Mercurial > repos > iuc > qualimap_bamqc

annotate qualimap_bamqc.xml @ 4:19ece8afbaab draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
author iuc
date Wed, 20 May 2020 15:56:11 -0400
parents 5f8e69cc4c6e
children 30a201c9c310
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19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
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1 <tool id="qualimap_bamqc" name="QualiMap BamQC" version="@VERSION@+galaxy3">
0
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2 <macros>
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3 <import>qualimap_macros.xml</import>
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4 </macros>
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5 <expand macro="requirements" />
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6 <expand macro="version_command" />
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7 <command detect_errors="exit_code"><![CDATA[
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8 #import os
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9 #import re
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10 @SET_JAVA_OPTS@ &&
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11
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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12 ## Set some default file names and paths
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13 ## where we expect tool output to end up.
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14 ## Note that most of these need to be overwritten if the user is
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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15 ## interested in regions *outside* those defined in a custom regions
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16 ## file.
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17 #set $out_dir = 'results'
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18 #set $report_name = 'qualimapReport'
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19 #set $summary_report = 'genome_results.txt'
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20 #set $coverage_file = os.path.join($out_dir, 'coverage.txt')
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21 ## This is the only file path that qualimap does not calculate
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22 ## from $out_dir.
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23 #set $per_base_coverage_target = $coverage_file
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24
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25 #if str($stats_regions.region_select) == 'custom_regions':
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26 ## Have qualimap detect file format by suffix
4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
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27 ## since its autodetection is unreliable.
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28 #set $regions_file = '.'.join(['regions', str($stats_regions.regions.ext)])
4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
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29 ln -s '${stats_regions.regions}' ${regions_file} &&
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30
0
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31 #if $stats_regions.outside_stats:
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32 #set $report_name = 'qualimapReportOutsideRegions'
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33 #set $summary_report = 'outside_results.txt'
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34 #set $coverage_file = os.path.join(
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35 $out_dir, 'outside_coverage.txt'
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36 )
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37 #if $per_base_coverage:
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38 #set $per_base_coverage_target = '/dev/null'
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39 #end if
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40 #end if
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41 #end if
1
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42
4
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43 #set input_name = re.sub('[^\w]', '_', str($input1.element_identifier))
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44 ln -s '$input1' '$input_name' &&
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45
1
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46 qualimap bamqc
3
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47 -bam '$input_name' -outdir results -outformat html
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48 --collect-overlap-pairs
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49 #if str($stats_regions.region_select) == 'custom_regions':
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50 -gff ${regions_file} ${stats_regions.outside_stats}
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51 #end if
0
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52 #if $per_base_coverage:
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53 $per_base_coverage $per_base_coverage_target
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54 #end if
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55 -nw ${plot_specific.n_bins}
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56 ${plot_specific.paint_chromosome_limits}
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57 #if $plot_specific.genome_gc_distr:
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58 --genome-gc-distr ${plot_specific.genome_gc_distr}
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59 #end if
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60 -hm ${plot_specific.homopolymer_size}
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61
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62 #if $duplicate_skipping:
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63 --skip-duplicated
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64 #if str($duplicate_skipping) == '0,1':
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65 --skip-dup-mode 2
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66 #else:
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67 --skip-dup-mode ${duplicate_skipping}
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68 #end if
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69 #end if
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70 -nt \${GALAXY_SLOTS:-1} &&
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71
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72 #if $per_base_coverage:
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73 mv $coverage_file '$output_per_base_coverage' &&
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74 #end if
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75 @MASSAGE_OUTPUT@
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76 ]]></command>
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77 <inputs>
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78 <param argument="-bam" name="input1" type="data" format="bam"
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79 label="Mapped reads input dataset" />
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80 <conditional name="stats_regions">
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81 <param name="region_select" type="select" label="Reference genome regions to calculate mapping statistics for">
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82 <option value="all">All (whole genome)</option>
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83 <option value="custom_regions">Select regions</option>
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84 </param>
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85 <when value="all" />
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86 <when value="custom_regions">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
87 <param argument="-gff" name="regions" type="data" format="gff,gtf,bed"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
88 label="Dataset specifying regions" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
89 <param argument="-os" name="outside_stats" type="boolean" truevalue="--outside-stats" falsevalue="" checked="false"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
90 label="Invert regions"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
91 help="If selected, report read statistics *outside* the regions in the regions file." />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
92 </when>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
93 </conditional>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
94 <param argument="-oc" name="per_base_coverage" type="boolean" truevalue="--output-genome-coverage" falsevalue="" checked="false"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
