGAAGTGAAAGGCTGGNGCTCAACCCCGGGACTGCTTTTGAAACTGTTTGACTAGAGTGCTGGAGAGGTAAGCGGAATTCCTAGTG -+ -gggdaacGdcccaggggggggggggdgggggggggfgfgegggggggggfgeggegggdfeddK]_^^bdeaddcfce\Z\`F\XX\ZcddadddQaaaaccaaaa___aba]b\_]_b_W]a_baaBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:7654:6279#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGAGTTGTGGGGG -+ -gggdeaeF^`b``geggegggggggfgggggggggfgggggffgfgggfbgdggdffgfgbfffcfbgdgdacaaada\][^G\[W[[a_[daaaabbccccd`cd`_a^`aaaa__W_a]]_^]___BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:11532:6376#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATGCCTTGAGTGCAGTTGAGGCAGGGGGGGTGTGGGGGG -+ -gggddccF`bbbaggggggggggggdgggcgggfggggggggggggggfefefgggcgggggggggggggggfecbfe]]]]F\[Z[Zddcfeaeddedbddedggcfeabcbdbeaa`c`\_JZSWZ]__BBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:6005:6667#0/1 -TACGTAGNTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGNGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGA -+ -hhhedeeHcdddchhhhhhghhhghhghhhhhhhchhhhhhhahghhhhhhehfhhhghhhhhhfhghhggegddghh`a`dFa][^^dgcdggbeegdaacdedcVa`]a^`cgWd]dd[d]ddb_a_][`PZ__^^Wa_\a_ZYaBBBBB -@HWI-EAS440_0386:1:24:9083:6728#0/1 -TACGGAANGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGANGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGTG -+ -gggdeedGdcddcggggggggggggdgggfgggfffffdffffdcfcfgggggggagfggggggcgggggddabeefd[Z[JFNRRRRcbddafaa_dcaaR[R^Y^Q\\\[^M\[[X^\^W\QRKUQaa_aaBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:9947:7672#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACCGGGTGTCTTGAGTACAGTAGAGGAAGGCGGGAGTGTGGGGG -+ -ggg\^\_DTWY_Wcfffcgagagffddffdfccffacfdddfdf_edffggfgbeabdeddadgdd`e]`b_b`\_a[OMMMEVRTNMaaaa]IRU]S___\_``b]_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:5510:8037#0/1 -TACGGAGNATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGNGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGTATGGTG -+ -hhhaaaaEdeedegghhhhhhhhhhghhhhhhhhhfhhggdhghghchgghfhfhhhhheheafafccbfdafddbgg^^]aG]\[\\ddacgecade`dddddbbab]_ddbdg[dddddWdad_ab]db__\^\\^\_]TZ\^X\X^_U_ -@HWI-EAS440_0386:1:24:5505:8369#0/1 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-hhhbb`bE\aaaaadfffhhhhhhhchhhhhhhdhhhhhgghhhghhhgehghhhghhghhhfhggfhghhfhdcbhc]\][H[ZYZXbbafddbbaddd_adade_d__cc]_aacYc\``_V\RS\\_^^^BBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:18470:10081#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG -+ -hhhddccFdeeeehhhhhhfhhhhhhhhhghhhghhhhhhfghgghhhdhfhchghhhhghghhhhhhghhgedcdhd\Z[ZGZZUYVecdhegfffgfgdchdggd_cbddabaa_bdaaabaddad\_c^_BBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:15353:10428#0/1 -GGACGCTNGACGCCATTCATAATGTTTTCCGTAAATTCAGCGCCTTCCATGATGAGACAGGCCGTTTGAATGTTGACGGGATNAACATAATAAGCAATGACGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAGGGTATCCTACAAAGTC -+ -gfgeeeeHedeedfffddfffffggggggggggggggggggggfggggggggggggfgggdgggfggegfggdggfdg]][]FNVSOVffgcdddfbdbcddccafadaabbbXa_aa]aa__aYb__Z]__HXSUSVHZXXO^_^^BBBBB -@HWI-EAS440_0386:1:24:5578:10837#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTCTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGAGTCTGTGGG -+ -hhheeaeF^``^`ghhhhhghghghdhhhdhhhhhghhcggggcchghffhe_ggggefgeffeddbgfffdgddagcZZZUFWSURSa_]caa__^_`_^_]^__X`_TaaaX[Y[I][ZP[S^UR]WWWMS^^Z^BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:8169:11098#0/1 -TACGTAGNTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGTTCGANGCTTAACTTCGGTAAGCCATGGAAACCGTAGAGCTAGAGTGTGTGAGAGGATCGTGGAATTACATGGGG -+ -fffaYaaEIQZ]Wffffffffffffcdffffffffcffffcfcfffbfbeffcffaacf_`c]facac]d`]`aaaca]U[]FZ[YXYddd_daO_a_a]a]_ZMPZ^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:15875:11235#0/1 -TACGGAGNATCCGAGCGATATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCNGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTTGGGGGG -+ 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-TACGTAGNGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGATGTGAAAGGCTGGNGCTTAACCCCAGGACTGCATTGGAAACTGTTTTTTTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG -+ -hhheadeHccccchfhhhhhhhhhhehhhhghhhhfhdhgghggghghfghghe_hfeghdddhaehhahedgdfffdPSLZEZRTZW_^`^_S\\KOQKNGN_^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:3831:14326#0/1 -TACGTAGNGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTAGGTGGTTTGTCGCGTCGTCTGTGAAATTCCGGNGCTTAACTTCGGGCGTGCAGGCGATACGGGTATAACTTGAGTGCTGTGGGGTAAACTGGATTTCCTGGG -+ -W[_L\ZLEJ_JMZa[a[e`ceda__e\__ed_``df_ffffca`cad]``]a\^aca^^a^_Pbb`a`dddddffffL]VZPD[\\\Zacd]dW__Y_ffffaSccaWdaadaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:24:12505:14386#0/1 -TACAGAGNTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGNGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGCGGAATGGCGGGGGAACCGGAATTCTCGGCG -+ 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-TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGAGGCAATGGGAATTCGGTGGG -+ -][^d`ddd[dadaffffffffffffdcffd[ffeedceedeeec_f`eacecf``UTTIPNPPd`ddd]ddadcaaY`]Q]M]ba]ba]aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:13850:1850#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTCCAGTGGAGGTGGGCGGGATGTGTGGGG -+ -hhheedeFaddccehhhhhhhhhhgghhhghhhhhfhhfgghhhfgghfghhdggfgfhfhffgdfdgdghdha_aa\dae[dcaaaaaa]daa______W^a_^a_aa_`aa_ab_Y^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:2639:1894#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGGGTGGGTGG -+ -gggffdfdfffffgggggggfggffbffgeggggggggdcaffgeggggegggggegggggdaebddceeecd\Z_b\bbb``aac__Y\Z^\aa_]cac\_caaa\cUF\ZZ`\^\XX\XMXMQKNQ\\\VX__U_BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:6139:2018#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGGATTCCTAGTG -+ -hhhhhhhhfhhhcfhhhehhghhhhhhhhhhhhhhdhefefffghhfhhhgaghgfhfhgh_df_dfeehedceab[edb_aJ_aaaaWQ\\^aa_[aa_aaH``_W\_^__T^[]\ZZQ^L^\`^BBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3109:2059#0/1 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGGATGGTG -+ -hhhhhhhhhhhhhhhhhhhhhhhhhahhhfhhhhhhhhhhehhhhhhhhhhhgfhhhhhhhdcfefdccfcafY__dbfdedagagegaa__ffaaacadbaafdbadbaedacdadaa__QaS^[\^Z`^^`_`J]^BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:6937:2060#0/1 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGTCGGGGGGGTAAGTGGAATTGCTAGTG -+ -aaacccccccccccccc_cccccccccccccccccccccc_ccccccccaccccc]cK\YM[\cabacccccccacaaccbcccQccc_c_cccaY_cBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:7669:2080#0/1 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGGATGTATGGTG -+ -hhhhhhhhhhhhgffhhghhhhhhhghhhghhhhhfhgghhhhgchhhgggchhhghhgcgfdgdgfcfgdcfcgddedgfKdcbcbe_daadba__aU]_`_dbaabaadd`]aaa__ca`a\_W__BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:7091:2092#0/1 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAAACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAAATGTAGGACTAGAGTGCAGGAGAGGGAAGTGGTATTCCCAGTG -+ -hhhhghhhhhhhghhhhghhghhhhgfhhhfhhhhdgdedefefhghhfhgaghgfgghhfffeaeheeddadddd]d_b____\Z_\\^\\_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3922:2110#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGGGGTG -+ -fffggggggggggggggggggggggggggggggfggggfggggfagggggggggeggggdggggdedgeffef``]b\ffcacdcddabaaddaa]ac_aa_caaaa\b]abba]daaW^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3806:2202#0/1 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATCCCCGGTGG -+ -gggggggggggggggggggggggggggggggggfafffdffff_ffcfggefggggggffedfafffRbd]d``afcaeedced]d]cba_deeab]dcaccJb``]]X^\XXcbb^`b`adaada[aac_a___aBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:6540:2228#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGTCGGAGGGGGAAGTGGAATTCCTAGTG -+ -hhhhhhhhghhhhhhhhhhhhhhghhhhhhhhhhhghhfhhfhhhhghhhhcfhaegcgadgghdcgfggheeffg_]ddbdafcdbcddddddcWa]___^^ddaIaVPZQ]V_^\\\_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:10019:2223#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG -+ -hhhhhhhhhhhhfhhhhhhghhhhhghhhhhhhhhhhhhhhhhhghfhhecghhhhhhhhhhghfgghghhdhedchchfhdgffddfddfefdaccedcd_eade_ca\aacaaa__``^R^BBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:2668:2254#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGAGTTCGCGGGG -+ -hhhhhhhhghhhghhhhhhhhhhhhahhhehhhhhghhggghheehhhedhehhhghhhhhgfhddbgdehchab]faeeeddgadfdaadecabaaeW`^^`aac\a__caa^^`ZZ]^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:19646:2248#0/1 -TACGGAGNATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG -+ -hhhee`eF^``^bghhhghghgghffhhhfhhhhhfhhfdfggddgehdag_hggdhgfggfdgddcgdddadaa`dacadaaaaaa`aa[caaa``aa]aaa_`___\[\Z_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:1802:2336#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGTTCTGTGGG -+ -hhhfdffffffffhhhhhhghghhhbhhhdhhhhhghggdghhhchehhgghhhhghhhhhffhdbehhhhcfa_]faccfaadagdaaaadaca_ac_a_acT^^T^^^`_`]b_d]_aa_aBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:5054:2329#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGGGGAATTTGGGGTG -+ -hhhhhhhghhhhhhhhhghhhhhfhehhhhhhhhhghhhgghggehhhfhhhdfheghhfghhhfgfhfhfghdcae`dggedffcfd[baebfcdffffdfgddbd]bab_aca_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8482:2380#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGGATTCGGGGTG -+ -gggggggggggggggggggggggggcgggggggfgggggggggfefffeffdffffffgggggfgggggggggdddfbgggfffgggagfg]ddcacdgggceaef][^^b`bWcc`acddadca_ada_a]aBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:15658:2382#0/1 -TACGGAGNGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGGTGCGTAGGTGGATTATCAAGTCTCTTGTGAAATCTAATAGCTTAACTATTAATTTGCTTGGGAAATTGATAGTTTAGAGGAGGGGGGGGGCAGATGTAACTCTTGGGG -+ -`cc\_a\DTQY[Vcccccc_ccccccccccccccccccccccYcU_ZcccccWccYccccWcccccc[\Va_accc_aUNRWPccccccccacBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8261:2388#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGAGTGTGTGTG -+ -hhhhhhhhhhhhfhhhhhhhhhhghdhhhghhhfhhhhhhghgehhhhhchgghffhhhhgaghehehhhgghdd_fafcccc_dgddddbadddcbeabaaeddh]_a_cacaddaX^^^K^[`]_`\`\[\ZIZ^BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:9735:2401#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGGTGTGTGGG -+ -hhhhhhhhhhhhfhhhhhhchhhhhghhhghhhhhghhgghhhhghhhggegfhgghffhdgchbcdhdhhdhaaac_eaddadddeccadedaaaadaaaac^aaWW[Y[X^a_a___`^R___X^_BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8948:2437#0/1 -TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTG -+ -ggggggggc`fffgggggggggggggggggggggfggggggg_ggggfggefgggggggeggggfgggcggfgffg[fd_cdadedegfffaf_aadcaca_cdaeddfdd]fab]cdbdca]U]]]`U]]ZS]\UY\X\aY_BBBBBBBBB -@HWI-EAS440_0386:1:25:12681:2438#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGTGGTG -+ -fffgggggggggggggggggggggggggggggggggggggggggggggggggdgggggggegggdgdgggggggaggdgfgggeffffaddgdfbafcbfdffdffdfdbbdb_dd]a_`\\_VKW\]ZP\X`]]]Z`BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:12916:2459#0/1 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGTATGGTG -+ -hhhhhhhhhhfhgghhhhhfhhhhhehhhghhhhhghhhghhfhfhhhghhehhhfhhhcgfchehedgheagaceeedhdWacdccddeccgdbb_d`abdda`cRc_abbbde]baaabWb^a_\__[[]aa\__aBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:7336:2484#0/1 -TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGCGGAATTCGTGGGG -+ -fffgggggfdfffgggggggggggggggggfgggggaggggggfbfafddfddffcaa`cafcecddfbfedeebcaacadbbdegddddYgaZ`[YY\^]\Zddd`d_]`b]KYMTQ^ZYTQTTYKV_RYSY_BBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:1489:2530#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAATCTGGAGTTCGGACAAGGAAGATGGAATTGGTGGGGT -+ -fffffffffffffaffffgggggggegggggggggggggggggggggggggcggggg]fdfdfgggagafddf`b`bb]W]\\caa_Q^__b_^W[`R^^\^\edabd_aaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:9578:2531#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG -+ -hhhfhhhhhhhhfhhhhhhfhfhhghhhehghhhhgghhhhhdhdhghccff_ffafffghhhhfhehfhhggdddfagdggeefgffffdfcdadcd`bccb`aacca___adadabbacaaaacdd^a_]aBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:9159:2552#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGAATTCGTGGGG -+ -fffgggggagggggggggggggggggggggfgggggfgggggafgggggfggaffdfdcfaffggg]gcafcfggfgdc]fa_fdfdfgeagdc_acccgec_b]ebdedgaa]_^Y]^^WWQU\WNYZ[[Y^]H]`BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:11396:2559#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGGG -+ -hhhhhhhhhhhhagghhghghhhhhbhhhehhhhhghhhgfggedhghdehhahgeghhghhfgdgfgfggfgcaahbgdegdffcfddbfgdfcd]ffdcdfcccaddadd_aadaacfcb\`aaaa^\\LY\\^BBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:1760:2573#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGTCAGGCGGAATTCGTGGGG -+ -fffffdfffdfffaffffgdggggcgggggcggcffffggcggfdfdff[fdfffadfdffd]ddfdfcdeef[\`d[YW^W[beadcdgbed]]Z``ccca___caac]_`aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:14068:2621#0/1 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG -+ -ghhhhhhhhhhhhhhhhhhhhhhhhhchhhRhhfffffhhhhh_ghhfhhgfhhhhhghhghhhghhcdffdffgghghghhfcehae[caccahcgghhfffehedg``bd`dhchXhhebeeddde[aa[c\\^^^[_BBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8159:2639#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTGGGGGTG -+ -hhhhhhhhhhhhhhhhhghfhhhhhfhhhghhhhhfhgggggghghghcdhfbhegghgdhgdgdgdhdhgdhacaeaebcccfdfddcccfbacaac`adadcfcd`dababaadaa_daVaa_aaaU^BBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:13134:2667#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGCGCGGCTGGCGGGCAGGAGAGGTACGTGGAATTCCTCGTG -+ -hhhhhhhhhhdhfhhhhhhhhhhghhhhhhhhhfhdhghhhhhgghhhhhhgfggggheffddhaghhadgdefffafdfdfafcdacd`aadaa\ZRRYTMY__^U^\O\^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:11175:2690#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGGGGGG -+ -bbbfafffdfffffffdffffffffcfffffdcffeffffffffeccfeffdfffadfdfcfffef]feffefeddfdede_ddddedadcecbc_aadcd]dcbeadcade]Z`]Y]``W]]Z``BBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8576:2709#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATGGTGGGG -+ -hhhhhhhhhhhhehghhhhfhhhhhehhhghhhhhhhhhhchghhhghfhhghhhhhhhhegghfgfhghfdcaaafafgegfbfgfdaddhddaddbddgbgadeaaa_aaab]d_a^__^_\__aaZ]_^]_^]`BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:6943:2731#0/1 -TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGGG -+ -fffgggggggggggggggggggggggggggggegfggggggggggggggggggggfgggfggegggggfggdgcddcd_bddacegddfagafb_dfbddddbfdffaaddad``da```dWda``]bT\_[]__BBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:1850:2741#0/1 -AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCGGAATTCACGGTG -+ -cffdaddd^aKa^fffffffffffffffff]ffcfffffffffffffffffffff`]]f_]f[d^add_ffcffcfacfda]ccfcfeefcWeaa^^]fcfca`_`cZ```dT^aZZS`ccaaa_a_cPM^W^^LWYYZ^BBBBBBBBBBBB -@HWI-EAS440_0386:1:25:10428:2744#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTCGGTGTG -+ -hhhhhhhhhhhhggghhhhhhhhghahhhehhhhhghhhhhhdehhehdgegghhehhhhghghdhghghhehbdbhagghedgcdfdbaagdcbacebddagaadb]a\aaabdac`_aa]a]aa_ba\^_BBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:12398:2774#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG -+ -hhhhhhhhghhhchhhhhhhhhhhhehhhghhhhhghhhhghhhhhhhhhhhhhdhhhhhhhfhggdhghgghbdghgghh_dgdgedfcfgfdeddeffadgadd[e__acccbacaccaaa]aaadada__]_\_aBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:14110:2805#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGCGTGTCGGAGGGGTAAGTGGAATTCCTAGTG -+ -hhhhghghdfffdhhhhhhhhhhhhfhhhghhhdfafccccfffhhhadghhegghhehhfcab_]dbR_^]_fdff__cac_dWbb\aacad`bKRW\\YZ\_^KR\XL[Z[^`^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:14127:2819#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTCGGGTGG -+ -ffffffffgggggggggggggggfgggggggggfgggggggggfdggggggggggfggggggfdaddfdffefbbaf_ddecdddfcddaagdcdaddffadfa`dac_`gcb^bd_^acaY]UW[\\\aBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:1968:2841#0/1 -TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCTAGAGTGTGTGAGAGGATCGTGGAATTCGATGTGT -+ -ggggggggdffffggggggggggggegggggggcaffeggggaffcccggggffggaefebffeadffcfc]c]X`b`bdddaddaabca_de]caa_bRI\_``_\_a`ddabba_bBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:10866:2839#0/1 -TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGAACCGGAAGTCTCGGTG -+ -hhhhhhhhhhhhhfhhhhhhghhhhhhghghhhhgfhhhhhhdhghhhgghhhhhhhdhhegdfcdfahehcefffafcfddbd__dda^d[]^_`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:11767:2838#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACCGTAGAGGTGGGGGGGATTCGGGGGG -+ -hhhhhhhhehhhhhhhhhhhghhhgghhhghhghhghhheghheghhhgeghhcgghghhhhghdgchfhgfeccbeba_cdaedebeb]dccaaaaa```^`_aa_d_^]L_`_a^^_caL`BBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4402:2852#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTGTCTTGGGGACAGGAGGGGTGGGGGGGAATTGTGGGG -+ -hhhhhhhhhhhhhhhhhhhhhhhhhghhhhhhhhhhhhhhghhfghhhegghghfhhhhghhhhfgghghfghdbbfbeeheegcefa\__caaaaaaca^_daaa\a]\a_aaaaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:1421:2878#0/1 -TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGGCGGGACATGATACTTCGAGACTAGAGTAATGGGGGGGGGACCGGGGTTTTCGGGT -+ -_ffK[b]`fffffggggggggfggfgggggggggggggggggffffffgggggggggggggggffffffffdf[ccfaafafd`Q]b_cKcccBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:11319:2883#0/1 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG -+ -ghhhhhhhhhhhhchhhghhhhhhhahhhfhhhhhfhfhghehgghhhfghhhegghfcehddhdehceggfdfagbegfdfcfbcdabbaaddb]a_a]aW_aaaYaZ^^^X_a]b_]Z^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3648:2910#0/1 -TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCGCATGATACTGCGAGACTAGGGTAATGGAGGGGGCACCGGAATTCTGGGGG -+ -hhhaffffhhhhhhhhhehhhghhhhhhhhhhhhchhhfffffhhhghhhhhhhhhhhhhhgefdffdfdfefeebaedgggagcadbdbgddaacVa^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:6586:2930#0/1 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGGAAGCGGAAGTCCGAGGG -+ -hhhhhhhhhghhhghhhhhhhhhhhhhhhhhhhhhhhghggghhghghdhhedgghhhhhhhghfhghacgdgfgggfggdfcabce]ccbaed_^aa_a_VWaaZ_\__^_\Z`\^^^^_Y]\_\aBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8471:2931#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAGTTGAGGCAGGGGGGGTTTGTGGGG -+ -hhhhhhhhhhhhhhhhhhhhhhhhhfhhhghhhhhghhggghhhghhhddhehhheghhhggdhfffhfhhfhffchdffgfgcdd`abbdcab_aadaacaeddf`aa_abb[[^FJ\][[ZY`O^`BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:14208:2940#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGGG -+ -hhhhfhhhhhhhhffhhhhhhhhhhhhhhhhhhchhhhhhhhghggghhhgehhgghfhhhhgfdhcfehhghgedhdgfhfgffdf_ddafddacdddcfdggcfggfccbfacaa^cac_a^^`W`^_c[aVL^BBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:2027:2951#0/1 -TACGGAGGATCCGAGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAACTCGTGGTG -+ -fffffffffffffcfcffgggggggaggggggggggggggggggfgggddf_cgggggcggggfdgaggegdgdc[fceefbeddbdd]a_fadaaaadd]be]```BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:13175:2941#0/1 -TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATAGCAAGTCAGATGTGAAAACTATGGGCTCAACCTGTAGATTGCATTTGAAACTGTTGTTCTTGAGTGAAGTAGAGGTAAGCGGAATTCCTAGTG -+ -hhhhhhhhhhhhhhhhhhhhhhhghhchhhhhhfhehehhhhhhhhhhghhhhhhhhgghhhghfhghggfghgffgcggggbefdffdabgeadeacddcaeddddaaaedadd_d_caa_aX\ZZ\^_`\`_\^``Za]WZW[\R\\_BB -@HWI-EAS440_0386:1:25:13489:2951#0/1 -TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGGTACTGCGAGACTAGGGTAATGGAGGGGGAACCGGGATTTTCGGGG -+ -hhhhhhhhhhhhhghhhhhhhhhghhhhghhhhghfhhhhhhchhhhhhddfhhghhhhhhgffdffdfcdbdggdgbKUW\\`ZU__aaaaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:6132:2959#0/1 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGGAATTACTAGTG -+ -`]afffffffcffffffff_fffffffffffffffffffffffcffcfbee]^ffffffdffffffWffffcffaf[fdf[ffdcdffffddfP\^^adec]bd]bdbcdadb^]KJ^`\^^\\a_aa`[]]N\X^W^]]^BBBBBBBBBBB -@HWI-EAS440_0386:1:25:2692:2971#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG -+ -hhhhhhhhhhhhhehhhhhfhhhhhahhhehhhfhehhhgggghghhheghfdhhgghgghg]hehdhggfghab]fbedhddgfdfcddcfcabaaaccddg_[aaadabaddeddaddd_`Y^_aa[TWFQ[[[UXZ^`BBBBBBBBBBB -@HWI-EAS440_0386:1:25:1831:2983#0/1 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAGTGCCGGAGAGGTAAGCGGAATTTCTAGGG -+ -deeffffffacffggggggggggggfggggfgggggggggggggggfgafdffdf_ffffdeefdbffbbd`bcccWfZ_`___accaedgdca]Q_`dda]a``]]X]\``VV]]_^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:6335:2989#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAGTAGAGGGGGGTGGAATTCGTGGGG -+ -addfffffRdadcdddddffR[fddc[dff_dcffa[fdddd`aMSaaT]_]Kdd[ddffffcfefff]ff`ca^^[PUNSTXcecfeeecaPTIXYN`````G^^ZZebbaccc_]aacaaaWT]Z`BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:10162:3028#0/1 -TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCGGAGAGGGTGGCGGAATTCCAGGTG -+ -aaafffffccfff`b`_KZ[TWZfcffffffeffaffdffffffffdfdfdfafdfffdfWddededfeae`cbbdacaeadddd`daaacc[]`W]]`ddad_caaWca_aO]XH]]_\W_]YRYW_BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4348:3042#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGTGTGGGGGG -+ -[_affffffffffffffffffffffcfffffddfffffffffffffffffdffffffadfffffdffffffffafQfafcffcdfcfedfefdfeecddfcdfdecdfccacebWebaccbbccaeabT``]`]`[``BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:5972:3048#0/1 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAGAGTGCAGGAGAGGTAAGTGGAATTCCTAGTG -+ -hhhhhghhhhhhhhhhhhhhfhhhhhghhhh_hhhchhhhhhhdghghghhghhhhheghhgfhfgdhhheghfgfcgggbfdbbcdfgcdcfdaaadcfcafbbb^`_\a__Wa_aBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:9861:3050#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGGTTGTGGGG -+ -fffggggggggggggggggggggggggggggggggggggggggggggggggggfggggggggfggggggggdgbc_fcgaggggdffdd`bd_dbdbbaca[c\c__`]W````T_WX^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:10090:3050#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGATTGTGGGG -+ -hhhhhhhhfhhhgghhhhhhhhhhhdhhhdhhhhhghhgggggggghhfgdghhhghgeghbfgdedhdhgdg_\ac_cdedbaYaaababebaab\bbb_Wd_baabaaaaacWc_Z``_^^accdfca``c\]___BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3139:3077#0/1 -TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTGGAGCGGCAATCGGAATTCCGTGGG -+ -ggggggggcffffgggggggggggggggggfgggggggggggggfgggedggggdgggggggggggcggggffgfgfcgggefffggggggegfgfgfbddQda[`c_dddaadf`fdbdaaWP^Z^^BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:9110:3081#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGTGGTTGGGGGG -+ -hhhhhhhhhhhhhfhhhhhhhhghfghhhghhhhhghhfghhhhghhhghhfhhhghghhhgghfhghghhfhcbahdeggegcfgfdcadfbdcbcdbaaad_cc_a`]`^ba_a__acaaa\a_\aaaaaacVaaaBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:17734:3100#0/1 -TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGT -+ -ffffffffcffffggggggeggggggggggggggggggggggggfgdggggggggdgedeaffgffggadfdfegadggagfggcgggfgfagbW]W^dbcddddb[abdda_XPZ\\aaYa_aa]a_NYYYZT\\]]`BBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8423:3141#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGCGGATTTGTGGTG -+ -hhhhhhhhhhhhhhhhhhhghhhhgghhhhhhhhhghhhhefggghfhfgghhhhhhghhgghhfeehgggfhbgfgfgffgdcggfceffffffca_abbac`ad_d_aaaaddae_^`]^\\a_N\^_^]Z_aa_BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:14009:3149#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGGGGAATTCGTGGTG -+ -hhhhhhhhhhhhhfghhhhchhhhhfhhhehhhfhhhhhhhhbffhheacff_heehegfghgcadbdgfgfeecWebfbffffefdacdddaddfdfaa]aa_aabb`\Z^^\[^ZS]`_\^Y]_^^Y__G_BBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3100:3158#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGGGGGGGAGTGGGGGG -+ -hhhhhhhhhhhhhhhhhhhchhhhhgfhhhhhhhhfhhhehghhghhhfhgehhghhghfgffgdg[gfgfdeab]dadddebfdfcaddbdaabb[_acaadcacac_ababccc`ca_\\_^_a\__aRa\__BBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4738:3152#0/1 -TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGGATAATGGAGGGGGAACCGGAAGTCTCGGTG -+ -gggaffffdgggggggggggggggfgggggggggggggffffffffffgcfggggggggggggfdffdfdfdfgcg]ggfbeffaccddabdb`Qb]_[^\]`a__]b_a]_bBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4197:3165#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGCGGAAGTCGTGGTG -+ -hhhhhhghhhhhgfhhhhhhhhhhgchhhhhhhhhhhhghhhfhhghhhhghhfff`ffcffchhhhhfhhhhfhhhbhggheghhhhchfhf_adddaeedeeeeadgddffcccf\aaaaa`gcda`_`\W^X\R^BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:9072:3171#0/1 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGTAGGGGG -+ -ggggggggggcgeggggggggggggfgggfggggggggggggggggggffff]gggggdcfccfefffcffafddfaffWf_cdffdeded[ffddedffgfRcfaaaccfc_fecddcacaa]aaacaac_]^YZ^^\^aBBBBBBBBBBB -@HWI-EAS440_0386:1:25:15751:3166#0/1 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGGATTCACGGTG -+ -gggffffffffffgggggggggggggggggggggggggggggggcggggggggffPc[^`Z\Zfefaff[fcfccffa``cf_ggagefgdfd[^\WWccc`c__aaQYZ^_^a]]cZ`X`WbBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3473:3225#0/1 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGGAATGTATGGGG -+ -gggg_ggggggggggggggfggggggggggggggggggggggggfgggfggggggggggggfdgggegdfdcfcccffeeecc`adddccbefddcab`daddddd_daadaaddddWa__Y[W\\[\a``]`_Y_W_BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4755:3229#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGCGGTG -+ -hhhhhhhhhhhhhhghhhhhhhhhhghhhhhhhhhhhghgehhghhghggghhhgghgghhhghfhgheghegdcahceedggffggdedahdeebddc]dcdaddcaddgcadedacfddccaedcdabaaaZbaaaBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3032:3237#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAGGTAGGCGGCATTATGGGTG -+ -hhhhhhhhhhhhhghhhhhhhhhhhghhhehhhghghhgghgghhhhhgehhfhec`fhhgeggdg_hghhfhgffhdgfgdgfgegfdddhggddd_ddddhdegadcefceffddcddaaPW`___a_WW_BBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:1630:3249#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGGGGTAAGTGGAATTTCTTGCG -+ -hhhhhhhhhhhhhhhhhhhhhhhhhghhhhhhhhhhhghfhhfhhhhfhhhfhhhghhhhhggghhhghhhghggh[gehegdhgdgffgbagaf[ccc`^_`c_W\a``^[U^``^^P[[XZQTSU[^JY_BBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4476:3251#0/1 -TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGCACCGGAACTCTCGGTG -+ -hhh]ffffhhdhhhhhhhhghhhhhhhhhhhhhhahhhhhhhhhefhhgghhhghhghhfeggfdffcfbfdfdfeddgeggf_H_^^`Z`\ZYZ[I\Y\[\^^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3312:3259#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGAATTTGGGGGG -+ -hhhhhhhYcffffefhhhhghhhhgehhhchhhhhghhhgchhhbhhhdghghhhghhhghhgedd]hhggfhfd]edddegddffffbgdgffdfcddff_fccdcfdadfcad[dadaaab___BBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3550:3291#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGGG -+ -hhhhhhhhhghhchhhhhhdhhhhgeghhhghhhhgehhhghhhhhhhdgfhchhchfheeahhgdchdddefdgdhaebecdfgfffgfgfd]ddcdadaaccbbccb]cbb_b^babd_aaaa]X_[]^Z_JZ[^BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:5478:3298#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTGGAGGTGGGGTGGAATTGGGGGG -+ -hhhhhhhhhhhhhhhhhhhhhhhhgghhhghhhhhghhhhhgggghhhgghhfhghhhgghhhgbhghegheedd_gcgfgefffffdfddgdfgdbfddffgaaccaaWa_a__`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:15346:3296#0/1 -TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGGTTCGTGGTG -+ -hhhhhhhhhhhhghhhhhhhhhQffcffhghhhhghhhhghghhdhhhhehhhhhghhhhhhfheechhhhghddchdhdeggadhbdacbdadbcdddgdaeabdaca\aac^``^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:3468:3322#0/1 -TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGGGTGGAATTCCGCGTG -+ -hhhhghhhfffffhghhhhgahhhghhhhhhhhhfhhgggghhhfhhchghhhhgghedhdddecbeefdfcgffdffffgfddcffddccfeWdaabcddcaad___ababaa_daaaa_\\S_^^R\\\XX_Pa_BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4199:3321#0/1 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGGAATTCCTAGTG -+ -hhhhhhhhhfhhhfhhhhhhhhhhgchhhhhhhhhghhffdffhghhhhhghdhhhdhhhgfhhghhfhfhdhgghgggfdfdb[dgbffgded[da_aad]bbda]aZN\^\ababa]aa]__a[_^[WTIXQWTVW\_aBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8568:3330#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGGGGGATTCGTGGGG -+ -fffgfgggggggfggggggegggggeggggggggggggggggggdgggddfcfgggggggggggfggggggggcffgfggggedgffagadgdfgfgffgfcg`dgdgaacaaaab]adddaa`d``ddad`a][_aBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:12050:3335#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGAGTTGTGGGG -+ -ggggggggdggggggggggfgggggggggcggggggggggggggggggggcggggdeggggg_gggggdggdfdddgcgdegdfgd_dgffgfb_aWcdddbdaddcWZ^\^^cabcabbbacaa[aaabdWdR\R\^BBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:19075:3342#0/1 -TACGTAGNGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGGACTGCATTGGAAACTGTCGTACTTGAGTATCGGAGAGGTAAGTGGAATTCCTGGTG -+ -hhhdeeeHdcccdhhhhhhhhhhhhhhhhhhhhhhhhhhhhhghhhghhfhghhhhhhfhhddhbefhdfhcfdeaedaebedd__baa]bab_a[G`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4994:3354#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGGCGGGATTTGTGGTG -+ -fffgggggggggggggggggggggggggggggggggggggggfeggggffgeggggggggggggfgfgdggdgc]bdbffgg_ffbeffdfgfa_aaaddddg_aabba_a]bd[b_aadaa`cd``]\`__\Y\\^BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:12091:3381#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGAATTCCTAGTG -+ -dddffffffffffffffffffffffffffffffffffffffffffffffffcffffffffffefeffffcfeffe]dfdfdedcWcfcdddafcfacecacacdd[adc__`_WaZ\X]W]J]ZZSXZ^BBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8594:3395#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGTG -+ -hhhhhghchhhhhchhhhhhhhhhfchhhdhhhhhhhheeehhhghhhgdhhhhfehhhfhhghdgdhechehfffgdhdcgbfgfggcddhddddddfff]fcdcaa]`c`c_`Z]S[\[XUVY[[\Y^BBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:2052:3400#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGACTAGAGGGCAGGAGAGGTAAGTGGCATTCCTCGGG -+ -ggggggggggggggcgggggggggggfggggggggdggffafffggggggggggfggggggfdfeefeefecedegecgddgabQadbW\^^`aaa_c__]aaaa_T_VV\\Pbb_aaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:11776:3405#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGAATTTGTGGGG -+ -fffhhhhhhhhhhhghhhhfhhhhhchhhfhhhhhhhghhhfhhfhhgdfffbhhehhhghhhhgfghgghdggdccgeahceeggedfffgf]ddadabdab_aaaa\UX\KYPWUYdadabZZ_Y_BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4155:3413#0/1 -TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGACGGCACATGATACTGCGAGACTAGAGTAATGGAGGGGGGACCGGAATTCTCGGTG -+ -gfgaedeeeffffcffffggggggggggggggggfgggbbbdffffffgggggggeggggggffcffafcc_fedddbbfgbfaO___\W^^[QW^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:11052:3431#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGGGGTG -+ -hhhhhhhhhhhhfhhhhghhhhhhgghhhhhhhhhghhgghhhhghhhgfgghhhgghghhhghdddhghhfhebchaggh_ggfdcc]a_cadaaadddadebdfdda[caaddada_aa[b\aV_a_ba_BBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:4122:3435#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTAGAGTGCCGGAGGGGTAAGTGGAATTCCTAGGG -+ -S[_]cccaccccccccccccccccccccccaccccccccccccccccccccccccc[accccccccccccccccccc[ccc]cc[cbcccc[cSWR[W]][]SaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:9796:3444#0/1 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG -+ -hhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhghghhfhhfhhghhhhhhgghahhhhhhhfhdhgfhhhdghh_hfhgecghfchggeddhfaa[cceeeedecdaacffaebddbccc_c_eaaccZaa``_cXcYY^R\[UTWTTZY[ -@HWI-EAS440_0386:1:25:2235:3462#0/1 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTG -+ -gggfdfffefaffggggggebggggggggggggggcgdeeef_ddde[geggggcgeWcgaggg`fgfeec`cccebaddcc`bdadadaV]d]]H]_dcbb_a[]YTYUY]UW]O`ZX]ZY_^[]^^_aBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:14529:3458#0/1 -TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGTGTGAAAGGCGCGGGCTCAACCCGCGGCCGGCACATGATACTGCGAGACTAGGGGAATGGCGGGGGAACCGGAATTCTCGGTG -+ -hhhhhhhhghhhghhhhhhegfhhfhfhahghhhchhdhhhhahhhhhggchffgggghhggfhbefbfbd_dddcdaR`_`a_NU^^^^__^`^`G^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:6708:3509#0/1 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTG -+ -fffgggggggfggggggggfgggggfggggggggggggggggfgggggggggggfagggggagggggggegfgaggefdfddadgggccca_fa\^\^ddd]dacR\_``accc]aab`[`\`BBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:13919:3507#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGGGATTCGTGGGG -+ -hhhhhghhehhhffghhghchghhfahhhdhhhggdhhgdfgggdgfhcadfahedeeddgdagdebgffgdgd_agaeceddfdccdaaagd_caacadaadcddcad]`dbcbca_aa_Yb_aa_a\O\O]^\]_BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:10705:3535#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAACTGGCCGGCTAGGGTGTCCGGGGGGGACAGGGGATTTCCTGTGT -+ -hhhhhhhhdhhhhhhhhhhhhhhhhehhhhhhhffdfeffffchhhhhhghhhhhhhhfhhhfhdhchhfheagghhcahgeg_ddbadeedh`][^`Z_ZZ```RK^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:7788:3570#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG -+ -hhhhhhhghhhhghhhhhhhhhhhhhhhhhhhhghhhhhhghehhhhhgghehhhgfgghghhhgghheehbhaWacfc_ca`bedcfbddaeeebaaadada``ZW^]^```aabb_aa]]b_ca_a_V___WRZW_WX[VJ\W\G\T^X^ -@HWI-EAS440_0386:1:25:2273:3577#0/1 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGACTTCCCGGTG -+ -cfffeffeggaggffffdggdgggggaggggggee]fb`]```fcfgdgaffgffbbd[ca_W]Z``ZY^]U]YS[^`_`_`\`add```aa]a_[[a`a_a```a`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:14850:3657#0/1 -TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTGTGGTTGAGGTAGGCGGAATGCGTGGTG -+ -[__fffffffaffffffffffffffffffffcdfffffffffffaffffffcfffa[affffffffff][fffcfcfffdfcfffffdfed[cfccccfffafccfcYaaccc^^Y[^ffcaaZaBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:11581:3662#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGGGTAAGCGGAATTCCTAGGG -+ -hhhfhhhhfghhffhhhhhghhhhghghhhghhhhdhdheefffhehghhgehghgcehhhfdgdgghghffedff[gdfac]_]`c`ddd_c^`\[\_a___a_a]aaaab`__H]_[S]P]__]Z]`SY_`BBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:16965:3660#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGATTTGTGGGG -+ -hhhhhhhhhhhhhhhhhhhghhhhhchhhhhhhhhghhghhhhhghhhgchehhhhhhhhhhghfhfhgghdheedhdhghehffgfgcgggfgfa[ddcgdeffgafdcgggabadaddc_aW___`_a___W^R^BBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:8197:3691#0/1 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCGGAGGGGTAAGTGGAATTCCTAGCG -+ -hhhhhhhhhhhhghhhhhhhhhhhgfhhhghhhhhhhhhgghhhhhfehhhhhhhhhhhhhgfheefhehhehfgahgfhghfgffdccacaec]a__aaa\aaca^_aYaaadddba^^`S_S^\\^BBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:11617:3701#0/1 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGGCGGAATTCGTGGTG -+ -\a_fcfffdfdffffffffdfffffcffffdddfffafffffffcfffffff_ddaad^^^cafffdfaffcffffab^O^[aefe]acfccfbaccccccccdffeccc`fJGQYRXca```BBBBBBBBBBBBBBBBBBBBBBBBBBBBB -@HWI-EAS440_0386:1:25:15484:3705#0/1 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGGTAGGCGGAATGGATGGTG -+ -hhhhhhhhghhhhhhhhhhfhhhheehhhehhhhhghhhgdhhhghhhgghehhhhghhhggghfggeghgdhcaaefgegedgdhhdacaddcbcde\caccbac\c_acdacebb]a_aSa\_\]_\aa]W^BBBBBBBBBBBBBBBBBB diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/histograms.tabular --- a/test-data/split_libraries_fastq/histograms.tabular Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,8 +0,0 @@ -Length Count -116.0 20 -126.0 31 -136.0 33 -146.0 2 -156.0 0 --- - diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/histograms.txt --- a/test-data/split_libraries_fastq/histograms.txt Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,8 +0,0 @@ -Length Count -116.0 20 -126.0 31 -136.0 33 -146.0 2 -156.0 0 --- - diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/lane1_barcode.fastq.gz Binary file test-data/split_libraries_fastq/lane1_barcode.fastq.gz has changed diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/lane1_read1.fastq.gz Binary file test-data/split_libraries_fastq/lane1_read1.fastq.gz has changed diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/lane2_barcode.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq/lane2_barcode.fastq Sat Aug 05 07:27:17 2017 -0400 @@ -0,0 +1,1000 @@ +@M00176:17:000000000-A0CNA:1:1:15487:1773 1:N:0:0 +TTAGGCATCTCG ++ +B@@FFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:17088:1773 1:N:0:0 +CACTCAACAGAC ++ +C@@FFFEFBFHH +@M00176:17:000000000-A0CNA:1:1:16738:1773 1:N:0:0 +CTCGCTTCACTT ++ +B@@DFFFF:B+A+? +@M00176:17:000000000-A0CNA:1:1:14327:1796 1:N:0:0 +CCAGTAGCTTAA ++ +?@=DDADBFHFB +@M00176:17:000000000-A0CNA:1:1:14533:1797 1:N:0:0 +AGTTGGCCGAGT ++ +?;@DDFFBD:D< +@M00176:17:000000000-A0CNA:1:1:18569:1797 1:N:0:0 +TTAGGCATCTCG ++ +BB@FFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:18992:1797 1:N:0:0 +TTAGGCATCTCG ++ +B@@FFFFFHHHF +@M00176:17:000000000-A0CNA:1:1:14919:1798 1:N:0:0 +TTAGGCATCTCG ++ +=@<1:AB=ADHD +@M00176:17:000000000-A0CNA:1:1:18955:1798 1:N:0:0 +TGGTAACGTATC ++ +-0((2)2(.<)2 +@M00176:17:000000000-A0CNA:1:1:14684:1798 1:N:0:0 +TTAGGCATCTCG ++ +@@@FFFFFHGHF +@M00176:17:000000000-A0CNA:1:1:16933:1798 1:N:0:0 +TTAGGCATCTCG ++ +BBCFFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:15580:1799 1:N:0:0 +ATCATCTGGGTT ++ +@BCFFEEDHACF +@M00176:17:000000000-A0CNA:1:1:14654:1799 1:N:0:0 +TTAGGCATCTCG ++ +BB@DFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:17702:1799 1:N:0:0 +TTAGGCATCTCG ++ +B@CFFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:14715:1799 1:N:0:0 +GCATTGCGTGAG ++ +BB=DFFFFFGHD +@M00176:17:000000000-A0CNA:1:1:17454:1800 1:N:0:0 +TTAGGCATCTCG ++ +B@BDDFFFHHHG +@M00176:17:000000000-A0CNA:1:1:12800:1800 1:N:0:0 +GAGAGGGGAAAG ++ +0((((((((((( +@M00176:17:000000000-A0CNA:1:1:16784:1800 1:N:0:0 +GACTAACGTCAC ++ +?8?D=DFFFHFH +@M00176:17:000000000-A0CNA:1:1:17909:1801 1:N:0:0 +TTAGGCATCTCG ++ +BB@DFFFFDFHF +@M00176:17:000000000-A0CNA:1:1:15637:1801 1:N:0:0 +TTAGGCATCTCG ++ +BB@FFFFFHHHD +@M00176:17:000000000-A0CNA:1:1:14448:1801 1:N:0:0 +TTAGGCATCTCG ++ +::==A:=BDD?> +@M00176:17:000000000-A0CNA:1:1:12483:1802 1:N:0:0 +TCAGGTTGCGGA ++ +B@+ADEFF?HDC +@M00176:17:000000000-A0CNA:1:1:12930:1803 1:N:0:0 +TAAATCAGCCTC ++ +?@1DD:BDBFHF +@M00176:17:000000000-A0CNA:1:1:14068:1803 1:N:0:0 +TTAGGCATCTCG ++ +BB +@M00176:17:000000000-A0CNA:1:1:13982:1816 1:N:0:0 +TTAGCGTCACGA ++ +B@;ADDFFFHHB +@M00176:17:000000000-A0CNA:1:1:14848:1816 1:N:0:0 +CGAGCAGCACAT ++ +B@=BD?:BBCAF +@M00176:17:000000000-A0CNA:1:1:15011:1817 1:N:0:0 +TTAGGCATCTCG ++ +B?@FDFFFHHGH +@M00176:17:000000000-A0CNA:1:1:18535:1817 1:N:0:0 +TACGACTTTAGC ++ +B@BDBDDFHHHH +@M00176:17:000000000-A0CNA:1:1:17933:1817 1:N:0:0 +CTATAGTCGTGT ++ +BB=DDFFFHCFF +@M00176:17:000000000-A0CNA:1:1:18442:1817 1:N:0:0 +TTGGGCATCTCG ++ +B?+=DEEAFHHH +@M00176:17:000000000-A0CNA:1:1:18207:1817 1:N:0:0 +TGCACACGTATC ++ +11++2<7AA?7A +@M00176:17:000000000-A0CNA:1:1:13068:1817 1:N:0:0 +TTAGGCATCTCG ++ +B@BDDFFFHHFF +@M00176:17:000000000-A0CNA:1:1:17613:1818 1:N:0:0 +GCACTCGTTAGA ++ +BB@DFDADFHHH +@M00176:17:000000000-A0CNA:1:1:16588:1818 1:N:0:0 +ATGGGAATGGGA ++ +=:=DDADD?3C; +@M00176:17:000000000-A0CNA:1:1:14556:1818 1:N:0:0 +TTAGGCATCTCG ++ +B@?DFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:13347:1818 1:N:0:0 +GTTATCCGTACA ++ +=:BDDADDHHFF +@M00176:17:000000000-A0CNA:1:1:15755:1819 1:N:0:0 +TTAGGCATCTCG ++ +BCCFFFDFHHHH +@M00176:17:000000000-A0CNA:1:1:12743:1819 1:N:0:0 +TTTTGGTTTTTG ++ +007((((((2(( +@M00176:17:000000000-A0CNA:1:1:15278:1820 1:N:0:0 +TTAGGCATCTCG ++ +BCCFFFFFHHGG +@M00176:17:000000000-A0CNA:1:1:14225:1820 1:N:0:0 +TTAGGCATCTCG ++ +B@BFFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:13475:1820 1:N:0:0 +GGACCGAACTCA ++ +B@=DDF@@DFBF +@M00176:17:000000000-A0CNA:1:1:17441:1820 1:N:0:0 +TTAGGCATCTCG ++ +8=?B1+2+ +@M00176:17:000000000-A0CNA:1:1:18782:1827 1:N:0:0 +TTAGGCATCTCG ++ +BBBDFFFADHFF +@M00176:17:000000000-A0CNA:1:1:13786:1828 1:N:0:0 +TTAGGCATCTCG ++ +@B@FFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:13541:1828 1:N:0:0 +TTAGGCATCTCG ++ +B@@DFFFFFHFH +@M00176:17:000000000-A0CNA:1:1:16604:1828 1:N:0:0 +TTAGGCATCTCG ++ +@BBDFFFDHGHG +@M00176:17:000000000-A0CNA:1:1:18303:1829 1:N:0:0 +CGCTCTTTGTTC ++ +CCCFFFFFHFHH +@M00176:17:000000000-A0CNA:1:1:14128:1829 1:N:0:0 +CGCACCACGTGA ++ +C@CFFDD3D +@M00176:17:000000000-A0CNA:1:1:15154:1834 1:N:0:0 +TTAGGCATCTCG ++ +CCCFFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:13838:1834 1:N:0:0 +ATTGCCCCGATG ++ +?@@DBDD+@8CF +@M00176:17:000000000-A0CNA:1:1:16996:1835 1:N:0:0 +AGGTCTAGGGTC ++ +=@@+=ADD>=CD +@M00176:17:000000000-A0CNA:1:1:15945:1836 1:N:0:0 +GACTCAGCAACA ++ +B?@DDD3BAFFD +@M00176:17:000000000-A0CNA:1:1:15829:1836 1:N:0:0 +GTGAACTTGGGC ++ +B:?DDDEFHGHH +@M00176:17:000000000-A0CNA:1:1:18386:1836 1:N:0:0 +TTAGGCATCTCG ++ +BB?DFFFFHHHH +@M00176:17:000000000-A0CNA:1:1:12988:1836 1:N:0:0 +GTGGACGTCCAA ++ +=:=A==@DHHHD +@M00176:17:000000000-A0CNA:1:1:15728:1836 1:N:0:0 +TTAGGCATCTCG ++ +@CCFFFFDHGHH +@M00176:17:000000000-A0CNA:1:1:13129:1837 1:N:0:0 +GTTTGGCGACGA ++ +=1=BBDDDFHHH +@M00176:17:000000000-A0CNA:1:1:18507:1837 1:N:0:0 +CCACACCTTCTG ++ +BBBDDDDDHHHF +@M00176:17:000000000-A0CNA:1:1:14782:1837 1:N:0:0 +GATTTTTCTATG ++ +++1++,2+)))) +@M00176:17:000000000-A0CNA:1:1:15801:1837 1:N:0:0 +AGTACTGCAGGC ++ +<@BDDFBDBFHH +@M00176:17:000000000-A0CNA:1:1:16705:1837 1:N:0:0 +GCACTCGTTAGA ++ +B@?BB=288A?AB709@:3:A:C88CCD@CC444@>>34>>ACC:?CCDA@A>:? +@M00176:17:000000000-A0CNA:1:1:17088:1773 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGGACTGGAATTCAATGTG ++ +@@CDDFFFHFBHHIIIEHIBHIIIIGIIIIIIIIIIAGHGHHIHGGGIHHIIGGF65&7-2(3(+4:44:>C@ADC>:?>:>:@:>@>CA@CB@@++:C:ACACCC:<299>ABC:A3>A::>@C:>(20<.&0(+: +@M00176:17:000000000-A0CNA:1:1:16738:1773 1:N:0:0 +TACGTAGAAGACTAGTGTTAATCATCTTTATTAGGTTTAAAGGGTACCTAGACGGTAAATTAAACTCTAAATGAGTACTTGTTTACTAGAGTTTTATGAGAGAAGGAAGAATTTTGGGAGTAGTGATAATATACGTTGATCCCTGAAAGAC ++ +@@@DBD;;FHBHDGGIIIIIIGH@HEGGHHIII9+:*??FGIIII>D4)@@CCEHEEHDDE>ACECC3>C?A59ACB@CACC@:5><<23(>>ABC@CADCD8AC?<>AC9:4?>:((+ +@M00176:17:000000000-A0CNA:1:1:12561:1773 1:N:0:0 +GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTTCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGGGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTACCGGG ++ +@@CFADFFFHHHDHIIIIJIJJIIJJIIGHJJIJJGIJJJJJCGGAHHIJJJEGHH;C>DEB;ADDDA/;?@BDDDDCDDDDDDDBBDDDDDDCB12:?8?B509>3@BC4+4ABBDCC?B9<9<@B99HEIJJJJJJJIGGFIJGHGGHFHJIIIIIIIHCHIDIIJJGBGGIIEEEFFDDDCEEEDDDEA@;=;@DDDADDDCECDDB?CA@CCACCDDD>@CCCACD>CD@>>CBD@528<>>A??C>A?@:> +@M00176:17:000000000-A0CNA:1:1:12515:1774 1:N:0:0 +GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAATCTGGTGGAATGCTCTCGAAACCCATTCACTTGAGTGAGACAGAGGAGTGTGGAATTTCGTGTGT ++ +@@@DDDDDB66B5??+>AD8A@CBBB@?BBB?BC@:>CACECC:ABB28&9((+4@::AB:ACCCCCCC3::@3939?(8+2+4<3>4>A>92?(+2 +@M00176:17:000000000-A0CNA:1:1:17491:1774 1:N:0:0 +TACGTAGGGTCCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGCGCGCGCAGGTGGCTCGATAAGACTGGCGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCGTTGGGGACTGTCGAGCTTGAGGGCAGCAGGGGCAGGGTGGATTCCCGGG ++ +@@@DDFFFGHHFHGGIJJIJJJGIJIJHIIIJCHIJF'5@@CHEADDD>B@?A34>9@. +@M00176:17:000000000-A0CNA:1:1:16427:1774 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGTAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +@CCFFFFFHHGHHBHJIJJJJJJJJIJJJJJJJFIJ<6DHHHJJJGGIJIJIGHF=B6<<3>CACDCDDD38>:>+4>AC@C>A><((+448:>C(+4(4>4@<@>5>BD9(3:ACD>A@3(+:8@&)5>:@>9:AA::>CC(( +@M00176:17:000000000-A0CNA:1:1:13372:1775 1:N:0:0 +CATCAGGGTTAGGAACATTAGAGCCTTGAATGGCCGATTTAATACCAGCATCACCCATGCCTACAGTATTGTTATCGGTAGCAAGCACATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCGCCTCCAAACAATTTAGACATGGCGCC ++ +???DDDDDDDD3CEEEEEII3A3AEEF9EEICEE3DDDIDEIDEIIIIIIIICECDIIAE@>ADEACCDDDECD@AD@;?/>AA5=24:A??9;&5<9&50;>>>5<:45>>>;?>A(8<<:>A>A:>A>((+059 +@M00176:17:000000000-A0CNA:1:1:14806:1775 1:N:0:0 +AACTTGACTCATGATTTCTTACCTATTAGTGGTTGAACAGCATCGGACTCAGATAGTAATCCACGCTCTTTTAAAATGTCAACAAGAGAATCTCTACCATGAACAAAATGTGACTCATATCTAAACCAGTCCTTGACGAACGTGCCAAGCA ++ +CCCFFDDDHHHHHIIIIDHHBIIIGGHEDF+<<8?CF>BGG?9@6@GAHIGH9??GGGGGIGIIGGEBHGCEHHHFEBBBCDACCCEACCCCCDCCA@AA>C:C@CBBCCDC:@CCAACDDDC:3?<<1@CA>C>ACBD>>@>CC@A+8824259@D&)<>A@(:>:>(& +@M00176:17:000000000-A0CNA:1:1:18209:1775 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGGGGGCGGCCAATTAAGTCAAATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTGGCTAGAGTGGGGGAGAGGATGGTAGAATTCCAGGT ++ +BBCDFFFFGFDHGIIJIJJJIJIIIJFGIFIJIIGI607BFF<;FHFD''5785@@BBDDDDDDEECAACDEEDDCACDC?B9>DD>CCCDEDDDDCD@CCCDD@>CDD@CDCDDDBDD@84:@:++0&05.8<8(:<>CC@@AAAC>>3( +@M00176:17:000000000-A0CNA:1:1:16020:1776 1:N:0:0 +AACGAACAAGCGCAAGATTAAACATAGTGCCATGCTCAGGAACAAAGAAACGCGGCACAGAATGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGCTGCCCAGAGATT ++ +@@@DDDD?F?A+DG*?*0??B?FBFA;FH=BFED@4=DCBCCCC@ACCCC@CC::@:ACC>?5993<@B<:?CCCC?B@C>8?@@&)?:>>>4:>@:?:93:>((:(++(+:98<@???CA4 +@M00176:17:000000000-A0CNA:1:1:12460:1777 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCCCCCTAGAGTTAACCAGGGGGTGCTGGAATTCAATTGG ++ +CCCDFFFFHHGHHJJJJHJIJGIJIEHIJIJJIJIGHIJJJJIJJGIJHIIBHHFBB)078?3(:4@:>CCDCCDDD>B?:>@CAC>A:(+:(:@(:++:>3:>(+39>@88<&0990(++:>:@4(+48859@0&(+4(++:3+(:(((( +@M00176:17:000000000-A0CNA:1:1:17898:1778 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGCTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGATAATCAGGGGGTACTGGAATTCAATGTG ++ +@@CDDFFFHGHHH+@FHIJIJJJJJIJJJGHIHDHIB:FEEHIJI(=F;@G@@3:(:4@:<(4:::A:@(:(4424@:>(2>@C@(+:?3((2<<<08<349(+(+4>:::(:<@B00((:@9>:>3:3:>C> +@M00176:17:000000000-A0CNA:1:1:12607:1778 1:N:0:0 +TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGACATTTAAGTCAGGGGTGAAATCCCGGGGCTCAACCCCGGAACTGCCTTTGATACTGGGTGTCTAGGGTATGGAAGAGGTAAGTGGAATTGCGTGG ++ +?@@D1BDDHFFHFIIIIIIIICHIIIIIIIIIIIIIIIIIHH;BE?BCBCBBABB&))3>((+:AD4>(8;BBBCCC3>ABBBBBB8@CC<59@B5)&:AC>(:4>@C:@>CC8(0)4(+4(+3(4++((3+8?434:(4@(>@@3>9&95 +@M00176:17:000000000-A0CNA:1:1:16637:1778 1:N:0:0 +CACAAGTAAGACAAGTGTTATTCATCATTATTAGGTTTAAAGCGTACCTAGACGGCATTTTACACATAAATTGTCAATTTTTTGCTAGAGTTTAATAGGGGAGGATAATATTAGGACTATCGGTGTAGAGATGAAATTCTTTGATACTAAT ++ +@CCFFFFFHHGHHGICGIJJJJIIJIJJJJJJJJJHGGIJGIIGGIJJIJJJIEIJJJJJJIJIIEIIGIHHHHHHHFFFDFDDDDACCCADDDCCCDD@B@DDBDDDDDEDCC>@CA>CCDB?BBCCDDDDDDDCCCCEDA>ACDCC@:> +@M00176:17:000000000-A0CNA:1:1:12658:1778 1:N:0:0 +AACATAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTAGGTGGTTTGTCGCGTCGTCTGTGAAATTCCGGGGCTTAACTCCGGGCGTGCAGGCGATACGGGCATAACTTGAGTACTGTAGGGGTAACTGGAATTCCTGG ++ +CCCFFFFFHHHHHJJJJJJIIJJJJJJJJIJJIIJIIFHGIJJJJHHFEFDDEEDDDDDADDDDDDDBDDDDDEDDDCDDDDBBDDDDDCDDCDDDDDBBDBCA@BBD>BB@@BDDACCCACCCCCD=CAACC?<59ACDD>CCDACADDC +@M00176:17:000000000-A0CNA:1:1:14889:1778 1:N:0:0 +GTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGAAGAAGACTCAAAGCGAACCAAACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATCAGCACCA ++ +@@CDFFFFDHG?FGIIJJJJJJJJJIJJII9?FHHJJIIJJBDF;CBFHIIJIIIJJHIIGHACBBACBBDDDD:@BBDBDB>BB@BDDDDCDCCC><<8@B912BDDCD:A+>359@BDDAC4>ACD@>A>C@>A<<< +@M00176:17:000000000-A0CNA:1:1:14503:1778 1:N:0:0 +TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAGAAGGGGAGAGTGGAATTCCATGTG ++ +BB@FDDFFHGHHGJIJJJJIJGIJIGHGIHIIIIIJJIJIIIIIJJGFFDCBDBDBBBBCDECDEEED>@CDADDDDABDDACDDBB:CDDDDCDEEEDCBD03>>>@C3>CDCACACC@3>@A(+4>>(<939>9<:>?CCCCCD>>>C> +@M00176:17:000000000-A0CNA:1:1:15599:1778 1:N:0:0 +AGTTATTAAGGATGAGTTTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGAC ++ +@@:BDDDDFFF?FEFFA4CIHBFEEFFHFH+AACGFIGGIIIIEFEFFEIIGBFIFBFG>CAF;AEEFFFE@BDDCCCBA>BDBABBBB>AAB<@5>BB?8((:444:::>>4<9@FHGHI@CC=AAEDE?@B6@CDC?CC;A?;=9>?A>(4>CC>CC@C@11B3&(4:>>C08DC@DDC:<>ACC>CACCC>:A?:44@42:>C4(+:99ACA<9&9?B?>A@>3>CD:>CAC@B<&0>CA@ +@M00176:17:000000000-A0CNA:1:1:18258:1779 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGTGGTTGATCCATTGTTAAAAACATTGGCTTAACAAATGTGTGCATTGGAGAACTCCTCCCTTGGTTTAATCAGTGGGGACTGGAATTCTATTTG ++ +@@@DDDDDCCDFFDHH6EHHF?FHIHGHIIIIGGII@AFHGIG;BFAG5@BHCED/5&00&(+(++:43>AA>::C>()(+4((3:<>@A@:(4>(4@1((+:3:(33(+(++(+3((++(((+9:@>:+(+>@.851(8(4::4(+(((+ +@M00176:17:000000000-A0CNA:1:1:13050:1779 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGGTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGGG ++ +CCCFFFFFHHHHHJJJIHIJJJJJJGHIIIJJJJJIJIIJJIJJJJJJHHJIHHFCD7@D-??AC>C:ABCDD:>CC>?>ACCECDD>@>:ABDDDEDDD@@DACACC>@BBDBDBDD@?4:A@:A>3@@AB@@09CA@:(44>ACCAC(+ +@M00176:17:000000000-A0CNA:1:1:16097:1779 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGGTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHGHHGJJIIIJJJJJJIGHGJJJIJIIJJHIJIGGJIGHGHGIAEEBBB07@&>CCC4194>C:>C:>:3>CDC34>@BD>@@(4(+33@@ADDDDCC<35>9ACA((:4:@:A3444 +@M00176:17:000000000-A0CNA:1:1:12253:1779 1:N:0:0 +CACAACCAACCAGAACGTTAAAAAGCGTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGAAGGAA ++ +CCCFFFFFGHHHFIIJGH+AEHGGHI)?DGGHIIDGGEDF6AFBHIJE8;BEEFDECECECFEDCD;>:@AABDDD?B(88>ACC:AC?8AC@BB<@?C@(+:>?( +@M00176:17:000000000-A0CNA:1:1:13138:1780 1:N:0:0 +TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGCGTAGGCGGATAAATTAGTTAGAGGTGAAATCCCAGGGCTCAACCCTGGAACTGCCTTTAATACTGTTTATCTAGAGTATGAAAGAGGTGAGTGGAATTCCGAGT ++ +@CCFDADFHHGF>C?AACDC>AACC>?<@D8CC@(3(>@:3@(:::@+44:@A>34::@>>::(:932+8>?4:(4(+(::>9>2 +@M00176:17:000000000-A0CNA:1:1:14840:1780 1:N:0:0 +TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGTCAGGCGGGATTCGGTGG ++ +CCCFFFFFDHHHHJJJJJJJJJJIJJIIIIJJJJFFHIJJJJJJGIJHHFFDCEECECDDEDDDEEECCDDDDCDDDCCDCDDDD>BDDDDDBB3@CDC?@C:AB@@CCDDACCCCDCD:3>@335>&503(8882< +@M00176:17:000000000-A0CNA:1:1:17206:1780 1:N:0:0 +GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAAAGTGGCGGAATTCTCTCGAAACCAATTCACTTGAGTGATACAGAGGAGAGTGGAATTTCGTGTTG ++ +@@@FFDFFBB6B@@B>AADCECC:@@CCCCBCBBBCBBBCCCBCCB@@(4:AC><39<>A1?C@:>ACACA:((+(+:>@@?8(((++4((((+433(8((+ +@M00176:17:000000000-A0CNA:1:1:17334:1781 1:N:0:0 +CGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGGGGCTTAATATTCTTGGCACGTTCGTCAAGGACTGGTTTAGATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA ++ +?@?DBDDDHHDFB3CDH>HHE:FEHAEHIICBD==A@C=BCB?>CBCCCCDDCCCCCCCCCC:@AACCDE@@A::@3>@ACCCCCCCCBAC4@A4@>@((+89C@BB@8CA::>:@ACE@ +@M00176:17:000000000-A0CNA:1:1:17037:1781 1:N:0:0 +CATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTG ++ +BB@FFDDFHHFFHIJJJIJIJJJJJIJJJJJJIIIGIIG;FGIJHHHHGFFFCEEEEEDCCCDDCC>CDDCCDCDEDEEDDDCDDDDDDDDDCCACCCBCBCDDEDDDBBBCDDDDCDDCCBD@9@BEDCD>>CDCDCDD@CDDDDD +@M00176:17:000000000-A0CNA:1:1:16198:1781 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGGACTGGAATTCAATGTG ++ +BBCFFFFFHHHHHJJJJIJJJJJJJJJJJJJJJJIIJHHIJIJJJJJIFHJIHGFDB6;@+CCACDCEDD>CAD>C>CCDCA(>B8C:CDCCC<>?CBDD@AACCDCC>CA>A?@@&0.((89CCCC(44444 +@M00176:17:000000000-A0CNA:1:1:14924:1781 1:N:0:0 +TACGGAGGGGGTTAGCGTTGTTCGGAATGACTGGGCGTAAAGCGCGCGTAGGCGGATCGCAAGGTTGGGGGTGAAAGCCCAGGGCTCAACCCTGGAATGGCCTTTAAGTCTCGCGGTCCGGAGTTCGAGAGAGGTGGGTGGAATTCCGAGG ++ +@@@DDDDDDHFHFHHIFHIGIJ@DHI;;FC)=FHH9AEDEFD9<=>=@DBBB>BB-50;2+&&+>C?B3&505A?AB?A:@ADC4@>4+::888<@C:4@CC?<<2>A@@:A>A>CC@BB>5CC>@ACC><9BBBB9C<>?<>@B3:@( +@M00176:17:000000000-A0CNA:1:1:16914:1782 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGGACTGGAATTCAATGTG ++ +@@BFFFFFHHGFFGIGIIJJJJIIJJJJJGIJJIDID0?DFHGIEIIJ;@EEEADDD5<<8<AA:AC3>C>:@CC?CDCC4::@3>>CDB:(:::A>(:@>44?A3>+8<95AB3(3(4@D>:::@@:85>055(8(+4:@CC:4::( +@M00176:17:000000000-A0CNA:1:1:15172:1782 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHHGHHIJJHIJEHIJJJJJGGFIIJJJIJHGIIIIIJJIGIIJIHFEDD5CCDDA@CDAACDDD>A:>(4>ACDA@>448:3>C>3334>C(:AB<>:@(:>A>((+4>C +@M00176:17:000000000-A0CNA:1:1:13951:1782 1:N:0:0 +CGTCAACATACATATCACCATTATCGAACTCAACGCCCTGCATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCTCAATAGC ++ +BCBFFFFFHHHFGIIJGIJJJJIJJIIJJIJJJIIIJJJJIDGGIJIJJJIHIIJJJJJIJHHGFFFDFFFFECEECEDDDDBDDDDDDDDDDDCDDCC@CBB?AABBCCCDDDDDDC@A?:9ACDCCCAC>>:>>@:1<8C: +@M00176:17:000000000-A0CNA:1:1:17573:1783 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGAATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCAAGCTAGAGAAGGGCAGAGGGTGGTGGAATTTCCTGT ++ +@@@FFFFFGHHGHJJJJJJIJJJJIJIJJIJJJHII66@FHIIJJJIF9ACD;CBDDDDACDDDEFDDCDDDEDDCDDDDDDDDDDDDDDDDBDBDDDCCDD@CACCCBBCC?BCA@BC344+(+BDD(8?@88>@DDD( +@M00176:17:000000000-A0CNA:1:1:18622:1783 1:N:0:0 +GTTAGCCTCGGTACGGTCAGGCATCCACGGCGCTTTAAAATAGTTGTTATAGATATTCAAATAACCCTGAAACAAATGCTTAGGGATTTTATTGGTATCAGGGTGAATCGTGCCAAGAAAAGCGGCAGGGTCAATATAACCAGTAGTGTTA ++ +??@DDDFFDFFFHDGIHIHHGHGIJGHGIIB@DHGH.)//8=C=CHHEHE>EACCFFFFC>DEEDED?=?>@CA?@ACDD@CAC593>C@@@@>AACCDD>>(<++::@AAB??C>B?CCDC1<0&0&05ACBADECE((+(4>>A@: +@M00176:17:000000000-A0CNA:1:1:13264:1784 1:N:0:0 +AGTCCTTGACGAACGTGCCAAGCATATTAAGCCACTTCTCCTCATCCTACGCGTCAGTTTTTGACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGTGCTGCGCAAGGATAGGTGGAATTTTTTCATTTGCTGCCA ++ +::?44BDD<>DAD1@CGDDBC?B9B89**060@E/'57A@@DE<(6>CA>-,,;58@@A:@;@9955&08>49>55&>(:4444(+(+44<(((+4(0&&&02((49C4(8((((+(()((+,4(:44((( +@M00176:17:000000000-A0CNA:1:1:19051:1784 1:N:0:0 +TTTAGTGAGTTGTTCCATTCTTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAGCCACATATAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTTGAGCATCAACAGTCCACAACC ++ +@@@DDDDDHH>+CC::((45&0&&4>8CA+4>CC4@CBB< +@M00176:17:000000000-A0CNA:1:1:18234:1785 1:N:0:0 +AAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGTCTTTTTTATGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTGATTA ++ +@B@DDFFDGHFFFGI@HIICDCBBDDCCA@>BD?BCCCDACCCDAC:>C:>>:A(+515>>>@+ +@M00176:17:000000000-A0CNA:1:1:18726:1785 1:N:0:0 +CGTCACAGGTTGCGCCGCCAAAACGTCGGCTACAGTAACTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCGTCGCGTCGTAACCCATCTTGGTAAGTTGGATTAAGCACTCCTTGGACAGA ++ +=@:ADD8DAHHD8<42A@C@@(;A>>A@3=;814>3(+++>>:A>4>?CC325<&)))&)0<>>@:>:@4+43(44(+4::43>>3(:>3::3(( +@M00176:17:000000000-A0CNA:1:1:12958:1785 1:N:0:0 +TACGTAGGTGGCAAGCGCTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGATCAGTTAGTCTGTCTTAAAAGTTCGGGGCTTAACCCTGTGATGGGATGGAAACTGCTGATCTAGAGTATCGGAGAGGAAAGTGGAATTCCTAGTG ++ +B@@FDFFFHHHHHIJBIHIJHIIIJIIJIJJJJIIGHGHIIGIIHIDDDBDDDDD98B?CADCCDDEDDDDC@DCCCCDEDBBDDDDCCDA?BC@CCDDDDCC?8>CCCDCDDDC@>C4>@C+44>?9BD.???8A?:@CACCCACCDCDA +@M00176:17:000000000-A0CNA:1:1:18601:1785 1:N:0:0 +ACCAATCCGCGGCATTTAGTAGCGGTAAAGTTAGACCAAACCATGAAACCAACATAAACATTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGGATAAA ++ +@@@DFFFFHHHHHJJJJIJGHFFHJHHIIIGHIGCHA@FFAHIJJBDHCHFHFFFFFDEECECEECDCDDDDDDDDBDDBB@BBDBDDDDDEED>@CCDCBA>>:ACDCDDB5?@CDDCCDDDDDB3>ACC>8935<@CDDDCA3((:@@: +@M00176:17:000000000-A0CNA:1:1:18699:1785 1:N:0:0 +TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGCTCGTAGGCGGTTTGTCGCGTCTGCCGTGAAAGTCCGGGGCTTAACCCCGGATCTGCGGTGGGTACGGGCAGACTAGATGGATGTAGGGGAGACTGGAATTCCTGGG ++ +@@@DBDDDA?F@EHE@FHGGCA4@GGGCHHEEBEEED3>88?BBB>&8<>BBBACB>>BB:A>AC@0-5@5B99544>0))9&593499&505(2:49@(4>CCCCADC?9&05(((23:>(+:::9+ +@M00176:17:000000000-A0CNA:1:1:18674:1786 1:N:0:0 +CTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCGCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGAGGAGGATAAG ++ +@@@FFFFDFHCFHIIIGIGEHIG??8CHC6?67@GCEHHHAGH>@CEF:CBB@CEECE>;9@;;'::@-CC-9BBB@@CECC@>99@CCC?&0+(9A:C>3::84:@CD(419@CC4550)0&(((++2&((+4> +@M00176:17:000000000-A0CNA:1:1:12910:1786 1:N:0:0 +TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCAGGCGGTGACTTAAGTGAGTTGTGAAAGCCCCGGGCTTAACCTGGGAATTGCATTGCATACGGGGACGCTAGAGGACTTTAGGGGGGGGGAGAATTCCACGT ++ +@CCFDFFFFFHHHIIIIIID:BGHIIIICDADCCDDD-55CDC4(((4>@A((9&&)555(&(+(+(+444::<&500&&)098@A>@@A?< +@M00176:17:000000000-A0CNA:1:1:19142:1786 1:N:0:0 +TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCNNNTAGGCGGCTTTGTAAGTTANAGGTNANAGCCCGGGGCNCNANTCNNNAACTGCCTTTANGACNNNATNNCTNNAANCNNNNNNNGGTGAGTGGAATTCCGAGT ++ +@@@DADDDHC>BD>#+2::#(+2###(+##++##+(#+#######+++28<(+8:4:44:>9<8 +@M00176:17:000000000-A0CNA:1:1:13222:1786 1:N:0:0 +GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTTCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGT ++ +@@CFFFFFHHHHHJJDIJJHIJJJJJIIJIJJIJJGIJJJJJJJJJJIHGIJJJHGHHFFFFFEEFEDDDDDBDBCDDDDDDDDDDDDDDDDDDDBBDDBBBB@<?B5 +@M00176:17:000000000-A0CNA:1:1:13401:1787 1:N:0:0 +ATAGAAACCAACAGCCATATAACTGTTAGCTTTACGCGGCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAACGTCCTTCGTGTAGCGAAATGCGAGGGGCATACTGTAACCATAAGGCCCCGTATTTTGCAAGCTA ++ +@@?DBDDBDCC;:2?(5?@7=8,53(3922(:>?))-+8??(4<3<<3((&)0&+((&2&&5>.9?>A:(+>::>3>3(+:91+55&&++4(+4>A:?A4 +@M00176:17:000000000-A0CNA:1:1:16358:1787 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHHHHHEIIJJJJJJIIIIJJJIJJJJJIE?@FGHJIDHGIIIJHCHDDD-7B@CDDAC>>CCDDD>((:4@CDDCCCDB7>55@@CCDDDDDDDDD)(:99<9>A:A3:<><@BD8AB?:A@@A>A9( +@M00176:17:000000000-A0CNA:1:1:15041:1787 1:N:0:0 +TCAGCAGCCAGCTTGCGTCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAACCATTTTTCGTCCCCTTCGGGGCGGTGGTCTATAGTGTTATTAATATCAAGTTGGGGGAGCACATTGTAGCATTGTGCCAATTCATCCATTAACTTCT ++ +B@CDDFDFHHHHHIJJJ1@FHJIJJJJ@:CGIIGFHGIIGHEHGIIIJJIJHEHJHHHHHHFFFDDDDDDDBDD=@B<5>@BCCDEE@CDCDDEDEEEEEEEDD@ACDDBBDBDBDCDCDAC>ADDC@@@:@CDDDCCDDCDCDCCCCDD@ +@M00176:17:000000000-A0CNA:1:1:18163:1788 1:N:0:0 +AATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT ++ +CBCFFFFFHHHHHA>ACGIJJJJJJJIGJJF>GHIJJIIIGGJJIIIGIJHICHIIIJJJIIIJIEGGGGGHGJHHHFFFDFDDDDDDCDDDEEEDDDCCDCCCCCCACDDCDDDDA@C9?CDDDDCDDEDDB@C:>>@>:>BB?9<82@C@:44@>3>>CC@>A<.