annotate ragtag.xml @ 0:a04e64efa43a draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
author iuc
date Wed, 10 Nov 2021 23:33:13 +0000
parents
children d110a4141898
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rev   line source
0
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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1 <tool id='ragtag' name='RagTag' version='@TOOL_VERSION@+galaxy@VERSION_SUFFIX@' profile='20.01'>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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2 <description>reference-guided scaffolding of draft genomes</description>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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3 <macros>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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4 <import>macros.xml</import>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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5 </macros>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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6 <expand macro='xrefs' />
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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7 <expand macro='requirements' />
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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8 <command detect_errors='exit_code'><![CDATA[
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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9 #if $mode_conditional.mode_option != 'merge'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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10 #if $mode_conditional.advanced_options.mapping_conditional.mapping_option == 'nucmer'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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11 #set $nucmer_params = '%s -l %s -c %s' % ($mode_conditional.advanced_options.mapping_conditional.anchor_mode,
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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12 $mode_conditional.advanced_options.mapping_conditional.l,
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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13 $mode_conditional.advanced_options.mapping_conditional.c)
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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14 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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15 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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16 #if $mode_conditional.mode_option == 'merge'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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17 #set $input_files = list()
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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18 mkdir merge_files &&
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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19 #for $i, $j in enumerate($mode_conditional.scaffold_files)
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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20 #set $out_file = './merge_files/scaffold_%s.agp' % $i
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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21 ln -s '${j}' $out_file &&
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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22 $input_files.append($out_file)
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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23 #end for
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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24 #set $merge_files = " ".join($input_files)
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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25 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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26 ragtag.py $mode_conditional.mode_option -u
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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27 #if $mode_conditional.mode_option == 'correct'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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28 @INPUTS@
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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29 @COMMON_PARAMETERS@
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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30 #if $mode_conditional.validation_conditional.validation_option == 'true'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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31 -R '${mode_conditional.validation_conditional.R}'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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32 -T $mode_conditional.validation_conditional.read_type
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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33 -v $mode_conditional.validation_conditional.v
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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34 #if $mode_conditional.validation_conditional.max_cov
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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35 --max-cov $mode_conditional.validation_conditional.max_cov
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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36 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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37 #if $mode_conditional.validation_conditional.min_cov
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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38 --min-cov $mode_conditional.validation_conditional.min_cov
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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39 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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40 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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41 -b $mode_conditional.advanced_options.b
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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42 #if $mode_conditional.advanced_options.missasembly_break
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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43 $mode_conditional.advanced_options.missasembly_break
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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44 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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45 #if $mode_conditional.advanced_options.gff
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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46 --gff '${mode_conditional.advanced_options.gff}'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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47 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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48 --read-aligner 'minimap2' ## it is the only allowed
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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49 #else if $mode_conditional.mode_option == 'scaffold'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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50 @INPUTS@
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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51 @COMMON_PARAMETERS@
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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52 -i $mode_conditional.advanced_options.i
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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53 -a $mode_conditional.advanced_options.a
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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54 -s $mode_conditional.advanced_options.s
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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55 #if $mode_conditional.advanced_options.gap_conditional.gap_option == 'true'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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56 -r
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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57 -g '${mode_conditional.advanced_options.gap_conditional.g}'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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58 -m '${mode_conditional.advanced_options.gap_conditional.m}'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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59 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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60 #if $mode_conditional.advanced_options.unplaced_conditional.unplaced_option == 'true'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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61 -C
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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62 #if $mode_conditional.advanced_options.unplaced_conditional.J
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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63 -J '${mode_conditional.advanced_options.unplaced_conditional.J}'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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64 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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65 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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66 #else if $mode_conditional.mode_option == 'patch'
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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67 @INPUTS@
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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68 @COMMON_PARAMETERS@
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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69 -s $mode_conditional.advanced_options.s
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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70 -i $mode_conditional.advanced_options.i
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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71 #if $mode_conditional.advanced_options.patching_mode
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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72 $mode_conditional.advanced_options.patching_mode
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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73 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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74 #else