95 label="Generate per-base coverage output"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
96 help="Produce additional tabular output listing the coverage at every site (omitting only zero-coverage positions) in the selected regions of the genome. Caution: Will generate a huge dataset for anything but small input genomes or restricted regions!" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
97 <param argument="--skip-dup-mode" name="duplicate_skipping" type="select" display="checkboxes" multiple="true" optional="true"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
98 label="Skip duplicate reads">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
99 <option value="0" selected="true">Reads flagged as duplicates in input</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
100 <option value="1">Duplicates detected by Qualimap</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
101 </param>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
102 <section name="plot_specific" title="Settings affecting specific plots" expanded="false">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
103 <param argument="-nw" name="n_bins" type="integer" value="400"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
104 label="Number of bins to use in across-reference plots"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
105 help="Affected plots: Coverage, Mapping Quality and Insert Size across reference, Mapped reads GC-content distribution; the value determines the resolution of the affected plots. Note: The lower the value, the higher the memory usage of the tool!" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
106 <param argument="-c" name="paint_chromosome_limits" type="boolean" truevalue="--paint-chromosome-limits" falsevalue="" checked="true"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
107 label="Draw chromosome limits"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
108 help="Affected plots: Coverage, Mapping Quality and Insert Size across reference; in across-reference plots, indicate chromosome boundaries with dotted lines and labels" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
109 <param argument="-gd" name="genome_gc_distr" type="select" optional="true"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
110 label="Plot expected GC-content distribution of the following reference genome"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
111 help="Affected plot: Mapped reads GC-content distribution; include a precalculated GC-content distribution for the selected (Qualimap-supported) reference genome in the plot">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
112 <option value="hg19">Human genome (hg19)</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
113 <option value="mm9">Mouse genome (mm9)</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
114 <option value="mm10">Mouse genome (mm10)</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
115 </param>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
116 <param argument="-hm" name="homopolymer_size" type="integer" value="3" min="2"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
117 label="Homopolymer size"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
118 help="Affected plot: Homopolymer indels; sets the minimal number of consecutive bases that define a homopolymer" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
119 </section>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
120 </inputs>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
121 <outputs>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
122 <data name="output_html" format="html"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
123 label="${tool.name} report on ${on_string}" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
124 <data name="output_per_base_coverage" format="tsv"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
125 label="${tool.name} per-base coverage on ${on_string}">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
126 <filter>per_base_coverage</filter>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
127 </data>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
128 <collection name="raw_data" type="list"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
129 label="Raw data for ${tool.name} on ${on_string}">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
130 <data name="genome_results" format="txt" from_work_dir="results/summary_report.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
131 <data name="coverage_across_reference" format="tsv" from_work_dir="results/coverage_across_reference.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
132 <data name="coverage_histogram" format="tsv" from_work_dir="results/coverage_histogram.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
133 <data name="genome_fraction_coverage" format="tsv" from_work_dir="results/genome_fraction_coverage.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
134 <data name="duplication_rate_histogram" format="tsv" from_work_dir="results/duplication_rate_histogram.txt" />
4
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
135 <data name="homopolymer_indels" format="tsv" from_work_dir="results/homopolymer_indels.txt" />
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
136 <data name="insert_size_across_reference" format="tsv" from_work_dir="results/insert_size_across_reference.txt" />
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
137 <data name="insert_size_histogram" format="tsv" from_work_dir="results/insert_size_histogram.txt" />
0
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
138 <data name="mapped_reads_clipping_profile" format="tsv" from_work_dir="results/mapped_reads_clipping_profile.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
139 <data name="mapped_reads_gc-content_distribution" format="tsv" from_work_dir="results/mapped_reads_gc-content_distribution.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
140 <data name="mapped_reads_nucleotide_content" format="tsv" from_work_dir="results/mapped_reads_nucleotide_content.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
141 <data name="mapping_quality_across_reference" format="tsv" from_work_dir="results/mapping_quality_across_reference.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
142 <data name="mapping_quality_histogram" format="tsv" from_work_dir="results/mapping_quality_histogram.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
143 </collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
144 </outputs>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
145 <tests>
4
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
146 <test expect_num_outputs="15">
0
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
147 <param name="input1" value="test_mapped_reads.bam"/>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
148 <output name="output_html" ftype="html">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
149 <assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
150 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
151 </assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
152 </output>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
153 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
154 <element name="genome_results" file="genome_results_default.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
155 </output_collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
156 </test>
4
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
157 <test expect_num_outputs="16">
0
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
158 <param name="input1" value="test_mapped_reads.bam" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
159 <param name="per_base_coverage" value="true" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
160 <output name="output_html" ftype="html">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
161 <assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
162 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
163 </assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
164 </output>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
165 <output name="output_per_base_coverage" file="per_base_coverage_default.txt" ftype="tsv" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
166 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
167 <element name="genome_results" file="genome_results_default.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
168 </output_collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
169 </test>
4
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
170 <test expect_num_outputs="15">
0
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
171 <param name="input1" value="test_mapped_reads.bam"/>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
172 <conditional name="stats_regions">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
173 <param name="region_select" value="custom_regions" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
174 <param name="regions" value="features.gtf" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