+8?:>CC<>(:ACC3:::A +@M00176:17:000000000-A0CNA:1:1:16538:1788 1:N:0:0 +CTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTG ++ +CCCFFFFFHHHHHJJIJJJJGIGGHIJJJJJJJJJFBBDDGHIJIJIJC8CGGGGIJJJHGDFBFEEEDEEDDDDDDDDDDDCDDDCDDDDDBDDDDDDDCDABBB?>CBDDB7>BB@BD@CDD?B@@>@BCC@C>C@>>@C@9?@CCDD: +@M00176:17:000000000-A0CNA:1:1:17725:1788 1:N:0:0 +TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGT ++ +B@CFFFFFHHHHHJJJJJJIJJJIIJIJJJJJJIJIB:DGHGHIGFGGIFGHHIGHGHHFFFFDEEEDCDD;@BDDDDDDDCDDDDBCCCDDDDDBDDDDBB@505A:ACCDDDDCABCD<8@DDDDDB@@@CDCCCACCCCD443(4>(44 +@M00176:17:000000000-A0CNA:1:1:14132:1790 1:N:0:0 +TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGGTTTTTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGAAAACTTGATTGCAGAAGAGGAAAGTGGAATTCCATGT ++ +@@CDDFDDHHGHHJJI@GHEGIGHIIJCHDIGGGIFIIIIGIJJJJHFFDDDABBDDDDDDBCDEEEDADDEDCECCDBDBBDDBBBCDDDDBBDBDCCC:?CCACCCDA@(+:>>C@CCCD>ACDDD:3:+ +@M00176:17:000000000-A0CNA:1:1:15137:1790 1:N:0:0 +TTCTTTGATTTGGTCATTGGTAAAATACTGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGACGCAACGCGAGCAGTAGACTCCTTCTGTTGATAA ++ +@@@DDDDDHHH8C2ACGHHHCIEHIIIIIFHIIIIIGIIGIFEEEHHEEBC?CCCB?CCC@CCC>CB3::?CCCC?+8?ACCCACDAACBBB@B>BBB.9@C@>:AAC@@CACCCE:>@CD3 +@M00176:17:000000000-A0CNA:1:1:15656:1791 1:N:0:0 +GACGACTTCTACCACATCTATTGACATGATGGGTCTGCAAGCTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTACTGCTTGAAAAAAAAGAAAAAGCAGCAGTGAACGGAAGTGTTGCATAC ++ +???4=@DDDDDBD+C?AADD:A(6.;(55;3-5-55,5((())&+(43+:>A(8((425>9&((+((&(+9((+4((+(+&++&+++((((+(( +@M00176:17:000000000-A0CNA:1:1:18805:1791 1:N:0:0 +TACGTAGGTCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGATGTTAAAGGCTGTGGCTTAACCATGGTAGGCTTTGGGAACTGGTTAACTTGAGGTCATGAGGGGGGAGGTGGATTCCATGTG ++ +@@@DDDDDH?)@F'8=@'5<55&8(((+3@B:>:(4>(:@C3:?4<+9<<@?>:>>4>(3(+(((+3+:9&23(+:(+4>(:(4+(4(+(((4(++89&505&(2((+(:((43+4 +@M00176:17:000000000-A0CNA:1:1:12765:1791 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGGAGGTGGTTTGTTAAGTTGAATGTGAAATCCCCGGGCTCAATCTGGGAACTGCATCCAAAACTGGCAAGCTAGAGTATGGGAGAGGGTAGTGGAATTTCCTGT ++ +?@@BDBDDADFFHIG=EHIGEHIGIDCGHIGHIGG@DCCACACD>@C@4+:(559BBB::::@CCCB?(4(4>>C9:44(2>A?89481@:@>>AA@AC(+20<798(+:>1+(::4@CCC: +@M00176:17:000000000-A0CNA:1:1:14262:1792 1:N:0:0 +TATGCATGGGTGATGCTTGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGTCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGT ++ +=?+=BDDDDDDDDIIII>ADEAAAAABAA>>?>AADAA??A3::::>>9>AA>3>>>>>?>?>>A<2;(25<<3<>A::>:59 +@M00176:17:000000000-A0CNA:1:1:16040:1792 1:N:0:0 +GTTGACAGATGTATCCATCTGAATGCAATGAAGAAAACCACCATTACCAGCATTAACCGTCAAACTATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAGAACCAGC ++ +@?@FFDEFDFHHHIIIIIIIGIIEHDIGGGIDHE21::C=DEIIGGGIIIIAD?B>FDHHIIIIIHGIIIAGHEHGHHFBBD??CBCDB=CD=CCDDDDDDC@CC@CDECC?CBC@@?BBBD@3((4:<8++9@>CCC>C4@@(:4:8<BDD8ACCC3>>C@?.9<A::A>4>+ +@M00176:17:000000000-A0CNA:1:1:17296:1792 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGGGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCTGGGGGGACTGGAATTCAATGTG ++ +CCCFFFFFHHHHHGIJJJJJJJJJIGIJJJJJIJJJJEGIDGGICCGHHGIIGHFBD-7@050+(+>3:C4@C(>AD3AACCACACB:@:A+8@>@C@@(393:18>0::>@AC(+>AB@&&)::@::34:(( +@M00176:17:000000000-A0CNA:1:1:16119:1792 1:N:0:0 +TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGCTTTGTAAGTTAGAGGTGAAAGCCTGGTGCTCAACACCAGAACTGCCTTTAAGACTGCATCGCTTGAATCATGGAGAGGCGGGTGGGATTTTCTGT ++ +@@@DDDDDFAHHDGFE@GGH;DFFGGIIIIIIIEGGIHFBBEECC>@BBCB<5;-5@BC3:@C@DCCCCCB+8@CC>CBCAA:(43::+:ACAA3@CCAA>BB22<(:>:@C(:(3(30& +@M00176:17:000000000-A0CNA:1:1:15866:1793 1:N:0:0 +TGGTCAGATTGGTCGTCTTATTACCATGTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGGCGTGGCCTTGCTATTGACTCTACTGTCGACATTTTTACTTTTTATTTC ++ +:=+4=DDAFFDDAEGC?FGIGIGCCHG4AEH>C?11119CDDB8><>@-8@()2?1<:@CC@>:(449&&009&084>>@C:@(4:A@34:(:44((+)&&44+:144(+4((((++ +@M00176:17:000000000-A0CNA:1:1:17157:1793 1:N:0:0 +TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGGCATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATACTTGAGTGCCGGAGGGGTAAGCGGAATTCCTAGT ++ +@@@FDEFFGFHFFGGEGEGGFDFHGHEHIJFIIDG.--=FGHEBGGBEFF?BCCDB<>@BB<>?:AAAACDE>>AAC?AACDDBDDDDDCDA<4:(44:ACDDC3(4A39AC4>A:@CCD<3(+>(:@&055<95>BB.5<@A>3(4+ +@M00176:17:000000000-A0CNA:1:1:18753:1793 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGGGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGTAGATCGCCTCCCTAGAGTTAATCAGGGGGGACTGGAATTCAATGTG ++ +@@@?DDDDHFFFHGIGGGFAFEGEHIJJJEHIGIGGHDFGDGGGIIIBGEHEBHFB8&57<@-+((4(:>:CC:@:@:8?CCC:@>:>4>AA?A@@C4?>A@>(++434>28@50<@<((+(+4+++(++(+5.&)9?<834(+43++((( +@M00176:17:000000000-A0CNA:1:1:15428:1793 1:N:0:0 +GATGATTTCGATTTTCTTACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGGTTATGGTACGCTGGCCTTTTGAGGATACCCTCGCTTTCCTGCTCCTGTTGGGTTTATGGCTGCCGTCT ++ +@@@D@DBDFF1DDE>F???B=;F:ACHAEF3C?:CF9DCFGBF8,;@BA9>@>50+5&(+44>3((29A9AA>4>?<328@:A3(+:>:>::(>()(+23>(4(38<<9<>2 +@M00176:17:000000000-A0CNA:1:1:12586:1793 1:N:0:0 +GTTATGGTTTCCGTTGCTTCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGATACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTGGCATACTGACCAAGA ++ +@?@FDDDDFFCFACEEGF:AEFEHIIHE9F9F?FHGI?BFHGHG9F0DGGGBFGHF=)))/=@CH3@AEEAE;?BD/@A>AAB?B@8@C5>CACACCC>CAC34(>::AA>C>CC:>:C:2(444(4@C4@((48@CDD>AA:4:4:(3(((05>.<<83+(:>@>>(((:(>A>>)&:@CD3>@>::>AADE +@M00176:17:000000000-A0CNA:1:1:12353:1794 1:N:0:0 +AACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTTCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGG ++ +CCCFFFFFFFFHFIIJJJJGIJJIJJGIIJJJJJJGIHIJIIJJCFHH@GHJEHHEHHFFFFFFEDAC@BDDDDDDDCDCCCBDDBADDDDDDCD8BDC?@CD?@C80>A?8?43>BB98@C480& +@M00176:17:000000000-A0CNA:1:1:18498:1794 1:N:0:0 +TTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGAT ++ +@@@FDFFDDDHDDACCCHGGHGHGIIJJIIJJH@FE/00?BDHAF8BH>7.7=A;CDBCFFCD7;=;>?A9>A:@CDC>CCCC@@C4>>CDCA@AACC@CCC18?BB@BBB3>AC@@@@.809BC> +@M00176:17:000000000-A0CNA:1:1:15687:1794 1:N:0:0 +TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTAGGTGGTTTGTCGCGTCGTCTGTGAAATTCCGGGGCTTAACTCCGGGCGTGCAGGCGATACGGGCATAACTTGAGTACTGTAGGGGTAACTGGAATTCCTGG ++ +B@CFDFFFHHHHHJJJJJJJJJJJIJJJJJJJIJJJJIJJGIJIHHHFFDDDACDDDDDDDDDDDDDDDDDCDEECDCD:CBDDDD@DDDDDDBD@D>BBD?A?>BDD@BDD>B>CDCCCCCCAA@::@4:@:?>5<9:CCC>@CAAC>A9 +@M00176:17:000000000-A0CNA:1:1:14375:1794 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCAAGCTAGAGTATGGTAGAGGGTGGTGGAATTTCCTGT ++ +BCCFFDFFHHGHHJJIJJJIHHIJIJJJJJJJJJIIIJJJJDHIJJIFFDDBCCDDDDDDDDDDDDDD@CDCDDECCCDDDDDDDDDDCDBDDDD=CBCCDDDD9AC>ADDACBC@?BC>A>@:3:@?@>3::ABB?9>BDB:CDCA>C@: +@M00176:17:000000000-A0CNA:1:1:18935:1794 1:N:0:0 +ATCTCGAAGGAGTCGCCAGCGATAACCGGATTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGG ++ +???DDDDDDDD3CEEIII6EEEIIIIIIID)@DEIII9CCECEIIDEIE9AE6AA@D@AAAEAAAA??A????A?A?????AAA?>A<>?A>AAAA?>>?>AA?>???;>???;34>AAA>ADDBADAA34>AADDAB>3:>A:>>< +@M00176:17:000000000-A0CNA:1:1:16822:1794 1:N:0:0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCGAAACTGTCAGGCTGGAGTCTTGTAGAGGGGGGTAGAAGTCCAGGT ++ +@@CFDFFFHDHHG@GGJIIDHJJJJJJJJJJIJIIJE;AHHIIIFIFFEFDCBBD=@B?>ACDDEDDB5<>CDCACDDDDDBDBDD?@>CDDDDDBCDDDCA>A:A>????&2>:+4@CCDBB<<&0<:>(44@>CC: +@M00176:17:000000000-A0CNA:1:1:19069:1794 1:N:0:0 +TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTTGAGTACTGTAGGGGCAGAGGGAATTTCCGGG ++ +BBCFFFFFFHHHHJJIIJJHIJIIJIIJIJJJJJJEIJJJHJIJDGHGGGIGJJGFHHEDFEFFEFADBBBDBBBDDDCDCDDDDD?CCDDBDDDB<@?ACC@:9>:+8@ACDDDDC3>::3>09505288@B?BCDDCB<9 +@M00176:17:000000000-A0CNA:1:1:16972:1795 1:N:0:0 +TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGTGGTTTATTAAGTCTGGTGTAAAAGGCAGTGGCTCAACCATTGTATGCATTGGAAACTGGTAGACTTGAGTGCAGGAGAGGAGAGTGGAATTCCATGTG ++ +CCCFFFFFFDDHAHHIJJIGGIGJJJJJJIJJJJJJJ?BBDCDDCCCDFEDDDDDDEEDDBBB@CCCBBDDDDB?CCDD>>CCDDEC@CDA?AAC4434>@C>:C((+::?B?2(88<8?:(8:@CDDCDDCCC +@M00176:17:000000000-A0CNA:1:1:17236:1795 1:N:0:0 +CGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACG ++ +@@BDDFDFHHHHFJJJIJJJJJIJJJIIIHIIJIGIGIIB@FHIIJJJICDEGIJJHHFFDDDDDDBDDDDDDDBDDDDDDDDDDEDD9AC@>A@CCCCDAC<((:+44((((((4:((4(+(+><+(4>:((+(+((+)&&&)&))&+8&(++8+4(+(+4(2539.)58(:(:(44(+((+:( +@M00176:17:000000000-A0CNA:1:1:18132:1795 1:N:0:0 +TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGCAGGCGGACATTTAAGTCAGGGGTGAAATCCCGGGGCTCAACCTCGGAATTGCCTTTGATACTGGTAGTCTTGAGTATGAGAGAGGTGTGTGGAACTCCGAGT ++ +@@@DDDDDDHGICIIIAHIIIIB;BGHF9CDEEC'9?B@BB57@:>BDDCAD(>32;BBBCC:4@:@9@BBBBCCCBA?8>:34@>:@ACDACC:+(444:@>>ACA>@CCC@BDDDCCDDDDDD9>>05>B3>>>BBBBDDDB@<@:>>>CDC>CC:::(4:@9599>@C?CAC:AAA>C +@M00176:17:000000000-A0CNA:1:1:16136:1796 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGAATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCAAGCTAGAGTATGGTAGAGGGAAGTGGAATTTCCTGT ++ +B@@BFFFFFHHFHJJJJGIJJIJIJIJDHGIDFH?GIHFEFGH9<@1:@?@CA@@BDDCCC@CC(>AA88@3389ABBCCDCDDDDEDCA3(425&+2>::@3:@CA4:>4 +@M00176:17:000000000-A0CNA:1:1:17012:1796 1:N:0:0 +TCATATCTAAACCAGTCCTTGACGAACGTGCCAAGCATATTAAGCCACTTCTCCTCATCCAACGCTTCAGTTTTTGACAGAATCGTTAGTTGATGGCGAAAGGCCGCAAAGTAAGATCTTCTCGAGCCTCGCTAGGATAGGTCGAATTTTC ++ +??1D=4BADDDDD+A<:?BE4FEFDEA?E?):??BD)0090?D?DDD>B?B88BDD)=)).)7(5((97).;?A=?;5;A>>>A5<9:<;;>>.2((0990&94:>>>(+:>AA((+&&&(+0&(+0+4++9(:(0&+:>AA: +@M00176:17:000000000-A0CNA:1:1:14327:1796 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGGTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGCGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHHHHHIJJIHIIJJJJJJJJJJJJJJJIJIJJGHJJFIJJIIJHHHFDD-5;&8?ACAC(4>:@C>CCC38(+:C3>@C(:4:@84::A@@B&)9::(+(3>2?@5A>C>C:4:<>705@CACA3(4@3>AC@: +@M00176:17:000000000-A0CNA:1:1:14533:1797 1:N:0:0 +TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGCCATTCAAGTCGGGGGTGAAAGCCTGTGGCTCAACCACAGAATTGCCTTCGATACTGTTTGGCTTGAGTATGGGAGAGGTTGGTGGAACTGCGAGT ++ +@@CFBDFDHHHHHJJJIIJJIJJJJJJGJJJJJJBFHEGCHEHCDDDDDDDA@CBDDD@ACDCC><(32088@BDB?@B243>@A9590 +@M00176:17:000000000-A0CNA:1:1:18569:1797 1:N:0:0 +TTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTA ++ +@@@DDBDBFHCCAFHIHIIIJJIAACCDDC>C>ACBB:;=?>>CDDC:CCCC:@B720@@>CDC(4:@C@CB?((&+2+:@AC@@>::((4>>982?A::@A>@> +@M00176:17:000000000-A0CNA:1:1:14919:1798 1:N:0:0 +TAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTTGGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGCGTTATTTGAGTATCTATAACAACTATTTTAAAGCGCCGTGG ++ +???DDB??5:>,>A>>:<>AAA4:(:3+4(+(&++&2:>(:(+:>:B:>>>3(4(((+:3:3+++:&09>595 +@M00176:17:000000000-A0CNA:1:1:18955:1798 1:N:0:0 +TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTAGGCGGCTAATTGAGTCGGATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTCGATACTGGTTAGCTAGAGTGTGGGAGAGGATGGTAGAATTCCAGGT ++ +B@BDDEFFGHGHFIJJJJJJGIJJIIIJJJGIJJJIJJJJIIJJIGGFFFDCEDDDDD>CC:>CDDDD;BDDDEECDCACDD@BDDDDCCDDDCDDABDDDCA@:@AA8>CD:@C:BCC>C:ACD4@?BA79<@<9><@:>CCC@C@:@<+ +@M00176:17:000000000-A0CNA:1:1:14684:1798 1:N:0:0 +ACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTT ++ +B@+ADDFFHHGHHIIHJJJJIGIJJFFHGI4?FHG?DGFGG?BGDHIJJJIFGIIHHIJJJIJJJJGFEEHG@ECHHEHFHFFD?BBBDCD>CC>CDBADCCCDDD?@BACDCCCDDCCC@CDCB:(4:&)5959?C:>C:ACBDDAACCD +@M00176:17:000000000-A0CNA:1:1:16933:1798 1:N:0:0 +GGTAAAATACTGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGACGCAACGCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCTCATTTTGT ++ +@@@DDDADHHFH8CGHAGGIF@BDCGHIIIE9?@@F9*:D?DG>CCC;@C@B3;@C@:8A@CCCCEECCCDCCC9<.59<.9.&55CCCC3(:>A@@CC>C34:@>>@CCC?B?ACCACED4(4+ +@M00176:17:000000000-A0CNA:1:1:15580:1799 1:N:0:0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGAGACTGGCAAGCTTGAGCCTCGTAGAGGGGGGTAGAATTCCAGGT ++ +@@@BDDFFHHDHHGHGIJJGIGIIJGIDHIJJBBFBFGHIIEGEDHFFFFB8?BBDDDD8A??ABCCDD4:AA@(+>CC?BDDD@@@(8@ACD?@8??DDD.CCDCACCC +@M00176:17:000000000-A0CNA:1:1:14654:1799 1:N:0:0 +CGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTT ++ +@CBFFFFFBHHFDFHGIIJJHIJJJJJGIGGHIJIJGGHIDDBFHIGIGIJJJIJJJJIIIGIIIHHFHHFFFDCEEDCCEFDDDCCCCDCDEDACDCCCDDCCCDCDDEDDB??BD@@@BCCDCCCCCCDDDE@(:@CDDCCD34:>3>@ +@M00176:17:000000000-A0CNA:1:1:17702:1799 1:N:0:0 +GACGATGAGGGACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCAATGGAGAAAGACGGAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTAGTTGAAATGGTAATAAGACGACC ++ +@BBFFFFFHHHGHJIFIJJIJDHIIJJJJIIGIIJJ4:DC?BHHIJHGEIIHGHJJJJIJJHHFEFFEEEEEDDDDDDDDDDBDDDD@?BDDDDDDEDDD8C380>?BDDDDDDDCABB@@<9>>ACCA>CA@AC:ACADC:93.& +@M00176:17:000000000-A0CNA:1:1:14715:1799 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATCGGGCGTAAAGGAAGCGTAGGCGGGGGGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHHHHHBIIIIJJJJJJJJJJJJJJJJJIEGHHIGIHJJIHHHFFFABBD&0;&5@>C@C3:@@@A:ACCAA>3>@@C3>:>::A?CC(::2:::A:C>AA3>??59>ACC>@?>B<&:ACCC:A:>CCCA>@ +@M00176:17:000000000-A0CNA:1:1:17454:1800 1:N:0:0 +AAGAGCTTGATGCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCTCAATAGCAGGTTTAAGAGCCTCGATACGCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAG ++ +@@@DDEDFHFHF?<@2@@FHGGIDGAHHIHG9F?9C@3BG??FHHGJJIGIEA9FC@FDGHIJJ>EEFHHFFCDECDCDDCDDC@ACCA9@CCDDCDDDCCD.>2>@ACCDACCC@C<(08(:@CC@CC@9A<(33:>4: +@M00176:17:000000000-A0CNA:1:1:12800:1800 1:N:0:0 +TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGG ++ +CCCFFFFFHHHHGJJJJJJJJJJJIIJJJJJJJJJIJHIJJJJJJJHJIJJJIIGHHHHFFFFFEFDDDBDDDDDDCDDDDDDDBDDDDDDDDDB@BCB<@DBBDDBCDCD@CCCAB@CCCB>@@C@B9>?ABB3ABCDC@<55 +@M00176:17:000000000-A0CNA:1:1:16784:1800 1:N:0:0 +TACGTAGGGTGCGAGCGTCGTCCGGAATTACTGGGCGTAAAGAGCTCGTAGGTGGTTTGTCGCGTCGTCTGTGAAATTCCGGGGCTTAACTTCGGGCGTGCAGGCGATACGGGCATAACTTGAGTGCTGTAGGGGTAACTGGAATTCCTGG ++ +??@A=DDDDHHHD1CGIHGDHGHIIIIIECDHIIIIBBGHEHEEBBDECCCBCCBBBB5<:89B@BCACDCC>CCCBBBBB8@CCC@@BB>5<<8<59>?:(+:@>@((+(:@::@A@7&09@:@(34>CCCC@9 +@M00176:17:000000000-A0CNA:1:1:17909:1801 1:N:0:0 +TTCTCATATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGTTGGAATTGGCTCT ++ +@@@DDDDDFHBF8222<@FC9B*BFDFHIBGGIIB(;C)@EFECCHEE@@BCD@C;AACACDACCBCCCCCCC6'5<.:>>(:A>A(>@3:@A((::13<>BBB0:>3+>(+:8++:CC3>@>3 +@M00176:17:000000000-A0CNA:1:1:15637:1801 1:N:0:0 +TCCTCATCCAACGCGTCAGTTTTTGACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGAATTTTCTCATTTTCCGCCAGCAGTCCACTTCGATTTAATTCGTAAACAAGCAGTAGT ++ +CCCFFFFFHGHHDHIIJJJGJJJJJJJ>GHCHIJIJGBFHF?FCBBF@EGHDHHFFDDDD@;CC<>CCCCDDBCDDBDDDDD35CCCCCDA?ADC?((:@D???BBCCDC??C4:@( +@M00176:17:000000000-A0CNA:1:1:14448:1801 1:N:0:0 +CGTCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTAAGCACTCCGTGGACAGATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTG ++ +=@+=BDDDHHHHHGHBGGGDHIGFHGGGG@@DDCC@ACCCCCADACCDCBBB@9BC@>5@BB>>C@CDED@(4>C@CC@CBCCCCC:A<<>?+>:A>59&58ACCB32((+2((+:CA3( +@M00176:17:000000000-A0CNA:1:1:12483:1802 1:N:0:0 +TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGTGTAAAGCGCGCGTAGGCGGTTCGTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGCGGACTTGAGTGCAGAAGAGGAGAGTGGAATTCCATGT ++ +BB@FFFFFHHGHDGIIIJJGIJIHGGDGIIJJJJJ0@GHIIGEHFFHHFDDDABDBBDDDD??CEEED@CDEDEACDCDDD@BDDDBDDDBB>3<.9BDBA@AC>@4:ACC@CDD7)00:@3:AC@AAA>?3AA>>@:BA4 +@M00176:17:000000000-A0CNA:1:1:12930:1803 1:N:0:0 +TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGT ++ +B@CFFFFFFHHHFJJIJJJJH@HGIIIJJIIIIJJIJJJJJIIIDEIIIJJJIJH@EHGFFDFFECACDBBB?@BCCDDDDDDDBDBDDDBBBDDDDABBDD5<3.(+:?CACCCCBDA39908?<9AB>CD@BB& +@M00176:17:000000000-A0CNA:1:1:14068:1803 1:N:0:0 +CTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGC ++ +CCCDFFDDFHHHHGI?FFHIHIJJ>GG>GCFFIIGGBGEHIIJJGGHHIIICHHDDDEHIIEHHHHFFFF>@CECCBBDDDB?BBDCBBBDBB>2><@B3<<>>BBACDA>@C@<@BBA2:@CDCDCA444>:((+59?B?C<3@: +@M00176:17:000000000-A0CNA:1:1:17421:1803 1:N:0:0 +CCTACATACCAAAGACGAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGCACGTAATTTTTGACGCACGTTTTCTTCTGCGTC ++ +CCCFFFFFHHHHHBCGFGDH@GH@FHIIIIIIIIGGIIIBBBB>BCCCBCCBBCCCCCCCCCCCCCC@C>CCCCCCCCCCCCCCCB8<@@>9??CC?3:@2?CCDBCCACCCDC@@CB40+ +@M00176:17:000000000-A0CNA:1:1:13246:1806 1:N:0:0 +TACGTAGGGAGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGCGTAGGTGGCCACATAAGTCCGTTGTGAAAGTCAAAGGCTCAACCTTTGAAAGCCGATGGATACTGTGTGGCTAGAGTACGGAATAGGCGAGTGGAATTCCTGGT ++ +@@@FDFFFGDHHHGIIJJJJHJJIIJJIIJJIJIIGIJJIHIJIGGGF>@BBDDDDDDDBCDDDEEDDDDDDDCCCCDDDDDDD@DDDCDBC@CDD>CCDAB.9BDDC(:>>>C:8?@@DDCCDC:?5?@BD@:45AA3(++34 +@M00176:17:000000000-A0CNA:1:1:16997:1807 1:N:0:0 +GCGCCGCCAAAACGTCGGCTACAGTAACTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCGTCGCGTCGTAACCCGGCTTGGTAAGTTGGATTAAGCACTCCGTGGACAGATTTGTCATTGG ++ +@@@?DADDHHHFF?2:?0)?BFDHABCDCCC8>A>(05<>B-&5>A(2(29<3+2(4>4>:::>A>+ +@M00176:17:000000000-A0CNA:1:1:18273:1807 1:N:0:0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCGAAACTGGCAGGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGG ++ +B@BDFFFFHHHGGJJIJJJJJJIJIJIJIJJJGHJIH'>ACBDDDACBCD&08CBDD>>:@C:>CDDBDBD79@D34:AAC(43. +@M00176:17:000000000-A0CNA:1:1:13837:1808 1:N:0:0 +CACTGATGCTGCTTCTGTTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAATTAACCGTCAGGATTGACACCCTCCCAT ++ +@@@DDD?DAHHHAGFHHFFHG=FCA=FH@HHIGCA@??F?@94:4:>>4:>@CA(+((44A(58&8993:4(>:@>(5?ACBCCCDB35>BD=>B5>BBDDACB:?7&+++95<8< +@M00176:17:000000000-A0CNA:1:1:12563:1809 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAGGCATTTGCTTAACAAATGTGTGCGAGGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFFHHHGJJJIJJIJJJJJJJJJJJJJIJEGHJJIJIGIIIJHIIIHHFDD-7B2A93>:C(4>::@>DC>@A8:@C3:>C>@>>CB:@::(+:<.&&00003:<@B55:@C::@CACCB>B&0>A>A9(+4::@C>CD +@M00176:17:000000000-A0CNA:1:1:13802:1809 1:N:0:0 +TCTTTCTGATTGTCCAGTTGCATTTTAGTAAGCTCTTTTTGATTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCATCAGCACCAGCACGCTCCCAAGCATTAAGCTCAGGAAATGCAGCAGCAAGATAATCACGAGAATCCTTTC ++ +CCCFFFFFDDH4CGIIIIIDHIIIIIIFHHEGBDFFHHFFFDFEECDBDBDDDDDD:@C@BDCDCDC?CB>AACCDDD(8<9C9:@CC:CCD +@M00176:17:000000000-A0CNA:1:1:17741:1809 1:N:0:0 +AAAATCGAAATCATCTTCGGTTAAATCCAAAACGGCAGAAGCCTGAATGAGCTTAATAGATGCCAAAGCGGTCTGGAAACGGACGGATTGTTCGGTAACGTGGCGCATGATTTCTGACCTATTATTGGTTGAACAGCATCGGACTAAGTTA ++ +@@@DDADDA=?CDB@1:?FE9FEH))09*0?=F9BB8@@=C3).=4))).777=;@@DB(38(&)&008AA>+:4(+++(((4:(4>A>8?C:(+919@A@9<0&(+((+4 +@M00176:17:000000000-A0CNA:1:1:16451:1810 1:N:0:0 +CAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAACAAAGAAACGCGGCACAGAATGTTTATAGGTCTGTTGAAC ++ +@@@FFFFFHHHHHGIGHIJIHHIIJJJJJJIEGHIJGH?DHGIIGIJJIJJJGGFHJJIJHIIGBEFDDD?C@BDBDDDBDBBBBDBCCDDDDDDDDDDDCDDD>@CDCDDDDDBDCDDD?BDDDBB?CC>>>@@34:>CCDDDD>C< +@M00176:17:000000000-A0CNA:1:1:15455:1810 1:N:0:0 +CGAATATCCTTAAGAGGTCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAACCATTTTTCGTCCCCTTCGGGGCGGTGGTCTATAGTGTTATTAATATCAAGTTGGGGGAGCACATTGTAGCATTGTG ++ +CCCFFFFFGHHDFGHFHBFHHIJJJJIGHHGHIJJJGIJIJAFFHIJJAHEHIHGHFFFFEEDDDDBDDEDDDABBDDDDEDCBABACD7@BBBDDACDCC::ACCDDDD< +@M00176:17:000000000-A0CNA:1:1:13595:1810 1:N:0:0 +CTTTTCAGAATCAGAATTAGCCGCAACTTCTGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACTCAAAAAGAGAGATGA ++ +?@1=ADDD?DDFDFE>@C@GII6DFGFIGH*:B?CGGGGIGIIIEIIDA@A@?CC??>@?B<@B:(8<5<05<.9>B>5CCCA8882@:>CCA>>:((9?<(4:3(315<9<0(4@4 +@M00176:17:000000000-A0CNA:1:1:17499:1811 1:N:0:0 +GCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTT ++ +@@@DDDDDHFHFFGGGFBBHIGA;F?DHIEHGCAGD44BD@BFEGCHFGHAEEGAHIIIIFFEEECCCCCCA@5>AC>@@=BBBCCCBCBBBBB<88?BCCBBCBBBB@.)89?(::4>>(>>C4@C33(+> +@M00176:17:000000000-A0CNA:1:1:18861:1812 1:N:0:0 +TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTAGGCGGTTGGTTAAGCGTGTTGTGAAATGTAGATGCTCAACATCTGACTTGCAGCGCGAACTGGCTGACTTGAGTACACACAACGTAGGCGGAATTCATGGT ++ +B@BDFFFFHHHHFCCFFHJIJJIJJJJJJJJJJJJGAFGIHHJJGIIIHIIIIJFDDDDBDDDDCDDDDDDDDDEDDDEEEEECDCDDDDDDDCDDDDDDDDDBBDDBDDDDDDDDBDDDDDDDCCCDDDDB?@>DBDADDDBCDBD??@CCC>A@+4+3:A>49:>@?@ACB9(2528?<>535<89:@:@:359. +@M00176:17:000000000-A0CNA:1:1:18411:1813 1:N:0:0 +TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAAGTTAAAGGCTGTGGCTCAACCATAGTTCGCTTTGGAAACTGTCAAACTTGAGTGCAGAAGGGGAGAGTGGAATTCCATGTG ++ +@@CFDDFFACBHAHIIIGHGBBFGIIIIIIIIIIIHB6@FFHII?EEDBCBBBBBBBCCAC@CCDCAAC@AC3@ACCCBCB<>?BACCCCBBC>CD+:8>.(:AC9:((+:::@:A@>>(+4:(+3?15.055@C>?3:>>CC:>C>C +@M00176:17:000000000-A0CNA:1:1:14902:1814 1:N:0:0 +CACGCGGCGGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAAC ++ +@@CDDDDDFHHDFICHFHIIIIJJJJHDEE2@7@BDDBDDDCCACDDDDDDEDDDDECDCDDCDDCDDDDDBD@BCD>>CDDDDDC@CDDDD<@CDDDD@DDACCAAA? +@M00176:17:000000000-A0CNA:1:1:18186:1814 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGGGGGTTATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGGGATCGCCTCCCTAGAGTTTATCAGGGGGTACTGGAATTCAATGGG ++ +@C@DDBFFHDDHGIGGIHIHHHIIJIGIJGIJJGIGB@FHGGHBCGHI;DGHDEE:5&0;&5&2::>(:>:>CAD@C429(4>:>>4+>>3><(4:(48A(:4+:2&290&()&5?<((9:>>>(4:>(4:(09004:@C+4:::4:4((+ +@M00176:17:000000000-A0CNA:1:1:13197:1814 1:N:0:0 +TCGCTACTTCCCAAGAATCTGTTCATAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTTAAGCAGA ++ +?@+ADDDDHHHHHGIAH?FHBHIII+AHGIIIIGHGIBC9>@BBB@ACABCEDC:>93 +@M00176:17:000000000-A0CNA:1:1:15519:1814 1:N:0:0 +CTACATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATT ++ +BCCDFFFFHHHDFGEHHIHJJJJJIJJB:CFHBGGIGIJGJJIJJJIJIIIGIJIJJJJJJIJJJEHIGHHHHHEFFFFFFEEEDCEEDDEDDDDDBDD9CDAA4:ACBDDCCDEDDD??::A:@C@:>CDDDCAC +@M00176:17:000000000-A0CNA:1:1:15059:1815 1:N:0:0 +AGTGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTCACCATAAACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCAATGGAGAAAGACGGAGAGCGCCAACGGCGGCCATC ++ +??=;DDEFHDHFFIGIEHIIIGIGHHIEII@EHEG;;6>CDC@C;@;CACCC@>>CCC3(8A(8<.59<ACBA>>9<395>@B.59@BBB&5.&))5?? +@M00176:17:000000000-A0CNA:1:1:14943:1815 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGGTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGAACGCCTCCCTAGAGGTAATCAGGGGGTACTGGGATTCAATGTG ++ +BCCDFFFFHHHHHJJJJHIIJJJJJIJIIJJJIIIIJHIIIHIJIJJJJIJJHHFDD8BD&8<8@:A3:B>CCACCD>A?CCC3@@A>@:3:AAC:3:?C::(+4(3>(+5<><ACD +@M00176:17:000000000-A0CNA:1:1:15097:1815 1:N:0:0 +GTGTGAATCATTAGCCTTGCGACCCTCGGCAGCAAGAACCATACGACCAATATCACGAAAATAGTCACGCAAAGCATTGGGATTATCATAAAACGCCTCTAATCGGTCGTCAGCCAACGTGAGAGTGCCAAAAACGATAAACCAACCATCA ++ +?@+ADDDDHHHHHIIIHI?FFIIIIHGA:DFHIIIIIIIIDHIIA?GIIFFEHHFEEECCCCCCDDDCBBBBBCCCCCCCBBCCCCCEECCCCCBBBBBCCCCC?B8<>B@B@CCBCBBBBB?CCCC((4:9@BBBBB@?:@32?BBBCC: +@M00176:17:000000000-A0CNA:1:1:19027:1815 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTAGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAAAACTGACTGACTAGAGTATGGTAGAGGGGGGTGGAATTTCCTGT ++ +B@@DFDEFHHFGDIIIIIJGGGIJIFHJJIIFHGIIJIGGFGAGGHIFFDDDDDDBDDDDDDDCDDDDCCDDDDDDDDDDBDDDBDDDDD8ABDDBCBCDDD>ACDD@C@:C?>CC>CD.)09@>@?3@@@CDCA> +@M00176:17:000000000-A0CNA:1:1:16207:1815 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAGTGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGCG ++ +CCCFFFFFHHHHHJJJJHIIIIJJJJJJJJJJJIIJJJJJJIJJJIJJJJJIGHFBD5CDCDDD(83>CC>@>CCD@:C9+::C<<>:+>:::9?CC@@:(>?BB&5:+4:+>:@C@CDC<< +@M00176:17:000000000-A0CNA:1:1:15387:1815 1:N:0:0 +ACGCTCCCAAGCATTAATCTCAGGAAATGCAGCAGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGA ++ +=?+ADDDDHHHHFIIII?FHHIIIIHIFFHGIIIIIIIIII?DFHGIIIIDGFHIIIIIGIGGHGIIEIHEECCBBCCCCCCCBB99>CCCCCBB?ACCCAAAADCCCC2<@BBBCCCCCC::?BABBC@CBABBBCCCCCC< +@M00176:17:000000000-A0CNA:1:1:16556:1815 1:N:0:0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGT ++ +BBCFFFFFDHHHHJIIHIIJIJIIJIJJJIJJHDIIG:AEFHIJJJHFFFD<>BBBBDDDDDEDEEEDCDDCCDDDDDDDBBBBBDDDCCDDDDDBCDDCCD@:>ACDDCCCDDDDDBDDC?34>ACDDBB@CBD.550BDB?C389>9<>>?89<5?>(3>CC:>(+4@CA:>ABB@B>?CC@CCC>C:@@( +@M00176:17:000000000-A0CNA:1:1:18322:1815 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATTCATTGTTAAAAGCATTTGCTTGACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGGACTGGAATTCAATGTT ++ +@CCFFDFFFHHHFEIIEHIHJIIJJJIJGIJJJJIJIADHHIJIBBFHHHIHHHF:B5&5(+3+4(:44:>C>:ACA:<344:3>(4(2:>@9((+(+8@:@((+4((+:(8909<8?3(4@C>:::3:>A109&0981234>:>:4+(+( +@M00176:17:000000000-A0CNA:1:1:16411:1816 1:N:0:0 +TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTAGGCGGATCTGTAAGTCAGTGGTGAAATCTCACAGCTTAACTGTGAAACTGCCATTGATACTGCAGGTCTTGAGTAAATTTGAAGTGGCTGGAATAAGTAGT ++ +CCCFFFFFHHHHFGIIJJJJJJJJJJJJJJJJJJJF;FHHHIIJHGIIHHHHHFDB@@CDDDEDEEC@CCDDDDCDDDDDDDDDDDDDDDDDCDEEDDCDDDCCCCADDD>ACCC(+4>ACCDCCC3>CCCE::>CCBDDBB3>CCCC>@( +@M00176:17:000000000-A0CNA:1:1:12878:1816 1:N:0:0 +GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAATGTGGCGGAATGCTCTTGGAACCAATTCACTTGAGTGAGACAGAGGAGAGTGGGATTTCGTGTGG ++ +@CCFDFFFDDBDDDD8AADADDDDDDBDCDDDDDD?BBDDDDDD<<@BACDDDDDB@<@CCDDDDDDDDCCACDEDACDB>B753<(:>@:CCCCDCBBB?BACCDA:(59?3)0:7A(((+ +@M00176:17:000000000-A0CNA:1:1:13982:1816 1:N:0:0 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTATATAAGACAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTTGTGACTGTATAGCTAGAGTACGGTAGAGGGGGATGGAATTCCGCGT ++ +BB@DDDFFFFBHFJJJJJJJJJJJJJJJJJJJJIIGIHIEEGIIHHGIIHHEDABDDDDDDCCCCCCDCDDEDEDDACCC@<9BDDDDDCBCB8?@CD>C>:>@@@(9>CA>ACDD:>@:>CCCC@?<38>@CBDDB95889@AAACC +@M00176:17:000000000-A0CNA:1:1:14848:1816 1:N:0:0 +TACGTAGGGGGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGGGTACGTAGGCGGTTTGTTAAGTTTGGCGTTAAATCACGGGGCTCAACCCCGTTCAGCGTGGAAAACTGGCAAACTTGAGTAGTAGAGGGGACAGTGGAATTCCTAGTG ++ +B@@F:BDFHFHHHJIJJJJFIIJIJI=CHHHFFFF;9ABDDCDD55>?CDD@37BBDDCAA>@BBBB>BD:?B9059<9>@>>4?4>>:>4>CC48.&&5<@>:9@(:(4++4:(4 +@M00176:17:000000000-A0CNA:1:1:15011:1817 1:N:0:0 +TAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATAACATCATGGTAACGCTGCATGAAGTAATCACGTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAA ++ +@CCDFFEFHHFHHHGHJIIJJGIIIGHDDBFHIGIGICHGFBGIGGGHGIIGGIIJJIJJIIHHEFFFFC@CCAACCDCEEDDD?B9?CDDCDDDCDDDDD:A@+4@CACC>CCCD?A@C?CDDBADBCDCCC:4>4:4>@@>3449:4>( +@M00176:17:000000000-A0CNA:1:1:18535:1817 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHHHHGDIIIJJJJJJJJJJJJJJJJJJJI:DHFHIJJJJJ@EGGFHFDD&07(2>AC483>CD:CCC@C@CCD@C@C>ACCD(4(43>9>ABB5@BDC(3:>(::>>CDCDAD959>@C:(:ACC<3((+4 +@M00176:17:000000000-A0CNA:1:1:17933:1817 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGGACTGGAATTCAATGTG ++ +CCCFFFFFHHHHHJIJIIIJJJJJIJJJJIJJJIIJI:FHHHIJIHGJEEHGGHFDD5<;8A??3>C>AD:AD:ACDC?9:CC:ACC>@>CDD:(4@:?CA@>:(4<39:?BDBDBDC><3:(4::(::>>?39&09A?13>:>:>A434@ +@M00176:17:000000000-A0CNA:1:1:18442:1817 1:N:0:0 +GCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAACCATTTTTCGTCCCCTTCGGGGCGGTGGTCTATAGTGTTATTAATATCAAGTTGGGGGAGCACATTGTAGCATTGTGCCAATTCATCCATTAAC ++ +?<@ADDFFFHHHFEHIHIIC@@EEFHIDHEG006?F@6;@FCH;75@CGHCCA?3?B<;=CCD@CEDD????<99?BB@BDD995>C@CDDDDA4>AACDCD:BD:3@CCCDBD@5>BBB?A@(+:>@A:(4@>>CC:>>:CCCDDCCC3( +@M00176:17:000000000-A0CNA:1:1:18207:1817 1:N:0:0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCGAAACTGGCAGGCTAGAGTCTTGTAGAGGGGGGGAGAATTCCAGGT ++ +@@CFDFFFAFHH3DDCBDDD503>3:ACDC<;)<@CDDDA>CDD@BDD>B1>ADDCBDDD?8CCDD@A+4:<<>C@9(3+95&05<@CCC>(3+ +@M00176:17:000000000-A0CNA:1:1:13068:1817 1:N:0:0 +AAGCGAACCAAACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATCAGCACCAACAGAAACAACCTGATTAGCGGCGTTGACAGATGTATCCATCTGAATGCAATGAAGAAAACCACCATTACCAGCATTAACCGTCAAACTATCAA ++ +:?+4=B?AFBHHFIIDEDFGIIFDDFF:?)9?FH<;;FHHIGIIIFHBEHEHE>@ECCCCBCCCCCBBBCBCCCEACBBB@BBBBCCCCCCD:BCCCCCCC@:CCCCCCC>>@:?CCCBB?BBCCCCCCA@?>:(:A:>8C=@FEHIIIIGGH:@FH))(0?BB@FGG@CDG(='5;8-53&2(++(((++(+:(4>4(((+4:((44(++43:(:43((+(:((+((+(+(+(+:+(3((((++43((((+(0&)&((+(((((((++4:: +@M00176:17:000000000-A0CNA:1:1:16588:1818 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGGAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGGGGGCATTGGAGATCGCCTCCCTAGAGGTAATCAGGGGGTACTGGAATTCAATGTG ++ +@<@DDDDDFFFFF1@?EG<;FF4@EBB@:BB4>AB3AA@@>@AB@>>(+(&05<4(+39+34(2509B73+<3((+(4>:::4+&5&)(:@@(::>A:(+(:@ +@M00176:17:000000000-A0CNA:1:1:14556:1818 1:N:0:0 +GACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTTGAGTCTTCT ++ +@@@FFDFDDFHHHIJACDDCDDDDD7CCCCBB@DBD::CCDDDBDBABBD?>ACDCCCCCC@>ACDC3>>AA::>>?BDB@BB?AA<:AC:@:>CC:: +@M00176:17:000000000-A0CNA:1:1:15755:1819 1:N:0:0 +CGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTT ++ +=@+=DDDDHHHFFIIIIGFGI@GIEHIIIIDIGIIIIIGFHAFG6AF/=AHE8BEEEC?@B:=CCCD>@@:ACCC(3>ACBC?0<@CCCCAA:A4::>B::@CC9@9<2<8<<@AB35?5>9?B<@&8?>>@C+(+8<<1243+4 +@M00176:17:000000000-A0CNA:1:1:12743:1819 1:N:0:0 +TCTGTTGAACACGACCATAAAACTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATAACATC ++ +B@CFFFFFHHHHHGHIJ+CHHIHIJ*?FHGGIEBFHIII?;BFHAFHIJIICGCH@EEFGHEFFBDBEEDCEDDBBDDDDDDDDDDDD<:@C:A@CDCD>CCDCD??BDDCCCDDDDD@C>(:@839334:>@CCCD<@C@CA +@M00176:17:000000000-A0CNA:1:1:15278:1820 1:N:0:0 +GCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGAAGAAGACTCAAAGCGAACCAAACAGGCAAAAAATTT ++ +?@+ADDDFGGF?FGEHHHHJGIJJJJFEGIIIJJFGIFHGII;CFGIHIJEDAHECEFFDB?@BDDBDDABDD>??