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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75 $assembly_fasta
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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76 #if $mode_conditional.scaffold_files
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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77 $merge_files
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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78 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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79 #if $mode_conditional.merging_options.j
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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80 -j $mode_conditional.merging_options.j
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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81 #end if
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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82 -l $mode_conditional.merging_options.l
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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83 -e $mode_conditional.merging_options.e
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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84 --gap-func $mode_conditional.merging_options.function_merging
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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85 #if $mode_conditional.hic_options.b
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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86 -b $mode_conditional.hic_options.b
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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87 -r $mode_conditional.hic_options.r
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88 -p $mode_conditional.hic_options.p
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89 #end if
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90 #end if
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91 -o ./
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92 #if $mode_conditional.mode_option != 'merge'
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93 -t \${GALAXY_SLOTS:-2}
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94 #end if
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95 #if $mode_conditional.mode_option == 'patch'
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96 && mv ragtag.patch.asm.paf.log ragtag.patch.log
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97 #end if
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98 ]]> </command>
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99 <inputs>
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100 <conditional name="mode_conditional">
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101 <param name="mode_option" type="select" label="Operation mode">
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102 <option value="correct">Correct: homology-based missasembly correction</option>
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103 <option value="scaffold">Scaffold: homology-based assebly scaffolding</option>
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104 <option value="patch">Patch: homology-based assembly patching</option>
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105 <option value="merge">Merge: scaffolding merging</option>
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106 </param>
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107 <when value="correct">
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108 <expand macro="input_options"/>
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109 <conditional name="validation_conditional">
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110 <param name="validation_option" type="select" label="Use validation reads">
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111 <option value="true">Enabled</option>
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112 <option value="false" selected="true">Disabled</option>
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113 </param>
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114 <when value="true">
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115 <param argument="-R" type="data" format="fastq,fastqsanger" label="Validation reads"
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116 help="Without validation, the module will break at any point of reference discordance as defined by the 'correction options'.
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117 With validation, RagTag maps reads to the query assembly and verifies putative break points if they are near regions of
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118 exceptionally low or high coverage. The reads used for validation should come from the same genotype as the query
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119 assembly to ensure that coverage abnormalities don't arise from true biological variation" />
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120 <param name="read_type" type="select" label="Read type">
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121 <option value="sr">Illumina</option>
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122 <option value="ont">Nanopore</option>
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123 <option value="corr">Error corrected long-reads</option>
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124 </param>
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125 <param argument="-v" type="integer" min="0" value="10000" label="Coverage validation window size"
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126 help="This parameter specifies the window around the putative misassembly break point that RagTag examines
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127 for exceptionally low or high read coverage. The larger this window size, the more likely
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128 it is to find an unrelated coverage abnormality"/>
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129 <param argument="--max-cov" type="integer" min="0" value="" optional="true" label="Break sequences at regions at or above this coverage level"/>
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130 <param argument="--min-cov" type="integer" min="0" value="" optional="true" label="Break sequences at regions at or below this coverage level"/>
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131 </when>
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132 <when value="false"/>
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133 </conditional>
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134 <section name="advanced_options" title="Advanced options">
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135 <expand macro="common_parameters"/>
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136 <param argument="-b" type="integer" min="0" value="5000" label="Minimum break distance from contig ends"
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137 help="Breaks will not be made within -b bp of query sequence termini"/>
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138 <param name="missasembly_break" type="select" optional="true" label="Break misassebly option"
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139 help="One can also direct RagTag to only break misassemblies between (--inter, query maps to >1 reference sequence) or within
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140 (--intra, query maps discordantly to 1 reference sequence) reference sequences">
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141 <option value="--inter">Only break misassemblies between reference sequences (--inter)</option>
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142 <option value="--intra">Only break missasemblies within reference sequences (--intra)</option>
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143 </param>
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144 <param argument="--gff" type="data" format="gff" optional="true" label="Don't break sequences within GFF intervals"
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145 help=" If one has annotations associated with the query assembly, provide them with the --gff option to ensure that the query assembly
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146 is never broken within annotation intervals. "/>
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147 </section>
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148 <param name="output_correct" type="select" multiple="true" label="Output files">
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149 <option value="fasta" selected="true">The corrected query assembly in FASTA format</option>
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150 <option value="agp" selected="true">The AGP file defining the exact coordinates of query sequence breaks</option>
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151 <option value="paf">The description of the approximate mapping positions between two set of sequences in PAF format</option>
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152 <option value="log">Log file</option>
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153 </param>
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154 </when>
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155 <when value="scaffold">
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156 <expand macro="input_options"/>
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157 <section name="advanced_options" title="advanced options">
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158 <expand macro="common_parameters"/>
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159 <param argument="-i" type="float" min="0" max="1" value="0.2" label="Minimum grouping confidence score"
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160 help="The grouping confidence score is the number of base pairs a contig covered in its assigned reference chromosome
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161 divided by the total number of covered base pairs in the entire reference genome"/>
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162 <param argument="-a" type="float" min="0" max="1" value="0" label="Minimum location confidence score"
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163 help="To create a metric associated with contig ordering confidence, Ragtag define a location confidence. First, the smallest
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164 and largest alignment positions, with respect to the reference, between a contig and its assigned reference chromosome are found.