175 </conditional>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
176 <output name="output_html" ftype="html">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
177 <assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
178 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
179 </assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
180 </output>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
181 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
182 <element name="genome_results" file="genome_results_inside_features.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
183 </output_collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
184 </test>
4
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
185 <test expect_num_outputs="16">
0
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
186 <param name="input1" value="test_mapped_reads.bam" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
187 <conditional name="stats_regions">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
188 <param name="region_select" value="custom_regions" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
189 <param name="regions" value="features.gtf" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
190 </conditional>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
191 <param name="per_base_coverage" value="true" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
192 <output name="output_html" ftype="html">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
193 <assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
194 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
195 </assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
196 </output>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
197 <output name="output_per_base_coverage" file="per_base_coverage_inside_features.txt" ftype="tsv" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
198 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
199 <element name="genome_results" file="genome_results_inside_features.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
200 </output_collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
201 </test>
4
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
202 <test expect_num_outputs="16">
0
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
203 <param name="input1" value="test_mapped_reads.bam" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
204 <conditional name="stats_regions">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
205 <param name="region_select" value="custom_regions" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
206 <param name="regions" value="features.gtf" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
207 <param name="outside_stats" value="true" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
208 </conditional>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
209 <param name="per_base_coverage" value="true" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
210 <output name="output_html" ftype="html">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
211 <assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
212 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
213 </assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
214 </output>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
215 <output name="output_per_base_coverage" file="per_base_coverage_outside_features.txt" ftype="tsv" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
216 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
217 <element name="genome_results" file="genome_results_outside_features.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
218 </output_collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
219 </test>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
220 </tests>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
221 <help><![CDATA[
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
222 **What it does**
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
223
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
224 **Qualimap BAM QC** lets you evaluate the quality of aligned reads data in BAM
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
225 format. The tool summarizes basic statistics of the alignment (number of reads,
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
226 coverage, GC-content, etc.) and produces a number of useful graphs for their
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
227 interpretation.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
228
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
229 The analysis can be performed with any kind of sequencing data, such as
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
230 whole-genome sequencing, exome sequencing, RNA-seq or ChIP-seq data.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
231
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
232 In addition, it is possible to provide an annotation file so the results are
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
233 computed for the reads mapping inside (and optionally outside) of the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
234 corresponding genomic regions, which can be especially useful for evaluating
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
235 target-enrichment sequencing studies.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
236
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
237 Input
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
238 =====
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
239
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
240 *Mapped reads input dataset*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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diff changeset
241
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
242 The dataset holding the mapped reads to carry out the analysis with.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
243
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
244 *Dataset specifying regions*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
245
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
246 If you decide to calculate mapping statistics for selected regions of the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
247 reference genome (instead of for the whole genome), you need to specify the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
248 regions through this additional dataset in gtf, gff or bed format.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
249
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
250 .. class:: infomark
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
251
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
252 A typical problem when working with regions (and genome annotation data, in general) is potential inconsistency between the chromosome names used in the mapped reads input versus those used to define the regions. In the case of the human genome, for example, UCSC data has chromosomes starting with a 'chr' prefix, which is lacking from Ensemble data. This simple form of the problem is handled by Qualimap: if chromosome names in the regions input have a 'chr' prefix, Qualimap will add that prefix to the mapped reads chromosome names as needed. For more complex cases you will have to adjust your inputs manually.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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diff changeset
253
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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254
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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255 Parameters
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256 ----------
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257
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258 *Reference genome regions to calculate mapping statistics for*
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259
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260 Choose whether you would like to have mapping statistics reported across
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261
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262 - the entire reference genome
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263 (as specified in the header of the mapped reads input)
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264
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265 - specific regions of the reference
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266
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267 In the second case, you need to select a *Dataset specifying regions* (see
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268 above). Using the *Invert regions* switch you can then indicate whether you
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269 want to select or exclude the regions in this dataset.