<::A::@CCDDDDDACBCCB@59959(+8:@99>8>BDDD<>9CCDCCCDCCC3<>3&812((922(2((2&2:+ +@M00176:17:000000000-A0CNA:1:1:14225:1820 1:N:0:0 +ACATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAACTCATCACGAACGTCAGAAGCAGCCTTATGGCCGTCAACATACATATCACCATTATCGAACTCAACGCCCTGCATACGAAAAGACAGAATCTCTTCCA ++ +CCCFFEFFHGDHHJHHIJIJJJJJJJJJJJIIJJIIIIIHC?CGGHIIIJJICHIIJJJIJEGGGFHIIFEHGHGEFFFFFFFECCDBDDDDDD>CDDCDDCDDDDCCAC?B(??39>@AAACDCCCC +@M00176:17:000000000-A0CNA:1:1:13475:1820 1:N:0:0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTTGAAACTGGCAAGCTAGAGTCTCGTAGAGGGGGGTAGAATTCCAGGT ++ +@@BFFFFFHHHHHJJJJJJJJJIJJIJJJGIJJJIJJIJJIIJJIIHFFFDDDDDBDDDAC>CDEDDDCCDDDEEDDDDDDDDDDDBCDDDDDBDDDDDDDDDDACDCCCDDDDDABDDDDCCDDDCD????CBBD<@99?DDCDDDDDB+ +@M00176:17:000000000-A0CNA:1:1:17441:1820 1:N:0:0 +GAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAATGGGACGAAAAATGGTTTTTATAGAACGAGAAGACGGTTACGCAGTTTT ++ +@@@BDDD??FFFB,>B<>(8B>::CCCD:@C3>CD@A<:@C@>@C:>CCCBCC@DDDD4:>(+499>(2?B>B>CCA:4>105)0:4+:((+:AA((4>C +@M00176:17:000000000-A0CNA:1:1:13904:1821 1:N:0:0 +TACAAGTAAGACTAGTGTTATTCATCTTTATTAGGTTTAAAGGGTACCTAGACAGTATATCTAGCCTCAAAAGGGAACAGATTTACTAGAGTTTTATGTGAGAGGAAAATATTAGAACCATTGGAGTAGTGATAGAATATTTTGATACTAT ++ +BBBDBFDFHHFHHIIFHJIJJJJGJJIIJJJJHIIHHJJIIIJJIJJIIJJHIGHGIIIIJJJIJFHIJIGEGIJJIIGHHGHFHCEDFFCCEEEDEEEFCCCC@??A?CCDDD>>>C@?CACC@CDDCCCDD:(:::>@CBA@8ADCD:( +@M00176:17:000000000-A0CNA:1:1:14166:1821 1:N:0:0 +ATCAATACCATGAAAAATATCAACCACACCAGAAGCAGCATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCGCCAATATGAGAAGAGCCATACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGA ++ +@@CFFFFFHHDDHIJJJEEIHGIIIHIIIJCH>DFIIIIII@HGDFHIFGGEA8@GG@:FEGGDHGHIGFEFB?C@C:A@ACCB(4>@CBDBBBDDCCD33::>3(4:>AACAA@AACD& +@M00176:17:000000000-A0CNA:1:1:12519:1822 1:N:0:0 +AACATAGGGGGCAAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGCAGGCGGTCTGTTAAGTCAGATGTGAAAGGTTAGGGCTCAACCCTGAACGTGCATCTGATACTGGCAGACTTGAGTATGGAAGAGGTAAGTGGAATTCCTAGG ++ +@@CDDFFFFAHGHJJIIJJEHIJGIJIIEEFGIIIHIFHIHGFH?C@A:A@::?AB8?>CC:>>A(:?>@A8(>>A@:@C::>@>C( +@M00176:17:000000000-A0CNA:1:1:13303:1822 1:N:0:0 +TACGTAGGGGGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCCGCAGGTGGCAGTGTAAGTCTGCTGTCAAAGGTCAGGGCTCAACTCTGAACAGGCAGTGGAAACTACACAGCTAGAGTGCGGTGGGGGCAGAGGGAATTCCCAGT ++ +BBCFFFFFGHHHHJJJJJJJJJJJJJIJJJJJIJIHIIIJJJIHHFFFDDDDDDDDDDDDCCDDEEEDDDDDDDEDDC>ACDDDDDBDDDDCDDCDDCDDDDDCCDDD@ACCCCDCDDDAACCC>CBBB>.09+ +@M00176:17:000000000-A0CNA:1:1:16634:1822 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCAAGCTAGAGTATGGTAGAGGGTGGTGGAATTTCCTGT ++ +CCCFFFFFHHHGHGIHJJJJIJJJJIJJJJIJJJJGBGHHHIJGGIJFFDDB5@BDDDDDDDDCDDCDDDDDDECDDDDDD@>@BDBCDCBCDDBB?<@CDC@:3>A@8?CCCDCA>A>?4:@ACBD8?302C:A::A> +@M00176:17:000000000-A0CNA:1:1:18685:1823 1:N:0:0 +TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTAATAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGTTAAACTTGAGTGCAGGAGAGAAAAGTGGAATTCCTAGG ++ +@@@FFFFFHHHGHFIIAHIHIIAHHHIIIIIIFF=BD(?FFFGHEHHFCBBBBCBBBCCCCCCCDDDCCDCDC>CCCCCCCBBCCCBCCCAB@BB??8>BC?CE@CCACCCCCAC:C@CCCCC>:@CC1+81<0>>99@@CC@CACCCCC3 +@M00176:17:000000000-A0CNA:1:1:17794:1823 1:N:0:0 +GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGATTCGGGTTGAAAGTGAAAGTCTCCAAAAAGAGGCGGAATGGGCGGGGAAACAATTCACTTGGAGGAGACAGCTGGGAGGGTGGATTCTCGGTG ++ +@@@DD>DD@1@BB?B38AC8@CABBBBBCCCCCBBB&00?AC@?(8?:ACBBC8B702(:30<@??B(:(:ACC@A:@C(((4(+28&(387595)(4(((&&)&&&++3(((((+(++((+((+((((((+(((()&((&(+4(((+++( +@M00176:17:000000000-A0CNA:1:1:16040:1823 1:N:0:0 +TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGTGGTTTATTAAGTCTGGTGTAAAAGGCAGTGGCTCAACCATTGTATGCATTGGAAACTGGGAGACTTGAGTGCAGGAGAGGAGAGTGGAATTCCATGTT ++ +?@CBBBBBBB:A@BDBEEE394??BDEBB?@2(3>?B(4:4AA(44(::A@ABB<(+29?B3>3>4(+4>1(+(882(2<:>?@>3>A>@(+(( +@M00176:17:000000000-A0CNA:1:1:17639:1823 1:N:0:0 +TCTTGTTGACATTTTAAAAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCG ++ +CCCFFFFFHHHHHIJFIJJJJIJJIIIIJIJJIEDCHD>?FGHIBHGDG9FGCHIJIIJIGHHIIEGHEHEAHHFFFFFFCEEEEEEDDCCDCDDCDCDB@BBBDDDDDDD@C@CCC>A::@CCCDCAC::9<@AAC@@CD:134:>@:@C@C(>ADAA4:>>@9<9CC>?A>@BDDBDDB3:<((+:>CDD@CC<9>&09?> +@M00176:17:000000000-A0CNA:1:1:14698:1824 1:N:0:0 +TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCAGGCGGTGACTTAAGTGAGGTGTGAAAGCCCCGGGCTTAACCTGGGAATTGCATTTCATACTGGGTCGCTAGAGTACTTTAGGGAGGGGTAGAATTCCACGT ++ +@@CDDFDFAHABFGGIIIIIBFIIIIGEGGHIBHG=AAB;@AHH3>>(3&)05<90>A@CA(448? +@M00176:17:000000000-A0CNA:1:1:17135:1824 1:N:0:0 +TACGGAGGGAGCTAGCGTTGTTCGGAGTTACTGGGCGTAAAGCGCGCGTAGGCGGTTACTCAAGTCAGAGGTGAAAGCCCGGGGCTCAACCCCGGAACTGCCTTTGAAACTAGGTGACTAGAATCTTGGAGAGGTCAGTGGAATTCCGAGT ++ +@?@FFDFFHFHGHGIIJJJJJJJJJIJJJJJJJJIIHEGIIJIJDFDDBDDBDDD8BBCCDDACDDEDACDDDACCCDDDDBDDDDDDDDDDBBDD<@CCDDCDDDC>CCDC@>:@CC@CDCCACCDD>A?>?@ACCDDDDDA>ACCC&&+ +@M00176:17:000000000-A0CNA:1:1:15983:1824 1:N:0:0 +TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGT ++ +B@@DFFFFHHHHHJJIJJJJJJJJIIJ=GGIIJJJIJIHIIEGIIBHGHJJIEIHHHHHFFFFFEDEDDBDBBDBDDDCCDDDDB8ACCDBB@DBAA@@BDDD<>>BDDCC::CCDDBCDD?C@CCDBDBB@89.5>08?>3<1>CAAB>) +@M00176:17:000000000-A0CNA:1:1:16291:1825 1:N:0:0 +GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCGGGAGTACGGTAGGGGCAGAGGGAATTTCCGGT ++ +?1=BDDFFFHFFHIIFAFGEHEGIIGHGIEIACBBBCCBB>@??53)5>BC=9>:@C:CB?(9>BB?ACB99A.8?B93.2<(59<<>(+4<&& +@M00176:17:000000000-A0CNA:1:1:18597:1825 1:N:0:0 +TTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGA ++ +BCCFFFFFHHDDHJJJJJGIJIJFIJJJJII;?EHBG:@GHGIHGHIGCHGGGJEGGIJGGII@GCHIHCCEH@DECDDBDDDCCDDCCCDDDDDDDDCDDCCCACCCDCDCDDDDAABDDCCC:@ACDDD?3>@@C<<@3(:@>ACAC@4 +@M00176:17:000000000-A0CNA:1:1:17047:1826 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGGACTGGGATTCAATGTG ++ +CCCDFFFFHHHHDGIIHHJHIJIJJIJJJIIJJJJIIBHJIIJIGJIIIIJJGGFD@-7-<<3(+>C4>BCDC:CDD>A9:@C@CCDACCCC?344>48>@>C(+(+9>4825&9?BC:<((+3(+(:(((29>&&0?C(@A>(( +@M00176:17:000000000-A0CNA:1:1:16807:1827 1:N:0:0 +AGCCAACGTGAGAGTGTCAAAAACGATAAACCAACCATCAGCATGAGCCTGTCGCATTGCATTCATCAAACGCTGAATAGCAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCCAGCAATCTTGAACACTCATCCTTAATACCTTTCTTT ++ +@@@DDDDDHHBFC<222AFGIGGAHIGCHGIIBEGG000909B?@ABDGII8CFCGIIE;=CEE??AB3?BA?C@B@CCCC>A>>?BCCCCC05>9@??CCD3(+4+4<8??C?BCCA9<((4>:4:@CC49?(:CCCC3:@@>CAAAC:A +@M00176:17:000000000-A0CNA:1:1:13102:1827 1:N:0:0 +AACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTTGATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCC ++ +@CCFDDDFHHHHHJJJGHIICF@GDGHHHGIGHGHFJHIIGHIHJJGHCEGIJJJEEGGFHJCHCEGIJGFBHF>BCCCBBBDACDD:>8?B?CCCCC>ACC@A3(44:>09ACC@CC:43:@9:(:><8( +@M00176:17:000000000-A0CNA:1:1:19117:1827 1:N:0:0 +TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGGTTTTTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCGTTGGAAACTGGAAAACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGT ++ +BBCFFFFFHHHHHJJJJJJIIHJIJFHIJJJJJJJJJJJJIHIJDIHFFDDDCBD@BDDDDAACDEED@CCEDDDDDDDDDDDBDDBDCDDB8?@(8@>9CBCC:?@CC9C@9>CDCCAACCCC@?>>ADBB>?+4:A:AACDA:A: +@M00176:17:000000000-A0CNA:1:1:18782:1827 1:N:0:0 +GTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGTCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCA ++ +@@@DD;D=FDFHHI@@C?EE<3?C:C@CCB@=8?B37<:AA(+>>A>>(:3>:4::>:ADCC@CADC<:@CCC>?CC@:@>AC?:33<1<80<8?(:@34@A4>@CA>3(:C>:ACC:( +@M00176:17:000000000-A0CNA:1:1:13786:1828 1:N:0:0 +ATAGTGTTATTAATATCAAGTTGGGGGAGCACATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAACTCATCACGAACGTCAGAAGCAGCCTTATGGCCGTCAACATACATATCACCATTATCGAACTCAACGC ++ +@@@DDAEFFFHBFFEDGIJJHID@GID:F@DFDFDHI4BGIGGDFHHHGEIEGFFFIFIIJ>EEHGCEEHACH:?BFCDCA:AAD(5>@ACBDDDDBB?9AC9A>?B@CCACC:?>>0525ACD>@>@CAACCCCC>AC<(2(2ACCECDCDDA@C@CCDADD@>B>AA:CCAC>A>CCC@B +@M00176:17:000000000-A0CNA:1:1:16604:1828 1:N:0:0 +ACAGTCGGGAGAGGAGTGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTCACCATAAACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCAATGGAGAAAGACGGTGAGCGC ++ +@>@B?<>:>@C:2,;4:@>8:::>B@A(((+4@:@:@C@CCAC9<22((05<<.&50>@3(((+49<><&+2+(9> +@M00176:17:000000000-A0CNA:1:1:18303:1829 1:N:0:0 +GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAAAGTGGCGGAATGCTCTCGAAACCAATTCACTTGAGTGAGACAGAGGAGAGTGGAATTTCGTGTGG ++ +@@@FFFFFDDDB@8?8CDDDEDDDDDDDDDDDDDD@0-6?ADDDDDDD8ABDD8B5@@3:@BDDBDDDCCD:@CDDDCCDBDDDD??@A@CBBB>97@CC:@C5(385AB@ACC@@:4>3:+4((4:3<9>88?2+(4<:43:::3(+2(( +@M00176:17:000000000-A0CNA:1:1:14128:1829 1:N:0:0 +TACGAGAGGGGTAAGCATTATTCATCATTAATGGGTCTAAAGGGTACGTAGGCTGTATCATAAGACTGAAAGGTATCGAACTAAATGAATAAGAGCTTTTCAGTAACTATGAAACTAGAGTGTAATGGAGGAAAGAGGAACTCCGAGAGTA ++ +BBCFDFFFHHGHHJIJJIJGIJIGIIJIJJGIIJIDGIJJJIJJHEHIHIIJJGGHIJJJJIJJIHHHHHFFF;BEEDBCBDCCACC@CDEDCCCCCDDDDDCC@CDDCDCACCDCCDDCCDDDDDDCC8<@BABCCBDD?CC>@9<9<+( +@M00176:17:000000000-A0CNA:1:1:15205:1829 1:N:0:0 +TGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTG ++ +@B@FDFFFHHHHHIIEHIGIHIGIIJJIJGIJGIGI??DGFEIJIJJJIJIJJJJJGHIGJJGHIIJJHHHHFHFFFECD@CDD?AD@CCDC@CDDCDDEDDACDCCCDEEDDDDEDCCDCDDDDA<29?BCDDD?AACCD +@M00176:17:000000000-A0CNA:1:1:13744:1829 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGTGTTAATTAGGGGGTACTGGAATTCAATGTG ++ +@CCDFFFFFAAFDIJJEGHFHIIIIIHIH@GIDDHHGIJIIHGCDGIIIJJJHHFB<&558A?>@CA:>B@CC:AAC:?9CCA>:@A:>@>C?CA>3+2A(:43((993(209&04:>::(((4<5<>&4>>C(:>>>@@CC:4 +@M00176:17:000000000-A0CNA:1:1:18973:1829 1:N:0:0 +TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTAGGCGGATTTGTAAGTCAGTGGTGAAATCTCACAGCTTAACTGTGAAACTGCCATTGATACTGCAGGGCTTGAGTAAATTTGAAGTGGCTGGAATAAGTAGG ++ +CCCFFFFFHHHGGJJJIJJIJJJJJGIIJIJJGIJFGHIJIJGEHGHEHIHDFFD>BDDCDDDDEEDDDDDDDCDDDDCC>AA@?CACDDCDADEDCA@CDDACDDC@>CDDDACB&8AB@8@>>>(>CCCD(:4:>?@BBBDDDDDDDD>@DDDDACD>@ACCCDDDC(8><@@DCC<3:@(4>>>>988(8<@CCD=39CC@:>C( +@M00176:17:000000000-A0CNA:1:1:15612:1830 1:N:0:0 +TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTAGGTGGTTTGTCGCGTCGTCTGTGAAATTCCGGGGCTTAACTCCGGGCGTGCAGGCGATACGGGCATAACTTGAGTACTGTAGGGGTAACTGGAATTCCTGG ++ +B@CDDDEFHHHHGIJJGHJIHHIJJIJ@GIIJIIJIJIIJJJIGHHHFFDDDED?BDDDDC@DDDDDDDDDCCDDCCDDDDDDBB@ACCCCCADB@B<<@DDC?BDDBDBBBBBBDADCDEDCC>:>@>:>CCB399>>CC3>(+:@CAC? +@M00176:17:000000000-A0CNA:1:1:19230:1831 1:N:0:0 +NACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAANGNGNACGTNNGNGGCTTTGTAAGTTANAGGTNAAAGCCNGGAGCTTAACTCCAGAACTGNNTTTNANACTGCNNNNCNNGNANCNAGGAGAGGTGAGTGGAATTCCGAGG ++ +#1=DDFFFAH@FAIIJGIJEIJJJJJJJJJIJJJJJJHHF#,#,#,,;?##,#,5##++(#+#+2<8<####+##(#+#+#+++2<(8?A1(8@A?A@CDCCA&5& +@M00176:17:000000000-A0CNA:1:1:14582:1831 1:N:0:0 +TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGTTACTCAAGTCAGAGGTGAAAGCCCGGGGCTCAACCCCGGAACTGCCTTTGAAACTAGGTAACTAGAATCCTGGAGAGGTGAGTGGATTTCCGCGG ++ +CCCFFFFFHFHHHIJJJJJJJIIIJJJIIJJJJIIGGHIIFJIIIGDFECEDCDDDDDDDCCDDEDDD>C@CBCCCCDDDB>BB<BBDBCDCCDDDD(:>@:?&0& +@M00176:17:000000000-A0CNA:1:1:18818:1831 1:N:0:0 +TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGCGTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCATTCGAAACTGGCATGCTGGAGTCTTGTAGAGGGGGGGAGAATTCCAGGG ++ +?@@B7DDFHHHHAFIFIIIIIIIIII;GIIIIIIIIGAHIHIIGGBB:<@BCCCBC@A@C>>8>@C>>>@C19059&5>>BCCCC@C?& +@M00176:17:000000000-A0CNA:1:1:13507:1832 1:N:0:0 +GCTCTCGTGCTCGTCGCTTCGTTGATTCTTGCGTTTATGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTCCTGTTGATTTTATTGCTGCCGTCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCAT ++ +@@@DDDDBFH?DFFHEFG9FEGHB;;?@@B9;?B@CAC>@A>((4>BCCCACCCB@<0(8>A@:>>C:(+:@CDDDD:???@<&:@3::3(4@980<&(+>@AC4@:: +@M00176:17:000000000-A0CNA:1:1:15881:1832 1:N:0:0 +CAGCACGCTCCCAAGCATTAAGCTCAGGAAATGCAGCAGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTC ++ +?@+=DEFFHHHHHIIIIIIII>551>A?:>:@(:::>A?>55@@(3<9B5>>>>3:550)05<53++:> +@M00176:17:000000000-A0CNA:1:1:12270:1832 1:N:0:0 +GCACGTAATTTTTGACGCACGTTTTCTTCTGCGTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGGCTTTAACCGGACGCTCGACGCCATTAATAATGTTTTCCGTAAATTCAGCGCCTTCCA ++ +@CCDFDDFHHHHHHIJJJIJJJJJJJJJJJ@DHHGFHHIJDHHJGIIFGHG=@DHIJHHHFDDDDDDDDDDDD:CCDDDDDDBDDCCDDCC@BBBDDDACDDD@<@BBDBDBBDD55?:@::ACDCCDDDA:?>AB?@>@CCD3<595C?( +@M00176:17:000000000-A0CNA:1:1:14447:1833 1:N:0:0 +GACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGAGTGTAGGTGGTTGTCGTAGTCAGGCGTGAAAGCCTTGAGCTCAACTCAAGAAATGCGCTTGATACTGGGCAGCTAGAGGACCGGAGAGGATAGTGGAATTCCCAGG ++ +@@CFDFFFHHGHHIJGIJJJGIJJJGIIHIJIJJIFGEEHHGHHE>,6>CA;@@@DDB8@BACCCCC:>CEBCCCC(+:4>( +@M00176:17:000000000-A0CNA:1:1:12871:1833 1:N:0:0 +AACACAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGTCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +@@@DDDFFFFDFFIJIGGIAH?HHJGGJJJJJJJJJJJJIIJIJBHIJIIJGEHDDD8@;(2CDC::CC@BCCC>>@ACC@>>:ACC>@>A@ADAC@:AAD>8(2(3:>C+(+:@:4:3((:@15<&)(+>A>::>@(:@:>4 +@M00176:17:000000000-A0CNA:1:1:18056:1834 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +B@CDDDFFHHHHHDIGIHIGIJJJJJJJJIIJJJJIJGIIFFHHGEGHGIIIHHFDD&5<2<3934A:@CCDD:ACD(13>CCDCDDCC@>CB3:@A>AA:C:::+33>(2?>@D??C:3(4::@A>(4::ABD>9C@C:9>ACC:@::(+ +@M00176:17:000000000-A0CNA:1:1:17984:1834 1:N:0:0 +AGATTTGGAGGCATGAAAACATACAATTGGGAGGGTGTCAATCCTGACGGTTATTTCCTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTGTTCCAATTATCGGCAACAGCTTTATCAATACCATGAAAAATAT ++ +B@BDFFFFHFHHHIJFIJJGIJGJIJIJGIFFHEH:6?FGIIJIJJJIJ@FC;@EEGGGIEHFH?=?DFC@DCBECECCDC>CC@ACC>CDDD19ABDDDDC>3(+4:4>CDD:(+4>CA>@9@B?BAC3>A>AC>CCC>@CA>>((28(( +@M00176:17:000000000-A0CNA:1:1:15416:1834 1:N:0:0 +TACGTAGGGTGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGTAGGCGGTTTGTTAAGTCTGATGTGAAAGCCCGCGGCTTAACCCAGGAACTGCAGTGGAAACTGGCTGACTTGAGTGCTGTAGAGGAGAGTGGAATTCCTTGT ++ +???B=DDD72CDBAA3+:BAEEAA3?>;AAA(((+<<<>AAA3++4>(4:9+:((3829A3:>AAA>3:+(:3>>>?>(((8AA:>>A:(4+ +@M00176:17:000000000-A0CNA:1:1:15709:1834 1:N:0:0 +TACAAGTAAGACTAGTGTTATTCATCTTTATTAGGTTTAAAGGGTACCTAGACAGTATTTCTAGCCTCAAAAGGGAACAGACTTACTAGAGTTTTATGTGAGAGGAAAATATTAGAACCATTGGAGTAGAGATAAAATATTTTGATACTGA ++ +??@DD>:B:?:ABDDHIIGEHEHIGBFAEHGHAFC9*:CFHGIDA@BBGI@BDGDEGGDBCGIII>DC?D@@GHCE?3@BD;7.;A;:>;>@;@CCAC(95((,5>>CCCCC@@@>CC@343:3>>:4:>AAC@>B3:(:@:C3+ +@M00176:17:000000000-A0CNA:1:1:15154:1834 1:N:0:0 +ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTC ++ +@CCFFFDFHHGDHJJJIJJIJJJIJJJJGIGHG???FGHIIIIJH@DDHHIHIHJIJJCGIJIJIIIGIJHHGHDBDFDFBCDDB5@CCCCDDDDEECCC@CACDEEC>ABCDC>ACB@BCDD@:CCACDD>9.>@CEEDC>FGGGEBEE=;752>@>ADD>ADCACACCD>>:@>:@:?9@B>&2@CC@A8A44:@34(:>AC@<@C((:>(:4:ACC3+4(+(+2(<.5+3(:(4:@:449: +@M00176:17:000000000-A0CNA:1:1:16996:1835 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGTGGTTGATCCATTGTTAAAAGCGTTTTCTTAACAAATGTTGTCATTGGGAATTGCTTCCCTAGTTTGAATCTGGGGGTACTGGGATTCAATGTG ++ +@@@AD?BDF?FF>EGF8CHFHEFI6CGGICGIDFIF>FFDFFI288BFFFF=EE<')&&&)8((+4((,:4@((+>B:(&+8@((+>@:(+:2>4(((+(+(44>((+((4(44(493+(+(+(+(4>3>:35>&0>AA:(8>3>(4@3(+ +@M00176:17:000000000-A0CNA:1:1:15945:1836 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGGTTATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHGHHHJJJIJIIJJJJJJJJJJJJJJGJJFHDHGGJIJIJJIJHHHE@B-7@5@(8@@A34B@CDCDDD:<>>>@@CCC@C44>A@:4@CAA(:>::++(+>(<<<>BB<3(+:(+:@A@:@><<@@<>CC39>>3@@@D:>C +@M00176:17:000000000-A0CNA:1:1:15829:1836 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAAAACTGACTGACTAGAGTATGGTAGAGGGTGGGGGAATTTCCTGT ++ +BBCDFFFFHHHGHJJJJJJJJIJJJIJGJJJFHGGGI8?FFHEIJIIFBCDDDDDDDDDACDEDEEDDDDDDDEEDDDDCDDDBD>?CCDDDDDBDDDDDDD4>CCDCCDCCCDDC:ACA>>:4:ACDDDDDDD9<<@&09098:4>CC@> +@M00176:17:000000000-A0CNA:1:1:18386:1836 1:N:0:0 +AAAGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAATCGAAATCATCTTCGGTTAAATCCAAAACGGCAGAAGCCTGA ++ +@@CDDDFFHFACFHIJJJIJJGFHIJIJJHGIJIIJ000/07B<@DCDACC:ADDDAC??C?9 +@M00176:17:000000000-A0CNA:1:1:12988:1836 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGGGGGTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGGGTTAATCAGGGGGTACTGGAATTCACTGTG ++ +@@@DDDDFHHHHHHHBDGIHIIGHIGHIJJIIIIGGIDHHGGDGIIIJCDG=>BB=@&00&572>>>4+:4:@::>CC13>@AD(4>4>:3:<::(4+8>:@@A:4?C(:87905???(((+3<8>@(4>CAB<5<@>:A3>@C:@(4:AC +@M00176:17:000000000-A0CNA:1:1:15728:1836 1:N:0:0 +CATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGTTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGTTTGTTTCTGTTGGAGCTGATATTGCTTTTGATGCCGACCCTAAATTTTT ++ +@@@?DDD?FFFHHIIEHFHI<@FHG?FHG@HFE)1?9BFG>FHBGDFE*/9BFHHIHIIIIIGIHGEGIIHEHFDCEDECCCDC(6;@;3>;3=B>5A>@C((+:2@>CC1(+3(+4:A55<35AC3:>>CCC +@M00176:17:000000000-A0CNA:1:1:13129:1837 1:N:0:0 +GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAAAGTGGCGGAATGCTCTCGAAACCAATTCACTTGAGGGAGACAGAGGAGAGGGGAATTTCGTGTGG ++ +CCCFFFFFDDDDBDB8@AD@DDDDDDDDDDDDDDDADDDDDDDDBBDDDDDDDDDBB@CDCBDDDDDDDDDDDDECD>@BDBDBC@A<>CC?@B.0?A:>44(0(9<98ABCCD3(::C3+(()00893(38(828(5<&08>4+9A@88( +@M00176:17:000000000-A0CNA:1:1:18507:1837 1:N:0:0 +TACAGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTTAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGT ++ +BBCFFFFFGHFHHGJJBGHIIIJJIHEIJJIJIIIGIBHIFHHI;FBH@DGG@EHHHHHDFEFFEFEDDDDB?BDDDDDDDC@BB?BCDDD@BC8CCCBBDC3>@BCBB??599<:@?.) +@M00176:17:000000000-A0CNA:1:1:14782:1837 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHHGHGJJJIIJJJJJJJJJJJJJJJIJJJDGHHGGGIGIJHHJJHHFDD5;B8ACCDAC3@CADC@CCDC?C@CC@CCD:@::AA@CCCCDACC(4:@CCCD?@DBBDB9>C@C4:3@A@C>@(5<09CDCACCDCCCC::(( +@M00176:17:000000000-A0CNA:1:1:15801:1837 1:N:0:0 +TACGTATGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGACGGTTGATTAAGTCTGATGTGAAAGCCCGGAGCTCAACTCCGGAAAGGCATTGGAAACTGGTCAACTTGAGTGCAGTAGAGGTAATGGGAACTCCATGT ++ +BBCFFFFFHHHGFIJJIJJJJJJJJIGIJJJIJJJJJGGHEGIIJJHHDEDABBDDDDCDDCDDDDEDDEDECEEDDCDDDBDDDADDDDDDDB@9BDBBBDC@@CCCDDDDDDCC:CC@:3444:CC>ACCC1(+:((+2(98>:443@+ +@M00176:17:000000000-A0CNA:1:1:16705:1837 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTCGATCCATTGTTAAAAGCGTTTGCTTAACAAATGTGTGCAATGGAGATCGCCCCCCTAGAGTTAATCAGGGGGTACTGGAATTCAATGTG ++ +@CCDDFFFHHHH@FHIHHIHJJJJJJJIJJJJJIJJ6@FHGGHHCGIH;@EHHHF@B/7B8AB@88?3@C3@@ADCC38<(29@CC4(:@>CB>(+4>AC:(4>CA1(932<5&0<@<2<::>C:>>:@(:80959ACCDCCCACD44@AC +@M00176:17:000000000-A0CNA:1:1:13088:1837 1:N:0:0 +TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCAGGCGGCCGAGTAAGTCGAATGTGAAAGCCCCCGGCTCAACTGGGGAACGTCATTCGATACTGTTCGGCTCGAAGATAGGAGAGGGAAGCGGAATTTCAGGT ++ +?@@DADEFHFGFGIIEGGIGGGIGBAHGGGIIIIDIIFFGGGIIIGEGFFDDDBBDDD;B?ADDDDBBDCDDE@DC@ABDDD9>BBDAAC:?C?9@BDDD?@BD::>@B@C@:>>809295.(8&:(:>:?(885<202@CCCA@A: +@M00176:17:000000000-A0CNA:1:1:17186:1837 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCTTTGTTAAAAGCATTTGCTTAACAAATGTGTTCATTGGGGAACGCCTCCCCTGAGTTAATCAGGGGGGACTGGAATTCCATGTG ++ +@@CBDFFFDDBFHGIIGHIJJJIIJIJJJIJJIGGGGGIJGGG>CHHIGG>(44(:34>CC(<33+::ACC@AC::8(44>:(4(+:>((&+&2&)0(088&((((+::@D:(:@83>.9918?((+4+(+(+(+ +@M00176:17:000000000-A0CNA:1:1:17204:1838 1:N:0:0 +TACGGGGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCAAGGTAAGTCAGCGGTGAAAGACCAGGGCTCAACTCTGGAAGTGCCGTTGATACTGTCTGGCTAGAATGATTCCGCCGTGGTAGGAATGAGTAGT ++ +B@BFFFFFGHHGFGGIJJJJJJJJJJJJJJHHHHHDB?CCACED;?BB?BBDABDDBDBDDDDDEEADC>BDBDDDDDCD?BDDDDDDCCCCCDDDCDCACCBB&8AC:@:B>><809@@8(+8A3:>>C@:C+ +@M00176:17:000000000-A0CNA:1:1:16197:1838 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGAATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCAAGCTAGAGTATGGTAGAGGGTAGTGGAATTTCCTGT ++ +BBBFFFFFHHHHGJJJIJJJIJJJJIJIJJIHIIFIJJIJIIIIGIIEDCDDDDD?@BD9?3>ADEDDDDDDDDDCDDDDD5>BDDDDCCACBBDBDDDCCDDCCD@CABCAC@D(2?CACCCCCCCCCA:4>?@CCC@>CCC+ +@M00176:17:000000000-A0CNA:1:1:14193:1838 1:N:0:0 +TACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGTGTAGGCGGCTCGATAAGTCTGCTGTGAAAGCCCAGGGCTCAACCGTGGGATGCCGGTGGAAACTGTTGGGCTCGAGTCCGGAAGAGGAAGGTGGAATTCCTGGT ++ +BBCFDFFF=FHHGJJJIJJIIJJJJJJE3BGIHIJDIGHIIJ):EF';';AA3=B&&&)5??BDEE3@(:CDDDEDC(8AA(8>BBDDDCB&2<<&((28@.&0008(4>CCC34(&2<(9<>4(:+935018(2(+2?CDBCDDCC:>C4 +@M00176:17:000000000-A0CNA:1:1:12840:1838 1:N:0:0 +GACGGGGGGGGCAAGTGTTCTCCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAAAGTGGCGGAATGCTCTCGAAACCAATTCACTTGAGTGGGACAGAGGAGAGGGGAATTTCGTGTGG ++ +@@@FFFFFDDDDDDD@CDEAD3@BDDDDD@CDDDDBDDDDDDDDBDDBACDDDDDDDBCDDDDDABDA@:CCDCDDCCDDDDDBC??9>CDDDB9>BCACCCC<(<@B1:CAC@CACD@12<((3(+812(&0&&+::>::?CDDDDB@CCC9A>>>@C@CDCBCBBCC1+8ACDDEDE<> +@M00176:17:000000000-A0CNA:1:1:12956:1839 1:N:0:0 +TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTCTGTTAAGTTGAATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCAAGCTAGAGTATGGTAGAGGGTAGTGGAATTTCCTGT ++ +BB@FFFFFHHHHHJJJIJJJJJJJJIJJJJJJJGGHGHIJIJFIJJIFFDDDDDDDCDDDDCDDEEDDDDDDEEEDDDDABBDDDDDDDDDDDDDBDDDCCD:>9@CD@DDDDBC>@?CDDCCCDDED@>ACCBB8?B@?BDCCD>@4>C( +@M00176:17:000000000-A0CNA:1:1:18356:1839 1:N:0:0 +TACGTAGGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCGTGTAGGCGGTTCGGTAAGTCTGCTGTGAAATCCTGGGGCTCAACCCTGGGCGTGCAGCGGATACTGCCGGGCTAGAGGTGGGTAGAGGCGAGTGGAATTCCGAGT ++ +@@@DDDFDBHHCFGGBBCACCB8=8<>AA56BBBBB??@CBCDCCCCCCDA>AACCCCBCCCBCCCC@BCC@?9&)0<((0&559CADC>@1<>.5>BB>>@AA(3&&+ +@M00176:17:000000000-A0CNA:1:1:19008:1839 1:N:0:0 +GTTTACCTTGCTTGTACGCGCATGAAACACTGACGTTCTTACTGACGCAGAATAAAACGTGCGTCAAAAATTACGAGCAGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGTCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAG ++ +@?@DDDA??:A22+<<<:@@@??D?BDD3?6?B(7?FFFF>)554'')))).7;B6;(9,,39(59(>>:((05-3339?2838@@:B:(+:(:>34::3((++4>&)02(+(4((28.<00&)52?>()&++9.995(&0&+00&5 +@M00176:17:000000000-A0CNA:1:1:17738:1839 1:N:0:0 +TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCAGTTTTGTAAGTCTGACGTGAAATCCCCGGGCTCAACCTGGGAATTGCGTTGGAGACTGCAAGGCTGGAGTCTGGCAGAGGGGGGTAGAATTCCACGT ++ +BBCFDFFFHHHHHJJIHJIIJJIJJJJJIJJJJJJJG:FGHHJIBGHJHHHFFFDEEEEEDDEDEEEDDDDDDDDDDDDDDDBBDDDDDCDDDBDDCB@ACB@@@BBCCCDCA>>?+ +@M00176:17:000000000-A0CNA:1:1:12224:1840 1:N:0:0 +TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCGAAACTGGCAGGCTGTAGTCTTTGTGAGGGGGGTAGAATTCCAGGG ++ +@@@FFFFFFHHHHJJIIIJGIJJI>HIIIFIJIDIHGAHIJJIHHGHFDFDCB@@DBDDDDDDDA@?@;@B@ADDCDCCCDDBBBBBDCCBC@ABBCBCCC<:@@C@?25:CCDCB9918+44>(+((((+4:8@@@7998A>>+++(+(& +@M00176:17:000000000-A0CNA:1:1:14532:1840 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCGTTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCCAGAGGTAATCAGGGGGTACTGGAATTCCATGTG ++ +@@@DDDDDBDDHDIII@EHHGGIIIIGIIIIIIIIIIHHIIIG8=FHIGIIIEHE@8&5;889(4+:(+:?@?@CC3+83+:+4>@@:C3::8:>3+:(+(+:4:(+9(:(())&82&+2++8@CC(+>@CBBB&)(4>C(::>@(>>>4: +@M00176:17:000000000-A0CNA:1:1:13369:1840 1:N:0:0 +GAGCCGCTTAAAGCTACCAGTTATATTGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGA ++ +?@+ADDDDFFFFFII?GIEFHIHIII@A?B3::>A@ABB>A?B@BBBBBBBAB>@@???BB3:4:8?BBBBBBBBB9?8 +@M00176:17:000000000-A0CNA:1:1:17458:1840 1:N:0:0 +TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGACATTTAAGTCAGGTGTGAAATCCCAGAGCTCAACTCTGGAACTGCCTTTGATACTGGGTGTCTAGAGTATGGAAGAGGTGAGTGGAATTCCGAGT ++ +@@@DDDDDHFFDA+@?8?C?FFEHIIIHIIIIIGI9AEBHEFEAD8CBC<>AC@CCC<@CCC@@?:>CCC:>CC>C9CCCCCCACCE>@@3>?4:?BBB:(>?C@(4>@CCC9CDDCB?B9@>AACCC>BBBBCDD@B(4+4>>@CD?@BAC(:@A<>8:A3:AC? +@M00176:17:000000000-A0CNA:1:1:14003:1841 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCCAGAGGTAATCAGGGGGTACTGGAATTCAATGTG ++ +CCCFFFFFHHHHHIJIGHIIJJJJJJJJJJJJIIIFGGHJJHIIGIIJJJJJHHFBD0;@2B@>AADCDC<>ACDAC@44(+33><<<5>BD&(++8(4::@DCA>(+:94>@A(4@C@: +@M00176:17:000000000-A0CNA:1:1:16682:1842 1:N:0:0 +ACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTACATACCAAAGACGAGCGCCTTTACGC ++ +???D?DBDBDDDA2CEE;+>A>5>BA>AAAAAAADEABAADAA>>>AA3>19>>9>?;>>?>>?>>>>AA>A:>A>+:84>A31<>AAAABA<>A>939&00999>.9AAA?> +@M00176:17:000000000-A0CNA:1:1:14923:1843 1:N:0:0 +AAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAG ++ +@@@DFDFFHGGHFGIHIHIJIIHJJJJB?C*?DFEHEGFGHJECBFHI9?FHIJIJIJJHHHHFBDDCEEDDCC;CCDDDDDDD>@C@CDEDCCDDD5?CDDD>A@ABCCDDCD1>80.5.5@B<@<00)<(::??< +@M00176:17:000000000-A0CNA:1:1:12467:1843 1:N:0:0 +TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGGTTTTTTAAGTCTGTTGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGAAGACTTGGGTGCAGAGGAGGTAAGCGGAATATGTTGT ++ +???D=BDD=ADDDIIEIIIIIIIIII)C?D?DEII8?DDIII4A5@ADD@?A5;?805<=?=AAEEAA((:>?ADAAA??9AA??>+802(2&(98:::AA:3>>:>((((3>>A:2<;((4:2<<<;(:4:+5;?;?+(((+(3 +@M00176:17:000000000-A0CNA:1:1:16919:1843 1:N:0:0 +TCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGAATCAAGGCTCTTAAACCTG ++ +@?=DDDBDHF?DC9E2@CFD9ACEAHA??9?:DD9?BGGEB8;3=@>3@ACB@35<5>;;;;:3:>(4>3>C@@AC3)29B5>>ACCDCCB3@C3(+849&0&8(4>:?@?CCCA@:9?1 +@M00176:17:000000000-A0CNA:1:1:17484:1844 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCGCCTCCCTAGAGTTAATCAGGGGGGACTGGAATTCAATGTG ++ +CCCFFFFFHHHHFIJJJIJBHJIJJJIIJIIJJJJJBFHJEHIJGEHH@GIJHHFDD&5<8?334>C>AD@CDACD:>ACDCDCAAC:A>:CBCDCD@ACAAA(:4(3>:<<<5>2>&&0CCD +@M00176:17:000000000-A0CNA:1:1:18075:1844 1:N:0:0 +TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTAGGTGGTTTGTCGCGTCGTCTGTGAAATTCCGGGGCTTAACTCCGGGCGTGCATGCGATACGGGCATAACTTGAGTACTGAAGGGGTAACGGGAATTCCTGG ++ +B?@DDFFFDFHHHIIGHIIGIJIJIIGHHHGJIGGIDIJJJIIICEHD9ACB>@BDDDDDDD?BDDB>?BBDD<@ACDD@?5&5<94@CA@(:4>@D&5?4+0050>@? +@M00176:17:000000000-A0CNA:1:1:16762:1844 1:N:0:0 +TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTCTGA ++ +B@BDDFFFGHHHHA<ACBCCDEEFDBDDDDEED@C<ACCCCCB<9B@C:(:>@CC>CCA@CCC@CBBBCCCC0CCCBB?BBCCCC>:A?@A?CC>@AC>>C:9(&))55@AD:@4 +@M00176:17:000000000-A0CNA:1:1:14102:1846 1:N:0:0 +AACATAGGTCACGAGCATTATCCGGATTTATTGGGCGTAAAGGAAGCGTAGGCGGGGAGGTTGATCCATTGTTAAAAGCATTTGCTTAACAAATGTGTGCATTGGAGATCTCCTCCCTAGAGGTAATCAGGGGGGACTGGAATTCAATGGG ++ +CCCFFFFFHFHHHIJIHIJGIIIJIJJGJGIJIIJGIFHHGHCHIIIJEGIHHFF@B-7B8?3>ACD>AD:@@44>C@??C@CA@3A(:34@BCA:ABBD@:4(+(3+>C(:>3?>?9+3::(4>>C@CDCB@@&058>:((4:(( +@M00176:17:000000000-A0CNA:1:1:13058:1846 1:N:0:0 +CTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTAAGCACTCCGTGGACAGATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCT ++ +CCCFFFFFGHHHGJJEGIHIIIIIJIGIJJJJFGIFHIJ@GHHIJIIGHIJJIDFHJJIIIJHABCDBDDDDD<@CBBBDDDDD:>>@CDDDDDDDCCCCCDDDDDB@BC:?9@AAADDECCCDCCCCCDDDEECDEDB@9>BDD@CBB3& +@M00176:17:000000000-A0CNA:1:1:12794:1846 1:N:0:0 +TCAAAAGCAATATCAGCACCAACAGAAACAACCTGATTAGCGGCGTTGACAGATGTATCCATCTGAATGCAATGAAGAAAACCACCATTACCAGCATTAACCGTCAAACTATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTA ++ +B@BFFFFFHHHHGJJJJJJJIJJIIIIIIIIJIIIBGGIDFFGIHIIH9EEH>CDFFFEEEEEEDCCCCDD@CD@CDDCCDD@DDDACCCCCDDDCCCCDCABBDDDC@C>CCD@CDCC>>>@CDD +@M00176:17:000000000-A0CNA:1:1:15066:1846 1:N:0:0 +GAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTC ++ +@@@FDFFFHHHHGJJJJJJFHGGEHGIFHEGHIFGGIIJJIJJJBDHEHHJHIGBFEGFHIJCFGEGCCHHFDBBCDCCCC@CDDDDBA5<@@<>B>AC@C>CACC:@CA39<>A@<>8(>CC<4@:> +@M00176:17:000000000-A0CNA:1:1:15485:1847 1:N:0:0 +CGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGATTCAGGTTA ++ +BBBDFFFFHHHHFGEHHGIHIJJJJGGIIE?;FHHDHFDDFGIJJHIIFHJIFFHGGIJGIFFFBCDDDDDCD>ACDCDCCCDDDD@BBC@@CDDDDD??CCC>ACEDDDCB5>>9CCDCCAC@BBB<@@DDD(::(434>( diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/lane2_read1.fastq.gz Binary file test-data/split_libraries_fastq/lane2_read1.fastq.gz has changed diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/map.tsv --- a/test-data/split_libraries_fastq/map.tsv Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,35 +0,0 @@ -#SampleID BarcodeSequence LinkerPrimerSequence SampleType Year Month Day Subject ReportedAntibioticUsage DaysSinceExperimentStart Description -L1S8 AGCTGACTAGTC GTGCCAGCMGCCGCGGTAA gut 2008 10 28 1 Yes 0 1_Fece_10_28_2008 -L1S140 ATGGCAGCTCTA GTGCCAGCMGCCGCGGTAA gut 2008 10 28 2 Yes 0 2_Fece_10_28_2008 -L1S57 ACACACTATGGC GTGCCAGCMGCCGCGGTAA gut 2009 1 20 1 No 84 1_Fece_1_20_2009 -L1S208 CTGAGATACGCG GTGCCAGCMGCCGCGGTAA gut 2009 1 20 2 No 84 2_Fece_1_20_2009 -L1S76 ACTACGTGTGGT GTGCCAGCMGCCGCGGTAA gut 2009 2 17 1 No 112 1_Fece_2_17_2009 -L1S105 AGTGCGATGCGT GTGCCAGCMGCCGCGGTAA gut 2009 3 17 1 No 140 1_Fece_3_17_2009 -L1S257 CCGACTGAGATG GTGCCAGCMGCCGCGGTAA gut 2009 3 17 2 No 140 2_Fece_3_17_2009 -L1S281 CCTCTCGTGATC GTGCCAGCMGCCGCGGTAA gut 2009 4 14 2 No 168 2_Fece_4_14_2009 -L2S240 CATATCGCAGTT GTGCCAGCMGCCGCGGTAA left palm 2008 10 28 2 Yes 0 2_L_Palm_10_28_2008 -L2S155 ACGATGCGACCA GTGCCAGCMGCCGCGGTAA left palm 2009 1 20 1 No 84 1_L_Palm_1_20_2009 -L2S309 CGTGCATTATCA GTGCCAGCMGCCGCGGTAA left palm 2009 1 20 2 No 84 2_L_Palm_1_20_2009 -L2S175 AGCTATCCACGA GTGCCAGCMGCCGCGGTAA left palm 2009 2 17 1 No 112 1_L_Palm_2_17_2009 -L2S204 ATGCAGCTCAGT GTGCCAGCMGCCGCGGTAA left palm 2009 3 17 1 No 140 1_L_Palm_3_17_2009 -L2S357 CTAACGCAGTCA GTGCCAGCMGCCGCGGTAA left palm 2009 3 17 2 No 140 2_L_Palm_3_17_2009 -L2S222 CACGTGACATGT GTGCCAGCMGCCGCGGTAA left palm 2009 4 14 1 No 168 1_L_Palm_4_14_2009 -L2S382 CTCAATGACTCA GTGCCAGCMGCCGCGGTAA left palm 2009 4 14 2 No 168 2_L_Palm_4_14_2009 -L3S242 ACAGTTGCGCGA GTGCCAGCMGCCGCGGTAA right palm 2008 10 28 1 Yes 0 1_R_Palm_10_28_2008 -L3S378 ATCGATCTGTGG GTGCCAGCMGCCGCGGTAA right palm 2008 10 28 2 Yes 0 2_R_Palm_10_28_2008 -L3S294 CACGACAGGCTA GTGCCAGCMGCCGCGGTAA right palm 2009 1 20 1 No 84 1_R_Palm_1_20_2009 -L4S63 CTCGTGGAGTAG GTGCCAGCMGCCGCGGTAA right palm 2009 1 20 2 No 84 2_R_Palm_1_20_2009 -L3S313 AGTGTCACGGTG GTGCCAGCMGCCGCGGTAA right palm 2009 2 17 1 No 112 1_R_Palm_2_17_2009 -L3S341 CAAGTGAGAGAG GTGCCAGCMGCCGCGGTAA right palm 2009 3 17 1 No 140 1_R_Palm_3_17_2009 -L4S112 GCGTTACACACA GTGCCAGCMGCCGCGGTAA right palm 2009 3 17 2 No 140 2_R_Palm_3_17_2009 -L3S360 CATCGTATCAAC GTGCCAGCMGCCGCGGTAA right palm 2009 4 14 1 No 168 1_R_Palm_4_14_2009 -L4S137 GAACTGTATCTC GTGCCAGCMGCCGCGGTAA right palm 2009 4 14 2 No 168 2_R_Palm_4_14_2009 -L5S104 CAGTGTCAGGAC GTGCCAGCMGCCGCGGTAA tongue 2008 10 28 1 Yes 0 1_Tong_10_28_2008 -L5S240 CTGGACTCATAG GTGCCAGCMGCCGCGGTAA tongue 2008 10 28 2 Yes 0 2_Tong_10_28_2008 -L5S155 ATCTTAGACTGC GTGCCAGCMGCCGCGGTAA tongue 2009 1 20 1 No 84 1_Tong_1_20_2009 -L6S20 GAGGCTCATCAT GTGCCAGCMGCCGCGGTAA tongue 2009 1 20 2 No 84 2_Tong_1_20_2009 -L5S174 CAGACATTGCGT GTGCCAGCMGCCGCGGTAA tongue 2009 2 17 1 No 112 1_Tong_2_17_2009 -L5S203 CGATGCACCAGA GTGCCAGCMGCCGCGGTAA tongue 2009 3 17 1 No 140 1_Tong_3_17_2009 -L6S68 GATACGTCCTGA GTGCCAGCMGCCGCGGTAA tongue 2009 3 17 2 No 140 2_Tong_3_17_2009 -L5S222 CTAGAGACTCTT GTGCCAGCMGCCGCGGTAA tongue 2009 4 14 1 No 168 1_Tong_4_14_2009 -L6S93 GATTAGCACTCT GTGCCAGCMGCCGCGGTAA tongue 2009 4 14 2 No 168 2_Tong_4_14_2009 \ No newline at end of file diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/map.