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165 The location confidence is then calculated as the number of covered base pairs in this range divided by the total number of
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166 base pairs in the range"/>
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167 <param argument="-s" type="float" min="0" max="1" value="0" label="Minimum orientation confidence score"
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168 help="To calculate the orientation confidence, each base pair in each alignment between a contig and its assigned reference chromosome
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169 casts a vote for the orientation of its alignment. The orientation confidence is the number of votes for the assigned orientation of
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170 the contig divided by the total number of votes"/>
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171 <conditional name="gap_conditional">
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parents:
diff changeset
172 <param name="gap_option" type="select" label="Infer gap sizes" help="When disabled, all gaps are 100 bp (-r)">
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parents:
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173 <option value="true" selected="true">Enabled</option>
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iuc
parents:
diff changeset
174 <option value="false">Disabled</option>
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iuc
parents:
diff changeset
175 </param>
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iuc
parents:
diff changeset
176 <when value="true">
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iuc
parents:
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177 <param argument="-g" type="integer" min="0" value="100" label="Minimum infered gap size" />
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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parents:
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178 <param argument="-m" type="integer" min="0" value="100000" label="Maximum inferred gap size"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
179 </when>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
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180 <when value="false"/>
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iuc
parents:
diff changeset
181 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
182 <conditional name="unplaced_conditional">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
183 <param name="unplaced_option" type="select" label="Concatenate unplaced contigs and make 'chr0' (-C)">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
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184 <option value="true">Enabled</option>
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iuc
parents:
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185 <option value="false" selected="true">Disabled</option>
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iuc
parents:
diff changeset
186 </param>
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iuc
parents:
diff changeset
187 <when value="true">
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parents:
diff changeset
188 <param argument="-J" type="data" format="txt" optional="true" label="List of query headers to leave unplaceds and exclude form 'chr0'"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
189 </when>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
190 <when value="false"/>
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parents:
diff changeset
191 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
192 </section>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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parents:
diff changeset
193 <param name="output_scaffold" type="select" multiple="true" label="Output files">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
194 <option value="fasta" selected="true">The scaffolds in FASTA format, defined by the ordering and orientations of the sequences containted in the AGP file</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
195 <option value="agp" selected="true">The ordering and orientations of query sequences in AGP format</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
196 <option value="paf">The description of the approximate mapping positions between two set of sequences in PAF format</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
197 <option value="confidence">Confidence score values</option>
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iuc
parents:
diff changeset
198 <option value="stats">Summary statistics for the scaffolding process</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
199 <option value="log">Log file</option>
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parents:
diff changeset
200 </param>
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iuc
parents:
diff changeset
201 </when>
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iuc
parents:
diff changeset
202 <when value="patch">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
203 <expand macro="input_options"/>
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iuc
parents:
diff changeset
204 <section name="advanced_options" title="advanced options">
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iuc
parents:
diff changeset
205 <expand macro="common_parameters"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
206 <param argument="-s" type="integer" min="0" value="50000" label="Minimum merged alignment length"
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
207 help="After merging, alignments less than -s bp long will be removed"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
208 <param argument="-i" type="float" min="0" max="1" value="0.05" label="Maximum merged alignment distance"
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
209 help="Maximum merged alignment distance from sequence terminus as fraction of the sequence length. Alignments must
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
210 be within -i bp of a target sequence terminus or gap to be considered for patchin "/>
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iuc
parents:
diff changeset
211 <param name="patching_mode" type="select" optional="true" label="Patching mode">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
212 <option value="--fill-only">Only fill existing target gaps. Do not join target sequences</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
213 <option value="--join_only">Only join and patch target sequences. DO not fill existing gaps</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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diff changeset
214 </param>
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iuc
parents:
diff changeset
215 </section>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
216 <param name="output_patch" type="select" multiple="true" label="Output files">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
217 <option value="final_fasta" selected="true">The final FASTA file containing the patched assembly</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
218 <option value="final_agp" selected="true">The final AGP file defining how final FASTA is built</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
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diff changeset