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270
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271 *Generate per-base coverage output*
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272
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273 *Skip duplicate reads*
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274
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275 The tool lets you skip alignments of duplicate reads from the analysis.
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276 Depending on whether you select none, either one, or both of the available
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277 options, you can decide to:
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278
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279 - not correct for duplicate reads at all (*e.g.* because you have removed them
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280 at an earlier step with some dedicated tool)
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281 - identify and flag duplicate reads with a dedicated tool (like ``Picard
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282 MarkDuplicates`` or ``samtools markdup``), then have Qualimap ignore the
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283 duplicate-flagged reads (recommended, most flexible option since other tools
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284 can be told to ignore the same reads)
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285 - have Qualimap identify potential duplicates by itself and ignore them
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286 - combine external and Qualimap-internal duplicate detection for extra
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287 stringency
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288
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289 Independent of your selection, the HTML report will always list (in the
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290 `Globals` section of the `Summary`) the number of duplicated reads estimated by
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291 Qualimap. If you choose to skip duplicates, you will also be informed about the
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292 number of skipped reads in that same section and, if you instruct Qualimap to
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293 look for the duplicate flag on reads, the number of reads flagged as duplicates
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294 will also be reported here.
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295
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296 **Section: Settings affecting specific plots**
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297
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298 Parameters in this section only affect some (or even only one) of the plots
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299 contained in the HTML report (and the corresponding part of the *Raw Data*
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300 output collection).
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301
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302 For most of these options, the parameter help above should be descriptive
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303 enough. Just a few more words on two of them:
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304
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305 *Number of bins to use in across-reference plots*
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306
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307 This value is used for computing the various graphs that plot information
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308 across the reference. Basically, the reference genome gets split into the given
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309 number of bins, and reads falling in the same bin are aggregated in the
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310 statistics of that bin.
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311
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312 Thus, the higher the number of bins, the higher the resolution of the plots,
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313 but more bins also require longer time for their statistics to be computed.
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314 Less bins, on the other hand, mean more reads will have to be aggregated per
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315 bin and this comes with higher memory requirements. Hence, if the tool fails
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316 with an ``Out Of Memory`` error, you may want to rerun it with a higher bin
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317 number.
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318
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319 *Plot expected GC-content distribution of the following reference genome*
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320
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321 The choice of reference genomes with pre-calculated GC distributions is built
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322 into Qualimap.
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323
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324 Future releases of Qualimap may include more choices, but the current version
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325 is limited to those offered here.
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326
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327
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328 Outputs
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329 =======
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330
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331 HTML Report
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332 -----------
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333
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334 **Summary Section**
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335
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336 *Globals*
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337
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338 This section contains information about the total number of reads, number of mapped reads, paired-end mapping performance, read length distribution,
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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339 number of clipped reads and duplication rate (estimated from the start positions of read alignments).
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340
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341 *ACGT Content*
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342
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343 Nucleotide content and GC percentage in the mapped reads.
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344
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345 *Coverage*
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346
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347 Mean and standard deviation of the coverage depth.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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348
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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349 *Mapping quality*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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350
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
351 Mean mapping quality of the mapped reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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352
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
353 *Insert size*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
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354
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
355 Mean, standard deviation and percentiles of the insert size distribution if applicable. The features are computed based on the TLEN field of the SAM file.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
356
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
357 *Mismatches and indels*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
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358
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
359 The section reports general alignment error rate (computed as a ratio of total collected edit distance to the number of mapped bases), total number of mismatches and total number of indels (computed from the CIGAR values). Additionally fraction of the homopolymer indels among total indels is provided. Note, the error rate and mismatches metrics are based on optional fields of a SAM record (NM for edit distance, MD for mismatches). The features are not reported if these fields are missing in the SAM file.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
360
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
361 *Chromosome stats*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
362
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
363 Number of mapped bases, mean and standard deviation of the coverage depth for each chromosome as defined by the header of the SAM file.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
364
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
365 For region-based analysis the information is given inside of regions, including some additional information like, for example, number of correct strand reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
366
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
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367
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
368 **Plots**
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
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369
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
370 *Coverage Across Reference*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
371
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
372 This plot consists of two figures.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
373 The upper figure provides the coverage distribution (red line) and coverage
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
374 deviation across the reference sequence.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
375 The lower figure shows GC content across reference (black line) together with
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
376 its average value (red dotted line).