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_libraries_fastq/map.txt Sat Aug 05 07:27:17 2017 -0400 @@ -0,0 +1,1 @@ +#SampleID BarcodeSequence LinkerPrimerSequence Description s1 CTACCGATTGCG CCGGACTACHVGGGTWTCTAAT s1 s2 TCACCCAAGGTA CCGGACTACHVGGGTWTCTAAT s2 s3 AGCCAGTCATAC CCGGACTACHVGGGTWTCTAAT s3 s4 TAACGGCGCTCT CCGGACTACHVGGGTWTCTAAT s4 s5 GTTTGCTCGAGA CCGGACTACHVGGGTWTCTAAT s5 s6 CAAACGCACTAA CCGGACTACHVGGGTWTCTAAT s6 s7 GAACAAAGAGCG CCGGACTACHVGGGTWTCTAAT s7 s8 GCTAAGTGATGT CCGGACTACHVGGGTWTCTAAT s8 s9 AAGGGACAAGTG CCGGACTACHVGGGTWTCTAAT s9 s10 AGTGTCGATTCG CCGGACTACHVGGGTWTCTAAT s10 s11 CTATTAAGCGGC CCGGACTACHVGGGTWTCTAAT s11 s12 CCTACCATTGTT CCGGACTACHVGGGTWTCTAAT s12 s13 GAGTCCGTTGCT CCGGACTACHVGGGTWTCTAAT s13 s14 GATAACTGTACG CCGGACTACHVGGGTWTCTAAT s14 s15 TAAACCTGGACA CCGGACTACHVGGGTWTCTAAT s15 s16 CCGAATTGACAA CCGGACTACHVGGGTWTCTAAT s16 s17 CTGGCATCTAGC CCGGACTACHVGGGTWTCTAAT s17 s18 GGTGGTCGTTCT CCGGACTACHVGGGTWTCTAAT s18 s19 ACTATGGGCTAA CCGGACTACHVGGGTWTCTAAT s19 s20 GCATTGAGTTCG CCGGACTACHVGGGTWTCTAAT s20 s21 GTTGCTGAGTCC CCGGACTACHVGGGTWTCTAAT s21 s22 CTATGGTGAACC CCGGACTACHVGGGTWTCTAAT s22 s23 GGACCAAGGGAT CCGGACTACHVGGGTWTCTAAT s23 s24 GTATTGGTCAGA CCGGACTACHVGGGTWTCTAAT s24 s25 AGAACCGTCATA CCGGACTACHVGGGTWTCTAAT s25 s26 AACTGGAACCCT CCGGACTACHVGGGTWTCTAAT s26 s27 ATACTCGGCTGC CCGGACTACHVGGGTWTCTAAT s27 s28 ACGCTTAACGAC CCGGACTACHVGGGTWTCTAAT s28 s29 AGCTTACCGACC CCGGACTACHVGGGTWTCTAAT s29 s30 AGGGCTATAGTT CCGGACTACHVGGGTWTCTAAT s30 s31 TGTCTCGCAAGC CCGGACTACHVGGGTWTCTAAT s31 s32 CAGCCGCATATC CCGGACTACHVGGGTWTCTAAT s32 s33 GATACGTTCGCA CCGGACTACHVGGGTWTCTAAT s33 s34 CCAAGATTCGCC CCGGACTACHVGGGTWTCTAAT s34 s35 GAGGCTGATTTA CCGGACTACHVGGGTWTCTAAT s35 s36 GAGTTAGCATCA CCGGACTACHVGGGTWTCTAAT s36 s37 TGTAGTATAGGC CCGGACTACHVGGGTWTCTAAT s37 s38 CTCACGCAATGC CCGGACTACHVGGGTWTCTAAT s38 s39 GTCCCGTGAAAT CCGGACTACHVGGGTWTCTAAT s39 s40 GGACAGTGTATT CCGGACTACHVGGGTWTCTAAT s40 s41 ACACGACTATAG CCGGACTACHVGGGTWTCTAAT s41 s42 GTGTAGGTGCTT CCGGACTACHVGGGTWTCTAAT s42 s43 TGAACTAGCGTC CCGGACTACHVGGGTWTCTAAT s43 s44 TCCGAGTCACCA CCGGACTACHVGGGTWTCTAAT s44 s45 TCCTCTTTGGTC CCGGACTACHVGGGTWTCTAAT s45 s46 TCCACCCTCTAT CCGGACTACHVGGGTWTCTAAT s46 s47 TCGTGACGCTAA CCGGACTACHVGGGTWTCTAAT s47 s48 ACGGCTAGTTCC CCGGACTACHVGGGTWTCTAAT s48 s49 GCACTGGCATAT CCGGACTACHVGGGTWTCTAAT s49 s50 GGCATTAGTTGA CCGGACTACHVGGGTWTCTAAT s50 s51 CGGTAGTTGATC CCGGACTACHVGGGTWTCTAAT s51 s52 TGAAAGCGGCGA CCGGACTACHVGGGTWTCTAAT s52 s53 GGTTACGGTTAC CCGGACTACHVGGGTWTCTAAT s53 s54 ACATCAGGTCAC CCGGACTACHVGGGTWTCTAAT s54 s55 GTTGATACGATG CCGGACTACHVGGGTWTCTAAT s55 s56 CAGACACTTCCG CCGGACTACHVGGGTWTCTAAT s56 s57 TCACCATCCGAG CCGGACTACHVGGGTWTCTAAT s57 s58 ACCCACCACTAG CCGGACTACHVGGGTWTCTAAT s58 s59 CAGAAGGTGTGG CCGGACTACHVGGGTWTCTAAT s59 s60 GAAGCTTGAATC CCGGACTACHVGGGTWTCTAAT s60 s61 ACTAGGATCAGT CCGGACTACHVGGGTWTCTAAT s61 s62 GCTCCTTAGAAG CCGGACTACHVGGGTWTCTAAT s62 s63 TCCCATTCCCAT CCGGACTACHVGGGTWTCTAAT s63 s64 TGGCGTCATTCG CCGGACTACHVGGGTWTCTAAT s64 s65 AATCCTCGGAGT CCGGACTACHVGGGTWTCTAAT s65 s66 CTGGACGCATTA CCGGACTACHVGGGTWTCTAAT s66 s67 ACCGATTAGGTA CCGGACTACHVGGGTWTCTAAT s67 s68 ATGTGCTGCTCG CCGGACTACHVGGGTWTCTAAT s68 s69 TACGTACGAAAC CCGGACTACHVGGGTWTCTAAT s69 s70 ATCACATTCTCC CCGGACTACHVGGGTWTCTAAT s70 s71 AGCCTGGTACCT CCGGACTACHVGGGTWTCTAAT s71 s72 GCTAAAGTCGTA CCGGACTACHVGGGTWTCTAAT s72 s73 TCTCAGCGCGTA CCGGACTACHVGGGTWTCTAAT s73 s74 GACCCTAGACCT CCGGACTACHVGGGTWTCTAAT s74 s75 TATTCAGCGGAC CCGGACTACHVGGGTWTCTAAT s75 s76 GTTCCGGATTAG CCGGACTACHVGGGTWTCTAAT s76 s77 GCGTGTAATTAG CCGGACTACHVGGGTWTCTAAT s77 s78 CTGTAGCTTGGC CCGGACTACHVGGGTWTCTAAT s78 s79 ATGCCTCGTAAG CCGGACTACHVGGGTWTCTAAT s79 s80 ACCTATGGTGAA CCGGACTACHVGGGTWTCTAAT s80 s81 CTGTTACAGCGA CCGGACTACHVGGGTWTCTAAT s81 s82 CAGTCAGGCCTT CCGGACTACHVGGGTWTCTAAT s82 s83 ACTGAGCTGCAT CCGGACTACHVGGGTWTCTAAT s83 s84 ACGAAGTCTACC CCGGACTACHVGGGTWTCTAAT s84 s85 ACCGTCTTTCTC CCGGACTACHVGGGTWTCTAAT s85 s86 AGTCTGTCTGCG CCGGACTACHVGGGTWTCTAAT s86 s87 CCGCACTCAAGT CCGGACTACHVGGGTWTCTAAT s87 s88 TGTGGAAACTCC CCGGACTACHVGGGTWTCTAAT s88 s89 TTAGGCAGGTTC CCGGACTACHVGGGTWTCTAAT s89 s90 TAAGACTACTGG CCGGACTACHVGGGTWTCTAAT s90 s91 CGCGAAGTTTCA CCGGACTACHVGGGTWTCTAAT s91 s92 CGATACACTGCC CCGGACTACHVGGGTWTCTAAT s92 s93 TTGAAATCCCGG CCGGACTACHVGGGTWTCTAAT s93 s94 GTTAGGGAGCGA CCGGACTACHVGGGTWTCTAAT s94 s95 TTACTGTGGCCG CCGGACTACHVGGGTWTCTAAT s95 s96 ATATAAGGCCCA CCGGACTACHVGGGTWTCTAAT s96 s97 ACGTAACCACGT CCGGACTACHVGGGTWTCTAAT s97 s98 GTCGGAAATTGT CCGGACTACHVGGGTWTCTAAT s98 s99 TCTAACGAGTGC CCGGACTACHVGGGTWTCTAAT s99 s100 CATCTGGGCAAT CCGGACTACHVGGGTWTCTAAT s100 s101 TGTCCGTGGATC CCGGACTACHVGGGTWTCTAAT s101 s102 ACTCGGCCAACT CCGGACTACHVGGGTWTCTAAT s102 s103 GTTGGTTGGCAT CCGGACTACHVGGGTWTCTAAT s103 s104 TTCCACACGTGG CCGGACTACHVGGGTWTCTAAT s104 s105 AACCCAGATGAT CCGGACTACHVGGGTWTCTAAT s105 s106 GTAGTGTCAACA CCGGACTACHVGGGTWTCTAAT s106 s107 TGGAGAGGAGAT CCGGACTACHVGGGTWTCTAAT s107 s108 CGTATAAATGCG CCGGACTACHVGGGTWTCTAAT s108 s109 AATACAGACCTG CCGGACTACHVGGGTWTCTAAT s109 s110 GACTCAACCAGT CCGGACTACHVGGGTWTCTAAT s110 s111 GGAAGAAGTAGC CCGGACTACHVGGGTWTCTAAT s111 s112 ATCGATCCACAG CCGGACTACHVGGGTWTCTAAT s112 s113 ACACCGCACAAT CCGGACTACHVGGGTWTCTAAT s113 s114 GTCTCCTCCCTT CCGGACTACHVGGGTWTCTAAT s114 s115 GTAGCACTCATG CCGGACTACHVGGGTWTCTAAT s115 s116 CACCTGTAGTAG CCGGACTACHVGGGTWTCTAAT s116 s117 CACGAGCTACTC CCGGACTACHVGGGTWTCTAAT s117 s118 TCTCGATAAGCG CCGGACTACHVGGGTWTCTAAT s118 s119 TAGACACCGTGT CCGGACTACHVGGGTWTCTAAT s119 s120 AGACAAGCTTCC CCGGACTACHVGGGTWTCTAAT s120 s121 TCCGCAACCTGA CCGGACTACHVGGGTWTCTAAT s121 s122 TCACTTGGTGCG CCGGACTACHVGGGTWTCTAAT s122 s123 TTATGTACGGCG CCGGACTACHVGGGTWTCTAAT s123 s124 TTGGACGTCCAC CCGGACTACHVGGGTWTCTAAT s124 s125 TCCAGGGCTATA CCGGACTACHVGGGTWTCTAAT s125 s126 GCGTAGAGAGAC CCGGACTACHVGGGTWTCTAAT s126 s127 GAAACTCCTAGA CCGGACTACHVGGGTWTCTAAT s127 s128 ATCGGGCTTAAC CCGGACTACHVGGGTWTCTAAT s128 s129 TACGCCCATCAG CCGGACTACHVGGGTWTCTAAT s129 s130 AAGATCGTACTG CCGGACTACHVGGGTWTCTAAT s130 s131 ACTCATCTTCCA CCGGACTACHVGGGTWTCTAAT s131 s132 GAGATACAGTTC CCGGACTACHVGGGTWTCTAAT s132 s133 GCATGCATCCCA CCGGACTACHVGGGTWTCTAAT s133 s134 GATCTAATCGAG CCGGACTACHVGGGTWTCTAAT s134 s135 AATCTTGCGCCG CCGGACTACHVGGGTWTCTAAT s135 s136 GGAAATCCCATC CCGGACTACHVGGGTWTCTAAT s136 s137 GACCGTCAATAC CCGGACTACHVGGGTWTCTAAT s137 s138 TTGGAACGGCTT CCGGACTACHVGGGTWTCTAAT s138 s139 TCCTAGGTCCGA CCGGACTACHVGGGTWTCTAAT s139 s140 TCCTCACTATCA CCGGACTACHVGGGTWTCTAAT s140 s141 GCCTGCAGTACT CCGGACTACHVGGGTWTCTAAT s141 s142 GCCCAAGTTCAC CCGGACTACHVGGGTWTCTAAT s142 s143 ATAAAGAGGAGG CCGGACTACHVGGGTWTCTAAT s143 s144 GCGCCGAATCTT CCGGACTACHVGGGTWTCTAAT s144 s145 ATCCCAGCATGC CCGGACTACHVGGGTWTCTAAT s145 s146 GCTTCCAGACAA CCGGACTACHVGGGTWTCTAAT s146 s147 ACACAGTCCTGA CCGGACTACHVGGGTWTCTAAT s147 s148 ATTATACGGCGC CCGGACTACHVGGGTWTCTAAT s148 s149 ATTCAGATGGCA CCGGACTACHVGGGTWTCTAAT s149 s150 TAAACGCGACTC CCGGACTACHVGGGTWTCTAAT s150 s151 CCTCGGGTACTA CCGGACTACHVGGGTWTCTAAT s151 s152 TTCACCTGTATC CCGGACTACHVGGGTWTCTAAT s152 s153 CTCCAGGTCATG CCGGACTACHVGGGTWTCTAAT s153 s154 CAGGATTCGTAC CCGGACTACHVGGGTWTCTAAT s154 s155 CGCATACGACCT CCGGACTACHVGGGTWTCTAAT s155 s156 GCCTCGTACTGA CCGGACTACHVGGGTWTCTAAT s156 s157 ACCAACAGATTG CCGGACTACHVGGGTWTCTAAT s157 s158 GTGGCCTACTAC CCGGACTACHVGGGTWTCTAAT s158 s159 TTCCCTTCTCCG CCGGACTACHVGGGTWTCTAAT s159 s160 CATTTGACGACG CCGGACTACHVGGGTWTCTAAT s160 s161 AAGTGAAGCGAG CCGGACTACHVGGGTWTCTAAT s161 s162 TGCCGCCGTAAT CCGGACTACHVGGGTWTCTAAT s162 s163 AACCTCGGATAA CCGGACTACHVGGGTWTCTAAT s163 s164 GTGCTTGTGTAG CCGGACTACHVGGGTWTCTAAT s164 s165 CAACTAGACTCG CCGGACTACHVGGGTWTCTAAT s165 s166 AGTGCCCTTGGT CCGGACTACHVGGGTWTCTAAT s166 s167 GGAACGACGTGA CCGGACTACHVGGGTWTCTAAT s167 s168 TGTCAGCTGTCG CCGGACTACHVGGGTWTCTAAT s168 s169 CTGGTGCTGAAT CCGGACTACHVGGGTWTCTAAT s169 s170 GACAGAGGTGCA CCGGACTACHVGGGTWTCTAAT s170 s171 TCAGACCAACTG CCGGACTACHVGGGTWTCTAAT s171 s172 AGTGATGTGACT CCGGACTACHVGGGTWTCTAAT s172 s173 CTTAGCTACTCT CCGGACTACHVGGGTWTCTAAT s173 s174 TCGGTCCATAGC CCGGACTACHVGGGTWTCTAAT s174 s175 CACGTTTATTCC CCGGACTACHVGGGTWTCTAAT s175 s176 GAAACGGAAACG CCGGACTACHVGGGTWTCTAAT s176 s177 GGTCGTGTCTTG CCGGACTACHVGGGTWTCTAAT s177 s178 CGTCGTCTAAGA CCGGACTACHVGGGTWTCTAAT s178 s179 CAAGCGTTGTCC CCGGACTACHVGGGTWTCTAAT s179 s180 GACTTATGCCCG CCGGACTACHVGGGTWTCTAAT s180 s181 GTGACGTTAGTC CCGGACTACHVGGGTWTCTAAT s181 s182 GAGTCTTGGTAA CCGGACTACHVGGGTWTCTAAT s182 s183 TCGTCGCCAAAC CCGGACTACHVGGGTWTCTAAT s183 s184 AACATGCATGCC CCGGACTACHVGGGTWTCTAAT s184 s185 GTCTGTTGAGTG CCGGACTACHVGGGTWTCTAAT s185 s186 TGAGTTCGGTCC CCGGACTACHVGGGTWTCTAAT s186 s187 TTACGTGGCGAT CCGGACTACHVGGGTWTCTAAT s187 s188 CAATGCCTCACG CCGGACTACHVGGGTWTCTAAT s188 s189 TGTACGGATAAC CCGGACTACHVGGGTWTCTAAT s189 s190 AATCAACTAGGC CCGGACTACHVGGGTWTCTAAT s190 s191 GTGAGGGCAAGT CCGGACTACHVGGGTWTCTAAT s191 s192 CGTGGGCTCATT CCGGACTACHVGGGTWTCTAAT s192 \ No newline at end of file diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/seqs.fastq --- a/test-data/split_libraries_fastq/seqs.fastq Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,344 +0,0 @@ -@L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA -+ -ACCCGGGG@G?GGGGGGGGDGGGGGGGGGGGGGGGGDGGBGGGBGGGGGGGDGEGGDDG@GGGGEEEGGGGGG388GD>DDGGEE@DDDDB>FDGD>@-:74=;A*74====?BBD@ -@L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG -+ -GGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHFHBHHECCHBDHFEHAE@@BDB=B@@A2D(5553DAAAAE@EEA>;)9;==A>7B@=@78;:82?C<><9?<>>>98=@ -@L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA -+ -IIIGIIIIGIIIIIGIIHIDIHIHHF@IIFIIIIIGIIIHIHGHBIIIGHHGIFEEEFHHHDEEDG>DBEFBDB@BB=EEBBBEGEDDBA>D@@@@BCBB;BD@@>:@@>B@?=1@@@3<9767=6>;;? -@L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG -+ -IIIIIGIIIIIIIGIIIIIIIIIIIFIIGEGGDIIIIIFFHIIGDGEGD>GDGIIEIGIIFH>GBDDGFGGEGBB2DBDDGBDEBEBB8?B>A@D=38=AC>?>@@?DD@>=?=478;+;BE8>B -@L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG -+ -IIIIIHIIIIIIHEEIIEIIIIIIGGHIHEIIHDFCIGHHHCEDBIEIEEHBBEBBEIHGGGGGDHGGEEGDG?<2A5DBBBBEBEBBB@BEDEBBBE;@@@B@@8:@B@@@@AE@@A0@>=<9:;9=7>:;==DBD=@DBBB=D6'=;;A=?=99=9.9.2,/2===79@@6@ -@L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT -+ -IIIIIIIIGIIIDGIIIFIIHIIIIIIIIIIIIIIEIFGFGGGHIIGIIIHBHIHGIGIHI@EG@EGFFIFEDFBC=;;2?-?=A? -@L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG -+ -IIIIIIIIIIIIIIIIIIIIIIIIIBIIIGIIIIIIIIIIFIIIIIIIIIIIHGIIIIIIIEDGFGEDDGDBG:@@ECGEFEBHBHFHBB@@GGBBBDBECBBGECBECBFEBDEBEBB@@2B4?<=?;A??A@A+>? -@L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG -+ -IIIIIIIIIIIIHGGIIHIIIIIIIHIIIHIIIIIGIHHIIIIHDIIIHHHDIIIHIIHDHGEHEHGDGHEDGDHEEFEHG,EDCDCF@EBBECB@@B6>@A@ECBBCBBEEA>BBB@@DBAB=@8@@ -@L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT -+ -GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHGHHHHGBHHHHHHHHHFHHHHEHHHHEFEHFGGFGAA>C=GGDBDEDEEBCBBEEBB>BD@BB@DBBBB=C>BCCB>EBB8? -@L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG -+ -HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGBGGGEGGGG@GGDGHHFGHHHHHHGGFEGBGGG3CE>EAABGDBFFEDFE>E>DCB@EFFBC>EDBDD+CAA>>9?=99DCC?ACABEBBEBEECEBGDECDEEEEEED8B>@@@??EEB*B71;2>7@?===@ -@L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT -+ -IIIIIIIIHIIIHIIIIIIIIIIIIBIIIFIIIIIHIIHHHIIFFIIIFEIFIIIHIIIIIHGIEECHEFIDIBC>GBFFFEEHBEGEBBEFDBCBBF8A??ABBD=B@@DBB??A;;>? -@L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG -+ -IIIIIIIHIIIIIIIIIHIIIIIGIFIIIIIIIIIHIIIHHIHHFIIIGIIIEGIFHIIGHIIIGHGIGIGHIEDBFAEHHFEGGDGECBC@BDB@ -@L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG -+ -HHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHGHHHHHHHHHGFGGGFGGEGGGGGGHHHHHGHHHHHHHHHEEEGCHHHGGGHHHBHGH>EEDBDEHHHDFBFG>B -@L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG -+ -IIIIIIIIIIIIGIIIIIIIIIIHIEIIIHIIIGIIIIIIHIHFIIIIIDIHHIGGIIIIHBHIFIFIIIHHIEE@GBGDDDD@EHEEEECBEEEDCFBCBBFEEI>@B@DBDBEEB9???,?@A=A=<=;*;? -@L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG -+ -IIIIIIIIIIIIGIIIIIIDIIIIIHIIIHIIIIIHIIHHIIIIHIIIHHFHGIHHIGGIEHDICDEIEIIEIBBBD@FBEEBEEEFDDBEFEBBBBEBBBBD?BB88<:<9?B@B@@@A?3@@@9?@ -@L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA -+ -HHHHHHHHDAGGGHHHHHHHHHHHHHHHHHHHHHGHHHHHHH@HHHHGHHFGHHHHHHHFHHHHGHHHDHHGHGGHGBC>DECEDB>6>>>A6>>;4>=6:=9=B:@ -@L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG -+ -GGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEHHHHHHHFHHHEHEHHHHHHHBHHEHGHHHFGGGGBEEHEGCBGDCGEGGEGGEGECCEC@EE>B@A==@7,8=>;1=9A>>>;A -@L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG -+ -IIIIIIIIIIGIHHIIIIIGIIIIIFIIIHIIIIIHIIIHIIGIGIIIHIIFIIIGIIIDHGDIFIFEHIFBHBDFFFEIE8BDEDDEEFDDHECC@EABCEEBAD3D@BCCCEF>CBBBC8C?B@=@@<<>BB=@@B -@L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG -+ -GGGHHHHHGEGGGHHHHHHHHHHHHHHHHHGHHHHHBHHHHHHGCGBGEEGEEGGDBBADBGDFDEEGCGFEFFCDBBDBECCEFHEEEE:HB;A<::=?>=;EEEAE@>AC>,:.52?;:5255:,7@3:4:@ -@L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG -+ -IIIGIIIIIIIIGIIIIIIGIGIIHIIIFIHIIIIHHIIIIIEIEIHIDDGG@GGBGGGHIIIIGIFIGIIHHEEEGBHEHHFFGHGGGGEGDEBEDEACDDCABBDDB@@@BEBEBCCBDBBBBDEE?B@>B -@L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG -+ -GGGHHHHHBHHHHHHHHHHHHHHHHHHHHHGHHHHHGHHHHHBGHHHHHGHHBGGEGEDGBGGHHH>HDBGDGHHGHED>GB@GEGEGHFBHED@BDDDHFD@C>FCEFEHBB>@?:>??8826=8/:;<<:?>)>A -@L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG -+ -IIIIIIIIIIIIBHHIIHIHIIIIICIIIFIIIIIHIIIHGHHFEIHIEFIIBIHFHIIHIIGHEHGHGHHGHDBBICHEFHEGGDGEECGHEGDE>GGEDEGDDDBEEBEE@BBEBBDGDC=ABBBB?==-:==? -@L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG -+ -HIIIIIIIIIIIIIIIIIIIIIIIIIDIII3IIGGGGGIIIII@HIIGIIHGIIIIIHIIHIIIHIIDEGGEGGHHIHIHIIGDFIBFGFGGFGFEEGEFEF@EEEEFEBEDFDCD@BBEDE>EDCFBEDBEF>;A>:>AA8>>;AA -@L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG -+ -IIIIIIIIIIIIFIHIIIIGIIIIIFIIIHIIIIIIIIIIDIHIIIHIGIIHIIIIIIIIFHHIGHGIHIGEDBBBGBGHFHGCGHGEBEEIEEBEECEEHCHBEFBBB@BBBC>E@B?@@?@=@@BB;>@?>@?>A -@L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG -+ -DGGEBEEE?B,B?GGGGGGGGGGGGGGGGG>GGDGGGGGGGGGGGGGGGGGGGGGA>>G@>GDDGDGFFGD8FBB??>GDGDBA@AD;AAAE5?B;;4ADDBBB@B@D1.?8??-8::;? -@L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA -+ -IIIIIIIIIIIIHHHIIIIIIIIHIBIIIFIIIIIHIIIIIIEFIIFIEHFHHIIFIIIIHIHIEIHIHIIFICECIBHHIFEHDEGECBBHEDCBDFCEEBHBBEC>B=BBBCEBDA@BB>B>BB@CB=?@ -@L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG -+ -IIIIIIIIHIIIDIIIIIIIIIIIIFIIIHIIIIIHIIIIHIIIIIIIIIIIIIEIIIIIIIGIHHEIHIHHICEHIHHII@EHEHFEGDGHGEFEEFGGBEHBEEBBBEBEB@@>@=@B -@L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA -+ -IIIIHIHIEGGGEIIIIIIIIIIIIGIIIHIIIEGBGDDDDGGGIIIBEHIIFHHIIFIIGDBC@>EC3@?>@GEGG@@DBD@E8CC=BBDBEAC,38==:;=@?,3=9-<;68<===B -@L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT -+ -HHHHHHHHEGGGGHHHHHHHHHHHHFHHHHHHHDBGGFHHHHBGGDDDHHHHGGHHBFGFCGGFBEGGDGD>D>9ACACEEEBEEBBCDB@EF>DBB@C3*=@AA@=@BAEEBCCB@C -@L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG -+ -IIIIIIIIFIIIIIIIIIIIHIIIHHIIIHIIHIIHIIIFHIIFHIIIHFHIIDHHIHIIIIHIEHDIGIHGFDDCFCB@DEBFEFCFC>EDDBBBBBAAA?A@BB@E@?>-@A@B??@DB-A -@L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG -+ -IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIHIIGHIIIFHHIHIGIIIIHIIIIGHHIHIGHIECCGCFFIFFHDFGB=@@DBBBBBBDB?@EBBB=B>=B@BBBB -@L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG -+ -HIIIIIIIIIIIIDIIIHIIIIIIIBIIIGIIIIIGIGIHIFIHHIIIGHIIIFHHIGDFIEEIEFIDFHHGEGBHCFHGEGDGCDEBCCBBEEC>B@B>B8@BBB:B;???9@B>C@>;? -@L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC -+ -IIIIIIIIIHIIIHIIIIIIIIIIIIIIIIIIIIIIIHIHHHIIHIHIEIIFEHHIIIIIIIHIGIHIBDHEHGHHHGHHEGDBCDF>DDCBFE@?BB@B@78BB;@=@@?@=;A=????@:>=@=B -@L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG -+ -IIIIIIIIIIIIIIIIIIIIIIIIIGIIIHIIIIIHIIHHHIIIHIIIEEIFIIIFHIIIHHEIGGGIGIIGIGGDIEGGHGHDEEABCCEDBC@BBEBBDBFEEGABB@BCC<<<;:A0?A -@L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG -+ -IIIIGIIIIIIIIGGIIIIIIIIIIIIIIIIIIDIIIIIIIIHIHHHIIIHFIIHHIGIIIIHGEIDGFIIHIHFEIEHGIGHGGEG@EEBGEEBDEEEDGEHHDGHHGDDCGBDBB?DBD@B??A8A?@DBGGGGGGGDGGGGGGGG@GGGGGGGGGGGGGGGGGGGGGGGDGGDGCFF>?GGGGGGEGGGGGG8GGGGDGGBGCE>CECDEBEC?>,+?A=??==B@BBA<>>/=9?8?>>? -@L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG -+ -IIIIIIIIIIIIIFIIIIIGIIIIIBIIIFIIIGIFIIIHHHHIHIIIFHIGEIIHHIHHIH>IFIEIHHGHIBC>GCFEIEEHGEGDEEDGDBCBBBDDEEH@2@AEEB>BAA>>9>=AA77>>@? -@L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG -+ -BEEGGGGG3EBEDEEEEEGG3@>,EEGGADB??<16/459DFDGFFFDB15*9:/AAAAA(??;;FCCBDDD@>BBDBBB85>;A -@L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG -+ -BBBGGGGGDDGGGACA@,;<58;GDGGGGGGFGGBGGEGGGGGGGGEGEGEGBGEGGGEG8EEFEFEGFBFADCCEBDBFBEEEEAEBBBDD<>A8>>AEEBE@DBB8DB@B0>9)>>@=8@>:3:8@ -@L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG -+ -<@BGGGGGGGGGGGGGGGGGGGGGGDGGGGGEEGGGGGGGGGGGGGGGGGEGGGGGGBEGGGGGEGGGGGGGGBG2GBGDGGDEGDGFEGFGEGFFDEEGDEGEFDEGDDBDFC8FCBDDCCDDBFBC5AA>A>A8AAAA5@89? -@L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG -+ -IIIIIIIIGIIIHHIIIIIIIIIIIEIIIEIIIIIHIIHHHHHHHHIIGHEHIIIHIHFHICGHEFEIEIHEH@=BD@DEFECB:BBBCBCFCBBC=CCC@8E@CBBCBBBBBD8D@;AA@??BDDEGDBAAD=>@@@ -@L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG -+ -HHHHHHHHDGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHHFEHHHHEHHHHHHHHHHHDHHHHGGHGHGDHHHFGGGHHHHHHFHGHGHGCEE2EBA?CB@B@@BDBBB=B@=BBBBBBD7BBB -@L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG -+ -GGGGGGGGDGGGGHHHHHHFHHHHHHHHHHHHHHHHHHHHHHHHGHEHHHHHHHHEHFEFBGGHGGHHBEGEGFHBEHHBHGHHDHHHGHGBHC8>8?ECDEEEECB@/:::;5==>>A -@L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG -+ -IIIIIIIIIIIIIIIIIIIHIIIIHHIIIIIIIIIHIIIIFGHHHIGIGHHIIIIIIHIIHHIIGFFIHHHGICHGHGHGGHEDHHGDFGGGGGGDB@BCCBDABE@E@BBBBEEBF@?A>?==B@/=?@?>;@BB@ -@L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG -+ -IIIIIIIIIIIIIGHIIIIDIIIIIGIIIFIIIGIIIIIIIICGGIIFBDGG@IFFIFHGHIHDBECEHGHGFFD8FCGCGGGGFGEBDEEEBEEGEGBB>BB@BBCCA=;??=A@=?:>@??:@@(@ -@L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC -+ -IIIIIIIIIIIIIIIIIIIDIIIIIHGIIIIIIIIGIIIFIHIIHIIIGIHFIIHIIHIGHGGHEHEBEEEFCGEGDBEECEBBCC<@BDBBEDBDBD@BCBCDDDADB@==@?@B=@@B3B=@@ -@L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG -+ -HHHHHHHHHHDHFHHHHHHHHHHHHGHHHGHHHHHHHHHHHHHHHHHHGGGG>HHHHHEDGDDGFGGGDGGBGEEGBGG8G@DEGGEFEFEBBBDBBD@>?:;??=?B -@L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG -+ -HHHGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHDHHHHHHHHGG1D>D;A9A8C -@L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG -+ -HHHH@HHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHHHHHHHHHGEHHHFHEGEDGDDDGGFFFDDABEEEDDCFGEEDBCAEBEEEEE@EBBEBBEEEE8B@@:<8==<=BAA>A@:@8@ -@L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG -+ -IIIIIIIIIIIIIIHIIIIIIIIIIHIIIIIIIIIIIHIHFIIHIIHIHHHIIIHHIHHIIIHIGIHIFHIFHEDBIDFFEHHGGHHEFEBIEFFCEED>EDEBEEDBEEHDBEFEBDGEEDDBFEDEBCBBB;CBBB -@L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG -+ -IIIIIIIIIIIIIHIIIIIIIIIIIHIIIFIIIHIHIIHHIHHIIIIIHFIIGIFDAGIIHFHHEH@IHIIGIHGGIEHGHEHGHFHGEEEIHHEEE@EEEEIEFHBEDFGDFGGEEDEEBB18A@@@B@88@ -@L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG -+ -IIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIHIGIIGIIIIGIIIGIIIHIIIIIHHHIIIHIIIHIHHIIIHHGIGE>FEEEFHEEGGGGCHEHGGEGDEEGG@GDDEDGEBEGDBEEEDEBEBBDDCCDDC>DCC@C?CBCE@BBBB>9@<>?;@+;CCEB>B;/=?=BCBCB>BB>@@B<@?<85*928578=@B -@L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG -+ -GGGHGHHHHHHHGHHHHHHFHHHHHFHHHHHHHHHHHHHHHHHHEHHHEEGDGHHHHHHHHHHHGHHHHHHHHDGGHGHHHHFEHGGBHBEHEGHGHGGHGDHAEHEHBBDBBBBC>BEEEBBAEAAEEBEAB><@B -@L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG -+ -HHHHHHHHEHHHHHHHHHHGHHHHHHHHHDHHHHHHHHHHHHHHHHHHHHDHHHHEFHHHHH@HHHHHEHHEGEEEHDHEFHEGHE@EHGGHGC@B8DEEECEBEED8;?=??DBCDBCCCBDBBCECGGHH@GGCFGGEGHGB@BBBEEEEH@BBCCB@B>CE=A@@=:==? -@L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG -+ -EEEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGFGFGGGGDGFGGF>EGEGEFED8DGDEEEBGDGBDFDBDBDEE8>+>;;49;? -@L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT -+ -IIIIIHIDIIIIIDIIIIIIIIIIGDIIIEIIIIIIIIFFFIIIHIIIHEIIIIGFIIIGIIHIEHEIFDIFIGGGHEIEDHCGHGHHDEEIEEEEEEGGG>GDEDBB>ADAD@A;>4<=<967:<<=:? -@L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC -+ -HHHHHHHHHHHHHHDHHHHHHHHHHHGHHHHHHHHEHHGGBGGGHHHHHHHHHHGHHHHHHGEGFFGFFGFDFEFHFDHEEHBC2BEC8=??ABBB@D@@>BBBB@5@77==1CC@BB -@L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG -+ -GGGIIIIIIIIIIIHIIIIGIIIIIDIIIGIIIIIIIHIIIGIIGIIHEGGGCIIFIIIHIIIIHGHIHHIEHHEDDHFBIDFFHHFEGGGHG>EEBEBCEBC@BBBB=69=,:186:EBEBC;;@:@ -@L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA -+ -IIIIIIIIIIIIGIIIIHIIIIIIHHIIIIIIIIIHIIHHIIIIHIIIHGHHIIIHHIHIIIHIEEEIHIIGIFCDIBHHI@HHGEDD>B@DBEBBBEEEBEFCEGEEBE>>)>@EDCC@B<>:5:6:>68>0A;9>;:@?<>??@B -@L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG -+ -GGGHHHHHHHGHHHHHHHHGHHHHHGHHHHHHHHHHHHHHHHGHHHHHHHHHHHGBHHHHHBHHHHHHHFHGHBHHFGEGEEBEHHHDDDB@GB=?=?EEE>EBD3=@AABDDD>BBCAAECDCDB@BB@:C@BB@B=0=0>?=>@ -@L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG -+ -IIIIIIIHIIIIHIIIIIIIIIIIIIIIIIIIIHIIIIIIHIFIIIIIHHIFIIIHGHHIHIIIHHIIFFICIB8BDGD@DBACFEDGCEEBFFFCBBBEBEBAA;8?>?AAABBCC@BB>>C@DB@B@7@@@83;8@89<7+=8=(=5?9? -@L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG -+ -<@@GGGGGGGBGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGBGGGGGGDGGGB@>ADAEEE@D?A=<=@B@@@B@B>BBBBCA@@)>@<4>1>@@>;>A4:@A -@L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG -+ -IIIIIIIIIIIIIIIIIIIHIIIIIDIIIIIIIIIHIIHIIIIIHIIIHDIFIIIIIIIIIIHIGIGIHHIEIFFEIEIHIFIGGHGHDHHHGHGBB@@BBB=BBDB?@B:BBBEEECB??A4@4?==? -@L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG -+ -=B@GDGGGEGEGGGGGGGGEGGGGGDGGGGEEEGGGBGGGGGGGDGGGGGGG@EEBBE???DBGGGEGBGGDGGGGBC?0?BDGDDGCBDDDDDDDDEGGFDDDAG+(2:39DBAAA -@L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG -+ -IIIIIIIIHIIIIIIIIIIGIIIIFFIIIFIIIIIHIIIHEIIIHIIIHHIFIIIIHIIIHHHIGHHFHIHEIDBBFGHFHFEHEIIEBDBEEDCDEF=DBDDCBD=D@BDEBDFCC>B@B4B=@=>@=BB>8? diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/seqs.fna --- a/test-data/split_libraries_fastq/seqs.fna Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,172 +0,0 @@ ->L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA ->L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG ->L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA ->L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG ->L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ->L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ->L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT ->L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ->L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG ->L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT ->L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG ->L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTA ->L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT ->L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ->L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG ->L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ->L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG ->L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA ->L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG ->L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ->L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG ->L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ->L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ->L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ->L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG ->L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTA ->L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGC ->L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ->L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG ->L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ->L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ->L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA ->L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTT ->L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT ->L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG ->L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG ->L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG ->L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC ->L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG ->L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG ->L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGG ->L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG ->L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGC ->L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG ->L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG ->L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ->L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGA ->L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCT ->L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ->L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG ->L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ->L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG ->L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ->L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ->L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC ->L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG ->L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG ->L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ->L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG ->L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG ->L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG ->L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGC ->L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ->L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ->L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGT ->L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGG ->L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGG ->L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ->L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ->L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ->L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG ->L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT ->L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC ->L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG ->L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ->L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG ->L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA ->L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG ->L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGG ->L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG ->L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG ->L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG ->L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ->L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCG ->L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG ->L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/seqs.qual --- a/test-data/split_libraries_fastq/seqs.qual Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,584 +0,0 @@ ->L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[32 34 34 34 38 38 38 38 31 38 30 38 38 38 38 38 38 38 38 35 38 38 38 38 38 - 38 38 38 38 38 38 38 38 38 38 38 35 38 38 33 38 38 38 33 38 38 38 38 38 38 - 38 35 38 36 38 38 35 35 38 31 38 38 38 38 36 36 36 38 38 38 38 38 38 18 23 - 23 38 35 29 35 35 38 38 36 36 31 35 35 35 35 33 29 37 35 38 35 29 31 12 25 - 22 19 28 26 32 9 22 19 28 28 28 28 30 33 33 35 31] ->L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 37 - 39 33 39 39 36 34 34 39 33 35 39 37 36 39 32 36 31 31 33 35 33 28 33 31 31 - 32 17 35 7 20 20 20 18 35 32 32 32 32 36 31 36 36 32 29 26 8 24 26 28 28 - 32 29 22 33 31 28 31 22 23 26 25 23 17 30 34 27 29 27 24 30 27 29 29 29 24 - 23 28 31] ->L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 38 40 40 40 40 38 40 40 40 40 40 38 40 40 39 40 35 40 39 40 39 39 - 37 31 40 40 37 40 40 40 40 40 38 40 40 40 39 40 39 38 39 33 40 40 40 38 39 - 39 38 40 37 36 36 36 37 39 39 39 35 36 36 35 38 29 35 33 36 37 33 35 33 31 - 33 33 28 36 36 33 33 33 36 38 36 35 35 33 32 29 35 31 31 31 31 33 34 33 33 - 26 33 35 31 31 29 25 31 31 29 33 31 30 28 16 31 31 31 18 27 24 22 21 22 28 - 21 29 26 26 30] ->L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 38 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 40 - 37 40 40 38 36 38 38 35 40 40 40 40 40 37 37 39 40 40 38 35 38 36 38 35 29 - 38 35 38 40 40 36 40 38 40 40 37 39 29 38 33 35 35 38 37 38 38 36 38 33 33 - 17 35 33 35 35 38 33 35 36 33 36 33 33 23 30 27 33 29 33 29 32 31 35 28 18 - 23 28 32 34 29 30 29 31 31 30 35 35 31 29 28 30 28 19 22 23 26 10 26 33 36 - 23 29 33] ->L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 39 40 40 40 40 40 40 39 36 36 40 40 36 40 40 40 40 40 40 38 - 38 39 40 39 36 40 40 39 35 37 34 40 38 39 39 39 34 36 35 33 40 36 40 36 36 - 39 33 33 36 33 33 36 40 39 38 38 38 38 38 35 39 38 38 36 36 38 35 38 30 27 - 17 32 20 35 33 33 33 33 36 33 36 33 33 33 31 33 36 35 36 33 33 33 36 26 31 - 31 31 33 31 31 23 25 31 33 31 31 31 31 32 36 31 31 32 15 31 29 28 27 24 25 - 26 24 28 22 29 25 26 28 28 27 30] ->L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[39 39 39 38 38 36 38 36 38 38 38 38 38 39 39 39 39 39 39 39 38 39 39 38 38 - 34 38 38 39 37 39 39 39 39 39 39 39 39 36 35 33 38 38 39 37 39 39 39 39 37 - 39 39 39 39 39 37 39 39 39 39 39 36 33 37 34 36 36 35 37 37 37 35 36 28 26 - 31 34 28 34 34 34 32 32 33 33 35 31 31 25 28 26 30 28 33 33 31 29 35 33 35 - 28 31 35 33 33 33 28 35 21 6 28 26 26 32 28 30 28 24 24 28 24 13 24 13 17 - 11 14 17 28 28 28 22 24 31 31 21 31] ->L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 40 40 40 38 40 40 40 35 38 40 40 40 37 40 40 39 40 40 40 40 - 40 40 40 40 40 40 40 40 40 40 36 40 37 38 37 38 38 38 39 40 40 38 40 40 40 - 39 33 39 40 39 38 40 38 40 39 40 31 36 38 31 36 38 37 37 40 37 36 35 37 33 - 34 27 37 36 34 31 33 10 31 33 33 33 33 23 17 28 28 30 33 33 31 27 33 33 31 - 33 33 8 32 32 31 23 28 31 30 31 31 20 30 27 29 28 26 26 17 30 12 30 28 32 - 30] ->L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 33 40 40 40 38 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 - 40 40 39 38 40 40 40 40 40 40 40 36 35 38 37 38 36 35 35 38 35 33 38 25 31 - 31 36 34 38 36 37 36 33 39 33 39 37 39 33 33 31 31 38 38 33 33 33 35 33 36 - 34 33 33 38 36 34 33 36 34 33 37 36 33 35 36 33 36 33 33 31 31 17 33 19 30 - 27 28 30 26 32 30 30 32 31 32 10 29 30] ->L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 39 38 38 40 40 39 40 40 40 40 40 40 40 - 39 40 40 40 39 40 40 40 40 40 38 40 39 39 40 40 40 40 39 35 40 40 40 39 39 - 39 35 40 40 40 39 40 40 39 35 39 38 36 39 36 39 38 35 38 39 36 35 38 35 39 - 36 36 37 36 39 38 11 36 35 34 35 34 37 31 36 33 33 36 34 33 31 31 33 21 29 - 31 32 31 36 34 33 33 34 33 33 36 36 32 29 33 33 33 31 31 35 33 32 33 28 31 - 23 31 31] ->L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 38 39 39 39 39 38 39 39 39 39 38 33 39 39 39 39 39 - 39 39 39 39 37 39 39 39 39 36 39 39 39 39 36 37 36 39 37 38 38 37 38 32 32 - 29 34 28 38 38 35 33 35 36 35 36 36 33 34 33 33 36 36 33 33 29 33 35 31 33 - 33 31 35 33 33 33 33 28 34 29 33 34 34 33 29 36 33 33 23 30] ->L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 38 33 38 38 38 36 38 38 38 38 31 38 38 35 38 39 39 - 37 38 39 39 39 39 39 39 38 38 37 36 38 33 38 38 38 18 34 36 29 36 32 32 33 - 38 35 33 37 37 36 35 37 36 29 36 29 35 34 33 31 36 37 37 33 34 29 36 35 33 - 35 35 10 34 32 32 29 29 24 30 28 24 24 35 34 34 30 32 34 32 33 36 33 33 36 - 33 27 33 33 35 31 33 31 31 31 33] ->L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 - 40 40 40 40 40 40 40 40 40 40 39 40 40 38 40 40 38 40 40 40 40 39 40 40 40 - 40 35 38 40 33 37 39 35 39 33 36 39 39 40 36 35 39 38 39 39 40 37 37 38 38 - 39 31 29 36 36 34 36 33 38 35 36 34 35 36 36 36 36 36 36 35 23 33 29 31 31 - 31 30 30 36 36 33 9 33 22 16 26 17 29 22 31 30 28 28 28 31] ->L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 - 33 40 40 40 37 40 40 40 40 40 39 40 40 39 39 39 40 40 37 37 40 40 40 37 36 - 40 37 40 40 40 39 40 40 40 40 40 39 38 40 36 36 34 39 36 37 40 35 40 33 34 - 29 38 33 37 37 37 36 36 39 33 36 38 36 33 33 36 37 35 33 34 33 33 37 23 32 - 30 30 32 33 33 35 28 33 31 31 35 33 33 30 30 32 26 26 29 30] ->L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 38 40 - 37 40 40 40 40 40 40 40 40 40 39 40 40 40 39 39 40 39 39 37 40 40 40 38 40 - 40 40 36 38 40 37 39 40 40 38 39 40 40 40 38 39 38 40 38 40 38 39 40 36 35 - 33 37 32 36 39 39 37 36 38 38 35 38 36 27 34 33 37 34 38 35 36 38 38 38 38 - 36 38 39 36 36 34 36 29 34 33 34 31 33 35 33 31] ->L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 35 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 37 38 38 38 37 38 - 38 36 38 38 38 38 38 38 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 36 36 - 36 38 34 39 39 39 38 38 38 39 39 39 33 39 38 39 29 36 36 35 33 35 36 39 39 - 39 35 37 33 37 38 29 27 30 30 34 32 34 23 35 35 32 33 35 36 36 33 36 35 33 - 31 33 36 33 31 33 29 33] ->L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 40 - 36 40 40 40 39 40 40 40 38 40 40 40 40 40 40 39 40 39 37 40 40 40 40 40 35 - 40 39 39 40 38 38 40 40 40 40 39 33 39 40 37 40 37 40 40 40 39 39 40 36 36 - 31 38 33 38 35 35 35 35 31 36 39 36 36 36 36 34 33 36 36 36 35 34 37 33 34 - 33 33 37 36 36 40 29 31 33 31 35 33 35 33 36 36 33 24 30 30 30 11 30 27 32 - 29 31 32 28 32 28 27 28 26 9 26 30] ->L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 35 40 40 40 40 40 - 39 40 40 40 39 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 39 - 37 39 38 40 39 39 40 38 38 40 36 39 35 40 34 35 36 40 36 40 40 36 40 33 33 - 33 35 31 37 33 36 36 33 36 36 36 37 35 35 33 36 37 36 33 33 33 33 36 33 33 - 33 33 35 30 33 33 23 23 27 25 27 24 30 33 31 33 31 31 31 32 30 18 31 31 31 - 24 30 31] ->L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[39 39 39 39 39 39 39 39 35 32 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 31 39 39 39 39 38 39 39 - 37 38 39 39 39 39 39 39 39 37 39 39 39 39 38 39 39 39 35 39 39 38 39 38 38 - 39 27 38 36 31 35 36 33 36 37 36 37 39 38 38 38 33 38 31 33 33 36 35 33 35 - 33 31 35 36 33 37 36 36 38 36 36 29 38 33 34 29 35 36 34 36 35 33 29 21 29 - 29 29 32 21 29 29 26 19 29 28 21 25 28 24 28 33 25 31] ->L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 36 39 39 39 39 39 39 39 37 39 39 39 36 39 36 39 39 39 39 39 39 39 33 - 39 39 36 39 38 39 39 39 37 38 38 38 38 33 36 36 39 36 38 34 33 38 35 34 38 - 36 38 38 36 38 38 36 38 36 34 34 36 34 31 36 36 29 33 31 32 28 28 31 22 11 - 23 28 29 26 16 28 24 32 29 29 29 26 32] ->L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 38 40 39 39 40 40 40 40 40 38 40 40 40 40 40 - 37 40 40 40 39 40 40 40 40 40 39 40 40 40 39 40 40 38 40 38 40 40 40 39 40 - 40 37 40 40 40 38 40 40 40 35 39 38 35 40 37 40 37 36 39 40 37 33 39 33 35 - 37 37 37 36 40 36 23 33 35 36 35 35 36 36 37 35 35 39 36 34 34 31 36 32 33 - 34 36 36 33 32 35 18 35 31 33 34 34 34 36 37 29 34 33 33 33 34 23 34 30 33 - 31 28 31 31 27 27 29 33 33 28 31 31 33] ->L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[38 38 38 39 39 39 39 39 38 36 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 38 39 39 39 39 39 33 39 39 39 39 39 39 38 34 38 33 38 36 36 - 38 36 36 38 38 35 33 33 32 35 33 38 35 37 35 36 36 38 34 38 37 36 37 37 34 - 35 33 33 35 33 36 34 34 36 37 39 36 36 36 36 25 39 33 26 32 27 25 25 28 30 - 29 28 26 36 36 36 32 36 31 29 32 34 29 11 25 13 20 17 30 26 25 20 17 20 20 - 25 11 22 31 18 25 19 25 31] ->L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 38 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 38 40 38 40 40 39 - 40 40 40 37 40 39 40 40 40 40 39 39 40 40 40 40 40 36 40 36 40 39 40 35 35 - 38 38 31 38 38 33 38 38 38 39 40 40 40 40 38 40 37 40 38 40 40 39 39 36 36 - 36 38 33 39 36 39 39 37 37 38 39 38 38 38 38 36 38 35 36 33 36 35 36 32 34 - 35 35 34 32 33 33 35 35 33 31 31 31 33 36 33 36 33 34 34 33 35 33 33 33 33 - 35 36 36 30 33 31 29 33] ->L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[38 38 38 39 39 39 39 39 33 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 38 39 39 39 39 39 38 39 39 39 39 39 33 38 39 39 39 39 39 38 - 39 39 33 38 38 36 38 36 35 38 33 38 38 39 39 39 29 39 35 33 38 35 38 39 39 - 38 39 36 35 29 38 33 31 38 36 38 36 38 39 37 33 39 36 35 31 33 35 35 35 39 - 37 35 31 34 29 37 34 36 37 36 39 33 33 29 31 30 25 29 30 30 23 23 17 21 28 - 23 14 25 26 27 27 25 30 29 8 29 32] ->L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 33 39 39 40 40 39 40 39 40 40 40 40 40 - 34 40 40 40 37 40 40 40 40 40 39 40 40 40 39 38 39 39 37 36 40 39 40 36 37 - 40 40 33 40 39 37 39 40 40 39 40 40 38 39 36 39 38 39 38 39 39 38 39 35 33 - 33 40 34 39 36 37 39 36 38 38 35 38 36 36 34 38 39 36 38 35 36 29 38 38 36 - 35 36 38 35 35 35 33 36 36 33 36 36 31 33 33 36 33 33 35 38 35 34 28 32 33 - 33 33 33 30 28 28 12 25 28 28 30] ->L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 40 35 40 40 40 18 40 40 38 38 38 38 38 40 40 40 40 40 31 39 40 40 38 40 40 - 39 38 40 40 40 40 40 39 40 40 39 40 40 40 39 40 40 35 36 38 38 36 38 38 39 - 39 40 39 40 39 40 40 38 35 37 40 33 37 27 35 33 35 35 33 40 35 39 39 40 40 - 38 38 38 37 40 37 36 39 32 32 34 36 32 36 40 35 40 24 40 40 37 34 37 37 36 - 36 36 37 27 33 33 27 35 28 28 30 30 30 27 31] ->L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 40 40 40 - 38 40 40 40 39 40 40 40 40 40 38 40 39 39 39 39 39 39 40 39 40 39 40 35 36 - 40 38 34 40 37 39 39 40 39 36 40 39 36 39 36 39 36 40 36 40 39 36 40 33 35 - 33 37 33 37 34 35 35 35 38 36 38 36 36 35 35 35 38 34 33 35 33 33 35 32 33 - 36 33 36 35 38 35 36 32 36 33 34 33 34 33 33 36 33 33 31 36 33 22 33 33 31 - 33 33 33 21 30] ->L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[34 34 34 38 33 38 38 38 36 38 38 38 38 38 38 38 36 38 38 38 38 38 38 38 38 - 35 38 38 38 38 38 36 35 38 38 37 38 38 38 38 38 38 38 38 37 35 35 38 37 38 - 38 36 38 38 38 33 36 38 36 38 35 38 38 38 37 38 29 38 37 38 38 37 38 37 36 - 36 38 36 37 36 37 31 36 36 36 36 37 36 33 36 35 37 35 34 35 31 33 33 36 35 - 36 29 36 35 34 37 33 36 35 33 36 37 29 26 32 29 25 29 32 32 23 29 29 26 32 - 32] ->L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 37 40 39 40 40 40 40 38 40 40 40 40 40 - 37 40 40 40 39 40 40 40 40 40 40 40 40 40 40 35 40 39 40 40 40 39 40 38 40 - 40 39 40 40 40 40 40 40 40 40 37 39 39 40 38 39 38 40 39 40 38 36 35 33 33 - 33 38 33 38 39 37 39 38 34 38 39 38 36 33 36 36 40 36 36 33 36 36 34 36 36 - 39 34 39 33 36 37 33 33 33 31 33 33 33 34 29 36 31 33 30 31 31 30 31 28 31 - 31 33 33 26 29 31 30 29 31 30 29 32] ->L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[35 38 38 36 33 36 36 36 30 33 11 33 30 38 38 38 38 38 38 38 38 38 38 38 38 - 38 38 38 38 38 29 38 38 35 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 38 38 38 38 38 32 29 29 38 31 29 38 27 36 30 33 36 36 31 38 38 35 38 38 35 - 38 33 35 38 36 33 29 35 35 38 35 38 37 37 38 35 23 37 33 33 30 30 29 38 35 - 38 35 33 32 31 32 35 26 32 32 32 36 20 30 33 26 26 19 32 35 35 33 33 33 31 - 33 31 35 16 13 30 23 30 30 12 23 25 25 26 30] ->L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 40 40 39 40 - 33 40 40 40 37 40 40 40 40 40 39 40 40 40 40 40 40 36 37 40 40 37 40 36 39 - 37 39 39 40 40 37 40 40 40 40 39 40 39 40 36 40 39 40 39 40 40 37 40 34 36 - 34 40 33 39 39 40 37 36 39 35 36 38 36 34 33 33 39 36 35 34 33 35 37 34 36 - 36 33 39 33 33 36 34 29 33 28 33 33 33 34 36 33 35 32 31 33 33 29 33 29 33 - 33 31 34 33 28 30 31] ->L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[40 40 40 40 40 40 40 40 39 40 40 40 35 40 40 40 40 40 40 40 40 40 40 40 40 - 37 40 40 40 39 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 - 40 40 40 40 36 40 40 40 40 40 40 40 38 40 39 39 36 40 39 40 39 39 40 34 36 - 39 40 39 39 40 40 31 36 39 36 39 37 36 38 35 38 39 38 36 37 36 36 37 38 38 - 33 36 39 33 36 36 27 37 31 31 33 35 35 35 34 33 35 33 35 35 33 33 33 29 33 - 33 33 36 33 36 33 31 31 29 31 28 31 33] ->L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 39 40 39 40 36 38 38 38 36 40 40 40 40 40 40 40 40 40 40 40 40 - 38 40 40 40 39 40 40 40 36 38 33 38 35 35 35 35 38 38 38 40 40 40 33 36 39 - 40 40 37 39 39 40 40 37 40 40 38 35 33 34 31 29 36 34 18 31 30 29 31 38 36 - 38 38 31 31 35 33 35 31 36 23 34 34 28 33 33 35 33 36 32 34 11 18 23 28 28 - 25 26 28 31 30 11 18 28 24 12 27 26 27 30 32 30 30] ->L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 - 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 36 39 39 39 39 39 - 39 39 39 39 39 38 39 39 39 39 39 39 38 36 33 36 36 38 36 38 38 37 38 34 34 - 33 38 31 36 36 37 35 36 36 36 38 35 36 36 33 33 39 36 35 36 33 36 36 38 38 - 33 36 38 33 32 36 33 35 31 32 39 35 34 30 34 36 31 30 33 35 33 25 29 21 23 - 27 28 28 28 33] ->L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[39 39 39 39 39 39 39 39 36 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 37 39 39 39 39 39 39 39 35 33 38 38 37 39 39 39 39 33 38 38 35 35 35 39 39 - 39 39 38 38 39 39 33 37 38 37 34 38 38 37 33 36 38 38 35 38 35 29 35 29 24 - 32 34 32 34 36 36 36 33 36 36 33 33 34 35 33 31 36 37 29 35 33 33 31 34 18 - 9 28 31 32 32 31 28 31 33 32 36 36 33 34 34 33 31 34] ->L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 39 - 39 40 40 40 39 40 40 39 40 40 39 40 40 40 37 39 40 40 37 39 40 40 40 39 37 - 39 40 40 35 39 39 40 39 40 40 40 40 39 40 36 39 35 40 38 40 39 38 37 35 35 - 34 37 34 33 31 35 36 33 37 36 37 34 37 34 29 36 35 35 33 33 33 33 33 32 32 - 32 30 32 31 33 33 31 36 31 30 29 12 31 32 31 33 30 30 31 35 33 12 32] ->L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 38 39 40 40 40 37 39 - 39 40 39 40 38 40 40 40 40 39 40 40 40 40 38 39 39 40 39 40 38 39 40 36 34 - 34 38 34 37 37 40 37 37 39 35 37 38 33 28 31 31 35 33 33 33 33 33 33 35 33 - 30 31 36 33 33 33 28 33 29 28 33 31 33 33 33 33] ->L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[39 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 39 40 40 40 40 40 40 40 - 33 40 40 40 38 40 40 40 40 40 38 40 38 40 39 40 37 40 39 39 40 40 40 38 39 - 40 40 40 37 39 39 40 38 35 37 40 36 36 40 36 37 40 35 37 39 39 38 36 38 33 - 39 34 37 39 38 36 38 35 38 34 35 36 33 34 34 33 33 36 36 34 29 33 31 33 29 - 33 23 31 33 33 33 25 33 26 30 30 30 24 31 33 29 34 31 29 26 30] ->L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 - 40 40 40 40 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 39 40 39 40 36 40 - 40 37 36 39 39 40 40 40 40 40 40 40 39 40 38 40 39 40 33 35 39 36 39 38 39 - 39 39 38 39 39 36 38 35 33 34 35 37 29 35 35 34 33 37 36 31 30 33 33 31 33 - 31 22 23 33 33 26 31 28 31 31 30 31 28 26 32 28 30 30 30 30 31 25 29 28 31 - 28 33] ->L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 38 40 40 40 39 40 40 40 40 40 39 40 40 39 39 39 40 40 40 39 40 40 40 36 36 - 40 37 40 40 40 37 39 40 40 40 39 39 36 40 38 38 38 40 38 40 40 38 40 38 38 - 35 40 36 38 38 39 38 39 35 36 36 32 33 34 34 36 35 33 34 31 33 33 36 33 33 - 35 33 37 36 36 38 32 33 33 31 33 34 34 27 27 30 6 10 28 29 27 27 26 25 32 - 15 30 32] ->L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 38 40 40 40 40 40 40 40 40 38 38 40 40 40 40 40 40 40 40 40 40 - 40 40 40 40 40 40 40 40 35 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 40 - 39 37 40 40 39 39 40 38 40 40 40 40 39 38 36 40 35 38 37 40 40 39 40 39 37 - 36 40 36 39 38 40 38 39 38 38 36 38 31 36 36 33 38 36 36 33 35 36 36 36 35 - 38 36 39 39 35 38 39 39 38 35 35 34 38 33 35 33 33 30 35 33 35 31 33 30 30 - 32 23 32 30 31 35 27 33 22 12 30] ->L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[32 29 33 38 38 38 38 38 38 38 35 38 38 38 38 38 38 38 38 31 38 38 38 38 38 - 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 35 38 38 35 38 34 37 - 37 29 30 38 38 38 38 38 38 36 38 38 38 38 38 38 23 38 38 38 38 35 38 38 33 - 38 27 38 36 38 27 38 38 36 35 36 38 38 38 38 36 36 38 16 28 30 30 33 36 37 - 35 29 34 36 29 34 36 34 35 36 33 36 34 30 29 11 10 30 32 28 30 30 28 28 33 - 31 33 33 32 27 29 29 14 28 24 30 23 30 29 29 30] ->L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 38 40 40 40 40 40 - 33 40 40 40 37 40 40 40 38 40 37 40 40 40 39 39 39 39 40 39 40 40 40 37 39 - 40 38 36 40 40 39 39 40 39 39 40 39 29 40 37 40 36 40 39 39 38 39 40 33 34 - 29 38 34 37 36 40 36 36 39 38 36 38 35 36 36 35 38 35 33 34 33 33 33 35 35 - 36 36 39 31 27 33 33 33 36 33 34 33 36 36 37 36 36 33 36 36 36 31 32 25 30 - 31 33 33 27 20 23 6 17 27 27 27 21 24 26 30 32] ->L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[36 37 37 38 38 38 38 38 38 33 35 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 38 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 33 38 - 36 38 38 36 38 31 38 38 38 38 36 37 37 38 36 34 38 38 34 34 36 32 34 35 35 - 35 23 38 26 31 32 31 31 31 33 35 35 33 37 36 39 36 35 33 29 17 31 32 36 36 - 33 29 33 32 32 29 29 24 29 28 32 32 22 22 29 29 31 30] ->L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[33 36 36 38 38 38 38 38 18 36 33 36 35 36 36 36 36 36 38 38 18 27 38 36 36 - 35 27 36 38 38 31 36 35 38 38 33 27 38 36 36 36 36 32 33 13 19 33 33 20 29 - 31 29 11 36 36 27 36 36 38 38 38 38 35 38 37 38 38 38 29 38 38 32 35 33 30 - 30 27 16 21 14 19 20 24 35 37 35 38 37 37 37 35 33 16 20 9 24 25 14 32 32 - 32 32 32 7 30 30 26 26 37 34 34 33 35 35 35 31 29 33 33 35 33 33 33 23 20 - 29 26 32] ->L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[33 33 33 38 38 38 38 38 35 35 38 38 38 32 34 32 31 11 26 27 20 23 26 38 35 - 38 38 38 38 38 38 37 38 38 33 38 38 36 38 38 38 38 38 38 38 38 36 38 36 38 - 36 38 33 38 36 38 38 38 36 38 23 36 36 37 36 37 36 38 37 33 37 32 35 34 34 - 36 33 35 33 37 33 36 36 36 36 32 36 33 33 33 35 35 27 29 32 23 29 29 32 36 - 36 33 36 31 35 33 33 23 35 33 31 33 15 29 24 8 29 29 31 28 23 31 29 25 18 - 25 23 31] ->L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[27 31 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 35 38 38 38 38 38 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 36 38 38 38 38 38 38 33 36 38 38 38 38 38 36 38 38 38 38 38 38 38 38 33 38 - 17 38 33 38 35 38 38 35 36 38 35 38 37 36 38 37 38 36 38 37 37 35 36 36 38 - 35 36 38 36 37 35 36 38 35 35 33 35 37 34 23 37 34 33 35 35 34 34 35 35 33 - 37 33 34 20 32 32 29 32 29 32 27 32 32] ->L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 - 40 39 40 40 40 40 31 40 40 40 35 40 40 40 40 40 40 40 36 39 40 39 40 39 40 - 40 39 40 40 40 40 40 37 39 40 40 39 38 40 38 39 36 40 40 40 37 39 40 38 39 - 38 35 39 39 39 34 38 36 34 34 35 36 38 39 35 36 35 38 36 33 33 33 36 35 38 - 35 33 38 34 34 34 30 32 31 28 33 31 31 23 33 31 33] ->L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 36 39 34 35 - 31 38 35 39 33 39 39 39 39 36 38 38 36 36 32 34 36 31 36 34 36 34 34 33 35 - 33 27 35 28 35 31 31 32 29 23 32 32 32 32 20 31 23 24 30] ->L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 38 40 40 40 39 39 40 40 40 40 40 40 40 40 40 40 40 - 36 40 40 40 36 40 40 40 40 40 39 40 40 39 39 39 39 39 39 39 39 40 40 38 39 - 36 39 40 40 40 39 40 39 37 39 40 34 38 39 36 37 36 40 36 40 39 36 39 31 28 - 33 35 31 35 36 37 36 34 33 25 33 33 33 34 33 34 37 34 33 33 34 28 34 34 34 - 31 23 36 31 34 33 33 34 33 33 33 33 33 35 23 35 31 26 32 32 31 30 30 33 35 - 35 36 38 35 33 32 32 35 28 29 31 31 31] ->L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[39 39 39 39 39 39 39 39 35 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 37 36 - 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 38 38 39 38 - 39 38 35 39 39 39 37 38 38 38 39 39 39 39 39 39 37 39 38 39 38 39 38 34 36 - 36 17 36 33 27 32 35 31 36 36 36 33 33 36 38 32 38 36 34 36 33 33 23 16 30 - 26 30 30] ->L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 39 40 38 - 39 40 40 40 39 40 40 40 40 40 39 40 40 38 39 40 40 40 40 39 40 40 40 39 40 - 40 38 40 40 40 39 40 39 40 40 40 39 39 40 38 40 39 40 39 40 40 38 40 35 34 - 33 40 36 37 39 39 37 39 35 38 39 38 36 35 33 36 38 34 36 35 34 35 36 34 33 - 33 33 36 31 35 35 31 33 32 29 32 30 34 33 31 33 31 31 33 35 33 33 33 28 33 - 31 28 33 33 33 33 33 33 35 22 33 33 33] ->L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[38 38 38 38 38 38 38 38 35 38 38 38 38 39 39 39 39 39 39 37 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 36 39 39 39 - 39 39 39 39 39 36 39 37 36 37 33 38 38 39 38 38 39 39 33 36 38 36 38 37 39 - 33 36 39 39 33 39 38 39 39 35 39 39 39 38 39 38 33 39 34 23 29 23 30 36 34 - 35 36 36 36 36 34 27 33 34 36 36 33 31 24 16 26 28 28 33 33 25 33 31 33 33 - 29 33 31 14 25 25 25 26 20 28 28 29 29 32] ->L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 39 - 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 37 38 39 39 39 40 38 40 38 39 - 39 40 40 40 40 40 40 39 40 40 39 39 40 40 38 37 37 40 39 39 39 38 40 34 39 - 38 39 38 39 38 38 39 36 35 39 39 38 35 37 38 38 38 38 38 38 35 33 31 33 34 - 34 33 35 32 33 36 31 36 31 33 33 33 33 36 36 33 37 31 30 32 29 30 28 28 33 - 31 14 28 30 31 30 29 26 31 33 33 31] ->L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 38 39 40 40 40 40 35 40 40 40 40 40 - 38 40 40 40 37 40 40 40 38 40 40 40 40 40 40 40 40 34 38 38 40 40 37 33 35 - 38 38 31 40 37 37 40 37 39 38 39 40 39 35 33 36 34 36 39 38 39 38 37 37 35 - 23 37 34 38 34 38 38 38 38 37 38 36 33 35 36 36 36 33 36 36 38 36 38 33 33 - 29 33 33 31 33 33 34 34 32 28 26 30 30 28 27 30 26 19 29 32 31 28 30 25 29 - 31 30 30 25 31 31 7 31] ->L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 40 40 - 39 38 40 40 40 40 40 40 40 40 38 40 40 40 37 40 39 40 40 39 40 40 40 38 40 - 39 37 40 40 39 40 40 39 40 38 39 38 38 39 36 39 27 39 38 39 38 36 37 33 34 - 29 36 33 36 36 36 37 34 38 36 38 35 33 36 36 34 36 33 33 34 34 27 31 33 35 - 33 33 36 35 33 35 33 35 31 33 34 33 34 35 35 35 32 35 33 31 28 28 31 30 31 - 33 28 31 31 33 18 33 28 31 31] ->L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[39 39 39 39 39 39 39 39 39 39 35 39 37 39 39 39 39 39 39 39 39 39 39 39 39 - 38 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 38 - 38 38 29 39 39 39 39 39 36 35 38 35 35 38 37 38 38 38 35 38 38 33 38 36 36 - 38 33 38 38 23 38 31 35 36 38 38 36 37 36 37 36 27 38 38 36 36 37 36 38 38 - 39 38 18 35 38 33 33 33 35 35 38 35 31 38 37 35 36 36 35 33 35 33 33 29 33 - 33 33 35 33 33 35 31 29 30 25 26 30 30 28 30 33] ->L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[39 39 39 38 38 38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 - 39 39 39 38 38 16 35 27 30 32 26 28 26 38 37 38 33 38 38 27 38 35 38 35 35 - 38 38 33 32 32 35 38 31 39 39 33 39 37 38 39 36 38 36 27 30 28 23 23 35 35 - 35 32 35 31 31 33 33 17 25 26 30 31 30 33 29 29 35 26 32 24 32 23 34] ->L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[39 39 39 39 31 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 38 39 - 39 39 39 39 39 39 39 39 39 39 39 38 36 39 39 39 37 39 36 38 36 35 38 35 35 - 35 38 38 37 37 37 35 35 32 33 36 36 36 35 35 34 37 38 36 36 35 33 34 32 36 - 33 36 36 36 36 36 31 36 33 33 36 33 33 36 36 36 36 23 33 31 31 25 27 23 28 - 28 27 28 33 32 32 29 32 31 25 31 23 31] ->L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 - 39 40 40 40 40 40 40 40 40 40 40 40 39 40 39 37 40 40 39 40 40 39 40 39 39 - 39 40 40 40 39 39 40 39 39 40 40 40 39 40 38 40 39 40 37 39 40 37 39 36 35 - 33 40 35 37 37 36 39 39 38 38 39 39 36 37 36 33 40 36 37 37 34 36 36 35 29 - 36 35 36 33 36 36 35 33 36 36 39 35 33 36 37 36 33 35 38 36 36 35 35 33 37 - 36 35 36 33 34 33 33 33 26 34 33 33 33] ->L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 - 39 40 40 40 37 40 40 40 39 40 39 40 40 39 39 40 39 39 40 40 40 40 40 39 37 - 40 40 38 40 37 35 32 38 40 40 39 37 39 39 36 39 31 40 39 40 40 38 40 39 38 - 38 40 36 39 38 39 36 39 38 39 37 39 38 36 36 36 40 39 39 36 36 36 31 36 36 - 36 36 40 36 37 39 33 36 35 37 38 35 37 38 38 36 36 35 36 36 33 33 16 23 32 - 31 31 31 33 31 23 23 31] ->L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 39 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 38 40 40 40 40 38 40 40 - 40 38 40 40 40 39 40 40 40 40 40 39 39 39 40 40 40 39 40 40 40 39 40 39 39 - 40 27 39 37 40 37 39 36 40 39 36 39 38 38 39 34 33 39 33 38 27 35 35 35 32 - 30 31 32 35 31 23 28 33 32 32 30 27 21 30 32 32 30 30 16 27 27 24 26 17 20 - 19 21 27 30 10 25 31] ->L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 25 35 38 38 38 38 37 38 40 40 40 40 39 40 40 40 40 39 - 37 40 40 40 35 40 40 40 40 40 39 40 40 40 39 35 40 40 40 34 40 40 40 36 39 - 40 39 40 40 40 39 40 40 40 39 40 40 39 37 36 36 29 40 40 39 39 38 40 38 36 - 29 37 36 36 36 37 39 36 36 38 38 38 38 34 39 36 39 38 38 36 38 35 36 36 38 - 38 31 38 35 35 36 35 38 36 33 36 38 35 33 36 27 36 33 36 33 33 33 34 31 31 - 31] ->L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 39 40 40 35 40 40 40 40 40 40 36 40 40 40 40 39 - 37 39 40 40 40 39 40 40 40 40 39 37 40 40 40 39 40 40 40 40 40 40 40 36 39 - 38 40 35 40 40 35 40 38 40 37 37 33 40 40 39 36 35 40 36 36 36 37 38 36 39 - 36 40 33 37 34 37 35 36 38 39 38 38 38 39 38 39 38 36 29 36 36 35 36 33 36 - 33 33 35 35 34 34 35 35 34 29 35 34 34 31 34 30 34 33 34 36 31 33 33 33 33 - 29 24 31 27 29 30 26 31 10 26 27 30] ->L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 - 39 40 40 40 39 40 40 40 40 40 39 40 40 40 40 40 39 39 39 39 40 40 40 39 39 - 40 40 38 40 39 40 40 40 39 39 40 40 40 39 34 40 39 40 37 39 40 37 37 36 36 - 31 39 35 39 38 39 37 38 38 38 38 38 36 38 36 36 39 36 38 39 36 34 38 36 36 - 38 38 39 33 33 35 35 33 33 23 33 31 33 31 31 32] ->L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 17 38 38 - 35 38 38 40 39 40 40 40 40 39 40 40 40 40 39 40 39 40 40 36 40 40 40 40 37 - 40 40 40 40 40 39 40 40 40 40 40 40 38 40 37 37 35 40 40 40 40 39 40 36 36 - 35 40 36 40 36 37 39 39 33 36 40 34 36 33 35 34 36 33 36 34 35 36 36 36 39 - 36 33 37 33 34 36 33 35 33 28 33 33 35 30 32 32 30] ->L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 39 40 40 40 38 38 38 38 38 40 39 40 40 40 40 39 33 40 40 40 39 - 40 40 40 40 40 40 40 40 40 38 40 40 39 39 39 39 40 40 40 38 40 40 35 40 39 - 40 40 40 40 39 39 40 37 36 