219 <option value="assembly_file" selected="true">Assembly alignment files</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
220 <option value="split_assembly">The split target assembly and the renamed query assembly combined into one FASTA file</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
221 <option value="split_description">An AGP file defining how the target assembly was split at gaps</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
222 <option value="target_gaps">The target assembly split at gaps</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
223 <option value="agp_renamed">An AGP file defining the new names for query sequences</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
224 <option value="fasta_renamed">A FASTA file with the original query sequence, but with new names</option>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
225 <option value="log">Log file</option>
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diff changeset
226 </param>
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diff changeset
227 </when>
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iuc
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228 <when value="merge">
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iuc
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diff changeset
229 <param name="assembly_fasta" type="data" format="fasta" label="Assembly FASTA file"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
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diff changeset
230 <param name="scaffold_files" type="data" format="agp" multiple="true" optional="true" label="Scaffold AGP files"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
231 <section name="merging_options" title="Merging options">
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diff changeset
232 <param argument="-j" type="data" format="txt" optional="true" label="List of query headers to leave unplaced"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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233 <param argument="-l" type="integer" min="0" value="100000" label="Minimum assembly sequence length"
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
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234 help="Assembly sequences shorter than -l will also be left unplaced."/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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parents:
diff changeset
235 <param argument="-e" type="float" min="0" value="0" label="Minimum edge weight"
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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diff changeset
236 help="The edges in the merging graph represent scaffolding adjacencies. If an AGP file supports a particular adjacency,
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
237 its weight is added to the edge weight. Any edges with a weight lower than the minimum edge weigth will be removed from the graph"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
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diff changeset
238 <param name="function_merging" type="select" label="Function for merging gap lengths"
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
239 help="Scaffold gaps can differ between input AGP files. For example, a Hi-C derived AGP file might place 100 bp gaps between sequences
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
240 while a reference-guided AGP file might infer gap sizes based on a reference genome. Use this parameter to specify how gap sizes
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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parents:
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241 should be computed from the supporting AGP files (--gap-func)">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
242 <option value="min" selected="true">Min</option>
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iuc
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diff changeset
243 <option value="max">Max</option>
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iuc
parents:
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244 <option value="mean">Mean</option>
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iuc
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diff changeset
245 </param>
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iuc
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diff changeset
246 </section>
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iuc
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247 <section name="hic_options" title="HI-C options">
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iuc
parents:
diff changeset
248 <param argument="-b" type="data" format="bam" optional="true" label="Hi-C alignments" help="Sorted by read name"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
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249 <param argument="-r" type="text" value="" optional="true" label="Restriction enzymes/sites or 'DNase'" help="List of restrction enzimes/sites or 'DNase', separated by comma. E.g. GATC,GACC">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
250 <sanitizer invalid_char="">
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iuc
parents:
diff changeset
251 <valid initial="string.letters,string.digits">
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iuc
parents:
diff changeset
252 <add value="," />
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iuc
parents:
diff changeset
253 <add value="[" />
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iuc
parents:
diff changeset
254 <add value="]" />
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
255 </valid>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
256 </sanitizer>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
257 <validator type="regex">[0-9a-zA-Z,\]\[]+</validator>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
258 </param>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
259 <param argument="-p" type="float" min="0" max="1" value="1" optional="true" label="Portion of the sequence termini to consider for links"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
260 </section>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
261 </when>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
262 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
263 </inputs>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
264 <outputs>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
265 <!--Correct mode outputs-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
266 <data format="paf" name="correct_paf" from_work_dir="ragtag.correct.asm.paf" label="${tool.name} on ${on_string}: PAF">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
267 <filter>mode_conditional["mode_option"] == "correct" and "paf" in mode_conditional["output_correct"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
268 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
269 <data format="agp" name="correct_agp" from_work_dir="ragtag.correct.agp" label="${tool.name} on ${on_string}: AGP">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
270 <filter>mode_conditional["mode_option"] == "correct" and "agp" in mode_conditional["output_correct"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
271 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
