Mercurial > repos > iuc > qualimap_bamqc

annotate qualimap_bamqc.xml @ 2:e7fd6754d093 draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 3c0443b29b1fac5ec193912b0c03a79d75ba731a"
author iuc
date Mon, 13 Jan 2020 13:04:33 -0500
parents 4a89c6f84425
children 5f8e69cc4c6e
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4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
iuc
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1 <tool id="qualimap_bamqc" name="QualiMap BamQC" version="@VERSION@+galaxy1">
0
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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2 <macros>
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3 <import>qualimap_macros.xml</import>
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4 </macros>
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5 <expand macro="requirements" />
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6 <expand macro="version_command" />
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7 <command detect_errors="exit_code"><![CDATA[
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8 #import os
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9 @SET_JAVA_OPTS@ &&
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10
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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11 ## Set some default file names and paths
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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12 ## where we expect tool output to end up.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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13 ## Note that most of these need to be overwritten if the user is
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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14 ## interested in regions *outside* those defined in a custom regions
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15 ## file.
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16 #set $out_dir = 'results'
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17 #set $report_name = 'qualimapReport'
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18 #set $summary_report = 'genome_results.txt'
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19 #set $coverage_file = os.path.join($out_dir, 'coverage.txt')
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20 ## This is the only file path that qualimap does not calculate
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21 ## from $out_dir.
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22 #set $per_base_coverage_target = $coverage_file
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23
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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24 #if str($stats_regions.region_select) == 'custom_regions':
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25 ## Have qualimap detect file format by suffix
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26 ## since its autodetection is unreliable.
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27 #set $regions_file = '.'.join(['regions', str($stats_regions.regions.ext)])
4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
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28 ln -s '${stats_regions.regions}' ${regions_file} &&
4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
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29
0
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30 #if $stats_regions.outside_stats:
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31 #set $report_name = 'qualimapReportOutsideRegions'
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32 #set $summary_report = 'outside_results.txt'
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33 #set $coverage_file = os.path.join(
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34 $out_dir, 'outside_coverage.txt'
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35 )
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36 #if $per_base_coverage:
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37 #set $per_base_coverage_target = '/dev/null'
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38 #end if
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39 #end if
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40 #end if
1
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41
4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
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42 qualimap bamqc
4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
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43 -bam '$input1' -outdir results -outformat html
4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
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44 --collect-overlap-pairs
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45 #if str($stats_regions.region_select) == 'custom_regions':
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46 -gff ${regions_file} ${stats_regions.outside_stats}
4a89c6f84425 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit 08beeabf7a8e91d0e95f5cdb71249dd6efe2bd46"
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47 #end if
0
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48 #if $per_base_coverage:
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49 $per_base_coverage $per_base_coverage_target
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50 #end if
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51 -nw ${plot_specific.n_bins}
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52 ${plot_specific.paint_chromosome_limits}
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53 #if $plot_specific.genome_gc_distr:
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54 --genome-gc-distr ${plot_specific.genome_gc_distr}
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55 #end if
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56 -hm ${plot_specific.homopolymer_size}
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57
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58 #if $duplicate_skipping:
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59 --skip-duplicated
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60 #if str($duplicate_skipping) == '0,1':
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61 --skip-dup-mode 2
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62 #else:
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63 --skip-dup-mode ${duplicate_skipping}
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64 #end if
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65 #end if
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66 -nt \${GALAXY_SLOTS:-1} &&
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67
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68 #if $per_base_coverage:
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69 mv $coverage_file '$output_per_base_coverage' &&
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70 #end if
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71 @MASSAGE_OUTPUT@
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72 ]]></command>
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73 <inputs>
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74 <param argument="-bam" name="input1" type="data" format="bam"
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75 label="Mapped reads input dataset" />
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76 <conditional name="stats_regions">
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77 <param name="region_select" type="select" label="Reference genome regions to calculate mapping statistics for">
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78 <option value="all">All (whole genome)</option>
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79 <option value="custom_regions">Select regions</option>
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80 </param>
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81 <when value="all" />
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82 <when value="custom_regions">
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83 <param argument="-gff" name="regions" type="data" format="gff,gtf,bed"
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84 label="Dataset specifying regions" />
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85 <param argument="-os" name="outside_stats" type="boolean" truevalue="--outside-stats" falsevalue="" checked="false"
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86 label="Invert regions"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
87 help="If selected, report read statistics *outside* the regions in the regions file." />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
88 </when>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
89 </conditional>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
90 <param argument="-oc" name="per_base_coverage" type="boolean" truevalue="--output-genome-coverage" falsevalue="" checked="false"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
91 label="Generate per-base coverage output"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
92 help="Produce additional tabular output listing the coverage at every site (omitting only zero-coverage positions) in the selected regions of the genome. Caution: Will generate a huge dataset for anything but small input genomes or restricted regions!" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