40 36 36 36 37 35 34 37 37 38 36 38 35 39 38 38 - 36 38 38 38 38 39 38 36 36 35 38 38 36 36 35 35 38 37 23 36 33 33 34 35 36 - 36 35 33 33 36 31 31 31 33 34 33 34 33 33 31 36 33 33 33 33 31 28 28 19 31 - 30 30 18 28 28 28 24 24 31 16 33 31] ->L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 38 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 - 35 40 40 40 40 40 40 40 40 40 39 40 40 38 38 36 38 38 40 39 40 40 40 40 40 - 39 40 36 40 40 40 36 40 40 40 39 38 40 40 39 40 40 38 40 38 40 36 40 39 39 - 40 39 39 39 38 36 38 36 34 27 36 39 34 38 38 39 36 37 36 27 36 33 31 33 33 - 36 29 34 34 36 33 29 33 26 14 28 30 28 33 34 33 34 33 29 33 33 29 31 31 33 - 27 31 30 27 23 20 9 24 17 23 20 22 23 28 31 33] ->L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 37 39 39 39 39 39 - 37 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 36 39 39 39 36 36 - 38 35 38 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 35 38 - 38 39 38 39 39 39 39 37 36 39 38 38 33 39 33 36 39 36 38 39 38 39 38 38 39 - 38 35 39 32 36 39 36 39 33 33 35 33 33 33 33 34 29 33 36 36 36 33 33 32 36 - 32 32 36 36 33 36 32 33 29 27 31 33] ->L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[39 39 39 39 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 - 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 35 39 39 39 39 36 37 39 39 39 39 39 31 39 39 39 39 39 36 39 39 36 38 36 36 - 36 39 35 39 36 37 39 36 38 39 36 31 36 39 38 38 39 38 34 31 33 23 35 36 36 - 36 34 36 33 36 36 35 23 26 30 28 30 30 35 33 34 35 33 34 34 34 33 35 33 33 - 27 33 33 33 34 36 23 36 18 28 18 28 30] ->L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 37 39 39 39 39 38 38 - 39 37 39 39 39 39 39 39 39 39 39 39 39 39 38 39 38 39 36 39 39 36 39 35 29 - 34 36 34 38 38 39 39 31 38 38 34 37 38 38 36 38 39 38 33 31 33 33 33 36 36 - 36 36 39 31 33 33 34 34 33 31 33 29 34 36 27 34 31 33 33 36 33 33 32 35 36 - 32 32 29 28 32 31 31 28 25 28 28 30] ->L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[36 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 38 35 38 38 38 38 38 38 38 38 38 38 37 38 37 38 38 38 38 35 38 37 38 38 37 - 29 36 38 36 38 36 37 36 35 23 35 38 35 36 36 36 33 38 35 38 33 35 37 35 33 - 35 33 35 36 36 27 33 36 35 31 31 32 31 23 33 26 28 24 29 23 29 10 29 26 26 - 19 24 26 30] ->L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 39 40 35 40 40 40 40 40 35 40 40 40 40 40 40 40 40 40 40 38 - 35 40 40 40 36 40 40 40 40 40 40 40 40 37 37 37 40 40 40 39 40 40 40 39 36 - 40 40 40 40 38 37 40 40 40 38 40 40 39 40 36 39 36 40 37 35 40 37 40 38 38 - 38 39 36 40 36 35 39 34 38 39 38 39 39 35 36 36 40 36 36 36 36 36 36 38 38 - 38 29 38 35 36 35 33 33 29 32 35 32 35 31 32 26 29 19 27 28 27 24 21 22 25 - 27 27 28 25 30] ->L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 - 39 38 39 39 39 39 39 39 39 39 36 39 39 38 38 33 38 38 38 39 39 39 39 39 39 - 39 39 39 39 38 39 39 39 39 39 39 38 36 38 37 37 38 37 37 38 37 35 37 36 37 - 39 37 35 39 36 36 39 33 34 17 33 36 34 23 28 30 30 32 33 33 33 31 35 31 31 - 29 33 33 33 33 31 20 31 22 22 28 28 16 34 34 31 33 33] ->L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[38 38 38 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 38 40 40 40 40 40 - 35 40 40 40 38 40 40 40 40 40 40 40 39 40 40 40 38 40 40 38 40 40 39 36 38 - 38 38 34 40 40 37 40 40 40 39 40 40 40 40 39 38 39 40 39 39 40 36 39 39 36 - 35 35 39 37 33 40 35 37 37 39 39 37 36 38 38 38 39 38 29 36 36 33 36 33 34 - 36 33 34 31 33 33 33 33 28 21 24 28 11 25 16 23 21 25 36 33 36 33 34 26 26 - 31 25 31] ->L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 39 40 40 40 40 40 40 39 - 39 40 40 40 40 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 38 - 39 39 40 40 40 39 39 40 39 40 40 40 39 40 36 36 36 40 39 40 40 38 40 37 34 - 35 40 33 39 39 40 31 39 39 38 36 35 35 29 33 31 35 33 36 33 33 33 36 36 36 - 33 36 37 34 36 38 36 36 33 27 35 33 33 36 36 33 36 33 31 33 33 27 34 28 33 - 22 31 33 31 34 33 31] ->L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 35 40 40 40 40 40 40 40 39 40 39 40 40 38 40 40 38 40 40 39 40 40 40 40 40 - 40 39 39 40 33 40 40 40 40 40 40 40 38 40 36 40 39 38 40 40 40 36 39 40 40 - 31 40 38 40 39 37 35 39 40 38 35 40 39 39 37 36 36 40 38 33 33 27 35 35 37 - 37 37 37 36 37 35 36 33 33 35 38 38 33 37 34 36 36 34 35 35 35 31 35 31 37 - 33 33 35 35 26 33 33 32 32 31 35 24 35 25 25 30 18 28 27 21 20 23 20 20 26 - 25 27] ->L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[39 39 39 38 36 38 38 38 37 38 33 38 38 39 39 39 39 39 39 37 34 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 35 39 36 37 37 37 38 31 36 36 36 37 27 39 37 - 39 39 39 39 35 39 37 23 35 39 33 39 39 39 32 38 39 38 37 37 35 32 35 35 35 - 37 34 33 36 36 35 35 32 34 36 33 36 33 36 33 22 29 36 29 29 8 29 31 36 35 - 34 34 31 33 27 29 25 20 25 21 25 29 21 23 29 15 32 26 24 29 26 25 31 30 27 - 29 30 30 31 33] ->L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 - 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 - 39 39 39 39 38 33 39 39 39 39 39 33 39 39 39 39 39 39 39 37 39 38 39 33 39 - 39 37 38 36 38 36 36 33 36 39 39 39 35 35 35 33 31 38 33 28 30 28 30 36 36 - 36 29 36 33 35 18 28 31 32 32 33 35 35 35 29 33 33 34 32 27 32 28 32] ->L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 39 40 40 37 40 40 40 38 38 39 40 40 39 40 35 40 39 40 40 38 - 33 40 40 40 36 40 40 40 39 39 36 40 40 39 36 38 39 39 39 36 39 38 40 35 33 - 36 38 33 40 37 36 37 37 36 36 39 36 33 39 36 37 34 39 38 38 39 36 39 36 31 - 33 39 33 37 35 37 36 36 38 36 35 35 36 33 33 33 39 36 31 35 33 33 35 33 36 - 33 33 36 35 36 36 35 33 36 29 32 36 34 35 34 35 33 31 33 33 31 25 34 31 33 - 33 31 33 28 15 28 15 29 30 28 29 31] ->L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 - 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 39 40 37 40 40 40 40 40 39 39 - 40 37 40 40 40 39 38 39 39 40 39 40 40 40 39 39 40 40 37 37 40 34 40 33 23 - 33 35 38 35 31 35 33 32 34 37 36 35 38 34 36 36 33 37 37 37 34 33 33 33 36 - 33 36 33 32 32 26 23 30 29 30 32 32 32 33 33 34 34 31 33 33 29 29 34 31 35 - 33 31 33 31 22 31 31 31 23 18 26 23 31 23 24 27 22 10 28 23 28 7 28 20 30 - 24 30] ->L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[27 31 31 38 38 38 38 38 38 38 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 38 38 38 38 38 38 35 36 38 38 38 38 38 38 38 38 38 38 38 33 38 38 38 38 38 - 38 35 38 38 38 33 27 33 38 38 38 38 38 38 38 38 38 38 29 27 38 38 38 35 38 - 35 38 38 38 36 38 35 38 38 38 38 38 36 38 37 36 27 35 38 35 35 35 35 38 38 - 38 33 38 35 35 38 35 25 33 33 35 35 35 30 30 25 27 30 38 38 35 33 33 26 33] ->L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 38 40 40 40 40 38 39 40 40 38 38 40 40 40 40 40 39 40 40 40 40 39 - 40 39 40 40 40 39 40 40 40 40 36 40 36 40 37 37 38 38 38 40 37 40 39 40 40 - 39 37 40 39 40 39 35 37 40 40 40 38 36 39 36 39 39 40 39 40 38 38 37 36 38 - 38 27 39 36 38 33 35 29 31 29 32 35 32 36 36 36 31 35 30 32 28 27 28 31 33 - 31 31 31 33 31 33 29 33 33 33 33 34 32 31 31 8 29 31 27 19 29 16 29 31 31 - 29 26 29 32 19 25 31 32] ->L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 - 35 40 40 40 40 40 40 40 40 40 39 40 40 39 40 40 40 40 40 39 40 40 40 39 35 - 40 37 40 40 40 40 40 40 40 40 40 40 39 40 38 40 38 40 39 39 40 36 40 37 37 - 36 40 36 40 39 40 37 40 38 38 39 38 39 35 39 39 39 38 39 38 33 27 36 36 35 - 39 36 37 38 38 39 33 38 36 35 39 39 39 33 34 33 36 33 36 36 35 31 33 23 31 - 31 31 32 31 33 31 31 31 23 30 18 30] ->L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 39 - 38 40 40 40 39 40 40 40 40 40 40 40 40 40 39 39 40 40 40 40 40 38 37 40 40 - 40 40 40 40 40 40 40 40 40 40 40 39 38 40 37 37 38 40 37 40 40 37 40 38 39 - 33 40 39 38 40 39 40 38 39 38 38 36 35 35 33 35 33 37 35 29 33 31 31 33 33 - 33 28 33 33 35 33 30 31 33 25 33 33 33 36 36 36 34 33 30 30 32 19 31 19 30 - 28 28 30] ->L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[28 33 31 38 35 38 38 38 36 38 36 38 38 38 38 38 38 38 38 36 38 38 38 38 38 - 35 38 38 38 38 36 36 36 38 38 38 33 38 38 38 38 38 38 38 35 38 38 38 38 38 - 38 38 31 36 36 33 33 36 30 30 30 35 33 38 38 38 36 38 33 38 38 35 38 38 38 - 38 33 34 30 15 30 27 33 37 38 37 29 33 35 38 35 35 38 34 33 35 35 35 35 35 - 35 35 35 36 38 38 37 35 35 35 32 38 10 7 17 25 18 24 35 33 32 32 32] ->L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 37 - 37 40 40 40 37 40 40 40 40 40 39 40 40 40 39 36 40 40 40 39 40 40 40 39 39 - 40 37 40 40 40 40 39 40 40 40 39 39 39 40 38 39 39 37 39 40 39 36 40 35 33 - 33 37 38 39 37 39 37 36 39 36 40 40 36 33 35 33 36 36 35 34 35 36 37 28 35 - 33 35 35 34 33 35 28 35 31 33 35 36 33 35 37 34 34 29 33 31 33 19 33 28 31 - 28 29 31 28 33 33 29 23 30] diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/sequence_qualities.qual --- a/test-data/split_libraries_fastq/sequence_qualities.qual Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,584 +0,0 @@ ->L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[32 34 34 34 38 38 38 38 31 38 30 38 38 38 38 38 38 38 38 35 38 38 38 38 38 - 38 38 38 38 38 38 38 38 38 38 38 35 38 38 33 38 38 38 33 38 38 38 38 38 38 - 38 35 38 36 38 38 35 35 38 31 38 38 38 38 36 36 36 38 38 38 38 38 38 18 23 - 23 38 35 29 35 35 38 38 36 36 31 35 35 35 35 33 29 37 35 38 35 29 31 12 25 - 22 19 28 26 32 9 22 19 28 28 28 28 30 33 33 35 31] ->L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 37 - 39 33 39 39 36 34 34 39 33 35 39 37 36 39 32 36 31 31 33 35 33 28 33 31 31 - 32 17 35 7 20 20 20 18 35 32 32 32 32 36 31 36 36 32 29 26 8 24 26 28 28 - 32 29 22 33 31 28 31 22 23 26 25 23 17 30 34 27 29 27 24 30 27 29 29 29 24 - 23 28 31] ->L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 38 40 40 40 40 38 40 40 40 40 40 38 40 40 39 40 35 40 39 40 39 39 - 37 31 40 40 37 40 40 40 40 40 38 40 40 40 39 40 39 38 39 33 40 40 40 38 39 - 39 38 40 37 36 36 36 37 39 39 39 35 36 36 35 38 29 35 33 36 37 33 35 33 31 - 33 33 28 36 36 33 33 33 36 38 36 35 35 33 32 29 35 31 31 31 31 33 34 33 33 - 26 33 35 31 31 29 25 31 31 29 33 31 30 28 16 31 31 31 18 27 24 22 21 22 28 - 21 29 26 26 30] ->L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 38 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 40 - 37 40 40 38 36 38 38 35 40 40 40 40 40 37 37 39 40 40 38 35 38 36 38 35 29 - 38 35 38 40 40 36 40 38 40 40 37 39 29 38 33 35 35 38 37 38 38 36 38 33 33 - 17 35 33 35 35 38 33 35 36 33 36 33 33 23 30 27 33 29 33 29 32 31 35 28 18 - 23 28 32 34 29 30 29 31 31 30 35 35 31 29 28 30 28 19 22 23 26 10 26 33 36 - 23 29 33] ->L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 39 40 40 40 40 40 40 39 36 36 40 40 36 40 40 40 40 40 40 38 - 38 39 40 39 36 40 40 39 35 37 34 40 38 39 39 39 34 36 35 33 40 36 40 36 36 - 39 33 33 36 33 33 36 40 39 38 38 38 38 38 35 39 38 38 36 36 38 35 38 30 27 - 17 32 20 35 33 33 33 33 36 33 36 33 33 33 31 33 36 35 36 33 33 33 36 26 31 - 31 31 33 31 31 23 25 31 33 31 31 31 31 32 36 31 31 32 15 31 29 28 27 24 25 - 26 24 28 22 29 25 26 28 28 27 30] ->L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[39 39 39 38 38 36 38 36 38 38 38 38 38 39 39 39 39 39 39 39 38 39 39 38 38 - 34 38 38 39 37 39 39 39 39 39 39 39 39 36 35 33 38 38 39 37 39 39 39 39 37 - 39 39 39 39 39 37 39 39 39 39 39 36 33 37 34 36 36 35 37 37 37 35 36 28 26 - 31 34 28 34 34 34 32 32 33 33 35 31 31 25 28 26 30 28 33 33 31 29 35 33 35 - 28 31 35 33 33 33 28 35 21 6 28 26 26 32 28 30 28 24 24 28 24 13 24 13 17 - 11 14 17 28 28 28 22 24 31 31 21 31] ->L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 40 40 40 38 40 40 40 35 38 40 40 40 37 40 40 39 40 40 40 40 - 40 40 40 40 40 40 40 40 40 40 36 40 37 38 37 38 38 38 39 40 40 38 40 40 40 - 39 33 39 40 39 38 40 38 40 39 40 31 36 38 31 36 38 37 37 40 37 36 35 37 33 - 34 27 37 36 34 31 33 10 31 33 33 33 33 23 17 28 28 30 33 33 31 27 33 33 31 - 33 33 8 32 32 31 23 28 31 30 31 31 20 30 27 29 28 26 26 17 30 12 30 28 32 - 30] ->L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 33 40 40 40 38 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 - 40 40 39 38 40 40 40 40 40 40 40 36 35 38 37 38 36 35 35 38 35 33 38 25 31 - 31 36 34 38 36 37 36 33 39 33 39 37 39 33 33 31 31 38 38 33 33 33 35 33 36 - 34 33 33 38 36 34 33 36 34 33 37 36 33 35 36 33 36 33 33 31 31 17 33 19 30 - 27 28 30 26 32 30 30 32 31 32 10 29 30] ->L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 39 38 38 40 40 39 40 40 40 40 40 40 40 - 39 40 40 40 39 40 40 40 40 40 38 40 39 39 40 40 40 40 39 35 40 40 40 39 39 - 39 35 40 40 40 39 40 40 39 35 39 38 36 39 36 39 38 35 38 39 36 35 38 35 39 - 36 36 37 36 39 38 11 36 35 34 35 34 37 31 36 33 33 36 34 33 31 31 33 21 29 - 31 32 31 36 34 33 33 34 33 33 36 36 32 29 33 33 33 31 31 35 33 32 33 28 31 - 23 31 31] ->L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 38 39 39 39 39 38 39 39 39 39 38 33 39 39 39 39 39 - 39 39 39 39 37 39 39 39 39 36 39 39 39 39 36 37 36 39 37 38 38 37 38 32 32 - 29 34 28 38 38 35 33 35 36 35 36 36 33 34 33 33 36 36 33 33 29 33 35 31 33 - 33 31 35 33 33 33 33 28 34 29 33 34 34 33 29 36 33 33 23 30] ->L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 38 33 38 38 38 36 38 38 38 38 31 38 38 35 38 39 39 - 37 38 39 39 39 39 39 39 38 38 37 36 38 33 38 38 38 18 34 36 29 36 32 32 33 - 38 35 33 37 37 36 35 37 36 29 36 29 35 34 33 31 36 37 37 33 34 29 36 35 33 - 35 35 10 34 32 32 29 29 24 30 28 24 24 35 34 34 30 32 34 32 33 36 33 33 36 - 33 27 33 33 35 31 33 31 31 31 33] ->L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 - 40 40 40 40 40 40 40 40 40 40 39 40 40 38 40 40 38 40 40 40 40 39 40 40 40 - 40 35 38 40 33 37 39 35 39 33 36 39 39 40 36 35 39 38 39 39 40 37 37 38 38 - 39 31 29 36 36 34 36 33 38 35 36 34 35 36 36 36 36 36 36 35 23 33 29 31 31 - 31 30 30 36 36 33 9 33 22 16 26 17 29 22 31 30 28 28 28 31] ->L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 - 33 40 40 40 37 40 40 40 40 40 39 40 40 39 39 39 40 40 37 37 40 40 40 37 36 - 40 37 40 40 40 39 40 40 40 40 40 39 38 40 36 36 34 39 36 37 40 35 40 33 34 - 29 38 33 37 37 37 36 36 39 33 36 38 36 33 33 36 37 35 33 34 33 33 37 23 32 - 30 30 32 33 33 35 28 33 31 31 35 33 33 30 30 32 26 26 29 30] ->L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 38 40 - 37 40 40 40 40 40 40 40 40 40 39 40 40 40 39 39 40 39 39 37 40 40 40 38 40 - 40 40 36 38 40 37 39 40 40 38 39 40 40 40 38 39 38 40 38 40 38 39 40 36 35 - 33 37 32 36 39 39 37 36 38 38 35 38 36 27 34 33 37 34 38 35 36 38 38 38 38 - 36 38 39 36 36 34 36 29 34 33 34 31 33 35 33 31] ->L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 35 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 37 38 38 38 37 38 - 38 36 38 38 38 38 38 38 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 36 36 - 36 38 34 39 39 39 38 38 38 39 39 39 33 39 38 39 29 36 36 35 33 35 36 39 39 - 39 35 37 33 37 38 29 27 30 30 34 32 34 23 35 35 32 33 35 36 36 33 36 35 33 - 31 33 36 33 31 33 29 33] ->L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 40 - 36 40 40 40 39 40 40 40 38 40 40 40 40 40 40 39 40 39 37 40 40 40 40 40 35 - 40 39 39 40 38 38 40 40 40 40 39 33 39 40 37 40 37 40 40 40 39 39 40 36 36 - 31 38 33 38 35 35 35 35 31 36 39 36 36 36 36 34 33 36 36 36 35 34 37 33 34 - 33 33 37 36 36 40 29 31 33 31 35 33 35 33 36 36 33 24 30 30 30 11 30 27 32 - 29 31 32 28 32 28 27 28 26 9 26 30] ->L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 35 40 40 40 40 40 - 39 40 40 40 39 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 39 - 37 39 38 40 39 39 40 38 38 40 36 39 35 40 34 35 36 40 36 40 40 36 40 33 33 - 33 35 31 37 33 36 36 33 36 36 36 37 35 35 33 36 37 36 33 33 33 33 36 33 33 - 33 33 35 30 33 33 23 23 27 25 27 24 30 33 31 33 31 31 31 32 30 18 31 31 31 - 24 30 31] ->L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[39 39 39 39 39 39 39 39 35 32 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 31 39 39 39 39 38 39 39 - 37 38 39 39 39 39 39 39 39 37 39 39 39 39 38 39 39 39 35 39 39 38 39 38 38 - 39 27 38 36 31 35 36 33 36 37 36 37 39 38 38 38 33 38 31 33 33 36 35 33 35 - 33 31 35 36 33 37 36 36 38 36 36 29 38 33 34 29 35 36 34 36 35 33 29 21 29 - 29 29 32 21 29 29 26 19 29 28 21 25 28 24 28 33 25 31] ->L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 36 39 39 39 39 39 39 39 37 39 39 39 36 39 36 39 39 39 39 39 39 39 33 - 39 39 36 39 38 39 39 39 37 38 38 38 38 33 36 36 39 36 38 34 33 38 35 34 38 - 36 38 38 36 38 38 36 38 36 34 34 36 34 31 36 36 29 33 31 32 28 28 31 22 11 - 23 28 29 26 16 28 24 32 29 29 29 26 32] ->L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 38 40 39 39 40 40 40 40 40 38 40 40 40 40 40 - 37 40 40 40 39 40 40 40 40 40 39 40 40 40 39 40 40 38 40 38 40 40 40 39 40 - 40 37 40 40 40 38 40 40 40 35 39 38 35 40 37 40 37 36 39 40 37 33 39 33 35 - 37 37 37 36 40 36 23 33 35 36 35 35 36 36 37 35 35 39 36 34 34 31 36 32 33 - 34 36 36 33 32 35 18 35 31 33 34 34 34 36 37 29 34 33 33 33 34 23 34 30 33 - 31 28 31 31 27 27 29 33 33 28 31 31 33] ->L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[38 38 38 39 39 39 39 39 38 36 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 38 39 39 39 39 39 33 39 39 39 39 39 39 38 34 38 33 38 36 36 - 38 36 36 38 38 35 33 33 32 35 33 38 35 37 35 36 36 38 34 38 37 36 37 37 34 - 35 33 33 35 33 36 34 34 36 37 39 36 36 36 36 25 39 33 26 32 27 25 25 28 30 - 29 28 26 36 36 36 32 36 31 29 32 34 29 11 25 13 20 17 30 26 25 20 17 20 20 - 25 11 22 31 18 25 19 25 31] ->L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 38 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 38 40 38 40 40 39 - 40 40 40 37 40 39 40 40 40 40 39 39 40 40 40 40 40 36 40 36 40 39 40 35 35 - 38 38 31 38 38 33 38 38 38 39 40 40 40 40 38 40 37 40 38 40 40 39 39 36 36 - 36 38 33 39 36 39 39 37 37 38 39 38 38 38 38 36 38 35 36 33 36 35 36 32 34 - 35 35 34 32 33 33 35 35 33 31 31 31 33 36 33 36 33 34 34 33 35 33 33 33 33 - 35 36 36 30 33 31 29 33] ->L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[38 38 38 39 39 39 39 39 33 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 38 39 39 39 39 39 38 39 39 39 39 39 33 38 39 39 39 39 39 38 - 39 39 33 38 38 36 38 36 35 38 33 38 38 39 39 39 29 39 35 33 38 35 38 39 39 - 38 39 36 35 29 38 33 31 38 36 38 36 38 39 37 33 39 36 35 31 33 35 35 35 39 - 37 35 31 34 29 37 34 36 37 36 39 33 33 29 31 30 25 29 30 30 23 23 17 21 28 - 23 14 25 26 27 27 25 30 29 8 29 32] ->L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 33 39 39 40 40 39 40 39 40 40 40 40 40 - 34 40 40 40 37 40 40 40 40 40 39 40 40 40 39 38 39 39 37 36 40 39 40 36 37 - 40 40 33 40 39 37 39 40 40 39 40 40 38 39 36 39 38 39 38 39 39 38 39 35 33 - 33 40 34 39 36 37 39 36 38 38 35 38 36 36 34 38 39 36 38 35 36 29 38 38 36 - 35 36 38 35 35 35 33 36 36 33 36 36 31 33 33 36 33 33 35 38 35 34 28 32 33 - 33 33 33 30 28 28 12 25 28 28 30] ->L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 40 35 40 40 40 18 40 40 38 38 38 38 38 40 40 40 40 40 31 39 40 40 38 40 40 - 39 38 40 40 40 40 40 39 40 40 39 40 40 40 39 40 40 35 36 38 38 36 38 38 39 - 39 40 39 40 39 40 40 38 35 37 40 33 37 27 35 33 35 35 33 40 35 39 39 40 40 - 38 38 38 37 40 37 36 39 32 32 34 36 32 36 40 35 40 24 40 40 37 34 37 37 36 - 36 36 37 27 33 33 27 35 28 28 30 30 30 27 31] ->L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 40 40 40 - 38 40 40 40 39 40 40 40 40 40 38 40 39 39 39 39 39 39 40 39 40 39 40 35 36 - 40 38 34 40 37 39 39 40 39 36 40 39 36 39 36 39 36 40 36 40 39 36 40 33 35 - 33 37 33 37 34 35 35 35 38 36 38 36 36 35 35 35 38 34 33 35 33 33 35 32 33 - 36 33 36 35 38 35 36 32 36 33 34 33 34 33 33 36 33 33 31 36 33 22 33 33 31 - 33 33 33 21 30] ->L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[34 34 34 38 33 38 38 38 36 38 38 38 38 38 38 38 36 38 38 38 38 38 38 38 38 - 35 38 38 38 38 38 36 35 38 38 37 38 38 38 38 38 38 38 38 37 35 35 38 37 38 - 38 36 38 38 38 33 36 38 36 38 35 38 38 38 37 38 29 38 37 38 38 37 38 37 36 - 36 38 36 37 36 37 31 36 36 36 36 37 36 33 36 35 37 35 34 35 31 33 33 36 35 - 36 29 36 35 34 37 33 36 35 33 36 37 29 26 32 29 25 29 32 32 23 29 29 26 32 - 32] ->L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 37 40 39 40 40 40 40 38 40 40 40 40 40 - 37 40 40 40 39 40 40 40 40 40 40 40 40 40 40 35 40 39 40 40 40 39 40 38 40 - 40 39 40 40 40 40 40 40 40 40 37 39 39 40 38 39 38 40 39 40 38 36 35 33 33 - 33 38 33 38 39 37 39 38 34 38 39 38 36 33 36 36 40 36 36 33 36 36 34 36 36 - 39 34 39 33 36 37 33 33 33 31 33 33 33 34 29 36 31 33 30 31 31 30 31 28 31 - 31 33 33 26 29 31 30 29 31 30 29 32] ->L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[35 38 38 36 33 36 36 36 30 33 11 33 30 38 38 38 38 38 38 38 38 38 38 38 38 - 38 38 38 38 38 29 38 38 35 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 38 38 38 38 38 32 29 29 38 31 29 38 27 36 30 33 36 36 31 38 38 35 38 38 35 - 38 33 35 38 36 33 29 35 35 38 35 38 37 37 38 35 23 37 33 33 30 30 29 38 35 - 38 35 33 32 31 32 35 26 32 32 32 36 20 30 33 26 26 19 32 35 35 33 33 33 31 - 33 31 35 16 13 30 23 30 30 12 23 25 25 26 30] ->L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 40 40 39 40 - 33 40 40 40 37 40 40 40 40 40 39 40 40 40 40 40 40 36 37 40 40 37 40 36 39 - 37 39 39 40 40 37 40 40 40 40 39 40 39 40 36 40 39 40 39 40 40 37 40 34 36 - 34 40 33 39 39 40 37 36 39 35 36 38 36 34 33 33 39 36 35 34 33 35 37 34 36 - 36 33 39 33 33 36 34 29 33 28 33 33 33 34 36 33 35 32 31 33 33 29 33 29 33 - 33 31 34 33 28 30 31] ->L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[40 40 40 40 40 40 40 40 39 40 40 40 35 40 40 40 40 40 40 40 40 40 40 40 40 - 37 40 40 40 39 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 - 40 40 40 40 36 40 40 40 40 40 40 40 38 40 39 39 36 40 39 40 39 39 40 34 36 - 39 40 39 39 40 40 31 36 39 36 39 37 36 38 35 38 39 38 36 37 36 36 37 38 38 - 33 36 39 33 36 36 27 37 31 31 33 35 35 35 34 33 35 33 35 35 33 33 33 29 33 - 33 33 36 33 36 33 31 31 29 31 28 31 33] ->L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 39 40 39 40 36 38 38 38 36 40 40 40 40 40 40 40 40 40 40 40 40 - 38 40 40 40 39 40 40 40 36 38 33 38 35 35 35 35 38 38 38 40 40 40 33 36 39 - 40 40 37 39 39 40 40 37 40 40 38 35 33 34 31 29 36 34 18 31 30 29 31 38 36 - 38 38 31 31 35 33 35 31 36 23 34 34 28 33 33 35 33 36 32 34 11 18 23 28 28 - 25 26 28 31 30 11 18 28 24 12 27 26 27 30 32 30 30] ->L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 - 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 38 36 39 39 39 39 39 - 39 39 39 39 39 38 39 39 39 39 39 39 38 36 33 36 36 38 36 38 38 37 38 34 34 - 33 38 31 36 36 37 35 36 36 36 38 35 36 36 33 33 39 36 35 36 33 36 36 38 38 - 33 36 38 33 32 36 33 35 31 32 39 35 34 30 34 36 31 30 33 35 33 25 29 21 23 - 27 28 28 28 33] ->L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[39 39 39 39 39 39 39 39 36 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 37 39 39 39 39 39 39 39 35 33 38 38 37 39 39 39 39 33 38 38 35 35 35 39 39 - 39 39 38 38 39 39 33 37 38 37 34 38 38 37 33 36 38 38 35 38 35 29 35 29 24 - 32 34 32 34 36 36 36 33 36 36 33 33 34 35 33 31 36 37 29 35 33 33 31 34 18 - 9 28 31 32 32 31 28 31 33 32 36 36 33 34 34 33 31 34] ->L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 39 - 39 40 40 40 39 40 40 39 40 40 39 40 40 40 37 39 40 40 37 39 40 40 40 39 37 - 39 40 40 35 39 39 40 39 40 40 40 40 39 40 36 39 35 40 38 40 39 38 37 35 35 - 34 37 34 33 31 35 36 33 37 36 37 34 37 34 29 36 35 35 33 33 33 33 33 32 32 - 32 30 32 31 33 33 31 36 31 30 29 12 31 32 31 33 30 30 31 35 33 12 32] ->L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 38 39 40 40 40 37 39 - 39 40 39 40 38 40 40 40 40 39 40 40 40 40 38 39 39 40 39 40 38 39 40 36 34 - 34 38 34 37 37 40 37 37 39 35 37 38 33 28 31 31 35 33 33 33 33 33 33 35 33 - 30 31 36 33 33 33 28 33 29 28 33 31 33 33 33 33] ->L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[39 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 39 40 40 40 40 40 40 40 - 33 40 40 40 38 40 40 40 40 40 38 40 38 40 39 40 37 40 39 39 40 40 40 38 39 - 40 40 40 37 39 39 40 38 35 37 40 36 36 40 36 37 40 35 37 39 39 38 36 38 33 - 39 34 37 39 38 36 38 35 38 34 35 36 33 34 34 33 33 36 36 34 29 33 31 33 29 - 33 23 31 33 33 33 25 33 26 30 30 30 24 31 33 29 34 31 29 26 30] ->L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 - 40 40 40 40 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 39 40 39 40 36 40 - 40 37 36 39 39 40 40 40 40 40 40 40 39 40 38 40 39 40 33 35 39 36 39 38 39 - 39 39 38 39 39 36 38 35 33 34 35 37 29 35 35 34 33 37 36 31 30 33 33 31 33 - 31 22 23 33 33 26 31 28 31 31 30 31 28 26 32 28 30 30 30 30 31 25 29 28 31 - 28 33] ->L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 38 40 40 40 39 40 40 40 40 40 39 40 40 39 39 39 40 40 40 39 40 40 40 36 36 - 40 37 40 40 40 37 39 40 40 40 39 39 36 40 38 38 38 40 38 40 40 38 40 38 38 - 35 40 36 38 38 39 38 39 35 36 36 32 33 34 34 36 35 33 34 31 33 33 36 33 33 - 35 33 37 36 36 38 32 33 33 31 33 34 34 27 27 30 6 10 28 29 27 27 26 25 32 - 15 30 32] ->L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 38 40 40 40 40 40 40 40 40 38 38 40 40 40 40 40 40 40 40 40 40 - 40 40 40 40 40 40 40 40 35 40 40 40 40 40 40 40 40 39 40 39 39 39 40 40 40 - 39 37 40 40 39 39 40 38 40 40 40 40 39 38 36 40 35 38 37 40 40 39 40 39 37 - 36 40 36 39 38 40 38 39 38 38 36 38 31 36 36 33 38 36 36 33 35 36 36 36 35 - 38 36 39 39 35 38 39 39 38 35 35 34 38 33 35 33 33 30 35 33 35 31 33 30 30 - 32 23 32 30 31 35 27 33 22 12 30] ->L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[32 29 33 38 38 38 38 38 38 38 35 38 38 38 38 38 38 38 38 31 38 38 38 38 38 - 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 35 38 38 35 38 34 37 - 37 29 30 38 38 38 38 38 38 36 38 38 38 38 38 38 23 38 38 38 38 35 38 38 33 - 38 27 38 36 38 27 38 38 36 35 36 38 38 38 38 36 36 38 16 28 30 30 33 36 37 - 35 29 34 36 29 34 36 34 35 36 33 36 34 30 29 11 10 30 32 28 30 30 28 28 33 - 31 33 33 32 27 29 29 14 28 24 30 23 30 29 29 30] ->L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 38 40 40 40 40 40 - 33 40 40 40 37 40 40 40 38 40 37 40 40 40 39 39 39 39 40 39 40 40 40 37 39 - 40 38 36 40 40 39 39 40 39 39 40 39 29 40 37 40 36 40 39 39 38 39 40 33 34 - 29 38 34 37 36 40 36 36 39 38 36 38 35 36 36 35 38 35 33 34 33 33 33 35 35 - 36 36 39 31 27 33 33 33 36 33 34 33 36 36 37 36 36 33 36 36 36 31 32 25 30 - 31 33 33 27 20 23 6 17 27 27 27 21 24 26 30 32] ->L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[36 37 37 38 38 38 38 38 38 33 35 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 38 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 33 38 - 36 38 38 36 38 31 38 38 38 38 36 37 37 38 36 34 38 38 34 34 36 32 34 35 35 - 35 23 38 26 31 32 31 31 31 33 35 35 33 37 36 39 36 35 33 29 17 31 32 36 36 - 33 29 33 32 32 29 29 24 29 28 32 32 22 22 29 29 31 30] ->L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[33 36 36 38 38 38 38 38 18 36 33 36 35 36 36 36 36 36 38 38 18 27 38 36 36 - 35 27 36 38 38 31 36 35 38 38 33 27 38 36 36 36 36 32 33 13 19 33 33 20 29 - 31 29 11 36 36 27 36 36 38 38 38 38 35 38 37 38 38 38 29 38 38 32 35 33 30 - 30 27 16 21 14 19 20 24 35 37 35 38 37 37 37 35 33 16 20 9 24 25 14 32 32 - 32 32 32 7 30 30 26 26 37 34 34 33 35 35 35 31 29 33 33 35 33 33 33 23 20 - 29 26 32] ->L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[33 33 33 38 38 38 38 38 35 35 38 38 38 32 34 32 31 11 26 27 20 23 26 38 35 - 38 38 38 38 38 38 37 38 38 33 38 38 36 38 38 38 38 38 38 38 38 36 38 36 38 - 36 38 33 38 36 38 38 38 36 38 23 36 36 37 36 37 36 38 37 33 37 32 35 34 34 - 36 33 35 33 37 33 36 36 36 36 32 36 33 33 33 35 35 27 29 32 23 29 29 32 36 - 36 33 36 31 35 33 33 23 35 33 31 33 15 29 24 8 29 29 31 28 23 31 29 25 18 - 25 23 31] ->L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[27 31 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 35 38 38 38 38 38 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 36 38 38 38 