272 <data format="fasta" name="correct_fasta" from_work_dir="ragtag.correct.fasta" label="${tool.name} on ${on_string}: FASTA">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
273 <filter>mode_conditional["mode_option"] == "correct" and "fasta" in mode_conditional["output_correct"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
274 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
275 <data format="txt" name="correct_log" from_work_dir="ragtag.correct.asm.paf.log" label="${tool.name} on ${on_string}: log">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
276 <filter>mode_conditional["mode_option"] == "correct" and "log" in mode_conditional["output_correct"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
277 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
278 <!--Scaffold mode outputs-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
279 <data format="paf" name="scaffold_paf" from_work_dir="ragtag.scaffold.asm.paf" label="${tool.name} on ${on_string}: PAF">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
280 <filter>mode_conditional["mode_option"] == "scaffold" and "paf" in mode_conditional["output_scaffold"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
281 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
282 <data format="agp" name="scaffold_agp" from_work_dir="ragtag.scaffold.agp" label="${tool.name} on ${on_string}: AGP">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
283 <filter>mode_conditional["mode_option"] == "scaffold" and "agp" in mode_conditional["output_scaffold"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
284 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
285 <data format="fasta" name="scaffold_fasta" from_work_dir="ragtag.scaffold.fasta" label="${tool.name} on ${on_string}: FASTA">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
286 <filter>mode_conditional["mode_option"] == "scaffold" and "fasta" in mode_conditional["output_scaffold"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
287 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
288 <data format="txt" name="scaffold_log" from_work_dir="ragtag.scaffold.asm.paf.log" label="${tool.name} on ${on_string}: log">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
289 <filter>mode_conditional["mode_option"] == "scaffold" and "log" in mode_conditional["output_scaffold"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
290 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
291 <data format="tabular" name="scaffold_stats" from_work_dir="ragtag.scaffold.stats" label="${tool.name} on ${on_string}: stats">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
292 <filter>mode_conditional["mode_option"] == "scaffold" and "stats" in mode_conditional["output_scaffold"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
293 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
294 <data format="tabular" name="scaffold_confidence" from_work_dir="ragtag.scaffold.confidence.txt" label="${tool.name} on ${on_string}: confidence">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
295 <filter>mode_conditional["mode_option"] == "scaffold" and "confidence" in mode_conditional["output_scaffold"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
296 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
297 <!--Patch mode outputs-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
298 <data format="agp" name="patch_agp" from_work_dir="ragtag.patch.agp" label="${tool.name} on ${on_string}: final AGP">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
299 <filter>mode_conditional["mode_option"] == "patch" and "final_agp" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
300 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
301 <data format="paf" name="patch_paf" from_work_dir="ragtag.patch.asm.paf" label="${tool.name} on ${on_string}: final PAF">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
302 <filter>mode_conditional["mode_option"] == "patch" and "assembly_file" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
303 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
304 <data format="txt" name="patch_log" from_work_dir="ragtag.patch.log" label="${tool.name} on ${on_string}: log">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
305 <filter>mode_conditional["mode_option"] == "patch" and "log" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
306 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
307 <data format="fasta" name="patch_comps_fasta" from_work_dir="ragtag.patch.comps.fasta" label="${tool.name} on ${on_string}: components FASTA">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
308 <filter>mode_conditional["mode_option"] == "patch" and "split_assembly" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
309 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
310 <data format="agp" name="patch_ctg_agp" from_work_dir="ragtag.patch.ctg.agp" label="${tool.name} on ${on_string}: contigs AGP">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
311 <filter>mode_conditional["mode_option"] == "patch" and "split_description" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
312 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
313 <data format="fasta" name="patch_ctg_fasta" from_work_dir="ragtag.patch.ctg.fasta" label="${tool.name} on ${on_string}: contigs FASTA">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
314 <filter>mode_conditional["mode_option"] == "patch" and "target_gaps" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
315 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
316 <data format="fasta" name="patch_fasta" from_work_dir="ragtag.patch.fasta" label="${tool.name} on ${on_string}: final FASTA">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
317 <filter>mode_conditional["mode_option"] == "patch" and "final_fasta" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
318 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
319 <data format="agp" name="patch_rename_agp" from_work_dir="ragtag.patch.rename.agp" label="${tool.name} on ${on_string}: renamed AGP">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
320 <filter>mode_conditional["mode_option"] == "patch" and "agp_renamed" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
321 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
322 <data format="fasta" name="patch_rename_fasta" from_work_dir="ragtag.patch.rename.fasta" label="${tool.name} on ${on_string}: renamed FASTA">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
323 <filter>mode_conditional["mode_option"] == "patch" and "fasta_renamed" in mode_conditional["output_patch"]</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
324 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
325 <!-- Merge mode outputs-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
326 <data format="agp" name="merge_agp" from_work_dir="ragtag.merge.agp" label="${tool.name} on ${on_string}: merged AGP">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
327 <filter>mode_conditional["mode_option"] == "merge"</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
328 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
329 <data format="fasta" name="merge_fasta" from_work_dir="ragtag.merge.fasta" label="${tool.name} on ${on_string}: merged FASTA">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
330 <filter>mode_conditional["mode_option"] == "merge"</filter>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
331 </data>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
332 </outputs>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