93 <param argument="--skip-dup-mode" name="duplicate_skipping" type="select" display="checkboxes" multiple="true" optional="true"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
94 label="Skip duplicate reads">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
95 <option value="0" selected="true">Reads flagged as duplicates in input</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
96 <option value="1">Duplicates detected by Qualimap</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
97 </param>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
98 <section name="plot_specific" title="Settings affecting specific plots" expanded="false">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
99 <param argument="-nw" name="n_bins" type="integer" value="400"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
100 label="Number of bins to use in across-reference plots"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
101 help="Affected plots: Coverage, Mapping Quality and Insert Size across reference, Mapped reads GC-content distribution; the value determines the resolution of the affected plots. Note: The lower the value, the higher the memory usage of the tool!" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
102 <param argument="-c" name="paint_chromosome_limits" type="boolean" truevalue="--paint-chromosome-limits" falsevalue="" checked="true"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
103 label="Draw chromosome limits"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
104 help="Affected plots: Coverage, Mapping Quality and Insert Size across reference; in across-reference plots, indicate chromosome boundaries with dotted lines and labels" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
105 <param argument="-gd" name="genome_gc_distr" type="select" optional="true"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
106 label="Plot expected GC-content distribution of the following reference genome"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
107 help="Affected plot: Mapped reads GC-content distribution; include a precalculated GC-content distribution for the selected (Qualimap-supported) reference genome in the plot">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
108 <option value="hg19">Human genome (hg19)</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
109 <option value="mm9">Mouse genome (mm9)</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
110 <option value="mm10">Mouse genome (mm10)</option>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
111 </param>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
112 <param argument="-hm" name="homopolymer_size" type="integer" value="3" min="2"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
113 label="Homopolymer size"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
114 help="Affected plot: Homopolymer indels; sets the minimal number of consecutive bases that define a homopolymer" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
115 </section>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
116 </inputs>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
117 <outputs>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
118 <data name="output_html" format="html"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
119 label="${tool.name} report on ${on_string}" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
120 <data name="output_per_base_coverage" format="tsv"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
121 label="${tool.name} per-base coverage on ${on_string}">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
122 <filter>per_base_coverage</filter>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
123 </data>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
124 <collection name="raw_data" type="list"
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
125 label="Raw data for ${tool.name} on ${on_string}">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
126 <data name="genome_results" format="txt" from_work_dir="results/summary_report.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
127 <data name="coverage_across_reference" format="tsv" from_work_dir="results/coverage_across_reference.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
128 <data name="coverage_histogram" format="tsv" from_work_dir="results/coverage_histogram.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
129 <data name="genome_fraction_coverage" format="tsv" from_work_dir="results/genome_fraction_coverage.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
130 <data name="duplication_rate_histogram" format="tsv" from_work_dir="results/duplication_rate_histogram.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
131 <data name="mapped_reads_clipping_profile" format="tsv" from_work_dir="results/mapped_reads_clipping_profile.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
132 <data name="mapped_reads_gc-content_distribution" format="tsv" from_work_dir="results/mapped_reads_gc-content_distribution.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
133 <data name="mapped_reads_nucleotide_content" format="tsv" from_work_dir="results/mapped_reads_nucleotide_content.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
134 <data name="mapping_quality_across_reference" format="tsv" from_work_dir="results/mapping_quality_across_reference.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
135 <data name="mapping_quality_histogram" format="tsv" from_work_dir="results/mapping_quality_histogram.txt" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
136 </collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
137 </outputs>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
138 <tests>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
139 <test expect_num_outputs="12">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
140 <param name="input1" value="test_mapped_reads.bam"/>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
141 <output name="output_html" ftype="html">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
142 <assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
143 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
144 </assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
145 </output>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
146 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
147 <element name="genome_results" file="genome_results_default.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
148 </output_collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
149 </test>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
150 <test expect_num_outputs="13">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
151 <param name="input1" value="test_mapped_reads.bam" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
152 <param name="per_base_coverage" value="true" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
153 <output name="output_html" ftype="html">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
154 <assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
155 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
156 </assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
157 </output>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
158 <output name="output_per_base_coverage" file="per_base_coverage_default.txt" ftype="tsv" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
159 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
160 <element name="genome_results" file="genome_results_default.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
161 </output_collection>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
162 </test>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
163 <test expect_num_outputs="12">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
164 <param name="input1" value="test_mapped_reads.bam"/>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
165 <conditional name="stats_regions">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
166 <param name="region_select" value="custom_regions" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
167 <param name="regions" value="features.gtf" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
168 </conditional>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
169 <output name="output_html" ftype="html">
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170 <assert_contents>
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171 <has_text text="Qualimap report: BAM QC" />
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172 </assert_contents>
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173 </output>
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174 <output_collection name="raw_data" type="list">
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175 <element name="genome_results" file="genome_results_inside_features.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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176 </output_collection>
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177 </test>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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178 <test expect_num_outputs="13">
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179 <param name="input1" value="test_mapped_reads.bam" />
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180 <conditional name="stats_regions">
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181 <param name="region_select" value="custom_regions" />
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182 <param name="regions" value="features.gtf" />