38 38 38 33 36 38 38 38 38 38 36 38 38 38 38 38 38 38 38 33 38 - 17 38 33 38 35 38 38 35 36 38 35 38 37 36 38 37 38 36 38 37 37 35 36 36 38 - 35 36 38 36 37 35 36 38 35 35 33 35 37 34 23 37 34 33 35 35 34 34 35 35 33 - 37 33 34 20 32 32 29 32 29 32 27 32 32] ->L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 - 40 39 40 40 40 40 31 40 40 40 35 40 40 40 40 40 40 40 36 39 40 39 40 39 40 - 40 39 40 40 40 40 40 37 39 40 40 39 38 40 38 39 36 40 40 40 37 39 40 38 39 - 38 35 39 39 39 34 38 36 34 34 35 36 38 39 35 36 35 38 36 33 33 33 36 35 38 - 35 33 38 34 34 34 30 32 31 28 33 31 31 23 33 31 33] ->L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 36 39 34 35 - 31 38 35 39 33 39 39 39 39 36 38 38 36 36 32 34 36 31 36 34 36 34 34 33 35 - 33 27 35 28 35 31 31 32 29 23 32 32 32 32 20 31 23 24 30] ->L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 38 40 40 40 39 39 40 40 40 40 40 40 40 40 40 40 40 - 36 40 40 40 36 40 40 40 40 40 39 40 40 39 39 39 39 39 39 39 39 40 40 38 39 - 36 39 40 40 40 39 40 39 37 39 40 34 38 39 36 37 36 40 36 40 39 36 39 31 28 - 33 35 31 35 36 37 36 34 33 25 33 33 33 34 33 34 37 34 33 33 34 28 34 34 34 - 31 23 36 31 34 33 33 34 33 33 33 33 33 35 23 35 31 26 32 32 31 30 30 33 35 - 35 36 38 35 33 32 32 35 28 29 31 31 31] ->L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[39 39 39 39 39 39 39 39 35 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 37 36 - 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 38 38 39 38 - 39 38 35 39 39 39 37 38 38 38 39 39 39 39 39 39 37 39 38 39 38 39 38 34 36 - 36 17 36 33 27 32 35 31 36 36 36 33 33 36 38 32 38 36 34 36 33 33 23 16 30 - 26 30 30] ->L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 39 40 38 - 39 40 40 40 39 40 40 40 40 40 39 40 40 38 39 40 40 40 40 39 40 40 40 39 40 - 40 38 40 40 40 39 40 39 40 40 40 39 39 40 38 40 39 40 39 40 40 38 40 35 34 - 33 40 36 37 39 39 37 39 35 38 39 38 36 35 33 36 38 34 36 35 34 35 36 34 33 - 33 33 36 31 35 35 31 33 32 29 32 30 34 33 31 33 31 31 33 35 33 33 33 28 33 - 31 28 33 33 33 33 33 33 35 22 33 33 33] ->L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[38 38 38 38 38 38 38 38 35 38 38 38 38 39 39 39 39 39 39 37 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 36 39 39 39 - 39 39 39 39 39 36 39 37 36 37 33 38 38 39 38 38 39 39 33 36 38 36 38 37 39 - 33 36 39 39 33 39 38 39 39 35 39 39 39 38 39 38 33 39 34 23 29 23 30 36 34 - 35 36 36 36 36 34 27 33 34 36 36 33 31 24 16 26 28 28 33 33 25 33 31 33 33 - 29 33 31 14 25 25 25 26 20 28 28 29 29 32] ->L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 39 - 39 40 40 40 40 40 40 40 40 40 39 40 40 40 40 37 38 39 39 39 40 38 40 38 39 - 39 40 40 40 40 40 40 39 40 40 39 39 40 40 38 37 37 40 39 39 39 38 40 34 39 - 38 39 38 39 38 38 39 36 35 39 39 38 35 37 38 38 38 38 38 38 35 33 31 33 34 - 34 33 35 32 33 36 31 36 31 33 33 33 33 36 36 33 37 31 30 32 29 30 28 28 33 - 31 14 28 30 31 30 29 26 31 33 33 31] ->L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 38 39 40 40 40 40 35 40 40 40 40 40 - 38 40 40 40 37 40 40 40 38 40 40 40 40 40 40 40 40 34 38 38 40 40 37 33 35 - 38 38 31 40 37 37 40 37 39 38 39 40 39 35 33 36 34 36 39 38 39 38 37 37 35 - 23 37 34 38 34 38 38 38 38 37 38 36 33 35 36 36 36 33 36 36 38 36 38 33 33 - 29 33 33 31 33 33 34 34 32 28 26 30 30 28 27 30 26 19 29 32 31 28 30 25 29 - 31 30 30 25 31 31 7 31] ->L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 35 40 40 40 40 40 - 39 38 40 40 40 40 40 40 40 40 38 40 40 40 37 40 39 40 40 39 40 40 40 38 40 - 39 37 40 40 39 40 40 39 40 38 39 38 38 39 36 39 27 39 38 39 38 36 37 33 34 - 29 36 33 36 36 36 37 34 38 36 38 35 33 36 36 34 36 33 33 34 34 27 31 33 35 - 33 33 36 35 33 35 33 35 31 33 34 33 34 35 35 35 32 35 33 31 28 28 31 30 31 - 33 28 31 31 33 18 33 28 31 31] ->L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[39 39 39 39 39 39 39 39 39 39 35 39 37 39 39 39 39 39 39 39 39 39 39 39 39 - 38 39 39 39 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 38 - 38 38 29 39 39 39 39 39 36 35 38 35 35 38 37 38 38 38 35 38 38 33 38 36 36 - 38 33 38 38 23 38 31 35 36 38 38 36 37 36 37 36 27 38 38 36 36 37 36 38 38 - 39 38 18 35 38 33 33 33 35 35 38 35 31 38 37 35 36 36 35 33 35 33 33 29 33 - 33 33 35 33 33 35 31 29 30 25 26 30 30 28 30 33] ->L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[39 39 39 38 38 38 38 38 38 38 38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 - 39 39 39 38 38 16 35 27 30 32 26 28 26 38 37 38 33 38 38 27 38 35 38 35 35 - 38 38 33 32 32 35 38 31 39 39 33 39 37 38 39 36 38 36 27 30 28 23 23 35 35 - 35 32 35 31 31 33 33 17 25 26 30 31 30 33 29 29 35 26 32 24 32 23 34] ->L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[39 39 39 39 31 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 38 39 - 39 39 39 39 39 39 39 39 39 39 39 38 36 39 39 39 37 39 36 38 36 35 38 35 35 - 35 38 38 37 37 37 35 35 32 33 36 36 36 35 35 34 37 38 36 36 35 33 34 32 36 - 33 36 36 36 36 36 31 36 33 33 36 33 33 36 36 36 36 23 33 31 31 25 27 23 28 - 28 27 28 33 32 32 29 32 31 25 31 23 31] ->L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 - 39 40 40 40 40 40 40 40 40 40 40 40 39 40 39 37 40 40 39 40 40 39 40 39 39 - 39 40 40 40 39 39 40 39 39 40 40 40 39 40 38 40 39 40 37 39 40 37 39 36 35 - 33 40 35 37 37 36 39 39 38 38 39 39 36 37 36 33 40 36 37 37 34 36 36 35 29 - 36 35 36 33 36 36 35 33 36 36 39 35 33 36 37 36 33 35 38 36 36 35 35 33 37 - 36 35 36 33 34 33 33 33 26 34 33 33 33] ->L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 - 39 40 40 40 37 40 40 40 39 40 39 40 40 39 39 40 39 39 40 40 40 40 40 39 37 - 40 40 38 40 37 35 32 38 40 40 39 37 39 39 36 39 31 40 39 40 40 38 40 39 38 - 38 40 36 39 38 39 36 39 38 39 37 39 38 36 36 36 40 39 39 36 36 36 31 36 36 - 36 36 40 36 37 39 33 36 35 37 38 35 37 38 38 36 36 35 36 36 33 33 16 23 32 - 31 31 31 33 31 23 23 31] ->L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 39 40 40 40 40 40 40 40 40 40 40 40 39 40 38 40 40 38 40 40 40 40 38 40 40 - 40 38 40 40 40 39 40 40 40 40 40 39 39 39 40 40 40 39 40 40 40 39 40 39 39 - 40 27 39 37 40 37 39 36 40 39 36 39 38 38 39 34 33 39 33 38 27 35 35 35 32 - 30 31 32 35 31 23 28 33 32 32 30 27 21 30 32 32 30 30 16 27 27 24 26 17 20 - 19 21 27 30 10 25 31] ->L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 25 35 38 38 38 38 37 38 40 40 40 40 39 40 40 40 40 39 - 37 40 40 40 35 40 40 40 40 40 39 40 40 40 39 35 40 40 40 34 40 40 40 36 39 - 40 39 40 40 40 39 40 40 40 39 40 40 39 37 36 36 29 40 40 39 39 38 40 38 36 - 29 37 36 36 36 37 39 36 36 38 38 38 38 34 39 36 39 38 38 36 38 35 36 36 38 - 38 31 38 35 35 36 35 38 36 33 36 38 35 33 36 27 36 33 36 33 33 33 34 31 31 - 31] ->L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 39 40 40 35 40 40 40 40 40 40 36 40 40 40 40 39 - 37 39 40 40 40 39 40 40 40 40 39 37 40 40 40 39 40 40 40 40 40 40 40 36 39 - 38 40 35 40 40 35 40 38 40 37 37 33 40 40 39 36 35 40 36 36 36 37 38 36 39 - 36 40 33 37 34 37 35 36 38 39 38 38 38 39 38 39 38 36 29 36 36 35 36 33 36 - 33 33 35 35 34 34 35 35 34 29 35 34 34 31 34 30 34 33 34 36 31 33 33 33 33 - 29 24 31 27 29 30 26 31 10 26 27 30] ->L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 - 39 40 40 40 39 40 40 40 40 40 39 40 40 40 40 40 39 39 39 39 40 40 40 39 39 - 40 40 38 40 39 40 40 40 39 39 40 40 40 39 34 40 39 40 37 39 40 37 37 36 36 - 31 39 35 39 38 39 37 38 38 38 38 38 36 38 36 36 39 36 38 39 36 34 38 36 36 - 38 38 39 33 33 35 35 33 33 23 33 31 33 31 31 32] ->L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 17 38 38 - 35 38 38 40 39 40 40 40 40 39 40 40 40 40 39 40 39 40 40 36 40 40 40 40 37 - 40 40 40 40 40 39 40 40 40 40 40 40 38 40 37 37 35 40 40 40 40 39 40 36 36 - 35 40 36 40 36 37 39 39 33 36 40 34 36 33 35 34 36 33 36 34 35 36 36 36 39 - 36 33 37 33 34 36 33 35 33 28 33 33 35 30 32 32 30] ->L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 39 40 40 40 38 38 38 38 38 40 39 40 40 40 40 39 33 40 40 40 39 - 40 40 40 40 40 40 40 40 40 38 40 40 39 39 39 39 40 40 40 38 40 40 35 40 39 - 40 40 40 40 39 39 40 37 36 40 36 36 36 37 35 34 37 37 38 36 38 35 39 38 38 - 36 38 38 38 38 39 38 36 36 35 38 38 36 36 35 35 38 37 23 36 33 33 34 35 36 - 36 35 33 33 36 31 31 31 33 34 33 34 33 33 31 36 33 33 33 33 31 28 28 19 31 - 30 30 18 28 28 28 24 24 31 16 33 31] ->L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 40 40 40 40 38 40 40 40 38 40 40 40 40 40 40 40 40 40 40 39 - 35 40 40 40 40 40 40 40 40 40 39 40 40 38 38 36 38 38 40 39 40 40 40 40 40 - 39 40 36 40 40 40 36 40 40 40 39 38 40 40 39 40 40 38 40 38 40 36 40 39 39 - 40 39 39 39 38 36 38 36 34 27 36 39 34 38 38 39 36 37 36 27 36 33 31 33 33 - 36 29 34 34 36 33 29 33 26 14 28 30 28 33 34 33 34 33 29 33 33 29 31 31 33 - 27 31 30 27 23 20 9 24 17 23 20 22 23 28 31 33] ->L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 37 39 39 39 39 39 - 37 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 36 39 39 39 36 36 - 38 35 38 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 35 38 - 38 39 38 39 39 39 39 37 36 39 38 38 33 39 33 36 39 36 38 39 38 39 38 38 39 - 38 35 39 32 36 39 36 39 33 33 35 33 33 33 33 34 29 33 36 36 36 33 33 32 36 - 32 32 36 36 33 36 32 33 29 27 31 33] ->L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[39 39 39 39 39 39 39 39 36 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 - 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 35 39 39 39 39 36 37 39 39 39 39 39 31 39 39 39 39 39 36 39 39 36 38 36 36 - 36 39 35 39 36 37 39 36 38 39 36 31 36 39 38 38 39 38 34 31 33 23 35 36 36 - 36 34 36 33 36 36 35 23 26 30 28 30 30 35 33 34 35 33 34 34 34 33 35 33 33 - 27 33 33 33 34 36 23 36 18 28 18 28 30] ->L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 37 39 39 39 39 38 38 - 39 37 39 39 39 39 39 39 39 39 39 39 39 39 38 39 38 39 36 39 39 36 39 35 29 - 34 36 34 38 38 39 39 31 38 38 34 37 38 38 36 38 39 38 33 31 33 33 33 36 36 - 36 36 39 31 33 33 34 34 33 31 33 29 34 36 27 34 31 33 33 36 33 33 32 35 36 - 32 32 29 28 32 31 31 28 25 28 28 30] ->L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -[36 36 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 38 35 38 38 38 38 38 38 38 38 38 38 37 38 37 38 38 38 38 35 38 37 38 38 37 - 29 36 38 36 38 36 37 36 35 23 35 38 35 36 36 36 33 38 35 38 33 35 37 35 33 - 35 33 35 36 36 27 33 36 35 31 31 32 31 23 33 26 28 24 29 23 29 10 29 26 26 - 19 24 26 30] ->L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 39 40 35 40 40 40 40 40 35 40 40 40 40 40 40 40 40 40 40 38 - 35 40 40 40 36 40 40 40 40 40 40 40 40 37 37 37 40 40 40 39 40 40 40 39 36 - 40 40 40 40 38 37 40 40 40 38 40 40 39 40 36 39 36 40 37 35 40 37 40 38 38 - 38 39 36 40 36 35 39 34 38 39 38 39 39 35 36 36 40 36 36 36 36 36 36 38 38 - 38 29 38 35 36 35 33 33 29 32 35 32 35 31 32 26 29 19 27 28 27 24 21 22 25 - 27 27 28 25 30] ->L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[39 39 39 39 39 39 39 39 39 39 39 39 39 39 35 39 39 39 39 39 39 39 39 39 39 - 39 38 39 39 39 39 39 39 39 39 36 39 39 38 38 33 38 38 38 39 39 39 39 39 39 - 39 39 39 39 38 39 39 39 39 39 39 38 36 38 37 37 38 37 37 38 37 35 37 36 37 - 39 37 35 39 36 36 39 33 34 17 33 36 34 23 28 30 30 32 33 33 33 31 35 31 31 - 29 33 33 33 33 31 20 31 22 22 28 28 16 34 34 31 33 33] ->L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[38 38 38 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 38 40 40 40 40 40 - 35 40 40 40 38 40 40 40 40 40 40 40 39 40 40 40 38 40 40 38 40 40 39 36 38 - 38 38 34 40 40 37 40 40 40 39 40 40 40 40 39 38 39 40 39 39 40 36 39 39 36 - 35 35 39 37 33 40 35 37 37 39 39 37 36 38 38 38 39 38 29 36 36 33 36 33 34 - 36 33 34 31 33 33 33 33 28 21 24 28 11 25 16 23 21 25 36 33 36 33 34 26 26 - 31 25 31] ->L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 39 40 40 40 40 40 40 39 - 39 40 40 40 40 40 40 40 40 40 39 40 40 39 39 40 40 40 40 39 40 40 40 39 38 - 39 39 40 40 40 39 39 40 39 40 40 40 39 40 36 36 36 40 39 40 40 38 40 37 34 - 35 40 33 39 39 40 31 39 39 38 36 35 35 29 33 31 35 33 36 33 33 33 36 36 36 - 33 36 37 34 36 38 36 36 33 27 35 33 33 36 36 33 36 33 31 33 33 27 34 28 33 - 22 31 33 31 34 33 31] ->L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 - 35 40 40 40 40 40 40 40 39 40 39 40 40 38 40 40 38 40 40 39 40 40 40 40 40 - 40 39 39 40 33 40 40 40 40 40 40 40 38 40 36 40 39 38 40 40 40 36 39 40 40 - 31 40 38 40 39 37 35 39 40 38 35 40 39 39 37 36 36 40 38 33 33 27 35 35 37 - 37 37 37 36 37 35 36 33 33 35 38 38 33 37 34 36 36 34 35 35 35 31 35 31 37 - 33 33 35 35 26 33 33 32 32 31 35 24 35 25 25 30 18 28 27 21 20 23 20 20 26 - 25 27] ->L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[39 39 39 38 36 38 38 38 37 38 33 38 38 39 39 39 39 39 39 37 34 39 39 39 39 - 39 39 39 39 39 39 39 39 39 39 35 39 36 37 37 37 38 31 36 36 36 37 27 39 37 - 39 39 39 39 35 39 37 23 35 39 33 39 39 39 32 38 39 38 37 37 35 32 35 35 35 - 37 34 33 36 36 35 35 32 34 36 33 36 33 36 33 22 29 36 29 29 8 29 31 36 35 - 34 34 31 33 27 29 25 20 25 21 25 29 21 23 29 15 32 26 24 29 26 25 31 30 27 - 29 30 30 31 33] ->L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -[38 38 38 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 39 38 39 39 39 39 39 - 38 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 39 38 39 39 39 39 39 39 39 - 39 39 39 39 38 33 39 39 39 39 39 33 39 39 39 39 39 39 39 37 39 38 39 33 39 - 39 37 38 36 38 36 36 33 36 39 39 39 35 35 35 33 31 38 33 28 30 28 30 36 36 - 36 29 36 33 35 18 28 31 32 32 33 35 35 35 29 33 33 34 32 27 32 28 32] ->L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -[40 40 40 40 40 39 40 40 37 40 40 40 38 38 39 40 40 39 40 35 40 39 40 40 38 - 33 40 40 40 36 40 40 40 39 39 36 40 40 39 36 38 39 39 39 36 39 38 40 35 33 - 36 38 33 40 37 36 37 37 36 36 39 36 33 39 36 37 34 39 38 38 39 36 39 36 31 - 33 39 33 37 35 37 36 36 38 36 35 35 36 33 33 33 39 36 31 35 33 33 35 33 36 - 33 33 36 35 36 36 35 33 36 29 32 36 34 35 34 35 33 31 33 33 31 25 34 31 33 - 33 31 33 28 15 28 15 29 30 28 29 31] ->L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 39 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 - 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 39 40 37 40 40 40 40 40 39 39 - 40 37 40 40 40 39 38 39 39 40 39 40 40 40 39 39 40 40 37 37 40 34 40 33 23 - 33 35 38 35 31 35 33 32 34 37 36 35 38 34 36 36 33 37 37 37 34 33 33 33 36 - 33 36 33 32 32 26 23 30 29 30 32 32 32 33 33 34 34 31 33 33 29 29 34 31 35 - 33 31 33 31 22 31 31 31 23 18 26 23 31 23 24 27 22 10 28 23 28 7 28 20 30 - 24 30] ->L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[27 31 31 38 38 38 38 38 38 38 33 38 38 38 38 38 38 38 38 38 38 38 38 38 38 - 38 38 38 38 38 38 35 36 38 38 38 38 38 38 38 38 38 38 38 33 38 38 38 38 38 - 38 35 38 38 38 33 27 33 38 38 38 38 38 38 38 38 38 38 29 27 38 38 38 35 38 - 35 38 38 38 36 38 35 38 38 38 38 38 36 38 37 36 27 35 38 35 35 35 35 38 38 - 38 33 38 35 35 38 35 25 33 33 35 35 35 30 30 25 27 30 38 38 35 33 33 26 33] ->L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 38 40 40 40 40 38 39 40 40 38 38 40 40 40 40 40 39 40 40 40 40 39 - 40 39 40 40 40 39 40 40 40 40 36 40 36 40 37 37 38 38 38 40 37 40 39 40 40 - 39 37 40 39 40 39 35 37 40 40 40 38 36 39 36 39 39 40 39 40 38 38 37 36 38 - 38 27 39 36 38 33 35 29 31 29 32 35 32 36 36 36 31 35 30 32 28 27 28 31 33 - 31 31 31 33 31 33 29 33 33 33 33 34 32 31 31 8 29 31 27 19 29 16 29 31 31 - 29 26 29 32 19 25 31 32] ->L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 - 35 40 40 40 40 40 40 40 40 40 39 40 40 39 40 40 40 40 40 39 40 40 40 39 35 - 40 37 40 40 40 40 40 40 40 40 40 40 39 40 38 40 38 40 39 39 40 36 40 37 37 - 36 40 36 40 39 40 37 40 38 38 39 38 39 35 39 39 39 38 39 38 33 27 36 36 35 - 39 36 37 38 38 39 33 38 36 35 39 39 39 33 34 33 36 33 36 36 35 31 33 23 31 - 31 31 32 31 33 31 31 31 23 30 18 30] ->L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -[40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 39 - 38 40 40 40 39 40 40 40 40 40 40 40 40 40 39 39 40 40 40 40 40 38 37 40 40 - 40 40 40 40 40 40 40 40 40 40 40 39 38 40 37 37 38 40 37 40 40 37 40 38 39 - 33 40 39 38 40 39 40 38 39 38 38 36 35 35 33 35 33 37 35 29 33 31 31 33 33 - 33 28 33 33 35 33 30 31 33 25 33 33 33 36 36 36 34 33 30 30 32 19 31 19 30 - 28 28 30] ->L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -[28 33 31 38 35 38 38 38 36 38 36 38 38 38 38 38 38 38 38 36 38 38 38 38 38 - 35 38 38 38 38 36 36 36 38 38 38 33 38 38 38 38 38 38 38 35 38 38 38 38 38 - 38 38 31 36 36 33 33 36 30 30 30 35 33 38 38 38 36 38 33 38 38 35 38 38 38 - 38 33 34 30 15 30 27 33 37 38 37 29 33 35 38 35 35 38 34 33 35 35 35 35 35 - 35 35 35 36 38 38 37 35 35 35 32 38 10 7 17 25 18 24 35 33 32 32 32] ->L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -[40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 37 - 37 40 40 40 37 40 40 40 40 40 39 40 40 40 39 36 40 40 40 39 40 40 40 39 39 - 40 37 40 40 40 40 39 40 40 40 39 39 39 40 38 39 39 37 39 40 39 36 40 35 33 - 33 37 38 39 37 39 37 36 39 36 40 40 36 33 35 33 36 36 35 34 35 36 37 28 35 - 33 35 35 34 33 35 28 35 31 33 35 36 33 35 37 34 34 29 33 31 33 19 33 28 31 - 28 29 31 28 33 33 29 23 30] diff -r 10214bba6195 -r 8218f848c2b9 test-data/split_libraries_fastq/sequences.fasta --- a/test-data/split_libraries_fastq/sequences.fasta Mon Jul 10 16:42:21 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,172 +0,0 @@ ->L1S140_0 HWI-EAS440_0386:1:25:1851:1463#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGACATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATA ->L1S105_1 HWI-EAS440_0386:1:25:4646:1592#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG ->L1S281_2 HWI-EAS440_0386:1:25:4380:1628#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTA ->L1S208_3 HWI-EAS440_0386:1:25:1510:1668#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAG ->L1S8_4 HWI-EAS440_0386:1:25:3929:1715#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ->L1S257_5 HWI-EAS440_0386:1:25:2639:1894#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ->L1S8_6 HWI-EAS440_0386:1:25:6139:2018#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGT ->L1S208_7 HWI-EAS440_0386:1:25:3109:2059#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ->L1S281_8 HWI-EAS440_0386:1:25:7669:2080#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCGACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAG ->L1S140_9 HWI-EAS440_0386:1:25:3922:2110#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTT ->L1S105_10 HWI-EAS440_0386:1:25:3806:2202#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAG ->L1S76_11 HWI-EAS440_0386:1:25:6540:2228#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGGTCATCTA ->L1S281_12 HWI-EAS440_0386:1:25:2668:2254#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTT ->L1S76_13 HWI-EAS440_0386:1:25:5054:2329#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ->L1S208_14 HWI-EAS440_0386:1:25:8482:2380#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAG ->L1S208_15 HWI-EAS440_0386:1:25:8261:2388#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ->L1S105_16 HWI-EAS440_0386:1:25:9735:2401#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAG ->L1S8_17 HWI-EAS440_0386:1:25:8948:2437#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA ->L1S140_18 HWI-EAS440_0386:1:25:12681:2438#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAGTACAGTAGAGGTGGG ->L1S281_19 HWI-EAS440_0386:1:25:12916:2459#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ->L1S8_20 HWI-EAS440_0386:1:25:7336:2484#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGG ->L1S76_21 HWI-EAS440_0386:1:25:9578:2531#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ->L1S257_22 HWI-EAS440_0386:1:25:9159:2552#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ->L1S8_23 HWI-EAS440_0386:1:25:11396:2559#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTG ->L1S281_24 HWI-EAS440_0386:1:25:14068:2621#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG ->L1S76_25 HWI-EAS440_0386:1:25:8159:2639#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTA ->L1S140_26 HWI-EAS440_0386:1:25:11175:2690#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGC ->L1S257_27 HWI-EAS440_0386:1:25:8576:2709#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAG ->L1S8_28 HWI-EAS440_0386:1:25:1850:2741#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -AACGTAGGTCCCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGCAGGCG ->L1S105_29 HWI-EAS440_0386:1:25:10428:2744#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ->L1S57_30 HWI-EAS440_0386:1:25:12398:2774#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ->L1S76_31 HWI-EAS440_0386:1:25:14110:2805#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTA ->L1S140_32 HWI-EAS440_0386:1:25:14127:2819#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTT ->L1S57_33 HWI-EAS440_0386:1:25:1968:2841#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTGGTGAAAGATCGAAGCTTAACTTCGGTAAGCCATGGAAACCGTAGGGCT ->L1S105_34 HWI-EAS440_0386:1:25:11767:2838#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAG ->L1S105_35 HWI-EAS440_0386:1:25:4402:2852#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCGACCGTAAAATTGCAGTTGATACTGGCTG ->L1S257_36 HWI-EAS440_0386:1:25:11319:2883#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGACTAG ->L1S281_37 HWI-EAS440_0386:1:25:6586:2930#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTC ->L1S8_38 HWI-EAS440_0386:1:25:8471:2931#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATAACTTGAGTGCAG ->L1S76_39 HWI-EAS440_0386:1:25:14208:2940#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGTCAGTCTTGAGTACAGTAGAGGTG ->L1S76_40 HWI-EAS440_0386:1:25:6132:2959#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAAGTGAAAGCCCAGGGCTCAACCCCGGGACTGCTTTTGAAACTGCAGGGCTAGAGTGCAGGAGGGGCAAGTGG ->L1S57_41 HWI-EAS440_0386:1:25:2692:2971#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGGGCGG ->L1S57_42 HWI-EAS440_0386:1:25:1831:2983#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGC ->L1S281_43 HWI-EAS440_0386:1:25:6335:2989#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGCTGAAACTGGCAGTCTTGAGTACAG ->L1S140_44 HWI-EAS440_0386:1:25:10162:3028#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGGTGCAAGCGATAATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGGGTGAAATCCCACAGCCCAACTGTGGAACTGCCTTTGATACTGCCAGGCTTGAGTACCG ->L1S140_45 HWI-EAS440_0386:1:25:4348:3042#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ->L1S208_46 HWI-EAS440_0386:1:25:5972:3048#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGAACTGCATTGGAAACTGTTTGA ->L1S140_47 HWI-EAS440_0386:1:25:9861:3050#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCT ->L1S208_48 HWI-EAS440_0386:1:25:10090:3050#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ->L1S8_49 HWI-EAS440_0386:1:25:3139:3077#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACTGGGGGCTCAACCTCCAGCCTGCATTTGAAACTGTAGTTCTTGAGTGCTG ->L1S105_50 HWI-EAS440_0386:1:25:9110:3081#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGCAGG ->L1S76_51 HWI-EAS440_0386:1:25:17734:3100#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCTGGAAACTGTTAGGCTTGAGTGCAGGAGAGGAAAGGG ->L1S57_52 HWI-EAS440_0386:1:25:8423:3141#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ->L1S8_53 HWI-EAS440_0386:1:25:14009:3149#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAG ->L1S257_54 HWI-EAS440_0386:1:25:3100:3158#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGGTGTCTTGAGTACAGTAGAGGC ->L1S257_55 HWI-EAS440_0386:1:25:9072:3171#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGGCGG ->L1S105_56 HWI-EAS440_0386:1:25:15751:3166#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAG ->L1S208_57 HWI-EAS440_0386:1:25:3473:3225#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAGGTGAAATTTCGGGGCTCAACCCTGAACGTGCCTCTAATACTGTTGAGCTAGAGAGTAGTTGCGGTAGG ->L1S208_58 HWI-EAS440_0386:1:25:4755:3229#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAGG ->L1S281_59 HWI-EAS440_0386:1:25:3032:3237#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCGACCTTGAGTGCAACAGAG ->L1S208_60 HWI-EAS440_0386:1:25:1630:3249#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGCGTGTCGGAGG ->L1S76_61 HWI-EAS440_0386:1:25:3312:3259#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGC ->L1S57_62 HWI-EAS440_0386:1:25:3550:3291#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ->L1S257_63 HWI-EAS440_0386:1:25:5478:3298#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTG ->L1S281_64 HWI-EAS440_0386:1:25:15346:3296#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGT ->L1S76_65 HWI-EAS440_0386:1:25:3468:3322#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGCTGTGCAAGACAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGTGACTGCACGGCTAGAGTTTGTCAGAGGAGG ->L1S281_66 HWI-EAS440_0386:1:25:4199:3321#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTTGAAACTGTGGAGCTGGAGTGCAGGAGAGGCAAGTGG ->L1S140_67 HWI-EAS440_0386:1:25:8568:3330#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ->L1S57_68 HWI-EAS440_0386:1:25:12050:3335#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGG ->L1S140_69 HWI-EAS440_0386:1:25:4994:3354#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTGAGGCAG ->L1S140_70 HWI-EAS440_0386:1:25:12091:3381#0/1 orig_bc=ATGGCAGCTCTA new_bc=ATGGCAGCTCTA bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGG ->L1S105_71 HWI-EAS440_0386:1:25:8594:3395#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTT ->L1S281_72 HWI-EAS440_0386:1:25:2052:3400#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAAGTGAAAGCCCGGGGCTTAACCCCGGGACTGCTTTGGAAACTGTAGGAC ->L1S76_73 HWI-EAS440_0386:1:25:11776:3405#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAG ->L1S76_74 HWI-EAS440_0386:1:25:11052:3431#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGA ->L1S105_75 HWI-EAS440_0386:1:25:9796:3444#0/1 orig_bc=AGTGCGATGCGT new_bc=AGTGCGATGCGT bc_diffs=0 -TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAAGTGAAAATCCGGGGCTCAACCCCGGAACTGCTTTTGAAACTGTGAGGCTAGAGTGCAGGAGGGGTGAGTGGAATTCCTAGTG ->L1S8_76 HWI-EAS440_0386:1:25:2235:3462#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAGTCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA ->L1S57_77 HWI-EAS440_0386:1:25:6708:3509#0/1 orig_bc=ACACACTATGGC new_bc=ACACACTATGGC bc_diffs=0 -TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGATGTGAAAACCCAGGGCTTAACCCTGGGACTGCATTGGAAACTGTCTGGCTCGAG ->L1S281_78 HWI-EAS440_0386:1:25:13919:3507#0/1 orig_bc=CCTCTCGTGATC new_bc=CCTCTCGTGATC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGAAACTGGCAGTCTTGAGTACAGTAGAGGTGG ->L1S208_79 HWI-EAS440_0386:1:25:7788:3570#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAAGTGAAATGCGGGGGCTCAACCCCTGAACTGCTTTGGAAACTGTCATGCTAGAGTGCAGGAGGGGTGAGCGGAATTCCTAGTG ->L1S257_80 HWI-EAS440_0386:1:25:14850:3657#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCGGTGAAAGTTTGTGGCTCAACCATAAAATTGCCGTTGAAACTGGGTTACTTGAGTG ->L1S76_81 HWI-EAS440_0386:1:25:11581:3662#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGTACTAGAGTGTCGGAGGG ->L1S76_82 HWI-EAS440_0386:1:25:16965:3660#0/1 orig_bc=ACTACGTGTGGT new_bc=ACTACGTGTGGT bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAGTTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAG ->L1S208_83 HWI-EAS440_0386:1:25:8197:3691#0/1 orig_bc=CTGAGATACGCG new_bc=CTGAGATACGCG bc_diffs=0 -TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGATGTGAAAGCCCGGGGCTCAACCCCGGTACTGCATTGGAAACTGTCGGACTAGAGTGTCG ->L1S8_84 HWI-EAS440_0386:1:25:11617:3701#0/1 orig_bc=AGCTGACTAGTC new_bc=AGCTGACTAGTC bc_diffs=0 -TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGCTGTCTTGAG ->L1S257_85 HWI-EAS440_0386:1:25:15484:3705#0/1 orig_bc=CCGACTGAGATG new_bc=CCGACTGAGATG bc_diffs=0 -TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCGGTGAAATTTTGGTGCTTAACACCAAACGTGCCGTTGATACTGCTGGGCTAGAGAGTAGTTGCGG