333 <tests>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
334 <test expect_num_outputs="4">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
335 <!--Test 01 correct mode minimap2-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
336 <conditional name="mode_conditional">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
337 <param name="mode_option" value="correct"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
338 <param name="reference" value="genome.fna"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
339 <param name="query" value="contigs.fna"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
340 <param name="output_correct" value="fasta,agp,paf,log"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
341 <section name="advanced_options">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
342 <param name="e" value="reference_headers_skip.txt"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
343 <param name="j" value="query_headers_skip.txt"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
344 <param name="f" value="1000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
345 <conditional name="mapping_conditional">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
346 <param name="mapping_option" value="minimap2"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
347 <param name="mm2_params" value="asm5"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
348 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
349 <param name="remove_small" value="false"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
350 <param name="q" value="10"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
351 <param name="d" value="100000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
352 <param name="b" value="5000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
353 <param name="missasembly_break" value="--inter"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
354 <param name="gff" value="annotation.gff"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
355 </section>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
356 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
357 <output name="correct_paf" file="correct_paf_01.paf" ftype="paf"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
358 <output name="correct_agp" file="correct_agp_01.agp" ftype="agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
359 <output name="correct_fasta" file="correct_fasta_01.fasta" ftype="fasta"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
360 <output name="correct_log" file="correct_log_01.txt" ftype="txt" lines_diff="20"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
361 </test>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
362 <!--Test 02 correct mode nucmer-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
363 <test expect_num_outputs="2">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
364 <conditional name="mode_conditional">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
365 <param name="mode_option" value="correct"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
366 <param name="reference" value="genome.fna"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
367 <param name="query" value="contigs.fna"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
368 <param name="output_correct" value="fasta,agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
369 <section name="advanced_options">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
370 <param name="f" value="1000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
371 <conditional name="mapping_conditional">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
372 <param name="mapping_option" value="nucmer"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
373 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
374 <param name="remove_small" value="true"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
375 <param name="q" value="10"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
376 <param name="d" value="100000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
377 <param name="b" value="5000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
378 <param name="missasembly_break" value="--inter"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
379 </section>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
380 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
381 <output name="correct_fasta" file="correct_fasta_02.fasta" ftype="fasta"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
382 <output name="correct_agp" file="correct_agp_02.agp" ftype="agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
383
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
384 </test>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
385 <!--Test 03 scaffold mode-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
386 <test expect_num_outputs="6">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
387 <conditional name="mode_conditional">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
388 <param name="mode_option" value="scaffold"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
389 <param name="reference" value="genome.fna"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
390 <param name="query" value="contigs.fna"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
391 <param name="output_scaffold" value="fasta,agp,paf,confidence,log,stats"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
392 <section name="advanced_options">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
393 <param name="f" value="1000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
394 <param name="remove_small" value="true"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
395 <param name="q" value="10"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
396 <param name="d" value="100000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
397 <param name="i" value="0.2"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
398 <param name="a" value="0"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
399 <param name="s" value="0"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
400 </section>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
401 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
402 <output name="scaffold_paf" file="scaffold_paf_03.paf" ftype="paf"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
403 <output name="scaffold_agp" file="scaffold_apg.03.agp" ftype="agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
404 <output name="scaffold_fasta" file="scaffold_fasta_03.fasta" ftype="fasta"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
405 <output name="scaffold_log" file="scaffold_log_03.txt" ftype="txt" lines_diff="20"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
406 <output name="scaffold_stats" file="scaffold_stats_03.tabular" ftype="tabular"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
407 <output name="scaffold_confidence" file="scaffold_confidence_03.tabular" ftype="tabular"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
408 </test>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
409 <!--Test 04 patch mode-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
410 <test expect_num_outputs="9">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
411 <conditional name="mode_conditional">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
412 <param name="mode_option" value="patch"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