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183 </conditional>
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184 <param name="per_base_coverage" value="true" />
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185 <output name="output_html" ftype="html">
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186 <assert_contents>
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187 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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188 </assert_contents>
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189 </output>
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190 <output name="output_per_base_coverage" file="per_base_coverage_inside_features.txt" ftype="tsv" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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191 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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192 <element name="genome_results" file="genome_results_inside_features.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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193 </output_collection>
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194 </test>
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195 <test expect_num_outputs="13">
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196 <param name="input1" value="test_mapped_reads.bam" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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197 <conditional name="stats_regions">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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198 <param name="region_select" value="custom_regions" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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199 <param name="regions" value="features.gtf" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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200 <param name="outside_stats" value="true" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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201 </conditional>
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202 <param name="per_base_coverage" value="true" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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203 <output name="output_html" ftype="html">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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204 <assert_contents>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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205 <has_text text="Qualimap report: BAM QC" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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206 </assert_contents>
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207 </output>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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208 <output name="output_per_base_coverage" file="per_base_coverage_outside_features.txt" ftype="tsv" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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209 <output_collection name="raw_data" type="list">
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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210 <element name="genome_results" file="genome_results_outside_features.txt" ftype="txt" compare="diff" lines_diff="2" />
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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211 </output_collection>
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212 </test>
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213 </tests>
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214 <help><![CDATA[
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215 **What it does**
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216
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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217 **Qualimap BAM QC** lets you evaluate the quality of aligned reads data in BAM
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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218 format. The tool summarizes basic statistics of the alignment (number of reads,
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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219 coverage, GC-content, etc.) and produces a number of useful graphs for their
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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220 interpretation.
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221
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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222 The analysis can be performed with any kind of sequencing data, such as
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223 whole-genome sequencing, exome sequencing, RNA-seq or ChIP-seq data.
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224
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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225 In addition, it is possible to provide an annotation file so the results are
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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226 computed for the reads mapping inside (and optionally outside) of the
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227 corresponding genomic regions, which can be especially useful for evaluating
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228 target-enrichment sequencing studies.
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229
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230 Input
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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231 =====
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232
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233 *Mapped reads input dataset*
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234
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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235 The dataset holding the mapped reads to carry out the analysis with.
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236
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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237 *Dataset specifying regions*
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238
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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239 If you decide to calculate mapping statistics for selected regions of the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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240 reference genome (instead of for the whole genome), you need to specify the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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241 regions through this additional dataset in gtf, gff or bed format.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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242
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243 .. class:: infomark
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244
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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245 A typical problem when working with regions (and genome annotation data, in general) is potential inconsistency between the chromosome names used in the mapped reads input versus those used to define the regions. In the case of the human genome, for example, UCSC data has chromosomes starting with a 'chr' prefix, which is lacking from Ensemble data. This simple form of the problem is handled by Qualimap: if chromosome names in the regions input have a 'chr' prefix, Qualimap will add that prefix to the mapped reads chromosome names as needed. For more complex cases you will have to adjust your inputs manually.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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246
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247
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248 Parameters
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249 ----------
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250
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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251 *Reference genome regions to calculate mapping statistics for*
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252
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253 Choose whether you would like to have mapping statistics reported across
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254
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255 - the entire reference genome
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256 (as specified in the header of the mapped reads input)
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257
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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258 - specific regions of the reference
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259
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260 In the second case, you need to select a *Dataset specifying regions* (see
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261 above). Using the *Invert regions* switch you can then indicate whether you
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262 want to select or exclude the regions in this dataset.