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
377
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
378 *Coverage Histogram*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
379
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
380 Histogram of the number of genomic locations having a given coverage rate.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
381 The bins of the x-axis are conveniently scaled by aggregating some coverage
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
382 values in order to produce a representative histogram also in presence of the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
383 usual NGS peaks of coverage.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
384
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
385 *Coverage Histogram (0-50X)*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
386
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
387 Similar to the previous plot, but in this graph genome locations with a
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
388 coverage greater than 50X are grouped into the last bin.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
389 By doing so a higher resolution of the most common values for the coverage rate
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
390 is obtained.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
391
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
392 *Genome Fraction Coverage*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
393
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
394 Provides a visual way of knowing how much reference has been sequenced to at
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
395 least a given coverage rate.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
396 This graph should be interpreted as in this example:
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
397 If one aims for a coverage rate of at least 25X (x-axis), how much of the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
398 reference (y-axis) will be considered?
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
399
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
400 *Duplication Rate Histogram*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
401
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
402 This plot shows the distribution of duplicated reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
403 Due to several factors (*e.g.* amount of starting material, sample preparation,
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
404 *etc.*) it is possible that the same fragments are sequenced several times.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
405 For some experiments where enrichment is used (*e.g.* ChIP-seq ) this is
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
406 expected to some degree.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
407 For most experiments, however, a high duplication level of the reads indicates
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
408 some unwanted bias.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
409
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
410 *Mapped Reads Nucleotide Content*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
411
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
412 This plot shows the nucleotide content per position of the mapped reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
413
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
414 *Mapped Reads GC Content Distribution*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
415
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
416 This graph shows the distribution of GC-content per mapped read.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
417 If compared with a precomputed genome distribution, this plot allows to check
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
418 if there is a shift in the GC content.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
419
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
420 *Mapped Reads Clipping Profile*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
421
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
422 Represents the percentage of clipped bases across the reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
423 Technically, the clipping is detected via SAM format CIGAR codes ‘H’
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
424 (hard clipping) and ‘S’ (soft clipping).
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
425 In addition, the total number of clipped reads can be found in the report
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
426 `Summary` section.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
427
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
428 This plot is not shown if no clipped reads are found.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
429
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
430 *Homopolymer Indels*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
431
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
432 This bar plot shows the number of indels that are located within A, C, G and T
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
433 homopolymers, respectively, as well as the number of indels that are not within
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
434 any homopolymer. Large numbers of homopolymer indels may indicate a problem in
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
435 the sequencing process.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
436 Technically, Qualimap identifies indels from the CIGAR code of the aligned
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
437 reads. Indel statistics can also be found in a dedicated section of the report
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
438 `Summary`.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
439
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
440 This graph is not shown if the sample doesn’t contain any indels.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
441
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
442 *Mapping Quality Across Reference*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
443
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
444 This plot provides the mapping quality distribution across the reference.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
445 To construct the plot, the mean mapping quality is computed for each bin.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
446
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
447 *Mapping Quality Histogram*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
448
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
449 Histogram of the number of genomic locations having a given mapping quality.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
450 To construct the histogram the mean mapping quality is computed at each genome
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
451 position with non-zero coverage and collected.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
452 According to the SAM/BAM format specifications, the range for the mapping
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
453 quality score is [0-255].
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
454
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
455 *Insert Size Across Reference*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
456
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
457 This plot provides the insert size distribution across the reference.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
458 Technically, the insert size of each pair of aligned reads is collected from
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
459 the SAM alignment field `TLEN`. Only positive values are taken into account.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
460 To construct the plot, the mean insert size is computed for each bin.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
461
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
462 *Insert Size Histogram*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
463
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
464 Histogram of insert size distribution.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
465
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
466
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
467 Raw Data
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
468 --------
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
469
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
470 This is a *Collection* of 10 individual datasets.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
471
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
472 The *genome_results* dataset provides a plain-text summary of key statistics,
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
473 most of which can also be found in the *Summary* section of the *HTML Report*.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
474
4
19ece8afbaab "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit e5a96dae9473c2083a2312d1d00b1e55c27e1620"
iuc
parents: 3
diff changeset
475 The remaining 12 datasets hold the tabular raw data underlying the plots of the corresponding names in the *HTML Report*.
0
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
476
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
477
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
478 Per-base coverage
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
479 -----------------
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
480
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
481 Optional. This is a tabular dataset listing the coverage of every base in the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
482 reference genome unless that coverage is zero. Since its content is
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
483 uncompressed text, this dataset can easily become huge, and it is recommended
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
484 that you generate this dataset only for very small genomes or very limited
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
485 regions of larger genomes.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
486 ]]> </help>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
487 <expand macro="citations"/>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
488 </tool>