413 <param name="reference" value="genome.fna"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
414 <param name="query" value="contigs.fna"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
415 <param name="output_patch" value="final_fasta,final_agp,assembly_file,split_assembly,split_description,target_gaps,agp_renamed,fasta_renamed,log"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
416 <section name="advanced_options">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
417 <param name="s" value="50000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
418 <param name="i" value="0.05"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
419 </section>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
420 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
421 <output name="patch_agp" file="patch_agp_04.agp" ftype="agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
422 <output name="patch_paf" file="patch_paf_04.paf" ftype="paf"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
423 <output name="patch_log" file="patch_log_04.txt" ftype="txt" lines_diff="20"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
424 <output name="patch_comps_fasta" ftype="fasta">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
425 <assert_contents>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
426 <has_size value="603691" delta="100" />
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
427 </assert_contents>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
428 </output>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
429 <output name="patch_ctg_fasta" file="patch_ctg_fasta_04.fasta" ftype="fasta"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
430 <output name="patch_ctg_agp" file="patch_ctg_fasta_04.agp" ftype="agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
431 <output name="patch_fasta" file="patch_fasta_04.fasta" ftype="fasta"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
432 <output name="patch_rename_agp" file="patch_rename_agp.agp" ftype="agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
433 <output name="patch_rename_fasta" file="patch_rename_fasta.fasta" ftype="fasta"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
434 </test>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
435 <test expect_num_outputs="2">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
436 <!-- Test 05 merge mode-->
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
437 <conditional name="mode_conditional">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
438 <param name="mode_option" value="merge"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
439 <param name="assembly_fasta" value="correct_fasta_01.fasta"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
440 <param name="scaffold_files" value="correct_agp_01.agp,correct_agp_02.agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
441 <section name="merging_options">
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
442 <param name="l" value="100000"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
443 <param name="e" value="0"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
444 <param name="function_merging" value="min"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
445 </section>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
446 </conditional>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
447 <output name="merge_agp" file="merge_agp_05.agp" ftype="agp"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
448 <output name="merge_fasta" file="merge_fasta_05.fasta" ftype="fasta"/>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
449 </test>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
450 </tests>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
451 <help><![CDATA[
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
452 .. class:: infomark
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
453
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
454 **Purpose**
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
455
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
456 RagTag is a collection of software tools for scaffolding and improving modern genome assemblies. Tasks include:
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
457
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
458 - Homology-based misassembly correction
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
459 - Homology-based assembly scaffolding and patching
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
460 - Scaffold merging
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
461
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
462 ----
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
463
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
464 .. class:: infomark
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
465
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
466 **Correct mode**
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
467
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
468 RagTag offers a correction module that uses a reference genome to identify and correct potential misassemblies in a query assembly.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
469 RagTag also provides the option to verify putative misassemblies by aligning reads (from the same genotype) to the query assembly
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
470 and observing read coverage near misassembly break points. In all cases, sequence is never added or subtracted. Query sequences
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
471 are only broken at points of putative misassembly.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
472
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
473 *Misassemblies vs true variation*
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
474
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
475 Reference-guided misassembly signatures are sometimes caused by true biological structural variation if the reference and query assemblies
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
476 represent distinct genotypes (or haplotypes). The read validation feature should help to avoid some of these misassembly false positives,
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
477 and the validation sensitivity can be tuned with command line parameters. However, it is ultimately up to the discretion of the user to decide
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
478 if misassembly correction is appropriate. One should validate all RagTag results with independent data (usually physical, optical, or genetic
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
479 maps), when possible.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
480
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
481 ----
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
482
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
483 .. class:: infomark
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
484
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
485 **Scaffold mode**
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
486
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
487 Scaffolding is the process of ordering and orienting draft assembly (query) sequences into longer sequences. Gaps (stretches of "N" characters)
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
488 are placed between adjacent query sequences to indicate the presence of unknown sequence. RagTag uses whole-genome alignments to a reference