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263
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264 *Generate per-base coverage output*
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265
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266 *Skip duplicate reads*
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267
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268 The tool lets you skip alignments of duplicate reads from the analysis.
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269 Depending on whether you select none, either one, or both of the available
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270 options, you can decide to:
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271
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272 - not correct for duplicate reads at all (*e.g.* because you have removed them
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273 at an earlier step with some dedicated tool)
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274 - identify and flag duplicate reads with a dedicated tool (like ``Picard
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275 MarkDuplicates`` or ``samtools markdup``), then have Qualimap ignore the
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276 duplicate-flagged reads (recommended, most flexible option since other tools
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277 can be told to ignore the same reads)
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278 - have Qualimap identify potential duplicates by itself and ignore them
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279 - combine external and Qualimap-internal duplicate detection for extra
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280 stringency
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281
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282 Independent of your selection, the HTML report will always list (in the
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283 `Globals` section of the `Summary`) the number of duplicated reads estimated by
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284 Qualimap. If you choose to skip duplicates, you will also be informed about the
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285 number of skipped reads in that same section and, if you instruct Qualimap to
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286 look for the duplicate flag on reads, the number of reads flagged as duplicates
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287 will also be reported here.
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288
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289 **Section: Settings affecting specific plots**
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290
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291 Parameters in this section only affect some (or even only one) of the plots
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292 contained in the HTML report (and the corresponding part of the *Raw Data*
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293 output collection).
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294
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295 For most of these options, the parameter help above should be descriptive
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296 enough. Just a few more words on two of them:
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297
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298 *Number of bins to use in across-reference plots*
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299
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300 This value is used for computing the various graphs that plot information
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301 across the reference. Basically, the reference genome gets split into the given
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302 number of bins, and reads falling in the same bin are aggregated in the
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303 statistics of that bin.
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304
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305 Thus, the higher the number of bins, the higher the resolution of the plots,
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306 but more bins also require longer time for their statistics to be computed.
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307 Less bins, on the other hand, mean more reads will have to be aggregated per
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308 bin and this comes with higher memory requirements. Hence, if the tool fails
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309 with an ``Out Of Memory`` error, you may want to rerun it with a higher bin
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310 number.
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311
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312 *Plot expected GC-content distribution of the following reference genome*
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313
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314 The choice of reference genomes with pre-calculated GC distributions is built
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315 into Qualimap.
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316
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317 Future releases of Qualimap may include more choices, but the current version
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318 is limited to those offered here.
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319
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320
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321 Outputs
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322 =======
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323
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324 HTML Report
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325 -----------
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326
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327 **Summary Section**
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328
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329 *Globals*
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330
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331 This section contains information about the total number of reads, number of mapped reads, paired-end mapping performance, read length distribution,
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
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332 number of clipped reads and duplication rate (estimated from the start positions of read alignments).
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333
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334 *ACGT Content*
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335
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336 Nucleotide content and GC percentage in the mapped reads.
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337
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338 *Coverage*
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339
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340 Mean and standard deviation of the coverage depth.
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341
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342 *Mapping quality*
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343
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344 Mean mapping quality of the mapped reads.
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345
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346 *Insert size*
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347
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348 Mean, standard deviation and percentiles of the insert size distribution if applicable. The features are computed based on the TLEN field of the SAM file.
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349
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350 *Mismatches and indels*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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351
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
352 The section reports general alignment error rate (computed as a ratio of total collected edit distance to the number of mapped bases), total number of mismatches and total number of indels (computed from the CIGAR values). Additionally fraction of the homopolymer indels among total indels is provided. Note, the error rate and mismatches metrics are based on optional fields of a SAM record (NM for edit distance, MD for mismatches). The features are not reported if these fields are missing in the SAM file.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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353
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
354 *Chromosome stats*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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355
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
356 Number of mapped bases, mean and standard deviation of the coverage depth for each chromosome as defined by the header of the SAM file.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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357
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
358 For region-based analysis the information is given inside of regions, including some additional information like, for example, number of correct strand reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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359
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
360
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
361 **Plots**
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
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362
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
363 *Coverage Across Reference*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
364
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
365 This plot consists of two figures.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
366 The upper figure provides the coverage distribution (red line) and coverage
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
367 deviation across the reference sequence.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
368 The lower figure shows GC content across reference (black line) together with
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
369 its average value (red dotted line).
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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370
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
371 *Coverage Histogram*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
372
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
373 Histogram of the number of genomic locations having a given coverage rate.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
374 The bins of the x-axis are conveniently scaled by aggregating some coverage
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
375 values in order to produce a representative histogram also in presence of the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
376 usual NGS peaks of coverage.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
377
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
378 *Coverage Histogram (0-50X)*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
379
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
380 Similar to the previous plot, but in this graph genome locations with a
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
381 coverage greater than 50X are grouped into the last bin.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
382 By doing so a higher resolution of the most common values for the coverage rate
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
383 is obtained.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
384
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
385 *Genome Fraction Coverage*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
386
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
387 Provides a visual way of knowing how much reference has been sequenced to at
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
388 least a given coverage rate.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
389 This graph should be interpreted as in this example:
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
390 If one aims for a coverage rate of at least 25X (x-axis), how much of the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
391 reference (y-axis) will be considered?
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
392
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
393 *Duplication Rate Histogram*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
394
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
395 This plot shows the distribution of duplicated reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
396 Due to several factors (*e.g.* amount of starting material, sample preparation,
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
397 *etc.*) it is possible that the same fragments are sequenced several times.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
398 For some experiments where enrichment is used (*e.g.* ChIP-seq ) this is
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
399 expected to some degree.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
400 For most experiments, however, a high duplication level of the reads indicates
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
401 some unwanted bias.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
402
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
403 *Mapped Reads Nucleotide Content*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
404
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
405 This plot shows the nucleotide content per position of the mapped reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
406
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
407 *Mapped Reads GC Content Distribution*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
408
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
409 This graph shows the distribution of GC-content per mapped read.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
410 If compared with a precomputed genome distribution, this plot allows to check
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
411 if there is a shift in the GC content.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
412
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
413 *Mapped Reads Clipping Profile*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
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414
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
415 Represents the percentage of clipped bases across the reads.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
416 Technically, the clipping is detected via SAM format CIGAR codes ‘H’
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
417 (hard clipping) and ‘S’ (soft clipping).
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
418 In addition, the total number of clipped reads can be found in the report
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
419 `Summary` section.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
420
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
421 This plot is not shown if no clipped reads are found.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
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422
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
423 *Homopolymer Indels*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
424
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
425 This bar plot shows the number of indels that are located within A, C, G and T
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
426 homopolymers, respectively, as well as the number of indels that are not within
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
427 any homopolymer. Large numbers of homopolymer indels may indicate a problem in
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
428 the sequencing process.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
429 Technically, Qualimap identifies indels from the CIGAR code of the aligned
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
430 reads. Indel statistics can also be found in a dedicated section of the report
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
431 `Summary`.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
432
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
433 This graph is not shown if the sample doesn’t contain any indels.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
434
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
435 *Mapping Quality Across Reference*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
436
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
437 This plot provides the mapping quality distribution across the reference.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
438 To construct the plot, the mean mapping quality is computed for each bin.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
439
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
440 *Mapping Quality Histogram*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
441
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
442 Histogram of the number of genomic locations having a given mapping quality.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
443 To construct the histogram the mean mapping quality is computed at each genome
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
444 position with non-zero coverage and collected.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
445 According to the SAM/BAM format specifications, the range for the mapping
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
446 quality score is [0-255].
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
447
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
448 *Insert Size Across Reference*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
449
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
450 This plot provides the insert size distribution across the reference.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
451 Technically, the insert size of each pair of aligned reads is collected from
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
452 the SAM alignment field `TLEN`. Only positive values are taken into account.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
453 To construct the plot, the mean insert size is computed for each bin.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
454
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
455 *Insert Size Histogram*
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
456
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
457 Histogram of insert size distribution.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
458
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
459
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
460 Raw Data
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
461 --------
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
462
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
463 This is a *Collection* of 10 individual datasets.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
464
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
465 The *genome_results* dataset provides a plain-text summary of key statistics,
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
466 most of which can also be found in the *Summary* section of the *HTML Report*.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
467
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
468 The remaining 9 datasets hold the tabular raw data underlying the plots of the corresponding names in the *HTML Report*.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
469
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
470
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
471 Per-base coverage
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
472 -----------------
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
473
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
474 Optional. This is a tabular dataset listing the coverage of every base in the
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
475 reference genome unless that coverage is zero. Since its content is
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
476 uncompressed text, this dataset can easily become huge, and it is recommended
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
477 that you generate this dataset only for very small genomes or very limited
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
478 regions of larger genomes.
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
479 ]]> </help>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
parents:
diff changeset
480 <expand macro="citations"/>
ac607906f10a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/qualimap commit b4d43001cc0caa14d760c347fa1c416929f769b2"
iuc
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diff changeset
481 </tool>