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
489 assembly to scaffold query sequences. RagTag does not alter input query sequence in any way and only orders and orients sequences, joining them with gaps.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
490
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
491 ----
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
492
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
493 .. class:: infomark
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
494
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
495 **Patch mode**
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
496
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
497 This mode uses one genome assembly to *patch* another genome assembly. We define two types of patches:
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
498
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
499 - Fills are patches that fill assembly gaps. This process is like traditional gap-filling, though it uses an assembly instead of WGS sequencing reads.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
500 - Joins are patches that join distinct contigs. This is essentially scaffolding and gap-filling in a single step.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
501
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
502 ----
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
503
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
504 .. class:: infomark
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
505
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
506 **Merge mode**
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
507
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
508 Draft genome assemblies are often scaffolded multiple times using different approaches. For example, one might scaffold an assembly using different genome
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
iuc
parents:
diff changeset
509 maps (physical, linkage, Hi-C, etc.), different methods, or different method parameters. RagTag merge is a tool to merge and reconcile different scaffoldings
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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510 of the same assembly. In this way, one can leverage the advantages of multiple techniques to synergistically improve scaffolding.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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511
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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512 Most tools write scaffolding results in the AGP file format, which encodes adjacency and gap information in a plain text file. To run RagTag merge,
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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513 one must supply the assembly in FASTA format and at least two AGP files that define a scaffolding of the assembly. Each AGP file can optionally be
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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514 assigned a weight, allowing users to assign the relative influence of each AGP on the final result.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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515
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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516 If available, users can supply Hi-C alignments to the draft assembly to resolve conflicts in the merging graph. In this scenario, the input AGP
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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517 files are used to build the initial graph, but then Hi-C alignments are used to re-weight the graph before computing the scaffolding solution.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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518
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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519
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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520 **List of accepted restriction enzymes**
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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521
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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522 List of all accepted restriction enzymes and their restriction sites:
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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523
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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524 - HindIII: AAGCTT
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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525 - Sau3AI: GATC
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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526 - MboI: GATC
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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527 - DpnII: GATC
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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528 - HinfI: GA[ATCG]TC
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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529 - DdeI: CT[ATCG]AG
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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530 - MseI: TTAA
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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531
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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532 For RagTag, use a comma separated list of enzymes or sites (or a mix). For example:
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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533
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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534 - Arima Hi-C v1.0: *Sau3AI,HinfI* or *GATC,GA[ATCG]TC*
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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535 - Arima Hi-C v2.0: *Sau3AI,HinfI,DdeI,MseI* or *GATC,GA[ATCG]TC,CT[ATCG]AG,TTAA*
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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536
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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537 Note that for restriction sites, wildcards are represented with python regex syntax, not IUPAC ambiguity codes. e.g. '[ATCG]' instead of 'N'.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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538
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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539 Restriction enzymes are not necessarily the enzyme used for sample prep. Each is only a enzyme that cuts at the corresponding restriction site.
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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540
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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541 ]]> </help>
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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542 <expand macro="citations" />
a04e64efa43a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"
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543 </tool>